Rh2AG044300

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006141
Physical Location & Seq
Forward (+)
3413025 .. 3415861
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG044300.1

Sequence Viewer

Length: 1050 bp
ATGCCTAAGGAAAGAAGGGACCGTTCTGTGTCTCGAGAGAGGTACAGAGCATCTCCTTATCCATGCAGCTCCAGTCATACAAAGCGTCCCTCACTCAAAATTCCTTCAGAAACTGCAGACAATGTGAAAGAGTGGGAAGAAGCCAGGTGCCCAGTTTGTTTGGAACATCCACACAATGCAGTTCTGCTCATATGTTCATCTTTTGAGAAGGGGTGCCGCCCTTACATGTGTGACACAAGCTACCGCCATTCAAACTGTCTTGATCAGTTCTGCAAGTCATTTTCAGAAGAAATCTCACCAGAGATGCCGCCAGAAGAAGACATTCAGCTTTCAGATACTCAGCTGCTTCCATTTGAAACTTCTGAATCAATAATTACTGATGTGCAAAGTGTTGCAACTGAGGAAGAACCGCCAACCATGATTCCCATATCCTGTGAACACCAGGAACAGCCCAAGCTTGTGTGCCCTCTCTGCCGTGGGGAGGTGAAAGATTGGATTATTGTGGAGCCTGCTCGTTGTTTCATGAATGCAAAATCAAGAAACTGTTCTTGTGAGACGTGCAGTTTTAGCGGAACCTATACAGATCTCAGAAAGCATGCAAGGCTGGAGCACCCGCAAGCACGCCCATCAGAGGCTGACCCAGAGCGACAGCGTTCCTGGAGGAGTTTGGAGCGACAGAGGGACATTGGTGATCTGCTGAGCACACTCCAATCTTCTATGGGAGAGGATAGGGCTGATGACAGCAGCTTGGCAGTAGAACAGGATGGGGGATGGCTAACTGTATTCTTCCTAGTTAGAATCTACCGACCTACGTCTGGGTCCAGTTCGAGGAGCAGCAGTTGGTCTCCTACCACAAGAACTAGAGCACAAATTAGTATGAGAAGGAGGGCTACCAGACTTTGGGGAGAAAGCTACGAGGGTGAAGCTGCGTCGTCTTCAAGAGGAGAAGCCGACAATGACTCCTCAGATGGTGGCTCAGCCCCTACTAGGAGGCAGAGTGCGCGTCTCCGGCAAAGGCGACGGACAAGCCCAGACAACAACCAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

349

Amino Acids

39.68

Weight (kDa)

6.07

Isoelectric Point (pI)

74.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 46 - 234 1e-70 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 147, 213
AccB7I CCANNNNNTGG 1 cut(s) 900
AccII CGCG 1 cut(s) 1003
AciI CCGC 6 cut(s) 217, 244, 308, 410, 570, 614
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 6 cut(s) 481, 631, 815, 828, 900, 987
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 252, 356, 939
AjiI CACGTC 1 cut(s) 558
AjnI CCWGG 3 cut(s) 143, 441, 656
AluBI AGCT 8 cut(s) 69, 240, 328, 343, 457, 747, 912, 926
AluI AGCT 8 cut(s) 69, 240, 328, 343, 457, 747, 912, 926
Alw21I GWGCWC 3 cut(s) 612, 704, 868
Alw26I GTCTC 4 cut(s) 36, 548, 849, 1010
AlwNI CAGNNNCTG 2 cut(s) 113, 635
Ama87I CYCGRG 1 cut(s) 33
ApeKI GCWGC 5 cut(s) 66, 343, 744, 834, 926
ApoI RAATTY 1 cut(s) 99
Asp700I GAANNNNTTC 2 cut(s) 321, 544
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 2 cut(s) 19, 819
AsuHPI GGTGA 4 cut(s) 288, 496, 701, 932
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 2 cut(s) 19, 819
AxyI CCTNAGG 1 cut(s) 6
BaeGI GKGCMC 2 cut(s) 152, 467
BanI GGYRCC 2 cut(s) 147, 213
BarI GAAGNNNNNNTAC 2 cut(s) 874, 906
BbsI GAAGAC 2 cut(s) 324, 927
Bbv12I GWGCWC 3 cut(s) 612, 704, 868
BbvI GCAGC 5 cut(s) 78, 330, 756, 846, 913
BccI CCATC 4 cut(s) 634, 758, 765, 962
BceAI ACGGC 1 cut(s) 459
BciT130I CCWGG 3 cut(s) 145, 443, 658
BclI TGATCA 1 cut(s) 262
BcoDI GTCTC 4 cut(s) 36, 548, 849, 1010
BfaI CTAG 3 cut(s) 791, 861, 987
BfmI CTRYAG 1 cut(s) 114
BglII AGATCT 1 cut(s) 583
BisI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 835, 927
BlpI GCTNAGC 2 cut(s) 698, 976
BlsI GCNGC 7 cut(s) 68, 218, 309, 345, 746, 836, 928
Bme1390I CCNGG 3 cut(s) 145, 443, 658
Bme18I GGWCC 2 cut(s) 19, 819
BmeT110I CYCGRG 1 cut(s) 33
BmgBI CACGTC 1 cut(s) 558
BmgT120I GGNCC 2 cut(s) 19, 819
BmiI GGNNCC 6 cut(s) 20, 149, 215, 507, 574, 820
BmrFI CCNGG 3 cut(s) 145, 443, 658
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 2 cut(s) 59, 294
BmuI ACTGGG 1 cut(s) 146
BoxI GACNNNNGTC 1 cut(s) 811
BpiI GAAGAC 2 cut(s) 324, 927
BpmI CTGGAG 3 cut(s) 55, 626, 679
Bpu1102I GCTNAGC 2 cut(s) 698, 976
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 1 cut(s) 475
BsaXI ACNNNNNCTCC 6 cut(s) 823, 853, 944, 974, 982, 1012
Bsc4I CCNNNNNNNGG 6 cut(s) 481, 631, 815, 828, 900, 987
Bse1I ACTGG 3 cut(s) 72, 152, 822
Bse21I CCTNAGG 1 cut(s) 6
BseBI CCWGG 3 cut(s) 145, 443, 658
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 3 cut(s) 166, 769, 776
BseLI CCNNNNNNNGG 6 cut(s) 481, 631, 815, 828, 900, 987
BseMII CTCAG 6 cut(s) 353, 390, 601, 689, 978, 990
BseNI ACTGG 3 cut(s) 72, 152, 822
BseRI GAGGAG 4 cut(s) 676, 844, 952, 957
BseSI GKGCMC 2 cut(s) 152, 467
BseXI GCAGC 5 cut(s) 78, 330, 756, 846, 913
BsgI GTGCAG 1 cut(s) 580
Bsh1236I CGCG 1 cut(s) 1003
BshNI GGYRCC 2 cut(s) 147, 213
BsiHKAI GWGCWC 3 cut(s) 612, 704, 868
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 1 cut(s) 1009
BslFI GGGAC 3 cut(s) 32, 72, 695
BslI CCNNNNNNNGG 6 cut(s) 481, 631, 815, 828, 900, 987
BsmAI GTCTC 4 cut(s) 36, 548, 849, 1010
BsmBI CGTCTC 2 cut(s) 548, 1010
BsmFI GGGAC 3 cut(s) 32, 72, 695
BsmI GAATGC 1 cut(s) 532
Bso31I GGTCTC 1 cut(s) 849
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 5 cut(s) 152, 467, 612, 704, 868
Bsp143I GATC 3 cut(s) 262, 583, 691
Bsp1720I GCTNAGC 2 cut(s) 698, 976
BspACI CCGC 6 cut(s) 217, 244, 308, 410, 570, 614
BspCNI CTCAG 6 cut(s) 352, 391, 600, 690, 977, 989
BspFNI CGCG 1 cut(s) 1003
BspHI TCATGA 1 cut(s) 522
BspLI GGNNCC 6 cut(s) 20, 149, 215, 507, 574, 820
BspMAI CTGCAG 1 cut(s) 118
BspT107I GGYRCC 2 cut(s) 147, 213
BspTNI GGTCTC 1 cut(s) 849
BsrI ACTGG 3 cut(s) 72, 152, 822
BssECI CCNNGG 1 cut(s) 475
BssMI GATC 3 cut(s) 262, 583, 691
Bst2UI CCWGG 3 cut(s) 145, 443, 658
Bst4CI ACNGT 4 cut(s) 23, 257, 545, 781
BstC8I GCNNGC 4 cut(s) 510, 597, 618, 622
BstDEI CTNAG 7 cut(s) 6, 339, 399, 587, 698, 964, 976
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 3 cut(s) 166, 769, 776
BstFNI CGCG 1 cut(s) 1003
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 3 cut(s) 265, 586, 694
BstMAI GTCTC 4 cut(s) 36, 548, 849, 1010
BstMBI GATC 3 cut(s) 262, 583, 691
BstMWI GCNNNNNNNGC 5 cut(s) 471, 567, 601, 1000, 1009
BstNI CCWGG 3 cut(s) 145, 443, 658
BstNSI RCATGY 2 cut(s) 229, 599
BstPAI GACNNNNGTC 1 cut(s) 811
BstSCI CCNGG 3 cut(s) 143, 441, 656
BstSFI CTRYAG 1 cut(s) 114
BstSLI GKGCMC 2 cut(s) 152, 467
BstUI CGCG 1 cut(s) 1003
BstV1I GCAGC 5 cut(s) 78, 330, 756, 846, 913
BstV2I GAAGAC 2 cut(s) 324, 927
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
Bsu36I CCTNAGG 1 cut(s) 6
BtgI CCRYGG 1 cut(s) 475
BtrI CACGTC 1 cut(s) 558
BtsCI GGATG 3 cut(s) 166, 769, 776
Cac8I GCNNGC 4 cut(s) 510, 597, 618, 622
CaiI CAGNNNCTG 2 cut(s) 113, 635
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 1003
Cfr13I GGNCC 2 cut(s) 19, 819
CseI GACGC 3 cut(s) 74, 918, 992
Csp6I GTAC 1 cut(s) 43
CviAII CATG 6 cut(s) 63, 226, 418, 523, 596, 1047
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 7 cut(s) 6, 339, 399, 587, 698, 964, 976
DpnI GATC 3 cut(s) 264, 585, 693
DpnII GATC 3 cut(s) 262, 583, 691
Eco31I GGTCTC 1 cut(s) 849
Eco47I GGWCC 2 cut(s) 19, 819
Eco57I CTGAAG 1 cut(s) 90
Eco81I CCTNAGG 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 33
EcoRII CCWGG 3 cut(s) 143, 441, 656
Esp3I CGTCTC 2 cut(s) 548, 1010
FaeI CATG 6 cut(s) 66, 229, 421, 526, 599, 1050
FaqI GGGAC 3 cut(s) 32, 72, 695
FatI CATG 6 cut(s) 62, 225, 417, 522, 595, 1046
FauI CCCGC 1 cut(s) 621
FauNDI CATATG 1 cut(s) 191
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 835, 927
FokI GGATG 3 cut(s) 153, 776, 783
Fsp4HI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 835, 927
FspBI CTAG 3 cut(s) 791, 861, 987
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 835, 927
GsuI CTGGAG 3 cut(s) 55, 626, 679
HapII CCGG 1 cut(s) 1009
HgaI GACGC 3 cut(s) 74, 918, 992
HhaI GCGC 1 cut(s) 1003
Hin1II CATG 6 cut(s) 66, 229, 421, 526, 599, 1050
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 4 cut(s) 365, 421, 798, 959
HpaII CCGG 1 cut(s) 1009
HphI GGTGA 4 cut(s) 288, 496, 701, 932
Hpy166II GTNNAC 1 cut(s) 437
Hpy188I TCNGA 7 cut(s) 109, 286, 334, 364, 590, 631, 967
Hpy188III TCNNGA 6 cut(s) 33, 35, 260, 523, 537, 939
Hpy8I GTNNAC 1 cut(s) 437
Hpy99I CGWCG 2 cut(s) 934, 1023
HpyAV CCTTC 4 cut(s) 9, 114, 202, 876
HpyCH4III ACNGT 4 cut(s) 23, 257, 545, 781
HpyCH4IV ACGT 2 cut(s) 557, 812
HpyCH4V TGCA 9 cut(s) 66, 116, 179, 273, 385, 395, 530, 561, 599
HpyF10VI GCNNNNNNNGC 5 cut(s) 471, 567, 601, 1000, 1009
HpyF3I CTNAG 7 cut(s) 6, 339, 399, 587, 698, 964, 976
HpySE526I ACGT 2 cut(s) 557, 812
Hsp92II CATG 6 cut(s) 66, 229, 421, 526, 599, 1050
HspAI GCGC 1 cut(s) 1001
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 3 cut(s) 262, 583, 691
LmnI GCTCC 5 cut(s) 74, 505, 607, 670, 831
Lsp1109I GCAGC 5 cut(s) 78, 330, 756, 846, 913
LweI GCATC 2 cut(s) 59, 294
MaeI CTAG 3 cut(s) 791, 861, 987
MaeII ACGT 2 cut(s) 557, 812
MaeIII GTNAC 1 cut(s) 230
MalI GATC 3 cut(s) 264, 585, 693
MboI GATC 3 cut(s) 262, 583, 691
MboII GAAGA 8 cut(s) 149, 299, 326, 329, 416, 705, 778, 927
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 5 cut(s) 152, 467, 612, 704, 868
MluCI AATT 3 cut(s) 99, 372, 870
MlyI GAGTC 1 cut(s) 953
MroXI GAANNNNTTC 2 cut(s) 321, 544
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 1 cut(s) 1009
MspR9I CCNGG 3 cut(s) 145, 443, 658
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 3 cut(s) 145, 443, 658
MvnI CGCG 1 cut(s) 1003
MwoI GCNNNNNNNGC 5 cut(s) 471, 567, 601, 1000, 1009
NdeI CATATG 1 cut(s) 191
NdeII GATC 3 cut(s) 262, 583, 691
NlaIII CATG 6 cut(s) 66, 229, 421, 526, 599, 1050
NlaIV GGNNCC 6 cut(s) 20, 149, 215, 507, 574, 820
NmuCI GTSAC 1 cut(s) 230
NspI RCATGY 2 cut(s) 229, 599
PaeI GCATGC 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 33
PagI TCATGA 1 cut(s) 522
PciI ACATGT 1 cut(s) 225
PctI GAATGC 1 cut(s) 532
PdmI GAANNNNTTC 2 cut(s) 321, 544
PfeI GAWTC 3 cut(s) 365, 421, 798
PflMI CCANNNNNTGG 1 cut(s) 900
PfoI TCCNGGA 1 cut(s) 656
PkrI GCNGC 7 cut(s) 68, 218, 309, 345, 746, 836, 928
PleI GAGTC 1 cut(s) 953
PpsI GAGTC 1 cut(s) 953
PscI ACATGT 1 cut(s) 225
PshAI GACNNNNGTC 1 cut(s) 811
Psp6I CCWGG 3 cut(s) 143, 441, 656
PspGI CCWGG 3 cut(s) 143, 441, 656
PspN4I GGNNCC 6 cut(s) 20, 149, 215, 507, 574, 820
PspPI GGNCC 2 cut(s) 19, 819
PstI CTGCAG 1 cut(s) 118
PstNI CAGNNNCTG 2 cut(s) 113, 635
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SatI GCNGC 7 cut(s) 67, 217, 308, 344, 745, 835, 927
Sau3AI GATC 3 cut(s) 262, 583, 691
Sau96I GGNCC 2 cut(s) 19, 819
SchI GAGTC 1 cut(s) 953
ScrFI CCNGG 3 cut(s) 145, 443, 658
SduI GDGCHC 5 cut(s) 152, 467, 612, 704, 868
SfaNI GCATC 2 cut(s) 59, 294
SfcI CTRYAG 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 33
SinI GGWCC 2 cut(s) 19, 819
SlaI CTCGAG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 33
SmoI CTYRAG 1 cut(s) 33
SphI GCATGC 1 cut(s) 599
Sse9I AATT 3 cut(s) 99, 372, 870
SsiI CCGC 6 cut(s) 217, 244, 308, 410, 570, 614
SspMI CTAG 3 cut(s) 791, 861, 987
StyD4I CCNGG 3 cut(s) 143, 441, 656
TaaI ACNGT 4 cut(s) 23, 257, 545, 781
TaiI ACGT 2 cut(s) 560, 815
TaqI TCGA 2 cut(s) 34, 827
TasI AATT 3 cut(s) 99, 372, 870
TauI GCSGC 2 cut(s) 219, 310
TfiI GAWTC 3 cut(s) 365, 421, 798
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 5 cut(s) 66, 343, 744, 834, 926
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 186, 511, 539
TspGWI ACGGA 1 cut(s) 1036
Van91I CCANNNNNTGG 1 cut(s) 900
VpaK11BI GGWCC 2 cut(s) 19, 819
XapI RAATTY 1 cut(s) 99
XceI RCATGY 2 cut(s) 229, 599
XhoI CTCGAG 1 cut(s) 33
XmnI GAANNNNTTC 2 cut(s) 321, 544
XspI CTAG 3 cut(s) 791, 861, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.