pycom15g16980

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014310
Physical Location & Seq
Forward (+)
11923942 .. 11925394
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g16980.2

Sequence Viewer

Length: 858 bp
ATGGTGCTAGAAAAAAACCTTTCGACCGCCATGAACGCAAGAATCGTGGGCTCGGGCGGTGAAGCGGTGGTTCTAGCACATGGTTACGGAGGAGACCAGTCTGTGTGGGACAAAATCGTGCCCTACTTGGCTCAGCTTTACCGTGTTCTTGTTTTCGACTGGCCTTTCTCCGGCGCTGTGAATAATAAGGAAGGTGATTACCTGAAACTGTTTGATCCAGCTGGGAAATATTCCTCCTATGACGCATTTGCCCTTGACCTAATTGCTCTCTTAGACGAGTTCAACCTAAAATCTACTGTCTTTGTTGGTCATTCCATGTCTGGTATGATTGGATGTATTGCTTCAGTTAAAAGACCTGACCTCTTTAAGTCCCTCATCCTCATTGGAGCTTCTCCTAGGTATATAAATATAGACGACTATGAAGGTGGGTTTGAGATGTCAGATATCACACAGATCATCTCAGCCATAGAAACCAACTATCAAAACTGGGCTGCCAACTTCGCCACACTTGCTGTGGACACAAATGATCCTCTCTCGGTCGAAAAATTCGTCAAGTGCCTGCGCAGAATGAGGCCTGAAATTGCCCTCGCCTTGGCGAAAACCGTCTTTTACAGCGACCATAGAGACATTATAGACAAGGTCTCGACTCCATGCACCATCATCAGCACCAGCAGCGACGTTGTTGCTCCAAAATCAGTTGCGTTCTACATGCAGAAGAAGATGAAGGAAAACCCTAATGTCGAGATTATGAATACAGACGGGCATTTCCCGCAGTTGACTGCACACCTTGAGCTTCTTGATGTGCTAGGACAAGTCTTGGGGCTTAAATTTGATCAAGATCCTTATATCATTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

286

Amino Acids

31.54

Weight (kDa)

5.04

Isoelectric Point (pI)

29.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24420
fragaria_vesca FvH4_1g05320
malus_domestica MD02G1056400.v1.1 MD15G1189400.v1.1
prunus_persica Prupe.7G227500_v2.0.a1
pyrus_communis pycom02g04550 pycom15g16980
rosa_chinensis RchiOBHm_Chr2g0091021
rosa_laevigata RLG00000016167
rosa_multiflora Rmu_sc0003305.1_g000006
rosa_roxburghii Rroxscaffold_2G00150680
rosa_rugosa Rorug02G0011500
rosa_samantha Rh2AG057900 Rh2BG056200 Rh2CG058500 Rh2DG057600
rosa_wichuraiana Rw2G005140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 563
AciI CCGC 4 cut(s) 27, 57, 65, 770
AclWI GGATC 3 cut(s) 209, 521, 833
AcsI RAATTY 2 cut(s) 545, 827
AcuI CTGAAG 1 cut(s) 327
AfiI CCNNNNNNNGG 2 cut(s) 170, 592
AgsI TTSAA 1 cut(s) 283
AluBI AGCT 4 cut(s) 136, 221, 389, 793
AluI AGCT 4 cut(s) 136, 221, 389, 793
Alw26I GTCTC 3 cut(s) 87, 618, 646
AlwI GGATC 3 cut(s) 209, 521, 833
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 2 cut(s) 161, 572
ApeKI GCWGC 2 cut(s) 491, 672
ApoI RAATTY 2 cut(s) 545, 827
ArsI GACNNNNNNTTYG 2 cut(s) 534, 566
AspA2I CCTAGG 1 cut(s) 395
AspLEI GCGC 2 cut(s) 176, 564
AsuHPI GGTGA 2 cut(s) 71, 206
AvaI CYCGRG 1 cut(s) 52
AvrII CCTAGG 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 123
BanII GRGCYC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 478, 684
BccI CCATC 1 cut(s) 665
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BclI TGATCA 1 cut(s) 832
BcoDI GTCTC 3 cut(s) 87, 618, 646
BfaI CTAG 4 cut(s) 8, 74, 396, 806
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 2 cut(s) 492, 673
BlnI CCTAGG 1 cut(s) 395
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 2 cut(s) 493, 674
BmeT110I CYCGRG 1 cut(s) 52
BmrI ACTGGG 1 cut(s) 496
BmuI ACTGGG 1 cut(s) 496
Bpu1102I GCTNAGC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 837
BsaI GGTCTC 2 cut(s) 87, 646
BsaJI CCNNGG 2 cut(s) 395, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 592
Bse1I ACTGG 3 cut(s) 97, 164, 491
Bse8I GATNNNNATC 1 cut(s) 837
BseDI CCNNGG 2 cut(s) 395, 591
BseGI GGATG 2 cut(s) 338, 375
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 2 cut(s) 170, 592
BseMII CTCAG 2 cut(s) 146, 474
BseNI ACTGG 3 cut(s) 97, 164, 491
BseRI GAGGAG 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 123
BseXI GCAGC 2 cut(s) 478, 684
BseYI CCCAGC 1 cut(s) 221
BsgI GTGCAG 1 cut(s) 765
Bsh1285I CGRYCG 2 cut(s) 27, 540
BshFI GGCC 2 cut(s) 163, 574
BsiEI CGRYCG 2 cut(s) 27, 540
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 2 cut(s) 122, 355
BslI CCNNNNNNNGG 2 cut(s) 170, 592
BsmAI GTCTC 3 cut(s) 87, 618, 646
BsmFI GGGAC 2 cut(s) 122, 355
BsnI GGCC 2 cut(s) 163, 574
Bso31I GGTCTC 2 cut(s) 87, 646
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 53, 123
Bsp143I GATC 5 cut(s) 214, 453, 526, 832, 838
Bsp1720I GCTNAGC 1 cut(s) 132
BspACI CCGC 4 cut(s) 27, 57, 65, 770
BspANI GGCC 2 cut(s) 163, 574
BspCNI CTCAG 2 cut(s) 145, 473
BspPI GGATC 3 cut(s) 209, 521, 833
BspTNI GGTCTC 2 cut(s) 87, 646
BsrI ACTGG 3 cut(s) 97, 164, 491
BssECI CCNNGG 2 cut(s) 395, 591
BssMI GATC 5 cut(s) 214, 453, 526, 832, 838
BssT1I CCWWGG 2 cut(s) 395, 591
Bst4CI ACNGT 4 cut(s) 143, 210, 298, 604
BstC8I GCNNGC 1 cut(s) 560
BstDEI CTNAG 3 cut(s) 132, 271, 460
BstF5I GGATG 2 cut(s) 338, 375
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 2 cut(s) 176, 564
BstKTI GATC 5 cut(s) 217, 456, 529, 835, 841
BstMAI GTCTC 3 cut(s) 87, 618, 646
BstMBI GATC 5 cut(s) 214, 453, 526, 832, 838
BstMCI CGRYCG 2 cut(s) 27, 540
BstMWI GCNNNNNNNGC 5 cut(s) 35, 500, 509, 672, 769
BstNSI RCATGY 1 cut(s) 712
BstSLI GKGCMC 1 cut(s) 123
BstV1I GCAGC 2 cut(s) 478, 684
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BsuRI GGCC 2 cut(s) 163, 574
BtsCI GGATG 2 cut(s) 338, 375
Cac8I GCNNGC 1 cut(s) 560
CfoI GCGC 2 cut(s) 176, 564
CseI GACGC 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 27, 62
CviAII CATG 5 cut(s) 31, 80, 316, 651, 709
DdeI CTNAG 3 cut(s) 132, 271, 460
DpnI GATC 5 cut(s) 216, 455, 528, 834, 840
DpnII GATC 5 cut(s) 214, 453, 526, 832, 838
Eco130I CCWWGG 2 cut(s) 395, 591
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 2 cut(s) 87, 646
Eco32I GATATC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 52
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 2 cut(s) 395, 591
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 395, 591
FaeI CATG 5 cut(s) 34, 83, 319, 654, 712
FaqI GGGAC 2 cut(s) 122, 355
FatI CATG 5 cut(s) 30, 79, 315, 650, 708
FauI CCCGC 1 cut(s) 777
FbaI TGATCA 1 cut(s) 832
Fnu4HI GCNGC 2 cut(s) 492, 673
FokI GGATG 2 cut(s) 345, 362
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 492, 673
FspBI CTAG 4 cut(s) 8, 74, 396, 806
FspI TGCGCA 1 cut(s) 563
GlaI GCGC 2 cut(s) 175, 563
GluI GCNGC 2 cut(s) 492, 673
GsaI CCCAGC 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 2 cut(s) 163, 574
HapII CCGG 1 cut(s) 171
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 176, 564
Hin1II CATG 5 cut(s) 34, 83, 319, 654, 712
Hin6I GCGC 2 cut(s) 174, 562
HinP1I GCGC 2 cut(s) 174, 562
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HinfI GANTC 2 cut(s) 42, 646
HpaII CCGG 1 cut(s) 171
HphI GGTGA 2 cut(s) 71, 206
Hpy166II GTNNAC 2 cut(s) 517, 777
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 4 cut(s) 643, 742, 797, 836
Hpy8I GTNNAC 2 cut(s) 517, 777
Hpy99I CGWCG 1 cut(s) 680
HpyAV CCTTC 3 cut(s) 185, 416, 718
HpyCH4III ACNGT 4 cut(s) 143, 210, 298, 604
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 3 cut(s) 654, 712, 782
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 500, 509, 672, 769
HpyF3I CTNAG 3 cut(s) 132, 271, 460
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 5 cut(s) 34, 83, 319, 654, 712
HspAI GCGC 2 cut(s) 174, 562
Ksp22I TGATCA 1 cut(s) 832
Kzo9I GATC 5 cut(s) 214, 453, 526, 832, 838
LmnI GCTCC 2 cut(s) 386, 691
Lsp1109I GCAGC 2 cut(s) 478, 684
MaeI CTAG 4 cut(s) 8, 74, 396, 806
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 1 cut(s) 83
MalI GATC 5 cut(s) 216, 455, 528, 834, 840
MboI GATC 5 cut(s) 214, 453, 526, 832, 838
MboII GAAGA 2 cut(s) 727, 730
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 2 cut(s) 53, 123
MluCI AATT 4 cut(s) 261, 545, 579, 827
MlyI GAGTC 1 cut(s) 640
MnlI CCTC 8 cut(s) 83, 244, 371, 383, 389, 540, 564, 596
MseI TTAA 3 cut(s) 348, 366, 825
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 171
MwoI GCNNNNNNNGC 5 cut(s) 35, 500, 509, 672, 769
NdeII GATC 5 cut(s) 214, 453, 526, 832, 838
NlaIII CATG 5 cut(s) 34, 83, 319, 654, 712
NsbI TGCGCA 1 cut(s) 563
NspI RCATGY 1 cut(s) 712
PceI AGGCCT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 546
PfeI GAWTC 1 cut(s) 42
PflFI GACNNNGTC 1 cut(s) 638
PkrI GCNGC 2 cut(s) 493, 674
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
PspFI CCCAGC 1 cut(s) 221
PsuI RGATCY 1 cut(s) 838
PsyI GACNNNGTC 1 cut(s) 638
PvuII CAGCTG 1 cut(s) 221
SaqAI TTAA 3 cut(s) 348, 366, 825
SatI GCNGC 2 cut(s) 492, 673
Sau3AI GATC 5 cut(s) 214, 453, 526, 832, 838
SchI GAGTC 1 cut(s) 640
SduI GDGCHC 2 cut(s) 53, 123
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 4 cut(s) 261, 545, 579, 827
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 4 cut(s) 27, 57, 65, 770
SspI AATATT 1 cut(s) 230
SspMI CTAG 4 cut(s) 8, 74, 396, 806
StuI AGGCCT 1 cut(s) 574
StyI CCWWGG 2 cut(s) 395, 591
TaaI ACNGT 4 cut(s) 143, 210, 298, 604
TaiI ACGT 1 cut(s) 681
TaqI TCGA 5 cut(s) 23, 156, 540, 644, 741
TaqII GACCGA 1 cut(s) 526
TasI AATT 4 cut(s) 261, 545, 579, 827
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 3 cut(s) 348, 366, 825
Tru9I TTAA 3 cut(s) 348, 366, 825
TseI GCWGC 2 cut(s) 491, 672
TspDTI ATGAA 4 cut(s) 47, 435, 737, 764
TspGWI ACGGA 1 cut(s) 102
Tth111I GACNNNGTC 1 cut(s) 638
XapI RAATTY 2 cut(s) 545, 827
XceI RCATGY 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XmaJI CCTAGG 1 cut(s) 395
XspI CTAG 4 cut(s) 8, 74, 396, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.