Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003536

Physical Location & Seq

Genomic Coordinates Forward (+)

4571258 .. 4575178

Gene Structure 3921 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1056500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGCGAAGTTTGTTCGGCGGAGCGACCCAAATTCTGAGCAAATCGTCGTCCGCTTTCTTCTTCCTCAATCCAAATTGGAAGCTCTCTCTTTGCGAACCGGGGTCCAAATCCCCTCGCCTTCTCCGCACCTTCGCGATCATGGCCTCCGCGGACGAGTTCGTCAAGGGCAGCGTCCACCCAAATGGCGTCGCCGTCATCACCCTGGATCGCCCCCGAGCTCTCAATGCCATGAACCTAGATATGGATGTGATATACAAGAGCTATCTGGATGAATGGGAATCTGACCCAAATGTGAAGTGTGTTCTTGTTGATAGCAGCTCGGCTCGTGCCTTTTGTGCCGGCGGTGATGTGAAGCAGATAACGGCCAAAAACCAACTACCGGATATGATTGAGGTATTCACTGCTGAGTATTCTCTAATATGCAAAATCTCTGAGTACAAGAAGCCCTATATAAGCTTCATGGATGGCATAACAATGGGCTTTGGTATTGGCCTATCAGGTCACGGTCGCTACCGGATCATTACAGAGAGGACTGTTCTTGCTATGCCAGAGAATGGAATTGGTTTGTTCCCAGATGTTGGGTTTTCACATATAGCGGCAAAGAGTCCTGGAGGAGGATCTGTAGGTGCTTATCTTGGACTTACTGGGAAAAGGATTTCAACACCATCGGATGCACTATATGTGGGTCTTGGGACACATTTTGTCCCATCTCAAAACTTAGGTTCTTTAAAGGAGGCCCTTTTGTCTACCAAATTTTCCCAGGATCCCCATCAGGACGTTGAAACAGCTGTGGCAAAATTCAGCAGCAACCCAGATTCTGAAGCGCAACTCAAGAATCTTTTACCTCGAATAACTTCAGCATTTGGTGCAAATAAGTCGGTTCTTGAGACAATTGATGAACTTAAAAAGCAGCAGCAGAGTTCAGATGCTACTGTGGTGGAGTGGGCTAATGAAGCTCTTCAAGGTCTTGGAAAGGGTGCTCCTTTTTCACTTTATTTGACACAAAATTATTTCTCCAAAGTTGCATCTGCGCTTGGGAAAAATGACAATCAATTATCCACATTAACTGGTGTGATGAAAACTGAATATCGCGTTGCTGTAAGATCGTCTTTGAGGAATGATTTTGCTGAAGGTGTGCGGGCAGTTTTGGTGGACAAGGATCAGAATCCAAAGTGGAACCCGGCAAAGTTAGAGGAGGTGAATCGGAGTGAAGTGGAAGCTCTCTTCGAGCCATTGAGCCCGAACGTTGAGGAGTTGAGTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016020 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

422

Amino Acids

45.76

Weight (kDa)

6.05

Isoelectric Point (pI)

31.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	60 - 266	2.7e-24	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	64 - 409	1.9e-106	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003536)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24360 AT3G24360 AT3G24360 AT3G24360 AT3G24360 AT3G24360 AT4G13360
fragaria_vesca	FvH4_1g05350 FvH4_1g05350 FvH4_1g05350 FvH4_1g05350 FvH4_1g05350 FvH4_1g05350
malus_domestica	MD02G1052700.v1.1 MD02G1056500.v1.1 MD15G1189500.v1.1
prunus_persica	Prupe.7G227300_v2.0.a1
pyrus_communis	pycom02g04570 pycom15g16990
rosa_chinensis	RchiOBHm_Chr2g0091031 RchiOBHm_Chr2g0092761 RchiOBHm_Chr2g0092831
rosa_laevigata	RLG00000016169
rosa_multiflora	Rmu_sc0003305.1_g000013
rosa_roxburghii	Rroxscaffold_2G00148870 Rroxscaffold_2G00150670
rosa_rugosa	Rorug02G0011500 Rorug02G0011500 Rorug02G0011600 Rorug02G0029300
rosa_samantha	Rh2AG058000 Rh2BG056300 Rh2CG058600 Rh2DG057800
rosa_wichuraiana	Rw2G005120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	158
AccB7I	CCANNNNNTGG	2 cut(s)	554, 578
AccI	GTMKAC	1 cut(s)	746
AccII	CGCG	3 cut(s)	134, 149, 1092
AciI	CCGC	8 cut(s)	18, 51, 124, 147, 149, 342, 596, 1138
AclI	AACGTT	1 cut(s)	1245
AclWI	GGATC	6 cut(s)	213, 524, 625, 758, 771, 1167
AcoI	YGGCCR	1 cut(s)	363
AcsI	RAATTY	3 cut(s)	30, 752, 797
AcuI	CTGAAG	3 cut(s)	840, 840, 1149
AcyI	GRCGYC	1 cut(s)	186
AfaI	GTAC	1 cut(s)	437
AfiI	CCNNNNNNNGG	5 cut(s)	241, 379, 554, 578, 614
AgsI	TTSAA	3 cut(s)	660, 782, 962
AjnI	CCWGG	3 cut(s)	201, 607, 759
AluBI	AGCT	8 cut(s)	82, 218, 261, 318, 456, 788, 956, 1220
AluI	AGCT	8 cut(s)	82, 218, 261, 318, 456, 788, 956, 1220
Alw21I	GWGCWC	2 cut(s)	220, 982
Alw26I	GTCTC	1 cut(s)	881
AlwI	GGATC	6 cut(s)	213, 524, 625, 758, 771, 1167
AlwNI	CAGNNNCTG	1 cut(s)	818
Ama87I	CYCGRG	1 cut(s)	213
AoxI	GGCC	4 cut(s)	141, 363, 490, 735
ApeKI	GCWGC	5 cut(s)	168, 315, 804, 910, 913
ApoI	RAATTY	3 cut(s)	30, 752, 797
Asp700I	GAANNNNTTC	2 cut(s)	853, 957
AspLEI	GCGC	2 cut(s)	826, 1033
AspS9I	GGNCC	2 cut(s)	102, 736
AsuC2I	CCSGG	2 cut(s)	99, 1181
AsuHPI	GGTGA	3 cut(s)	190, 356, 1210
AvaI	CYCGRG	1 cut(s)	213
AvaII	GGWCC	1 cut(s)	102
BamHI	GGATCC	1 cut(s)	763
BanII	GRGCYC	2 cut(s)	220, 1241
BauI	CACGAG	1 cut(s)	324
Bbv12I	GWGCWC	2 cut(s)	220, 982
BbvI	GCAGC	5 cut(s)	180, 327, 816, 922, 925
BccI	CCATC	4 cut(s)	458, 673, 715, 777
BceAI	ACGGC	2 cut(s)	176, 378
BcgI	CGANNNNNNTGC	2 cut(s)	858, 892
BciT130I	CCWGG	3 cut(s)	203, 609, 761
BcnI	CCSGG	2 cut(s)	99, 1181
BcoDI	GTCTC	1 cut(s)	881
BfaI	CTAG	1 cut(s)	236
BfmI	CTRYAG	1 cut(s)	621
BisI	GCNGC	6 cut(s)	169, 316, 597, 805, 911, 914
BlsI	GCNGC	6 cut(s)	170, 317, 598, 806, 912, 915
Bme1390I	CCNGG	5 cut(s)	99, 203, 609, 761, 1181
Bme18I	GGWCC	1 cut(s)	102
BmeT110I	CYCGRG	1 cut(s)	213
BmgT120I	GGNCC	2 cut(s)	102, 736
BmiI	GGNNCC	3 cut(s)	103, 765, 1178
BmrFI	CCNGG	5 cut(s)	99, 203, 609, 761, 1181
BmrI	ACTGGG	1 cut(s)	654
BmsI	GCATC	3 cut(s)	661, 916, 1034
BmuI	ACTGGG	1 cut(s)	654
BpmI	CTGGAG	1 cut(s)	630
BpuEI	CTTGAG	2 cut(s)	815, 905
BpuMI	CCSGG	2 cut(s)	99, 1181
BsaBI	GATNNNNATC	2 cut(s)	768, 1164
BsaHI	GRCGYC	1 cut(s)	186
BsaJI	CCNNGG	4 cut(s)	98, 147, 201, 759
BsaWI	WCCGGW	2 cut(s)	379, 513
BsaXI	ACNNNNNCTCC	2 cut(s)	606, 636
Bsc4I	CCNNNNNNNGG	5 cut(s)	241, 379, 554, 578, 614
Bse118I	RCCGGY	1 cut(s)	338
Bse1I	ACTGG	2 cut(s)	649, 1072
Bse8I	GATNNNNATC	2 cut(s)	768, 1164
BseBI	CCWGG	3 cut(s)	203, 609, 761
BseDI	CCNNGG	4 cut(s)	98, 147, 201, 759
BseGI	GGATG	4 cut(s)	250, 274, 469, 676
BseJI	GATNNNNATC	2 cut(s)	768, 1164
BseLI	CCNNNNNNNGG	5 cut(s)	241, 379, 554, 578, 614
BseMII	CTCAG	3 cut(s)	26, 396, 423
BseNI	ACTGG	2 cut(s)	649, 1072
BseRI	GAGGAG	3 cut(s)	627, 1208, 1265
BseXI	GCAGC	5 cut(s)	180, 327, 816, 922, 925
Bsh1236I	CGCG	3 cut(s)	134, 149, 1092
Bsh1285I	CGRYCG	1 cut(s)	508
BshFI	GGCC	4 cut(s)	143, 365, 492, 737
BsiEI	CGRYCG	1 cut(s)	508
BsiHKAI	GWGCWC	2 cut(s)	220, 982
BsiHKCI	CYCGRG	1 cut(s)	213
BsiSI	CCGG	5 cut(s)	98, 339, 380, 514, 1181
BslFI	GGGAC	2 cut(s)	689, 706
BslI	CCNNNNNNNGG	5 cut(s)	241, 379, 554, 578, 614
BsmAI	GTCTC	1 cut(s)	881
BsmFI	GGGAC	2 cut(s)	689, 706
BsnI	GGCC	4 cut(s)	143, 365, 492, 737
BsoBI	CYCGRG	1 cut(s)	213
Bsp1286I	GDGCHC	3 cut(s)	220, 982, 1241
Bsp143I	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
Bsp68I	TCGCGA	1 cut(s)	134
BspACI	CCGC	8 cut(s)	18, 51, 124, 147, 149, 342, 596, 1138
BspANI	GGCC	4 cut(s)	143, 365, 492, 737
BspCNI	CTCAG	3 cut(s)	27, 397, 424
BspFNI	CGCG	3 cut(s)	134, 149, 1092
BspLI	GGNNCC	3 cut(s)	103, 765, 1178
BspPI	GGATC	6 cut(s)	213, 524, 625, 758, 771, 1167
BspQI	GCTCTTC	1 cut(s)	963
BsrFI	RCCGGY	1 cut(s)	338
BsrI	ACTGG	2 cut(s)	649, 1072
BssAI	RCCGGY	1 cut(s)	338
BssECI	CCNNGG	4 cut(s)	98, 147, 201, 759
BssMI	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
BssNI	GRCGYC	1 cut(s)	186
BssSI	CACGAG	1 cut(s)	324
Bst2BI	CACGAG	1 cut(s)	324
Bst2UI	CCWGG	3 cut(s)	203, 609, 761
Bst4CI	ACNGT	3 cut(s)	506, 535, 934
Bst6I	CTCTTC	2 cut(s)	963, 1229
BstACI	GRCGYC	1 cut(s)	186
BstAPI	GCANNNNNTGC	1 cut(s)	866
BstC8I	GCNNGC	2 cut(s)	340, 1140
BstDEI	CTNAG	4 cut(s)	35, 405, 432, 718
BstDSI	CCRYGG	1 cut(s)	147
BstENI	CCTNNNNNAGG	1 cut(s)	612
BstF5I	GGATG	4 cut(s)	250, 274, 469, 676
BstFNI	CGCG	3 cut(s)	134, 149, 1092
BstHHI	GCGC	2 cut(s)	826, 1033
BstKTI	GATC	7 cut(s)	138, 208, 519, 620, 766, 1106, 1162
BstMAI	GTCTC	1 cut(s)	881
BstMBI	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
BstMCI	CGRYCG	1 cut(s)	508
BstMWI	GCNNNNNNNGC	6 cut(s)	123, 140, 224, 335, 866, 953
BstNI	CCWGG	3 cut(s)	203, 609, 761
BstSCI	CCNGG	5 cut(s)	97, 201, 607, 759, 1179
BstSFI	CTRYAG	1 cut(s)	621
BstUI	CGCG	3 cut(s)	134, 149, 1092
BstV1I	GCAGC	5 cut(s)	180, 327, 816, 922, 925
BstX2I	RGATCY	2 cut(s)	617, 763
BstXI	CCANNNNNNTGG	1 cut(s)	182
BstYI	RGATCY	2 cut(s)	617, 763
BsuRI	GGCC	4 cut(s)	143, 365, 492, 737
BtgI	CCRYGG	1 cut(s)	147
BtsCI	GGATG	4 cut(s)	250, 274, 469, 676
BtsI	GCAGTG	1 cut(s)	399
BtsIMutI	CAGTG	1 cut(s)	399
BtuMI	TCGCGA	1 cut(s)	134
Cac8I	GCNNGC	2 cut(s)	340, 1140
CaiI	CAGNNNCTG	1 cut(s)	818
CfoI	GCGC	2 cut(s)	826, 1033
Cfr10I	RCCGGY	1 cut(s)	338
Cfr13I	GGNCC	2 cut(s)	102, 736
Cfr42I	CCGCGG	1 cut(s)	150
CseI	GACGC	2 cut(s)	160, 175
Csp6I	GTAC	1 cut(s)	436
CviAII	CATG	3 cut(s)	139, 229, 460
CviQI	GTAC	1 cut(s)	436
DdeI	CTNAG	4 cut(s)	35, 405, 432, 718
DpnI	GATC	7 cut(s)	137, 207, 518, 619, 765, 1105, 1161
DpnII	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
DraI	TTTAAA	1 cut(s)	729
DrdI	GACNNNNNNGTC	1 cut(s)	158
DseDI	GACNNNNNNGTC	1 cut(s)	158
EaeI	YGGCCR	1 cut(s)	363
Eam1104I	CTCTTC	2 cut(s)	963, 1229
EarI	CTCTTC	2 cut(s)	963, 1229
EciI	GGCGGA	1 cut(s)	33
Ecl136II	GAGCTC	1 cut(s)	218
Eco24I	GRGCYC	2 cut(s)	220, 1241
Eco47I	GGWCC	1 cut(s)	102
Eco53kI	GAGCTC	1 cut(s)	218
Eco57I	CTGAAG	3 cut(s)	840, 840, 1149
Eco88I	CYCGRG	1 cut(s)	213
EcoICRI	GAGCTC	1 cut(s)	218
EcoNI	CCTNNNNNAGG	1 cut(s)	612
EcoO109I	RGGNCCY	1 cut(s)	736
EcoRII	CCWGG	3 cut(s)	201, 607, 759
EcoT38I	GRGCYC	2 cut(s)	220, 1241
FaeI	CATG	3 cut(s)	142, 232, 463
FalI	AAGNNNNNCTT	2 cut(s)	1093, 1125
FaqI	GGGAC	2 cut(s)	689, 706
FatI	CATG	3 cut(s)	138, 228, 459
FauI	CCCGC	1 cut(s)	1131
FblI	GTMKAC	1 cut(s)	746
Fnu4HI	GCNGC	6 cut(s)	169, 316, 597, 805, 911, 914
FokI	GGATG	4 cut(s)	257, 281, 476, 683
FriOI	GRGCYC	2 cut(s)	220, 1241
Fsp4HI	GCNGC	6 cut(s)	169, 316, 597, 805, 911, 914
FspBI	CTAG	1 cut(s)	236
GlaI	GCGC	2 cut(s)	825, 1032
GluI	GCNGC	6 cut(s)	169, 316, 597, 805, 911, 914
GsuI	CTGGAG	1 cut(s)	630
HaeIII	GGCC	4 cut(s)	143, 365, 492, 737
HapII	CCGG	5 cut(s)	98, 339, 380, 514, 1181
HgaI	GACGC	2 cut(s)	160, 175
HhaI	GCGC	2 cut(s)	826, 1033
Hin1I	GRCGYC	1 cut(s)	186
Hin1II	CATG	3 cut(s)	142, 232, 463
Hin6I	GCGC	2 cut(s)	824, 1031
HinP1I	GCGC	2 cut(s)	824, 1031
HindIII	AAGCTT	1 cut(s)	454
HinfI	GANTC	6 cut(s)	278, 604, 815, 835, 1165, 1201
HpaII	CCGG	5 cut(s)	98, 339, 380, 514, 1181
HphI	GGTGA	3 cut(s)	190, 356, 1210
Hpy166II	GTNNAC	3 cut(s)	175, 747, 1153
Hpy188I	TCNGA	8 cut(s)	36, 283, 433, 670, 820, 925, 1164, 1206
Hpy188III	TCNNGA	5 cut(s)	133, 266, 773, 832, 884
Hpy8I	GTNNAC	3 cut(s)	175, 747, 1153
Hpy99I	CGWCG	2 cut(s)	49, 191
HpyAV	CCTTC	3 cut(s)	128, 139, 1124
HpyCH4III	ACNGT	3 cut(s)	506, 535, 934
HpyCH4IV	ACGT	2 cut(s)	777, 1245
HpyCH4V	TGCA	4 cut(s)	423, 674, 869, 1025
HpyF10VI	GCNNNNNNNGC	6 cut(s)	123, 140, 224, 335, 866, 953
HpyF3I	CTNAG	4 cut(s)	35, 405, 432, 718
HpySE526I	ACGT	2 cut(s)	777, 1245
Hsp92I	GRCGYC	1 cut(s)	186
Hsp92II	CATG	3 cut(s)	142, 232, 463
HspAI	GCGC	2 cut(s)	824, 1031
KroI	GCCGGC	1 cut(s)	338
KroNI	GCCGGC	1 cut(s)	340
KspI	CCGCGG	1 cut(s)	150
Kzo9I	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
LguI	GCTCTTC	1 cut(s)	963
LmnI	GCTCC	2 cut(s)	20, 985
Lsp1109I	GCAGC	5 cut(s)	180, 327, 816, 922, 925
LweI	GCATC	3 cut(s)	661, 916, 1034
MaeI	CTAG	1 cut(s)	236
MaeII	ACGT	2 cut(s)	777, 1245
MaeIII	GTNAC	1 cut(s)	500
MalI	GATC	7 cut(s)	137, 207, 518, 619, 765, 1105, 1161
MboI	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
MboII	GAAGA	4 cut(s)	49, 52, 950, 1216
MfeI	CAATTG	1 cut(s)	891
MflI	RGATCY	2 cut(s)	617, 763
MhlI	GDGCHC	3 cut(s)	220, 982, 1241
MluCI	AATT	8 cut(s)	30, 73, 558, 752, 797, 891, 1006, 1052
MlyI	GAGTC	1 cut(s)	613
MroNI	GCCGGC	1 cut(s)	338
MroXI	GAANNNNTTC	2 cut(s)	853, 957
MseI	TTAA	3 cut(s)	728, 903, 1064
MslI	CAYNNNNRTG	2 cut(s)	180, 473
MspA1I	CMGCKG	2 cut(s)	149, 788
MspI	CCGG	5 cut(s)	98, 339, 380, 514, 1181
MspR9I	CCNGG	5 cut(s)	99, 203, 609, 761, 1181
MunI	CAATTG	1 cut(s)	891
MvaI	CCWGG	3 cut(s)	203, 609, 761
MvnI	CGCG	3 cut(s)	134, 149, 1092
MwoI	GCNNNNNNNGC	6 cut(s)	123, 140, 224, 335, 866, 953
NaeI	GCCGGC	1 cut(s)	340
NciI	CCSGG	2 cut(s)	99, 1181
NdeII	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
NgoMIV	GCCGGC	1 cut(s)	338
NlaIII	CATG	3 cut(s)	142, 232, 463
NlaIV	GGNNCC	3 cut(s)	103, 765, 1178
NmeAIII	GCCGAG	1 cut(s)	299
NmuCI	GTSAC	1 cut(s)	500
NruI	TCGCGA	1 cut(s)	134
PciSI	GCTCTTC	1 cut(s)	963
PdiI	GCCGGC	1 cut(s)	340
PdmI	GAANNNNTTC	2 cut(s)	853, 957
PfeI	GAWTC	5 cut(s)	278, 815, 835, 1165, 1201
PflMI	CCANNNNNTGG	2 cut(s)	554, 578
PfoI	TCCNGGA	1 cut(s)	607
PkrI	GCNGC	6 cut(s)	170, 317, 598, 806, 912, 915
PleI	GAGTC	1 cut(s)	612
PpsI	GAGTC	1 cut(s)	612
Psp124BI	GAGCTC	1 cut(s)	220
Psp1406I	AACGTT	1 cut(s)	1245
Psp6I	CCWGG	3 cut(s)	201, 607, 759
PspGI	CCWGG	3 cut(s)	201, 607, 759
PspN4I	GGNNCC	3 cut(s)	103, 765, 1178
PspPI	GGNCC	2 cut(s)	102, 736
PstNI	CAGNNNCTG	1 cut(s)	818
PsuI	RGATCY	2 cut(s)	617, 763
PvuII	CAGCTG	1 cut(s)	788
RruI	TCGCGA	1 cut(s)	134
RsaI	GTAC	1 cut(s)	437
RsaNI	GTAC	1 cut(s)	436
RseI	CAYNNNNRTG	2 cut(s)	180, 473
SacI	GAGCTC	1 cut(s)	220
SacII	CCGCGG	1 cut(s)	150
SapI	GCTCTTC	1 cut(s)	963
SaqAI	TTAA	3 cut(s)	728, 903, 1064
SatI	GCNGC	6 cut(s)	169, 316, 597, 805, 911, 914
Sau3AI	GATC	7 cut(s)	135, 205, 516, 617, 763, 1103, 1159
Sau96I	GGNCC	2 cut(s)	102, 736
SchI	GAGTC	1 cut(s)	613
ScrFI	CCNGG	5 cut(s)	99, 203, 609, 761, 1181
SduI	GDGCHC	3 cut(s)	220, 982, 1241
SfaNI	GCATC	3 cut(s)	661, 916, 1034
SfcI	CTRYAG	1 cut(s)	621
Sfr303I	CCGCGG	1 cut(s)	150
SgrBI	CCGCGG	1 cut(s)	150
SinI	GGWCC	1 cut(s)	102
SmiMI	CAYNNNNRTG	2 cut(s)	180, 473
SmlI	CTYRAG	2 cut(s)	830, 884
SmoI	CTYRAG	2 cut(s)	830, 884
Sse9I	AATT	8 cut(s)	30, 73, 558, 752, 797, 891, 1006, 1052
SsiI	CCGC	8 cut(s)	18, 51, 124, 147, 149, 342, 596, 1138
SspMI	CTAG	1 cut(s)	236
SstI	GAGCTC	1 cut(s)	220
StyD4I	CCNGG	5 cut(s)	97, 201, 607, 759, 1179
TaaI	ACNGT	3 cut(s)	506, 535, 934
TaiI	ACGT	2 cut(s)	780, 1248
TaqI	TCGA	2 cut(s)	847, 1227
TasI	AATT	8 cut(s)	30, 73, 558, 752, 797, 891, 1006, 1052
TatI	WGTACW	1 cut(s)	435
TauI	GCSGC	1 cut(s)	599
TfiI	GAWTC	5 cut(s)	278, 815, 835, 1165, 1201
Tru1I	TTAA	3 cut(s)	728, 903, 1064
Tru9I	TTAA	3 cut(s)	728, 903, 1064
TscAI	CASTG	1 cut(s)	406
TseFI	GTSAC	1 cut(s)	500
TseI	GCWGC	5 cut(s)	168, 315, 804, 910, 913
Tsp45I	GTSAC	1 cut(s)	500
TspDTI	ATGAA	6 cut(s)	245, 285, 448, 912, 966, 1091
TspRI	CASTG	1 cut(s)	406
Van91I	CCANNNNNTGG	2 cut(s)	554, 578
VpaK11BI	GGWCC	1 cut(s)	102
XagI	CCTNNNNNAGG	1 cut(s)	612
XapI	RAATTY	3 cut(s)	30, 752, 797
XmiI	GTMKAC	1 cut(s)	746
XmnI	GAANNNNTTC	2 cut(s)	853, 957
XspI	CTAG	1 cut(s)	236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.