Rorug02G0011500

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003536
Physical Location & Seq
Forward (+)
1028744 .. 1031459
2716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0011500.1

Sequence Viewer

Length: 351 bp
ATGGCCAATGAAGCTTTAACAGCACGAAACCCAGATGGGGTTCACACCGATGTGCTCTCTAGTGCAAGACAAGCTTGCTATAAGGCACGAGACGCATTCTATGCTTGTTTGGAGAAAGAATCAGATAAGAAACAGACTGAAATTGCGTCCAAGGGTCTGTTATACCCAGCACAGTGCAAACCCGCCAGGGATCAGTTCGTCAATAACTGCCGTGCTTCTTGGGTGAAGCATTTTGATGCGCTGCACTGCAAGAACAGAAGTTTGAAGAGGCTTTTGGATGATAAAGAATCGAGGAGAGGTCCGTTGTCACTTCCACAACCTTACACTTTCAAGCCCCCCAGCACTAGTTAA

Protein Analysis

116

Amino Acids

13.1

Weight (kDa)

9.32

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX6B PF02297 20 - 86 4.7e-15 Cytochrome oxidase c subunit VIb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 183
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 3
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 2 cut(s) 265, 331
AhlI ACTAGT 1 cut(s) 344
AjnI CCWGG 1 cut(s) 185
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 2 cut(s) 14, 74
AluI AGCT 2 cut(s) 14, 74
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 1 cut(s) 198
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 241
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 299
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 228
BccI CCATC 1 cut(s) 29
BceAI ACGGC 1 cut(s) 195
BciT130I CCWGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 84
BcuI ACTAGT 1 cut(s) 344
BfaI CTAG 2 cut(s) 60, 345
BisI GCNGC 1 cut(s) 242
BlsI GCNGC 1 cut(s) 243
Bme1390I CCNGG 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 299
BmrFI CCNGG 1 cut(s) 187
BmsI GCATC 1 cut(s) 226
BsaJI CCNNGG 2 cut(s) 150, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 150, 186
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 1 cut(s) 37
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 228
BseYI CCCAGC 2 cut(s) 166, 338
BsgI GTGCAG 1 cut(s) 227
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 84
BsmBI CGTCTC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 183
BspANI GGCC 1 cut(s) 5
BspPI GGATC 1 cut(s) 198
BssECI CCNNGG 2 cut(s) 150, 186
BssMI GATC 1 cut(s) 190
BssSI CACGAG 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 260
BstAPI GCANNNNNTGC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 76
BstF5I GGATG 1 cut(s) 283
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 71, 92, 101
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 228
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 283
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 2 cut(s) 179, 244
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 299
CseI GACGC 2 cut(s) 101, 135
CviJI RGCY 5 cut(s) 5, 14, 74, 271, 334
CviKI_1 RGCY 5 cut(s) 5, 14, 74, 271, 334
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 84
FaiI YATR 3 cut(s) 81, 102, 163
FalI AAGNNNNNCTT 2 cut(s) 58, 90
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 242
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 1 cut(s) 242
FspBI CTAG 2 cut(s) 60, 345
GlaI GCGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 242
GsaI CCCAGC 2 cut(s) 170, 342
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 101, 135
HhaI GCGC 1 cut(s) 241
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HindIII AAGCTT 2 cut(s) 12, 72
HinfI GANTC 2 cut(s) 119, 287
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 1 cut(s) 124
Hpy8I GTNNAC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 65, 177, 244, 249
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 71, 92, 101
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 1 cut(s) 190
LpnPI CCDG 4 cut(s) 45, 172, 180, 199
Lsp1109I GCAGC 1 cut(s) 228
LweI GCATC 1 cut(s) 226
MaeI CTAG 2 cut(s) 60, 345
MaeIII GTNAC 1 cut(s) 306
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 57
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 141
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 3 cut(s) 261, 285, 290
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 17, 349
MslI CAYNNNNRTG 3 cut(s) 48, 50, 234
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 187
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 1 cut(s) 187
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 71, 92, 101
NdeII GATC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 306
OliI CACNNNNGTG 1 cut(s) 50
PctI GAATGC 1 cut(s) 95
PfeI GAWTC 2 cut(s) 119, 287
PkrI GCNGC 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 185
PspFI CCCAGC 2 cut(s) 166, 338
PspGI CCWGG 1 cut(s) 185
PspPI GGNCC 1 cut(s) 299
RseI CAYNNNNRTG 3 cut(s) 48, 50, 234
SaqAI TTAA 2 cut(s) 17, 349
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 187
SduI GDGCHC 1 cut(s) 57
SetI ASST 4 cut(s) 16, 76, 301, 322
SfaNI GCATC 1 cut(s) 226
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 3 cut(s) 48, 50, 234
SpeI ACTAGT 1 cut(s) 344
Sse9I AATT 1 cut(s) 141
SsiI CCGC 1 cut(s) 183
SspMI CTAG 2 cut(s) 60, 345
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 290
TasI AATT 1 cut(s) 141
TfiI GAWTC 2 cut(s) 119, 287
Tru1I TTAA 2 cut(s) 17, 349
Tru9I TTAA 2 cut(s) 17, 349
TscAI CASTG 2 cut(s) 179, 251
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 24
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 179, 251
VpaK11BI GGWCC 1 cut(s) 299
XspI CTAG 2 cut(s) 60, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.