FvH4_4g27410

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012188
Physical Location & Seq
Reverse (-)
28417032 .. 28419706
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27410.t1

Sequence Viewer

Length: 1002 bp
ATGGTCGGAAAGAAGAAGGAAACGTCGATGACGACGACCTTGGAAGACTTGATAGAGAAAGCAGGAGGTTGCGCTGTCGTGGACGGAGGGTTCGCAACACAGTTGGAGAAACACGGCGCTGCTATCAACGACACTCTCTGGAGCGCCGTTTGCCTCATCAAGGACCCCGATCTCATCAAGAGGGTCCACTTGGAATACCTGGAAGCCGGCGCCGATGTTCTGGTCACTTCGTCTTACCAGGCAACAATTCCTGGATTTTTGTCCAAGGGACTGTCCATTGAAGAAGGGGAGTCATTGCTGAAGAAGAGTGTGACACTAGCTGTCGAAGCTCGGAATAGTTTCTGGGAGGCAGTGAAAGTGAATCCCGGGCATCATCACTATAACAGGGCTTTGGTTGCTGCCTCTATCGGAAGCTATGGCGCTTATCTTGCTGATGGTTCTGAATACAGTGGGGATTATGGACCGAATGTGGATTTGGATAAGCTGAAGGATTTTCACCGCCGTAGATTGCAAGTTCTTGTGGAAGCAGGTCCAGATTTACTTGCATTTGAGACCATTCCTAATAAGCTCGAAGCTCAGGCCTGCATTGAGCTGCTTGAAGAAGAAAACATTCAACTGCCATCTTGGATATGTTTCAGCTCTGTAGATGGTGAGCATGCCCCATCTGGAGAGAGCTTCAAAGAATGCCTTGACATAATAAACAGAAGTAACAAAGTGAATGCAGTGGGGATAAACTGTGCACCTCCTCATTTTACTGAAAGTCTCATCTGCAAATTTCAGCAGTTAACCAGTAAGGCAATTGTCGTGTACCCTAATAGTGGTGAGATATGGGATGGCAAAGCTAAGAGATGGTTGCCTTCCAAGTGCTTCGATGATGACAAGTTTGAATGCTTTGCGACAAGATGGCGCGATTCAGGAGCTAAGCTCATTGGAGGCTGTTGCCGGACAACACCATCAACCATTCAAGCAATTTCTAAGGTTCTAAAGGGGAAATCCCAGTGA

Protein Analysis

334

Amino Acids

36.43

Weight (kDa)

5.69

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 26 - 327 3.2e-77 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 209
AccII CGCG 1 cut(s) 909
AciI CCGC 1 cut(s) 499
AcsI RAATTY 1 cut(s) 773
AcuI CTGAAG 2 cut(s) 320, 506
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 1 cut(s) 809
AfiI CCNNNNNNNGG 2 cut(s) 160, 818
AgsI TTSAA 6 cut(s) 281, 599, 614, 679, 887, 965
AjnI CCWGG 3 cut(s) 198, 237, 250
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 2 cut(s) 545, 767
Alw44I GTGCAC 1 cut(s) 738
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 1 cut(s) 579
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 3 cut(s) 119, 398, 592
ApoI RAATTY 1 cut(s) 773
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp700I GAANNNNTTC 2 cut(s) 338, 609
AspLEI GCGC 6 cut(s) 74, 119, 146, 212, 422, 909
AspS9I GGNCC 4 cut(s) 163, 184, 461, 530
AsuC2I CCSGG 2 cut(s) 366, 367
AsuHPI GGTGA 3 cut(s) 488, 662, 833
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 4 cut(s) 163, 184, 461, 530
BaeGI GKGCMC 1 cut(s) 742
BanI GGYRCC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 3 cut(s) 106, 385, 579
BccI CCATC 8 cut(s) 428, 628, 641, 670, 827, 843, 897, 961
BceAI ACGGC 3 cut(s) 130, 131, 486
BciT130I CCWGG 3 cut(s) 200, 239, 252
BcnI CCSGG 2 cut(s) 366, 367
BcoDI GTCTC 2 cut(s) 545, 767
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 642
BfoI RGCGCY 4 cut(s) 120, 147, 213, 423
BfuAI ACCTGC 1 cut(s) 518
BisI GCNGC 3 cut(s) 120, 399, 593
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 3 cut(s) 121, 400, 594
Bme1390I CCNGG 5 cut(s) 200, 239, 252, 366, 367
Bme18I GGWCC 4 cut(s) 163, 184, 461, 530
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 4 cut(s) 163, 184, 461, 530
BmiI GGNNCC 3 cut(s) 165, 185, 211
BmrFI CCNGG 5 cut(s) 200, 239, 252, 366, 367
BmrI ACTGGG 1 cut(s) 991
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 991
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 2 cut(s) 909, 941
BpmI CTGGAG 2 cut(s) 160, 687
Bpu10I CCTNAGC 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 921
BpuMI CCSGG 2 cut(s) 366, 367
BsaHI GRCGYC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 545
BsaJI CCNNGG 3 cut(s) 39, 264, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 818
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 2 cut(s) 789, 997
Bse3DI GCAATG 1 cut(s) 293
BseBI CCWGG 3 cut(s) 200, 239, 252
BseDI CCNNGG 3 cut(s) 39, 264, 365
BseGI GGATG 1 cut(s) 838
BseLI CCNNNNNNNGG 2 cut(s) 160, 818
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 590
BseNI ACTGG 2 cut(s) 789, 997
BseRI GAGGAG 1 cut(s) 735
BseSI GKGCMC 1 cut(s) 742
BseXI GCAGC 3 cut(s) 106, 385, 579
Bsh1236I CGCG 1 cut(s) 909
BshFI GGCC 1 cut(s) 581
BshNI GGYRCC 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 742
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 3 cut(s) 207, 366, 943
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 160, 818
BsmAI GTCTC 2 cut(s) 545, 767
BsmFI GGGAC 1 cut(s) 282
BsmI GAATGC 3 cut(s) 689, 724, 893
BsnI GGCC 1 cut(s) 581
Bso31I GGTCTC 1 cut(s) 545
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 742
Bsp143I GATC 1 cut(s) 169
Bsp1720I GCTNAGC 1 cut(s) 921
BspACI CCGC 1 cut(s) 499
BspANI GGCC 1 cut(s) 581
BspCNI CTCAG 1 cut(s) 589
BspFNI CGCG 1 cut(s) 909
BspLI GGNNCC 3 cut(s) 165, 185, 211
BspMI ACCTGC 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 2 cut(s) 789, 997
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 3 cut(s) 39, 264, 365
BssMI GATC 1 cut(s) 169
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 2 cut(s) 39, 264
Bst2UI CCWGG 3 cut(s) 200, 239, 252
Bst4CI ACNGT 4 cut(s) 102, 273, 449, 737
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 208, 583, 657
BstDEI CTNAG 4 cut(s) 576, 843, 921, 975
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 1 cut(s) 909
BstH2I RGCGCY 4 cut(s) 120, 147, 213, 423
BstHHI GCGC 6 cut(s) 74, 119, 146, 212, 422, 909
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 2 cut(s) 545, 767
BstMBI GATC 1 cut(s) 169
BstMWI GCNNNNNNNGC 4 cut(s) 150, 326, 395, 428
BstNI CCWGG 3 cut(s) 200, 239, 252
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 5 cut(s) 198, 237, 250, 364, 365
BstSFI CTRYAG 1 cut(s) 642
BstSLI GKGCMC 1 cut(s) 742
BstUI CGCG 1 cut(s) 909
BstV1I GCAGC 3 cut(s) 106, 385, 579
BstV2I GAAGAC 1 cut(s) 51
BsuRI GGCC 1 cut(s) 581
BtsCI GGATG 1 cut(s) 838
BtsI GCAGTG 2 cut(s) 357, 729
BtsIMutI CAGTG 3 cut(s) 357, 454, 729
BveI ACCTGC 1 cut(s) 518
Cac8I GCNNGC 3 cut(s) 208, 583, 657
CfoI GCGC 6 cut(s) 74, 119, 146, 212, 422, 909
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 4 cut(s) 163, 184, 461, 530
Cfr9I CCCGGG 1 cut(s) 365
Csp6I GTAC 1 cut(s) 808
CviAII CATG 1 cut(s) 656
CviQI GTAC 1 cut(s) 808
DdeI CTNAG 4 cut(s) 576, 843, 921, 975
DinI GGCGCC 1 cut(s) 211
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
Eco130I CCWWGG 2 cut(s) 39, 264
Eco147I AGGCCT 1 cut(s) 581
Eco31I GGTCTC 1 cut(s) 545
Eco47I GGWCC 4 cut(s) 163, 184, 461, 530
Eco57I CTGAAG 2 cut(s) 320, 506
Eco88I CYCGRG 1 cut(s) 365
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 3 cut(s) 198, 237, 250
EcoT14I CCWWGG 2 cut(s) 39, 264
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 2 cut(s) 39, 264
FaeI CATG 1 cut(s) 659
FaiI YATR 7 cut(s) 381, 417, 459, 631, 657, 695, 829
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FaqI GGGAC 1 cut(s) 282
FatI CATG 1 cut(s) 655
Fnu4HI GCNGC 3 cut(s) 120, 399, 593
FokI GGATG 1 cut(s) 845
Fsp4HI GCNGC 3 cut(s) 120, 399, 593
FspBI CTAG 1 cut(s) 317
GlaI GCGC 6 cut(s) 73, 118, 145, 211, 421, 908
GluI GCNGC 3 cut(s) 120, 399, 593
GsuI CTGGAG 2 cut(s) 160, 687
HaeII RGCGCY 4 cut(s) 120, 147, 213, 423
HaeIII GGCC 1 cut(s) 581
HapII CCGG 3 cut(s) 207, 366, 943
HhaI GCGC 6 cut(s) 74, 119, 146, 212, 422, 909
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 1 cut(s) 659
Hin6I GCGC 6 cut(s) 72, 117, 144, 210, 420, 907
HinP1I GCGC 6 cut(s) 72, 117, 144, 210, 420, 907
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HinfI GANTC 3 cut(s) 290, 361, 911
HpaI GTTAAC 1 cut(s) 786
HpaII CCGG 3 cut(s) 207, 366, 943
HphI GGTGA 3 cut(s) 488, 662, 833
Hpy166II GTNNAC 5 cut(s) 82, 187, 740, 786, 808
Hpy188I TCNGA 4 cut(s) 8, 333, 410, 442
Hpy188III TCNNGA 5 cut(s) 139, 178, 533, 666, 915
Hpy8I GTNNAC 5 cut(s) 82, 187, 740, 786, 808
Hpy99I CGWCG 2 cut(s) 28, 37
HpyAV CCTTC 4 cut(s) 10, 278, 481, 867
HpyCH4III ACNGT 4 cut(s) 102, 273, 449, 737
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 6 cut(s) 511, 545, 585, 722, 740, 771
HpyF10VI GCNNNNNNNGC 4 cut(s) 150, 326, 395, 428
HpyF3I CTNAG 4 cut(s) 576, 843, 921, 975
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 1 cut(s) 659
HspAI GCGC 6 cut(s) 72, 117, 144, 210, 420, 907
KasI GGCGCC 1 cut(s) 209
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
KspAI GTTAAC 1 cut(s) 786
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 2 cut(s) 141, 917
Lsp1109I GCAGC 3 cut(s) 106, 385, 579
LweI GCATC 1 cut(s) 379
MaeI CTAG 1 cut(s) 317
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 3 cut(s) 223, 310, 707
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 7 cut(s) 25, 56, 293, 313, 316, 611, 614
MfeI CAATTG 1 cut(s) 798
MhlI GDGCHC 1 cut(s) 742
MluCI AATT 4 cut(s) 246, 773, 798, 969
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 9 cut(s) 59, 80, 164, 174, 340, 412, 753, 756, 926
MroNI GCCGGC 1 cut(s) 206
MroXI GAANNNNTTC 2 cut(s) 338, 609
MseI TTAA 1 cut(s) 785
MspI CCGG 3 cut(s) 207, 366, 943
MspR9I CCNGG 5 cut(s) 200, 239, 252, 366, 367
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 3 cut(s) 689, 724, 893
MvaI CCWGG 3 cut(s) 200, 239, 252
MvnI CGCG 1 cut(s) 909
MwoI GCNNNNNNNGC 4 cut(s) 150, 326, 395, 428
NaeI GCCGGC 1 cut(s) 208
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 2 cut(s) 366, 367
NdeII GATC 1 cut(s) 169
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 1 cut(s) 659
NlaIV GGNNCC 3 cut(s) 165, 185, 211
NmuCI GTSAC 2 cut(s) 223, 310
NspI RCATGY 1 cut(s) 659
PaeI GCATGC 1 cut(s) 659
PceI AGGCCT 1 cut(s) 581
PcsI WCGNNNNNNNCGW 2 cut(s) 29, 32
PctI GAATGC 3 cut(s) 689, 724, 893
PdiI GCCGGC 1 cut(s) 208
PdmI GAANNNNTTC 2 cut(s) 338, 609
PfeI GAWTC 2 cut(s) 361, 911
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 3 cut(s) 121, 400, 594
PleI GAGTC 1 cut(s) 298
PluTI GGCGCC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 298
PpuMI RGGWCCY 1 cut(s) 163
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 3 cut(s) 198, 237, 250
PspGI CCWGG 3 cut(s) 198, 237, 250
PspN4I GGNNCC 3 cut(s) 165, 185, 211
PspPI GGNCC 4 cut(s) 163, 184, 461, 530
PspPPI RGGWCCY 1 cut(s) 163
RsaI GTAC 1 cut(s) 809
RsaNI GTAC 1 cut(s) 808
SaqAI TTAA 1 cut(s) 785
SatI GCNGC 3 cut(s) 120, 399, 593
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 4 cut(s) 163, 184, 461, 530
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 5 cut(s) 200, 239, 252, 366, 367
SduI GDGCHC 1 cut(s) 742
SfaNI GCATC 1 cut(s) 379
SfcI CTRYAG 1 cut(s) 642
SfoI GGCGCC 1 cut(s) 211
SinI GGWCC 4 cut(s) 163, 184, 461, 530
SmaI CCCGGG 1 cut(s) 367
SphI GCATGC 1 cut(s) 659
Sse9I AATT 4 cut(s) 246, 773, 798, 969
SseBI AGGCCT 1 cut(s) 581
SsiI CCGC 1 cut(s) 499
SspDI GGCGCC 1 cut(s) 209
SspMI CTAG 1 cut(s) 317
StuI AGGCCT 1 cut(s) 581
StyD4I CCNGG 5 cut(s) 198, 237, 250, 364, 365
StyI CCWWGG 2 cut(s) 39, 264
TaaI ACNGT 4 cut(s) 102, 273, 449, 737
TaiI ACGT 1 cut(s) 26
TaqI TCGA 4 cut(s) 26, 324, 570, 870
TaqII GACCGA 1 cut(s) 478
TasI AATT 4 cut(s) 246, 773, 798, 969
TfiI GAWTC 2 cut(s) 361, 911
Tru1I TTAA 1 cut(s) 785
Tru9I TTAA 1 cut(s) 785
TscAI CASTG 3 cut(s) 357, 454, 729
TseFI GTSAC 2 cut(s) 223, 310
TseI GCWGC 3 cut(s) 119, 398, 592
Tsp45I GTSAC 2 cut(s) 223, 310
TspGWI ACGGA 1 cut(s) 99
TspMI CCCGGG 1 cut(s) 365
TspRI CASTG 3 cut(s) 357, 454, 729
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 4 cut(s) 163, 184, 461, 530
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 773
XceI RCATGY 1 cut(s) 659
XmaI CCCGGG 1 cut(s) 365
XmnI GAANNNNTTC 2 cut(s) 338, 609
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.