pycom15g15830

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006141
Physical Location & Seq
Forward (+)
11042112 .. 11043152
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g15830.1

Sequence Viewer

Length: 1041 bp
ATGCCTAAGGATAGGAGGGGCCGTTCTGTGACTCCTGATAGGTATAGAGCATCTCCTTATCCATGCAGCTCCAGTCATACAAGGTGGTCGTCACCCAGAATTCCTTCAGAAACTGAGGACAATGTGAAAGAATGGGAAGAAGCCAGATGCCCCGTTTGCCTGGAGCATCCACACAATGCAGTTCTCCTAATATGTTCATCGTATGAACAAGGGTGCCGCCCTTACATGTGTGACACGAGCTACCGCCATTCAAATTGTCTAGACCAGTACTGCAAGTCATTTTCGGCAGAAACCTCACCAACTATCCCACCAGAAGGAGCAACTCAGATATCAGATACTCAGTTGTCTCCATCTGCAACTCTTGAATCAACAATTACTCAAGTGCAAAACAATCGTACAATTGAGGAGGTGCTCTCCTCCATGAATGCCGTATCTTGTGAGCATCAGGCTGATCCGAAGCTCGTGTGCCCACTCTGCCGTGGGGAGATAACAGATTGGATTATTGTTGAGTCTGCTCGTTGTTTCATGAACGCGAAATCAAGAAATTGTTCTTGTGAGACATGTAATTATAGCGGAACCTATGCAGATCTCAGGAAGCATGCAAGGCTGGAGCATCCACTAGTGTGCCCATCAGAGGCAGATCCAGAGCGACAGCGTACCTGGAGGAGTTTGGAGCGACAGAGGGATATTGGCGACTTGTTCAGCTCAATCCAATCTTCAGTTGGAGAGGATAGGGGTGATGATAGCAGTAGTTTGCCGGCTGATGACGGTGCGGGCTGGCTAACCATATTCTTTCTAGTTAGAGTAGTTCGACCCGGGTCCAGTTCAAGGAGCAGCAGCTGGTCTGGTACCACAAGAACCAGAGCACAAGTTAGTATGAGAAGGAGAGCAACCAGGCTTTGGGGGGAGAGCTACGAGGGCGAAGCAGCATCTTCTACTCTAGAAGAGGTTAACGAGTCTTCAGATGGCGACTCGGGTGTTAGGAGGAGACGCAGTGCGCGCCTCCGGCGATGGACAACACCGGACAACAACCAGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

347

Amino Acids

38.93

Weight (kDa)

6.21

Isoelectric Point (pI)

64.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 46 - 235 6.4e-67 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 848
AccB1I GGYRCC 2 cut(s) 213, 848
AccB7I CCANNNNNTGG 1 cut(s) 900
AccII CGCG 2 cut(s) 533, 1000
AciI CCGC 4 cut(s) 217, 244, 573, 773
AclWI GGATC 2 cut(s) 446, 635
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 3 cut(s) 90, 702, 945
AfaI GTAC 4 cut(s) 269, 397, 658, 850
AfiI CCNNNNNNNGG 4 cut(s) 314, 634, 828, 900
AflIII ACRYGT 2 cut(s) 225, 560
AgsI TTSAA 3 cut(s) 252, 365, 828
AhlI ACTAGT 1 cut(s) 619
AjnI CCWGG 3 cut(s) 159, 659, 893
AleI CACNNNNGTG 1 cut(s) 622
AloI GAACNNNNNNTCC 2 cut(s) 7, 39
AluBI AGCT 6 cut(s) 69, 240, 460, 705, 840, 912
AluI AGCT 6 cut(s) 69, 240, 460, 705, 840, 912
Alw21I GWGCWC 2 cut(s) 414, 868
Alw26I GTCTC 3 cut(s) 351, 551, 982
AlwI GGATC 2 cut(s) 446, 635
AlwNI CAGNNNCTG 2 cut(s) 113, 840
Ama87I CYCGRG 2 cut(s) 815, 973
AoxI GGCC 1 cut(s) 19
ApeKI GCWGC 4 cut(s) 66, 834, 837, 926
ApoI RAATTY 1 cut(s) 99
Asp700I GAANNNNTTC 2 cut(s) 103, 547
Asp718I GGTACC 1 cut(s) 848
AspLEI GCGC 2 cut(s) 1000, 1002
AspS9I GGNCC 2 cut(s) 19, 819
AsuC2I CCSGG 2 cut(s) 816, 817
AsuHPI GGTGA 3 cut(s) 84, 288, 749
AvaI CYCGRG 2 cut(s) 815, 973
AvaII GGWCC 1 cut(s) 819
AxyI CCTNAGG 1 cut(s) 6
BaeGI GKGCMC 2 cut(s) 470, 629
BanI GGYRCC 2 cut(s) 213, 848
BauI CACGAG 2 cut(s) 235, 461
BbsI GAAGAC 1 cut(s) 951
Bbv12I GWGCWC 2 cut(s) 414, 868
BbvI GCAGC 4 cut(s) 78, 846, 849, 938
BccI CCATC 4 cut(s) 358, 637, 959, 1005
BceAI ACGGC 4 cut(s) 6, 413, 462, 1021
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 3 cut(s) 161, 661, 895
BcnI CCSGG 2 cut(s) 816, 817
BcoDI GTCTC 3 cut(s) 351, 551, 982
BcuI ACTAGT 1 cut(s) 619
BfaI CTAG 4 cut(s) 260, 620, 797, 941
BglII AGATCT 1 cut(s) 586
BisI GCNGC 5 cut(s) 67, 217, 835, 838, 927
BlsI GCNGC 5 cut(s) 68, 218, 836, 839, 928
BmcAI AGTACT 1 cut(s) 269
Bme1390I CCNGG 5 cut(s) 161, 661, 816, 817, 895
Bme18I GGWCC 1 cut(s) 819
BmeT110I CYCGRG 2 cut(s) 815, 973
BmgT120I GGNCC 2 cut(s) 19, 819
BmiI GGNNCC 5 cut(s) 20, 215, 577, 820, 850
BmrFI CCNGG 5 cut(s) 161, 661, 816, 817, 895
BmsI GCATC 6 cut(s) 59, 137, 175, 451, 622, 938
BoxI GACNNNNGTC 1 cut(s) 817
BpiI GAAGAC 1 cut(s) 951
BplI GAGNNNNNCTC 2 cut(s) 398, 430
BpmI CTGGAG 4 cut(s) 55, 182, 629, 682
BpuEI CTTGAG 1 cut(s) 363
BpuMI CCSGG 2 cut(s) 816, 817
BsaJI CCNNGG 2 cut(s) 478, 815
BsaWI WCCGGW 1 cut(s) 1021
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 979, 1009
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 634, 828, 900
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 3 cut(s) 72, 265, 822
Bse21I CCTNAGG 1 cut(s) 6
BseBI CCWGG 3 cut(s) 161, 661, 895
BseDI CCNNGG 2 cut(s) 478, 815
BseGI GGATG 2 cut(s) 166, 613
BseLI CCNNNNNNNGG 4 cut(s) 314, 634, 828, 900
BseMII CTCAG 4 cut(s) 105, 338, 353, 604
BseNI ACTGG 3 cut(s) 72, 265, 822
BsePI GCGCGC 1 cut(s) 998
BseRI GAGGAG 4 cut(s) 406, 419, 679, 1000
BseSI GKGCMC 2 cut(s) 470, 629
BseXI GCAGC 4 cut(s) 78, 846, 849, 938
Bsh1236I CGCG 2 cut(s) 533, 1000
BshFI GGCC 1 cut(s) 21
BshNI GGYRCC 2 cut(s) 213, 848
BsiHKAI GWGCWC 2 cut(s) 414, 868
BsiHKCI CYCGRG 2 cut(s) 815, 973
BsiSI CCGG 4 cut(s) 758, 816, 1006, 1022
BslI CCNNNNNNNGG 4 cut(s) 314, 634, 828, 900
BsmAI GTCTC 3 cut(s) 351, 551, 982
BsmBI CGTCTC 1 cut(s) 982
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 1 cut(s) 21
BsoBI CYCGRG 2 cut(s) 815, 973
Bsp1286I GDGCHC 4 cut(s) 414, 470, 629, 868
Bsp143I GATC 3 cut(s) 451, 586, 640
BspACI CCGC 4 cut(s) 217, 244, 573, 773
BspANI GGCC 1 cut(s) 21
BspCNI CTCAG 4 cut(s) 106, 337, 352, 603
BspFNI CGCG 2 cut(s) 533, 1000
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 5 cut(s) 20, 215, 577, 820, 850
BspPI GGATC 2 cut(s) 446, 635
BspT107I GGYRCC 2 cut(s) 213, 848
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 3 cut(s) 72, 265, 822
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 2 cut(s) 478, 815
BssHII GCGCGC 1 cut(s) 998
BssMI GATC 3 cut(s) 451, 586, 640
BssSI CACGAG 2 cut(s) 235, 461
Bst2BI CACGAG 2 cut(s) 235, 461
Bst2UI CCWGG 3 cut(s) 161, 661, 895
Bst4CI ACNGT 1 cut(s) 770
Bst6I CTCTTC 1 cut(s) 939
BstC8I GCNNGC 5 cut(s) 600, 759, 775, 779, 1000
BstDEI CTNAG 5 cut(s) 6, 114, 324, 339, 590
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 166, 613
BstFNI CGCG 2 cut(s) 533, 1000
BstHHI GCGC 2 cut(s) 1000, 1002
BstKTI GATC 3 cut(s) 454, 589, 643
BstMAI GTCTC 3 cut(s) 351, 551, 982
BstMBI GATC 3 cut(s) 451, 586, 640
BstMWI GCNNNNNNNGC 6 cut(s) 156, 474, 604, 918, 999, 1006
BstNI CCWGG 3 cut(s) 161, 661, 895
BstNSI RCATGY 3 cut(s) 229, 564, 602
BstPAI GACNNNNGTC 1 cut(s) 817
BstSCI CCNGG 5 cut(s) 159, 659, 814, 815, 893
BstSLI GKGCMC 2 cut(s) 470, 629
BstUI CGCG 2 cut(s) 533, 1000
BstV1I GCAGC 4 cut(s) 78, 846, 849, 938
BstV2I GAAGAC 1 cut(s) 951
BstX2I RGATCY 2 cut(s) 586, 640
BstYI RGATCY 2 cut(s) 586, 640
Bsu36I CCTNAGG 1 cut(s) 6
BsuRI GGCC 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 478
BtgZI GCGATG 1 cut(s) 1024
BtsCI GGATG 2 cut(s) 166, 613
BtsI GCAGTG 1 cut(s) 1000
BtsIMutI CAGTG 1 cut(s) 1000
Cac8I GCNNGC 5 cut(s) 600, 759, 775, 779, 1000
CaiI CAGNNNCTG 2 cut(s) 113, 840
CciI TCATGA 1 cut(s) 525
CfoI GCGC 2 cut(s) 1000, 1002
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 2 cut(s) 19, 819
Cfr9I CCCGGG 1 cut(s) 815
CseI GACGC 1 cut(s) 999
Csp6I GTAC 4 cut(s) 268, 396, 657, 849
CviAII CATG 6 cut(s) 63, 226, 421, 526, 561, 599
CviQI GTAC 4 cut(s) 268, 396, 657, 849
DdeI CTNAG 5 cut(s) 6, 114, 324, 339, 590
DpnI GATC 3 cut(s) 453, 588, 642
DpnII GATC 3 cut(s) 451, 586, 640
Eam1104I CTCTTC 1 cut(s) 939
EarI CTCTTC 1 cut(s) 939
Eco32I GATATC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 819
Eco57I CTGAAG 3 cut(s) 90, 702, 945
Eco81I CCTNAGG 1 cut(s) 6
Eco88I CYCGRG 2 cut(s) 815, 973
EcoRI GAATTC 1 cut(s) 99
EcoRII CCWGG 3 cut(s) 159, 659, 893
EcoRV GATATC 1 cut(s) 330
Esp3I CGTCTC 1 cut(s) 982
FaeI CATG 6 cut(s) 66, 229, 424, 529, 564, 602
FatI CATG 6 cut(s) 62, 225, 420, 525, 560, 598
FauI CCCGC 1 cut(s) 766
Fnu4HI GCNGC 5 cut(s) 67, 217, 835, 838, 927
FokI GGATG 2 cut(s) 153, 600
Fsp4HI GCNGC 5 cut(s) 67, 217, 835, 838, 927
FspBI CTAG 4 cut(s) 260, 620, 797, 941
GlaI GCGC 2 cut(s) 999, 1001
GluI GCNGC 5 cut(s) 67, 217, 835, 838, 927
GsuI CTGGAG 4 cut(s) 55, 182, 629, 682
HaeIII GGCC 1 cut(s) 21
HapII CCGG 4 cut(s) 758, 816, 1006, 1022
HgaI GACGC 1 cut(s) 999
HhaI GCGC 2 cut(s) 1000, 1002
Hin1II CATG 6 cut(s) 66, 229, 424, 529, 564, 602
Hin6I GCGC 2 cut(s) 998, 1000
HinP1I GCGC 2 cut(s) 998, 1000
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 5 cut(s) 31, 365, 509, 956, 971
HpaI GTTAAC 1 cut(s) 952
HpaII CCGG 4 cut(s) 758, 816, 1006, 1022
HphI GGTGA 3 cut(s) 84, 288, 749
Hpy166II GTNNAC 1 cut(s) 952
Hpy188I TCNGA 6 cut(s) 109, 327, 334, 456, 634, 964
Hpy188III TCNNGA 8 cut(s) 35, 260, 362, 526, 540, 592, 644, 941
Hpy8I GTNNAC 1 cut(s) 952
HpyAV CCTTC 3 cut(s) 114, 308, 876
HpyCH4III ACNGT 1 cut(s) 770
HpyCH4V TGCA 7 cut(s) 66, 179, 273, 356, 385, 584, 602
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 474, 604, 918, 999, 1006
HpyF3I CTNAG 5 cut(s) 6, 114, 324, 339, 590
Hsp92II CATG 6 cut(s) 66, 229, 424, 529, 564, 602
HspAI GCGC 2 cut(s) 998, 1000
KpnI GGTACC 1 cut(s) 852
KroI GCCGGC 1 cut(s) 757
KroNI GCCGGC 1 cut(s) 759
KspAI GTTAAC 1 cut(s) 952
Kzo9I GATC 3 cut(s) 451, 586, 640
LmnI GCTCC 6 cut(s) 74, 163, 317, 610, 673, 831
Lsp1109I GCAGC 4 cut(s) 78, 846, 849, 938
LweI GCATC 6 cut(s) 59, 137, 175, 451, 622, 938
MaeI CTAG 4 cut(s) 260, 620, 797, 941
MaeIII GTNAC 3 cut(s) 28, 90, 230
MalI GATC 3 cut(s) 453, 588, 642
MboI GATC 3 cut(s) 451, 586, 640
MboII GAAGA 5 cut(s) 149, 708, 924, 951, 956
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 2 cut(s) 586, 640
MhlI GDGCHC 4 cut(s) 414, 470, 629, 868
MluCI AATT 6 cut(s) 99, 253, 372, 399, 544, 565
MlyI GAGTC 4 cut(s) 25, 518, 965, 965
MmeI TCCRAC 1 cut(s) 703
MroNI GCCGGC 1 cut(s) 757
MroXI GAANNNNTTC 2 cut(s) 103, 547
MseI TTAA 1 cut(s) 951
MslI CAYNNNNRTG 1 cut(s) 622
MspA1I CMGCKG 1 cut(s) 840
MspI CCGG 4 cut(s) 758, 816, 1006, 1022
MspR9I CCNGG 5 cut(s) 161, 661, 816, 817, 895
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 3 cut(s) 161, 661, 895
MvnI CGCG 2 cut(s) 533, 1000
MwoI GCNNNNNNNGC 6 cut(s) 156, 474, 604, 918, 999, 1006
NaeI GCCGGC 1 cut(s) 759
NciI CCSGG 2 cut(s) 816, 817
NdeII GATC 3 cut(s) 451, 586, 640
NgoMIV GCCGGC 1 cut(s) 757
NlaIII CATG 6 cut(s) 66, 229, 424, 529, 564, 602
NlaIV GGNNCC 5 cut(s) 20, 215, 577, 820, 850
NmuCI GTSAC 3 cut(s) 28, 90, 230
NspI RCATGY 3 cut(s) 229, 564, 602
OliI CACNNNNGTG 1 cut(s) 622
PaeI GCATGC 1 cut(s) 602
PagI TCATGA 1 cut(s) 525
PauI GCGCGC 1 cut(s) 998
PciI ACATGT 2 cut(s) 225, 560
PctI GAATGC 1 cut(s) 430
PdiI GCCGGC 1 cut(s) 759
PdmI GAANNNNTTC 2 cut(s) 103, 547
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 900
PkrI GCNGC 5 cut(s) 68, 218, 836, 839, 928
PleI GAGTC 4 cut(s) 25, 517, 964, 965
PpsI GAGTC 4 cut(s) 25, 517, 964, 965
PscI ACATGT 2 cut(s) 225, 560
PshAI GACNNNNGTC 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 159, 659, 893
PspGI CCWGG 3 cut(s) 159, 659, 893
PspN4I GGNNCC 5 cut(s) 20, 215, 577, 820, 850
PspPI GGNCC 2 cut(s) 19, 819
PstNI CAGNNNCTG 2 cut(s) 113, 840
PsuI RGATCY 2 cut(s) 586, 640
PteI GCGCGC 1 cut(s) 998
PvuII CAGCTG 1 cut(s) 840
RsaI GTAC 4 cut(s) 269, 397, 658, 850
RsaNI GTAC 4 cut(s) 268, 396, 657, 849
RseI CAYNNNNRTG 1 cut(s) 622
SaqAI TTAA 1 cut(s) 951
SatI GCNGC 5 cut(s) 67, 217, 835, 838, 927
Sau3AI GATC 3 cut(s) 451, 586, 640
Sau96I GGNCC 2 cut(s) 19, 819
ScaI AGTACT 1 cut(s) 269
SchI GAGTC 4 cut(s) 25, 518, 965, 965
ScrFI CCNGG 5 cut(s) 161, 661, 816, 817, 895
SduI GDGCHC 4 cut(s) 414, 470, 629, 868
SfaNI GCATC 6 cut(s) 59, 137, 175, 451, 622, 938
SinI GGWCC 1 cut(s) 819
SmaI CCCGGG 1 cut(s) 817
SmiMI CAYNNNNRTG 1 cut(s) 622
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SpeI ACTAGT 1 cut(s) 619
SphI GCATGC 1 cut(s) 602
Sse9I AATT 6 cut(s) 99, 253, 372, 399, 544, 565
SsiI CCGC 4 cut(s) 217, 244, 573, 773
SspMI CTAG 4 cut(s) 260, 620, 797, 941
StyD4I CCNGG 5 cut(s) 159, 659, 814, 815, 893
TaaI ACNGT 1 cut(s) 770
TaqI TCGA 1 cut(s) 811
TasI AATT 6 cut(s) 99, 253, 372, 399, 544, 565
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 1 cut(s) 1000
TseFI GTSAC 3 cut(s) 28, 90, 230
TseI GCWGC 4 cut(s) 66, 834, 837, 926
Tsp45I GTSAC 3 cut(s) 28, 90, 230
TspDTI ATGAA 5 cut(s) 186, 219, 437, 514, 542
TspMI CCCGGG 1 cut(s) 815
TspRI CASTG 1 cut(s) 1000
Van91I CCANNNNNTGG 1 cut(s) 900
VpaK11BI GGWCC 1 cut(s) 819
XapI RAATTY 1 cut(s) 99
XbaI TCTAGA 2 cut(s) 259, 940
XceI RCATGY 3 cut(s) 229, 564, 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 719
XmaI CCCGGG 1 cut(s) 815
XmnI GAANNNNTTC 2 cut(s) 103, 547
XspI CTAG 4 cut(s) 260, 620, 797, 941
ZrmI AGTACT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.