MD16G1202500.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009607
Physical Location & Seq
Reverse (-)
18420952 .. 18423773
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1202500.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGGGGCATTCATTTCCAAGTTTTGGTTCATGTTGTTTCCTGCAAAAGAGTATAAGATAGTGGTAGTTGGGTTGGACAATGCTGGGAAGACCACTACTCTTTACAAATTACACTTGGGAGAGGTTGTCACTACGAACCCAACTGTAGGCAGCAACGTCGAAGAGCTTGTCTACAAGAACATTCGATTCGAGGTATGGGATCTTGGTGGACAAGAAAGACTGAGGACTTCATGGGCAACATATTACCGTGGAACACATGCTATCATTGCGGTTATAGACAGTACGGATAGGGCAAGAATTTCAGTTATGAAAGATGAACTGTTGAGGTTGCTGGCACATGAGGATCTTCAACATTCTGTTGTACTGGTTTTTGCCAATAAACAAGACCTCAAGGATGCAATGACCCCAGCAGAGATCACCGACACACTCTCTCTTCACAGCATCAAGAATCATGACTGGCACATCCAAGCCTGTTGTGCCCTCACAGGAGAAGGGCTGTATGATGGTCTAGGTTGGATTGCCCAGCACGTTACAGGGAAAGCGCCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

184

Amino Acids

20.53

Weight (kDa)

6.22

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 176 1.3e-66 ADP-ribosylation factor family
SRPRB PF09439 18 - 143 2.2e-11 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 1.8e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 141 4.4e-12 Ras family
MMR_HSR1 PF01926 19 - 123 3.8e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 24
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 269
AclWI GGATC 2 cut(s) 207, 351
AcsI RAATTY 1 cut(s) 297
AfaI GTAC 2 cut(s) 283, 363
AfiI CCNNNNNNNGG 2 cut(s) 24, 146
AgsI TTSAA 1 cut(s) 350
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
AlwI GGATC 2 cut(s) 207, 351
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 297
ArsI GACNNNNNNTTYG 2 cut(s) 153, 185
AspLEI GCGC 1 cut(s) 544
AsuHPI GGTGA 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 481
BbsI GAAGAC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 162
BccI CCATC 1 cut(s) 497
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 144
BfoI RGCGCY 1 cut(s) 545
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
BmsI GCATC 2 cut(s) 385, 450
BpiI GAAGAC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 146
Bse1I ACTGG 2 cut(s) 369, 461
Bse3DI GCAATG 2 cut(s) 264, 405
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 2 cut(s) 400, 462
BseLI CCNNNNNNNGG 2 cut(s) 24, 146
BseMI GCAATG 2 cut(s) 264, 405
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 2 cut(s) 369, 461
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 1 cut(s) 162
BseYI CCCAGC 3 cut(s) 83, 406, 522
BslI CCNNNNNNNGG 2 cut(s) 24, 146
BsmI GAATGC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 3 cut(s) 199, 343, 414
BspACI CCGC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 213
BspHI TCATGA 1 cut(s) 451
BspPI GGATC 2 cut(s) 207, 351
BspQI GCTCTTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 264, 405
BsrI ACTGG 2 cut(s) 369, 461
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 3 cut(s) 199, 343, 414
Bst4CI ACNGT 4 cut(s) 145, 248, 281, 321
Bst6I CTCTTC 2 cut(s) 156, 438
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 221
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 2 cut(s) 400, 462
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 3 cut(s) 202, 346, 417
BstMBI GATC 3 cut(s) 199, 343, 414
BstMWI GCNNNNNNNGC 2 cut(s) 266, 476
BstNSI RCATGY 1 cut(s) 260
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 1 cut(s) 162
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 2 cut(s) 199, 343
BstYI RGATCY 2 cut(s) 199, 343
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 400, 462
Cac8I GCNNGC 1 cut(s) 333
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 544
Csp6I GTAC 2 cut(s) 282, 362
CviAII CATG 5 cut(s) 31, 231, 257, 338, 452
CviJI RGCY 3 cut(s) 166, 470, 496
CviKI_1 RGCY 3 cut(s) 166, 470, 496
CviQI GTAC 2 cut(s) 282, 362
DdeI CTNAG 1 cut(s) 221
DpnI GATC 3 cut(s) 201, 345, 416
DpnII GATC 3 cut(s) 199, 343, 414
Eam1104I CTCTTC 2 cut(s) 156, 438
EarI CTCTTC 2 cut(s) 156, 438
FaeI CATG 5 cut(s) 34, 234, 260, 341, 455
FatI CATG 5 cut(s) 30, 230, 256, 337, 451
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 2 cut(s) 407, 449
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 1 cut(s) 509
GlaI GCGC 1 cut(s) 543
GluI GCNGC 1 cut(s) 151
GsaI CCCAGC 3 cut(s) 87, 410, 526
HaeII RGCGCY 1 cut(s) 545
HhaI GCGC 1 cut(s) 544
Hin1II CATG 5 cut(s) 34, 234, 260, 341, 455
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HinfI GANTC 2 cut(s) 186, 448
HphI GGTGA 1 cut(s) 409
Hpy166II GTNNAC 2 cut(s) 172, 209
Hpy188III TCNNGA 2 cut(s) 445, 452
Hpy8I GTNNAC 2 cut(s) 172, 209
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 4 cut(s) 145, 248, 281, 321
HpyCH4IV ACGT 2 cut(s) 156, 528
HpyCH4V TGCA 2 cut(s) 44, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 476
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 2 cut(s) 156, 528
Hsp92II CATG 5 cut(s) 34, 234, 260, 341, 455
HspAI GCGC 1 cut(s) 542
Kzo9I GATC 3 cut(s) 199, 343, 414
LguI GCTCTTC 1 cut(s) 156
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 2 cut(s) 385, 450
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 156, 528
MaeIII GTNAC 2 cut(s) 127, 529
MalI GATC 3 cut(s) 201, 345, 416
MboI GATC 3 cut(s) 199, 343, 414
MboII GAAGA 4 cut(s) 100, 173, 338, 425
MflI RGATCY 2 cut(s) 199, 343
MhlI GDGCHC 1 cut(s) 481
MluCI AATT 2 cut(s) 107, 297
MmeI TCCRAC 2 cut(s) 54, 494
MnlI CCTC 7 cut(s) 115, 184, 216, 318, 334, 398, 491
Mva1269I GAATGC 1 cut(s) 8
MwoI GCNNNNNNNGC 2 cut(s) 266, 476
NdeII GATC 3 cut(s) 199, 343, 414
NlaIII CATG 5 cut(s) 34, 234, 260, 341, 455
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 260
PagI TCATGA 1 cut(s) 451
PciSI GCTCTTC 1 cut(s) 156
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 186, 448
PflMI CCANNNNNTGG 1 cut(s) 24
PkrI GCNGC 1 cut(s) 152
PspFI CCCAGC 3 cut(s) 83, 406, 522
PsuI RGATCY 2 cut(s) 199, 343
RsaI GTAC 2 cut(s) 283, 363
RsaNI GTAC 2 cut(s) 282, 362
SapI GCTCTTC 1 cut(s) 156
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 3 cut(s) 199, 343, 414
SduI GDGCHC 1 cut(s) 481
SetI ASST 8 cut(s) 126, 159, 168, 195, 329, 390, 514, 531
SfaNI GCATC 2 cut(s) 385, 450
SfcI CTRYAG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 2 cut(s) 107, 297
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 509
TaaI ACNGT 4 cut(s) 145, 248, 281, 321
TaiI ACGT 2 cut(s) 159, 531
TaqI TCGA 3 cut(s) 159, 184, 189
TasI AATT 2 cut(s) 107, 297
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 2 cut(s) 186, 448
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 4 cut(s) 19, 219, 323, 330
TspGWI ACGGA 1 cut(s) 299
Van91I CCANNNNNTGG 1 cut(s) 24
XapI RAATTY 1 cut(s) 297
XceI RCATGY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.