AT3G25910

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006141
Physical Location & Seq
Forward (+)
9489221 .. 9491130
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25910.1

Sequence Viewer

Length: 1119 bp
ATGCCGAAAGAGAGGAAAGAACGGTCCGTATCTCTTGATAAGTATAAACGATCGCCACTGTGTTGTGAGGCAAGCCTTGCTTTGAAGCCGAGTGAGAAACAAGTTAAGGAATGGGAAGAGGCTCGTTGTCCTGTTTGTATGGAACATCCTCACAATGGGATTCTTCTCATTTGTTCTTCTTATGAAAATGGTTGTCGACCTTACATGTGCGACACTAGCCATCGGCATTCTAACTGTTTCGATCAGTTCCGCAAAGCTTCTAAAGAGAAACCTTCTTTGTCTTTACTTCGTGAGGAAGAGGAAAGTAATGAACCAACTGAGATGGAAGATGTGGATTCAGACTCAACTGCTGTAAATCTATTAGGAGAAGCTGCATCTGAGATAACAGTTGTGGATTTAAGTGATGGTGAGAGAGGAGAAGAAGAAGTAGAAGAAGAAGAAGAAGAAGTAGTAGTAGAAGAAGAAGAAGAAGGAATTGTAACAACAGAAGAAGATCAAGAAAAAAACAAACCACAAAAGCTTACTTGTCCGTTATGTCGTGGACATATAAAAGAATGGGTAGTTGTTAAAGCAGCTCGATGTTTCATGAACTCAAAACATAGAAGCTGTTCTTGTGAGACTTGTGATTTCAGTGGAAGCTATTCCGATCTGAGAAAGCACGCAAGGCTTCTACATCCAGGAGTTAGGCCATCAGAAGCAGATCCAGAGAGACAGAGAAGCTGGAGGAGGCTAGAAAGGCAGAGCGATTTAGGGGATTTACTTAGCACACTCCAATCTTCTTTTGGAGGAGATGAAATAAGTAACGATGATGGGTTTCTATTTGCTGATACTCGCTTGCTTACAGTGTACTTTCTCATCCGCGTGTTTAGACCAGAATCGAGTGGCTCAAGGAGTAGTAGCTGGTCTGGTACATCTAGAGCTAGGACACATACAAGTGGGAGAAGAAGATCTTCAAGACCTGCAAGTCTTTGGGGAGAGAGCTATGAAGGTAATACAGGAACATCACCTAGAGATGAGGAGAATAATCAATCTTCTGATGAACAAGTGTCTGGAACTCGGAGACGTCGTTCTAGAAGAAGAACTGTCATTGATGATGATGATGAAGAAGAAGAACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

372

Amino Acids

42.44

Weight (kDa)

5.01

Isoelectric Point (pI)

79.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 39 - 255 4.9e-69 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 963
AatII GACGTC 1 cut(s) 1066
Acc36I ACCTGC 1 cut(s) 967
AccI GTMKAC 1 cut(s) 196
AccII CGCG 1 cut(s) 861
AciI CCGC 2 cut(s) 250, 859
AclWI GGATC 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 1063
AfaI GTAC 2 cut(s) 848, 910
AfiI CCNNNNNNNGG 1 cut(s) 155
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 85, 954
AjnI CCWGG 1 cut(s) 676
AloI GAACNNNNNNTCC 2 cut(s) 1051, 1083
Alw26I GTCTC 3 cut(s) 611, 703, 1054
AlwI GGATC 1 cut(s) 695
AoxI GGCC 1 cut(s) 686
ApeKI GCWGC 2 cut(s) 371, 572
Asp700I GAANNNNTTC 3 cut(s) 607, 640, 949
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 419, 996
AvaII GGWCC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 358, 584
BccI CCATC 5 cut(s) 228, 316, 398, 697, 803
BciT130I CCWGG 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 611, 703, 1054
BfaI CTAG 6 cut(s) 216, 731, 915, 921, 1008, 1071
BfuAI ACCTGC 1 cut(s) 967
BglII AGATCT 1 cut(s) 947
BisI GCNGC 2 cut(s) 372, 573
BlsI GCNGC 2 cut(s) 373, 574
Bme1390I CCNGG 1 cut(s) 678
Bme18I GGWCC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 678
BmsI GCATC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 742
BpuEI CTTGAG 1 cut(s) 871
BsaHI GRCGYC 1 cut(s) 1063
BsaXI ACNNNNNCTCC 2 cut(s) 1051, 1081
Bsc4I CCNNNNNNNGG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 678
BseGI GGATG 3 cut(s) 145, 673, 855
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 3 cut(s) 309, 369, 641
BseRI GAGGAG 4 cut(s) 429, 739, 801, 1031
BseXI GCAGC 2 cut(s) 358, 584
Bsh1236I CGCG 1 cut(s) 861
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 1 cut(s) 688
BsiEI CGRYCG 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 3 cut(s) 611, 703, 1054
BsmBI CGTCTC 1 cut(s) 1054
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 1 cut(s) 688
Bsp143I GATC 6 cut(s) 50, 241, 493, 646, 700, 947
BspACI CCGC 2 cut(s) 250, 859
BspANI GGCC 1 cut(s) 688
BspCNI CTCAG 3 cut(s) 310, 370, 642
BspFNI CGCG 1 cut(s) 861
BspHI TCATGA 1 cut(s) 585
BspMI ACCTGC 1 cut(s) 967
BspPI GGATC 1 cut(s) 695
BssMI GATC 6 cut(s) 50, 241, 493, 646, 700, 947
BssNI GRCGYC 1 cut(s) 1063
Bst2UI CCWGG 1 cut(s) 678
Bst4CI ACNGT 7 cut(s) 24, 60, 236, 388, 844, 1084, 1116
Bst6I CTCTTC 2 cut(s) 111, 291
BstACI GRCGYC 1 cut(s) 1063
BstAPI GCANNNNNTGC 1 cut(s) 77
BstC8I GCNNGC 3 cut(s) 73, 660, 836
BstDEI CTNAG 4 cut(s) 318, 378, 650, 761
BstF5I GGATG 3 cut(s) 145, 673, 855
BstFNI CGCG 1 cut(s) 861
BstKTI GATC 6 cut(s) 53, 244, 496, 649, 703, 950
BstMAI GTCTC 3 cut(s) 611, 703, 1054
BstMBI GATC 6 cut(s) 50, 241, 493, 646, 700, 947
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 4 cut(s) 77, 216, 664, 736
BstNI CCWGG 1 cut(s) 678
BstNSI RCATGY 1 cut(s) 208
BstSCI CCNGG 1 cut(s) 676
BstUI CGCG 1 cut(s) 861
BstV1I GCAGC 2 cut(s) 358, 584
BstX2I RGATCY 2 cut(s) 700, 947
BstYI RGATCY 2 cut(s) 700, 947
BsuRI GGCC 1 cut(s) 688
BtsCI GGATG 3 cut(s) 145, 673, 855
BtsIMutI CAGTG 3 cut(s) 56, 637, 849
BveI ACCTGC 1 cut(s) 967
Cac8I GCNNGC 3 cut(s) 73, 660, 836
CciI TCATGA 1 cut(s) 585
Cfr13I GGNCC 1 cut(s) 24
CpoI CGGWCCG 1 cut(s) 24
Csp6I GTAC 2 cut(s) 847, 909
CspI CGGWCCG 1 cut(s) 24
CviAII CATG 2 cut(s) 205, 586
CviQI GTAC 2 cut(s) 847, 909
DdeI CTNAG 4 cut(s) 318, 378, 650, 761
DpnI GATC 6 cut(s) 52, 243, 495, 648, 702, 949
DpnII GATC 6 cut(s) 50, 241, 493, 646, 700, 947
DrdI GACNNNNNNGTC 1 cut(s) 963
DseDI GACNNNNNNGTC 1 cut(s) 963
Eam1104I CTCTTC 2 cut(s) 111, 291
EarI CTCTTC 2 cut(s) 111, 291
Eco47I GGWCC 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 676
Esp3I CGTCTC 1 cut(s) 1054
FaeI CATG 2 cut(s) 208, 589
FalI AAGNNNNNCTT 6 cut(s) 64, 96, 595, 627, 934, 966
FatI CATG 2 cut(s) 204, 585
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 372, 573
FokI GGATG 3 cut(s) 132, 660, 842
Fsp4HI GCNGC 2 cut(s) 372, 573
FspBI CTAG 6 cut(s) 216, 731, 915, 921, 1008, 1071
GluI GCNGC 2 cut(s) 372, 573
GsuI CTGGAG 1 cut(s) 742
HaeIII GGCC 1 cut(s) 688
Hin1I GRCGYC 1 cut(s) 1063
Hin1II CATG 2 cut(s) 208, 589
HincII GTYRAC 1 cut(s) 197
HindII GTYRAC 1 cut(s) 197
HindIII AAGCTT 2 cut(s) 255, 518
HinfI GANTC 4 cut(s) 160, 335, 341, 875
HphI GGTGA 2 cut(s) 419, 996
Hpy166II GTNNAC 3 cut(s) 197, 542, 847
Hpy188I TCNGA 7 cut(s) 340, 379, 646, 651, 694, 1036, 1059
Hpy188III TCNNGA 9 cut(s) 35, 290, 497, 586, 704, 915, 954, 1050, 1071
Hpy8I GTNNAC 3 cut(s) 197, 542, 847
Hpy99I CGWCG 1 cut(s) 1068
HpyAV CCTTC 3 cut(s) 282, 464, 980
HpyCH4III ACNGT 7 cut(s) 24, 60, 236, 388, 844, 1084, 1116
HpyCH4IV ACGT 1 cut(s) 1063
HpyCH4V TGCA 2 cut(s) 374, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 216, 664, 736
HpyF3I CTNAG 4 cut(s) 318, 378, 650, 761
HpySE526I ACGT 1 cut(s) 1063
Hsp92I GRCGYC 1 cut(s) 1063
Hsp92II CATG 2 cut(s) 208, 589
Kzo9I GATC 6 cut(s) 50, 241, 493, 646, 700, 947
Lsp1109I GCAGC 2 cut(s) 358, 584
LweI GCATC 1 cut(s) 383
MaeI CTAG 6 cut(s) 216, 731, 915, 921, 1008, 1071
MaeII ACGT 1 cut(s) 1063
MaeIII GTNAC 2 cut(s) 478, 800
MalI GATC 6 cut(s) 52, 243, 495, 648, 702, 949
MboI GATC 6 cut(s) 50, 241, 493, 646, 700, 947
MflI RGATCY 2 cut(s) 700, 947
MluCI AATT 1 cut(s) 474
MlyI GAGTC 1 cut(s) 335
MroXI GAANNNNTTC 3 cut(s) 607, 640, 949
MseI TTAA 3 cut(s) 105, 398, 567
MslI CAYNNNNRTG 2 cut(s) 860, 933
MspR9I CCNGG 1 cut(s) 678
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 1 cut(s) 678
MvnI CGCG 1 cut(s) 861
MwoI GCNNNNNNNGC 4 cut(s) 77, 216, 664, 736
NdeII GATC 6 cut(s) 50, 241, 493, 646, 700, 947
NlaIII CATG 2 cut(s) 208, 589
NmeAIII GCCGAG 1 cut(s) 114
NspI RCATGY 1 cut(s) 208
PagI TCATGA 1 cut(s) 585
PciI ACATGT 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 1063
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 3 cut(s) 607, 640, 949
PfeI GAWTC 3 cut(s) 160, 335, 875
PfoI TCCNGGA 1 cut(s) 676
PkrI GCNGC 2 cut(s) 373, 574
Ple19I CGATCG 1 cut(s) 53
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
PscI ACATGT 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 676
PspGI CCWGG 1 cut(s) 676
PspPI GGNCC 1 cut(s) 24
PsuI RGATCY 2 cut(s) 700, 947
PvuI CGATCG 1 cut(s) 53
RsaI GTAC 2 cut(s) 848, 910
RsaNI GTAC 2 cut(s) 847, 909
RseI CAYNNNNRTG 2 cut(s) 860, 933
Rsr2I CGGWCCG 1 cut(s) 24
RsrII CGGWCCG 1 cut(s) 24
SalI GTCGAC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 105, 398, 567
SatI GCNGC 2 cut(s) 372, 573
Sau3AI GATC 6 cut(s) 50, 241, 493, 646, 700, 947
Sau96I GGNCC 1 cut(s) 24
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 1 cut(s) 678
SfaNI GCATC 1 cut(s) 383
SinI GGWCC 1 cut(s) 24
SmiMI CAYNNNNRTG 2 cut(s) 860, 933
SmlI CTYRAG 1 cut(s) 886
SmoI CTYRAG 1 cut(s) 886
Sse9I AATT 1 cut(s) 474
SsiI CCGC 2 cut(s) 250, 859
SspMI CTAG 6 cut(s) 216, 731, 915, 921, 1008, 1071
StyD4I CCNGG 1 cut(s) 676
TaaI ACNGT 7 cut(s) 24, 60, 236, 388, 844, 1084, 1116
TaiI ACGT 1 cut(s) 1066
TaqI TCGA 4 cut(s) 196, 240, 577, 878
TasI AATT 1 cut(s) 474
TatI WGTACW 1 cut(s) 846
TfiI GAWTC 3 cut(s) 160, 335, 875
Tru1I TTAA 3 cut(s) 105, 398, 567
Tru9I TTAA 3 cut(s) 105, 398, 567
TscAI CASTG 3 cut(s) 63, 637, 849
TseI GCWGC 2 cut(s) 371, 572
TspDTI ATGAA 8 cut(s) 198, 324, 574, 602, 807, 999, 1053, 1116
TspGWI ACGGA 2 cut(s) 16, 519
TspRI CASTG 3 cut(s) 63, 637, 849
VpaK11BI GGWCC 1 cut(s) 24
XbaI TCTAGA 2 cut(s) 914, 1070
XceI RCATGY 1 cut(s) 208
XcmI CCANNNNNNNNNTGG 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 196
XmnI GAANNNNTTC 3 cut(s) 607, 640, 949
XspI CTAG 6 cut(s) 216, 731, 915, 921, 1008, 1071
ZraI GACGTC 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.