RLG00000006608

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012188
Physical Location & Seq
Forward (+)
8979550 .. 8981629
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006608

Sequence Viewer

Length: 1158 bp
ATGTCAAACGGACAATGTGAAAAGAACGAACCTGTATGCCATTTCGTCCATATTCTTTTCTCTCCTAACACCCCCAACAACCACCTCCTCTCAGTCTTCGCTTTGCTTTGCTTTAAAACCCTCCGCAATTCGGTCTCTCTTCGCTTGCAGACAAACGCAGATATGGTCGGAAAGAAGAAGGAAATGACGACTTCCTTGGAGGACTTGATAGAGAATGCAGGAGGTTGCGCTGTCGTGGACGGAGGGTTTGCCACACAGCTCGAGAAACACGGCGCTGCTATCAACGACACTCTCTGGAGCGCCGTTTGCCTCATCAAGGACCCCGACCTCATCAAAAGGGTCCACTTAGAATACTTGGAAGCCGGCGCCGATGTTCTGGTCACTTCATCTTACCAGGCAACAATTCCGGGATTTTTGTCCAAGGGACTGTCCATCGAAGAAGGGGAGTCATTGCTGAAGAAGAGTGTGACGTTAGCTGTCGAAGCTCGTAATAGTTTCTGGGAGGCGGTGAAAGTAAGTCCCCGGCATCATCGCTATAACAGGGCTTTGGTTGCGGCCTCTATTGGAAGCTATGGGGCTTATCTTGCTGATGGTTCTGAATACAGTGGGGATTATGGACCAAACGTGGATATGGATAAGCTGAAGGATTTCCACCGCCGCAGATTGCAAGTTCTTGTGGAAGCAGGTCCGGATTTACTTGCATTTGAGACCATTCCTAATAAGCTTGAAGCTCAGGCCTGCGTTGAGCTGCTCGAAGAAGAAAACATTCATATGCCATCTTGGATATGTTTCAGCTCTGTAGATGGTGAGAATGCGCCATCTGGAGAGAACTTCAAGGAATGCGTTGACATAATAAATAGCAGTAACAAAGTAAACGCAGTGGGGATAAATTGTGCACCTCCTCAGTTGATTGAAAGTCTCATCTGCAAATTGAAGCAGTTAACCAGTAAGGCAATTGTTGTGTACCCTAATAGTGGTGAGATATGGGATGGCAAAGCAAAGAGATGGCTGCCTTCCAAGTGCTTCGATGATGACGAGTTCGAATGCTTTGCAACAAGATGGCGCGATTCAGGAGCTAAGCTCATTGGAGGCTGTTGCCGGACAACACCATCAACCATTCAAGCAATTTCTAAGGTTCTAAAGGGGAAATCCCAATGA

Protein Analysis

386

Amino Acids

42.27

Weight (kDa)

5.79

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 78 - 379 6.8e-78 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 365
AccII CGCG 1 cut(s) 1065
AccIII TCCGGA 1 cut(s) 688
AciI CCGC 5 cut(s) 124, 506, 554, 655, 658
AcuI CTGAAG 2 cut(s) 476, 662
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 1 cut(s) 965
AfiI CCNNNNNNNGG 3 cut(s) 130, 316, 974
AgsI TTSAA 5 cut(s) 728, 835, 914, 934, 1121
AjnI CCWGG 1 cut(s) 393
Alw21I GWGCWC 1 cut(s) 898
Alw26I GTCTC 3 cut(s) 139, 701, 923
Alw44I GTGCAC 1 cut(s) 894
Ama87I CYCGRG 1 cut(s) 260
Aor13HI TCCGGA 1 cut(s) 688
AoxI GGCC 2 cut(s) 555, 735
ApaLI GTGCAC 1 cut(s) 894
ApeKI GCWGC 3 cut(s) 275, 748, 1009
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
Asp700I GAANNNNTTC 2 cut(s) 647, 765
AspLEI GCGC 6 cut(s) 230, 275, 302, 368, 817, 1065
AspS9I GGNCC 4 cut(s) 319, 340, 617, 686
AsuC2I CCSGG 2 cut(s) 408, 523
AsuHPI GGTGA 3 cut(s) 520, 818, 989
AsuII TTCGAA 1 cut(s) 1041
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 4 cut(s) 319, 340, 617, 686
BaeGI GKGCMC 1 cut(s) 898
BanI GGYRCC 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 898
BbvI GCAGC 3 cut(s) 262, 735, 996
BccI CCATC 9 cut(s) 440, 584, 784, 797, 826, 983, 999, 1053, 1117
BceAI ACGGC 2 cut(s) 286, 287
BcgI CGANNNNNNTGC 2 cut(s) 1031, 1065
BciT130I CCWGG 1 cut(s) 395
BcnI CCSGG 2 cut(s) 408, 523
BcoDI GTCTC 3 cut(s) 139, 701, 923
BfmI CTRYAG 1 cut(s) 798
BfoI RGCGCY 3 cut(s) 276, 303, 369
BfuAI ACCTGC 1 cut(s) 674
BisI GCNGC 5 cut(s) 276, 555, 658, 749, 1010
BlpI GCTNAGC 1 cut(s) 1077
BlsI GCNGC 5 cut(s) 277, 556, 659, 750, 1011
Bme1390I CCNGG 3 cut(s) 395, 408, 523
Bme18I GGWCC 4 cut(s) 319, 340, 617, 686
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 4 cut(s) 319, 340, 617, 686
BmiI GGNNCC 3 cut(s) 321, 341, 367
BmrFI CCNGG 3 cut(s) 395, 408, 523
BmsI GCATC 1 cut(s) 535
BpiI GAAGAC 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 1065, 1097
BpmI CTGGAG 2 cut(s) 316, 843
Bpu10I CCTNAGC 1 cut(s) 732
Bpu1102I GCTNAGC 1 cut(s) 1077
Bpu14I TTCGAA 1 cut(s) 1041
BpuMI CCSGG 2 cut(s) 408, 523
BsaHI GRCGYC 1 cut(s) 366
BsaI GGTCTC 2 cut(s) 139, 701
BsaJI CCNNGG 3 cut(s) 195, 420, 521
BsaWI WCCGGW 1 cut(s) 688
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 316, 974
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 1 cut(s) 945
Bse3DI GCAATG 1 cut(s) 449
BseAI TCCGGA 1 cut(s) 688
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 3 cut(s) 195, 420, 521
BseGI GGATG 1 cut(s) 994
BseLI CCNNNNNNNGG 3 cut(s) 130, 316, 974
BseMI GCAATG 1 cut(s) 449
BseMII CTCAG 3 cut(s) 105, 746, 917
BseNI ACTGG 1 cut(s) 945
BseRI GAGGAG 2 cut(s) 77, 891
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 3 cut(s) 262, 735, 996
Bsh1236I CGCG 1 cut(s) 1065
BshFI GGCC 2 cut(s) 557, 737
BshNI GGYRCC 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 898
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 5 cut(s) 363, 407, 523, 689, 1099
BslFI GGGAC 2 cut(s) 438, 504
BslI CCNNNNNNNGG 3 cut(s) 130, 316, 974
BsmAI GTCTC 3 cut(s) 139, 701, 923
BsmFI GGGAC 2 cut(s) 438, 504
BsmI GAATGC 4 cut(s) 220, 817, 845, 1049
BsnI GGCC 2 cut(s) 557, 737
Bso31I GGTCTC 2 cut(s) 139, 701
BsoBI CYCGRG 1 cut(s) 260
Bsp119I TTCGAA 1 cut(s) 1041
Bsp1286I GDGCHC 1 cut(s) 898
Bsp13I TCCGGA 1 cut(s) 688
Bsp1720I GCTNAGC 1 cut(s) 1077
BspACI CCGC 5 cut(s) 124, 506, 554, 655, 658
BspANI GGCC 2 cut(s) 557, 737
BspCNI CTCAG 3 cut(s) 104, 745, 916
BspEI TCCGGA 1 cut(s) 688
BspFNI CGCG 1 cut(s) 1065
BspLI GGNNCC 3 cut(s) 321, 341, 367
BspMI ACCTGC 1 cut(s) 674
BspT104I TTCGAA 1 cut(s) 1041
BspT107I GGYRCC 1 cut(s) 365
BspTNI GGTCTC 2 cut(s) 139, 701
BsrDI GCAATG 1 cut(s) 449
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 1 cut(s) 945
BssAI RCCGGY 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 195, 420, 521
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 2 cut(s) 195, 420
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 2 cut(s) 429, 605
Bst6I CTCTTC 2 cut(s) 144, 455
BstACI GRCGYC 1 cut(s) 366
BstBI TTCGAA 1 cut(s) 1041
BstC8I GCNNGC 3 cut(s) 146, 364, 739
BstDEI CTNAG 6 cut(s) 91, 346, 732, 903, 1077, 1131
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 994
BstFNI CGCG 1 cut(s) 1065
BstH2I RGCGCY 3 cut(s) 276, 303, 369
BstHHI GCGC 6 cut(s) 230, 275, 302, 368, 817, 1065
BstMAI GTCTC 3 cut(s) 139, 701, 923
BstMWI GCNNNNNNNGC 4 cut(s) 306, 482, 551, 584
BstNI CCWGG 1 cut(s) 395
BstSCI CCNGG 3 cut(s) 393, 406, 521
BstSFI CTRYAG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 898
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 3 cut(s) 262, 735, 996
BstV2I GAAGAC 1 cut(s) 88
BsuRI GGCC 2 cut(s) 557, 737
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 1 cut(s) 994
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 2 cut(s) 610, 885
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 3 cut(s) 146, 364, 739
CfoI GCGC 6 cut(s) 230, 275, 302, 368, 817, 1065
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 4 cut(s) 319, 340, 617, 686
Csp6I GTAC 1 cut(s) 964
CviQI GTAC 1 cut(s) 964
DdeI CTNAG 6 cut(s) 91, 346, 732, 903, 1077, 1131
DinI GGCGCC 1 cut(s) 367
DraI TTTAAA 1 cut(s) 115
Eam1104I CTCTTC 2 cut(s) 144, 455
EarI CTCTTC 2 cut(s) 144, 455
Eco130I CCWWGG 2 cut(s) 195, 420
Eco147I AGGCCT 1 cut(s) 737
Eco31I GGTCTC 2 cut(s) 139, 701
Eco47I GGWCC 4 cut(s) 319, 340, 617, 686
Eco57I CTGAAG 2 cut(s) 476, 662
Eco88I CYCGRG 1 cut(s) 260
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 393
EcoT14I CCWWGG 2 cut(s) 195, 420
EgeI GGCGCC 1 cut(s) 367
EheI GGCGCC 1 cut(s) 367
ErhI CCWWGG 2 cut(s) 195, 420
FaqI GGGAC 2 cut(s) 438, 504
FauNDI CATATG 1 cut(s) 771
Fnu4HI GCNGC 5 cut(s) 276, 555, 658, 749, 1010
FokI GGATG 1 cut(s) 1001
Fsp4HI GCNGC 5 cut(s) 276, 555, 658, 749, 1010
GlaI GCGC 6 cut(s) 229, 274, 301, 367, 816, 1064
GluI GCNGC 5 cut(s) 276, 555, 658, 749, 1010
GsuI CTGGAG 2 cut(s) 316, 843
HaeII RGCGCY 3 cut(s) 276, 303, 369
HaeIII GGCC 2 cut(s) 557, 737
HapII CCGG 5 cut(s) 363, 407, 523, 689, 1099
HhaI GCGC 6 cut(s) 230, 275, 302, 368, 817, 1065
Hin1I GRCGYC 1 cut(s) 366
Hin6I GCGC 6 cut(s) 228, 273, 300, 366, 815, 1063
HinP1I GCGC 6 cut(s) 228, 273, 300, 366, 815, 1063
HincII GTYRAC 2 cut(s) 847, 942
HindII GTYRAC 2 cut(s) 847, 942
HindIII AAGCTT 1 cut(s) 722
HinfI GANTC 2 cut(s) 446, 1067
HpaI GTTAAC 1 cut(s) 942
HpaII CCGG 5 cut(s) 363, 407, 523, 689, 1099
HphI GGTGA 3 cut(s) 520, 818, 989
Hpy166II GTNNAC 7 cut(s) 238, 343, 847, 874, 896, 942, 964
Hpy188I TCNGA 2 cut(s) 170, 598
Hpy188III TCNNGA 5 cut(s) 262, 295, 689, 822, 1071
Hpy8I GTNNAC 7 cut(s) 238, 343, 847, 874, 896, 942, 964
HpyAV CCTTC 4 cut(s) 172, 434, 637, 1023
HpyCH4III ACNGT 2 cut(s) 429, 605
HpyCH4IV ACGT 2 cut(s) 470, 624
HpyCH4V TGCA 7 cut(s) 148, 218, 667, 701, 896, 927, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 306, 482, 551, 584
HpyF3I CTNAG 6 cut(s) 91, 346, 732, 903, 1077, 1131
HpySE526I ACGT 2 cut(s) 470, 624
Hsp92I GRCGYC 1 cut(s) 366
HspAI GCGC 6 cut(s) 228, 273, 300, 366, 815, 1063
KasI GGCGCC 1 cut(s) 365
Kpn2I TCCGGA 1 cut(s) 688
KroI GCCGGC 1 cut(s) 362
KroNI GCCGGC 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 942
LmnI GCTCC 2 cut(s) 297, 1073
Lsp1109I GCAGC 3 cut(s) 262, 735, 996
LweI GCATC 1 cut(s) 535
MaeII ACGT 2 cut(s) 470, 624
MaeIII GTNAC 3 cut(s) 379, 466, 863
MboII GAAGA 8 cut(s) 88, 131, 187, 449, 469, 472, 767, 770
MfeI CAATTG 1 cut(s) 954
MhlI GDGCHC 1 cut(s) 898
MluCI AATT 6 cut(s) 127, 402, 889, 929, 954, 1125
Mly113I GGCGCC 1 cut(s) 366
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 1 cut(s) 148
MroI TCCGGA 1 cut(s) 688
MroNI GCCGGC 1 cut(s) 362
MroXI GAANNNNTTC 2 cut(s) 647, 765
MseI TTAA 2 cut(s) 114, 941
MslI CAYNNNNRTG 1 cut(s) 770
MspI CCGG 5 cut(s) 363, 407, 523, 689, 1099
MspR9I CCNGG 3 cut(s) 395, 408, 523
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 4 cut(s) 220, 817, 845, 1049
MvaI CCWGG 1 cut(s) 395
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 4 cut(s) 306, 482, 551, 584
NaeI GCCGGC 1 cut(s) 364
NarI GGCGCC 1 cut(s) 366
NciI CCSGG 2 cut(s) 408, 523
NdeI CATATG 1 cut(s) 771
NgoMIV GCCGGC 1 cut(s) 362
NlaIV GGNNCC 3 cut(s) 321, 341, 367
NmuCI GTSAC 2 cut(s) 379, 466
NspV TTCGAA 1 cut(s) 1041
PaeR7I CTCGAG 1 cut(s) 260
PceI AGGCCT 1 cut(s) 737
PcsI WCGNNNNNNNCGW 1 cut(s) 1032
PctI GAATGC 4 cut(s) 220, 817, 845, 1049
PdiI GCCGGC 1 cut(s) 364
PdmI GAANNNNTTC 2 cut(s) 647, 765
PfeI GAWTC 1 cut(s) 1067
PfoI TCCNGGA 1 cut(s) 406
PkrI GCNGC 5 cut(s) 277, 556, 659, 750, 1011
PleI GAGTC 1 cut(s) 454
PluTI GGCGCC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 393
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 3 cut(s) 321, 341, 367
PspPI GGNCC 4 cut(s) 319, 340, 617, 686
PspPPI RGGWCCY 1 cut(s) 319
RsaI GTAC 1 cut(s) 965
RsaNI GTAC 1 cut(s) 964
RseI CAYNNNNRTG 1 cut(s) 770
SaqAI TTAA 2 cut(s) 114, 941
SatI GCNGC 5 cut(s) 276, 555, 658, 749, 1010
Sau96I GGNCC 4 cut(s) 319, 340, 617, 686
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 3 cut(s) 395, 408, 523
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 1 cut(s) 535
SfcI CTRYAG 1 cut(s) 798
SfoI GGCGCC 1 cut(s) 367
Sfr274I CTCGAG 1 cut(s) 260
SfuI TTCGAA 1 cut(s) 1041
SinI GGWCC 4 cut(s) 319, 340, 617, 686
SlaI CTCGAG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 6 cut(s) 127, 402, 889, 929, 954, 1125
SseBI AGGCCT 1 cut(s) 737
SsiI CCGC 5 cut(s) 124, 506, 554, 655, 658
SspDI GGCGCC 1 cut(s) 365
StuI AGGCCT 1 cut(s) 737
StyD4I CCNGG 3 cut(s) 393, 406, 521
StyI CCWWGG 2 cut(s) 195, 420
TaaI ACNGT 2 cut(s) 429, 605
TaiI ACGT 2 cut(s) 473, 627
TaqI TCGA 6 cut(s) 261, 435, 480, 753, 1026, 1041
TaqII GACCGA 1 cut(s) 121
TasI AATT 6 cut(s) 127, 402, 889, 929, 954, 1125
TauI GCSGC 2 cut(s) 557, 660
TfiI GAWTC 1 cut(s) 1067
Tru1I TTAA 2 cut(s) 114, 941
Tru9I TTAA 2 cut(s) 114, 941
TscAI CASTG 2 cut(s) 610, 885
TseFI GTSAC 2 cut(s) 379, 466
TseI GCWGC 3 cut(s) 275, 748, 1009
Tsp45I GTSAC 2 cut(s) 379, 466
TspDTI ATGAA 2 cut(s) 375, 758
TspGWI ACGGA 2 cut(s) 24, 255
TspRI CASTG 2 cut(s) 610, 885
VneI GTGCAC 1 cut(s) 894
VpaK11BI GGWCC 4 cut(s) 319, 340, 617, 686
XagI CCTNNNNNAGG 1 cut(s) 314
XhoI CTCGAG 1 cut(s) 260
XmnI GAANNNNTTC 2 cut(s) 647, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.