Rw4G029560

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012188
Physical Location & Seq
Reverse (-)
56220828 .. 56223267
2440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029560.1

Sequence Viewer

Length: 798 bp
ATGGTCGTAAAGAAGAAGGAAACGACGACTTCCTTGGAGGACTTGATAGAGAATGCAGGAGGTTGCGCTGTCGTGGACGGAGGGTTTGCCACACAGCTCGAGAAACACGGCGCTGCTATCAACGACACTCTCTGGAGCGCCGTTTGCCTCATCAAGGACCCCGACCTCATCAAAAGGGTCCACTTGGAATACTTGGAAGCCGGCGCCGATGTTCTGGTCACTTCATCTTACCAGGCAACAATTCCGGGATTTTTGTCCAAGGGACTGTCCATTGAAGAAGGGGAGTCATTGCTGAAGAAGAGTGTGACGTTAGCTGTCGAAGCTCGTAATAGTTTCTGGGAGGCAGTGAAAGTAAGTCCCCGGCATCATCGCTATAACAGGGCTTTGGTTGCGGCCTCTATCGGAAGCTATGGGGCTTATCTTGCTGATGGTTCTGAATACAGTGGGGATTATGGACCAAATGTGGATATGGATAAGCTGAAGGATTTCCACCGCCGCAGATTGCAAGTTCTTGTGGAAGCAGGTCCGGATTTACTTGCATTTGAGACCATTCCTAATAAGCTTGAAGCTCAGGCCTGCGTTGAGCTGCTCGAAGAAGAAAACATTCATATGCCATCTTGGATATGTTTCAGCTCTGTAGATGGTGAGAATGCACCATCTGGAGAGAACTTCAAGGAATGCCTTGACATAATAAATAGCAGTAACAAAGTAAACGCAGTGGGGATAAATTGTGCACCTCCTCAGTTGATTGAAAGTCTCATCTGCAAATTGAAGCAGGTAATTTCTTGGTTTACCTAA

Protein Analysis

265

Amino Acids

28.97

Weight (kDa)

4.99

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 24 - 259 5.9e-59 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 512, 766
AccB1I GGYRCC 1 cut(s) 203
AccIII TCCGGA 1 cut(s) 526
AciI CCGC 3 cut(s) 392, 493, 496
AcuI CTGAAG 2 cut(s) 314, 500
AcyI GRCGYC 1 cut(s) 204
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 5 cut(s) 275, 566, 673, 752, 772
AjnI CCWGG 1 cut(s) 231
AluBI AGCT 9 cut(s) 97, 314, 323, 408, 478, 562, 569, 586, 633
AluI AGCT 9 cut(s) 97, 314, 323, 408, 478, 562, 569, 586, 633
Alw21I GWGCWC 1 cut(s) 736
Alw26I GTCTC 2 cut(s) 539, 761
Alw44I GTGCAC 1 cut(s) 732
Ama87I CYCGRG 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 526
AoxI GGCC 2 cut(s) 393, 573
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 2 cut(s) 113, 586
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 2 cut(s) 485, 603
AspLEI GCGC 4 cut(s) 68, 113, 140, 206
AspS9I GGNCC 4 cut(s) 157, 178, 455, 524
AsuC2I CCSGG 2 cut(s) 246, 361
AsuHPI GGTGA 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 4 cut(s) 157, 178, 455, 524
BaeGI GKGCMC 1 cut(s) 736
BanI GGYRCC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 736
BbvI GCAGC 2 cut(s) 100, 573
BccI CCATC 4 cut(s) 422, 622, 635, 664
BceAI ACGGC 2 cut(s) 124, 125
BciT130I CCWGG 1 cut(s) 233
BcnI CCSGG 2 cut(s) 246, 361
BcoDI GTCTC 2 cut(s) 539, 761
BfmI CTRYAG 1 cut(s) 636
BfoI RGCGCY 3 cut(s) 114, 141, 207
BfuAI ACCTGC 2 cut(s) 512, 766
BisI GCNGC 4 cut(s) 114, 393, 496, 587
BlsI GCNGC 4 cut(s) 115, 394, 497, 588
Bme1390I CCNGG 3 cut(s) 233, 246, 361
Bme18I GGWCC 4 cut(s) 157, 178, 455, 524
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 4 cut(s) 157, 178, 455, 524
BmiI GGNNCC 3 cut(s) 159, 179, 205
BmrFI CCNGG 3 cut(s) 233, 246, 361
BmsI GCATC 1 cut(s) 373
BpmI CTGGAG 2 cut(s) 154, 681
Bpu10I CCTNAGC 1 cut(s) 570
BpuMI CCSGG 2 cut(s) 246, 361
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 3 cut(s) 33, 258, 359
BsaWI WCCGGW 1 cut(s) 526
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse118I RCCGGY 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 287
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 3 cut(s) 33, 258, 359
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 2 cut(s) 584, 755
BseRI GAGGAG 1 cut(s) 729
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 2 cut(s) 100, 573
BshFI GGCC 2 cut(s) 395, 575
BshNI GGYRCC 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 736
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 4 cut(s) 201, 245, 361, 527
BslFI GGGAC 2 cut(s) 276, 342
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 539, 761
BsmFI GGGAC 2 cut(s) 276, 342
BsmI GAATGC 3 cut(s) 58, 655, 683
BsnI GGCC 2 cut(s) 395, 575
Bso31I GGTCTC 1 cut(s) 539
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 736
Bsp13I TCCGGA 1 cut(s) 526
BspACI CCGC 3 cut(s) 392, 493, 496
BspANI GGCC 2 cut(s) 395, 575
BspCNI CTCAG 2 cut(s) 583, 754
BspEI TCCGGA 1 cut(s) 526
BspLI GGNNCC 3 cut(s) 159, 179, 205
BspMI ACCTGC 2 cut(s) 512, 766
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 33, 258, 359
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 33, 258
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 267, 443
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 202, 577
BstDEI CTNAG 2 cut(s) 570, 741
BstENI CCTNNNNNAGG 1 cut(s) 152
BstH2I RGCGCY 3 cut(s) 114, 141, 207
BstHHI GCGC 4 cut(s) 68, 113, 140, 206
BstMAI GTCTC 2 cut(s) 539, 761
BstMWI GCNNNNNNNGC 4 cut(s) 144, 320, 389, 422
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 231, 244, 359
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 1 cut(s) 736
BstV1I GCAGC 2 cut(s) 100, 573
BsuRI GGCC 2 cut(s) 395, 575
BtgZI GCGATG 1 cut(s) 353
BtsI GCAGTG 2 cut(s) 351, 723
BtsIMutI CAGTG 3 cut(s) 351, 448, 723
BveI ACCTGC 2 cut(s) 512, 766
Cac8I GCNNGC 2 cut(s) 202, 577
CfoI GCGC 4 cut(s) 68, 113, 140, 206
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 4 cut(s) 157, 178, 455, 524
DdeI CTNAG 2 cut(s) 570, 741
DinI GGCGCC 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 33, 258
Eco147I AGGCCT 1 cut(s) 575
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 4 cut(s) 157, 178, 455, 524
Eco57I CTGAAG 2 cut(s) 314, 500
Eco88I CYCGRG 1 cut(s) 98
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 231
EcoT14I CCWWGG 2 cut(s) 33, 258
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 33, 258
FaiI YATR 8 cut(s) 375, 411, 453, 470, 609, 611, 625, 689
FaqI GGGAC 2 cut(s) 276, 342
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 4 cut(s) 114, 393, 496, 587
Fsp4HI GCNGC 4 cut(s) 114, 393, 496, 587
GlaI GCGC 4 cut(s) 67, 112, 139, 205
GluI GCNGC 4 cut(s) 114, 393, 496, 587
GsuI CTGGAG 2 cut(s) 154, 681
HaeII RGCGCY 3 cut(s) 114, 141, 207
HaeIII GGCC 2 cut(s) 395, 575
HapII CCGG 4 cut(s) 201, 245, 361, 527
HhaI GCGC 4 cut(s) 68, 113, 140, 206
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 4 cut(s) 66, 111, 138, 204
HinP1I GCGC 4 cut(s) 66, 111, 138, 204
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 1 cut(s) 284
HpaII CCGG 4 cut(s) 201, 245, 361, 527
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 5 cut(s) 76, 181, 712, 734, 792
Hpy188I TCNGA 2 cut(s) 404, 436
Hpy188III TCNNGA 4 cut(s) 100, 133, 527, 660
Hpy8I GTNNAC 5 cut(s) 76, 181, 712, 734, 792
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 10, 272, 475
HpyCH4III ACNGT 2 cut(s) 267, 443
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 6 cut(s) 56, 505, 539, 653, 734, 765
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 320, 389, 422
HpyF3I CTNAG 2 cut(s) 570, 741
HpySE526I ACGT 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 4 cut(s) 66, 111, 138, 204
KasI GGCGCC 1 cut(s) 203
Kpn2I TCCGGA 1 cut(s) 526
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
LmnI GCTCC 1 cut(s) 135
Lsp1109I GCAGC 2 cut(s) 100, 573
LweI GCATC 1 cut(s) 373
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 3 cut(s) 217, 304, 701
MboII GAAGA 6 cut(s) 25, 287, 307, 310, 605, 608
MhlI GDGCHC 1 cut(s) 736
MluCI AATT 4 cut(s) 240, 727, 767, 780
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 1 cut(s) 293
MnlI CCTC 9 cut(s) 31, 53, 74, 158, 176, 334, 406, 747, 750
MroI TCCGGA 1 cut(s) 526
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 485, 603
MslI CAYNNNNRTG 1 cut(s) 608
MspI CCGG 4 cut(s) 201, 245, 361, 527
MspR9I CCNGG 3 cut(s) 233, 246, 361
Mva1269I GAATGC 3 cut(s) 58, 655, 683
MvaI CCWGG 1 cut(s) 233
MwoI GCNNNNNNNGC 4 cut(s) 144, 320, 389, 422
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 2 cut(s) 246, 361
NdeI CATATG 1 cut(s) 609
NgoMIV GCCGGC 1 cut(s) 200
NlaIV GGNNCC 3 cut(s) 159, 179, 205
NmuCI GTSAC 2 cut(s) 217, 304
PaeR7I CTCGAG 1 cut(s) 98
PceI AGGCCT 1 cut(s) 575
PctI GAATGC 3 cut(s) 58, 655, 683
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 2 cut(s) 485, 603
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 4 cut(s) 115, 394, 497, 588
PleI GAGTC 1 cut(s) 292
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 292
PpuMI RGGWCCY 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 3 cut(s) 159, 179, 205
PspPI GGNCC 4 cut(s) 157, 178, 455, 524
PspPPI RGGWCCY 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 608
SatI GCNGC 4 cut(s) 114, 393, 496, 587
Sau96I GGNCC 4 cut(s) 157, 178, 455, 524
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 3 cut(s) 233, 246, 361
SduI GDGCHC 1 cut(s) 736
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 1 cut(s) 636
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 4 cut(s) 157, 178, 455, 524
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 4 cut(s) 240, 727, 767, 780
SseBI AGGCCT 1 cut(s) 575
SsiI CCGC 3 cut(s) 392, 493, 496
SspDI GGCGCC 1 cut(s) 203
StuI AGGCCT 1 cut(s) 575
StyD4I CCNGG 3 cut(s) 231, 244, 359
StyI CCWWGG 2 cut(s) 33, 258
TaaI ACNGT 2 cut(s) 267, 443
TaiI ACGT 1 cut(s) 311
TaqI TCGA 3 cut(s) 99, 318, 591
TasI AATT 4 cut(s) 240, 727, 767, 780
TauI GCSGC 2 cut(s) 395, 498
TscAI CASTG 3 cut(s) 351, 448, 723
TseFI GTSAC 2 cut(s) 217, 304
TseI GCWGC 2 cut(s) 113, 586
Tsp45I GTSAC 2 cut(s) 217, 304
TspDTI ATGAA 2 cut(s) 213, 596
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 3 cut(s) 351, 448, 723
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 4 cut(s) 157, 178, 455, 524
XagI CCTNNNNNAGG 1 cut(s) 152
XhoI CTCGAG 1 cut(s) 98
XmnI GAANNNNTTC 2 cut(s) 485, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.