Rorug02G0583500
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013116
Physical Location & Seq
Forward (+)
70661472 .. 70662564
1093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0583500.1

Sequence Viewer

Length: 459 bp
ATGGCCGACGAGGAAGAAAAGACGGCGTCGTTTAAGAGTTTGGGTCTATGCGATGAACTGGTGAATGCTGTTGGTAAACTGGAATGGACGTCCCCTACCCCTATTCAAGTGGAGACCATTCCTCACGCCCTCGCAGGCAGGAAAAGACTTGATCGCACTTGCCCAAACGGGTTCAGGGAAAACCTGAGCAAAGCCTTTTTTGCTTTGGTTCTCTCCCCAACAAGGGAGCTTGCCGTTCAGATCAAAGACCACTTTGACGCCCTCGGGAAAGACATATTGGTGCCAAGTCTGCTGCGCTCAATAACTAGCTTTTGTTTGCTTTTGTCTCTAATCATCGGTTCTGCTCAGCTTATTGGAGGACTGAACATGGCGCAAGAATCTAGGGTTCTTGCAACGCGGCCACACGTCATTGTTGCGACGTCCGGACGCCTCCTACATCATCTGCGTGACACGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

152

Amino Acids

16.67

Weight (kDa)

8.54

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 63 - 149 2.7e-07 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 92, 422
AccB1I GGYRCC 1 cut(s) 280
AccII CGCG 1 cut(s) 397
AccIII TCCGGA 1 cut(s) 422
AciI CCGC 1 cut(s) 397
AcoI YGGCCR 2 cut(s) 3, 398
AcyI GRCGYC 5 cut(s) 26, 89, 258, 419, 427
AfiI CCNNNNNNNGG 2 cut(s) 222, 223
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 1 cut(s) 107
AjiI CACGTC 1 cut(s) 406
AjuI GAANNNNNNNTTGG 2 cut(s) 260, 292
AluBI AGCT 3 cut(s) 229, 309, 349
AluI AGCT 3 cut(s) 229, 309, 349
Alw26I GTCTC 2 cut(s) 107, 330
Ama87I CYCGRG 1 cut(s) 263
Aor13HI TCCGGA 1 cut(s) 422
AoxI GGCC 2 cut(s) 3, 398
ApeKI GCWGC 1 cut(s) 292
AspLEI GCGC 2 cut(s) 297, 373
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 263
BanI GGYRCC 1 cut(s) 280
BbvI GCAGC 1 cut(s) 279
BceAI ACGGC 2 cut(s) 39, 218
BcoDI GTCTC 2 cut(s) 107, 330
BfaI CTAG 2 cut(s) 306, 381
BisI GCNGC 2 cut(s) 293, 398
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 2 cut(s) 294, 399
BmeT110I CYCGRG 1 cut(s) 263
BmgBI CACGTC 1 cut(s) 406
BmiI GGNNCC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 185
Bpu1102I GCTNAGC 1 cut(s) 345
BsaHI GRCGYC 5 cut(s) 26, 89, 258, 419, 427
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 262
BsaWI WCCGGW 1 cut(s) 422
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 223
Bse1I ACTGG 2 cut(s) 63, 84
BseAI TCCGGA 1 cut(s) 422
BseDI CCNNGG 1 cut(s) 262
BseLI CCNNNNNNNGG 2 cut(s) 222, 223
BseMII CTCAG 2 cut(s) 176, 359
BseNI ACTGG 2 cut(s) 63, 84
BseXI GCAGC 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 5, 400
BshNI GGYRCC 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 423
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 2 cut(s) 222, 223
BsmAI GTCTC 2 cut(s) 107, 330
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 2 cut(s) 5, 400
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 263
Bsp13I TCCGGA 1 cut(s) 422
Bsp143I GATC 2 cut(s) 151, 240
Bsp1720I GCTNAGC 1 cut(s) 345
BspACI CCGC 1 cut(s) 397
BspANI GGCC 2 cut(s) 5, 400
BspCNI CTCAG 2 cut(s) 177, 358
BspEI TCCGGA 1 cut(s) 422
BspFNI CGCG 1 cut(s) 397
BspLI GGNNCC 1 cut(s) 282
BspT107I GGYRCC 1 cut(s) 280
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 2 cut(s) 63, 84
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 2 cut(s) 151, 240
BssNI GRCGYC 5 cut(s) 26, 89, 258, 419, 427
BstACI GRCGYC 5 cut(s) 26, 89, 258, 419, 427
BstC8I GCNNGC 2 cut(s) 136, 231
BstDEI CTNAG 2 cut(s) 185, 345
BstFNI CGCG 1 cut(s) 397
BstHHI GCGC 2 cut(s) 297, 373
BstKTI GATC 2 cut(s) 154, 243
BstMAI GTCTC 2 cut(s) 107, 330
BstMBI GATC 2 cut(s) 151, 240
BstMWI GCNNNNNNNGC 2 cut(s) 200, 289
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 1 cut(s) 279
BsuRI GGCC 2 cut(s) 5, 400
BtgZI GCGATG 1 cut(s) 66
BtrI CACGTC 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 136, 231
CfoI GCGC 2 cut(s) 297, 373
CseI GACGC 3 cut(s) 15, 266, 435
CviAII CATG 1 cut(s) 367
CviJI RGCY 6 cut(s) 5, 194, 229, 309, 349, 400
CviKI_1 RGCY 6 cut(s) 5, 194, 229, 309, 349, 400
DdeI CTNAG 2 cut(s) 185, 345
DpnI GATC 2 cut(s) 153, 242
DpnII GATC 2 cut(s) 151, 240
EaeI YGGCCR 2 cut(s) 3, 398
Eco31I GGTCTC 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 263
FaeI CATG 1 cut(s) 370
FaiI YATR 3 cut(s) 49, 275, 368
FaqI GGGAC 1 cut(s) 76
FatI CATG 1 cut(s) 366
Fnu4HI GCNGC 2 cut(s) 293, 398
Fsp4HI GCNGC 2 cut(s) 293, 398
FspBI CTAG 2 cut(s) 306, 381
GlaI GCGC 2 cut(s) 296, 372
GluI GCNGC 2 cut(s) 293, 398
HaeIII GGCC 2 cut(s) 5, 400
HapII CCGG 1 cut(s) 423
HgaI GACGC 3 cut(s) 15, 266, 435
HhaI GCGC 2 cut(s) 297, 373
Hin1I GRCGYC 5 cut(s) 26, 89, 258, 419, 427
Hin1II CATG 1 cut(s) 370
Hin6I GCGC 2 cut(s) 295, 371
HinP1I GCGC 2 cut(s) 295, 371
HinfI GANTC 1 cut(s) 377
HpaII CCGG 1 cut(s) 423
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 77
Hpy188I TCNGA 1 cut(s) 240
Hpy188III TCNNGA 2 cut(s) 265, 423
Hpy8I GTNNAC 1 cut(s) 77
Hpy99I CGWCG 3 cut(s) 11, 31, 421
HpyCH4IV ACGT 3 cut(s) 89, 405, 419
HpyCH4V TGCA 1 cut(s) 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 289
HpyF3I CTNAG 2 cut(s) 185, 345
HpySE526I ACGT 3 cut(s) 89, 405, 419
Hsp92I GRCGYC 5 cut(s) 26, 89, 258, 419, 427
Hsp92II CATG 1 cut(s) 370
HspAI GCGC 2 cut(s) 295, 371
Kpn2I TCCGGA 1 cut(s) 422
Kzo9I GATC 2 cut(s) 151, 240
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 7 cut(s) 44, 65, 120, 124, 160, 197, 436
Lsp1109I GCAGC 1 cut(s) 279
MaeI CTAG 2 cut(s) 306, 381
MaeII ACGT 3 cut(s) 89, 405, 419
MaeIII GTNAC 1 cut(s) 446
MalI GATC 2 cut(s) 153, 242
MboI GATC 2 cut(s) 151, 240
MboII GAAGA 1 cut(s) 26
MnlI CCTC 6 cut(s) 4, 132, 140, 272, 350, 440
MroI TCCGGA 1 cut(s) 422
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 2 cut(s) 278, 444
MspI CCGG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 70
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 2 cut(s) 200, 289
NdeII GATC 2 cut(s) 151, 240
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 446
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 377
PflFI GACNNNGTC 1 cut(s) 25
PkrI GCNGC 2 cut(s) 294, 399
PspN4I GGNNCC 1 cut(s) 282
PsyI GACNNNGTC 1 cut(s) 25
RseI CAYNNNNRTG 2 cut(s) 278, 444
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 2 cut(s) 293, 398
Sau3AI GATC 2 cut(s) 151, 240
SetI ASST 7 cut(s) 92, 186, 231, 311, 351, 408, 422
SmiMI CAYNNNNRTG 2 cut(s) 278, 444
SsiI CCGC 1 cut(s) 397
SspMI CTAG 2 cut(s) 306, 381
TaiI ACGT 3 cut(s) 92, 408, 422
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 25
XspI CTAG 2 cut(s) 306, 381
ZraI GACGTC 2 cut(s) 90, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.