Rh4CG361700

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012188
Physical Location & Seq
Reverse (-)
61839090 .. 61840992
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG361700.1

Sequence Viewer

Length: 996 bp
ATGGTCGGAAAGAGGAAGGAAACGACGACTTCCTTGGAGGACTTGATAGAGAATGCAGGAGGTTGCGCTGTCGTGGACGGAGGGTTTGCCACACAGCTCGAGAAACACGGCGCTGCTATCAACGACACTCTCTGGAGCGCCGTTTGCCTCATCAAGGACTCCGACCTCATCAAAAGGGTCCACTTGGAATACTTGGAAGCCGGCGCCGATGTTCTGGTCACTTCATCTTACCAGGCAACAATTCCGGGATTTTTGTCCAAGGGACTGTCCATCGAAGAAGGGGAGTCATTGCTGAAGAAGAGTGTGACGTTAGCTGTCGAAGCTCGTAATAGTTTCTGGGAGGCAGTGAAAGTAAGTCCCCGGCATCATCGCTATAACAGGGCTTTGGTTGCGGCCTCTATCGGAAGCTATGGGGCTTATCTTGCTGATGGTTCTGAATACAGTGGGGATTATGGACCAAATGTGGATATGGATAAGCTGAAGGATTTCCACCGCCGCAGATTGCAAGTTCTTGTGGAAGCAGGTCCGGATTTACTTGCATTTGAGACCATTCCTAATAAGCTTGAAGCTCAGGCCTGCGTTGAGCTGCTGGAAGAAGAAAACATTCATATGCCATCTTGGATATGTTTCAGCTCGGTAGATGGTGAGAATGCGCCATCTGGAGAGAACTTCAAGGAATGCCTTGACATAATAAATAGCAGTAACAAAGTAAACGCAGTGGGGATAAATTGTGCACCTCCTCAGTTGATTGAAAGTCTCATCTGCAAATTGAAGCAGTTAACCAGTAAGGCAATTGTTGTGTACCCTAATAGTGGTGAGATATGGGATGGCAAAGCAAAGAGATGGCTGCCTTCCAAGTGCTTCGATGATGACGAGTTCGAATGCTTTGCGACAAGATGGCGCGATTCAGGAGCTAAGCTCATTGGAGGCTGTTGCCGGACAACACCATCAACCATTCAAGCAATTTCTAAGGTTCTAAAGGGAAAATCCCAATGA

Protein Analysis

331

Amino Acids

36.19

Weight (kDa)

5.42

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 325 6.5e-77 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 512
AccB1I GGYRCC 1 cut(s) 203
AccII CGCG 1 cut(s) 903
AccIII TCCGGA 1 cut(s) 526
AciI CCGC 3 cut(s) 392, 493, 496
AcuI CTGAAG 2 cut(s) 314, 500
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 2 cut(s) 154, 812
AgsI TTSAA 5 cut(s) 566, 673, 752, 772, 959
AjnI CCWGG 1 cut(s) 231
Alw21I GWGCWC 1 cut(s) 736
Alw26I GTCTC 2 cut(s) 539, 761
Alw44I GTGCAC 1 cut(s) 732
Ama87I CYCGRG 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 526
AoxI GGCC 2 cut(s) 393, 573
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 3 cut(s) 113, 586, 847
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 2 cut(s) 485, 603
AspLEI GCGC 6 cut(s) 68, 113, 140, 206, 655, 903
AspS9I GGNCC 3 cut(s) 178, 455, 524
AsuC2I CCSGG 2 cut(s) 246, 361
AsuHPI GGTGA 2 cut(s) 656, 827
AsuII TTCGAA 1 cut(s) 879
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 3 cut(s) 178, 455, 524
BaeGI GKGCMC 1 cut(s) 736
BanI GGYRCC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 736
BbvI GCAGC 3 cut(s) 100, 573, 834
BccI CCATC 9 cut(s) 278, 422, 622, 635, 664, 821, 837, 891, 955
BceAI ACGGC 2 cut(s) 124, 125
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BciT130I CCWGG 1 cut(s) 233
BcnI CCSGG 2 cut(s) 246, 361
BcoDI GTCTC 2 cut(s) 539, 761
BfoI RGCGCY 3 cut(s) 114, 141, 207
BfuAI ACCTGC 1 cut(s) 512
BisI GCNGC 5 cut(s) 114, 393, 496, 587, 848
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 5 cut(s) 115, 394, 497, 588, 849
Bme1390I CCNGG 3 cut(s) 233, 246, 361
Bme18I GGWCC 3 cut(s) 178, 455, 524
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 178, 455, 524
BmiI GGNNCC 2 cut(s) 179, 205
BmrFI CCNGG 3 cut(s) 233, 246, 361
BmsI GCATC 1 cut(s) 373
BplI GAGNNNNNCTC 2 cut(s) 903, 935
BpmI CTGGAG 2 cut(s) 154, 681
Bpu10I CCTNAGC 1 cut(s) 570
Bpu1102I GCTNAGC 1 cut(s) 915
Bpu14I TTCGAA 1 cut(s) 879
BpuMI CCSGG 2 cut(s) 246, 361
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 3 cut(s) 33, 258, 359
BsaWI WCCGGW 1 cut(s) 526
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 812
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 783
Bse3DI GCAATG 1 cut(s) 287
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 3 cut(s) 33, 258, 359
BseGI GGATG 1 cut(s) 832
BseLI CCNNNNNNNGG 2 cut(s) 154, 812
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 2 cut(s) 584, 755
BseNI ACTGG 1 cut(s) 783
BseRI GAGGAG 1 cut(s) 729
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 3 cut(s) 100, 573, 834
Bsh1236I CGCG 1 cut(s) 903
BshFI GGCC 2 cut(s) 395, 575
BshNI GGYRCC 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 736
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 5 cut(s) 201, 245, 361, 527, 937
BslFI GGGAC 2 cut(s) 276, 342
BslI CCNNNNNNNGG 2 cut(s) 154, 812
BsmAI GTCTC 2 cut(s) 539, 761
BsmFI GGGAC 2 cut(s) 276, 342
BsmI GAATGC 4 cut(s) 58, 655, 683, 887
BsnI GGCC 2 cut(s) 395, 575
Bso31I GGTCTC 1 cut(s) 539
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 879
Bsp1286I GDGCHC 1 cut(s) 736
Bsp13I TCCGGA 1 cut(s) 526
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 3 cut(s) 392, 493, 496
BspANI GGCC 2 cut(s) 395, 575
BspCNI CTCAG 2 cut(s) 583, 754
BspEI TCCGGA 1 cut(s) 526
BspFNI CGCG 1 cut(s) 903
BspLI GGNNCC 2 cut(s) 179, 205
BspMI ACCTGC 1 cut(s) 512
BspT104I TTCGAA 1 cut(s) 879
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 783
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 33, 258, 359
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 33, 258
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 267, 443
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 879
BstC8I GCNNGC 2 cut(s) 202, 577
BstDEI CTNAG 4 cut(s) 570, 741, 915, 969
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 1 cut(s) 832
BstFNI CGCG 1 cut(s) 903
BstH2I RGCGCY 3 cut(s) 114, 141, 207
BstHHI GCGC 6 cut(s) 68, 113, 140, 206, 655, 903
BstMAI GTCTC 2 cut(s) 539, 761
BstMWI GCNNNNNNNGC 4 cut(s) 144, 320, 389, 422
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 231, 244, 359
BstSLI GKGCMC 1 cut(s) 736
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 3 cut(s) 100, 573, 834
BsuRI GGCC 2 cut(s) 395, 575
BtgZI GCGATG 1 cut(s) 353
BtsCI GGATG 1 cut(s) 832
BtsI GCAGTG 2 cut(s) 351, 723
BtsIMutI CAGTG 3 cut(s) 351, 448, 723
BveI ACCTGC 1 cut(s) 512
Cac8I GCNNGC 2 cut(s) 202, 577
CfoI GCGC 6 cut(s) 68, 113, 140, 206, 655, 903
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 178, 455, 524
Csp6I GTAC 1 cut(s) 802
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 4 cut(s) 570, 741, 915, 969
DinI GGCGCC 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 33, 258
Eco147I AGGCCT 1 cut(s) 575
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 3 cut(s) 178, 455, 524
Eco57I CTGAAG 2 cut(s) 314, 500
Eco88I CYCGRG 1 cut(s) 98
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 231
EcoT14I CCWWGG 2 cut(s) 33, 258
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 33, 258
FaiI YATR 9 cut(s) 375, 411, 453, 470, 609, 611, 625, 689, 823
FaqI GGGAC 2 cut(s) 276, 342
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 5 cut(s) 114, 393, 496, 587, 848
FokI GGATG 1 cut(s) 839
Fsp4HI GCNGC 5 cut(s) 114, 393, 496, 587, 848
GlaI GCGC 6 cut(s) 67, 112, 139, 205, 654, 902
GluI GCNGC 5 cut(s) 114, 393, 496, 587, 848
GsuI CTGGAG 2 cut(s) 154, 681
HaeII RGCGCY 3 cut(s) 114, 141, 207
HaeIII GGCC 2 cut(s) 395, 575
HapII CCGG 5 cut(s) 201, 245, 361, 527, 937
HhaI GCGC 6 cut(s) 68, 113, 140, 206, 655, 903
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 6 cut(s) 66, 111, 138, 204, 653, 901
HinP1I GCGC 6 cut(s) 66, 111, 138, 204, 653, 901
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 3 cut(s) 158, 284, 905
HpaI GTTAAC 1 cut(s) 780
HpaII CCGG 5 cut(s) 201, 245, 361, 527, 937
HphI GGTGA 2 cut(s) 656, 827
Hpy166II GTNNAC 6 cut(s) 76, 181, 712, 734, 780, 802
Hpy188I TCNGA 4 cut(s) 8, 163, 404, 436
Hpy188III TCNNGA 5 cut(s) 100, 133, 527, 660, 909
Hpy8I GTNNAC 6 cut(s) 76, 181, 712, 734, 780, 802
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 4 cut(s) 10, 272, 475, 861
HpyCH4III ACNGT 2 cut(s) 267, 443
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 5 cut(s) 56, 505, 539, 734, 765
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 320, 389, 422
HpyF3I CTNAG 4 cut(s) 570, 741, 915, 969
HpySE526I ACGT 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 6 cut(s) 66, 111, 138, 204, 653, 901
KasI GGCGCC 1 cut(s) 203
Kpn2I TCCGGA 1 cut(s) 526
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
KspAI GTTAAC 1 cut(s) 780
LmnI GCTCC 2 cut(s) 135, 911
Lsp1109I GCAGC 3 cut(s) 100, 573, 834
LweI GCATC 1 cut(s) 373
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 3 cut(s) 217, 304, 701
MboII GAAGA 5 cut(s) 287, 307, 310, 605, 608
MfeI CAATTG 1 cut(s) 792
MhlI GDGCHC 1 cut(s) 736
MluCI AATT 5 cut(s) 240, 727, 767, 792, 963
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 152, 293
MmeI TCCRAC 1 cut(s) 186
MroI TCCGGA 1 cut(s) 526
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 485, 603
MseI TTAA 1 cut(s) 779
MslI CAYNNNNRTG 1 cut(s) 608
MspI CCGG 5 cut(s) 201, 245, 361, 527, 937
MspR9I CCNGG 3 cut(s) 233, 246, 361
MunI CAATTG 1 cut(s) 792
Mva1269I GAATGC 4 cut(s) 58, 655, 683, 887
MvaI CCWGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 4 cut(s) 144, 320, 389, 422
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 2 cut(s) 246, 361
NdeI CATATG 1 cut(s) 609
NgoMIV GCCGGC 1 cut(s) 200
NlaIV GGNNCC 2 cut(s) 179, 205
NmuCI GTSAC 2 cut(s) 217, 304
NspV TTCGAA 1 cut(s) 879
PaeR7I CTCGAG 1 cut(s) 98
PceI AGGCCT 1 cut(s) 575
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PctI GAATGC 4 cut(s) 58, 655, 683, 887
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 2 cut(s) 485, 603
PfeI GAWTC 1 cut(s) 905
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 5 cut(s) 115, 394, 497, 588, 849
PleI GAGTC 2 cut(s) 152, 292
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 2 cut(s) 152, 292
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 179, 205
PspPI GGNCC 3 cut(s) 178, 455, 524
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
RseI CAYNNNNRTG 1 cut(s) 608
SaqAI TTAA 1 cut(s) 779
SatI GCNGC 5 cut(s) 114, 393, 496, 587, 848
Sau96I GGNCC 3 cut(s) 178, 455, 524
SchI GAGTC 2 cut(s) 152, 293
ScrFI CCNGG 3 cut(s) 233, 246, 361
SduI GDGCHC 1 cut(s) 736
SfaNI GCATC 1 cut(s) 373
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 98
SfuI TTCGAA 1 cut(s) 879
SinI GGWCC 3 cut(s) 178, 455, 524
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 5 cut(s) 240, 727, 767, 792, 963
SseBI AGGCCT 1 cut(s) 575
SsiI CCGC 3 cut(s) 392, 493, 496
SspDI GGCGCC 1 cut(s) 203
StuI AGGCCT 1 cut(s) 575
StyD4I CCNGG 3 cut(s) 231, 244, 359
StyI CCWWGG 2 cut(s) 33, 258
TaaI ACNGT 2 cut(s) 267, 443
TaiI ACGT 1 cut(s) 311
TaqI TCGA 5 cut(s) 99, 273, 318, 864, 879
TasI AATT 5 cut(s) 240, 727, 767, 792, 963
TauI GCSGC 2 cut(s) 395, 498
TfiI GAWTC 1 cut(s) 905
Tru1I TTAA 1 cut(s) 779
Tru9I TTAA 1 cut(s) 779
TscAI CASTG 3 cut(s) 351, 448, 723
TseFI GTSAC 2 cut(s) 217, 304
TseI GCWGC 3 cut(s) 113, 586, 847
Tsp45I GTSAC 2 cut(s) 217, 304
TspDTI ATGAA 2 cut(s) 213, 596
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 3 cut(s) 351, 448, 723
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 3 cut(s) 178, 455, 524
XagI CCTNNNNNAGG 1 cut(s) 152
XhoI CTCGAG 1 cut(s) 98
XmnI GAANNNNTTC 2 cut(s) 485, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.