Rorug04G0283300

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012188
Physical Location & Seq
Reverse (-)
45701293 .. 45702158
866 bp
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UTR
Exon/CDS
Intron
Rorug04G0283300.1

Sequence Viewer

Length: 483 bp
ATGGGTGTGTTTACTTATGAAACCGAGTTCACCTCTGTCATCCCACCACCAAGACTCTACAAGGCCTTTGTTCTTGATGCTGATAACCTCATCCCCAAGATTGCCCCACAGGCTGTGAAGAGTGCTGAAATCGTTGAAGGTGATGGAGGTGTTGGAACCATCAAGAAGATCCACCTCGGTGAAGGAAGCGAATACAGCTATGTGAAGCATCAGATTGATGGACTTGACAAAGACAACTTTGTTTACAACTATAGCATTATTGAGGGGGATGCTATAGGAGACAAAGTTGAGAAAATCTCTTACGAGATTAAATTGGTGGCATCTCCAAGTGGAGGTTCCATCATCAAGAGCACCAGCAACTACCATTGCAAAGGTGAGGTTGAGATCAAGGAAGAGCATGTCAAGGCTGGAAAAGAAAGAGCTGCTGGTTTGTTCAAGATCATTGAAAACTACCTTTTGGGCAACCCTGATGCTTACAACTAA

Protein Analysis

160

Amino Acids

17.53

Weight (kDa)

5.24

Isoelectric Point (pI)

18.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 154 9e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 3 cut(s) 137, 436, 446
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 2 cut(s) 198, 422
AluI AGCT 2 cut(s) 198, 422
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 1 cut(s) 163
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 422
AsuHPI GGTGA 4 cut(s) 22, 152, 191, 386
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 1 cut(s) 409
BccI CCATC 4 cut(s) 137, 167, 212, 347
BcoDI GTCTC 1 cut(s) 273
BfmI CTRYAG 2 cut(s) 250, 273
BglI GCCNNNNNGGC 1 cut(s) 110
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 157, 337
BmsI GCATC 5 cut(s) 67, 217, 259, 329, 460
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
BsaJI CCNNGG 1 cut(s) 175
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 3 cut(s) 39, 90, 274
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 364
BseXI GCAGC 1 cut(s) 409
BshFI GGCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 273
BsnI GGCC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 168, 384, 438
BspANI GGCC 1 cut(s) 65
BspLI GGNNCC 2 cut(s) 157, 337
BspPI GGATC 1 cut(s) 163
BspQI GCTCTTC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 3 cut(s) 168, 384, 438
Bst6I CTCTTC 2 cut(s) 113, 387
BstF5I GGATG 3 cut(s) 39, 90, 274
BstKTI GATC 3 cut(s) 171, 387, 441
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 3 cut(s) 168, 384, 438
BstMWI GCNNNNNNNGC 2 cut(s) 110, 195
BstNSI RCATGY 1 cut(s) 401
BstSFI CTRYAG 2 cut(s) 250, 273
BstV1I GCAGC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
BsuRI GGCC 1 cut(s) 65
BtsCI GGATG 3 cut(s) 39, 90, 274
CviAII CATG 1 cut(s) 398
CviJI RGCY 5 cut(s) 65, 113, 198, 407, 422
CviKI_1 RGCY 5 cut(s) 65, 113, 198, 407, 422
DpnI GATC 3 cut(s) 170, 386, 440
DpnII GATC 3 cut(s) 168, 384, 438
Eam1104I CTCTTC 2 cut(s) 113, 387
EarI CTCTTC 2 cut(s) 113, 387
Eco147I AGGCCT 1 cut(s) 65
FaeI CATG 1 cut(s) 401
FaiI YATR 5 cut(s) 18, 201, 252, 275, 399
FatI CATG 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 3 cut(s) 26, 77, 281
Fsp4HI GCNGC 1 cut(s) 423
GluI GCNGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 65
Hin1II CATG 1 cut(s) 401
HinfI GANTC 1 cut(s) 54
HphI GGTGA 4 cut(s) 22, 152, 191, 386
Hpy166II GTNNAC 3 cut(s) 12, 30, 244
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 4 cut(s) 74, 163, 346, 436
Hpy8I GTNNAC 3 cut(s) 12, 30, 244
HpyAV CCTTC 2 cut(s) 131, 176
HpyCH4V TGCA 1 cut(s) 369
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 195
Hsp92II CATG 1 cut(s) 401
Kzo9I GATC 3 cut(s) 168, 384, 438
LguI GCTCTTC 1 cut(s) 387
LpnPI CCDG 4 cut(s) 95, 367, 393, 411
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 5 cut(s) 67, 217, 259, 329, 460
MalI GATC 3 cut(s) 170, 386, 440
MboI GATC 3 cut(s) 168, 384, 438
MboII GAAGA 3 cut(s) 130, 178, 404
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 1 cut(s) 311
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 7 cut(s) 43, 98, 140, 185, 256, 326, 370
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 177
MwoI GCNNNNNNNGC 2 cut(s) 110, 195
NdeII GATC 3 cut(s) 168, 384, 438
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 2 cut(s) 157, 337
NspI RCATGY 1 cut(s) 401
OliI CACNNNNGTG 1 cut(s) 177
PceI AGGCCT 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 387
PkrI GCNGC 1 cut(s) 424
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 157, 337
PsuI RGATCY 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 3 cut(s) 168, 384, 438
SchI GAGTC 1 cut(s) 48
SduI GDGCHC 1 cut(s) 353
SfaNI GCATC 5 cut(s) 67, 217, 259, 329, 460
SfcI CTRYAG 2 cut(s) 250, 273
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 1 cut(s) 311
SseBI AGGCCT 1 cut(s) 65
StuI AGGCCT 1 cut(s) 65
TasI AATT 1 cut(s) 311
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseI GCWGC 1 cut(s) 422
TspDTI ATGAA 1 cut(s) 33
XceI RCATGY 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.