AT3G27180
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013116
Physical Location & Seq
Reverse (-)
10026837 .. 10030087
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27180.1

Sequence Viewer

Length: 1557 bp
ATGATGAATTCGTCGAGCCTTTTCTCGTTCGGCGGTCGTTTTCCAATCATCAACTGCCGCGTGTCAGCTCCTGCTTCGGCTCCCTTCGTCGCTTCCGTTCCTCTCCCGCCGTCAACCTCCTCCGACTCACTAGCTTGCTCTCTCCAATGTCCTCATTTCCAATCGTGCTCAGGATGTACGCAAGAGTTCAATCTCCATCGTCCAGCTGTTGTCGACGAAGCTTCAGACTTCTTCAAGCGTTACGGCGTCGATGATTTCACTTTCGACAGTTGTAGACTGTGGGGATGGAGATGCCGAGCAAAATTGGCTGTTCGTGGCTCTTCAGATAACGCATTAATTGGATTGTACCAAGAAGGAACTCACACTGTTGTTGATATTCCTGAATGCAAATCTCATCATCCTAATATTAATGCTGCCATTGAGTTATTGAGAGAAGGTATTAAGGTGTTTGATGTTGTGCCATTTGATGAGGATCAGGGTACTGGGGACTTACGATATGTTCAGATGGCTGTTACGACGCATAGTACAAATCTTCGTGCTCCAGAGAGATATAAAAATGGAAAAGTGCAGGTGTCCTTGGTTTGGAACTCGAGAAATGAGAGATCTCATAATGCAGATAAGTTGCAAGCATTGTCCACCTACTTGTGGAGAAAGGGTGGACCAAACAGCAAGTTTCACCTGATACATTCTGTCTGGGCTAACTTTCAGACATCAACCAACAATATAATCTTTGGAAACAGATGGAGGCATCTTTTAGGTGAGAGAGATTTCTGGGAACATGTAGGAGGAATTGACATATCCTTGGATCCTTCCAGTTTTGGCCAGGCAAATACTCGGGCTTTTGATAGCTTGCTTTGGAAACTGCATAAGTATGTTCCTGGTGGATCATCTGTTGCTGATCTATATGCAGGAGCCGGAGTGATTGGATTATCGTTAGCTACATCGAGGAAATGCAGTTCTGTCAAATGCATTGAGGTTAACAAAGAAGCAAGGCTGTCTTTTGAGAAAACAATCCAGCGGTTGCCGAACTCATTGAATTGCAGCATCAGTTGGCACCATGCAGATGCTTCAGTTAACCCTCTTTCGTGGATAATAGGATCAGATGTAGTCGTTGTGGATCCTCCTAGGAGAGGTCTGGATGCTTCTCTCCGTCAGATGCTGGAGTCTGTTCCCTCCATTGAGAAAAGAATGAGGTCGTCATCACAGAGTTCAAGCTTGAACGCAAAAGAGGAGAAGAGGCCGTGGATTTTAAGAGCAAAGGAACTTTCAATTCAAGCTGGCAACAAGCAGACCTCTGAGGAGAACAATACACTACCGCAAAGACTCATATATATAAGCTGTGGTTGGGAAAGCTTTAAAGAGGACTGCAAGTCGTTATTATCTAGCAGAGCGTGGGAGTTGGAAAAAGCCCATGGCTTCAACTTCTTTCCCGGAACCGACAGCATTGAAGTTTTGGCCGTATTCAAAAGAAGGGTTGCGAGCAAGAAAAAGAAGAAATCGGGAATAAAAAAAGTGGGAATTAAGAAAGTGCGTGCTAAGTCACAGGACTCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

518

Amino Acids

57.84

Weight (kDa)

9.31

Isoelectric Point (pI)

50.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cons_hypoth95 PF03602 295 - 380 1.5e-06 Conserved hypothetical protein 95
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 559
Acc36I ACCTGC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 1053
AccI GTMKAC 2 cut(s) 213, 274
AccII CGCG 1 cut(s) 60
AciI CCGC 5 cut(s) 33, 58, 107, 1018, 1316
AclWI GGATC 7 cut(s) 480, 800, 813, 892, 1105, 1112, 1125
AcoI YGGCCR 2 cut(s) 820, 1455
AcsI RAATTY 1 cut(s) 7
AcuI CTGAAG 3 cut(s) 207, 306, 1053
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 4 cut(s) 178, 347, 481, 526
AfiI CCNNNNNNNGG 3 cut(s) 582, 645, 1130
AflIII ACRYGT 1 cut(s) 778
AhdI GACNNNNNGTC 1 cut(s) 1369
AjnI CCWGG 2 cut(s) 822, 877
AloI GAACNNNNNNTCC 2 cut(s) 940, 972
Alw21I GWGCWC 2 cut(s) 170, 541
AlwI GGATC 7 cut(s) 480, 800, 813, 892, 1105, 1112, 1125
AlwNI CAGNNNCTG 2 cut(s) 71, 1159
Ama87I CYCGRG 2 cut(s) 589, 834
AoxI GGCC 3 cut(s) 820, 1238, 1455
ApeKI GCWGC 2 cut(s) 413, 1041
ApoI RAATTY 1 cut(s) 7
AseI ATTAAT 2 cut(s) 335, 408
AspA2I CCTAGG 1 cut(s) 1124
AspS9I GGNCC 1 cut(s) 659
AsuC2I CCSGG 1 cut(s) 1431
AsuHPI GGTGA 2 cut(s) 668, 770
AvaI CYCGRG 2 cut(s) 589, 834
AvaII GGWCC 1 cut(s) 659
AvrII CCTAGG 1 cut(s) 1124
BalI TGGCCA 1 cut(s) 822
BamHI GGATCC 2 cut(s) 805, 1117
BanI GGYRCC 1 cut(s) 1053
Bbv12I GWGCWC 2 cut(s) 170, 541
BbvI GCAGC 2 cut(s) 400, 1053
BccI CCATC 4 cut(s) 204, 279, 499, 735
BceAI ACGGC 4 cut(s) 94, 259, 1225, 1442
BciT130I CCWGG 2 cut(s) 824, 879
BcnI CCSGG 1 cut(s) 1431
BfaI CTAG 3 cut(s) 131, 1125, 1383
BfuAI ACCTGC 1 cut(s) 559
BglII AGATCT 1 cut(s) 602
BisI GCNGC 3 cut(s) 58, 414, 1042
BlnI CCTAGG 1 cut(s) 1124
BlsI GCNGC 3 cut(s) 59, 415, 1043
Bme1390I CCNGG 3 cut(s) 824, 879, 1431
Bme18I GGWCC 1 cut(s) 659
BmeRI GACNNNNNGTC 1 cut(s) 1369
BmeT110I CYCGRG 2 cut(s) 589, 834
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 6 cut(s) 81, 807, 913, 1055, 1119, 1435
BmrFI CCNGG 3 cut(s) 824, 879, 1431
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 6 cut(s) 281, 757, 1053, 1054, 1129, 1146
BmuI ACTGGG 1 cut(s) 492
BpmI CTGGAG 2 cut(s) 525, 1181
Bpu10I CCTNAGC 1 cut(s) 169
BpuMI CCSGG 1 cut(s) 1431
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 5 cut(s) 576, 801, 1124, 1241, 1411
BsaXI ACNNNNNCTCC 4 cut(s) 640, 670, 903, 933
Bsc4I CCNNNNNNNGG 3 cut(s) 582, 645, 1130
Bse1I ACTGG 2 cut(s) 487, 813
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 2 cut(s) 824, 879
BseDI CCNNGG 5 cut(s) 576, 801, 1124, 1241, 1411
BseGI GGATG 4 cut(s) 179, 290, 397, 1144
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 3 cut(s) 582, 645, 1130
BseMII CTCAG 2 cut(s) 183, 1287
BseNI ACTGG 2 cut(s) 487, 813
BseRI GAGGAG 3 cut(s) 109, 1244, 1313
BseXI GCAGC 2 cut(s) 400, 1053
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 3 cut(s) 822, 1240, 1457
BshNI GGYRCC 1 cut(s) 1053
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 2 cut(s) 170, 541
BsiHKCI CYCGRG 2 cut(s) 589, 834
BsiSI CCGG 2 cut(s) 915, 1431
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 3 cut(s) 582, 645, 1130
BsmFI GGGAC 1 cut(s) 500
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 3 cut(s) 822, 1240, 1457
BsoBI CYCGRG 2 cut(s) 589, 834
Bsp1286I GDGCHC 2 cut(s) 170, 541
Bsp143I GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
Bsp19I CCATGG 1 cut(s) 1411
BspACI CCGC 5 cut(s) 33, 58, 107, 1018, 1316
BspANI GGCC 3 cut(s) 822, 1240, 1457
BspCNI CTCAG 2 cut(s) 182, 1288
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 6 cut(s) 81, 807, 913, 1055, 1119, 1435
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 7 cut(s) 480, 800, 813, 892, 1105, 1112, 1125
BspQI GCTCTTC 1 cut(s) 325
BspT107I GGYRCC 1 cut(s) 1053
BsrI ACTGG 2 cut(s) 487, 813
BssECI CCNNGG 5 cut(s) 576, 801, 1124, 1241, 1411
BssMI GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 4 cut(s) 576, 801, 1124, 1411
Bst2UI CCWGG 2 cut(s) 824, 879
Bst4CI ACNGT 3 cut(s) 269, 279, 367
Bst6I CTCTTC 2 cut(s) 325, 1229
BstACI GRCGYC 1 cut(s) 246
BstC8I GCNNGC 6 cut(s) 136, 627, 851, 1279, 1480, 1533
BstDEI CTNAG 3 cut(s) 169, 1296, 1536
BstDSI CCRYGG 2 cut(s) 1241, 1411
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 4 cut(s) 179, 290, 397, 1144
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 7 cut(s) 475, 605, 808, 887, 901, 1100, 1120
BstMBI GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 2 cut(s) 824, 879
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 3 cut(s) 822, 877, 1429
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 400, 1053
BstX2I RGATCY 3 cut(s) 602, 805, 1117
BstYI RGATCY 3 cut(s) 602, 805, 1117
BsuRI GGCC 3 cut(s) 822, 1240, 1457
BtgI CCRYGG 2 cut(s) 1241, 1411
BtsCI GGATG 4 cut(s) 179, 290, 397, 1144
BtsIMutI CAGTG 1 cut(s) 363
BveI ACCTGC 1 cut(s) 559
Cac8I GCNNGC 6 cut(s) 136, 627, 851, 1279, 1480, 1533
CaiI CAGNNNCTG 2 cut(s) 71, 1159
Cfr13I GGNCC 1 cut(s) 659
CseI GACGC 2 cut(s) 235, 526
Csp6I GTAC 4 cut(s) 177, 346, 480, 525
CviAII CATG 3 cut(s) 779, 1058, 1412
CviQI GTAC 4 cut(s) 177, 346, 480, 525
DdeI CTNAG 3 cut(s) 169, 1296, 1536
DpnI GATC 7 cut(s) 474, 604, 807, 886, 900, 1099, 1119
DpnII GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
DraI TTTAAA 1 cut(s) 1357
DriI GACNNNNNGTC 1 cut(s) 1369
EaeI YGGCCR 2 cut(s) 820, 1455
Eam1104I CTCTTC 2 cut(s) 325, 1229
Eam1105I GACNNNNNGTC 1 cut(s) 1369
EarI CTCTTC 2 cut(s) 325, 1229
Eco130I CCWWGG 4 cut(s) 576, 801, 1124, 1411
Eco47I GGWCC 1 cut(s) 659
Eco57I CTGAAG 3 cut(s) 207, 306, 1053
Eco88I CYCGRG 2 cut(s) 589, 834
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 822, 877
EcoT14I CCWWGG 4 cut(s) 576, 801, 1124, 1411
EcoT22I ATGCAT 1 cut(s) 971
ErhI CCWWGG 4 cut(s) 576, 801, 1124, 1411
FaeI CATG 3 cut(s) 782, 1061, 1415
FalI AAGNNNNNCTT 2 cut(s) 982, 1014
FaqI GGGAC 1 cut(s) 500
FatI CATG 3 cut(s) 778, 1057, 1411
FauI CCCGC 1 cut(s) 114
FblI GTMKAC 2 cut(s) 213, 274
Fnu4HI GCNGC 3 cut(s) 58, 414, 1042
FokI GGATG 4 cut(s) 186, 297, 384, 1151
Fsp4HI GCNGC 3 cut(s) 58, 414, 1042
FspBI CTAG 3 cut(s) 131, 1125, 1383
GluI GCNGC 3 cut(s) 58, 414, 1042
GsuI CTGGAG 2 cut(s) 525, 1181
HaeIII GGCC 3 cut(s) 822, 1240, 1457
HapII CCGG 2 cut(s) 915, 1431
HgaI GACGC 2 cut(s) 235, 526
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 3 cut(s) 782, 1061, 1415
HincII GTYRAC 4 cut(s) 114, 214, 979, 1075
HindII GTYRAC 4 cut(s) 114, 214, 979, 1075
HindIII AAGCTT 3 cut(s) 219, 1213, 1351
HinfI GANTC 4 cut(s) 125, 1163, 1323, 1547
HpaI GTTAAC 2 cut(s) 979, 1075
HpaII CCGG 2 cut(s) 915, 1431
HphI GGTGA 2 cut(s) 668, 770
Hpy166II GTNNAC 7 cut(s) 114, 214, 275, 636, 659, 979, 1075
Hpy188I TCNGA 8 cut(s) 124, 226, 325, 504, 708, 1102, 1155, 1297
Hpy188III TCNNGA 6 cut(s) 171, 380, 542, 591, 1136, 1500
Hpy8I GTNNAC 7 cut(s) 114, 214, 275, 636, 659, 979, 1075
Hpy99I CGWCG 5 cut(s) 16, 92, 218, 251, 520
HpyAV CCTTC 5 cut(s) 94, 347, 428, 819, 1464
HpyCH4III ACNGT 3 cut(s) 269, 279, 367
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 169, 1296, 1536
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 3 cut(s) 782, 1061, 1415
KspAI GTTAAC 2 cut(s) 979, 1075
Kzo9I GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
LguI GCTCTTC 1 cut(s) 325
LmnI GCTCC 4 cut(s) 73, 85, 544, 911
Lsp1109I GCAGC 2 cut(s) 400, 1053
LweI GCATC 6 cut(s) 281, 757, 1053, 1054, 1129, 1146
MaeI CTAG 3 cut(s) 131, 1125, 1383
MaeIII GTNAC 3 cut(s) 239, 511, 1539
MalI GATC 7 cut(s) 474, 604, 807, 886, 900, 1099, 1119
MboI GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
MboII GAAGA 5 cut(s) 223, 312, 524, 1246, 1504
MflI RGATCY 3 cut(s) 602, 805, 1117
MhlI GDGCHC 2 cut(s) 170, 541
MlsI TGGCCA 1 cut(s) 822
MluCI AATT 7 cut(s) 7, 302, 336, 789, 1036, 1269, 1518
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 4 cut(s) 119, 1172, 1317, 1541
MmeI TCCRAC 2 cut(s) 147, 1380
Mox20I TGGCCA 1 cut(s) 822
Mph1103I ATGCAT 1 cut(s) 971
MscI TGGCCA 1 cut(s) 822
MseI TTAA 8 cut(s) 335, 408, 441, 978, 1074, 1250, 1356, 1521
MslI CAYNNNNRTG 2 cut(s) 870, 1062
Msp20I TGGCCA 1 cut(s) 822
MspA1I CMGCKG 2 cut(s) 206, 1018
MspI CCGG 2 cut(s) 915, 1431
MspR9I CCNGG 3 cut(s) 824, 879, 1431
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 2 cut(s) 824, 879
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 1431
NcoI CCATGG 1 cut(s) 1411
NdeII GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
NlaIII CATG 3 cut(s) 782, 1061, 1415
NlaIV GGNNCC 6 cut(s) 81, 807, 913, 1055, 1119, 1435
NmeAIII GCCGAG 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 1539
NsiI ATGCAT 1 cut(s) 971
NspI RCATGY 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 589
PaqCI CACCTGC 1 cut(s) 559
PciI ACATGT 1 cut(s) 778
PciSI GCTCTTC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 1 cut(s) 389
PfoI TCCNGGA 1 cut(s) 1429
PkrI GCNGC 3 cut(s) 59, 415, 1043
PleI GAGTC 4 cut(s) 119, 1171, 1317, 1541
PpsI GAGTC 4 cut(s) 119, 1171, 1317, 1541
PscI ACATGT 1 cut(s) 778
PshBI ATTAAT 2 cut(s) 335, 408
Psp6I CCWGG 2 cut(s) 822, 877
PspGI CCWGG 2 cut(s) 822, 877
PspN4I GGNNCC 6 cut(s) 81, 807, 913, 1055, 1119, 1435
PspPI GGNCC 1 cut(s) 659
PstNI CAGNNNCTG 2 cut(s) 71, 1159
PsuI RGATCY 3 cut(s) 602, 805, 1117
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 4 cut(s) 178, 347, 481, 526
RsaNI GTAC 4 cut(s) 177, 346, 480, 525
RseI CAYNNNNRTG 2 cut(s) 870, 1062
SalI GTCGAC 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 325
SaqAI TTAA 8 cut(s) 335, 408, 441, 978, 1074, 1250, 1356, 1521
SatI GCNGC 3 cut(s) 58, 414, 1042
Sau3AI GATC 7 cut(s) 472, 602, 805, 884, 898, 1097, 1117
Sau96I GGNCC 1 cut(s) 659
SchI GAGTC 4 cut(s) 119, 1172, 1317, 1541
ScrFI CCNGG 3 cut(s) 824, 879, 1431
SduI GDGCHC 2 cut(s) 170, 541
SfaNI GCATC 6 cut(s) 281, 757, 1053, 1054, 1129, 1146
Sfr274I CTCGAG 1 cut(s) 589
SinI GGWCC 1 cut(s) 659
SlaI CTCGAG 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 870, 1062
SmlI CTYRAG 1 cut(s) 589
SmoI CTYRAG 1 cut(s) 589
Sse9I AATT 7 cut(s) 7, 302, 336, 789, 1036, 1269, 1518
SsiI CCGC 5 cut(s) 33, 58, 107, 1018, 1316
SspI AATATT 1 cut(s) 406
SspMI CTAG 3 cut(s) 131, 1125, 1383
StyD4I CCNGG 3 cut(s) 822, 877, 1429
StyI CCWWGG 4 cut(s) 576, 801, 1124, 1411
TaaI ACNGT 3 cut(s) 269, 279, 367
TaqI TCGA 6 cut(s) 14, 213, 249, 264, 590, 944
TasI AATT 7 cut(s) 7, 302, 336, 789, 1036, 1269, 1518
TatI WGTACW 1 cut(s) 524
TauI GCSGC 1 cut(s) 60
Tru1I TTAA 8 cut(s) 335, 408, 441, 978, 1074, 1250, 1356, 1521
Tru9I TTAA 8 cut(s) 335, 408, 441, 978, 1074, 1250, 1356, 1521
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 1 cut(s) 1539
TseI GCWGC 2 cut(s) 413, 1041
Tsp45I GTSAC 1 cut(s) 1539
TspDTI ATGAA 1 cut(s) 20
TspGWI ACGGA 2 cut(s) 85, 1139
TspRI CASTG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 659
VspI ATTAAT 2 cut(s) 335, 408
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 1 cut(s) 7
XceI RCATGY 1 cut(s) 782
XhoI CTCGAG 1 cut(s) 589
XmaJI CCTAGG 1 cut(s) 1124
XmiI GTMKAC 2 cut(s) 213, 274
XspI CTAG 3 cut(s) 131, 1125, 1383
Zsp2I ATGCAT 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.