Rorug04G0283400

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012188
Physical Location & Seq
Forward (+)
45702727 .. 45704263
1537 bp
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UTR
Exon/CDS
Intron
Rorug04G0283400.1

Sequence Viewer

Length: 483 bp
ATGGGTGTCTTCACTTATGAAACTGAGTTCACCTCCGTCATCCCACCAGCTAGATTATTCAAGGCCTTTATCCTTGATGCCGACACTCTCATCCCAAAGATTGCTCCACAAGCAGTCAAGGGGGCTGAAATTGTAGAAGGAGATGGAGGCGTAGGAACCATCAAGAAGATCACCTTCGGTGAAGGCAGCCAGTTCGGCTCTGTAACTCACAAGATTGATGGGATTGACAAAGATAACTTTGTCTACAACTACAGTTTGATCGAAGGAGATGCCCTGTCCGACAAGATTGAGAAGATCTCTTACGAGACCAAATTGGTGGCTTCTTCCGATGGAGGATCCGTCATCAAGAGCACAAGCAACTACCACACCAAAGGTGATGTGGAGATCAAAGAAGAGCACATTAAGGCTGGCAAAGAGAAGGCATCCCACCTCTTCAAGCTAGTTGAAGAGTACCTCCTGGCCAATCCTAATGAGTACTGTTAA

Protein Analysis

160

Amino Acids

17.47

Weight (kDa)

5.11

Isoelectric Point (pI)

18.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 155 1.4e-24 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 243
AclWI GGATC 2 cut(s) 330, 343
AcoI YGGCCR 1 cut(s) 459
AfaI GTAC 2 cut(s) 452, 476
AgsI TTSAA 3 cut(s) 61, 436, 446
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 2 cut(s) 50, 439
AluI AGCT 2 cut(s) 50, 439
Alw21I GWGCWC 2 cut(s) 353, 399
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 2 cut(s) 330, 343
AoxI GGCC 2 cut(s) 63, 459
ApeKI GCWGC 1 cut(s) 186
AsuHPI GGTGA 4 cut(s) 22, 163, 191, 386
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 1 cut(s) 335
BarI GAAGNNNNNNTAC 2 cut(s) 284, 316
Bbv12I GWGCWC 2 cut(s) 353, 399
BbvI GCAGC 1 cut(s) 198
BccI CCATC 4 cut(s) 137, 167, 212, 323
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 251, 285
BciT130I CCWGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 2 cut(s) 51, 440
BfmI CTRYAG 1 cut(s) 250
BglI GCCNNNNNGGC 1 cut(s) 195
BglII AGATCT 1 cut(s) 294
BisI GCNGC 1 cut(s) 187
BlsI GCNGC 1 cut(s) 188
BmcAI AGTACT 1 cut(s) 476
Bme1390I CCNGG 1 cut(s) 458
BmiI GGNNCC 2 cut(s) 157, 337
BmrFI CCNGG 1 cut(s) 458
BmsI GCATC 3 cut(s) 67, 259, 431
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
BsaI GGTCTC 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 1 cut(s) 458
BseGI GGATG 3 cut(s) 39, 90, 422
BseMII CTCAG 1 cut(s) 15
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 1 cut(s) 198
BshFI GGCC 2 cut(s) 65, 461
BsiHKAI GWGCWC 2 cut(s) 353, 399
BsmAI GTCTC 1 cut(s) 299
BsnI GGCC 2 cut(s) 65, 461
Bso31I GGTCTC 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 353, 399
Bsp143I GATC 5 cut(s) 168, 258, 294, 335, 384
BspANI GGCC 2 cut(s) 65, 461
BspCNI CTCAG 1 cut(s) 16
BspLI GGNNCC 2 cut(s) 157, 337
BspPI GGATC 2 cut(s) 330, 343
BspQI GCTCTTC 1 cut(s) 387
BspTNI GGTCTC 1 cut(s) 299
BsrI ACTGG 1 cut(s) 190
BssMI GATC 5 cut(s) 168, 258, 294, 335, 384
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 254, 479
Bst6I CTCTTC 3 cut(s) 387, 437, 441
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 3 cut(s) 39, 90, 422
BstKTI GATC 5 cut(s) 171, 261, 297, 338, 387
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 5 cut(s) 168, 258, 294, 335, 384
BstMWI GCNNNNNNNGC 2 cut(s) 110, 195
BstNI CCWGG 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 456
BstSFI CTRYAG 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 2 cut(s) 294, 335
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 2 cut(s) 294, 335
BsuRI GGCC 2 cut(s) 65, 461
BtsCI GGATG 3 cut(s) 39, 90, 422
Cac8I GCNNGC 1 cut(s) 409
Csp6I GTAC 2 cut(s) 451, 475
CviJI RGCY 9 cut(s) 50, 65, 125, 189, 198, 320, 407, 439, 461
CviKI_1 RGCY 9 cut(s) 50, 65, 125, 189, 198, 320, 407, 439, 461
CviQI GTAC 2 cut(s) 451, 475
DdeI CTNAG 1 cut(s) 24
DpnI GATC 5 cut(s) 170, 260, 296, 337, 386
DpnII GATC 5 cut(s) 168, 258, 294, 335, 384
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 387, 437, 441
EarI CTCTTC 3 cut(s) 387, 437, 441
Eco147I AGGCCT 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 456
FaiI YATR 1 cut(s) 18
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 1 cut(s) 187
FokI GGATG 3 cut(s) 26, 77, 409
Fsp4HI GCNGC 1 cut(s) 187
FspBI CTAG 2 cut(s) 51, 440
GluI GCNGC 1 cut(s) 187
HaeIII GGCC 2 cut(s) 65, 461
HphI GGTGA 4 cut(s) 22, 163, 191, 386
Hpy166II GTNNAC 2 cut(s) 30, 244
Hpy188I TCNGA 2 cut(s) 280, 328
Hpy188III TCNNGA 2 cut(s) 163, 346
Hpy8I GTNNAC 2 cut(s) 30, 244
HpyAV CCTTC 5 cut(s) 131, 176, 184, 257, 412
HpyCH4III ACNGT 2 cut(s) 254, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 195
HpyF3I CTNAG 1 cut(s) 24
Kzo9I GATC 5 cut(s) 168, 258, 294, 335, 384
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 6 cut(s) 60, 203, 287, 393, 443, 470
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 3 cut(s) 67, 259, 431
MaeI CTAG 2 cut(s) 51, 440
MaeIII GTNAC 1 cut(s) 202
MalI GATC 5 cut(s) 170, 260, 296, 337, 386
MboI GATC 5 cut(s) 168, 258, 294, 335, 384
MboII GAAGA 6 cut(s) 178, 304, 315, 404, 424, 458
MflI RGATCY 2 cut(s) 294, 335
MhlI GDGCHC 2 cut(s) 353, 399
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 2 cut(s) 129, 311
MluNI TGGCCA 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 5 cut(s) 43, 140, 326, 440, 464
Mox20I TGGCCA 1 cut(s) 461
MscI TGGCCA 1 cut(s) 461
MseI TTAA 2 cut(s) 402, 481
Msp20I TGGCCA 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 458
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 2 cut(s) 110, 195
NdeII GATC 5 cut(s) 168, 258, 294, 335, 384
NlaIV GGNNCC 2 cut(s) 157, 337
PceI AGGCCT 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 387
PkrI GCNGC 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 157, 337
PsuI RGATCY 2 cut(s) 294, 335
RsaI GTAC 2 cut(s) 452, 476
RsaNI GTAC 2 cut(s) 451, 475
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 402, 481
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 5 cut(s) 168, 258, 294, 335, 384
ScaI AGTACT 1 cut(s) 476
ScrFI CCNGG 1 cut(s) 458
SduI GDGCHC 2 cut(s) 353, 399
SetI ASST 7 cut(s) 35, 52, 176, 376, 432, 441, 456
SfaNI GCATC 3 cut(s) 67, 259, 431
SfcI CTRYAG 1 cut(s) 250
Sse9I AATT 2 cut(s) 129, 311
SseBI AGGCCT 1 cut(s) 65
SspMI CTAG 2 cut(s) 51, 440
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 456
TaaI ACNGT 2 cut(s) 254, 479
TaqI TCGA 1 cut(s) 261
TasI AATT 2 cut(s) 129, 311
TatI WGTACW 1 cut(s) 474
Tru1I TTAA 2 cut(s) 402, 481
Tru9I TTAA 2 cut(s) 402, 481
TseI GCWGC 1 cut(s) 186
TspDTI ATGAA 1 cut(s) 33
TspGWI ACGGA 2 cut(s) 25, 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 2 cut(s) 51, 440
ZrmI AGTACT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.