Rh2AG057900

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014310
Physical Location & Seq
Forward (+)
4688818 .. 4690662
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG057900.1

Sequence Viewer

Length: 861 bp
ATGTTAGAAACTAAGAGCCTTTCATCAGCCATGAACGCTAAAATCGTGGGCACGGGGAGTGAGTCCATAGTCTTAGCACATGGCTATGGGGGAGACCAGTCTGTGTGGGACAAAATCCTTCCCTACTTGGCTCAACATTACAGTGTTCTTGTTTTCGACTGGACCTTTTCGGGTGCTGCTAAGGACCCCAACCTCTTTGATCCTGCCAAATATTCGAGCTACGATGATTTTGCTCATGACTTGATTGCTCTATTGGATGAACTTAACTTGAAATCCTCAGTCTATATTGGCCACTCCATGTCTGGTATGGTTGGATGCATTGCCTCCATCAAGAGACCTGACCTCTTTAGCAGCCTCATACTTCTTGGAGCTTCTCCTAGGTATATGAATACAGATGACTATGAAGGTGGGTTTGGGAGTTCAGACATTAAACAAATCCTCTCAAGCATAGAATCCAACTTTCACAACTGGGCTGCTCACTTTGCTCCCCTTGTTGTGGACTCAAATGACCCTCTCTCTGTTGACAAGTTTACCAAGTGCCTGCAAAAAATGAGACCTGAATTTGCACTTGCTGTAGGAAAAATCGTCTTTTACAGCGACGAAAGAGACACTCTCGACAAGGTCTCAACTCCATGCACTATCATCCACACCAGCAGTGACATTGTCTCTCCAAAATCGGTCGCGTTTTACATGCAAAAGAAAATCAAGGAAAACCCCAAAGTGGAGATTATGAATACTAATGGCCATTTCCCACAGTTGACTGCACACATTCAGCTTCTTGATGTGCTAGGACTCATCTTGGGGGTTGAATTGGATCTTGATCTTGATCTTAAACCAAGATTGGGCAAATTATCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

286

Amino Acids

31.5

Weight (kDa)

5.42

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 22 - 236 1.1e-08 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 23 - 254 5.5e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 23 - 254 4.8e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24420
fragaria_vesca FvH4_1g05320
malus_domestica MD02G1056400.v1.1 MD15G1189400.v1.1
prunus_persica Prupe.7G227500_v2.0.a1
pyrus_communis pycom02g04550 pycom15g16980
rosa_chinensis RchiOBHm_Chr2g0091021
rosa_laevigata RLG00000016167
rosa_multiflora Rmu_sc0003305.1_g000006
rosa_roxburghii Rroxscaffold_2G00150680
rosa_rugosa Rorug02G0011500
rosa_samantha Rh2AG057900 Rh2BG056200 Rh2CG058500 Rh2DG057600
rosa_wichuraiana Rw2G005140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 683
AclWI GGATC 2 cut(s) 194, 822
AcoI YGGCCR 2 cut(s) 289, 742
AcsI RAATTY 1 cut(s) 560
AfiI CCNNNNNNNGG 3 cut(s) 496, 721, 842
AgsI TTSAA 2 cut(s) 271, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
AluBI AGCT 3 cut(s) 219, 371, 775
AluI AGCT 3 cut(s) 219, 371, 775
Alw26I GTCTC 6 cut(s) 87, 328, 547, 600, 628, 670
AlwI GGATC 2 cut(s) 194, 822
AoxI GGCC 2 cut(s) 289, 742
ApeKI GCWGC 3 cut(s) 176, 351, 473
ApoI RAATTY 1 cut(s) 560
AspA2I CCTAGG 1 cut(s) 377
AspS9I GGNCC 2 cut(s) 162, 184
AvaII GGWCC 2 cut(s) 162, 184
AvrII CCTAGG 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 53
BalI TGGCCA 2 cut(s) 291, 744
BbvI GCAGC 3 cut(s) 163, 363, 460
BccI CCATC 1 cut(s) 335
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BcoDI GTCTC 6 cut(s) 87, 328, 547, 600, 628, 670
BfaI CTAG 2 cut(s) 378, 788
BfmI CTRYAG 1 cut(s) 573
BisI GCNGC 3 cut(s) 177, 352, 474
BlnI CCTAGG 1 cut(s) 377
BlsI GCNGC 3 cut(s) 178, 353, 475
Bme18I GGWCC 2 cut(s) 162, 184
BmgT120I GGNCC 2 cut(s) 162, 184
BmiI GGNNCC 1 cut(s) 186
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 1 cut(s) 305
BmuI ACTGGG 1 cut(s) 478
BplI GAGNNNNNCTC 2 cut(s) 597, 629
Bpu10I CCTNAGC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 427
BsaBI GATNNNNATC 2 cut(s) 819, 825
BsaI GGTCTC 4 cut(s) 87, 328, 547, 628
BsaJI CCNNGG 1 cut(s) 377
Bsc4I CCNNNNNNNGG 3 cut(s) 496, 721, 842
Bse1I ACTGG 3 cut(s) 97, 164, 473
Bse3DI GCAATG 1 cut(s) 318
Bse8I GATNNNNATC 2 cut(s) 819, 825
BseDI CCNNGG 1 cut(s) 377
BseGI GGATG 3 cut(s) 262, 320, 642
BseJI GATNNNNATC 2 cut(s) 819, 825
BseLI CCNNNNNNNGG 3 cut(s) 496, 721, 842
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 3 cut(s) 97, 164, 473
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 3 cut(s) 163, 363, 460
BsgI GTGCAG 1 cut(s) 747
Bsh1236I CGCG 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 681
BshFI GGCC 2 cut(s) 291, 744
BsiEI CGRYCG 1 cut(s) 681
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 3 cut(s) 496, 721, 842
BsmAI GTCTC 6 cut(s) 87, 328, 547, 600, 628, 670
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 2 cut(s) 291, 744
Bso31I GGTCTC 4 cut(s) 87, 328, 547, 628
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 4 cut(s) 199, 814, 820, 826
BspANI GGCC 2 cut(s) 291, 744
BspCNI CTCAG 1 cut(s) 290
BspFNI CGCG 1 cut(s) 683
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 186
BspPI GGATC 2 cut(s) 194, 822
BspTNI GGTCTC 4 cut(s) 87, 328, 547, 628
BsrDI GCAATG 1 cut(s) 318
BsrI ACTGG 3 cut(s) 97, 164, 473
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 4 cut(s) 199, 814, 820, 826
BssT1I CCWWGG 1 cut(s) 377
Bst4CI ACNGT 2 cut(s) 143, 756
BstC8I GCNNGC 1 cut(s) 542
BstDEI CTNAG 4 cut(s) 12, 73, 180, 277
BstF5I GGATG 3 cut(s) 262, 320, 642
BstFNI CGCG 1 cut(s) 683
BstKTI GATC 4 cut(s) 202, 817, 823, 829
BstMAI GTCTC 6 cut(s) 87, 328, 547, 600, 628, 670
BstMBI GATC 4 cut(s) 199, 814, 820, 826
BstMCI CGRYCG 1 cut(s) 681
BstMWI GCNNNNNNNGC 2 cut(s) 35, 482
BstNSI RCATGY 1 cut(s) 694
BstSFI CTRYAG 1 cut(s) 573
BstSLI GKGCMC 1 cut(s) 53
BstUI CGCG 1 cut(s) 683
BstV1I GCAGC 3 cut(s) 163, 363, 460
BstX2I RGATCY 1 cut(s) 814
BstYI RGATCY 1 cut(s) 814
BsuRI GGCC 2 cut(s) 291, 744
BtsCI GGATG 3 cut(s) 262, 320, 642
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 2 cut(s) 148, 661
Cac8I GCNNGC 1 cut(s) 542
CciI TCATGA 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 162, 184
CviAII CATG 7 cut(s) 31, 80, 236, 298, 633, 691, 855
DdeI CTNAG 4 cut(s) 12, 73, 180, 277
DpnI GATC 4 cut(s) 201, 816, 822, 828
DpnII GATC 4 cut(s) 199, 814, 820, 826
EaeI YGGCCR 2 cut(s) 289, 742
Eco130I CCWWGG 1 cut(s) 377
Eco31I GGTCTC 4 cut(s) 87, 328, 547, 628
Eco47I GGWCC 2 cut(s) 162, 184
EcoO109I RGGNCCY 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 377
EcoT22I ATGCAT 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 7 cut(s) 34, 83, 239, 301, 636, 694, 858
FaqI GGGAC 1 cut(s) 122
FatI CATG 7 cut(s) 30, 79, 235, 297, 632, 690, 854
Fnu4HI GCNGC 3 cut(s) 177, 352, 474
FokI GGATG 3 cut(s) 269, 327, 629
Fsp4HI GCNGC 3 cut(s) 177, 352, 474
FspBI CTAG 2 cut(s) 378, 788
GluI GCNGC 3 cut(s) 177, 352, 474
HaeIII GGCC 2 cut(s) 291, 744
Hin1II CATG 7 cut(s) 34, 83, 239, 301, 636, 694, 858
HincII GTYRAC 2 cut(s) 523, 759
HindII GTYRAC 2 cut(s) 523, 759
HinfI GANTC 4 cut(s) 62, 452, 500, 792
Hpy166II GTNNAC 4 cut(s) 499, 523, 531, 759
Hpy188I TCNGA 1 cut(s) 424
Hpy188III TCNNGA 6 cut(s) 236, 331, 614, 779, 818, 824
Hpy8I GTNNAC 4 cut(s) 499, 523, 531, 759
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 2 cut(s) 128, 398
HpyCH4III ACNGT 2 cut(s) 143, 756
HpyCH4V TGCA 6 cut(s) 318, 544, 566, 636, 694, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 482
HpyF3I CTNAG 4 cut(s) 12, 73, 180, 277
Hsp92II CATG 7 cut(s) 34, 83, 239, 301, 636, 694, 858
Kzo9I GATC 4 cut(s) 199, 814, 820, 826
LmnI GCTCC 2 cut(s) 368, 490
LpnPI CCDG 9 cut(s) 110, 145, 216, 288, 351, 454, 554, 570, 664
Lsp1109I GCAGC 3 cut(s) 163, 363, 460
LweI GCATC 1 cut(s) 305
MaeI CTAG 2 cut(s) 378, 788
MaeIII GTNAC 1 cut(s) 656
MalI GATC 4 cut(s) 201, 816, 822, 828
MboI GATC 4 cut(s) 199, 814, 820, 826
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 1 cut(s) 53
MlsI TGGCCA 2 cut(s) 291, 744
MluCI AATT 3 cut(s) 560, 809, 848
MluNI TGGCCA 2 cut(s) 291, 744
MlyI GAGTC 3 cut(s) 71, 494, 786
MmeI TCCRAC 2 cut(s) 292, 480
MnlI CCTC 7 cut(s) 203, 286, 334, 353, 365, 449, 522
Mox20I TGGCCA 2 cut(s) 291, 744
Mph1103I ATGCAT 1 cut(s) 320
MscI TGGCCA 2 cut(s) 291, 744
MseI TTAA 3 cut(s) 264, 429, 831
MslI CAYNNNNRTG 2 cut(s) 84, 141
Msp20I TGGCCA 2 cut(s) 291, 744
MvnI CGCG 1 cut(s) 683
MwoI GCNNNNNNNGC 2 cut(s) 35, 482
NdeII GATC 4 cut(s) 199, 814, 820, 826
NlaIII CATG 7 cut(s) 34, 83, 239, 301, 636, 694, 858
NlaIV GGNNCC 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 656
NsiI ATGCAT 1 cut(s) 320
NspI RCATGY 1 cut(s) 694
PagI TCATGA 1 cut(s) 235
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 1 cut(s) 452
PflFI GACNNNGTC 2 cut(s) 620, 662
PkrI GCNGC 3 cut(s) 178, 353, 475
PleI GAGTC 3 cut(s) 70, 494, 786
PpsI GAGTC 3 cut(s) 70, 494, 786
PpuMI RGGWCCY 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 2 cut(s) 162, 184
PspPPI RGGWCCY 1 cut(s) 184
PsuI RGATCY 1 cut(s) 814
PsyI GACNNNGTC 2 cut(s) 620, 662
RseI CAYNNNNRTG 2 cut(s) 84, 141
SaqAI TTAA 3 cut(s) 264, 429, 831
SatI GCNGC 3 cut(s) 177, 352, 474
Sau3AI GATC 4 cut(s) 199, 814, 820, 826
Sau96I GGNCC 2 cut(s) 162, 184
SchI GAGTC 3 cut(s) 71, 494, 786
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 1 cut(s) 305
SfcI CTRYAG 1 cut(s) 573
SinI GGWCC 2 cut(s) 162, 184
SmiMI CAYNNNNRTG 2 cut(s) 84, 141
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
Sse9I AATT 3 cut(s) 560, 809, 848
SspI AATATT 1 cut(s) 212
SspMI CTAG 2 cut(s) 378, 788
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 2 cut(s) 143, 756
TaqI TCGA 3 cut(s) 156, 215, 615
TaqII GACCGA 1 cut(s) 667
TasI AATT 3 cut(s) 560, 809, 848
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 3 cut(s) 264, 429, 831
Tru9I TTAA 3 cut(s) 264, 429, 831
TscAI CASTG 2 cut(s) 148, 661
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 3 cut(s) 176, 351, 473
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 6 cut(s) 12, 47, 273, 401, 417, 746
TspRI CASTG 2 cut(s) 148, 661
Tth111I GACNNNGTC 2 cut(s) 620, 662
VpaK11BI GGWCC 2 cut(s) 162, 184
XapI RAATTY 1 cut(s) 560
XceI RCATGY 1 cut(s) 694
XcmI CCANNNNNNNNNTGG 2 cut(s) 299, 304
XmaJI CCTAGG 1 cut(s) 377
XspI CTAG 2 cut(s) 378, 788
Zsp2I ATGCAT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.