Rroxscaffold_5G00376440

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012188
Physical Location & Seq
Reverse (-)
57131354 .. 57133394
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376440.1

Sequence Viewer

Length: 984 bp
ATGGTCGGAAAGAAGGAAACGACGACTTCCTTGGAGGACTTGATAGAGAATGCAGGAGGTTGCGCTGTCGTGGACGGAGGGTTTGCCACACAGCTAGAGAAACACGGCGCTGCTATCAACGACACTCTCTGGAGCGCCGTTTGCCTCATCAAGGACCCCGACCTCATCAAAAGGGTCCACTCGGAATACTTGGAAGCCGGCGCCGATGTTCTGGTCACTTCATCTTACCAGGCAACAATTCCGGGATTTTTGTGCAAGGGACTGTCCATCGAAGAAGGGGAGTCATTGCTGAAGAAGAGTGTGACGTTAGCTGTCGAAGCTCGTAATAGTTTCTGGGAGGCAGTGAAAGTAAGTCCCCGGCATCATCGCTATAACAGGGCTTTGGTTGCGGCCTCTATCGGAAGCTATGGGGCTTATCTTGCTGATGGTTCTGAATACAGTGGGGATTATGGACCAAATGTGGATATGGATAAGCTGAAGGATTTCCACCGCCGCAGATTGCAAGTTCTTGTGGAAGCAGGTCCGGATTTACTTGCATTTGAGACCATTCCTAATAAGCTTGAAGCTCAGGCCTGCGTTGAGCTGCTCGAAGAAGAAAACATTCATATGCCATCTTGGATATGTTTCAGCTCTGTAGATGGTGAGAATGCTCCATCTGGAGAGAACTTCAAGGAATGCCTTGACATAATAAATAGCAGTAACAAAGTAAACGCAGTGGGGATAAATTGTGCACCTCCTCAGTTGATAGAGAGTCTCATCTGCAAATTGAAGCAGTTAACCAGTAAGGCAATTGTTGTGTACCCTAATAGTGGTGAAATATGGGATGGCAAAGCAAAGAGATGGCTGTGCTTCGATGATGACGAGTTCGAATGCTTTGCAACAAGATGGCGCGATTCAGGAGCTAAGCTCATTGGAGGCTGTTGCCGGACAACACCATCAACCATTCAAGCAATTTCTAAGGGTCTAAAGGGAAAATCCCAATGA

Protein Analysis

327

Amino Acids

35.68

Weight (kDa)

5.23

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 23 - 320 3.2e-77 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 200
AccII CGCG 1 cut(s) 891
AccIII TCCGGA 1 cut(s) 523
AciI CCGC 3 cut(s) 389, 490, 493
AcuI CTGAAG 2 cut(s) 311, 497
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 2 cut(s) 151, 809
AgsI TTSAA 4 cut(s) 563, 670, 769, 947
AjnI CCWGG 1 cut(s) 228
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 2 cut(s) 536, 758
Alw44I GTGCAC 1 cut(s) 729
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 2 cut(s) 390, 570
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 2 cut(s) 110, 583
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp700I GAANNNNTTC 2 cut(s) 482, 600
AspLEI GCGC 5 cut(s) 65, 110, 137, 203, 891
AspS9I GGNCC 4 cut(s) 154, 175, 452, 521
AsuC2I CCSGG 2 cut(s) 243, 358
AsuHPI GGTGA 2 cut(s) 653, 824
AsuII TTCGAA 1 cut(s) 867
AvaII GGWCC 4 cut(s) 154, 175, 452, 521
BaeGI GKGCMC 1 cut(s) 733
BanI GGYRCC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 2 cut(s) 97, 570
BccI CCATC 9 cut(s) 275, 419, 619, 632, 661, 818, 834, 879, 943
BceAI ACGGC 2 cut(s) 121, 122
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 1 cut(s) 230
BcnI CCSGG 2 cut(s) 243, 358
BcoDI GTCTC 2 cut(s) 536, 758
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 3 cut(s) 111, 138, 204
BfuAI ACCTGC 1 cut(s) 509
BisI GCNGC 4 cut(s) 111, 390, 493, 584
BlpI GCTNAGC 1 cut(s) 903
BlsI GCNGC 4 cut(s) 112, 391, 494, 585
Bme1390I CCNGG 3 cut(s) 230, 243, 358
Bme18I GGWCC 4 cut(s) 154, 175, 452, 521
BmgT120I GGNCC 4 cut(s) 154, 175, 452, 521
BmiI GGNNCC 3 cut(s) 156, 176, 202
BmrFI CCNGG 3 cut(s) 230, 243, 358
BmsI GCATC 1 cut(s) 370
BplI GAGNNNNNCTC 2 cut(s) 891, 923
BpmI CTGGAG 2 cut(s) 151, 678
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 867
BpuMI CCSGG 2 cut(s) 243, 358
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 2 cut(s) 30, 356
BsaWI WCCGGW 1 cut(s) 523
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 809
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 780
Bse3DI GCAATG 1 cut(s) 284
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 30, 356
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 2 cut(s) 151, 809
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 2 cut(s) 581, 752
BseNI ACTGG 1 cut(s) 780
BseRI GAGGAG 1 cut(s) 726
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 2 cut(s) 97, 570
Bsh1236I CGCG 1 cut(s) 891
BshFI GGCC 2 cut(s) 392, 572
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 5 cut(s) 198, 242, 358, 524, 925
BslFI GGGAC 2 cut(s) 273, 339
BslI CCNNNNNNNGG 2 cut(s) 151, 809
BsmAI GTCTC 2 cut(s) 536, 758
BsmFI GGGAC 2 cut(s) 273, 339
BsmI GAATGC 4 cut(s) 55, 652, 680, 875
BsnI GGCC 2 cut(s) 392, 572
Bso31I GGTCTC 1 cut(s) 536
Bsp119I TTCGAA 1 cut(s) 867
Bsp1286I GDGCHC 1 cut(s) 733
Bsp13I TCCGGA 1 cut(s) 523
Bsp1720I GCTNAGC 1 cut(s) 903
BspACI CCGC 3 cut(s) 389, 490, 493
BspANI GGCC 2 cut(s) 392, 572
BspCNI CTCAG 2 cut(s) 580, 751
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 1 cut(s) 891
BspLI GGNNCC 3 cut(s) 156, 176, 202
BspMI ACCTGC 1 cut(s) 509
BspT104I TTCGAA 1 cut(s) 867
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 536
BsrDI GCAATG 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 1 cut(s) 780
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 30, 356
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 2 cut(s) 264, 440
Bst6I CTCTTC 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 201
BstBI TTCGAA 1 cut(s) 867
BstC8I GCNNGC 2 cut(s) 199, 574
BstDEI CTNAG 4 cut(s) 567, 738, 903, 957
BstENI CCTNNNNNAGG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 829
BstFNI CGCG 1 cut(s) 891
BstH2I RGCGCY 3 cut(s) 111, 138, 204
BstHHI GCGC 5 cut(s) 65, 110, 137, 203, 891
BstMAI GTCTC 2 cut(s) 536, 758
BstMWI GCNNNNNNNGC 4 cut(s) 141, 317, 386, 419
BstNI CCWGG 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 228, 241, 356
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 1 cut(s) 733
BstUI CGCG 1 cut(s) 891
BstV1I GCAGC 2 cut(s) 97, 570
BsuRI GGCC 2 cut(s) 392, 572
BtgZI GCGATG 1 cut(s) 350
BtsCI GGATG 1 cut(s) 829
BtsI GCAGTG 2 cut(s) 348, 720
BtsIMutI CAGTG 3 cut(s) 348, 445, 720
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 2 cut(s) 199, 574
CfoI GCGC 5 cut(s) 65, 110, 137, 203, 891
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 4 cut(s) 154, 175, 452, 521
Csp6I GTAC 1 cut(s) 799
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 4 cut(s) 567, 738, 903, 957
DinI GGCGCC 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 30
Eco147I AGGCCT 1 cut(s) 572
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 4 cut(s) 154, 175, 452, 521
Eco57I CTGAAG 2 cut(s) 311, 497
EcoNI CCTNNNNNAGG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 30
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 30
FaiI YATR 9 cut(s) 372, 408, 450, 467, 606, 608, 622, 686, 820
FaqI GGGAC 2 cut(s) 273, 339
FauNDI CATATG 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 111, 390, 493, 584
FokI GGATG 1 cut(s) 836
Fsp4HI GCNGC 4 cut(s) 111, 390, 493, 584
FspBI CTAG 1 cut(s) 95
GlaI GCGC 5 cut(s) 64, 109, 136, 202, 890
GluI GCNGC 4 cut(s) 111, 390, 493, 584
GsuI CTGGAG 2 cut(s) 151, 678
HaeII RGCGCY 3 cut(s) 111, 138, 204
HaeIII GGCC 2 cut(s) 392, 572
HapII CCGG 5 cut(s) 198, 242, 358, 524, 925
HhaI GCGC 5 cut(s) 65, 110, 137, 203, 891
Hin1I GRCGYC 1 cut(s) 201
Hin6I GCGC 5 cut(s) 63, 108, 135, 201, 889
HinP1I GCGC 5 cut(s) 63, 108, 135, 201, 889
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 3 cut(s) 281, 751, 893
HpaI GTTAAC 1 cut(s) 777
HpaII CCGG 5 cut(s) 198, 242, 358, 524, 925
HphI GGTGA 2 cut(s) 653, 824
Hpy166II GTNNAC 6 cut(s) 73, 178, 709, 731, 777, 799
Hpy188I TCNGA 4 cut(s) 8, 184, 401, 433
Hpy188III TCNNGA 4 cut(s) 130, 524, 657, 897
Hpy8I GTNNAC 6 cut(s) 73, 178, 709, 731, 777, 799
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 3 cut(s) 7, 269, 472
HpyCH4III ACNGT 2 cut(s) 264, 440
HpyCH4IV ACGT 1 cut(s) 305
HpyCH4V TGCA 7 cut(s) 53, 255, 502, 536, 731, 762, 878
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 317, 386, 419
HpyF3I CTNAG 4 cut(s) 567, 738, 903, 957
HpySE526I ACGT 1 cut(s) 305
Hsp92I GRCGYC 1 cut(s) 201
HspAI GCGC 5 cut(s) 63, 108, 135, 201, 889
KasI GGCGCC 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 523
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 777
LmnI GCTCC 3 cut(s) 132, 655, 899
Lsp1109I GCAGC 2 cut(s) 97, 570
LweI GCATC 1 cut(s) 370
MaeI CTAG 1 cut(s) 95
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 3 cut(s) 214, 301, 698
MboII GAAGA 5 cut(s) 284, 304, 307, 602, 605
MfeI CAATTG 1 cut(s) 789
MhlI GDGCHC 1 cut(s) 733
MluCI AATT 5 cut(s) 237, 724, 764, 789, 951
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 2 cut(s) 290, 760
MroI TCCGGA 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 197
MroXI GAANNNNTTC 2 cut(s) 482, 600
MseI TTAA 1 cut(s) 776
MslI CAYNNNNRTG 1 cut(s) 605
MspI CCGG 5 cut(s) 198, 242, 358, 524, 925
MspR9I CCNGG 3 cut(s) 230, 243, 358
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 4 cut(s) 55, 652, 680, 875
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 891
MwoI GCNNNNNNNGC 4 cut(s) 141, 317, 386, 419
NaeI GCCGGC 1 cut(s) 199
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 2 cut(s) 243, 358
NdeI CATATG 1 cut(s) 606
NgoMIV GCCGGC 1 cut(s) 197
NlaIV GGNNCC 3 cut(s) 156, 176, 202
NmuCI GTSAC 2 cut(s) 214, 301
NspV TTCGAA 1 cut(s) 867
PceI AGGCCT 1 cut(s) 572
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PctI GAATGC 4 cut(s) 55, 652, 680, 875
PdiI GCCGGC 1 cut(s) 199
PdmI GAANNNNTTC 2 cut(s) 482, 600
PfeI GAWTC 1 cut(s) 893
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 4 cut(s) 112, 391, 494, 585
PleI GAGTC 2 cut(s) 289, 759
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 2 cut(s) 289, 759
PpuMI RGGWCCY 1 cut(s) 154
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 3 cut(s) 156, 176, 202
PspPI GGNCC 4 cut(s) 154, 175, 452, 521
PspPPI RGGWCCY 1 cut(s) 154
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 1 cut(s) 605
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 4 cut(s) 111, 390, 493, 584
Sau96I GGNCC 4 cut(s) 154, 175, 452, 521
SchI GAGTC 2 cut(s) 290, 760
ScrFI CCNGG 3 cut(s) 230, 243, 358
SduI GDGCHC 1 cut(s) 733
SfaNI GCATC 1 cut(s) 370
SfcI CTRYAG 1 cut(s) 633
SfoI GGCGCC 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 867
SinI GGWCC 4 cut(s) 154, 175, 452, 521
SmiMI CAYNNNNRTG 1 cut(s) 605
Sse9I AATT 5 cut(s) 237, 724, 764, 789, 951
SseBI AGGCCT 1 cut(s) 572
SsiI CCGC 3 cut(s) 389, 490, 493
SspDI GGCGCC 1 cut(s) 200
SspMI CTAG 1 cut(s) 95
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 3 cut(s) 228, 241, 356
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 264, 440
TaiI ACGT 1 cut(s) 308
TaqI TCGA 5 cut(s) 270, 315, 588, 852, 867
TasI AATT 5 cut(s) 237, 724, 764, 789, 951
TauI GCSGC 2 cut(s) 392, 495
TfiI GAWTC 1 cut(s) 893
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 3 cut(s) 348, 445, 720
TseFI GTSAC 2 cut(s) 214, 301
TseI GCWGC 2 cut(s) 110, 583
Tsp45I GTSAC 2 cut(s) 214, 301
TspDTI ATGAA 2 cut(s) 210, 593
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 3 cut(s) 348, 445, 720
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 4 cut(s) 154, 175, 452, 521
XagI CCTNNNNNAGG 1 cut(s) 149
XmnI GAANNNNTTC 2 cut(s) 482, 600
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.