MD02G1056400.v1.1

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014310
Physical Location & Seq
Forward (+)
4568148 .. 4570879
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1056400.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGTGCTAGAAAAGACCCTTTCGACCGCCATGAACGCAAAAATCGTGGGGTCGGGCGGCGAAGCGGTGGTTTTAGCACATGGTTACGGAGGAGACCAGTCTGTGTGGGACAAAATCCTGCCTTACTTGTCTCAGCATTACCGTGTTCTCGTCTTCGACTGGCCCTTCTCCGGCGCTGTGAATAATAAGGATGGTGATAACCTGAAACTGTTTGATCCAGCTGGGAAATATTCCTCCTATGACGCATTTGCCCTTGACCTAATTGCTCTCTTAGACGAGTTTAACCTAAAATCTACTGTCTTTGTTGGTCATTCCATGTCTGGTATGATTGGATGCATTGCTTCAGTTAAAAGACCTGACCTCTTTAACTCCCTCATCCTCATTGGAGCTTCTCCTAGGTATATAAATACAGATGACTATGAAGGTGGATTTGAGATGTCAGATATTACACAAATCATCTCAGCCATAGAATCCAACTATCACAACTGGGCGGCCAACTTCGCCACACTTGCTGTGGACACAAATGACCCTCCCTCTGTCGAAAAATTCGCCAAGTGCCTGCAGAGAATGAGGCCTGAAGTTGCCCTCGCCTTGGCCAAAACCGTCTTTTACAGCGACCATAGAGACGTTATCGACAAGGTCTCGACTCCATGCACCATCGTCAGCACCAGCAGCGACATGGCTGCTCCAAAAACGGTTGCGTTCTACATGCAGAAGAAGATGAAGGAAAACTCTAATGTCGAGATTATGAATACAAATGGGCATTTCCCACAGTTGACTGCCCACCTTGAGCTTCTTGATGTGCTTGGAAAAGTCTTGGGCCTTAAATTTGATCATGATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

282

Amino Acids

30.91

Weight (kDa)

5.32

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 22 - 260 2.2e-16 alpha/beta hydrolase fold
Hydrolase_4 PF12146 22 - 238 3.6e-06 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 23 - 264 1.4e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24420
fragaria_vesca FvH4_1g05320
malus_domestica MD02G1056400.v1.1 MD15G1189400.v1.1
prunus_persica Prupe.7G227500_v2.0.a1
pyrus_communis pycom02g04550 pycom15g16980
rosa_chinensis RchiOBHm_Chr2g0091021
rosa_laevigata RLG00000016167
rosa_multiflora Rmu_sc0003305.1_g000006
rosa_roxburghii Rroxscaffold_2G00150680
rosa_rugosa Rorug02G0011500
rosa_samantha Rh2AG057900 Rh2BG056200 Rh2CG058500 Rh2DG057600
rosa_wichuraiana Rw2G005140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 27, 57, 65, 491
AclWI GGATC 2 cut(s) 209, 833
AcoI YGGCCR 2 cut(s) 492, 594
AcsI RAATTY 2 cut(s) 545, 827
AcuI CTGAAG 2 cut(s) 327, 597
AfiI CCNNNNNNNGG 2 cut(s) 170, 592
AluBI AGCT 3 cut(s) 221, 389, 793
AluI AGCT 3 cut(s) 221, 389, 793
Alw26I GTCTC 4 cut(s) 87, 135, 618, 646
AlwI GGATC 2 cut(s) 209, 833
AoxI GGCC 5 cut(s) 161, 492, 572, 594, 820
ApeKI GCWGC 2 cut(s) 672, 683
ApoI RAATTY 2 cut(s) 545, 827
AspA2I CCTAGG 1 cut(s) 395
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 162, 820
AsuHPI GGTGA 1 cut(s) 206
AvrII CCTAGG 1 cut(s) 395
BalI TGGCCA 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 145
BbvI GCAGC 2 cut(s) 670, 684
BccI CCATC 2 cut(s) 185, 665
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BclI TGATCA 1 cut(s) 832
BcoDI GTCTC 4 cut(s) 87, 135, 618, 646
BfaI CTAG 2 cut(s) 8, 396
BfmI CTRYAG 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 4 cut(s) 58, 492, 673, 684
BlnI CCTAGG 1 cut(s) 395
BlsI GCNGC 4 cut(s) 59, 493, 674, 685
BmgT120I GGNCC 2 cut(s) 162, 820
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 323
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 837
BsaI GGTCTC 2 cut(s) 87, 646
BsaJI CCNNGG 2 cut(s) 395, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 592
Bse1I ACTGG 3 cut(s) 97, 164, 491
Bse3DI GCAATG 1 cut(s) 336
Bse8I GATNNNNATC 1 cut(s) 837
BseDI CCNNGG 2 cut(s) 395, 591
BseGI GGATG 3 cut(s) 196, 338, 375
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 2 cut(s) 170, 592
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 146, 474
BseNI ACTGG 3 cut(s) 97, 164, 491
BseRI GAGGAG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 670, 684
BseYI CCCAGC 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 5 cut(s) 163, 494, 574, 596, 822
BsiEI CGRYCG 1 cut(s) 27
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 170, 592
BsmAI GTCTC 4 cut(s) 87, 135, 618, 646
BsmBI CGTCTC 1 cut(s) 618
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 5 cut(s) 163, 494, 574, 596, 822
Bso31I GGTCTC 2 cut(s) 87, 646
Bsp143I GATC 3 cut(s) 214, 832, 838
BspACI CCGC 4 cut(s) 27, 57, 65, 491
BspANI GGCC 5 cut(s) 163, 494, 574, 596, 822
BspCNI CTCAG 2 cut(s) 145, 473
BspHI TCATGA 1 cut(s) 835
BspMAI CTGCAG 1 cut(s) 564
BspPI GGATC 2 cut(s) 209, 833
BspTNI GGTCTC 2 cut(s) 87, 646
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 3 cut(s) 97, 164, 491
BssECI CCNNGG 2 cut(s) 395, 591
BssMI GATC 3 cut(s) 214, 832, 838
BssT1I CCWWGG 2 cut(s) 395, 591
Bst4CI ACNGT 6 cut(s) 143, 210, 298, 604, 697, 774
BstC8I GCNNGC 1 cut(s) 560
BstDEI CTNAG 3 cut(s) 132, 271, 460
BstF5I GGATG 3 cut(s) 196, 338, 375
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 3 cut(s) 217, 835, 841
BstMAI GTCTC 4 cut(s) 87, 135, 618, 646
BstMBI GATC 3 cut(s) 214, 832, 838
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 4 cut(s) 35, 500, 509, 672
BstNSI RCATGY 1 cut(s) 712
BstSFI CTRYAG 1 cut(s) 560
BstV1I GCAGC 2 cut(s) 670, 684
BstV2I GAAGAC 1 cut(s) 145
BsuRI GGCC 5 cut(s) 163, 494, 574, 596, 822
BtsCI GGATG 3 cut(s) 196, 338, 375
Cac8I GCNNGC 1 cut(s) 560
CciI TCATGA 1 cut(s) 835
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 162, 820
CseI GACGC 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 27, 62
CviAII CATG 7 cut(s) 31, 80, 316, 651, 679, 709, 836
DdeI CTNAG 3 cut(s) 132, 271, 460
DpnI GATC 3 cut(s) 216, 834, 840
DpnII GATC 3 cut(s) 214, 832, 838
EaeI YGGCCR 2 cut(s) 492, 594
Eco130I CCWWGG 2 cut(s) 395, 591
Eco147I AGGCCT 1 cut(s) 574
Eco31I GGTCTC 2 cut(s) 87, 646
Eco57I CTGAAG 2 cut(s) 327, 597
EcoT14I CCWWGG 2 cut(s) 395, 591
EcoT22I ATGCAT 1 cut(s) 338
ErhI CCWWGG 2 cut(s) 395, 591
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 7 cut(s) 34, 83, 319, 654, 682, 712, 839
FaqI GGGAC 1 cut(s) 122
FatI CATG 7 cut(s) 30, 79, 315, 650, 678, 708, 835
FbaI TGATCA 1 cut(s) 832
Fnu4HI GCNGC 4 cut(s) 58, 492, 673, 684
FokI GGATG 3 cut(s) 203, 345, 362
Fsp4HI GCNGC 4 cut(s) 58, 492, 673, 684
FspBI CTAG 2 cut(s) 8, 396
GlaI GCGC 1 cut(s) 175
GluI GCNGC 4 cut(s) 58, 492, 673, 684
GsaI CCCAGC 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 5 cut(s) 163, 494, 574, 596, 822
HapII CCGG 1 cut(s) 171
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 176
Hin1II CATG 7 cut(s) 34, 83, 319, 654, 682, 712, 839
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HinfI GANTC 2 cut(s) 470, 646
HpaII CCGG 1 cut(s) 171
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 2 cut(s) 517, 777
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 4 cut(s) 643, 742, 797, 836
Hpy8I GTNNAC 2 cut(s) 517, 777
HpyAV CCTTC 3 cut(s) 175, 416, 718
HpyCH4III ACNGT 6 cut(s) 143, 210, 298, 604, 697, 774
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 4 cut(s) 336, 562, 654, 712
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 500, 509, 672
HpyF3I CTNAG 3 cut(s) 132, 271, 460
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 7 cut(s) 34, 83, 319, 654, 682, 712, 839
HspAI GCGC 1 cut(s) 174
Ksp22I TGATCA 1 cut(s) 832
Kzo9I GATC 3 cut(s) 214, 832, 838
LmnI GCTCC 2 cut(s) 386, 691
Lsp1109I GCAGC 2 cut(s) 670, 684
LweI GCATC 1 cut(s) 323
MaeI CTAG 2 cut(s) 8, 396
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 1 cut(s) 83
MalI GATC 3 cut(s) 216, 834, 840
MboI GATC 3 cut(s) 214, 832, 838
MboII GAAGA 3 cut(s) 145, 727, 730
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 3 cut(s) 261, 545, 827
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 9 cut(s) 83, 244, 371, 383, 389, 540, 544, 564, 596
Mox20I TGGCCA 1 cut(s) 596
Mph1103I ATGCAT 1 cut(s) 338
MscI TGGCCA 1 cut(s) 596
MseI TTAA 5 cut(s) 282, 348, 366, 825, 844
MslI CAYNNNNRTG 1 cut(s) 141
Msp20I TGGCCA 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 171
MwoI GCNNNNNNNGC 4 cut(s) 35, 500, 509, 672
NdeII GATC 3 cut(s) 214, 832, 838
NlaIII CATG 7 cut(s) 34, 83, 319, 654, 682, 712, 839
NsiI ATGCAT 1 cut(s) 338
NspI RCATGY 1 cut(s) 712
PagI TCATGA 1 cut(s) 835
PceI AGGCCT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 1 cut(s) 470
PflFI GACNNNGTC 1 cut(s) 638
PkrI GCNGC 4 cut(s) 59, 493, 674, 685
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
PspFI CCCAGC 1 cut(s) 221
PspPI GGNCC 2 cut(s) 162, 820
PstI CTGCAG 1 cut(s) 564
PsyI GACNNNGTC 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 221
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 5 cut(s) 282, 348, 366, 825, 844
SatI GCNGC 4 cut(s) 58, 492, 673, 684
Sau3AI GATC 3 cut(s) 214, 832, 838
Sau96I GGNCC 2 cut(s) 162, 820
SchI GAGTC 1 cut(s) 640
SfaNI GCATC 1 cut(s) 323
SfcI CTRYAG 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 3 cut(s) 261, 545, 827
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 4 cut(s) 27, 57, 65, 491
SspI AATATT 1 cut(s) 230
SspMI CTAG 2 cut(s) 8, 396
StuI AGGCCT 1 cut(s) 574
StyI CCWWGG 2 cut(s) 395, 591
TaaI ACNGT 6 cut(s) 143, 210, 298, 604, 697, 774
TaiI ACGT 1 cut(s) 630
TaqI TCGA 6 cut(s) 23, 156, 540, 633, 644, 741
TasI AATT 3 cut(s) 261, 545, 827
TauI GCSGC 2 cut(s) 60, 494
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 5 cut(s) 282, 348, 366, 825, 844
Tru9I TTAA 5 cut(s) 282, 348, 366, 825, 844
TseI GCWGC 2 cut(s) 672, 683
TspDTI ATGAA 4 cut(s) 47, 435, 737, 764
TspGWI ACGGA 1 cut(s) 102
Tth111I GACNNNGTC 1 cut(s) 638
XapI RAATTY 2 cut(s) 545, 827
XceI RCATGY 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 2 cut(s) 511, 676
XmaJI CCTAGG 1 cut(s) 395
XspI CTAG 2 cut(s) 8, 396
Zsp2I ATGCAT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.