Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012188

Physical Location & Seq

Genomic Coordinates Reverse (-)

3041614 .. 3043674

Gene Structure 2061 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1043200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGGGATTGAAGAAGGCCTTTACCTCGTCGTTGGAGGATGTAATAGAGAAAGCAGGGGGCTGTGCTGTGGTGGACGGAGGCTTCGCCACCCAACTGGAGAGGCACGGCGCTGCCATCAACGACCCTCTCTGGAGCGCCGTCTGCCTCATCAAACAACCCGACCTCATCAAACGGGTTCACTTGGACTACTTGGAAGCCGGCGCTGATATTCTCATCACTTCATCTTACCAGGCCACAATACCCGGATTTTTGTCCAGAGGACTCTCCATTGAACAAGGGGAGTTGTTGCTGAAGAAAAGTGTGAAACTGGCTGTCGAAGCCCGAAACAGTTTCTGGGATGCTCTGAAAGTGACACCCGATCATCGTTATAACCGAGCTCTGGTTGCTGCCTCCATTGGAAGCTACGGAGCTTATCTCGCTGACGGCTCAGAATATAGCGGGTGTTATGGACCCCGTGTGGATGTTGATAAGCTGAAGGATTTCCACCGGCGCAGATTGCAAGTTCTTGTCGAAGCAGGTCCTGATTTACTTGCATTTGAGACCATTCCTAATAAACTTGAAGCTCAGGCCTGCGTCGAGCTGCTGGAAGAACAGAACGTCCAGATTCCATCTTGGATATGTTTCAGCTCTGTGGATGGTGAAAATGCCCCATCTGGGGAGGGTTTTACGGAATGCCTTGAGGTGATAAACAAAAGTAACAAAATACATGCTGTGGGAATAAATTGCACACCTCCTCATCTGATTCAATCTCTCATCTGCAAGTTTAAGGAGTTAACCAGTAAAGCCATTATTGTGTACCCTAATAGTGGTGAGATATGGGACGGCAAAGCTAAGAGATGGCTGCCTGCCAAGTGTTTTGATGACGAAAACTTTGAATGCTTTGCAACAATCTGGCGCGATTCGGGTGCTAAACTCATCGGAGGATGTTGTCGGACAACGCCTTCCACTGTTCAAGCCATTTCCAAGGTTCTAAAAGGACAGTCCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0006082 GO:0006520 GO:0006555 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008172 GO:0008652 GO:0008757 GO:0008898 GO:0009058 GO:0009066 GO:0009067 GO:0009086 GO:0009987 GO:0016053 GO:0016740 GO:0016741 GO:0019752 GO:0032259 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko01110 map00270 map01100 map01110

Pfam Domains

Protein Families

S-methyl_trans

Protein Analysis

330

Amino Acids

35.97

Weight (kDa)

5.93

Isoelectric Point (pI)

35.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S-methyl_trans	PF02574	23 - 323	2.4e-77	Homocysteine S-methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012188)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25900 AT3G25900 AT3G25900
fragaria_vesca	FvH4_4g27410 FvH4_4g27410
malus_domestica	MD16G1043200.v1.1
prunus_persica	Prupe.1G310700_v2.0.a1
pyrus_communis	pycom16g03730
rosa_chinensis	RchiOBHm_Chr4g0435231
rosa_laevigata	RLG00000006608
rosa_multiflora	Rmu_ssc0000339.1_g000027
rosa_roxburghii	Rroxscaffold_5G00376440
rosa_rugosa	Rorug04G0283300 Rorug04G0283400
rosa_samantha	Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana	Rw4G029560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	369
Acc36I	ACCTGC	1 cut(s)	506
AccII	CGCG	1 cut(s)	897
AciI	CCGC	1 cut(s)	438
AcuI	CTGAAG	2 cut(s)	311, 494
AfaI	GTAC	1 cut(s)	797
AfiI	CCNNNNNNNGG	4 cut(s)	379, 654, 655, 806
AgsI	TTSAA	6 cut(s)	10, 272, 560, 746, 875, 953
AjnI	CCWGG	1 cut(s)	228
AloI	GAACNNNNNNTCC	2 cut(s)	582, 614
AluBI	AGCT	8 cut(s)	377, 402, 410, 472, 563, 580, 627, 830
AluI	AGCT	8 cut(s)	377, 402, 410, 472, 563, 580, 627, 830
Alw21I	GWGCWC	1 cut(s)	379
Alw26I	GTCTC	1 cut(s)	533
AlwNI	CAGNNNCTG	2 cut(s)	333, 521
AoxI	GGCC	3 cut(s)	15, 231, 567
ApeKI	GCWGC	4 cut(s)	110, 386, 580, 841
ArsI	GACNNNNNNTTYG	4 cut(s)	65, 97, 854, 886
Asp700I	GAANNNNTTC	1 cut(s)	479
AspLEI	GCGC	5 cut(s)	110, 137, 203, 492, 897
AspS9I	GGNCC	2 cut(s)	449, 518
AsuC2I	CCSGG	1 cut(s)	243
AsuHPI	GGTGA	3 cut(s)	650, 694, 821
AvaII	GGWCC	2 cut(s)	449, 518
BanII	GRGCYC	1 cut(s)	379
Bbv12I	GWGCWC	1 cut(s)	379
BbvI	GCAGC	4 cut(s)	97, 373, 567, 828
BccI	CCATC	5 cut(s)	122, 616, 629, 658, 831
BceAI	ACGGC	4 cut(s)	121, 122, 439, 838
BcgI	CGANNNNNNTGC	2 cut(s)	887, 921
BciT130I	CCWGG	1 cut(s)	230
BcnI	CCSGG	1 cut(s)	243
BcoDI	GTCTC	1 cut(s)	533
BfoI	RGCGCY	3 cut(s)	111, 138, 204
BfuAI	ACCTGC	1 cut(s)	506
BisI	GCNGC	4 cut(s)	111, 387, 581, 842
BlsI	GCNGC	4 cut(s)	112, 388, 582, 843
Bme1390I	CCNGG	2 cut(s)	230, 243
Bme18I	GGWCC	2 cut(s)	449, 518
BmgT120I	GGNCC	2 cut(s)	449, 518
BmiI	GGNNCC	1 cut(s)	451
BmrFI	CCNGG	2 cut(s)	230, 243
BmsI	GCATC	1 cut(s)	328
BplI	GAGNNNNNCTC	2 cut(s)	399, 431
BpmI	CTGGAG	2 cut(s)	116, 151
Bpu10I	CCTNAGC	1 cut(s)	564
BpuEI	CTTGAG	1 cut(s)	698
BpuMI	CCSGG	1 cut(s)	243
BsaI	GGTCTC	1 cut(s)	533
BsaJI	CCNNGG	1 cut(s)	963
Bsc4I	CCNNNNNNNGG	4 cut(s)	379, 654, 655, 806
Bse118I	RCCGGY	2 cut(s)	197, 486
Bse1I	ACTGG	3 cut(s)	99, 312, 777
BseBI	CCWGG	1 cut(s)	230
BseDI	CCNNGG	1 cut(s)	963
BseGI	GGATG	5 cut(s)	43, 343, 466, 640, 929
BseLI	CCNNNNNNNGG	4 cut(s)	379, 654, 655, 806
BseMII	CTCAG	2 cut(s)	441, 578
BseNI	ACTGG	3 cut(s)	99, 312, 777
BseRI	GAGGAG	1 cut(s)	723
BseXI	GCAGC	4 cut(s)	97, 373, 567, 828
Bsh1236I	CGCG	1 cut(s)	897
BshFI	GGCC	3 cut(s)	17, 233, 569
BsiHKAI	GWGCWC	1 cut(s)	379
BsiSI	CCGG	3 cut(s)	198, 243, 487
BslFI	GGGAC	2 cut(s)	833, 967
BslI	CCNNNNNNNGG	4 cut(s)	379, 654, 655, 806
BsmAI	GTCTC	1 cut(s)	533
BsmFI	GGGAC	2 cut(s)	833, 967
BsmI	GAATGC	2 cut(s)	677, 881
BsnI	GGCC	3 cut(s)	17, 233, 569
Bso31I	GGTCTC	1 cut(s)	533
Bsp1286I	GDGCHC	1 cut(s)	379
Bsp143I	GATC	1 cut(s)	358
BspACI	CCGC	1 cut(s)	438
BspANI	GGCC	3 cut(s)	17, 233, 569
BspCNI	CTCAG	2 cut(s)	440, 577
BspFNI	CGCG	1 cut(s)	897
BspLI	GGNNCC	1 cut(s)	451
BspMI	ACCTGC	1 cut(s)	506
BspTNI	GGTCTC	1 cut(s)	533
BsrFI	RCCGGY	2 cut(s)	197, 486
BsrI	ACTGG	3 cut(s)	99, 312, 777
BssAI	RCCGGY	2 cut(s)	197, 486
BssECI	CCNNGG	1 cut(s)	963
BssMI	GATC	1 cut(s)	358
BssT1I	CCWWGG	1 cut(s)	963
Bst2UI	CCWGG	1 cut(s)	230
Bst4CI	ACNGT	3 cut(s)	329, 949, 981
BstC8I	GCNNGC	3 cut(s)	199, 571, 846
BstDEI	CTNAG	3 cut(s)	427, 564, 831
BstF5I	GGATG	5 cut(s)	43, 343, 466, 640, 929
BstFNI	CGCG	1 cut(s)	897
BstH2I	RGCGCY	3 cut(s)	111, 138, 204
BstHHI	GCGC	5 cut(s)	110, 137, 203, 492, 897
BstKTI	GATC	1 cut(s)	361
BstMAI	GTCTC	1 cut(s)	533
BstMBI	GATC	1 cut(s)	358
BstMWI	GCNNNNNNNGC	5 cut(s)	141, 317, 383, 416, 496
BstNI	CCWGG	1 cut(s)	230
BstNSI	RCATGY	1 cut(s)	710
BstSCI	CCNGG	2 cut(s)	228, 241
BstUI	CGCG	1 cut(s)	897
BstV1I	GCAGC	4 cut(s)	97, 373, 567, 828
BstXI	CCANNNNNNTGG	1 cut(s)	94
BsuRI	GGCC	3 cut(s)	17, 233, 569
BtsCI	GGATG	5 cut(s)	43, 343, 466, 640, 929
BtsIMutI	CAGTG	1 cut(s)	945
BveI	ACCTGC	1 cut(s)	506
Cac8I	GCNNGC	3 cut(s)	199, 571, 846
CaiI	CAGNNNCTG	2 cut(s)	333, 521
CfoI	GCGC	5 cut(s)	110, 137, 203, 492, 897
Cfr10I	RCCGGY	2 cut(s)	197, 486
Cfr13I	GGNCC	2 cut(s)	449, 518
CseI	GACGC	1 cut(s)	562
Csp6I	GTAC	1 cut(s)	796
CviAII	CATG	1 cut(s)	707
CviQI	GTAC	1 cut(s)	796
DdeI	CTNAG	3 cut(s)	427, 564, 831
DpnI	GATC	1 cut(s)	360
DpnII	GATC	1 cut(s)	358
Ecl136II	GAGCTC	1 cut(s)	377
Eco130I	CCWWGG	1 cut(s)	963
Eco147I	AGGCCT	2 cut(s)	17, 569
Eco24I	GRGCYC	1 cut(s)	379
Eco31I	GGTCTC	1 cut(s)	533
Eco47I	GGWCC	2 cut(s)	449, 518
Eco53kI	GAGCTC	1 cut(s)	377
Eco57I	CTGAAG	2 cut(s)	311, 494
EcoICRI	GAGCTC	1 cut(s)	377
EcoO109I	RGGNCCY	1 cut(s)	518
EcoRII	CCWGG	1 cut(s)	228
EcoT14I	CCWWGG	1 cut(s)	963
EcoT38I	GRGCYC	1 cut(s)	379
ErhI	CCWWGG	1 cut(s)	963
FaeI	CATG	1 cut(s)	710
FaiI	YATR	6 cut(s)	369, 435, 447, 619, 708, 817
FalI	AAGNNNNNCTT	1 cut(s)	34
FaqI	GGGAC	2 cut(s)	833, 967
FatI	CATG	1 cut(s)	706
FauI	CCCGC	1 cut(s)	431
Fnu4HI	GCNGC	4 cut(s)	111, 387, 581, 842
FokI	GGATG	5 cut(s)	50, 350, 473, 647, 936
FriOI	GRGCYC	1 cut(s)	379
Fsp4HI	GCNGC	4 cut(s)	111, 387, 581, 842
GlaI	GCGC	5 cut(s)	109, 136, 202, 491, 896
GluI	GCNGC	4 cut(s)	111, 387, 581, 842
GsuI	CTGGAG	2 cut(s)	116, 151
HaeII	RGCGCY	3 cut(s)	111, 138, 204
HaeIII	GGCC	3 cut(s)	17, 233, 569
HapII	CCGG	3 cut(s)	198, 243, 487
HgaI	GACGC	1 cut(s)	562
HhaI	GCGC	5 cut(s)	110, 137, 203, 492, 897
Hin1II	CATG	1 cut(s)	710
Hin6I	GCGC	5 cut(s)	108, 135, 201, 490, 895
HinP1I	GCGC	5 cut(s)	108, 135, 201, 490, 895
HincII	GTYRAC	1 cut(s)	774
HindII	GTYRAC	1 cut(s)	774
HinfI	GANTC	4 cut(s)	261, 604, 742, 899
HpaI	GTTAAC	1 cut(s)	774
HpaII	CCGG	3 cut(s)	198, 243, 487
HphI	GGTGA	3 cut(s)	650, 694, 821
Hpy166II	GTNNAC	4 cut(s)	73, 178, 774, 796
Hpy188I	TCNGA	5 cut(s)	345, 430, 741, 920, 933
Hpy188III	TCNNGA	4 cut(s)	130, 255, 521, 601
Hpy8I	GTNNAC	4 cut(s)	73, 178, 774, 796
Hpy99I	CGWCG	2 cut(s)	31, 578
HpyAV	CCTTC	3 cut(s)	7, 469, 951
HpyCH4III	ACNGT	3 cut(s)	329, 949, 981
HpyCH4IV	ACGT	1 cut(s)	597
HpyCH4V	TGCA	5 cut(s)	499, 533, 726, 759, 884
HpyF10VI	GCNNNNNNNGC	5 cut(s)	141, 317, 383, 416, 496
HpyF3I	CTNAG	3 cut(s)	427, 564, 831
HpySE526I	ACGT	1 cut(s)	597
Hsp92II	CATG	1 cut(s)	710
HspAI	GCGC	5 cut(s)	108, 135, 201, 490, 895
KroI	GCCGGC	1 cut(s)	197
KroNI	GCCGGC	1 cut(s)	199
KspAI	GTTAAC	1 cut(s)	774
Kzo9I	GATC	1 cut(s)	358
LmnI	GCTCC	2 cut(s)	132, 407
Lsp1109I	GCAGC	4 cut(s)	97, 373, 567, 828
LweI	GCATC	1 cut(s)	328
MaeII	ACGT	1 cut(s)	597
MaeIII	GTNAC	2 cut(s)	349, 695
MalI	GATC	1 cut(s)	360
MboI	GATC	1 cut(s)	358
MboII	GAAGA	3 cut(s)	22, 304, 599
MhlI	GDGCHC	1 cut(s)	379
MluCI	AATT	1 cut(s)	721
MlyI	GAGTC	1 cut(s)	255
MmeI	TCCRAC	2 cut(s)	12, 911
MroNI	GCCGGC	1 cut(s)	197
MroXI	GAANNNNTTC	1 cut(s)	479
MseI	TTAA	2 cut(s)	765, 773
MslI	CAYNNNNRTG	1 cut(s)	791
MspI	CCGG	3 cut(s)	198, 243, 487
MspR9I	CCNGG	2 cut(s)	230, 243
Mva1269I	GAATGC	1 cut(s)	677
MvaI	CCWGG	1 cut(s)	230
MvnI	CGCG	1 cut(s)	897
MwoI	GCNNNNNNNGC	5 cut(s)	141, 317, 383, 416, 496
NaeI	GCCGGC	1 cut(s)	199
NciI	CCSGG	1 cut(s)	243
NdeII	GATC	1 cut(s)	358
NgoMIV	GCCGGC	1 cut(s)	197
NlaIII	CATG	1 cut(s)	710
NlaIV	GGNNCC	1 cut(s)	451
NmuCI	GTSAC	1 cut(s)	349
NspI	RCATGY	1 cut(s)	710
PceI	AGGCCT	2 cut(s)	17, 569
PcsI	WCGNNNNNNNCGW	1 cut(s)	370
PctI	GAATGC	2 cut(s)	677, 881
PdiI	GCCGGC	1 cut(s)	199
PdmI	GAANNNNTTC	1 cut(s)	479
PfeI	GAWTC	3 cut(s)	604, 742, 899
PkrI	GCNGC	4 cut(s)	112, 388, 582, 843
PleI	GAGTC	1 cut(s)	255
PpsI	GAGTC	1 cut(s)	255
PpuMI	RGGWCCY	1 cut(s)	518
PsiI	TTATAA	1 cut(s)	369
Psp124BI	GAGCTC	1 cut(s)	379
Psp5II	RGGWCCY	1 cut(s)	518
Psp6I	CCWGG	1 cut(s)	228
PspGI	CCWGG	1 cut(s)	228
PspN4I	GGNNCC	1 cut(s)	451
PspPI	GGNCC	2 cut(s)	449, 518
PspPPI	RGGWCCY	1 cut(s)	518
PstNI	CAGNNNCTG	2 cut(s)	333, 521
RsaI	GTAC	1 cut(s)	797
RsaNI	GTAC	1 cut(s)	796
RseI	CAYNNNNRTG	1 cut(s)	791
SacI	GAGCTC	1 cut(s)	379
SaqAI	TTAA	2 cut(s)	765, 773
SatI	GCNGC	4 cut(s)	111, 387, 581, 842
Sau3AI	GATC	1 cut(s)	358
Sau96I	GGNCC	2 cut(s)	449, 518
SchI	GAGTC	1 cut(s)	255
ScrFI	CCNGG	2 cut(s)	230, 243
SduI	GDGCHC	1 cut(s)	379
SfaNI	GCATC	1 cut(s)	328
SgrAI	CRCCGGYG	1 cut(s)	486
SinI	GGWCC	2 cut(s)	449, 518
SmiMI	CAYNNNNRTG	1 cut(s)	791
SmlI	CTYRAG	1 cut(s)	677
SmoI	CTYRAG	1 cut(s)	677
Sse9I	AATT	1 cut(s)	721
SseBI	AGGCCT	2 cut(s)	17, 569
SsiI	CCGC	1 cut(s)	438
SstI	GAGCTC	1 cut(s)	379
StuI	AGGCCT	2 cut(s)	17, 569
StyD4I	CCNGG	2 cut(s)	228, 241
StyI	CCWWGG	1 cut(s)	963
TaaI	ACNGT	3 cut(s)	329, 949, 981
TaiI	ACGT	1 cut(s)	600
TaqI	TCGA	3 cut(s)	315, 510, 576
TasI	AATT	1 cut(s)	721
TfiI	GAWTC	3 cut(s)	604, 742, 899
Tru1I	TTAA	2 cut(s)	765, 773
Tru9I	TTAA	2 cut(s)	765, 773
TscAI	CASTG	1 cut(s)	952
TseFI	GTSAC	1 cut(s)	349
TseI	GCWGC	4 cut(s)	110, 386, 580, 841
Tsp45I	GTSAC	1 cut(s)	349
TspDTI	ATGAA	1 cut(s)	210
TspGWI	ACGGA	3 cut(s)	90, 420, 683
TspRI	CASTG	1 cut(s)	952
VpaK11BI	GGWCC	2 cut(s)	449, 518
XceI	RCATGY	1 cut(s)	710

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.