pycom111g01050
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0013116
Physical Location & Seq
Reverse (-)
670538 .. 672334
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01050.2

Sequence Viewer

Length: 759 bp
ATGACTCCGGGGAAATTTTTCCATCAACATCTGTTTTGTCTATTATCTATTCCCGCCACAGGGCAACGGCCGCCGGACAACATGCCTCTCTGCTTCCTCTCCGTCCGTCCTCCTCCGCCTCCACTCATCACCTGCCACGTGTCCCCAACCGCAGTGCGTCCCCCGCCGTCCGCTTCAGCACCACCACAAGTACCAACAAGACTACCTTCTCTCTCACTGAGCCCTAGCTCAACCCCTCCTCCGCCGCCGCTGACCTGCGCCCTCCACTGCCCGCACTTCGAATCGTGCTCCGGGTGTACGCACGAGTTCAATCTCCACCGTCCGGTCATCGTCGACGAGGCGACGGAGTTTTTCAAGACAGTTGGAGTCTCAGATTTCACTTTTGACAGTTGCAAATTGTGGGGATGGCGGTGTCGCGCAAAGTTAGCCGTTCGTGGCTCACCGGAAACCCCCCTGATTGGGCTTTATCAGGAGGGAACTCATAATGTAGTAGATATTCCTCAATGCAAAGCTCATCATCCAAGTATAAATGCTGCTGTGGAGCTGCTTAAACAAGGTAAAGTGCAGGTAGCTTTAGTATGGAATTCAAGAAATGAAAATTCCCCGAATACAGAGAAACTGCAAGCCTTGGCTAATATAATCTTTGGGAATAGATGGAGACATCTTTTAGGAGAAAGAGACTTTTGGGAACATGTTGGAGGAATTGATATTTCCTTGGCTCCGTCCAGTTTTGGCCAAGCAAACACAAGGGTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

253

Amino Acids

27.72

Weight (kDa)

8.37

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 140
Acc36I ACCTGC 3 cut(s) 140, 263, 556
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 417
AcoI YGGCCR 2 cut(s) 68, 733
AcsI RAATTY 3 cut(s) 14, 583, 598
AcuI CTGAAG 1 cut(s) 159
AcvI CACGTG 1 cut(s) 139
AfaI GTAC 2 cut(s) 192, 298
AfiI CCNNNNNNNGG 5 cut(s) 59, 60, 322, 458, 459
AflIII ACRYGT 2 cut(s) 138, 691
AgsI TTSAA 3 cut(s) 310, 355, 588
AluBI AGCT 4 cut(s) 228, 512, 544, 572
AluI AGCT 4 cut(s) 228, 512, 544, 572
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 3 cut(s) 373, 652, 672
AoxI GGCC 2 cut(s) 68, 733
ApeKI GCWGC 2 cut(s) 533, 544
ApoI RAATTY 3 cut(s) 14, 583, 598
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 260, 419
AsuC2I CCSGG 2 cut(s) 9, 292
AsuHPI GGTGA 2 cut(s) 121, 432
AsuII TTCGAA 1 cut(s) 279
BalI TGGCCA 1 cut(s) 735
BanII GRGCYC 1 cut(s) 224
BauI CACGAG 1 cut(s) 302
BbrPI CACGTG 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 2 cut(s) 520, 531
BccI CCATC 3 cut(s) 30, 399, 648
BceAI ACGGC 3 cut(s) 83, 151, 413
BcnI CCSGG 2 cut(s) 9, 292
BcoDI GTCTC 3 cut(s) 373, 652, 672
BfaI CTAG 1 cut(s) 225
BfuAI ACCTGC 3 cut(s) 140, 263, 556
BisI GCNGC 5 cut(s) 71, 245, 248, 534, 545
BlsI GCNGC 5 cut(s) 72, 246, 249, 535, 546
Bme1390I CCNGG 2 cut(s) 9, 292
BmiI GGNNCC 1 cut(s) 720
BmrFI CCNGG 2 cut(s) 9, 292
Bpu14I TTCGAA 1 cut(s) 279
BpuMI CCSGG 2 cut(s) 9, 292
BsaAI YACGTR 1 cut(s) 139
BsaJI CCNNGG 3 cut(s) 8, 627, 714
BsaWI WCCGGW 2 cut(s) 322, 442
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 60, 322, 458, 459
Bse1I ACTGG 1 cut(s) 726
BseDI CCNNGG 3 cut(s) 8, 627, 714
BseGI GGATG 2 cut(s) 410, 517
BseLI CCNNNNNNNGG 5 cut(s) 59, 60, 322, 458, 459
BseMII CTCAG 2 cut(s) 209, 384
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 2 cut(s) 102, 228
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 520, 531
BsgI GTGCAG 1 cut(s) 584
Bsh1236I CGCG 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 70, 735
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 290
BsiSI CCGG 5 cut(s) 8, 74, 291, 323, 443
BslFI GGGAC 2 cut(s) 127, 144
BslI CCNNNNNNNGG 5 cut(s) 59, 60, 322, 458, 459
BsmAI GTCTC 3 cut(s) 373, 652, 672
BsmFI GGGAC 2 cut(s) 127, 144
BsnI GGCC 2 cut(s) 70, 735
Bsp119I TTCGAA 1 cut(s) 279
Bsp1286I GDGCHC 2 cut(s) 224, 290
BspANI GGCC 2 cut(s) 70, 735
BspCNI CTCAG 2 cut(s) 210, 383
BspFNI CGCG 1 cut(s) 417
BspLI GGNNCC 1 cut(s) 720
BspMI ACCTGC 3 cut(s) 140, 263, 556
BspT104I TTCGAA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 726
BssECI CCNNGG 3 cut(s) 8, 627, 714
BssSI CACGAG 1 cut(s) 302
BssT1I CCWWGG 2 cut(s) 627, 714
Bst2BI CACGAG 1 cut(s) 302
Bst4CI ACNGT 3 cut(s) 320, 361, 389
BstBAI YACGTR 1 cut(s) 139
BstBI TTCGAA 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 272, 624
BstDEI CTNAG 2 cut(s) 218, 370
BstF5I GGATG 2 cut(s) 410, 517
BstFNI CGCG 1 cut(s) 417
BstHHI GCGC 2 cut(s) 260, 419
BstMAI GTCTC 3 cut(s) 373, 652, 672
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 3 cut(s) 70, 163, 425
BstNSI RCATGY 2 cut(s) 85, 695
BstSCI CCNGG 2 cut(s) 7, 290
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 520, 531
BstZI CGGCCG 1 cut(s) 68
BsuRI GGCC 2 cut(s) 70, 735
BtsCI GGATG 2 cut(s) 410, 517
BtsI GCAGTG 2 cut(s) 159, 265
BtsIMutI CAGTG 3 cut(s) 159, 215, 265
BveI ACCTGC 3 cut(s) 140, 263, 556
Cac8I GCNNGC 2 cut(s) 272, 624
CfoI GCGC 2 cut(s) 260, 419
CseI GACGC 1 cut(s) 146
Csp6I GTAC 2 cut(s) 191, 297
CviAII CATG 2 cut(s) 82, 692
CviQI GTAC 2 cut(s) 191, 297
DdeI CTNAG 2 cut(s) 218, 370
EaeI YGGCCR 2 cut(s) 68, 733
EagI CGGCCG 1 cut(s) 68
EciI GGCGGA 2 cut(s) 105, 231
EclXI CGGCCG 1 cut(s) 68
Eco130I CCWWGG 2 cut(s) 627, 714
Eco24I GRGCYC 1 cut(s) 224
Eco52I CGGCCG 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 159
Eco72I CACGTG 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 583
EcoT14I CCWWGG 2 cut(s) 627, 714
EcoT38I GRGCYC 1 cut(s) 224
ErhI CCWWGG 2 cut(s) 627, 714
FaeI CATG 2 cut(s) 85, 695
FaiI YATR 6 cut(s) 83, 483, 527, 580, 638, 693
FalI AAGNNNNNCTT 2 cut(s) 190, 222
FaqI GGGAC 2 cut(s) 127, 144
FatI CATG 2 cut(s) 81, 691
FauI CCCGC 3 cut(s) 61, 171, 279
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 5 cut(s) 71, 245, 248, 534, 545
FokI GGATG 2 cut(s) 417, 504
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 5 cut(s) 71, 245, 248, 534, 545
FspBI CTAG 1 cut(s) 225
GlaI GCGC 2 cut(s) 259, 418
GluI GCNGC 5 cut(s) 71, 245, 248, 534, 545
HaeIII GGCC 2 cut(s) 70, 735
HapII CCGG 5 cut(s) 8, 74, 291, 323, 443
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 260, 419
Hin1II CATG 2 cut(s) 85, 695
Hin6I GCGC 2 cut(s) 258, 417
HinP1I GCGC 2 cut(s) 258, 417
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 3 cut(s) 4, 281, 366
HpaII CCGG 5 cut(s) 8, 74, 291, 323, 443
HphI GGTGA 2 cut(s) 121, 432
Hpy166II GTNNAC 2 cut(s) 297, 334
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 3 cut(s) 355, 470, 588
Hpy8I GTNNAC 2 cut(s) 297, 334
Hpy99I CGWCG 3 cut(s) 335, 338, 346
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 3 cut(s) 320, 361, 389
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 4 cut(s) 393, 507, 565, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 163, 425
HpyF3I CTNAG 2 cut(s) 218, 370
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 2 cut(s) 85, 695
HspAI GCGC 2 cut(s) 258, 417
LmnI GCTCC 3 cut(s) 293, 541, 724
Lsp1109I GCAGC 2 cut(s) 520, 531
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 138
MhlI GDGCHC 2 cut(s) 224, 290
MlsI TGGCCA 1 cut(s) 735
MluCI AATT 5 cut(s) 14, 395, 583, 598, 702
MluNI TGGCCA 1 cut(s) 735
MlyI GAGTC 1 cut(s) 375
MmeI TCCRAC 2 cut(s) 343, 676
Mox20I TGGCCA 1 cut(s) 735
MroXI GAANNNNTTC 1 cut(s) 17
MscI TGGCCA 1 cut(s) 735
MseI TTAA 2 cut(s) 549, 757
Msp20I TGGCCA 1 cut(s) 735
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 5 cut(s) 8, 74, 291, 323, 443
MspR9I CCNGG 2 cut(s) 9, 292
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 3 cut(s) 70, 163, 425
NciI CCSGG 2 cut(s) 9, 292
NlaIII CATG 2 cut(s) 85, 695
NlaIV GGNNCC 1 cut(s) 720
NspI RCATGY 2 cut(s) 85, 695
NspV TTCGAA 1 cut(s) 279
PaqCI CACCTGC 1 cut(s) 140
PciI ACATGT 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 281
PkrI GCNGC 5 cut(s) 72, 246, 249, 535, 546
PleI GAGTC 1 cut(s) 374
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 1 cut(s) 374
Ppu21I YACGTR 1 cut(s) 139
PscI ACATGT 1 cut(s) 691
PspCI CACGTG 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 720
RsaI GTAC 2 cut(s) 192, 298
RsaNI GTAC 2 cut(s) 191, 297
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 2 cut(s) 549, 757
SatI GCNGC 5 cut(s) 71, 245, 248, 534, 545
SchI GAGTC 1 cut(s) 375
ScrFI CCNGG 2 cut(s) 9, 292
SduI GDGCHC 2 cut(s) 224, 290
SfuI TTCGAA 1 cut(s) 279
SgrDI CGTCGACG 1 cut(s) 332
Sse9I AATT 5 cut(s) 14, 395, 583, 598, 702
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 7, 290
StyI CCWWGG 2 cut(s) 627, 714
TaaI ACNGT 3 cut(s) 320, 361, 389
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 279, 333
TasI AATT 5 cut(s) 14, 395, 583, 598, 702
TauI GCSGC 3 cut(s) 73, 247, 250
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 2 cut(s) 549, 757
Tru9I TTAA 2 cut(s) 549, 757
TscAI CASTG 3 cut(s) 159, 222, 272
TseI GCWGC 2 cut(s) 533, 544
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 4 cut(s) 91, 95, 359, 711
TspRI CASTG 3 cut(s) 159, 222, 272
XapI RAATTY 3 cut(s) 14, 583, 598
XceI RCATGY 2 cut(s) 85, 695
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.