AT3G25900

homocysteine S-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012188
Physical Location & Seq
Forward (+)
9480746 .. 9482962
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25900.1

Sequence Viewer

Length: 981 bp
ATGGTTTTGGAGAAAAAATCTGCATTGTTGGAAGATTTGATAAAGAAATGCGGCGGTTGTGCGGTGGTCGACGGTGGTTTCGCTACCCAGCTTGAGATTCACGGCGCCGCCATTAACGACCCTTTGTGGAGTGCTGTTTCTCTCATCAAAAATCCTGAGCTCATCAAAAGGGTTCACATGGAATATTTGGAAGCTGGTGCAGACATTGTGGTTACTTCATCTTATCAGGCTACGATCCCGGGGTTTCTTTCTAGAGGTTTATCAATAGAAGAGAGCGAGTCTTTGCTTCAAAAGAGTGTAGAATTAGCTGTTGAAGCTCGTGATAGATTCTGGGAGAAAGTGAGCAAGGTTTCAGGACATAGCTATAACAGAGCTCTTGTAGCTGCATCTATTGGAAGCTATGGTGCTTATCTTGCTGATGGCTCTGAGTACAGTGGTCACTATGGTGAGAATGTAAGTTTGGATAAACTAAAGGACTTTCACAGGAGAAGACTTCAAGTCTTAGTGGAAGCTGGTCCTGATCTTCTTGCTTTTGAAACCATACCGAATAAACTCGAAGCTCAGGCTTGCGTTGAGCTTTTGGAGGAAGAGAAAGTGCAAATCCCAGCATGGATCTGCTTCACATCTGTAGATGGTGAGAAAGCTCCATCAGGAGAGAGCTTTGAGGAATGTCTTGAACCTCTTAACAAAAGCAACAATATTTACGCAGTTGGTATTAACTGCGCACCTCCTCAATTCATCGAAAATCTGATTCGCAAATTCGCCAAGCTGACGAAGAAAGCAATCGTTGTTTACCCGAATAGCGGAGAGGTTTGGGATGGAAAAGCTAAACAATGGCTGCCATCACAATGCTTTGGTGATGATGAATTTGAGATGTTTGCAACGAAATGGCGTGACCTTGGAGCTAAACTCATCGGAGGATGTTGCAGGACTACACCTTCCACCATTAACGCTATCTCCAGAGATTTGAAACGAAGATGA

Protein Analysis

326

Amino Acids

35.98

Weight (kDa)

5.47

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 22 - 321 6.1e-81 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 724
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 69
AciI CCGC 5 cut(s) 51, 54, 62, 108, 804
AclWI GGATC 2 cut(s) 229, 620
AcsI RAATTY 2 cut(s) 758, 866
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 803
AgsI TTSAA 6 cut(s) 290, 314, 497, 536, 677, 970
AhdI GACNNNNNGTC 1 cut(s) 497
AjuI GAANNNNNNNTTGG 2 cut(s) 443, 475
AleI CACNNNNGTG 1 cut(s) 444
Alw21I GWGCWC 2 cut(s) 162, 376
AlwI GGATC 2 cut(s) 229, 620
Ama87I CYCGRG 1 cut(s) 238
ApeKI GCWGC 2 cut(s) 383, 838
ApoI RAATTY 2 cut(s) 758, 866
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
AspLEI GCGC 2 cut(s) 107, 725
AspS9I GGNCC 1 cut(s) 515
AsuC2I CCSGG 2 cut(s) 239, 240
AsuHPI GGTGA 3 cut(s) 458, 647, 869
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 1 cut(s) 515
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 162, 376
BauI CACGAG 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 496
Bbv12I GWGCWC 2 cut(s) 162, 376
BbvI GCAGC 2 cut(s) 370, 825
BccI CCATC 5 cut(s) 413, 626, 655, 812, 850
BceAI ACGGC 1 cut(s) 118
BcnI CCSGG 2 cut(s) 239, 240
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 4 cut(s) 52, 108, 384, 839
BlsI GCNGC 4 cut(s) 53, 109, 385, 840
Bme1390I CCNGG 2 cut(s) 239, 240
Bme18I GGWCC 1 cut(s) 515
BmeRI GACNNNNNGTC 1 cut(s) 497
BmeT110I CYCGRG 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 515
BmiI GGNNCC 1 cut(s) 106
BmrFI CCNGG 2 cut(s) 239, 240
BmsI GCATC 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 496
BplI GAGNNNNNCTC 2 cut(s) 894, 926
BpmI CTGGAG 1 cut(s) 943
Bpu10I CCTNAGC 2 cut(s) 156, 561
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 2 cut(s) 239, 240
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 238, 239, 898
BsaXI ACNNNNNCTCC 2 cut(s) 941, 971
Bsc4I CCNNNNNNNGG 1 cut(s) 803
BseDI CCNNGG 3 cut(s) 238, 239, 898
BseGI GGATG 2 cut(s) 823, 926
BseLI CCNNNNNNNGG 1 cut(s) 803
BseMII CTCAG 3 cut(s) 147, 417, 575
BseRI GAGGAG 1 cut(s) 720
BseXI GCAGC 2 cut(s) 370, 825
BseYI CCCAGC 2 cut(s) 87, 604
BsgI GTGCAG 1 cut(s) 219
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 162, 376
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 803
BsoBI CYCGRG 1 cut(s) 238
Bsp1286I GDGCHC 2 cut(s) 162, 376
Bsp143I GATC 3 cut(s) 234, 520, 612
BspACI CCGC 5 cut(s) 51, 54, 62, 108, 804
BspCNI CTCAG 3 cut(s) 148, 418, 574
BspLI GGNNCC 1 cut(s) 106
BspPI GGATC 2 cut(s) 229, 620
BspT107I GGYRCC 1 cut(s) 104
BssECI CCNNGG 3 cut(s) 238, 239, 898
BssMI GATC 3 cut(s) 234, 520, 612
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 898
Bst2BI CACGAG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 74, 434
Bst6I CTCTTC 2 cut(s) 264, 582
BstACI GRCGYC 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 568
BstDEI CTNAG 4 cut(s) 156, 426, 502, 561
BstF5I GGATG 2 cut(s) 823, 926
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 2 cut(s) 107, 725
BstKTI GATC 3 cut(s) 237, 523, 615
BstMBI GATC 3 cut(s) 234, 520, 612
BstMWI GCNNNNNNNGC 3 cut(s) 314, 380, 413
BstSCI CCNGG 2 cut(s) 237, 238
BstSFI CTRYAG 1 cut(s) 627
BstV1I GCAGC 2 cut(s) 370, 825
BstV2I GAAGAC 1 cut(s) 496
BstX2I RGATCY 1 cut(s) 612
BstYI RGATCY 1 cut(s) 612
BtsCI GGATG 2 cut(s) 823, 926
BtsIMutI CAGTG 1 cut(s) 439
Cac8I GCNNGC 1 cut(s) 568
CfoI GCGC 2 cut(s) 107, 725
Cfr13I GGNCC 1 cut(s) 515
Cfr9I CCCGGG 1 cut(s) 238
Csp6I GTAC 1 cut(s) 430
CviAII CATG 2 cut(s) 178, 609
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 4 cut(s) 156, 426, 502, 561
DinI GGCGCC 1 cut(s) 106
DpnI GATC 3 cut(s) 236, 522, 614
DpnII GATC 3 cut(s) 234, 520, 612
DriI GACNNNNNGTC 1 cut(s) 497
Eam1104I CTCTTC 2 cut(s) 264, 582
Eam1105I GACNNNNNGTC 1 cut(s) 497
EarI CTCTTC 2 cut(s) 264, 582
Ecl136II GAGCTC 2 cut(s) 160, 374
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 2 cut(s) 162, 376
Eco47I GGWCC 1 cut(s) 515
Eco53kI GAGCTC 2 cut(s) 160, 374
Eco88I CYCGRG 1 cut(s) 238
EcoICRI GAGCTC 2 cut(s) 160, 374
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 2 cut(s) 162, 376
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 2 cut(s) 181, 612
FaiI YATR 7 cut(s) 179, 360, 366, 402, 444, 542, 610
FatI CATG 2 cut(s) 177, 608
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 4 cut(s) 52, 108, 384, 839
FokI GGATG 2 cut(s) 830, 933
FriOI GRGCYC 2 cut(s) 162, 376
Fsp4HI GCNGC 4 cut(s) 52, 108, 384, 839
FspBI CTAG 1 cut(s) 252
FspI TGCGCA 1 cut(s) 724
GlaI GCGC 2 cut(s) 106, 724
GluI GCNGC 4 cut(s) 52, 108, 384, 839
GsaI CCCAGC 2 cut(s) 91, 608
GsuI CTGGAG 1 cut(s) 943
HaeII RGCGCY 1 cut(s) 108
HapII CCGG 1 cut(s) 239
HhaI GCGC 2 cut(s) 107, 725
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 181, 612
Hin6I GCGC 2 cut(s) 105, 723
HinP1I GCGC 2 cut(s) 105, 723
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 4 cut(s) 97, 278, 327, 751
HpaII CCGG 1 cut(s) 239
HphI GGTGA 3 cut(s) 458, 647, 869
Hpy166II GTNNAC 3 cut(s) 70, 175, 793
Hpy188I TCNGA 3 cut(s) 427, 750, 917
Hpy188III TCNNGA 8 cut(s) 155, 252, 320, 354, 518, 651, 674, 960
Hpy8I GTNNAC 3 cut(s) 70, 175, 793
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 948
HpyCH4III ACNGT 2 cut(s) 74, 434
HpyCH4V TGCA 6 cut(s) 23, 200, 386, 598, 881, 927
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 380, 413
HpyF3I CTNAG 4 cut(s) 156, 426, 502, 561
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 181, 612
HspAI GCGC 2 cut(s) 105, 723
KasI GGCGCC 1 cut(s) 104
Kzo9I GATC 3 cut(s) 234, 520, 612
LmnI GCTCC 2 cut(s) 649, 902
Lsp1109I GCAGC 2 cut(s) 370, 825
LweI GCATC 1 cut(s) 395
MaeI CTAG 1 cut(s) 252
MaeIII GTNAC 3 cut(s) 211, 437, 893
MalI GATC 3 cut(s) 236, 522, 614
MboI GATC 3 cut(s) 234, 520, 612
MboII GAAGA 6 cut(s) 44, 281, 501, 515, 599, 787
MflI RGATCY 1 cut(s) 612
MhlI GDGCHC 2 cut(s) 162, 376
MluCI AATT 4 cut(s) 302, 734, 758, 866
Mly113I GGCGCC 1 cut(s) 105
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 8 cut(s) 248, 577, 658, 690, 738, 741, 802, 911
MseI TTAA 4 cut(s) 114, 684, 717, 948
MslI CAYNNNNRTG 2 cut(s) 444, 847
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 2 cut(s) 239, 240
MwoI GCNNNNNNNGC 3 cut(s) 314, 380, 413
NarI GGCGCC 1 cut(s) 105
NciI CCSGG 2 cut(s) 239, 240
NdeII GATC 3 cut(s) 234, 520, 612
NlaIII CATG 2 cut(s) 181, 612
NlaIV GGNNCC 1 cut(s) 106
NmuCI GTSAC 2 cut(s) 437, 893
NsbI TGCGCA 1 cut(s) 724
OliI CACNNNNGTG 1 cut(s) 444
PfeI GAWTC 3 cut(s) 97, 327, 751
PkrI GCNGC 4 cut(s) 53, 109, 385, 840
PleI GAGTC 1 cut(s) 286
PluTI GGCGCC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 286
Psp124BI GAGCTC 2 cut(s) 162, 376
PspFI CCCAGC 2 cut(s) 87, 604
PspN4I GGNNCC 1 cut(s) 106
PspPI GGNCC 1 cut(s) 515
PsuI RGATCY 1 cut(s) 612
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 2 cut(s) 444, 847
SacI GAGCTC 2 cut(s) 162, 376
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 4 cut(s) 114, 684, 717, 948
SatI GCNGC 4 cut(s) 52, 108, 384, 839
Sau3AI GATC 3 cut(s) 234, 520, 612
Sau96I GGNCC 1 cut(s) 515
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 239, 240
SduI GDGCHC 2 cut(s) 162, 376
SfaNI GCATC 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 627
SfoI GGCGCC 1 cut(s) 106
SinI GGWCC 1 cut(s) 515
SmaI CCCGGG 1 cut(s) 240
SmiMI CAYNNNNRTG 2 cut(s) 444, 847
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 4 cut(s) 302, 734, 758, 866
SsiI CCGC 5 cut(s) 51, 54, 62, 108, 804
SspDI GGCGCC 1 cut(s) 104
SspI AATATT 2 cut(s) 185, 700
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 2 cut(s) 162, 376
StyD4I CCNGG 2 cut(s) 237, 238
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 2 cut(s) 74, 434
TaqI TCGA 3 cut(s) 69, 555, 741
TasI AATT 4 cut(s) 302, 734, 758, 866
TatI WGTACW 1 cut(s) 429
TauI GCSGC 2 cut(s) 54, 110
TfiI GAWTC 3 cut(s) 97, 327, 751
Tru1I TTAA 4 cut(s) 114, 684, 717, 948
Tru9I TTAA 4 cut(s) 114, 684, 717, 948
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 2 cut(s) 437, 893
TseI GCWGC 2 cut(s) 383, 838
Tsp45I GTSAC 2 cut(s) 437, 893
TspDTI ATGAA 3 cut(s) 207, 727, 879
TspMI CCCGGG 1 cut(s) 238
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 515
XapI RAATTY 2 cut(s) 758, 866
XbaI TCTAGA 1 cut(s) 251
XmaI CCCGGG 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.