Rmu_sc0003305.1_g000006

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003305.1
OG0014310
Physical Location & Seq
Forward (+)
36111 .. 37591
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003305.1_g000006.1.cds

Sequence Viewer

Length: 831 bp
atgaacgctaaaatcgtgggcacggggagtgagtccatagtcttagcacatggctatgggggagaccagtctgtgtgggacaaaatccttccctacttggctcaacattacagtgttcttgttttcgactggaccttttcgggtgctgctaaggaccccaacctctttgatcctgccaaatattcgagctacgatgattttgctcatgacttgattgctctattggatgaacttaacttgaaatcctcagtctatgttggccactccatgtctggtatggttggatgcattgcttccatcaagagacctgacctctttagcagcctcatacttcttggagcttctcctaggtatatgaatacagatgactatgaaggtgggtttgggagttcagacattaaacaaatcctctcaagcatagaatccaactttcacaactgggctgctcactttgctccccttgttgtggactcaaatgaccctctctctgttgacaagtttaccaagtgcctgcaaaaaatgagacctgaatttgcacttgctgtaggaaaaatcgtcttttacagcgacgaaagagacactctcgacaaggtctcaactccatgcactatcatccacaccagcagtgacattgtctctccaaaatcggtcgcgttttacatgcaaaagaaaatcaaggaaaaccccaaagtggagattatgaatactaatggccatttcccacagttgactgcacacattcagcttcttgatgtgctaggactcatcttgggggttgaattggatcttgatattgatcttaaaccaagattgggcaaattatcatgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

276

Amino Acids

30.44

Weight (kDa)

5.41

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24420
fragaria_vesca FvH4_1g05320
malus_domestica MD02G1056400.v1.1 MD15G1189400.v1.1
prunus_persica Prupe.7G227500_v2.0.a1
pyrus_communis pycom02g04550 pycom15g16980
rosa_chinensis RchiOBHm_Chr2g0091021
rosa_laevigata RLG00000016167
rosa_multiflora Rmu_sc0003305.1_g000006
rosa_roxburghii Rroxscaffold_2G00150680
rosa_rugosa Rorug02G0011500
rosa_samantha Rh2AG057900 Rh2BG056200 Rh2CG058500 Rh2DG057600
rosa_wichuraiana Rw2G005140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 653
AclWI GGATC 2 cut(s) 164, 792
AcoI YGGCCR 2 cut(s) 259, 712
AcsI RAATTY 1 cut(s) 530
AfiI CCNNNNNNNGG 3 cut(s) 466, 691, 812
AgsI TTSAA 2 cut(s) 241, 779
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AluBI AGCT 3 cut(s) 189, 341, 745
AluI AGCT 3 cut(s) 189, 341, 745
Alw26I GTCTC 6 cut(s) 57, 298, 517, 570, 598, 640
AlwI GGATC 2 cut(s) 164, 792
AoxI GGCC 2 cut(s) 259, 712
ApeKI GCWGC 3 cut(s) 146, 321, 443
ApoI RAATTY 1 cut(s) 530
AspA2I CCTAGG 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 132, 154
AvaII GGWCC 2 cut(s) 132, 154
AvrII CCTAGG 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 23
BalI TGGCCA 2 cut(s) 261, 714
BbvI GCAGC 3 cut(s) 133, 333, 430
BccI CCATC 1 cut(s) 305
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BcoDI GTCTC 6 cut(s) 57, 298, 517, 570, 598, 640
BfaI CTAG 2 cut(s) 348, 758
BfmI CTRYAG 1 cut(s) 543
BisI GCNGC 3 cut(s) 147, 322, 444
BlnI CCTAGG 1 cut(s) 347
BlsI GCNGC 3 cut(s) 148, 323, 445
Bme18I GGWCC 2 cut(s) 132, 154
BmgT120I GGNCC 2 cut(s) 132, 154
BmiI GGNNCC 1 cut(s) 156
BmrI ACTGGG 1 cut(s) 448
BmsI GCATC 1 cut(s) 275
BmuI ACTGGG 1 cut(s) 448
BplI GAGNNNNNCTC 2 cut(s) 567, 599
Bpu10I CCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 397
BsaBI GATNNNNATC 1 cut(s) 795
BsaI GGTCTC 4 cut(s) 57, 298, 517, 598
BsaJI CCNNGG 1 cut(s) 347
Bsc4I CCNNNNNNNGG 3 cut(s) 466, 691, 812
Bse1I ACTGG 3 cut(s) 67, 134, 443
Bse3DI GCAATG 1 cut(s) 288
Bse8I GATNNNNATC 1 cut(s) 795
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 3 cut(s) 232, 290, 612
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 3 cut(s) 466, 691, 812
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 3 cut(s) 67, 134, 443
BseSI GKGCMC 1 cut(s) 23
BseXI GCAGC 3 cut(s) 133, 333, 430
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 653
Bsh1285I CGRYCG 1 cut(s) 651
BshFI GGCC 2 cut(s) 261, 714
BsiEI CGRYCG 1 cut(s) 651
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 3 cut(s) 466, 691, 812
BsmAI GTCTC 6 cut(s) 57, 298, 517, 570, 598, 640
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 2 cut(s) 261, 714
Bso31I GGTCTC 4 cut(s) 57, 298, 517, 598
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 3 cut(s) 169, 784, 796
BspANI GGCC 2 cut(s) 261, 714
BspCNI CTCAG 1 cut(s) 260
BspFNI CGCG 1 cut(s) 653
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 2 cut(s) 164, 792
BspTNI GGTCTC 4 cut(s) 57, 298, 517, 598
BsrDI GCAATG 1 cut(s) 288
BsrI ACTGG 3 cut(s) 67, 134, 443
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 3 cut(s) 169, 784, 796
BssT1I CCWWGG 1 cut(s) 347
Bst4CI ACNGT 2 cut(s) 113, 726
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 3 cut(s) 43, 150, 247
BstF5I GGATG 3 cut(s) 232, 290, 612
BstFNI CGCG 1 cut(s) 653
BstKTI GATC 3 cut(s) 172, 787, 799
BstMAI GTCTC 6 cut(s) 57, 298, 517, 570, 598, 640
BstMBI GATC 3 cut(s) 169, 784, 796
BstMCI CGRYCG 1 cut(s) 651
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstNSI RCATGY 1 cut(s) 664
BstSFI CTRYAG 1 cut(s) 543
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 1 cut(s) 653
BstV1I GCAGC 3 cut(s) 133, 333, 430
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BsuRI GGCC 2 cut(s) 261, 714
BtsCI GGATG 3 cut(s) 232, 290, 612
BtsI GCAGTG 1 cut(s) 631
BtsIMutI CAGTG 2 cut(s) 118, 631
Cac8I GCNNGC 1 cut(s) 512
CciI TCATGA 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 132, 154
CviAII CATG 6 cut(s) 50, 206, 268, 603, 661, 825
CviJI RGCY 9 cut(s) 54, 101, 189, 261, 324, 341, 443, 714, 745
CviKI_1 RGCY 9 cut(s) 54, 101, 189, 261, 324, 341, 443, 714, 745
DdeI CTNAG 3 cut(s) 43, 150, 247
DpnI GATC 3 cut(s) 171, 786, 798
DpnII GATC 3 cut(s) 169, 784, 796
EaeI YGGCCR 2 cut(s) 259, 712
Eco130I CCWWGG 1 cut(s) 347
Eco31I GGTCTC 4 cut(s) 57, 298, 517, 598
Eco47I GGWCC 2 cut(s) 132, 154
EcoO109I RGGNCCY 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 347
EcoT22I ATGCAT 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 6 cut(s) 53, 209, 271, 606, 664, 828
FaqI GGGAC 1 cut(s) 92
FatI CATG 6 cut(s) 49, 205, 267, 602, 660, 824
Fnu4HI GCNGC 3 cut(s) 147, 322, 444
FokI GGATG 3 cut(s) 239, 297, 599
Fsp4HI GCNGC 3 cut(s) 147, 322, 444
FspBI CTAG 2 cut(s) 348, 758
GluI GCNGC 3 cut(s) 147, 322, 444
HaeIII GGCC 2 cut(s) 261, 714
Hin1II CATG 6 cut(s) 53, 209, 271, 606, 664, 828
HincII GTYRAC 2 cut(s) 493, 729
HindII GTYRAC 2 cut(s) 493, 729
HinfI GANTC 4 cut(s) 32, 422, 470, 762
Hpy166II GTNNAC 4 cut(s) 469, 493, 501, 729
Hpy188I TCNGA 1 cut(s) 394
Hpy188III TCNNGA 5 cut(s) 206, 301, 584, 749, 788
Hpy8I GTNNAC 4 cut(s) 469, 493, 501, 729
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 2 cut(s) 98, 368
HpyCH4III ACNGT 2 cut(s) 113, 726
HpyCH4V TGCA 6 cut(s) 288, 514, 536, 606, 664, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 3 cut(s) 43, 150, 247
Hsp92II CATG 6 cut(s) 53, 209, 271, 606, 664, 828
Kzo9I GATC 3 cut(s) 169, 784, 796
LmnI GCTCC 2 cut(s) 338, 460
LpnPI CCDG 9 cut(s) 80, 115, 186, 258, 321, 424, 524, 540, 634
Lsp1109I GCAGC 3 cut(s) 133, 333, 430
LweI GCATC 1 cut(s) 275
MaeI CTAG 2 cut(s) 348, 758
MaeIII GTNAC 1 cut(s) 626
MalI GATC 3 cut(s) 171, 786, 798
MboI GATC 3 cut(s) 169, 784, 796
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 1 cut(s) 23
MlsI TGGCCA 2 cut(s) 261, 714
MluCI AATT 3 cut(s) 530, 779, 818
MluNI TGGCCA 2 cut(s) 261, 714
MlyI GAGTC 3 cut(s) 41, 464, 756
MmeI TCCRAC 2 cut(s) 262, 450
MnlI CCTC 6 cut(s) 173, 256, 323, 335, 419, 492
Mox20I TGGCCA 2 cut(s) 261, 714
Mph1103I ATGCAT 1 cut(s) 290
MscI TGGCCA 2 cut(s) 261, 714
MseI TTAA 3 cut(s) 234, 399, 801
MslI CAYNNNNRTG 2 cut(s) 54, 111
Msp20I TGGCCA 2 cut(s) 261, 714
MvnI CGCG 1 cut(s) 653
MwoI GCNNNNNNNGC 1 cut(s) 452
NdeII GATC 3 cut(s) 169, 784, 796
NlaIII CATG 6 cut(s) 53, 209, 271, 606, 664, 828
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 626
NsiI ATGCAT 1 cut(s) 290
NspI RCATGY 1 cut(s) 664
PagI TCATGA 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 1 cut(s) 422
PflFI GACNNNGTC 2 cut(s) 590, 632
PkrI GCNGC 3 cut(s) 148, 323, 445
PleI GAGTC 3 cut(s) 40, 464, 756
PpsI GAGTC 3 cut(s) 40, 464, 756
PpuMI RGGWCCY 1 cut(s) 154
Psp5II RGGWCCY 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 132, 154
PspPPI RGGWCCY 1 cut(s) 154
PsuI RGATCY 1 cut(s) 784
PsyI GACNNNGTC 2 cut(s) 590, 632
RseI CAYNNNNRTG 2 cut(s) 54, 111
SaqAI TTAA 3 cut(s) 234, 399, 801
SatI GCNGC 3 cut(s) 147, 322, 444
Sau3AI GATC 3 cut(s) 169, 784, 796
Sau96I GGNCC 2 cut(s) 132, 154
SchI GAGTC 3 cut(s) 41, 464, 756
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 1 cut(s) 275
SfcI CTRYAG 1 cut(s) 543
SinI GGWCC 2 cut(s) 132, 154
SmiMI CAYNNNNRTG 2 cut(s) 54, 111
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 3 cut(s) 530, 779, 818
SspI AATATT 1 cut(s) 182
SspMI CTAG 2 cut(s) 348, 758
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 2 cut(s) 113, 726
TaqI TCGA 3 cut(s) 126, 185, 585
TaqII GACCGA 1 cut(s) 637
TasI AATT 3 cut(s) 530, 779, 818
TfiI GAWTC 1 cut(s) 422
Tru1I TTAA 3 cut(s) 234, 399, 801
Tru9I TTAA 3 cut(s) 234, 399, 801
TscAI CASTG 2 cut(s) 118, 631
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 3 cut(s) 146, 321, 443
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 5 cut(s) 17, 243, 371, 387, 716
TspRI CASTG 2 cut(s) 118, 631
Tth111I GACNNNGTC 2 cut(s) 590, 632
VpaK11BI GGWCC 2 cut(s) 132, 154
XapI RAATTY 1 cut(s) 530
XceI RCATGY 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 2 cut(s) 269, 274
XmaJI CCTAGG 1 cut(s) 347
XspI CTAG 2 cut(s) 348, 758
Zsp2I ATGCAT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.