RLG00000022323
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013116
Physical Location & Seq
Forward (+)
82593322 .. 82596871
3550 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022323

Sequence Viewer

Length: 1509 bp
ATGCCTCTCTGCTTCCTCTCCCCCCACCTTCCTCCTCCGCCACTCATCACCTGCCGCGTGTCCCAGTCCTCACTCCGCACCCTACCTCCTTCTCCGTCACCCTCACCGTCACCGCCGTCTCTCTCACTCTCACTAAGCCCTAACTCAACCCCACCGCCGTCGCTAACCTGCGCCCTCCACTGCCCACACTTCGAACTGTGCTCCGGCTGTACTCACCAGTTCAATCTACACCGTCCGGCCGTCGTCGACGAGGCTACCGAGTTCTTCAAGTCCCTAGGCCTCTCCGATTTCACATTCGACAGTTGCAAACTGTGGGGATGGCGGTGCCGTGCGAAATTGGCCGTTCGCGGCTCATCGGAGCACCCTCTGATTGGGCTGTATCAGGAGGGCACTCATAATATAGTTGATATTCCTGACTGCAAAGCTCATCATCCGAATATCAACGCCGCGGTAGAGCTGCTTAAGAAAGGGATTACTGCACTAAGGATTGAGCCATATGATGAAGATGAGGGGACTGGTGATTTGCGATATGTGCAGATGGCCGTGACAACATACAGTACATCTCTTCCTGCTGCCGAAAGATATAGAAATGGTAAAGTGCAGGTATCTTTAGTATGGAATTCGAGAAATGAAACCTCCCCAAATTCAGAGAAACTGCAAGCCTTGGCCAATTTTATATGGAGGAATGGCGGGCCAAGGAACAATGTCCATCTGATTCACTCTGTGTGGGCTAACTTTCAGACATCAAGTAACAATATAATCTTTGGGAATAGATGGAGACATCTTTTAGGAGAAAGAGACTTTTGGGAGCATGTCGGAGGAATTGATATTTCATTGGATCCATCCAGTTTTGGCCAAGCAAACACACGGGCATTTGATACCCTGCTCCGGAAGCTACAAAGGTATGTCCCTCTTGGAGCATCTGTTGCTGATTTGTATGCAGGAGCAGGTGTTATCGGATTATCATTGGCTGCTACAAGAAAATGCAGAAGGTGTTCAGAGCAATGTTGTCATAGTTTTATTGGAGATTTCTTCCTTTATGATTATTGTGGAATAGAACCTCTTTCCTGGATCATGGGATCAGATGTACTTGTTGTTGATCCTCCTAGAAAGGGTCTGGATGCATCTCTTATTGATGCATTGCGGAGTATATCTTCTATAAAGGATAAAGCTAGGTCATCATCTGAGAGTTCAAGCTTAAAGGACAAGGATGAGAAGAGACCATGGATGCTACGTGCAAGAGAAGATTCAATTCAGATTGGAAGCAAAATGACTTCAGAGGATAGCAAAGTGTTGCCTAAAACCCTCATTTATATAAGTTGTGGATGGGAAAGCTTCAAAGAGGATTGTGAAGACTTGTTGTCAAGTAAGGGATGGCGCTTGGAAAAAGCCCATGGCTTTAATTTTTTCCCTGGCACACAGAGCATCGAGGTTTTAGCTGTGTTCAAGAGGGGCCAATCAGTAAACCCAAAGAAAAAGAAATCAGGAAAGAAGAAGAAACGCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

503

Amino Acids

55.89

Weight (kDa)

8.89

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 59, 938
Acc36I ACCTGC 4 cut(s) 59, 176, 592, 938
AccB1I GGYRCC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 246
AccII CGCG 3 cut(s) 57, 348, 449
AccIII TCCGGA 1 cut(s) 888
AclWI GGATC 5 cut(s) 833, 846, 1079, 1087, 1094
AcoI YGGCCR 5 cut(s) 237, 339, 540, 666, 853
AcsI RAATTY 2 cut(s) 619, 643
AcuI CTGAAG 1 cut(s) 1260
AdeI CACNNNGTG 1 cut(s) 724
AfaI GTAC 3 cut(s) 211, 559, 1089
AfiI CCNNNNNNNGG 3 cut(s) 371, 888, 1112
AflII CTTAAG 1 cut(s) 461
AgsI TTSAA 6 cut(s) 223, 268, 1194, 1251, 1339, 1447
AhdI GACNNNNNGTC 1 cut(s) 1360
AjnI CCWGG 2 cut(s) 1067, 1411
AluBI AGCT 7 cut(s) 425, 457, 895, 1172, 1197, 1335, 1439
AluI AGCT 7 cut(s) 425, 457, 895, 1172, 1197, 1335, 1439
Alw21I GWGCWC 2 cut(s) 203, 363
Alw26I GTCTC 4 cut(s) 123, 772, 792, 1213
AlwI GGATC 5 cut(s) 833, 846, 1079, 1087, 1094
Aor13HI TCCGGA 1 cut(s) 888
AoxI GGCC 8 cut(s) 237, 277, 339, 540, 666, 692, 853, 1453
ApeKI GCWGC 3 cut(s) 457, 572, 971
ApoI RAATTY 2 cut(s) 619, 643
ArsI GACNNNNNNTTYG 2 cut(s) 505, 537
Asp700I GAANNNNTTC 1 cut(s) 994
AspA2I CCTAGG 1 cut(s) 274
AspLEI GCGC 2 cut(s) 173, 1380
AspS9I GGNCC 2 cut(s) 692, 1453
AsuHPI GGTGA 6 cut(s) 40, 90, 96, 102, 206, 530
AsuII TTCGAA 1 cut(s) 192
AvrII CCTAGG 1 cut(s) 274
BaeGI GKGCMC 1 cut(s) 392
BalI TGGCCA 2 cut(s) 668, 855
BamHI GGATCC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 1359
Bbv12I GWGCWC 2 cut(s) 203, 363
BbvI GCAGC 3 cut(s) 444, 559, 958
BccI CCATC 7 cut(s) 312, 532, 717, 768, 850, 1320, 1368
BceAI ACGGC 6 cut(s) 100, 142, 224, 312, 326, 527
BciT130I CCWGG 2 cut(s) 1069, 1413
BcoDI GTCTC 4 cut(s) 123, 772, 792, 1213
BfaI CTAG 3 cut(s) 275, 1107, 1173
BfoI RGCGCY 1 cut(s) 1381
BfrI CTTAAG 1 cut(s) 461
BfuAI ACCTGC 4 cut(s) 59, 176, 592, 938
BisI GCNGC 6 cut(s) 55, 349, 447, 458, 573, 972
BlnI CCTAGG 1 cut(s) 274
BlsI GCNGC 6 cut(s) 56, 350, 448, 459, 574, 973
Bme1390I CCNGG 2 cut(s) 1069, 1413
BmeRI GACNNNNNGTC 1 cut(s) 1360
BmgT120I GGNCC 2 cut(s) 692, 1453
BmiI GGNNCC 3 cut(s) 326, 840, 1454
BmrFI CCNGG 2 cut(s) 1069, 1413
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 6 cut(s) 929, 1111, 1126, 1133, 1218, 1434
BmuI ACTGGG 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 1359
Bpu14I TTCGAA 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 1235
BsaI GGTCTC 1 cut(s) 1213
BsaJI CCNNGG 7 cut(s) 274, 447, 663, 695, 1223, 1393, 1411
BsaWI WCCGGW 1 cut(s) 888
BsaXI ACNNNNNCTCC 6 cut(s) 70, 100, 909, 936, 939, 966
Bsc4I CCNNNNNNNGG 3 cut(s) 371, 888, 1112
Bse1I ACTGG 4 cut(s) 64, 217, 520, 846
Bse3DI GCAATG 2 cut(s) 1010, 1139
BseAI TCCGGA 1 cut(s) 888
BseBI CCWGG 2 cut(s) 1069, 1413
BseDI CCNNGG 7 cut(s) 274, 447, 663, 695, 1223, 1393, 1411
BseGI GGATG 8 cut(s) 323, 430, 842, 1126, 1216, 1233, 1331, 1379
BseLI CCNNNNNNNGG 3 cut(s) 371, 888, 1112
BseMI GCAATG 2 cut(s) 1010, 1139
BseMII CTCAG 1 cut(s) 1176
BseNI ACTGG 4 cut(s) 64, 217, 520, 846
BseRI GAGGAG 1 cut(s) 24
BseSI GKGCMC 1 cut(s) 392
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 3 cut(s) 444, 559, 958
BsgI GTGCAG 3 cut(s) 462, 554, 620
Bsh1236I CGCG 3 cut(s) 57, 348, 449
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 8 cut(s) 239, 279, 341, 542, 668, 694, 855, 1455
BshNI GGYRCC 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 2 cut(s) 203, 363
BsiSI CCGG 3 cut(s) 204, 236, 889
BslFI GGGAC 4 cut(s) 46, 256, 526, 893
BslI CCNNNNNNNGG 3 cut(s) 371, 888, 1112
BsmAI GTCTC 4 cut(s) 123, 772, 792, 1213
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 4 cut(s) 46, 256, 526, 893
BsnI GGCC 8 cut(s) 239, 279, 341, 542, 668, 694, 855, 1455
Bso31I GGTCTC 1 cut(s) 1213
Bsp119I TTCGAA 1 cut(s) 192
Bsp1286I GDGCHC 3 cut(s) 203, 363, 392
Bsp13I TCCGGA 1 cut(s) 888
Bsp143I GATC 4 cut(s) 838, 1071, 1079, 1099
Bsp19I CCATGG 2 cut(s) 1223, 1393
BspANI GGCC 8 cut(s) 239, 279, 341, 542, 668, 694, 855, 1455
BspCNI CTCAG 1 cut(s) 1177
BspEI TCCGGA 1 cut(s) 888
BspFNI CGCG 3 cut(s) 57, 348, 449
BspLI GGNNCC 3 cut(s) 326, 840, 1454
BspMI ACCTGC 4 cut(s) 59, 176, 592, 938
BspPI GGATC 5 cut(s) 833, 846, 1079, 1087, 1094
BspT104I TTCGAA 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 324
BspTI CTTAAG 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 1213
BsrDI GCAATG 2 cut(s) 1010, 1139
BsrI ACTGG 4 cut(s) 64, 217, 520, 846
BssECI CCNNGG 7 cut(s) 274, 447, 663, 695, 1223, 1393, 1411
BssMI GATC 4 cut(s) 838, 1071, 1079, 1099
BssT1I CCWWGG 5 cut(s) 274, 663, 695, 1223, 1393
Bst2UI CCWGG 2 cut(s) 1069, 1413
Bst4CI ACNGT 6 cut(s) 108, 198, 233, 302, 312, 557
Bst6I CTCTTC 2 cut(s) 570, 1211
BstAFI CTTAAG 1 cut(s) 461
BstAPI GCANNNNNTGC 1 cut(s) 926
BstBAI YACGTR 1 cut(s) 1235
BstBI TTCGAA 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 660, 692
BstDEI CTNAG 3 cut(s) 134, 482, 1185
BstDSI CCRYGG 3 cut(s) 447, 1223, 1393
BstF5I GGATG 8 cut(s) 323, 430, 842, 1126, 1216, 1233, 1331, 1379
BstFNI CGCG 3 cut(s) 57, 348, 449
BstH2I RGCGCY 1 cut(s) 1381
BstHHI GCGC 2 cut(s) 173, 1380
BstKTI GATC 4 cut(s) 841, 1074, 1082, 1102
BstMAI GTCTC 4 cut(s) 123, 772, 792, 1213
BstMBI GATC 4 cut(s) 838, 1071, 1079, 1099
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 5 cut(s) 338, 532, 892, 926, 1422
BstNI CCWGG 2 cut(s) 1069, 1413
BstNSI RCATGY 1 cut(s) 815
BstSCI CCNGG 2 cut(s) 1067, 1411
BstSLI GKGCMC 1 cut(s) 392
BstUI CGCG 3 cut(s) 57, 348, 449
BstV1I GCAGC 3 cut(s) 444, 559, 958
BstV2I GAAGAC 1 cut(s) 1359
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BstZI CGGCCG 1 cut(s) 237
BsuRI GGCC 8 cut(s) 239, 279, 341, 542, 668, 694, 855, 1455
BtgI CCRYGG 3 cut(s) 447, 1223, 1393
BtsCI GGATG 8 cut(s) 323, 430, 842, 1126, 1216, 1233, 1331, 1379
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 178
BveI ACCTGC 4 cut(s) 59, 176, 592, 938
Cac8I GCNNGC 2 cut(s) 660, 692
CfoI GCGC 2 cut(s) 173, 1380
Cfr13I GGNCC 2 cut(s) 692, 1453
Cfr42I CCGCGG 1 cut(s) 450
Csp6I GTAC 3 cut(s) 210, 558, 1088
CviAII CATG 4 cut(s) 812, 1075, 1224, 1394
CviQI GTAC 3 cut(s) 210, 558, 1088
DdeI CTNAG 3 cut(s) 134, 482, 1185
DpnI GATC 4 cut(s) 840, 1073, 1081, 1101
DpnII GATC 4 cut(s) 838, 1071, 1079, 1099
DraIII CACNNNGTG 1 cut(s) 724
DriI GACNNNNNGTC 1 cut(s) 1360
EaeI YGGCCR 5 cut(s) 237, 339, 540, 666, 853
EagI CGGCCG 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 570, 1211
Eam1105I GACNNNNNGTC 1 cut(s) 1360
EarI CTCTTC 2 cut(s) 570, 1211
EciI GGCGGA 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 237
Eco130I CCWWGG 5 cut(s) 274, 663, 695, 1223, 1393
Eco147I AGGCCT 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 1213
Eco52I CGGCCG 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 1260
EcoRI GAATTC 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 1067, 1411
EcoT14I CCWWGG 5 cut(s) 274, 663, 695, 1223, 1393
EcoT22I ATGCAT 2 cut(s) 1126, 1141
ErhI CCWWGG 5 cut(s) 274, 663, 695, 1223, 1393
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 4 cut(s) 815, 1078, 1227, 1397
FalI AAGNNNNNCTT 1 cut(s) 1487
FaqI GGGAC 4 cut(s) 46, 256, 526, 893
FatI CATG 4 cut(s) 811, 1074, 1223, 1393
FauI CCCGC 1 cut(s) 683
FauNDI CATATG 1 cut(s) 496
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 6 cut(s) 55, 349, 447, 458, 573, 972
FokI GGATG 8 cut(s) 330, 417, 829, 1133, 1223, 1240, 1338, 1386
Fsp4HI GCNGC 6 cut(s) 55, 349, 447, 458, 573, 972
FspBI CTAG 3 cut(s) 275, 1107, 1173
GlaI GCGC 2 cut(s) 172, 1379
GluI GCNGC 6 cut(s) 55, 349, 447, 458, 573, 972
HaeII RGCGCY 1 cut(s) 1381
HaeIII GGCC 8 cut(s) 239, 279, 341, 542, 668, 694, 855, 1455
HapII CCGG 3 cut(s) 204, 236, 889
HhaI GCGC 2 cut(s) 173, 1380
Hin1II CATG 4 cut(s) 815, 1078, 1227, 1397
Hin6I GCGC 2 cut(s) 171, 1378
HinP1I GCGC 2 cut(s) 171, 1378
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 2 cut(s) 1195, 1333
HinfI GANTC 2 cut(s) 715, 1247
HpaII CCGG 3 cut(s) 204, 236, 889
HphI GGTGA 6 cut(s) 40, 90, 96, 102, 206, 530
Hpy166II GTNNAC 2 cut(s) 247, 1465
Hpy188III TCNNGA 7 cut(s) 383, 413, 624, 889, 1118, 1447, 1485
Hpy8I GTNNAC 2 cut(s) 247, 1465
Hpy99I CGWCG 4 cut(s) 163, 245, 248, 251
HpyAV CCTTC 3 cut(s) 38, 99, 984
HpyCH4III ACNGT 6 cut(s) 108, 198, 233, 302, 312, 557
HpyCH4IV ACGT 1 cut(s) 1234
HpyF10VI GCNNNNNNNGC 5 cut(s) 338, 532, 892, 926, 1422
HpyF3I CTNAG 3 cut(s) 134, 482, 1185
HpySE526I ACGT 1 cut(s) 1234
Hsp92II CATG 4 cut(s) 815, 1078, 1227, 1397
HspAI GCGC 2 cut(s) 171, 1378
Kpn2I TCCGGA 1 cut(s) 888
KspI CCGCGG 1 cut(s) 450
Kzo9I GATC 4 cut(s) 838, 1071, 1079, 1099
LmnI GCTCC 6 cut(s) 206, 358, 808, 891, 917, 944
Lsp1109I GCAGC 3 cut(s) 444, 559, 958
LweI GCATC 6 cut(s) 929, 1111, 1126, 1133, 1218, 1434
MaeI CTAG 3 cut(s) 275, 1107, 1173
MaeII ACGT 1 cut(s) 1234
MaeIII GTNAC 4 cut(s) 96, 108, 544, 749
MalI GATC 4 cut(s) 840, 1073, 1081, 1101
MboI GATC 4 cut(s) 838, 1071, 1079, 1099
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 3 cut(s) 203, 363, 392
MlsI TGGCCA 2 cut(s) 668, 855
MluCI AATT 7 cut(s) 335, 619, 643, 670, 822, 1251, 1402
MluNI TGGCCA 2 cut(s) 668, 855
MmeI TCCRAC 1 cut(s) 796
Mox20I TGGCCA 2 cut(s) 668, 855
Mph1103I ATGCAT 2 cut(s) 1126, 1141
MroI TCCGGA 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 994
MscI TGGCCA 2 cut(s) 668, 855
MseI TTAA 3 cut(s) 462, 1199, 1401
Msp20I TGGCCA 2 cut(s) 668, 855
MspA1I CMGCKG 1 cut(s) 449
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 3 cut(s) 204, 236, 889
MspR9I CCNGG 2 cut(s) 1069, 1413
MvaI CCWGG 2 cut(s) 1069, 1413
MvnI CGCG 3 cut(s) 57, 348, 449
MwoI GCNNNNNNNGC 5 cut(s) 338, 532, 892, 926, 1422
NcoI CCATGG 2 cut(s) 1223, 1393
NdeI CATATG 1 cut(s) 496
NdeII GATC 4 cut(s) 838, 1071, 1079, 1099
NlaIII CATG 4 cut(s) 815, 1078, 1227, 1397
NlaIV GGNNCC 3 cut(s) 326, 840, 1454
NmuCI GTSAC 3 cut(s) 96, 108, 544
NsiI ATGCAT 2 cut(s) 1126, 1141
NspI RCATGY 1 cut(s) 815
NspV TTCGAA 1 cut(s) 192
PaqCI CACCTGC 2 cut(s) 59, 938
PceI AGGCCT 1 cut(s) 279
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 994
PfeI GAWTC 2 cut(s) 715, 1247
PfoI TCCNGGA 1 cut(s) 1067
PkrI GCNGC 6 cut(s) 56, 350, 448, 459, 574, 973
Ppu21I YACGTR 1 cut(s) 1235
Psp6I CCWGG 2 cut(s) 1067, 1411
PspGI CCWGG 2 cut(s) 1067, 1411
PspN4I GGNNCC 3 cut(s) 326, 840, 1454
PspPI GGNCC 2 cut(s) 692, 1453
PsuI RGATCY 1 cut(s) 838
RsaI GTAC 3 cut(s) 211, 559, 1089
RsaNI GTAC 3 cut(s) 210, 558, 1088
SacII CCGCGG 1 cut(s) 450
SalI GTCGAC 1 cut(s) 245
SaqAI TTAA 3 cut(s) 462, 1199, 1401
SatI GCNGC 6 cut(s) 55, 349, 447, 458, 573, 972
Sau3AI GATC 4 cut(s) 838, 1071, 1079, 1099
Sau96I GGNCC 2 cut(s) 692, 1453
ScrFI CCNGG 2 cut(s) 1069, 1413
SduI GDGCHC 3 cut(s) 203, 363, 392
SfaNI GCATC 6 cut(s) 929, 1111, 1126, 1133, 1218, 1434
Sfr303I CCGCGG 1 cut(s) 450
SfuI TTCGAA 1 cut(s) 192
SgrBI CCGCGG 1 cut(s) 450
SgrDI CGTCGACG 1 cut(s) 245
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 7 cut(s) 335, 619, 643, 670, 822, 1251, 1402
SseBI AGGCCT 1 cut(s) 279
SspMI CTAG 3 cut(s) 275, 1107, 1173
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 1067, 1411
StyI CCWWGG 5 cut(s) 274, 663, 695, 1223, 1393
TaaI ACNGT 6 cut(s) 108, 198, 233, 302, 312, 557
TaiI ACGT 1 cut(s) 1237
TaqI TCGA 5 cut(s) 192, 246, 297, 623, 1428
TasI AATT 7 cut(s) 335, 619, 643, 670, 822, 1251, 1402
TatI WGTACW 3 cut(s) 209, 557, 1087
TauI GCSGC 3 cut(s) 57, 351, 449
TfiI GAWTC 2 cut(s) 715, 1247
Tru1I TTAA 3 cut(s) 462, 1199, 1401
Tru9I TTAA 3 cut(s) 462, 1199, 1401
TscAI CASTG 1 cut(s) 185
TseFI GTSAC 3 cut(s) 96, 108, 544
TseI GCWGC 3 cut(s) 457, 572, 971
Tsp45I GTSAC 3 cut(s) 96, 108, 544
TspDTI ATGAA 3 cut(s) 516, 645, 822
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 1 cut(s) 185
Vha464I CTTAAG 1 cut(s) 461
XapI RAATTY 2 cut(s) 619, 643
XceI RCATGY 1 cut(s) 815
XmaJI CCTAGG 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 994
XspI CTAG 3 cut(s) 275, 1107, 1173
Zsp2I ATGCAT 2 cut(s) 1126, 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.