Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003536

Physical Location & Seq

Genomic Coordinates Forward (+)

14947755 .. 14951714

Gene Structure 3960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1189500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGCGAATCTTATTCGGACGAGCGACCCAAATTCTGAGCAAATCGTCTTCCGCTTTCTTCTTCTTCCTCAATCCAAATTCCAAACTCTCTCTGTGCAAACTCTCCGAATCCCCTCTCCTTCTCCGAACCTTCGCGATCATGGCCTCCGCCGACGAGTTCGTCAAGGGCAGTGTCCACCCGAACGGCGTCGCAGTCATCACCCTCGATCGCCCCCGAGCTCTCAACGCCATGAACCTAGATATGGATGTGAAATACAAAAGCTATCTGGATGAATGGGAATCAGACCCGAGTGTGAAGTGTGTTCTTGTCGATAGCAGCTCGGCCCGTGCCTTTTGTGCTGGAATGGATATTAAAGGGGTTGTTGCCGAAATTCAAAAGGACAAAAACACACCTCTTGTGCCGAAGGTATTCACTGCTGAGTATTCTCTAATATGTAAAATATCTGAGTACAAGAAGCCCTATATAAGCTTCATGGATGGCATAACAATGGGCTTTGGTATTGGCCTATCAGGTCATGGTCGCTACCGGATCATTACAGAGAGAACTGTTCTTGCTATGCCAGAGAATGGAATTGGTTTGTTTCCAGATGTTGGGTTTTCACATATTGCGGCAAAGAGTCCAGGAGGAGGATCTGTAGGTGCTTATCTTGGACTGACTGGTAAAAGGATTTCAACACCATCGGATGCACTATATGTGGGTCTTGGGACACATTTTGTCCCATCTCAAAACTTAGGTTCTCTAAAGGAGGCCCTTTTGTCAACCAACTTTTCTCAGGATCCACATCAGGACATCGAAACAAATTTGACGAAATTCAGCAGCAACCCAGATTCTGAACCCCAACTTAAGTCGCTGCTGCCTCCAATAACTTCCACATTTGGTGCAAATAAGTCAGTTGTCGAGACAATTGATGAACTTAAAAAGCAGCAGCAGAGTTCAGATGCTACTGTGGTGGAGTGGGCTAATGAAGCTCTTCAAGGTATTGGAAAGGGTGCTCCTTTTTCACTTTACCTGACACAAAATTATTTCTCCAAGGTTGCATCTGCGCTTGGGAAAAATGAGAATCAATTATCCACATTAACAGGTGTGATGAAAACTGAATATCGCATTGCAATAAGATCGTCTTTGCGGAGTGATTTTGCTGAAGGTGTGCGCGCAGTTTTGGTGGACAAGGATCAGAATCCAAAGTGGAACCCGTCAAAGTTAGAAGAGGTTAATCAAAGCGAAGTTGAAGCTCTATTCGAGCCATTGAGCCCGAACGTTGAGGAGTTGAGCGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0016020 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

426

Amino Acids

46.3

Weight (kDa)

6.06

Isoelectric Point (pI)

31.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	60 - 246	1.3e-23	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	64 - 413	1.8e-105	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003536)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24360 AT3G24360 AT3G24360 AT3G24360 AT3G24360 AT3G24360 AT4G13360
fragaria_vesca	FvH4_1g05350 FvH4_1g05350 FvH4_1g05350 FvH4_1g05350 FvH4_1g05350 FvH4_1g05350
malus_domestica	MD02G1052700.v1.1 MD02G1056500.v1.1 MD15G1189500.v1.1
prunus_persica	Prupe.7G227300_v2.0.a1
pyrus_communis	pycom02g04570 pycom15g16990
rosa_chinensis	RchiOBHm_Chr2g0091031 RchiOBHm_Chr2g0092761 RchiOBHm_Chr2g0092831
rosa_laevigata	RLG00000016169
rosa_multiflora	Rmu_sc0003305.1_g000013
rosa_roxburghii	Rroxscaffold_2G00148870 Rroxscaffold_2G00150670
rosa_rugosa	Rorug02G0011500 Rorug02G0011500 Rorug02G0011600 Rorug02G0029300
rosa_samantha	Rh2AG058000 Rh2BG056300 Rh2CG058600 Rh2DG057800
rosa_wichuraiana	Rw2G005120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	158
AccB7I	CCANNNNNTGG	2 cut(s)	566, 590
AccII	CGCG	2 cut(s)	134, 1152
AciI	CCGC	4 cut(s)	51, 147, 608, 1126
AclI	AACGTT	1 cut(s)	1257
AclWI	GGATC	5 cut(s)	536, 637, 770, 783, 1179
AcsI	RAATTY	5 cut(s)	30, 76, 369, 799, 809
AcuI	CTGAAG	1 cut(s)	1161
AcyI	GRCGYC	1 cut(s)	186
AfaI	GTAC	1 cut(s)	449
AfiI	CCNNNNNNNGG	4 cut(s)	241, 566, 590, 626
AflII	CTTAAG	1 cut(s)	842
AgsI	TTSAA	4 cut(s)	374, 672, 974, 1229
AjnI	CCWGG	1 cut(s)	619
AluBI	AGCT	6 cut(s)	218, 261, 318, 468, 968, 1232
AluI	AGCT	6 cut(s)	218, 261, 318, 468, 968, 1232
Alw21I	GWGCWC	2 cut(s)	220, 994
Alw26I	GTCTC	1 cut(s)	893
AlwI	GGATC	5 cut(s)	536, 637, 770, 783, 1179
AlwNI	CAGNNNCTG	1 cut(s)	830
Ama87I	CYCGRG	2 cut(s)	213, 286
AoxI	GGCC	4 cut(s)	141, 321, 502, 747
ApeKI	GCWGC	6 cut(s)	315, 816, 850, 853, 922, 925
ApoI	RAATTY	5 cut(s)	30, 76, 369, 799, 809
Asp700I	GAANNNNTTC	2 cut(s)	407, 969
AspLEI	GCGC	3 cut(s)	1045, 1152, 1154
AspS9I	GGNCC	2 cut(s)	322, 748
AsuHPI	GGTGA	1 cut(s)	190
AvaI	CYCGRG	2 cut(s)	213, 286
BamHI	GGATCC	1 cut(s)	775
BanII	GRGCYC	2 cut(s)	220, 1253
BbsI	GAAGAC	1 cut(s)	39
Bbv12I	GWGCWC	2 cut(s)	220, 994
BbvI	GCAGC	6 cut(s)	327, 828, 837, 840, 934, 937
BccI	CCATC	3 cut(s)	470, 685, 727
BceAI	ACGGC	1 cut(s)	199
BcgI	CGANNNNNNTGC	2 cut(s)	1098, 1132
BciT130I	CCWGG	1 cut(s)	621
BcoDI	GTCTC	1 cut(s)	893
BfaI	CTAG	1 cut(s)	236
BfmI	CTRYAG	1 cut(s)	633
BfrI	CTTAAG	1 cut(s)	842
BisI	GCNGC	7 cut(s)	316, 609, 817, 851, 854, 923, 926
BlsI	GCNGC	7 cut(s)	317, 610, 818, 852, 855, 924, 927
Bme1390I	CCNGG	1 cut(s)	621
BmeT110I	CYCGRG	2 cut(s)	213, 286
BmgT120I	GGNCC	2 cut(s)	322, 748
BmiI	GGNNCC	2 cut(s)	777, 1190
BmrFI	CCNGG	1 cut(s)	621
BmsI	GCATC	3 cut(s)	673, 928, 1046
BpiI	GAAGAC	1 cut(s)	39
BsaBI	GATNNNNATC	2 cut(s)	780, 1176
BsaHI	GRCGYC	1 cut(s)	186
BsaJI	CCNNGG	1 cut(s)	1029
BsaWI	WCCGGW	1 cut(s)	525
BsaXI	ACNNNNNCTCC	2 cut(s)	618, 648
Bsc4I	CCNNNNNNNGG	4 cut(s)	241, 566, 590, 626
Bse1I	ACTGG	1 cut(s)	661
Bse3DI	GCAATG	1 cut(s)	1104
Bse8I	GATNNNNATC	2 cut(s)	780, 1176
BseBI	CCWGG	1 cut(s)	621
BseDI	CCNNGG	1 cut(s)	1029
BseGI	GGATG	4 cut(s)	250, 274, 481, 688
BseJI	GATNNNNATC	2 cut(s)	780, 1176
BseLI	CCNNNNNNNGG	4 cut(s)	241, 566, 590, 626
BseMI	GCAATG	1 cut(s)	1104
BseMII	CTCAG	4 cut(s)	26, 408, 435, 785
BseNI	ACTGG	1 cut(s)	661
BsePI	GCGCGC	1 cut(s)	1150
BseRI	GAGGAG	2 cut(s)	639, 1277
BseXI	GCAGC	6 cut(s)	327, 828, 837, 840, 934, 937
Bsh1236I	CGCG	2 cut(s)	134, 1152
Bsh1285I	CGRYCG	1 cut(s)	208
BshFI	GGCC	4 cut(s)	143, 323, 504, 749
BsiEI	CGRYCG	1 cut(s)	208
BsiHKAI	GWGCWC	2 cut(s)	220, 994
BsiHKCI	CYCGRG	2 cut(s)	213, 286
BsiSI	CCGG	1 cut(s)	526
BslFI	GGGAC	2 cut(s)	701, 718
BslI	CCNNNNNNNGG	4 cut(s)	241, 566, 590, 626
BsmAI	GTCTC	1 cut(s)	893
BsmFI	GGGAC	2 cut(s)	701, 718
BsnI	GGCC	4 cut(s)	143, 323, 504, 749
BsoBI	CYCGRG	2 cut(s)	213, 286
Bsp1286I	GDGCHC	3 cut(s)	220, 994, 1253
Bsp143I	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
Bsp68I	TCGCGA	1 cut(s)	134
BspACI	CCGC	4 cut(s)	51, 147, 608, 1126
BspANI	GGCC	4 cut(s)	143, 323, 504, 749
BspCNI	CTCAG	4 cut(s)	27, 409, 436, 784
BspFNI	CGCG	2 cut(s)	134, 1152
BspLI	GGNNCC	2 cut(s)	777, 1190
BspPI	GGATC	5 cut(s)	536, 637, 770, 783, 1179
BspQI	GCTCTTC	1 cut(s)	975
BspTI	CTTAAG	1 cut(s)	842
BsrDI	GCAATG	1 cut(s)	1104
BsrI	ACTGG	1 cut(s)	661
BssECI	CCNNGG	1 cut(s)	1029
BssHII	GCGCGC	1 cut(s)	1150
BssMI	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
BssNI	GRCGYC	1 cut(s)	186
BssT1I	CCWWGG	1 cut(s)	1029
Bst2UI	CCWGG	1 cut(s)	621
Bst4CI	ACNGT	2 cut(s)	547, 946
Bst6I	CTCTTC	2 cut(s)	975, 1200
BstACI	GRCGYC	1 cut(s)	186
BstAFI	CTTAAG	1 cut(s)	842
BstC8I	GCNNGC	1 cut(s)	1152
BstDEI	CTNAG	5 cut(s)	35, 417, 444, 730, 771
BstF5I	GGATG	4 cut(s)	250, 274, 481, 688
BstFNI	CGCG	2 cut(s)	134, 1152
BstHHI	GCGC	3 cut(s)	1045, 1152, 1154
BstKTI	GATC	7 cut(s)	138, 208, 531, 632, 778, 1118, 1174
BstMAI	GTCTC	1 cut(s)	893
BstMBI	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
BstMCI	CGRYCG	1 cut(s)	208
BstMWI	GCNNNNNNNGC	4 cut(s)	140, 224, 335, 965
BstNI	CCWGG	1 cut(s)	621
BstSCI	CCNGG	1 cut(s)	619
BstSFI	CTRYAG	1 cut(s)	633
BstUI	CGCG	2 cut(s)	134, 1152
BstV1I	GCAGC	6 cut(s)	327, 828, 837, 840, 934, 937
BstV2I	GAAGAC	1 cut(s)	39
BstX2I	RGATCY	2 cut(s)	629, 775
BstYI	RGATCY	2 cut(s)	629, 775
BsuRI	GGCC	4 cut(s)	143, 323, 504, 749
BtsCI	GGATG	4 cut(s)	250, 274, 481, 688
BtsI	GCAGTG	2 cut(s)	175, 411
BtsIMutI	CAGTG	2 cut(s)	175, 411
BtuMI	TCGCGA	1 cut(s)	134
Cac8I	GCNNGC	1 cut(s)	1152
CaiI	CAGNNNCTG	1 cut(s)	830
CfoI	GCGC	3 cut(s)	1045, 1152, 1154
Cfr13I	GGNCC	2 cut(s)	322, 748
CseI	GACGC	1 cut(s)	175
Csp6I	GTAC	1 cut(s)	448
CviAII	CATG	4 cut(s)	139, 229, 472, 515
CviQI	GTAC	1 cut(s)	448
DdeI	CTNAG	5 cut(s)	35, 417, 444, 730, 771
DpnI	GATC	7 cut(s)	137, 207, 530, 631, 777, 1117, 1173
DpnII	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
DrdI	GACNNNNNNGTC	1 cut(s)	158
DseDI	GACNNNNNNGTC	1 cut(s)	158
Eam1104I	CTCTTC	2 cut(s)	975, 1200
EarI	CTCTTC	2 cut(s)	975, 1200
EciI	GGCGGA	1 cut(s)	136
Ecl136II	GAGCTC	1 cut(s)	218
Eco130I	CCWWGG	1 cut(s)	1029
Eco24I	GRGCYC	2 cut(s)	220, 1253
Eco53kI	GAGCTC	1 cut(s)	218
Eco57I	CTGAAG	1 cut(s)	1161
Eco88I	CYCGRG	2 cut(s)	213, 286
EcoICRI	GAGCTC	1 cut(s)	218
EcoO109I	RGGNCCY	1 cut(s)	748
EcoRII	CCWGG	1 cut(s)	619
EcoT14I	CCWWGG	1 cut(s)	1029
EcoT38I	GRGCYC	2 cut(s)	220, 1253
ErhI	CCWWGG	1 cut(s)	1029
FaeI	CATG	4 cut(s)	142, 232, 475, 518
FalI	AAGNNNNNCTT	2 cut(s)	1105, 1137
FaqI	GGGAC	2 cut(s)	701, 718
FatI	CATG	4 cut(s)	138, 228, 471, 514
Fnu4HI	GCNGC	7 cut(s)	316, 609, 817, 851, 854, 923, 926
FokI	GGATG	4 cut(s)	257, 281, 488, 695
FriOI	GRGCYC	2 cut(s)	220, 1253
Fsp4HI	GCNGC	7 cut(s)	316, 609, 817, 851, 854, 923, 926
FspBI	CTAG	1 cut(s)	236
GlaI	GCGC	3 cut(s)	1044, 1151, 1153
GluI	GCNGC	7 cut(s)	316, 609, 817, 851, 854, 923, 926
HaeIII	GGCC	4 cut(s)	143, 323, 504, 749
HapII	CCGG	1 cut(s)	526
HgaI	GACGC	1 cut(s)	175
HhaI	GCGC	3 cut(s)	1045, 1152, 1154
Hin1I	GRCGYC	1 cut(s)	186
Hin1II	CATG	4 cut(s)	142, 232, 475, 518
Hin6I	GCGC	3 cut(s)	1043, 1150, 1152
HinP1I	GCGC	3 cut(s)	1043, 1150, 1152
HincII	GTYRAC	1 cut(s)	759
HindII	GTYRAC	1 cut(s)	759
HindIII	AAGCTT	1 cut(s)	466
HinfI	GANTC	7 cut(s)	6, 107, 278, 616, 827, 1060, 1177
HpaII	CCGG	1 cut(s)	526
HphI	GGTGA	1 cut(s)	190
Hpy166II	GTNNAC	3 cut(s)	175, 759, 1165
Hpy188III	TCNNGA	6 cut(s)	133, 266, 584, 773, 785, 898
Hpy8I	GTNNAC	3 cut(s)	175, 759, 1165
Hpy99I	CGWCG	2 cut(s)	155, 191
HpyAV	CCTTC	4 cut(s)	128, 139, 397, 1136
HpyCH4III	ACNGT	2 cut(s)	547, 946
HpyCH4IV	ACGT	1 cut(s)	1257
HpyCH4V	TGCA	5 cut(s)	96, 686, 881, 1037, 1109
HpyF10VI	GCNNNNNNNGC	4 cut(s)	140, 224, 335, 965
HpyF3I	CTNAG	5 cut(s)	35, 417, 444, 730, 771
HpySE526I	ACGT	1 cut(s)	1257
Hsp92I	GRCGYC	1 cut(s)	186
Hsp92II	CATG	4 cut(s)	142, 232, 475, 518
HspAI	GCGC	3 cut(s)	1043, 1150, 1152
Kzo9I	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
LguI	GCTCTTC	1 cut(s)	975
LmnI	GCTCC	1 cut(s)	997
Lsp1109I	GCAGC	6 cut(s)	327, 828, 837, 840, 934, 937
LweI	GCATC	3 cut(s)	673, 928, 1046
MaeI	CTAG	1 cut(s)	236
MaeII	ACGT	1 cut(s)	1257
MalI	GATC	7 cut(s)	137, 207, 530, 631, 777, 1117, 1173
MboI	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
MboII	GAAGA	6 cut(s)	39, 49, 52, 55, 962, 1217
MfeI	CAATTG	1 cut(s)	903
MflI	RGATCY	2 cut(s)	629, 775
MhlI	GDGCHC	3 cut(s)	220, 994, 1253
MluCI	AATT	9 cut(s)	30, 76, 369, 570, 799, 809, 903, 1018, 1064
MlyI	GAGTC	1 cut(s)	625
MroXI	GAANNNNTTC	2 cut(s)	407, 969
MseI	TTAA	5 cut(s)	351, 843, 915, 1076, 1212
MslI	CAYNNNNRTG	1 cut(s)	485
MspCI	CTTAAG	1 cut(s)	842
MspI	CCGG	1 cut(s)	526
MspR9I	CCNGG	1 cut(s)	621
MunI	CAATTG	1 cut(s)	903
MvaI	CCWGG	1 cut(s)	621
MvnI	CGCG	2 cut(s)	134, 1152
MwoI	GCNNNNNNNGC	4 cut(s)	140, 224, 335, 965
NdeII	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
NlaIII	CATG	4 cut(s)	142, 232, 475, 518
NlaIV	GGNNCC	2 cut(s)	777, 1190
NmeAIII	GCCGAG	1 cut(s)	299
NruI	TCGCGA	1 cut(s)	134
PauI	GCGCGC	1 cut(s)	1150
PciSI	GCTCTTC	1 cut(s)	975
PdmI	GAANNNNTTC	2 cut(s)	407, 969
PfeI	GAWTC	6 cut(s)	6, 107, 278, 827, 1060, 1177
PflMI	CCANNNNNTGG	2 cut(s)	566, 590
PfoI	TCCNGGA	1 cut(s)	619
PkrI	GCNGC	7 cut(s)	317, 610, 818, 852, 855, 924, 927
Ple19I	CGATCG	1 cut(s)	208
PleI	GAGTC	1 cut(s)	624
PpsI	GAGTC	1 cut(s)	624
Psp124BI	GAGCTC	1 cut(s)	220
Psp1406I	AACGTT	1 cut(s)	1257
Psp6I	CCWGG	1 cut(s)	619
PspGI	CCWGG	1 cut(s)	619
PspN4I	GGNNCC	2 cut(s)	777, 1190
PspPI	GGNCC	2 cut(s)	322, 748
PstNI	CAGNNNCTG	1 cut(s)	830
PsuI	RGATCY	2 cut(s)	629, 775
PteI	GCGCGC	1 cut(s)	1150
PvuI	CGATCG	1 cut(s)	208
RruI	TCGCGA	1 cut(s)	134
RsaI	GTAC	1 cut(s)	449
RsaNI	GTAC	1 cut(s)	448
RseI	CAYNNNNRTG	1 cut(s)	485
SacI	GAGCTC	1 cut(s)	220
SapI	GCTCTTC	1 cut(s)	975
SaqAI	TTAA	5 cut(s)	351, 843, 915, 1076, 1212
SatI	GCNGC	7 cut(s)	316, 609, 817, 851, 854, 923, 926
Sau3AI	GATC	7 cut(s)	135, 205, 528, 629, 775, 1115, 1171
Sau96I	GGNCC	2 cut(s)	322, 748
SchI	GAGTC	1 cut(s)	625
ScrFI	CCNGG	1 cut(s)	621
SduI	GDGCHC	3 cut(s)	220, 994, 1253
SfaNI	GCATC	3 cut(s)	673, 928, 1046
SfcI	CTRYAG	1 cut(s)	633
SmiMI	CAYNNNNRTG	1 cut(s)	485
SmlI	CTYRAG	1 cut(s)	842
SmoI	CTYRAG	1 cut(s)	842
Sse9I	AATT	9 cut(s)	30, 76, 369, 570, 799, 809, 903, 1018, 1064
SsiI	CCGC	4 cut(s)	51, 147, 608, 1126
SspMI	CTAG	1 cut(s)	236
SstI	GAGCTC	1 cut(s)	220
StyD4I	CCNGG	1 cut(s)	619
StyI	CCWWGG	1 cut(s)	1029
TaaI	ACNGT	2 cut(s)	547, 946
TaiI	ACGT	1 cut(s)	1260
TaqI	TCGA	5 cut(s)	204, 309, 792, 897, 1239
TasI	AATT	9 cut(s)	30, 76, 369, 570, 799, 809, 903, 1018, 1064
TatI	WGTACW	1 cut(s)	447
TauI	GCSGC	1 cut(s)	611
TfiI	GAWTC	6 cut(s)	6, 107, 278, 827, 1060, 1177
Tru1I	TTAA	5 cut(s)	351, 843, 915, 1076, 1212
Tru9I	TTAA	5 cut(s)	351, 843, 915, 1076, 1212
TscAI	CASTG	2 cut(s)	175, 418
TseI	GCWGC	6 cut(s)	315, 816, 850, 853, 922, 925
TspDTI	ATGAA	6 cut(s)	245, 285, 460, 924, 978, 1103
TspRI	CASTG	2 cut(s)	175, 418
Van91I	CCANNNNNTGG	2 cut(s)	566, 590
Vha464I	CTTAAG	1 cut(s)	842
XapI	RAATTY	5 cut(s)	30, 76, 369, 799, 809
XmnI	GAANNNNTTC	2 cut(s)	407, 969
XspI	CTAG	1 cut(s)	236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.