RLG00000010020
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009607
Physical Location & Seq
Forward (+)
61819178 .. 61820895
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010020

Sequence Viewer

Length: 552 bp
ATGGGGGCATTCATATCGAGGTTTTGGTTCATGTTGTTTCCTGCAAAAGAGTATAAGATTGTGGTTGTTGGGTTGGACAATGCGGGGAAGACAACTACCCTTTACAAATTACACTTGGGAGAGGTTGTCACTACGAACCCAACTGTAGGCAGCAATGTCGAAGAGCTTGTCTACAAGAACATTCGCTTTGAGGTGTGGGATCTGGGTGGACAAGATAGACTGAGGACTTCATGGGCAACATACTACCGTGGAACTCATGCTATCATTGTGGTAATAGACAGCACAGACAGGGCCAGAATTTCCATTATGAAGGACGAACTCTTCAGGTTGCTTGGACATGAGGATCTTCAACATTCAGTTGTACTGGTTTTTGCTAATAAACAAGATCTCAAGGATGCCATGACCCCAGCTGAGATCACCGACACTCTATCTCTTCACAGCATTAAGAATCACGACTGGCACATCCAAGCCTGCTGTGCCCTCAACGGGGATGGATTGTATGATGGCCTAGGTTGGATCGCCTCACGGGTTACCGGGAAAGCACCTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

184

Amino Acids

20.57

Weight (kDa)

6.37

Isoelectric Point (pI)

28.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 175 5.4e-66 ADP-ribosylation factor family
SRPRB PF09439 18 - 143 5.9e-11 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 6.2e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 133 1e-11 Ras family
MMR_HSR1 PF01926 19 - 123 7.4e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 83
AclWI GGATC 3 cut(s) 207, 351, 524
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 307
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 3 cut(s) 146, 486, 487
AgsI TTSAA 1 cut(s) 350
AluBI AGCT 2 cut(s) 166, 410
AluI AGCT 2 cut(s) 166, 410
AlwI GGATC 3 cut(s) 207, 351, 524
AoxI GGCC 2 cut(s) 291, 505
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 297
ArsI GACNNNNNNTTYG 2 cut(s) 153, 185
AspA2I CCTAGG 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 1 cut(s) 409
AvrII CCTAGG 1 cut(s) 508
BaeGI GKGCMC 1 cut(s) 481
BbsI GAAGAC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 162
BccI CCATC 2 cut(s) 485, 497
BcgI CGANNNNNNTGC 3 cut(s) 31, 139, 173
BcnI CCSGG 1 cut(s) 535
BfaI CTAG 2 cut(s) 509, 546
BfmI CTRYAG 1 cut(s) 144
BglII AGATCT 1 cut(s) 385
BisI GCNGC 1 cut(s) 151
BlnI CCTAGG 1 cut(s) 508
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 1 cut(s) 385
BpiI GAAGAC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 247, 508
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 486, 487
Bse1I ACTGG 2 cut(s) 369, 461
Bse3DI GCAATG 1 cut(s) 160
BseDI CCNNGG 2 cut(s) 247, 508
BseGI GGATG 3 cut(s) 400, 462, 496
BseLI CCNNNNNNNGG 3 cut(s) 146, 486, 487
BseMI GCAATG 1 cut(s) 160
BseMII CTCAG 2 cut(s) 212, 402
BseNI ACTGG 2 cut(s) 369, 461
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 1 cut(s) 162
BseYI CCCAGC 1 cut(s) 406
BshFI GGCC 2 cut(s) 293, 507
BsiSI CCGG 1 cut(s) 534
BslI CCNNNNNNNGG 3 cut(s) 146, 486, 487
BsmI GAATGC 1 cut(s) 8
BsnI GGCC 2 cut(s) 293, 507
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 5 cut(s) 199, 343, 385, 414, 516
BspACI CCGC 1 cut(s) 83
BspANI GGCC 2 cut(s) 293, 507
BspCNI CTCAG 2 cut(s) 213, 403
BspPI GGATC 3 cut(s) 207, 351, 524
BspQI GCTCTTC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 160
BsrI ACTGG 2 cut(s) 369, 461
BssECI CCNNGG 2 cut(s) 247, 508
BssMI GATC 5 cut(s) 199, 343, 385, 414, 516
BssT1I CCWWGG 1 cut(s) 508
Bst4CI ACNGT 2 cut(s) 145, 248
Bst6I CTCTTC 3 cut(s) 156, 326, 438
BstC8I GCNNGC 1 cut(s) 472
BstDEI CTNAG 2 cut(s) 221, 411
BstDSI CCRYGG 1 cut(s) 247
BstEII GGTNACC 1 cut(s) 529
BstF5I GGATG 3 cut(s) 400, 462, 496
BstKTI GATC 5 cut(s) 202, 346, 388, 417, 519
BstMBI GATC 5 cut(s) 199, 343, 385, 414, 516
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstPI GGTNACC 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 533
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 3 cut(s) 199, 343, 385
BstYI RGATCY 3 cut(s) 199, 343, 385
BsuRI GGCC 2 cut(s) 293, 507
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 3 cut(s) 400, 462, 496
Cac8I GCNNGC 1 cut(s) 472
Cfr13I GGNCC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 362
CviAII CATG 5 cut(s) 31, 231, 257, 338, 400
CviJI RGCY 5 cut(s) 166, 293, 410, 470, 507
CviKI_1 RGCY 5 cut(s) 166, 293, 410, 470, 507
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 2 cut(s) 221, 411
DpnI GATC 5 cut(s) 201, 345, 387, 416, 518
DpnII GATC 5 cut(s) 199, 343, 385, 414, 516
Eam1104I CTCTTC 3 cut(s) 156, 326, 438
EarI CTCTTC 3 cut(s) 156, 326, 438
Eco130I CCWWGG 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 307
Eco91I GGTNACC 1 cut(s) 529
EcoO65I GGTNACC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 5 cut(s) 34, 234, 260, 341, 403
FatI CATG 5 cut(s) 30, 230, 256, 337, 399
FauI CCCGC 1 cut(s) 76
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 3 cut(s) 407, 449, 503
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 2 cut(s) 509, 546
GluI GCNGC 1 cut(s) 151
GsaI CCCAGC 1 cut(s) 410
HaeIII GGCC 2 cut(s) 293, 507
HapII CCGG 1 cut(s) 534
Hin1II CATG 5 cut(s) 34, 234, 260, 341, 403
HinfI GANTC 1 cut(s) 448
HpaII CCGG 1 cut(s) 534
HphI GGTGA 1 cut(s) 409
Hpy166II GTNNAC 2 cut(s) 172, 209
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 2 cut(s) 172, 209
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 2 cut(s) 145, 248
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 2 cut(s) 221, 411
Hsp92II CATG 5 cut(s) 34, 234, 260, 341, 403
Kzo9I GATC 5 cut(s) 199, 343, 385, 414, 516
LguI GCTCTTC 1 cut(s) 156
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 1 cut(s) 385
MaeI CTAG 2 cut(s) 509, 546
MaeIII GTNAC 2 cut(s) 127, 529
MalI GATC 5 cut(s) 201, 345, 387, 416, 518
MboI GATC 5 cut(s) 199, 343, 385, 414, 516
MboII GAAGA 5 cut(s) 100, 173, 313, 338, 425
MflI RGATCY 3 cut(s) 199, 343, 385
MhlI GDGCHC 1 cut(s) 481
MluCI AATT 2 cut(s) 107, 297
MmeI TCCRAC 2 cut(s) 54, 494
MnlI CCTC 7 cut(s) 12, 115, 184, 216, 334, 491, 532
MseI TTAA 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 1 cut(s) 535
Mva1269I GAATGC 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 476
NciI CCSGG 1 cut(s) 535
NdeII GATC 5 cut(s) 199, 343, 385, 414, 516
NlaIII CATG 5 cut(s) 34, 234, 260, 341, 403
NmuCI GTSAC 1 cut(s) 127
PciSI GCTCTTC 1 cut(s) 156
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 448
PkrI GCNGC 1 cut(s) 152
PspEI GGTNACC 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 406
PspPI GGNCC 1 cut(s) 291
PsuI RGATCY 3 cut(s) 199, 343, 385
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 5 cut(s) 199, 343, 385, 414, 516
Sau96I GGNCC 1 cut(s) 291
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 1 cut(s) 481
SetI ASST 8 cut(s) 23, 126, 168, 195, 329, 412, 514, 547
SfaNI GCATC 1 cut(s) 385
SfcI CTRYAG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 2 cut(s) 107, 297
SsiI CCGC 1 cut(s) 83
SspMI CTAG 2 cut(s) 509, 546
StyD4I CCNGG 1 cut(s) 533
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 2 cut(s) 145, 248
TaqI TCGA 2 cut(s) 17, 159
TasI AATT 2 cut(s) 107, 297
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 19, 219, 323
XapI RAATTY 1 cut(s) 297
XmaJI CCTAGG 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 2 cut(s) 509, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.