MD02G1036700.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006141
Physical Location & Seq
Forward (+)
2783806 .. 2786680
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1036700.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGCCTAAGGATAGGAGGGGACGTTCTGTTTCTCGTGATCGGTATAGAGCATCTCCTTATCCATGCAGCTCCAGTCCTACAAGGCGGTTGTTGCCCAAAATTCCTTCAGAAACTGAGGACAATTTGAAAGAATGGGAAGAAGCCAGATGCCCCGTTTGCTTGGAGCATCCACACAATGCTGTTCTCCTAATATGTTCATCCTATGGGAAAGGGTGCCGCCCTTACATGTGTGACACAAGCTACCGCCATTCAAACTGTCTAGACCAGTACTGCAAGTCATTTTCGGCAGAAACCTCACCAACTATCCCACCAGAAGGAGCAACTCAGATTTCAGATACTCAGTTGTCTCCATCTGAAACTCTTGAATCAACAATTACTCAAGTGCAAAATGAGAGTACAATTGAGGATGAGCTCTCCTCCATGAATTCCGTATCTTGTGAGCATCAGGCAGAGCCGAAGCTTGTGTGCCCCCTCTGCCGTGGGGAGATAACAGATTGGATTATTGTGGAGCCTGCTCGTTGTTTCATGAATGCAAAATCAAGAAATTGTTCTTGTGAGACATGTAATTATAACGGAACCTATGCAGATCTCAGGAAGCATGCGAGGCTGGAGCATCCATTAGTGCGCCCATCAGAGGCAGACCCAGAGCGACAGCGTAGCTGGAGGAGTTTGGAGCGGCAGAGGGATATTGCTGACCTGTTCAGCTCAATCCAATCTTCAGTTGGAGAGGATAGGGGTGATGATAGCAGTAGTTTGCCGGCTGATGACGGTGCGGGCTGGCTAACCATATTCTTTCTAGTTAGAGTAGTTCGACCTGGGTCCAGTTCGAGGAGCAGCAGCTGGTATGGAACCACAAGAACCAGAGCACAAGTTAGTATGAGAAGGAGAGCAAACAGACTATGGGGGGAGAGCTACGAGGGCGAAGCTGCATCTTCAACCGTAGAAGAGGATAACGAGTCTTCAGACGGTGACTCAGGTGTTAGGAGGCGCAGTGCGCGCCTCAGGCGACGGACAACACCAGACAACAACCAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

346

Amino Acids

38.92

Weight (kDa)

6.15

Isoelectric Point (pI)

66.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 46 - 235 1e-67 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 676
AccII CGCG 1 cut(s) 997
AciI CCGC 5 cut(s) 85, 217, 244, 676, 773
AcsI RAATTY 2 cut(s) 99, 424
AcuI CTGAAG 3 cut(s) 90, 702, 945
AfaI GTAC 2 cut(s) 269, 397
AfiI CCNNNNNNNGG 3 cut(s) 314, 634, 828
AflIII ACRYGT 2 cut(s) 225, 560
AgsI TTSAA 4 cut(s) 127, 252, 365, 936
AjnI CCWGG 1 cut(s) 814
AloI GAACNNNNNNTCC 2 cut(s) 7, 39
AluBI AGCT 9 cut(s) 69, 240, 412, 460, 660, 705, 840, 912, 926
AluI AGCT 9 cut(s) 69, 240, 412, 460, 660, 705, 840, 912, 926
Alw21I GWGCWC 2 cut(s) 414, 868
Alw26I GTCTC 2 cut(s) 351, 551
AlwNI CAGNNNCTG 2 cut(s) 113, 840
ApeKI GCWGC 4 cut(s) 66, 834, 837, 926
ApoI RAATTY 2 cut(s) 99, 424
Asp700I GAANNNNTTC 1 cut(s) 547
AspLEI GCGC 4 cut(s) 627, 990, 997, 999
AspS9I GGNCC 1 cut(s) 819
AsuHPI GGTGA 3 cut(s) 288, 749, 980
AvaII GGWCC 1 cut(s) 819
AxyI CCTNAGG 2 cut(s) 6, 1001
BaeGI GKGCMC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 213
BanII GRGCYC 1 cut(s) 414
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 951
Bbv12I GWGCWC 2 cut(s) 414, 868
BbvI GCAGC 4 cut(s) 78, 846, 849, 913
BccI CCATC 2 cut(s) 358, 637
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 1 cut(s) 816
BcoDI GTCTC 2 cut(s) 351, 551
BfaI CTAG 2 cut(s) 260, 797
BglII AGATCT 1 cut(s) 586
BisI GCNGC 6 cut(s) 67, 217, 677, 835, 838, 927
BlsI GCNGC 6 cut(s) 68, 218, 678, 836, 839, 928
BmcAI AGTACT 1 cut(s) 269
Bme1390I CCNGG 1 cut(s) 816
Bme18I GGWCC 1 cut(s) 819
BmgT120I GGNCC 1 cut(s) 819
BmiI GGNNCC 5 cut(s) 215, 510, 577, 820, 850
BmrFI CCNGG 1 cut(s) 816
BmsI GCATC 6 cut(s) 59, 137, 175, 451, 622, 938
BoxI GACNNNNGTC 1 cut(s) 817
BpiI GAAGAC 1 cut(s) 951
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 3 cut(s) 55, 629, 682
BpuEI CTTGAG 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 478, 815
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 976, 1006
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 634, 828
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 3 cut(s) 72, 265, 822
Bse21I CCTNAGG 2 cut(s) 6, 1001
BseBI CCWGG 1 cut(s) 816
BseDI CCNNGG 2 cut(s) 478, 815
BseGI GGATG 4 cut(s) 166, 197, 412, 613
BseLI CCNNNNNNNGG 3 cut(s) 314, 634, 828
BseMII CTCAG 6 cut(s) 105, 338, 353, 604, 987, 1015
BseNI ACTGG 3 cut(s) 72, 265, 822
BsePI GCGCGC 1 cut(s) 995
BseRI GAGGAG 3 cut(s) 406, 679, 844
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 4 cut(s) 78, 846, 849, 913
Bsh1236I CGCG 1 cut(s) 997
BshNI GGYRCC 1 cut(s) 213
BsiHKAI GWGCWC 2 cut(s) 414, 868
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 3 cut(s) 314, 634, 828
BsmAI GTCTC 2 cut(s) 351, 551
BsmFI GGGAC 1 cut(s) 33
BsmI GAATGC 1 cut(s) 535
Bsp1286I GDGCHC 3 cut(s) 414, 470, 868
Bsp143I GATC 2 cut(s) 37, 586
BspACI CCGC 5 cut(s) 85, 217, 244, 676, 773
BspCNI CTCAG 6 cut(s) 106, 337, 352, 603, 986, 1014
BspFNI CGCG 1 cut(s) 997
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 5 cut(s) 215, 510, 577, 820, 850
BspT107I GGYRCC 1 cut(s) 213
BsrBI CCGCTC 1 cut(s) 676
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 3 cut(s) 72, 265, 822
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 2 cut(s) 478, 815
BssHII GCGCGC 1 cut(s) 995
BssMI GATC 2 cut(s) 37, 586
BssSI CACGAG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 4 cut(s) 257, 770, 940, 968
Bst6I CTCTTC 1 cut(s) 939
BstC8I GCNNGC 6 cut(s) 513, 600, 759, 775, 779, 997
BstDEI CTNAG 7 cut(s) 6, 114, 324, 339, 590, 973, 1001
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 4 cut(s) 166, 197, 412, 613
BstFNI CGCG 1 cut(s) 997
BstHHI GCGC 4 cut(s) 627, 990, 997, 999
BstKTI GATC 2 cut(s) 40, 589
BstMAI GTCTC 2 cut(s) 351, 551
BstMBI GATC 2 cut(s) 37, 586
BstMWI GCNNNNNNNGC 8 cut(s) 91, 156, 474, 604, 918, 994, 996, 1003
BstNI CCWGG 1 cut(s) 816
BstNSI RCATGY 3 cut(s) 229, 564, 602
BstPAI GACNNNNGTC 1 cut(s) 817
BstSCI CCNGG 1 cut(s) 814
BstSLI GKGCMC 1 cut(s) 470
BstUI CGCG 1 cut(s) 997
BstV1I GCAGC 4 cut(s) 78, 846, 849, 913
BstV2I GAAGAC 1 cut(s) 951
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
Bsu36I CCTNAGG 2 cut(s) 6, 1001
BtgI CCRYGG 1 cut(s) 478
BtsCI GGATG 4 cut(s) 166, 197, 412, 613
BtsI GCAGTG 1 cut(s) 997
BtsIMutI CAGTG 1 cut(s) 997
Cac8I GCNNGC 6 cut(s) 513, 600, 759, 775, 779, 997
CaiI CAGNNNCTG 2 cut(s) 113, 840
CciI TCATGA 1 cut(s) 525
CfoI GCGC 4 cut(s) 627, 990, 997, 999
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 1 cut(s) 819
Csp6I GTAC 2 cut(s) 268, 396
CviAII CATG 7 cut(s) 63, 226, 421, 526, 561, 599, 1035
CviQI GTAC 2 cut(s) 268, 396
DdeI CTNAG 7 cut(s) 6, 114, 324, 339, 590, 973, 1001
DpnI GATC 2 cut(s) 39, 588
DpnII GATC 2 cut(s) 37, 586
Eam1104I CTCTTC 1 cut(s) 939
EarI CTCTTC 1 cut(s) 939
Ecl136II GAGCTC 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 819
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 3 cut(s) 90, 702, 945
Eco81I CCTNAGG 2 cut(s) 6, 1001
EcoICRI GAGCTC 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 814
EcoT38I GRGCYC 1 cut(s) 414
FaeI CATG 7 cut(s) 66, 229, 424, 529, 564, 602, 1038
FaqI GGGAC 1 cut(s) 33
FatI CATG 7 cut(s) 62, 225, 420, 525, 560, 598, 1034
FauI CCCGC 1 cut(s) 766
Fnu4HI GCNGC 6 cut(s) 67, 217, 677, 835, 838, 927
FokI GGATG 4 cut(s) 153, 184, 419, 600
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 6 cut(s) 67, 217, 677, 835, 838, 927
FspBI CTAG 2 cut(s) 260, 797
GlaI GCGC 4 cut(s) 626, 989, 996, 998
GluI GCNGC 6 cut(s) 67, 217, 677, 835, 838, 927
GsuI CTGGAG 3 cut(s) 55, 629, 682
HapII CCGG 1 cut(s) 758
HhaI GCGC 4 cut(s) 627, 990, 997, 999
Hin1II CATG 7 cut(s) 66, 229, 424, 529, 564, 602, 1038
Hin6I GCGC 4 cut(s) 625, 988, 995, 997
HinP1I GCGC 4 cut(s) 625, 988, 995, 997
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 3 cut(s) 365, 956, 971
HpaII CCGG 1 cut(s) 758
HphI GGTGA 3 cut(s) 288, 749, 980
Hpy188I TCNGA 6 cut(s) 109, 327, 334, 355, 634, 964
Hpy188III TCNNGA 6 cut(s) 35, 260, 362, 526, 540, 592
Hpy99I CGWCG 1 cut(s) 1011
HpyAV CCTTC 3 cut(s) 114, 308, 876
HpyCH4III ACNGT 4 cut(s) 257, 770, 940, 968
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 6 cut(s) 66, 273, 385, 533, 584, 929
HpyF10VI GCNNNNNNNGC 8 cut(s) 91, 156, 474, 604, 918, 994, 996, 1003
HpyF3I CTNAG 7 cut(s) 6, 114, 324, 339, 590, 973, 1001
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 7 cut(s) 66, 229, 424, 529, 564, 602, 1038
HspAI GCGC 4 cut(s) 625, 988, 995, 997
KroI GCCGGC 1 cut(s) 757
KroNI GCCGGC 1 cut(s) 759
Kzo9I GATC 2 cut(s) 37, 586
LmnI GCTCC 7 cut(s) 74, 163, 317, 508, 610, 673, 831
Lsp1109I GCAGC 4 cut(s) 78, 846, 849, 913
LweI GCATC 6 cut(s) 59, 137, 175, 451, 622, 938
MaeI CTAG 2 cut(s) 260, 797
MaeII ACGT 1 cut(s) 22
MaeIII GTNAC 2 cut(s) 230, 968
MalI GATC 2 cut(s) 39, 588
MbiI CCGCTC 1 cut(s) 676
MboI GATC 2 cut(s) 37, 586
MboII GAAGA 5 cut(s) 149, 708, 924, 951, 956
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 3 cut(s) 414, 470, 868
MluCI AATT 7 cut(s) 99, 121, 372, 399, 424, 544, 565
MlyI GAGTC 2 cut(s) 965, 965
MmeI TCCRAC 1 cut(s) 703
MroNI GCCGGC 1 cut(s) 757
MroXI GAANNNNTTC 1 cut(s) 547
MspA1I CMGCKG 1 cut(s) 840
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 816
MunI CAATTG 1 cut(s) 399
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 1 cut(s) 816
MvnI CGCG 1 cut(s) 997
MwoI GCNNNNNNNGC 8 cut(s) 91, 156, 474, 604, 918, 994, 996, 1003
NaeI GCCGGC 1 cut(s) 759
NdeII GATC 2 cut(s) 37, 586
NgoMIV GCCGGC 1 cut(s) 757
NlaIII CATG 7 cut(s) 66, 229, 424, 529, 564, 602, 1038
NlaIV GGNNCC 5 cut(s) 215, 510, 577, 820, 850
NmuCI GTSAC 2 cut(s) 230, 968
NspI RCATGY 3 cut(s) 229, 564, 602
PaeI GCATGC 1 cut(s) 602
PagI TCATGA 1 cut(s) 525
PauI GCGCGC 1 cut(s) 995
PciI ACATGT 2 cut(s) 225, 560
PctI GAATGC 1 cut(s) 535
PdiI GCCGGC 1 cut(s) 759
PdmI GAANNNNTTC 1 cut(s) 547
PfeI GAWTC 1 cut(s) 365
PkrI GCNGC 6 cut(s) 68, 218, 678, 836, 839, 928
PleI GAGTC 2 cut(s) 964, 965
PpsI GAGTC 2 cut(s) 964, 965
PscI ACATGT 2 cut(s) 225, 560
PshAI GACNNNNGTC 1 cut(s) 817
PsiI TTATAA 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 814
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 5 cut(s) 215, 510, 577, 820, 850
PspPI GGNCC 1 cut(s) 819
PstNI CAGNNNCTG 2 cut(s) 113, 840
PsuI RGATCY 1 cut(s) 586
PteI GCGCGC 1 cut(s) 995
PvuII CAGCTG 1 cut(s) 840
RsaI GTAC 2 cut(s) 269, 397
RsaNI GTAC 2 cut(s) 268, 396
SacI GAGCTC 1 cut(s) 414
SatI GCNGC 6 cut(s) 67, 217, 677, 835, 838, 927
Sau3AI GATC 2 cut(s) 37, 586
Sau96I GGNCC 1 cut(s) 819
ScaI AGTACT 1 cut(s) 269
SchI GAGTC 2 cut(s) 965, 965
ScrFI CCNGG 1 cut(s) 816
SduI GDGCHC 3 cut(s) 414, 470, 868
SfaNI GCATC 6 cut(s) 59, 137, 175, 451, 622, 938
SinI GGWCC 1 cut(s) 819
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SphI GCATGC 1 cut(s) 602
Sse9I AATT 7 cut(s) 99, 121, 372, 399, 424, 544, 565
SsiI CCGC 5 cut(s) 85, 217, 244, 676, 773
SspMI CTAG 2 cut(s) 260, 797
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 814
TaaI ACNGT 4 cut(s) 257, 770, 940, 968
TaiI ACGT 1 cut(s) 25
TaqI TCGA 2 cut(s) 811, 827
TasI AATT 7 cut(s) 99, 121, 372, 399, 424, 544, 565
TatI WGTACW 2 cut(s) 267, 395
TauI GCSGC 2 cut(s) 219, 679
TfiI GAWTC 1 cut(s) 365
TscAI CASTG 1 cut(s) 997
TseFI GTSAC 2 cut(s) 230, 968
TseI GCWGC 4 cut(s) 66, 834, 837, 926
Tsp45I GTSAC 2 cut(s) 230, 968
TspDTI ATGAA 4 cut(s) 186, 437, 514, 542
TspGWI ACGGA 3 cut(s) 418, 588, 1024
TspRI CASTG 1 cut(s) 997
VpaK11BI GGWCC 1 cut(s) 819
XapI RAATTY 2 cut(s) 99, 424
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 3 cut(s) 229, 564, 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 719
XmnI GAANNNNTTC 1 cut(s) 547
XspI CTAG 2 cut(s) 260, 797
ZrmI AGTACT 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.