Rorug02G0583600
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013116
Physical Location & Seq
Forward (+)
70671265 .. 70672807
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0583600.1

Sequence Viewer

Length: 465 bp
ATGGTTTTTACTGAACAATGCGCCACAACACCCTTTTTGGCTTATACGCTTCAAAATCTTGGAATCAGGGCCATCCCTCTTCATGGTGATATGAGCCAGCAAAAAAGACTTGGAGCCTTAAACATGTTCAAATCTCAACAGTATGATGTACTTGTCAGCACTGATGTGGCAAGTAGAGGATTGGATATTCCGGTAACAGTAGGAAGAACTGCTCGAGCTGGAGAATCCGGGGTTGCAATCACTTTCTTGAGTAGTATACTTGACTTACCAGGGTATTTCCAGATAGAATGGCTTATTGGCAATAAGTCACCTTTATATCCTGATCCAGCCAAAGAAGCTTTACCATTTTTGGAAAGTGTCAGAGAGGCTAAAAGATTGGCCACCTCGGTAATGTTCAGCCTAACGACAAGCGTGGCCCGTGGCCCACCATTGTACCGATACTGTCCCAACTTAACCACCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

154

Amino Acids

16.96

Weight (kDa)

9.0

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 2 - 64 3.1e-13 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 256
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 378
AfaI GTAC 2 cut(s) 150, 434
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 2 cut(s) 53, 130
AjnI CCWGG 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 2 cut(s) 218, 338
AluI AGCT 2 cut(s) 218, 338
AlwI GGATC 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 4 cut(s) 69, 378, 414, 421
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 3 cut(s) 69, 415, 422
AsuC2I CCSGG 1 cut(s) 229
AsuHPI GGTGA 2 cut(s) 98, 300
AvaI CYCGRG 1 cut(s) 213
BaeI ACNNNNGTAYC 2 cut(s) 416, 449
BalI TGGCCA 1 cut(s) 380
BccI CCATC 1 cut(s) 80
BciT130I CCWGG 1 cut(s) 270
BcnI CCSGG 1 cut(s) 229
Bme1390I CCNGG 2 cut(s) 229, 270
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 3 cut(s) 69, 415, 422
BmiI GGNNCC 1 cut(s) 115
BmrFI CCNGG 2 cut(s) 229, 270
BpmI CTGGAG 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 229
BsaJI CCNNGG 4 cut(s) 228, 269, 384, 418
BsaWI WCCGGW 1 cut(s) 190
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 4 cut(s) 228, 269, 384, 418
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 83
BshFI GGCC 4 cut(s) 71, 380, 416, 423
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 2 cut(s) 191, 228
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 429
BsnI GGCC 4 cut(s) 71, 380, 416, 423
BsoBI CYCGRG 1 cut(s) 213
Bsp143I GATC 1 cut(s) 322
BspANI GGCC 4 cut(s) 71, 380, 416, 423
BspLI GGNNCC 1 cut(s) 115
BspPI GGATC 1 cut(s) 317
BssECI CCNNGG 4 cut(s) 228, 269, 384, 418
BssMI GATC 1 cut(s) 322
BssNAI GTATAC 1 cut(s) 257
Bst1107I GTATAC 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 3 cut(s) 141, 199, 443
Bst6I CTCTTC 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 98
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 1 cut(s) 72
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 1 cut(s) 325
BstMBI GATC 1 cut(s) 322
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 227, 268
BstZ17I GTATAC 1 cut(s) 257
BsuRI GGCC 4 cut(s) 71, 380, 416, 423
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 98
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 69, 415, 422
Csp6I GTAC 2 cut(s) 149, 433
CviAII CATG 2 cut(s) 83, 124
CviQI GTAC 2 cut(s) 149, 433
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
EaeI YGGCCR 1 cut(s) 378
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 268
FaeI CATG 2 cut(s) 86, 127
FaiI YATR 7 cut(s) 45, 84, 92, 125, 144, 257, 316
FaqI GGGAC 1 cut(s) 429
FatI CATG 2 cut(s) 82, 123
FblI GTMKAC 1 cut(s) 256
FokI GGATG 1 cut(s) 59
GlaI GCGC 1 cut(s) 22
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 4 cut(s) 71, 380, 416, 423
HapII CCGG 2 cut(s) 191, 228
HhaI GCGC 1 cut(s) 23
Hin1II CATG 2 cut(s) 86, 127
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 2 cut(s) 63, 224
HpaII CCGG 2 cut(s) 191, 228
HphI GGTGA 2 cut(s) 98, 300
Hpy166II GTNNAC 1 cut(s) 257
Hpy188I TCNGA 1 cut(s) 362
Hpy188III TCNNGA 3 cut(s) 247, 280, 320
Hpy8I GTNNAC 1 cut(s) 257
HpyCH4III ACNGT 3 cut(s) 141, 199, 443
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
Hsp92II CATG 2 cut(s) 86, 127
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 322
LmnI GCTCC 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 193, 306
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 2 cut(s) 71, 216
MlsI TGGCCA 1 cut(s) 380
MluNI TGGCCA 1 cut(s) 380
MnlI CCTC 4 cut(s) 87, 170, 358, 394
Mox20I TGGCCA 1 cut(s) 380
MscI TGGCCA 1 cut(s) 380
MseI TTAA 2 cut(s) 119, 452
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 380
MspI CCGG 2 cut(s) 191, 228
MspR9I CCNGG 2 cut(s) 229, 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 335
NciI CCSGG 1 cut(s) 229
NdeII GATC 1 cut(s) 322
NlaIII CATG 2 cut(s) 86, 127
NlaIV GGNNCC 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 306
NspI RCATGY 1 cut(s) 127
OliI CACNNNNGTG 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 213
PciI ACATGT 1 cut(s) 123
PfeI GAWTC 2 cut(s) 63, 224
PscI ACATGT 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 115
PspPI GGNCC 3 cut(s) 69, 415, 422
PspXI VCTCGAGB 1 cut(s) 213
RsaI GTAC 2 cut(s) 150, 434
RsaNI GTAC 2 cut(s) 149, 433
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 2 cut(s) 119, 452
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 3 cut(s) 69, 415, 422
ScrFI CCNGG 2 cut(s) 229, 270
SetI ASST 4 cut(s) 220, 313, 340, 386
Sfr274I CTCGAG 1 cut(s) 213
SlaI CTCGAG 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 2 cut(s) 213, 247
SmoI CTYRAG 2 cut(s) 213, 247
StyD4I CCNGG 2 cut(s) 227, 268
TaaI ACNGT 3 cut(s) 141, 199, 443
TaqI TCGA 1 cut(s) 214
TatI WGTACW 1 cut(s) 148
TfiI GAWTC 2 cut(s) 63, 224
Tru1I TTAA 2 cut(s) 119, 452
Tru9I TTAA 2 cut(s) 119, 452
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 306
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 71
TspRI CASTG 1 cut(s) 166
XceI RCATGY 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 213
XmiI GTMKAC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.