FvH4_1g05350

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003536
Physical Location & Seq
Forward (+)
2829241 .. 2833550
4310 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05350.t3

Sequence Viewer

Length: 1245 bp
ATGCGAAGCTTATTCGGAGCGACCCAGATTCTGAGCAAGTCCTCATCTTTCTTCCTACCCACAAATCCCAACCTCTCCCTCTCCAAAACCCACATCTTCCTTCGAACCTTCGCCACCATGGCCGCCGCCGACGAGTTCGTCAAGGGCTCCGTCCACCCAAACGGCGTCGCTTTGATCACCCTCGATCGCCCCAAAGCTCTCAACGCCATGAACATAGATATGGATTTGAAGTACAAGAGCTATCTGGATCAGTGGGAATCCGACCCGAATGTGAAGTGTGTTTTGGTGGATAGCAGCTCAGCTCGTGCCTTTTGTGCTGGCGGTGATGTGAAGCAGATCACGGCCAAAAATCAACTATCCGATATGATTGAGGTATTCACTGCGGAGTATTCTTTAATATGTAAAATATCTGACTACAAGAAGCCGTATGTAAGTTTCATGGATGGTGTAACAATGGGCTTTGGAATTGGCCTGTCAGGTCATGGTCGCTACCGGATCATTACAGAGAGGACTCTTCTTGCTATGCCAGAAAATGGTATTGGATTGTTTCCAGACGTTGGCTTTTCACATATAGCGGCACATTGTCCAGGAGGAGGATCAGTTGGTGCTTACCTTGGAATCACCGGGAAAAGAATTTCGACTCCATCGGATGCACTATATGTAGGTCTTGGGACACATTTTGTTCCATCTCAAAACTTAGGTTCAGTAAAGGAGGCCCTTTTGTCAACTAACTTTGGTCAGGATCCACATGAGGACATCAAAACAATTTTGGCGAAGTTCAGCAGTAACCCTGATTCTGAATCCCAACTAAAGCAGCTTTTACCTGAAATCACCTCATCATTTGGTGCAAATAAGTCAGTTATTGAGACAATTGAAGAACTTAAAAAGCATCAATCGAGTTCAGATGCTACTGTGGTGGAGTGGGCTAATGAAGCTCTTCAAGGTATTGGAAAGGGTGCTCCTTTTTCACTTTTCTTGACACAGAAGTATTTCTCCAAAGTTGCATCTGCCCTTGGGAAGAATGACGATCAATTTTCGACACTGACTGGCGTCATGAAAACTGAATACCGCATTGCCTTAAGATCATCCCTGCGTAATGACTTTGCTGAAGGTGTGCGTGCAGTGTTGGTGGACAAGGATCAGAATCCGAAGTGGAACCCGTCACGGTTGGAGGAAGTTAATGAGAGCGAAGTGGAAGCTCTGTTCGAACCATTGAGCCCAAATGTGGAGGAGTTGAGTGTGTAA

Protein Analysis

415

Amino Acids

45.09

Weight (kDa)

5.7

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 1 - 256 4.5e-70 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 137
AccB7I CCANNNNNTGG 2 cut(s) 533, 557
AciI CCGC 6 cut(s) 123, 126, 321, 383, 575, 1069
AclWI GGATC 6 cut(s) 255, 503, 604, 737, 750, 1146
AcoI YGGCCR 2 cut(s) 120, 342
AcsI RAATTY 1 cut(s) 633
AcuI CTGAAG 1 cut(s) 1128
AcyI GRCGYC 2 cut(s) 165, 1050
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 4 cut(s) 533, 557, 593, 1225
AflII CTTAAG 1 cut(s) 1078
AgsI TTSAA 3 cut(s) 229, 875, 941
AjnI CCWGG 1 cut(s) 586
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 522, 554
AloI GAACNNNNNNTCC 2 cut(s) 1187, 1219
AluBI AGCT 8 cut(s) 9, 197, 240, 297, 302, 817, 935, 1199
AluI AGCT 8 cut(s) 9, 197, 240, 297, 302, 817, 935, 1199
Alw21I GWGCWC 1 cut(s) 961
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 6 cut(s) 255, 503, 604, 737, 750, 1146
AlwNI CAGNNNCTG 1 cut(s) 31
AoxI GGCC 4 cut(s) 120, 342, 469, 714
ApeKI GCWGC 2 cut(s) 294, 814
ApoI RAATTY 1 cut(s) 633
Asp700I GAANNNNTTC 2 cut(s) 936, 989
AspS9I GGNCC 1 cut(s) 715
AsuC2I CCSGG 1 cut(s) 625
AsuHPI GGTGA 4 cut(s) 169, 335, 613, 823
AsuII TTCGAA 2 cut(s) 103, 1206
BamHI GGATCC 1 cut(s) 742
BanII GRGCYC 2 cut(s) 149, 1220
BauI CACGAG 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 961
BbvI GCAGC 2 cut(s) 306, 826
BccI CCATC 3 cut(s) 437, 652, 694
BceAI ACGGC 3 cut(s) 178, 357, 409
BciT130I CCWGG 1 cut(s) 588
BclI TGATCA 1 cut(s) 174
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 1 cut(s) 860
BfrI CTTAAG 1 cut(s) 1078
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 5 cut(s) 123, 126, 295, 576, 815
BlpI GCTNAGC 1 cut(s) 298
BlsI GCNGC 5 cut(s) 124, 127, 296, 577, 816
Bme1390I CCNGG 2 cut(s) 588, 625
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 3 cut(s) 148, 744, 1157
BmrFI CCNGG 2 cut(s) 588, 625
BmsI GCATC 4 cut(s) 640, 895, 898, 1013
BoxI GACNNNNGTC 1 cut(s) 1049
Bpu1102I GCTNAGC 1 cut(s) 298
Bpu14I TTCGAA 2 cut(s) 103, 1206
BpuMI CCSGG 1 cut(s) 625
BsaBI GATNNNNATC 1 cut(s) 1143
BsaHI GRCGYC 2 cut(s) 165, 1050
BsaJI CCNNGG 3 cut(s) 117, 613, 1012
BsaWI WCCGGW 1 cut(s) 492
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 4 cut(s) 533, 557, 593, 1225
Bse1I ACTGG 1 cut(s) 1051
Bse3DI GCAATG 1 cut(s) 1071
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 1 cut(s) 588
BseDI CCNNGG 3 cut(s) 117, 613, 1012
BseGI GGATG 3 cut(s) 448, 655, 1085
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 4 cut(s) 533, 557, 593, 1225
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 2 cut(s) 23, 312
BseNI ACTGG 1 cut(s) 1051
BseRI GAGGAG 2 cut(s) 606, 1244
BseXI GCAGC 2 cut(s) 306, 826
BsgI GTGCAG 1 cut(s) 1140
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 4 cut(s) 122, 344, 471, 716
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 961
BsiSI CCGG 2 cut(s) 493, 624
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 4 cut(s) 533, 557, 593, 1225
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 1 cut(s) 685
BsnI GGCC 4 cut(s) 122, 344, 471, 716
Bsp119I TTCGAA 2 cut(s) 103, 1206
Bsp1286I GDGCHC 3 cut(s) 149, 961, 1220
Bsp1720I GCTNAGC 1 cut(s) 298
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 6 cut(s) 123, 126, 321, 383, 575, 1069
BspANI GGCC 4 cut(s) 122, 344, 471, 716
BspCNI CTCAG 2 cut(s) 24, 311
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 3 cut(s) 148, 744, 1157
BspPI GGATC 6 cut(s) 255, 503, 604, 737, 750, 1146
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 2 cut(s) 103, 1206
BspTI CTTAAG 1 cut(s) 1078
BsrDI GCAATG 1 cut(s) 1071
BsrI ACTGG 1 cut(s) 1051
BssECI CCNNGG 3 cut(s) 117, 613, 1012
BssNI GRCGYC 2 cut(s) 165, 1050
BssSI CACGAG 1 cut(s) 303
BssT1I CCWWGG 3 cut(s) 117, 613, 1012
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 588
Bst4CI ACNGT 2 cut(s) 913, 1167
Bst6I CTCTTC 2 cut(s) 519, 942
BstACI GRCGYC 2 cut(s) 165, 1050
BstAFI CTTAAG 1 cut(s) 1078
BstBI TTCGAA 2 cut(s) 103, 1206
BstC8I GCNNGC 2 cut(s) 319, 1119
BstDEI CTNAG 3 cut(s) 32, 298, 697
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 3 cut(s) 448, 655, 1085
BstMAI GTCTC 1 cut(s) 860
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 4 cut(s) 119, 203, 314, 932
BstNI CCWGG 1 cut(s) 588
BstPAI GACNNNNGTC 1 cut(s) 1049
BstSCI CCNGG 2 cut(s) 586, 623
BstV1I GCAGC 2 cut(s) 306, 826
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 4 cut(s) 122, 344, 471, 716
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 3 cut(s) 448, 655, 1085
BtsI GCAGTG 2 cut(s) 378, 1128
BtsIMutI CAGTG 4 cut(s) 257, 378, 1040, 1128
Cac8I GCNNGC 2 cut(s) 319, 1119
CaiI CAGNNNCTG 1 cut(s) 31
CciI TCATGA 1 cut(s) 1053
Cfr13I GGNCC 1 cut(s) 715
CseI GACGC 2 cut(s) 154, 1039
Csp6I GTAC 1 cut(s) 232
CviAII CATG 6 cut(s) 118, 208, 439, 482, 749, 1054
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 3 cut(s) 32, 298, 697
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
EaeI YGGCCR 2 cut(s) 120, 342
Eam1104I CTCTTC 2 cut(s) 519, 942
EarI CTCTTC 2 cut(s) 519, 942
Eco130I CCWWGG 3 cut(s) 117, 613, 1012
Eco24I GRGCYC 2 cut(s) 149, 1220
Eco57I CTGAAG 1 cut(s) 1128
EcoO109I RGGNCCY 1 cut(s) 715
EcoRII CCWGG 1 cut(s) 586
EcoT14I CCWWGG 3 cut(s) 117, 613, 1012
EcoT38I GRGCYC 2 cut(s) 149, 1220
ErhI CCWWGG 3 cut(s) 117, 613, 1012
FaeI CATG 6 cut(s) 121, 211, 442, 485, 752, 1057
FaqI GGGAC 1 cut(s) 685
FatI CATG 6 cut(s) 117, 207, 438, 481, 748, 1053
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 5 cut(s) 123, 126, 295, 576, 815
FokI GGATG 3 cut(s) 455, 662, 1072
FriOI GRGCYC 2 cut(s) 149, 1220
Fsp4HI GCNGC 5 cut(s) 123, 126, 295, 576, 815
GluI GCNGC 5 cut(s) 123, 126, 295, 576, 815
HaeIII GGCC 4 cut(s) 122, 344, 471, 716
HapII CCGG 2 cut(s) 493, 624
HgaI GACGC 2 cut(s) 154, 1039
Hin1I GRCGYC 2 cut(s) 165, 1050
Hin1II CATG 6 cut(s) 121, 211, 442, 485, 752, 1057
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 8 cut(s) 28, 257, 511, 618, 640, 794, 800, 1144
HpaII CCGG 2 cut(s) 493, 624
HphI GGTGA 4 cut(s) 169, 335, 613, 823
Hpy166II GTNNAC 3 cut(s) 154, 726, 1132
Hpy188III TCNNGA 5 cut(s) 245, 551, 740, 976, 1054
Hpy8I GTNNAC 3 cut(s) 154, 726, 1132
Hpy99I CGWCG 2 cut(s) 134, 170
HpyAV CCTTC 3 cut(s) 110, 118, 1103
HpyCH4III ACNGT 2 cut(s) 913, 1167
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 4 cut(s) 653, 848, 1004, 1121
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 203, 314, 932
HpyF3I CTNAG 3 cut(s) 32, 298, 697
HpySE526I ACGT 1 cut(s) 555
Hsp92I GRCGYC 2 cut(s) 165, 1050
Hsp92II CATG 6 cut(s) 121, 211, 442, 485, 752, 1057
Ksp22I TGATCA 1 cut(s) 174
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 3 cut(s) 17, 152, 964
Lsp1109I GCAGC 2 cut(s) 306, 826
LweI GCATC 4 cut(s) 640, 895, 898, 1013
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 3 cut(s) 448, 785, 1161
MboII GAAGA 6 cut(s) 43, 88, 506, 887, 929, 1030
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 3 cut(s) 149, 961, 1220
MluCI AATT 5 cut(s) 465, 633, 765, 870, 1031
MlyI GAGTC 2 cut(s) 505, 634
MmeI TCCRAC 2 cut(s) 285, 1149
MroXI GAANNNNTTC 2 cut(s) 936, 989
MseI TTAA 4 cut(s) 395, 882, 1079, 1179
MslI CAYNNNNRTG 1 cut(s) 218
MspCI CTTAAG 1 cut(s) 1078
MspI CCGG 2 cut(s) 493, 624
MspR9I CCNGG 2 cut(s) 588, 625
MunI CAATTG 1 cut(s) 870
MvaI CCWGG 1 cut(s) 588
MwoI GCNNNNNNNGC 4 cut(s) 119, 203, 314, 932
NciI CCSGG 1 cut(s) 625
NcoI CCATGG 1 cut(s) 117
NlaIII CATG 6 cut(s) 121, 211, 442, 485, 752, 1057
NlaIV GGNNCC 3 cut(s) 148, 744, 1157
NmuCI GTSAC 1 cut(s) 1161
NspV TTCGAA 2 cut(s) 103, 1206
PagI TCATGA 1 cut(s) 1053
PciSI GCTCTTC 1 cut(s) 942
PdmI GAANNNNTTC 2 cut(s) 936, 989
PfeI GAWTC 6 cut(s) 28, 257, 618, 794, 800, 1144
PflMI CCANNNNNTGG 2 cut(s) 533, 557
PfoI TCCNGGA 1 cut(s) 586
PkrI GCNGC 5 cut(s) 124, 127, 296, 577, 816
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 2 cut(s) 505, 634
PpsI GAGTC 2 cut(s) 505, 634
PshAI GACNNNNGTC 1 cut(s) 1049
Psp6I CCWGG 1 cut(s) 586
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 3 cut(s) 148, 744, 1157
PspPI GGNCC 1 cut(s) 715
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 742
PvuI CGATCG 1 cut(s) 187
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 4 cut(s) 395, 882, 1079, 1179
SatI GCNGC 5 cut(s) 123, 126, 295, 576, 815
Sau96I GGNCC 1 cut(s) 715
SchI GAGTC 2 cut(s) 505, 634
ScrFI CCNGG 2 cut(s) 588, 625
SduI GDGCHC 3 cut(s) 149, 961, 1220
SfaNI GCATC 4 cut(s) 640, 895, 898, 1013
SfuI TTCGAA 2 cut(s) 103, 1206
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 1078
SmoI CTYRAG 1 cut(s) 1078
Sse9I AATT 5 cut(s) 465, 633, 765, 870, 1031
SsiI CCGC 6 cut(s) 123, 126, 321, 383, 575, 1069
StyD4I CCNGG 2 cut(s) 586, 623
StyI CCWWGG 3 cut(s) 117, 613, 1012
TaaI ACNGT 2 cut(s) 913, 1167
TaiI ACGT 1 cut(s) 558
TaqI TCGA 6 cut(s) 103, 183, 638, 896, 1037, 1206
TasI AATT 5 cut(s) 465, 633, 765, 870, 1031
TatI WGTACW 1 cut(s) 231
TauI GCSGC 3 cut(s) 125, 128, 578
TfiI GAWTC 6 cut(s) 28, 257, 618, 794, 800, 1144
Tru1I TTAA 4 cut(s) 395, 882, 1079, 1179
Tru9I TTAA 4 cut(s) 395, 882, 1079, 1179
TscAI CASTG 4 cut(s) 257, 385, 1047, 1128
TseFI GTSAC 1 cut(s) 1161
TseI GCWGC 2 cut(s) 294, 814
Tsp45I GTSAC 1 cut(s) 1161
TspDTI ATGAA 4 cut(s) 224, 427, 945, 1070
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 4 cut(s) 257, 385, 1047, 1128
Van91I CCANNNNNTGG 2 cut(s) 533, 557
Vha464I CTTAAG 1 cut(s) 1078
XapI RAATTY 1 cut(s) 633
XmnI GAANNNNTTC 2 cut(s) 936, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.