AT3G24420

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014310
Physical Location & Seq
Reverse (-)
8862813 .. 8865239
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24420.1

Sequence Viewer

Length: 822 bp
ATGGTGGTTAATCAGAAGATATCCGGCCTTGCATCCGCTATGAACGCCAAAATCATCGGTTCAGGAGAGAGATCGATGGTCTTGGCACACGGTTTCGGAGGCGACCAGTCGGTTTGGGATAAAATAATACCGGTCTTGTCCCAATCCTTCAAAGTTTTGGTCTTTGACTGGCTTTTCTCTGGAGCCATTAAAGATCAAACTCTCTATGACCCTTCAAAGTATAACTCCTTAGACGTCTTCTCTGACGATCTCATTGCTCTTATGGAGGAGTTGAAGTTTGGCCCTGTCGTGTTCGTAGGCCATTCCATGTCGGGGGTGATCGGTTGTGCTGCTTCTATTAAAAGGCCCGACTTGTTTACAAATCTTCTCCTTATTGCTGCTTCTCCAAGGTATATAAACAGTGAGGATTACAAAGGAGGGTTTGAGTCAAAAGACATTGACACGATCATTACCAGCATTGGCTCCAACTACGAAGCTTGGGCGGTTGATTTCTCCTCATTCGTGGTCGACTCGAGAGACTCTCTCTCGGTCCAAAGGTTCGAGAAGTCCCTTAAGAAGATGAAGCCCGAGACAGCTCTTGCCTTAGCTAAGATAGTGTTTGGTAGCGACGAGAGAGAGATTTTGGGACAAGTGTCAGTGCCTTGCCATGTCATACAACCAGGAAACGACGTCGTAGTGCCTGTCTCCGTCGCATACTTCATGCAGGAGAAGATTAAGGGGAAATCGACAGTGGAGATCATCGAGGACGCGATCGGGCATTTTCCACAGATGACATCACATTTGGAGCTGCTTGGCGTCATGAGGCGCCTCCTTGAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

273

Amino Acids

30.02

Weight (kDa)

5.29

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 25 - 246 5e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 26 - 258 8.3e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 26 - 258 4.3e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24420
fragaria_vesca FvH4_1g05320
malus_domestica MD02G1056400.v1.1 MD15G1189400.v1.1
prunus_persica Prupe.7G227500_v2.0.a1
pyrus_communis pycom02g04550 pycom15g16980
rosa_chinensis RchiOBHm_Chr2g0091021
rosa_laevigata RLG00000016167
rosa_multiflora Rmu_sc0003305.1_g000006
rosa_roxburghii Rroxscaffold_2G00150680
rosa_rugosa Rorug02G0011500
rosa_samantha Rh2AG057900 Rh2BG056200 Rh2CG058500 Rh2DG057600
rosa_wichuraiana Rw2G005140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 237, 672
AccB1I GGYRCC 1 cut(s) 804
AccI GTMKAC 1 cut(s) 507
AccII CGCG 1 cut(s) 749
AciI CCGC 2 cut(s) 36, 482
AcyI GRCGYC 4 cut(s) 234, 669, 795, 805
AfiI CCNNNNNNNGG 1 cut(s) 312
AflII CTTAAG 1 cut(s) 551
AgeI ACCGGT 1 cut(s) 130
AgsI TTSAA 3 cut(s) 151, 216, 274
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 4 cut(s) 476, 575, 587, 787
AluI AGCT 4 cut(s) 476, 575, 587, 787
Alw26I GTCTC 3 cut(s) 510, 563, 688
Ama87I CYCGRG 2 cut(s) 511, 566
AoxI GGCC 4 cut(s) 25, 280, 298, 344
ApeKI GCWGC 3 cut(s) 329, 377, 787
ArsI GACNNNNNNTTYG 4 cut(s) 144, 176, 763, 795
AsiGI ACCGGT 1 cut(s) 130
AspLEI GCGC 1 cut(s) 807
AspS9I GGNCC 3 cut(s) 281, 345, 529
AsuHPI GGTGA 1 cut(s) 328
AvaI CYCGRG 2 cut(s) 511, 566
AvaII GGWCC 1 cut(s) 529
BanI GGYRCC 1 cut(s) 804
BbsI GAAGAC 1 cut(s) 229
BbvI GCAGC 3 cut(s) 316, 364, 774
BccI CCATC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 1 cut(s) 660
BcoDI GTCTC 3 cut(s) 510, 563, 688
BfoI RGCGCY 1 cut(s) 808
BfrI CTTAAG 1 cut(s) 551
BisI GCNGC 3 cut(s) 330, 378, 788
BlsI GCNGC 3 cut(s) 331, 379, 789
Bme1390I CCNGG 1 cut(s) 660
Bme18I GGWCC 1 cut(s) 529
BmeT110I CYCGRG 2 cut(s) 511, 566
BmgT120I GGNCC 3 cut(s) 281, 345, 529
BmiI GGNNCC 3 cut(s) 184, 463, 806
BmrFI CCNGG 1 cut(s) 660
BmsI GCATC 1 cut(s) 41
BoxI GACNNNNGTC 1 cut(s) 631
BpiI GAAGAC 1 cut(s) 229
BplI GAGNNNNNCTC 4 cut(s) 505, 537, 507, 539
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 583
Bsa29I ATCGAT 1 cut(s) 74
BsaHI GRCGYC 4 cut(s) 234, 669, 795, 805
BsaJI CCNNGG 1 cut(s) 386
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 106, 173
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 660
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 1 cut(s) 32
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 252
BseNI ACTGG 2 cut(s) 106, 173
BseRI GAGGAG 2 cut(s) 281, 484
BseXI GCAGC 3 cut(s) 316, 364, 774
Bsh1236I CGCG 1 cut(s) 749
Bsh1285I CGRYCG 1 cut(s) 753
BshFI GGCC 4 cut(s) 27, 282, 300, 346
BshNI GGYRCC 1 cut(s) 804
BshTI ACCGGT 1 cut(s) 130
BshVI ATCGAT 1 cut(s) 74
BsiEI CGRYCG 1 cut(s) 753
BsiHKCI CYCGRG 2 cut(s) 511, 566
BsiSI CCGG 2 cut(s) 24, 131
BslFI GGGAC 3 cut(s) 124, 532, 639
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 3 cut(s) 510, 563, 688
BsmFI GGGAC 3 cut(s) 124, 532, 639
BsnI GGCC 4 cut(s) 27, 282, 300, 346
BsoBI CYCGRG 2 cut(s) 511, 566
Bsp143I GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
BspACI CCGC 2 cut(s) 36, 482
BspANI GGCC 4 cut(s) 27, 282, 300, 346
BspDI ATCGAT 1 cut(s) 74
BspFNI CGCG 1 cut(s) 749
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 3 cut(s) 184, 463, 806
BspT107I GGYRCC 1 cut(s) 804
BspTI CTTAAG 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 252
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 106, 173
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
BssNI GRCGYC 4 cut(s) 234, 669, 795, 805
BssT1I CCWWGG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 3 cut(s) 92, 401, 730
BstACI GRCGYC 4 cut(s) 234, 669, 795, 805
BstAFI CTTAAG 1 cut(s) 551
BstDEI CTNAG 3 cut(s) 229, 583, 588
BstF5I GGATG 1 cut(s) 32
BstFNI CGCG 1 cut(s) 749
BstH2I RGCGCY 1 cut(s) 808
BstHHI GCGC 1 cut(s) 807
BstKTI GATC 7 cut(s) 74, 196, 250, 321, 447, 738, 753
BstMAI GTCTC 3 cut(s) 510, 563, 688
BstMBI GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
BstMCI CGRYCG 1 cut(s) 753
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 1 cut(s) 660
BstPAI GACNNNNGTC 1 cut(s) 631
BstSCI CCNGG 1 cut(s) 658
BstUI CGCG 1 cut(s) 749
BstV1I GCAGC 3 cut(s) 316, 364, 774
BstV2I GAAGAC 1 cut(s) 229
Bsu15I ATCGAT 1 cut(s) 74
BsuRI GGCC 4 cut(s) 27, 282, 300, 346
BsuTUI ATCGAT 1 cut(s) 74
BtsCI GGATG 1 cut(s) 32
BtsIMutI CAGTG 3 cut(s) 406, 642, 735
CciI TCATGA 1 cut(s) 798
CfoI GCGC 1 cut(s) 807
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 3 cut(s) 281, 345, 529
ClaI ATCGAT 1 cut(s) 74
CseI GACGC 2 cut(s) 755, 784
CspAI ACCGGT 1 cut(s) 130
CviAII CATG 4 cut(s) 307, 647, 700, 799
DdeI CTNAG 3 cut(s) 229, 583, 588
DinI GGCGCC 1 cut(s) 806
DpnI GATC 7 cut(s) 73, 195, 249, 320, 446, 737, 752
DpnII GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
Eco130I CCWWGG 1 cut(s) 386
Eco32I GATATC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 529
Eco88I CYCGRG 2 cut(s) 511, 566
EcoRII CCWGG 1 cut(s) 658
EcoRV GATATC 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 386
EgeI GGCGCC 1 cut(s) 806
EheI GGCGCC 1 cut(s) 806
ErhI CCWWGG 1 cut(s) 386
FaeI CATG 4 cut(s) 310, 650, 703, 802
FaqI GGGAC 3 cut(s) 124, 532, 639
FatI CATG 4 cut(s) 306, 646, 699, 798
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 330, 378, 788
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 3 cut(s) 330, 378, 788
GlaI GCGC 1 cut(s) 806
GluI GCNGC 3 cut(s) 330, 378, 788
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 1 cut(s) 808
HaeIII GGCC 4 cut(s) 27, 282, 300, 346
HapII CCGG 2 cut(s) 24, 131
HgaI GACGC 2 cut(s) 755, 784
HhaI GCGC 1 cut(s) 807
Hin1I GRCGYC 4 cut(s) 234, 669, 795, 805
Hin1II CATG 4 cut(s) 310, 650, 703, 802
Hin6I GCGC 1 cut(s) 805
HinP1I GCGC 1 cut(s) 805
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 3 cut(s) 425, 509, 518
HpaII CCGG 2 cut(s) 24, 131
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 357, 508
Hpy188I TCNGA 3 cut(s) 15, 98, 244
Hpy188III TCNNGA 5 cut(s) 63, 180, 513, 541, 799
Hpy8I GTNNAC 2 cut(s) 357, 508
Hpy99I CGWCG 4 cut(s) 611, 671, 674, 692
HpyAV CCTTC 2 cut(s) 157, 222
HpyCH4III ACNGT 3 cut(s) 92, 401, 730
HpyCH4IV ACGT 2 cut(s) 234, 669
HpyCH4V TGCA 2 cut(s) 32, 703
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 3 cut(s) 229, 583, 588
HpySE526I ACGT 2 cut(s) 234, 669
Hsp92I GRCGYC 4 cut(s) 234, 669, 795, 805
Hsp92II CATG 4 cut(s) 310, 650, 703, 802
HspAI GCGC 1 cut(s) 805
KasI GGCGCC 1 cut(s) 804
Kzo9I GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
LmnI GCTCC 3 cut(s) 182, 467, 784
Lsp1109I GCAGC 3 cut(s) 316, 364, 774
LweI GCATC 1 cut(s) 41
MaeII ACGT 2 cut(s) 234, 669
MalI GATC 7 cut(s) 73, 195, 249, 320, 446, 737, 752
MboI GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
MboII GAAGA 5 cut(s) 28, 229, 356, 568, 721
Mly113I GGCGCC 1 cut(s) 805
MlyI GAGTC 3 cut(s) 434, 503, 512
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 8 cut(s) 92, 259, 397, 410, 505, 736, 795, 818
MseI TTAA 6 cut(s) 9, 189, 339, 552, 714, 820
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 2 cut(s) 24, 131
MspR9I CCNGG 1 cut(s) 660
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 1 cut(s) 749
MwoI GCNNNNNNNGC 1 cut(s) 44
NarI GGCGCC 1 cut(s) 805
NdeII GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
NlaIII CATG 4 cut(s) 310, 650, 703, 802
NlaIV GGNNCC 3 cut(s) 184, 463, 806
PaeR7I CTCGAG 1 cut(s) 511
PagI TCATGA 1 cut(s) 798
PinAI ACCGGT 1 cut(s) 130
PkrI GCNGC 3 cut(s) 331, 379, 789
Ple19I CGATCG 1 cut(s) 753
PleI GAGTC 3 cut(s) 433, 503, 512
PluTI GGCGCC 1 cut(s) 808
PpsI GAGTC 3 cut(s) 433, 503, 512
PshAI GACNNNNGTC 1 cut(s) 631
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 3 cut(s) 184, 463, 806
PspPI GGNCC 3 cut(s) 281, 345, 529
PvuI CGATCG 1 cut(s) 753
SalI GTCGAC 1 cut(s) 506
SaqAI TTAA 6 cut(s) 9, 189, 339, 552, 714, 820
SatI GCNGC 3 cut(s) 330, 378, 788
Sau3AI GATC 7 cut(s) 71, 193, 247, 318, 444, 735, 750
Sau96I GGNCC 3 cut(s) 281, 345, 529
SchI GAGTC 3 cut(s) 434, 503, 512
ScrFI CCNGG 1 cut(s) 660
SetI ASST 8 cut(s) 237, 392, 478, 539, 577, 589, 672, 789
SfaNI GCATC 1 cut(s) 41
SfoI GGCGCC 1 cut(s) 806
Sfr274I CTCGAG 1 cut(s) 511
SinI GGWCC 1 cut(s) 529
SlaI CTCGAG 1 cut(s) 511
SmlI CTYRAG 3 cut(s) 511, 551, 812
SmoI CTYRAG 3 cut(s) 511, 551, 812
SsiI CCGC 2 cut(s) 36, 482
SspDI GGCGCC 1 cut(s) 804
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 3 cut(s) 92, 401, 730
TaiI ACGT 2 cut(s) 237, 672
TaqI TCGA 6 cut(s) 74, 507, 512, 540, 725, 741
TaqII GACCGA 1 cut(s) 517
Tru1I TTAA 6 cut(s) 9, 189, 339, 552, 714, 820
Tru9I TTAA 6 cut(s) 9, 189, 339, 552, 714, 820
TscAI CASTG 3 cut(s) 406, 642, 735
TseI GCWGC 3 cut(s) 329, 377, 787
TspDTI ATGAA 3 cut(s) 56, 575, 688
TspGWI ACGGA 1 cut(s) 676
TspRI CASTG 3 cut(s) 406, 642, 735
Vha464I CTTAAG 1 cut(s) 551
VpaK11BI GGWCC 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 507
ZraI GACGTC 2 cut(s) 235, 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.