RchiOBHm_Chr2g0089221

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006141
Physical Location & Seq
Forward (+)
3482208 .. 3484890
2683 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46452

Sequence Viewer

Length: 1047 bp
ATGCCTAAGGAAAGAAGGGACCGTTCTGTGTCTCGAGAGAGGTACAGAGCATCTCCTTATCCATGCAGCTCCAGTCATACAAAGCGTCCCTCACTCAAAATTCCTTCAGAAACTGCAGAAAATGTGAAAGAGTGGGAAGAAGCCAGGTGCCCAGTTTGTTTGGAACATCCACACAATGCAGTTCTGCTCATATGTTCATCTTTTGAGAAGGGGTGCCGCCCTTACATGTGTGACACAAGCTACCGCCATTCAAACTGTCTTGATCAGTTCTGCAAGTCATTTTCAGAAGAAATCTCACCAGAGATGCCGCCAGAAGAAGACATTCAGCTTTCAGATACTCAGCTGCTTCCATTTGAAACTTCTGAATCAATAATTACTGATGTGCAAAGTGTTGCAACTGAGGAAGAACCGCCAACCATGATTCCCATATCTTGTGAACACCAGGAACAGCCCAAGCTTGTGTGCCCTCTCTGCCGTGGGGAGGTGAAAGATTGGATTATTGTGGAGCCTGCTCGTTGTTTCATGAATGCAAAATCAAGAAACTGTTCTTGTGAGACGTGCAGTTTTAGCGGAACGTATACAGATCTCAGAAAGCATGCTAGGCTGGAGCACCCGCAAGCACGGCCATCAGAGGCTGACCCAGAGCGACAGCGTTCCTGGAGGAGTTTGGAGCGACAGAGGGACATTGGTGATCTGCTGAGCACACTCCAATCTTCTATGGGAGAGGATAGGGCTGATGACAGCAGCTTGGCAGTAGAACAGGATGGGGGATGGCTAACTGTATTCTTCCTAGTTAGAATCTACCGACCTTCTGGGTCCAGTTCGAGGAGCAGCAGTTGGTCTCCTACCACAAGAACTAGAGCACAAATTAGTATGAGGAGGAGGGCTACCAGACTTTGGGGAGAAAGCTACGAGGGTGAAGCTGCGTCGTCTTCAAGAGGAGAAGCCGACAATGACTCCTCAGATGGTGGCTCAGCCCCTACTAGGAGGCAGAGTGCGCGTCTCCGGCAAAGGCGACGGACAAGCCCGGACAACAACCAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

348

Amino Acids

39.59

Weight (kDa)

6.07

Isoelectric Point (pI)

75.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 46 - 234 1e-70 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 147, 213
AccB7I CCANNNNNTGG 1 cut(s) 897
AccI GTMKAC 1 cut(s) 578
AccII CGCG 1 cut(s) 1000
AciI CCGC 6 cut(s) 217, 244, 308, 410, 570, 614
AcoI YGGCCR 1 cut(s) 623
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 4 cut(s) 481, 825, 897, 984
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 252, 356, 936
AjiI CACGTC 1 cut(s) 558
AjnI CCWGG 3 cut(s) 143, 441, 656
AluBI AGCT 8 cut(s) 69, 240, 328, 343, 457, 747, 909, 923
AluI AGCT 8 cut(s) 69, 240, 328, 343, 457, 747, 909, 923
Alw21I GWGCWC 3 cut(s) 612, 704, 865
Alw26I GTCTC 4 cut(s) 36, 548, 846, 1007
AlwNI CAGNNNCTG 2 cut(s) 113, 635
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 1 cut(s) 623
ApeKI GCWGC 5 cut(s) 66, 343, 744, 831, 923
ApoI RAATTY 1 cut(s) 99
Asp700I GAANNNNTTC 2 cut(s) 321, 544
AspLEI GCGC 1 cut(s) 1000
AspS9I GGNCC 2 cut(s) 19, 816
AsuC2I CCSGG 1 cut(s) 1028
AsuHPI GGTGA 4 cut(s) 288, 496, 701, 929
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 2 cut(s) 19, 816
AxyI CCTNAGG 1 cut(s) 6
BaeGI GKGCMC 2 cut(s) 152, 467
BanI GGYRCC 2 cut(s) 147, 213
BbsI GAAGAC 2 cut(s) 324, 924
Bbv12I GWGCWC 3 cut(s) 612, 704, 865
BbvI GCAGC 5 cut(s) 78, 330, 756, 843, 910
BccI CCATC 4 cut(s) 634, 758, 765, 959
BceAI ACGGC 2 cut(s) 459, 638
BciT130I CCWGG 3 cut(s) 145, 443, 658
BclI TGATCA 1 cut(s) 262
BcnI CCSGG 1 cut(s) 1028
BcoDI GTCTC 4 cut(s) 36, 548, 846, 1007
BfaI CTAG 4 cut(s) 600, 791, 858, 984
BfmI CTRYAG 1 cut(s) 114
BglII AGATCT 1 cut(s) 583
BisI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 832, 924
BlpI GCTNAGC 2 cut(s) 698, 973
BlsI GCNGC 7 cut(s) 68, 218, 309, 345, 746, 833, 925
Bme1390I CCNGG 4 cut(s) 145, 443, 658, 1028
Bme18I GGWCC 2 cut(s) 19, 816
BmeT110I CYCGRG 1 cut(s) 33
BmgBI CACGTC 1 cut(s) 558
BmgT120I GGNCC 2 cut(s) 19, 816
BmiI GGNNCC 5 cut(s) 20, 149, 215, 507, 817
BmrFI CCNGG 4 cut(s) 145, 443, 658, 1028
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 2 cut(s) 59, 294
BmuI ACTGGG 1 cut(s) 146
BpiI GAAGAC 2 cut(s) 324, 924
BpmI CTGGAG 3 cut(s) 55, 626, 679
Bpu1102I GCTNAGC 2 cut(s) 698, 973
BpuMI CCSGG 1 cut(s) 1028
BsaI GGTCTC 1 cut(s) 846
BsaJI CCNNGG 1 cut(s) 475
BsaXI ACNNNNNCTCC 6 cut(s) 820, 850, 941, 971, 979, 1009
Bsc4I CCNNNNNNNGG 4 cut(s) 481, 825, 897, 984
Bse1I ACTGG 3 cut(s) 72, 152, 819
Bse21I CCTNAGG 1 cut(s) 6
BseBI CCWGG 3 cut(s) 145, 443, 658
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 3 cut(s) 166, 769, 776
BseLI CCNNNNNNNGG 4 cut(s) 481, 825, 897, 984
BseMII CTCAG 6 cut(s) 353, 390, 601, 689, 975, 987
BseNI ACTGG 3 cut(s) 72, 152, 819
BseRI GAGGAG 6 cut(s) 676, 841, 892, 895, 949, 954
BseSI GKGCMC 2 cut(s) 152, 467
BseXI GCAGC 5 cut(s) 78, 330, 756, 843, 910
BsgI GTGCAG 1 cut(s) 580
Bsh1236I CGCG 1 cut(s) 1000
BshFI GGCC 1 cut(s) 625
BshNI GGYRCC 2 cut(s) 147, 213
BsiHKAI GWGCWC 3 cut(s) 612, 704, 865
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 2 cut(s) 1006, 1028
BslFI GGGAC 3 cut(s) 32, 72, 695
BslI CCNNNNNNNGG 4 cut(s) 481, 825, 897, 984
BsmAI GTCTC 4 cut(s) 36, 548, 846, 1007
BsmBI CGTCTC 2 cut(s) 548, 1007
BsmFI GGGAC 3 cut(s) 32, 72, 695
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 1 cut(s) 625
Bso31I GGTCTC 1 cut(s) 846
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 5 cut(s) 152, 467, 612, 704, 865
Bsp143I GATC 3 cut(s) 262, 583, 691
Bsp1720I GCTNAGC 2 cut(s) 698, 973
BspACI CCGC 6 cut(s) 217, 244, 308, 410, 570, 614
BspANI GGCC 1 cut(s) 625
BspCNI CTCAG 6 cut(s) 352, 391, 600, 690, 974, 986
BspFNI CGCG 1 cut(s) 1000
BspHI TCATGA 1 cut(s) 522
BspLI GGNNCC 5 cut(s) 20, 149, 215, 507, 817
BspMAI CTGCAG 1 cut(s) 118
BspT107I GGYRCC 2 cut(s) 147, 213
BspTNI GGTCTC 1 cut(s) 846
BsrI ACTGG 3 cut(s) 72, 152, 819
BssECI CCNNGG 1 cut(s) 475
BssMI GATC 3 cut(s) 262, 583, 691
BssNAI GTATAC 1 cut(s) 579
Bst1107I GTATAC 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 145, 443, 658
Bst4CI ACNGT 4 cut(s) 23, 257, 545, 781
BstC8I GCNNGC 3 cut(s) 510, 597, 618
BstDEI CTNAG 7 cut(s) 6, 339, 399, 587, 698, 961, 973
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 3 cut(s) 166, 769, 776
BstFNI CGCG 1 cut(s) 1000
BstHHI GCGC 1 cut(s) 1000
BstKTI GATC 3 cut(s) 265, 586, 694
BstMAI GTCTC 4 cut(s) 36, 548, 846, 1007
BstMBI GATC 3 cut(s) 262, 583, 691
BstMWI GCNNNNNNNGC 6 cut(s) 471, 567, 601, 622, 997, 1006
BstNI CCWGG 3 cut(s) 145, 443, 658
BstNSI RCATGY 2 cut(s) 229, 599
BstSCI CCNGG 4 cut(s) 143, 441, 656, 1026
BstSFI CTRYAG 1 cut(s) 114
BstSLI GKGCMC 2 cut(s) 152, 467
BstUI CGCG 1 cut(s) 1000
BstV1I GCAGC 5 cut(s) 78, 330, 756, 843, 910
BstV2I GAAGAC 2 cut(s) 324, 924
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZ17I GTATAC 1 cut(s) 579
Bsu36I CCTNAGG 1 cut(s) 6
BsuRI GGCC 1 cut(s) 625
BtgI CCRYGG 1 cut(s) 475
BtrI CACGTC 1 cut(s) 558
BtsCI GGATG 3 cut(s) 166, 769, 776
Cac8I GCNNGC 3 cut(s) 510, 597, 618
CaiI CAGNNNCTG 2 cut(s) 113, 635
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 1000
Cfr13I GGNCC 2 cut(s) 19, 816
CseI GACGC 3 cut(s) 74, 915, 989
Csp6I GTAC 1 cut(s) 43
CviAII CATG 6 cut(s) 63, 226, 418, 523, 596, 1044
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 7 cut(s) 6, 339, 399, 587, 698, 961, 973
DpnI GATC 3 cut(s) 264, 585, 693
DpnII GATC 3 cut(s) 262, 583, 691
EaeI YGGCCR 1 cut(s) 623
Eco31I GGTCTC 1 cut(s) 846
Eco47I GGWCC 2 cut(s) 19, 816
Eco57I CTGAAG 1 cut(s) 90
Eco81I CCTNAGG 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 33
EcoRII CCWGG 3 cut(s) 143, 441, 656
Esp3I CGTCTC 2 cut(s) 548, 1007
FaeI CATG 6 cut(s) 66, 229, 421, 526, 599, 1047
FaqI GGGAC 3 cut(s) 32, 72, 695
FatI CATG 6 cut(s) 62, 225, 417, 522, 595, 1043
FauI CCCGC 1 cut(s) 621
FauNDI CATATG 1 cut(s) 191
FbaI TGATCA 1 cut(s) 262
FblI GTMKAC 1 cut(s) 578
Fnu4HI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 832, 924
FokI GGATG 3 cut(s) 153, 776, 783
Fsp4HI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 832, 924
FspBI CTAG 4 cut(s) 600, 791, 858, 984
GlaI GCGC 1 cut(s) 999
GluI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 832, 924
GsuI CTGGAG 3 cut(s) 55, 626, 679
HaeIII GGCC 1 cut(s) 625
HapII CCGG 2 cut(s) 1006, 1028
HgaI GACGC 3 cut(s) 74, 915, 989
HhaI GCGC 1 cut(s) 1000
Hin1II CATG 6 cut(s) 66, 229, 421, 526, 599, 1047
Hin6I GCGC 1 cut(s) 998
HinP1I GCGC 1 cut(s) 998
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 4 cut(s) 365, 421, 798, 956
HpaII CCGG 2 cut(s) 1006, 1028
HphI GGTGA 4 cut(s) 288, 496, 701, 929
Hpy166II GTNNAC 2 cut(s) 437, 579
Hpy188I TCNGA 7 cut(s) 109, 286, 334, 364, 590, 631, 964
Hpy188III TCNNGA 6 cut(s) 33, 35, 260, 523, 537, 936
Hpy8I GTNNAC 2 cut(s) 437, 579
Hpy99I CGWCG 2 cut(s) 931, 1020
HpyAV CCTTC 4 cut(s) 9, 114, 202, 819
HpyCH4III ACNGT 4 cut(s) 23, 257, 545, 781
HpyCH4IV ACGT 2 cut(s) 557, 575
HpyCH4V TGCA 8 cut(s) 66, 116, 179, 273, 385, 395, 530, 561
HpyF10VI GCNNNNNNNGC 6 cut(s) 471, 567, 601, 622, 997, 1006
HpyF3I CTNAG 7 cut(s) 6, 339, 399, 587, 698, 961, 973
HpySE526I ACGT 2 cut(s) 557, 575
Hsp92II CATG 6 cut(s) 66, 229, 421, 526, 599, 1047
HspAI GCGC 1 cut(s) 998
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 3 cut(s) 262, 583, 691
LmnI GCTCC 5 cut(s) 74, 505, 607, 670, 828
Lsp1109I GCAGC 5 cut(s) 78, 330, 756, 843, 910
LweI GCATC 2 cut(s) 59, 294
MaeI CTAG 4 cut(s) 600, 791, 858, 984
MaeII ACGT 2 cut(s) 557, 575
MaeIII GTNAC 1 cut(s) 230
MalI GATC 3 cut(s) 264, 585, 693
MboI GATC 3 cut(s) 262, 583, 691
MboII GAAGA 8 cut(s) 149, 299, 326, 329, 416, 705, 778, 924
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 5 cut(s) 152, 467, 612, 704, 865
MluCI AATT 3 cut(s) 99, 372, 867
MlyI GAGTC 1 cut(s) 950
MroXI GAANNNNTTC 2 cut(s) 321, 544
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 2 cut(s) 1006, 1028
MspR9I CCNGG 4 cut(s) 145, 443, 658, 1028
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 3 cut(s) 145, 443, 658
MvnI CGCG 1 cut(s) 1000
MwoI GCNNNNNNNGC 6 cut(s) 471, 567, 601, 622, 997, 1006
NciI CCSGG 1 cut(s) 1028
NdeI CATATG 1 cut(s) 191
NdeII GATC 3 cut(s) 262, 583, 691
NlaIII CATG 6 cut(s) 66, 229, 421, 526, 599, 1047
NlaIV GGNNCC 5 cut(s) 20, 149, 215, 507, 817
NmuCI GTSAC 1 cut(s) 230
NspI RCATGY 2 cut(s) 229, 599
PaeI GCATGC 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 33
PagI TCATGA 1 cut(s) 522
PciI ACATGT 1 cut(s) 225
PctI GAATGC 1 cut(s) 532
PdmI GAANNNNTTC 2 cut(s) 321, 544
PfeI GAWTC 3 cut(s) 365, 421, 798
PflMI CCANNNNNTGG 1 cut(s) 897
PfoI TCCNGGA 1 cut(s) 656
PkrI GCNGC 7 cut(s) 68, 218, 309, 345, 746, 833, 925
PleI GAGTC 1 cut(s) 950
PpsI GAGTC 1 cut(s) 950
PscI ACATGT 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 143, 441, 656
PspGI CCWGG 3 cut(s) 143, 441, 656
PspN4I GGNNCC 5 cut(s) 20, 149, 215, 507, 817
PspPI GGNCC 2 cut(s) 19, 816
PstI CTGCAG 1 cut(s) 118
PstNI CAGNNNCTG 2 cut(s) 113, 635
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SatI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 832, 924
Sau3AI GATC 3 cut(s) 262, 583, 691
Sau96I GGNCC 2 cut(s) 19, 816
SchI GAGTC 1 cut(s) 950
ScrFI CCNGG 4 cut(s) 145, 443, 658, 1028
SduI GDGCHC 5 cut(s) 152, 467, 612, 704, 865
SfaNI GCATC 2 cut(s) 59, 294
SfcI CTRYAG 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 33
SinI GGWCC 2 cut(s) 19, 816
SlaI CTCGAG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 33
SmoI CTYRAG 1 cut(s) 33
SphI GCATGC 1 cut(s) 599
Sse9I AATT 3 cut(s) 99, 372, 867
SsiI CCGC 6 cut(s) 217, 244, 308, 410, 570, 614
SspMI CTAG 4 cut(s) 600, 791, 858, 984
StyD4I CCNGG 4 cut(s) 143, 441, 656, 1026
TaaI ACNGT 4 cut(s) 23, 257, 545, 781
TaiI ACGT 2 cut(s) 560, 578
TaqI TCGA 2 cut(s) 34, 824
TasI AATT 3 cut(s) 99, 372, 867
TauI GCSGC 2 cut(s) 219, 310
TfiI GAWTC 3 cut(s) 365, 421, 798
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 5 cut(s) 66, 343, 744, 831, 923
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 186, 511, 539
TspGWI ACGGA 1 cut(s) 1033
Van91I CCANNNNNTGG 1 cut(s) 897
VpaK11BI GGWCC 2 cut(s) 19, 816
XapI RAATTY 1 cut(s) 99
XceI RCATGY 2 cut(s) 229, 599
XhoI CTCGAG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 578
XmnI GAANNNNTTC 2 cut(s) 321, 544
XspI CTAG 4 cut(s) 600, 791, 858, 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.