MD13G1202300.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009607
Physical Location & Seq
Reverse (-)
18137686 .. 18141697
4012 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1202300.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGGGGCATTCATTTCCAGGTTTTGGTTCATGTTGTTTCCTGCAAAAGAGTATAAGATAGTGGTGGTTGGGTTGGATAATGCTGGGAAAACCACTACCCTTTACAAATTACACTTGGGAGAGGTTGTCACCACAAACCCTACTGTAGGCAGCAACGTTGAAGAGCTTGTCTACAAGAACATTCGATTCGAGGTATGGGATCTTGGGGGACAAGAAAGACTGAGGACTTCATGGGCAACATATTACCGTGGAACTCACGCTATCATTGCGGTTATAGACAGTACAGATAGAGCCAGAATTTCAATAATGAAAGATGAACTGTTCAGGTTGCTGGCACATGAGGATCTTCAACATTCTGTTGTGCTGGTTTTTGCAAATAAGCAAGACCTCAAGGATGCAATGACCCCAGCAGAGATCACCGACACGCTCTCTCTGCACAGCATCAAGAATCATGACTGGCACATCCAAGCCTGTTGTGCCCTCACAGGAGACGGGCTGTACGATGGTCTAGGTTGGATTGCCCAGCACGTTACAGGGAAAGCGCCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

184

Amino Acids

20.59

Weight (kDa)

6.22

Isoelectric Point (pI)

26.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 176 2.2e-66 ADP-ribosylation factor family
SRPRB PF09439 18 - 143 5.3e-11 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 2.4e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 136 7.8e-12 Ras family
MMR_HSR1 PF01926 19 - 123 5.5e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 24
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 269
AclI AACGTT 1 cut(s) 156
AclWI GGATC 2 cut(s) 207, 351
AcsI RAATTY 1 cut(s) 297
AfaI GTAC 2 cut(s) 283, 500
AfiI CCNNNNNNNGG 2 cut(s) 24, 146
AgsI TTSAA 3 cut(s) 161, 303, 350
AjnI CCWGG 1 cut(s) 17
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
Alw26I GTCTC 1 cut(s) 483
AlwI GGATC 2 cut(s) 207, 351
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 297
AspLEI GCGC 1 cut(s) 544
AsuHPI GGTGA 2 cut(s) 121, 409
BaeGI GKGCMC 1 cut(s) 481
BbvI GCAGC 1 cut(s) 162
BccI CCATC 1 cut(s) 497
BciT130I CCWGG 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 483
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 144
BfoI RGCGCY 1 cut(s) 545
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 19
BmsI GCATC 2 cut(s) 385, 450
BpuEI CTTGAG 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 146
Bse1I ACTGG 1 cut(s) 461
Bse3DI GCAATG 2 cut(s) 264, 405
BseBI CCWGG 1 cut(s) 19
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 2 cut(s) 400, 462
BseLI CCNNNNNNNGG 2 cut(s) 24, 146
BseMI GCAATG 2 cut(s) 264, 405
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 461
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 1 cut(s) 162
BseYI CCCAGC 3 cut(s) 83, 406, 522
BsgI GTGCAG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 24, 146
BsmAI GTCTC 1 cut(s) 483
BsmBI CGTCTC 1 cut(s) 483
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 3 cut(s) 199, 343, 414
BspACI CCGC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 213
BspHI TCATGA 1 cut(s) 451
BspPI GGATC 2 cut(s) 207, 351
BspQI GCTCTTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 264, 405
BsrI ACTGG 1 cut(s) 461
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 3 cut(s) 199, 343, 414
Bst2UI CCWGG 1 cut(s) 19
Bst4CI ACNGT 4 cut(s) 145, 248, 281, 321
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 221
BstDSI CCRYGG 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 400, 462
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 3 cut(s) 202, 346, 417
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 3 cut(s) 199, 343, 414
BstMWI GCNNNNNNNGC 3 cut(s) 266, 433, 476
BstNI CCWGG 1 cut(s) 19
BstSCI CCNGG 1 cut(s) 17
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 1 cut(s) 162
BstX2I RGATCY 2 cut(s) 199, 343
BstYI RGATCY 2 cut(s) 199, 343
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 400, 462
Cac8I GCNNGC 1 cut(s) 333
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 544
Csp6I GTAC 2 cut(s) 282, 499
CviAII CATG 4 cut(s) 31, 231, 338, 452
CviJI RGCY 4 cut(s) 166, 293, 470, 496
CviKI_1 RGCY 4 cut(s) 166, 293, 470, 496
CviQI GTAC 2 cut(s) 282, 499
DdeI CTNAG 1 cut(s) 221
DpnI GATC 3 cut(s) 201, 345, 416
DpnII GATC 3 cut(s) 199, 343, 414
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
EcoNI CCTNNNNNAGG 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 17
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 4 cut(s) 34, 234, 341, 455
FaiI YATR 8 cut(s) 32, 54, 196, 232, 241, 275, 339, 453
FaqI GGGAC 1 cut(s) 222
FatI CATG 4 cut(s) 30, 230, 337, 451
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 2 cut(s) 407, 449
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 1 cut(s) 509
GlaI GCGC 1 cut(s) 543
GluI GCNGC 1 cut(s) 151
GsaI CCCAGC 3 cut(s) 87, 410, 526
HaeII RGCGCY 1 cut(s) 545
HhaI GCGC 1 cut(s) 544
Hin1II CATG 4 cut(s) 34, 234, 341, 455
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HinfI GANTC 2 cut(s) 186, 448
HphI GGTGA 2 cut(s) 121, 409
Hpy166II GTNNAC 1 cut(s) 172
Hpy188III TCNNGA 2 cut(s) 445, 452
Hpy8I GTNNAC 1 cut(s) 172
HpyCH4III ACNGT 4 cut(s) 145, 248, 281, 321
HpyCH4IV ACGT 2 cut(s) 156, 528
HpyCH4V TGCA 4 cut(s) 44, 374, 398, 436
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 433, 476
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 2 cut(s) 156, 528
Hsp92II CATG 4 cut(s) 34, 234, 341, 455
HspAI GCGC 1 cut(s) 542
Kzo9I GATC 3 cut(s) 199, 343, 414
LguI GCTCTTC 1 cut(s) 156
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 2 cut(s) 385, 450
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 156, 528
MaeIII GTNAC 2 cut(s) 127, 529
MalI GATC 3 cut(s) 201, 345, 416
MboI GATC 3 cut(s) 199, 343, 414
MboII GAAGA 2 cut(s) 173, 338
MflI RGATCY 2 cut(s) 199, 343
MhlI GDGCHC 1 cut(s) 481
MluCI AATT 2 cut(s) 107, 297
MmeI TCCRAC 2 cut(s) 54, 494
MnlI CCTC 6 cut(s) 115, 184, 216, 334, 398, 491
MspR9I CCNGG 1 cut(s) 19
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 1 cut(s) 19
MwoI GCNNNNNNNGC 3 cut(s) 266, 433, 476
NdeII GATC 3 cut(s) 199, 343, 414
NlaIII CATG 4 cut(s) 34, 234, 341, 455
NmuCI GTSAC 1 cut(s) 127
PagI TCATGA 1 cut(s) 451
PciSI GCTCTTC 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 186, 448
PflMI CCANNNNNTGG 1 cut(s) 24
PkrI GCNGC 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 17
PspFI CCCAGC 3 cut(s) 83, 406, 522
PspGI CCWGG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 199, 343
RsaI GTAC 2 cut(s) 283, 500
RsaNI GTAC 2 cut(s) 282, 499
SapI GCTCTTC 1 cut(s) 156
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 3 cut(s) 199, 343, 414
ScrFI CCNGG 1 cut(s) 19
SduI GDGCHC 1 cut(s) 481
SetI ASST 9 cut(s) 23, 126, 159, 168, 195, 329, 390, 514, 531
SfaNI GCATC 2 cut(s) 385, 450
SfcI CTRYAG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 2 cut(s) 107, 297
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 17
TaaI ACNGT 4 cut(s) 145, 248, 281, 321
TaiI ACGT 2 cut(s) 159, 531
TaqI TCGA 2 cut(s) 184, 189
TasI AATT 2 cut(s) 107, 297
TatI WGTACW 1 cut(s) 281
TfiI GAWTC 2 cut(s) 186, 448
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 4 cut(s) 19, 219, 323, 330
Van91I CCANNNNNTGG 1 cut(s) 24
XagI CCTNNNNNAGG 1 cut(s) 144
XapI RAATTY 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.