MD09G1012500.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013116
Physical Location & Seq
Reverse (-)
798005 .. 802040
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1012500.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGCCTCTCTGCTTCCTCTCCGTCCGTCCTCCTCCGCCTCCACTCATCACCTGCCACGTGTCCCCAACCGCAGTGCGTCCCCCGCCGTCTGCTTCAGCACCACCACAAGTACCAGCAGCACTACCTTCTCTCGCACTGAGCCCTAGCTCAACCCCTCCTCCGCCGCCGCTAACCTGCGCCCTCCACTGCCCGCACTTCGAATCGTGCTCCGGGTGTACGCACGAGTTCAATCTCCACCGTCCGGTCATCGTCGATGAGGCAACGGAGTTCTTCAAGACAGTTGGAGTCTCAGATTTCACTTTTGACAGTTGCAAATTGTGGGGATGGCGGTGTCGCGCAAAGTTAGCCGTTCGTGGCTCACCGGAAAACCCCCTGATTGGGCTTTATCAGGAGGGAACTCATAATGTAGTAGATATTCCTCAATGCAAAGCTCATCATCCAAGTATAAATGCTGCTGTGGAGCTGCTTAAACAAGGTATTACTGAACTACGCGTTGAGCCATATGATGAAGATGAGGGGACAGGTGATTTACGATATGTGCAGATGGCTGTGACAACACACAATACTTCCCTTCCCGCTTCCGAAAGATATAAGAATGGTAAAGTGCAGGTAGCTTTAGTATGGAATTCAAGAAACGAAAATTCGCCGAGTTCGGAGAAACTGCAAGCCTTGGCCAATTTTATGTGGAGAAATGGTGGGCCAAGGAACAAGGTCAATCTGATTCACTCTGTATGGGCTAACTTTCAGACATCATCTAACAATATAATTTTTGGAAATAGATGGAGACATCTTTTAGGGGAAAGAGACTTTTGGGAACATGTTGGAGGAATTGATATTTCCTTGGCTCCGTCCAGTTTTGGCCAAGCAAACACAAGGGCTTTTGATACCTTGCTTCGGAAGCTACAAAAATATGTCCCTCAGGGAGCATCTGTTGCTGATTTGTATGCAGGAGCTGGTGTAATTGGATTATCATTAGCTGTGACAAGAAAATGCAGGTCTGTTCGATGCATTGAGATTAATAAAGAATCAAAAGTATCCTTTGAGAAGACAGTTGACCGCCTGCCAAACTCGGTAGAGAGCAGCATCAGCTGGCATCTTGCAGACACTTCTAAAGAACCTCTTTCTTGGATCATGGGATCAGATGTACTTGTTGTCGATCCTCCTAGAAAGGGACTGGATGCATCTCTTGTTGATGCATTGCGGAGTATAGCTTCAGCAAAGCGTAAAGCTAAGTCGTCCTCGGAGAGTTCAAGCTCAAGGGGTAAGGATGAGAAGAGACCATGGATGCTGCGTGCAAAAGAAGCTTCAGTTCAGATCAGAAGCGAAATTACTTCAGAAGATAGTAAATCATGGCCTCAAACCCTTATTTATATAAGTTGTGGATGGGAAAGCTTCAAAGAGGATTGCAAGGATTTGTTGTCTAGCAAGGCATGGCACTTGGAAAAAGCCCATGGCTATAATTTTTTCCCTGGAACCCAGAGCATCGAGGTTTTAGCTGTGTTCAAGAGGGGCCAAGGAAGCCCAAAGAAAAAGAGAACAGGAATAAAGCAGAAGAAACGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

522

Amino Acids

57.64

Weight (kDa)

9.21

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 59
Acc36I ACCTGC 4 cut(s) 59, 182, 598, 984
AccII CGCG 2 cut(s) 336, 492
AclWI GGATC 3 cut(s) 1136, 1144, 1151
AcoI YGGCCR 2 cut(s) 672, 859
AcsI RAATTY 2 cut(s) 625, 640
AcuI CTGAAG 4 cut(s) 78, 1197, 1290, 1317
AcvI CACGTG 1 cut(s) 58
AfaI GTAC 3 cut(s) 111, 217, 1146
AfiI CCNNNNNNNGG 5 cut(s) 241, 377, 378, 894, 1169
AflIII ACRYGT 3 cut(s) 57, 490, 817
AgsI TTSAA 6 cut(s) 229, 274, 630, 1251, 1396, 1504
AjnI CCWGG 1 cut(s) 1468
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 4 cut(s) 292, 778, 798, 1270
AlwI GGATC 3 cut(s) 1136, 1144, 1151
AlwNI CAGNNNCTG 1 cut(s) 953
AoxI GGCC 5 cut(s) 672, 698, 859, 1352, 1510
ApeKI GCWGC 5 cut(s) 116, 452, 463, 1080, 1288
ApoI RAATTY 2 cut(s) 625, 640
AseI ATTAAT 1 cut(s) 1017
AspLEI GCGC 2 cut(s) 179, 338
AspS9I GGNCC 2 cut(s) 698, 1510
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 3 cut(s) 40, 351, 536
AsuII TTCGAA 1 cut(s) 198
AxyI CCTNAGG 1 cut(s) 918
BalI TGGCCA 2 cut(s) 674, 861
BanII GRGCYC 1 cut(s) 143
BarI GAAGNNNNNNTAC 2 cut(s) 1312, 1344
BauI CACGAG 1 cut(s) 221
BbrPI CACGTG 1 cut(s) 58
BbsI GAAGAC 1 cut(s) 1052
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 5 cut(s) 128, 439, 450, 1092, 1275
BccI CCATC 4 cut(s) 318, 538, 774, 1377
BceAI ACGGC 2 cut(s) 70, 332
BciT130I CCWGG 1 cut(s) 1470
BciVI GTATCC 1 cut(s) 1045
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 4 cut(s) 292, 778, 798, 1270
BfaI CTAG 3 cut(s) 144, 1164, 1422
BfuAI ACCTGC 4 cut(s) 59, 182, 598, 984
BfuI GTATCC 1 cut(s) 1045
BisI GCNGC 7 cut(s) 117, 164, 167, 453, 464, 1081, 1289
BlsI GCNGC 7 cut(s) 118, 165, 168, 454, 465, 1082, 1290
Bme1390I CCNGG 2 cut(s) 211, 1470
BmgT120I GGNCC 2 cut(s) 698, 1510
BmiI GGNNCC 3 cut(s) 846, 1474, 1511
BmrFI CCNGG 2 cut(s) 211, 1470
BmsI GCATC 9 cut(s) 935, 995, 1092, 1102, 1168, 1183, 1190, 1275, 1491
BpiI GAAGAC 1 cut(s) 1052
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 1240
BpuMI CCSGG 1 cut(s) 211
BsaAI YACGTR 1 cut(s) 58
BsaI GGTCTC 1 cut(s) 1270
BsaJI CCNNGG 8 cut(s) 669, 701, 840, 1239, 1280, 1450, 1468, 1513
BsaWI WCCGGW 2 cut(s) 241, 361
BsaXI ACNNNNNCTCC 8 cut(s) 142, 172, 679, 709, 915, 942, 945, 972
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 377, 378, 894, 1169
Bse1I ACTGG 2 cut(s) 852, 1179
Bse21I CCTNAGG 1 cut(s) 918
Bse3DI GCAATG 1 cut(s) 1196
BseBI CCWGG 1 cut(s) 1470
BseDI CCNNGG 8 cut(s) 669, 701, 840, 1239, 1280, 1450, 1468, 1513
BseGI GGATG 6 cut(s) 329, 436, 1183, 1273, 1290, 1388
BseLI CCNNNNNNNGG 5 cut(s) 241, 377, 378, 894, 1169
BseMI GCAATG 1 cut(s) 1196
BseMII CTCAG 3 cut(s) 128, 303, 932
BseNI ACTGG 2 cut(s) 852, 1179
BseRI GAGGAG 2 cut(s) 21, 147
BseXI GCAGC 5 cut(s) 128, 439, 450, 1092, 1275
BsgI GTGCAG 2 cut(s) 560, 626
Bsh1236I CGCG 2 cut(s) 336, 492
BshFI GGCC 5 cut(s) 674, 700, 861, 1354, 1512
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 210, 242, 362
BslFI GGGAC 5 cut(s) 46, 63, 532, 899, 1185
BslI CCNNNNNNNGG 5 cut(s) 241, 377, 378, 894, 1169
BsmAI GTCTC 4 cut(s) 292, 778, 798, 1270
BsmFI GGGAC 5 cut(s) 46, 63, 532, 899, 1185
BsnI GGCC 5 cut(s) 674, 700, 861, 1354, 1512
Bso31I GGTCTC 1 cut(s) 1270
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 2 cut(s) 143, 209
Bsp143I GATC 4 cut(s) 1128, 1136, 1156, 1314
Bsp19I CCATGG 2 cut(s) 1280, 1450
BspANI GGCC 5 cut(s) 674, 700, 861, 1354, 1512
BspCNI CTCAG 3 cut(s) 129, 302, 931
BspFNI CGCG 2 cut(s) 336, 492
BspLI GGNNCC 3 cut(s) 846, 1474, 1511
BspMI ACCTGC 4 cut(s) 59, 182, 598, 984
BspPI GGATC 3 cut(s) 1136, 1144, 1151
BspT104I TTCGAA 1 cut(s) 198
BspTNI GGTCTC 1 cut(s) 1270
BsrDI GCAATG 1 cut(s) 1196
BsrI ACTGG 2 cut(s) 852, 1179
BssECI CCNNGG 8 cut(s) 669, 701, 840, 1239, 1280, 1450, 1468, 1513
BssMI GATC 4 cut(s) 1128, 1136, 1156, 1314
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 6 cut(s) 669, 701, 840, 1280, 1450, 1513
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 1470
Bst4CI ACNGT 4 cut(s) 239, 280, 308, 1051
Bst6I CTCTTC 1 cut(s) 1268
BstAPI GCANNNNNTGC 1 cut(s) 932
BstBAI YACGTR 1 cut(s) 58
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 5 cut(s) 191, 666, 1061, 1091, 1293
BstDEI CTNAG 4 cut(s) 137, 289, 918, 1230
BstDSI CCRYGG 2 cut(s) 1280, 1450
BstF5I GGATG 6 cut(s) 329, 436, 1183, 1273, 1290, 1388
BstFNI CGCG 2 cut(s) 336, 492
BstHHI GCGC 2 cut(s) 179, 338
BstKTI GATC 4 cut(s) 1131, 1139, 1159, 1317
BstMAI GTCTC 4 cut(s) 292, 778, 798, 1270
BstMBI GATC 4 cut(s) 1128, 1136, 1156, 1314
BstMWI GCNNNNNNNGC 7 cut(s) 82, 344, 898, 932, 1086, 1301, 1518
BstNI CCWGG 1 cut(s) 1470
BstNSI RCATGY 1 cut(s) 821
BstSCI CCNGG 2 cut(s) 209, 1468
BstUI CGCG 2 cut(s) 336, 492
BstV1I GCAGC 5 cut(s) 128, 439, 450, 1092, 1275
BstV2I GAAGAC 1 cut(s) 1052
Bsu36I CCTNAGG 1 cut(s) 918
BsuI GTATCC 1 cut(s) 1045
BsuRI GGCC 5 cut(s) 674, 700, 861, 1354, 1512
BtgI CCRYGG 2 cut(s) 1280, 1450
BtsCI GGATG 6 cut(s) 329, 436, 1183, 1273, 1290, 1388
BtsI GCAGTG 2 cut(s) 78, 184
BtsIMutI CAGTG 3 cut(s) 78, 134, 184
BveI ACCTGC 4 cut(s) 59, 182, 598, 984
Cac8I GCNNGC 5 cut(s) 191, 666, 1061, 1091, 1293
CaiI CAGNNNCTG 1 cut(s) 953
CfoI GCGC 2 cut(s) 179, 338
Cfr13I GGNCC 2 cut(s) 698, 1510
CseI GACGC 1 cut(s) 65
Csp6I GTAC 3 cut(s) 110, 216, 1145
CviAII CATG 6 cut(s) 818, 1132, 1281, 1350, 1431, 1451
CviQI GTAC 3 cut(s) 110, 216, 1145
DdeI CTNAG 4 cut(s) 137, 289, 918, 1230
DpnI GATC 4 cut(s) 1130, 1138, 1158, 1316
DpnII GATC 4 cut(s) 1128, 1136, 1156, 1314
EaeI YGGCCR 2 cut(s) 672, 859
Eam1104I CTCTTC 1 cut(s) 1268
EarI CTCTTC 1 cut(s) 1268
EciI GGCGGA 2 cut(s) 24, 150
Eco130I CCWWGG 6 cut(s) 669, 701, 840, 1280, 1450, 1513
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 1270
Eco57I CTGAAG 4 cut(s) 78, 1197, 1290, 1317
Eco72I CACGTG 1 cut(s) 58
Eco81I CCTNAGG 1 cut(s) 918
EcoRI GAATTC 1 cut(s) 625
EcoRII CCWGG 1 cut(s) 1468
EcoT14I CCWWGG 6 cut(s) 669, 701, 840, 1280, 1450, 1513
EcoT22I ATGCAT 3 cut(s) 1010, 1183, 1198
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 6 cut(s) 669, 701, 840, 1280, 1450, 1513
FaeI CATG 6 cut(s) 821, 1135, 1284, 1353, 1434, 1454
FalI AAGNNNNNCTT 3 cut(s) 1104, 1136, 1544
FaqI GGGAC 5 cut(s) 46, 63, 532, 899, 1185
FatI CATG 6 cut(s) 817, 1131, 1280, 1349, 1430, 1450
FauI CCCGC 3 cut(s) 90, 198, 583
FauNDI CATATG 1 cut(s) 502
Fnu4HI GCNGC 7 cut(s) 117, 164, 167, 453, 464, 1081, 1289
FokI GGATG 6 cut(s) 336, 423, 1190, 1280, 1297, 1395
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 7 cut(s) 117, 164, 167, 453, 464, 1081, 1289
FspBI CTAG 3 cut(s) 144, 1164, 1422
GlaI GCGC 2 cut(s) 178, 337
GluI GCNGC 7 cut(s) 117, 164, 167, 453, 464, 1081, 1289
HaeIII GGCC 5 cut(s) 674, 700, 861, 1354, 1512
HapII CCGG 3 cut(s) 210, 242, 362
HgaI GACGC 1 cut(s) 65
HhaI GCGC 2 cut(s) 179, 338
Hin1II CATG 6 cut(s) 821, 1135, 1284, 1353, 1434, 1454
Hin6I GCGC 2 cut(s) 177, 336
HinP1I GCGC 2 cut(s) 177, 336
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 2 cut(s) 1302, 1390
HinfI GANTC 4 cut(s) 200, 285, 721, 1025
HpaII CCGG 3 cut(s) 210, 242, 362
HphI GGTGA 3 cut(s) 40, 351, 536
Hpy166II GTNNAC 2 cut(s) 216, 1054
Hpy188III TCNNGA 4 cut(s) 274, 389, 630, 1504
Hpy8I GTNNAC 2 cut(s) 216, 1054
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 2 cut(s) 135, 581
HpyCH4III ACNGT 4 cut(s) 239, 280, 308, 1051
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 7 cut(s) 82, 344, 898, 932, 1086, 1301, 1518
HpyF3I CTNAG 4 cut(s) 137, 289, 918, 1230
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 6 cut(s) 821, 1135, 1284, 1353, 1434, 1454
HspAI GCGC 2 cut(s) 177, 336
Kzo9I GATC 4 cut(s) 1128, 1136, 1156, 1314
LmnI GCTCC 5 cut(s) 212, 460, 850, 923, 950
Lsp1109I GCAGC 5 cut(s) 128, 439, 450, 1092, 1275
LweI GCATC 9 cut(s) 935, 995, 1092, 1102, 1168, 1183, 1190, 1275, 1491
MaeI CTAG 3 cut(s) 144, 1164, 1422
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 2 cut(s) 550, 979
MalI GATC 4 cut(s) 1130, 1138, 1158, 1316
MboI GATC 4 cut(s) 1128, 1136, 1156, 1314
MboII GAAGA 6 cut(s) 262, 521, 1057, 1285, 1349, 1564
MhlI GDGCHC 2 cut(s) 143, 209
MlsI TGGCCA 2 cut(s) 674, 861
MluCI AATT 9 cut(s) 314, 625, 640, 676, 765, 828, 960, 1326, 1459
MluI ACGCGT 1 cut(s) 490
MluNI TGGCCA 2 cut(s) 674, 861
MlyI GAGTC 1 cut(s) 294
MmeI TCCRAC 2 cut(s) 262, 802
Mox20I TGGCCA 2 cut(s) 674, 861
Mph1103I ATGCAT 3 cut(s) 1010, 1183, 1198
MscI TGGCCA 2 cut(s) 674, 861
MseI TTAA 2 cut(s) 468, 1017
Msp20I TGGCCA 2 cut(s) 674, 861
MspA1I CMGCKG 1 cut(s) 1089
MspI CCGG 3 cut(s) 210, 242, 362
MspR9I CCNGG 2 cut(s) 211, 1470
MvaI CCWGG 1 cut(s) 1470
MvnI CGCG 2 cut(s) 336, 492
MwoI GCNNNNNNNGC 7 cut(s) 82, 344, 898, 932, 1086, 1301, 1518
NciI CCSGG 1 cut(s) 211
NcoI CCATGG 2 cut(s) 1280, 1450
NdeI CATATG 1 cut(s) 502
NdeII GATC 4 cut(s) 1128, 1136, 1156, 1314
NlaIII CATG 6 cut(s) 821, 1135, 1284, 1353, 1434, 1454
NlaIV GGNNCC 3 cut(s) 846, 1474, 1511
NmeAIII GCCGAG 1 cut(s) 672
NmuCI GTSAC 2 cut(s) 550, 979
NsiI ATGCAT 3 cut(s) 1010, 1183, 1198
NspI RCATGY 1 cut(s) 821
NspV TTCGAA 1 cut(s) 198
PaqCI CACCTGC 1 cut(s) 59
PciI ACATGT 1 cut(s) 817
PfeI GAWTC 3 cut(s) 200, 721, 1025
PkrI GCNGC 7 cut(s) 118, 165, 168, 454, 465, 1082, 1290
PleI GAGTC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 58
PmlI CACGTG 1 cut(s) 58
PpsI GAGTC 1 cut(s) 293
Ppu21I YACGTR 1 cut(s) 58
PscI ACATGT 1 cut(s) 817
PshBI ATTAAT 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 1468
PspCI CACGTG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 1468
PspN4I GGNNCC 3 cut(s) 846, 1474, 1511
PspPI GGNCC 2 cut(s) 698, 1510
PstNI CAGNNNCTG 1 cut(s) 953
PvuII CAGCTG 1 cut(s) 1089
RsaI GTAC 3 cut(s) 111, 217, 1146
RsaNI GTAC 3 cut(s) 110, 216, 1145
SaqAI TTAA 2 cut(s) 468, 1017
SatI GCNGC 7 cut(s) 117, 164, 167, 453, 464, 1081, 1289
Sau3AI GATC 4 cut(s) 1128, 1136, 1156, 1314
Sau96I GGNCC 2 cut(s) 698, 1510
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 2 cut(s) 211, 1470
SduI GDGCHC 2 cut(s) 143, 209
SfaNI GCATC 9 cut(s) 935, 995, 1092, 1102, 1168, 1183, 1190, 1275, 1491
SfuI TTCGAA 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 1255
SmoI CTYRAG 1 cut(s) 1255
Sse9I AATT 9 cut(s) 314, 625, 640, 676, 765, 828, 960, 1326, 1459
SspMI CTAG 3 cut(s) 144, 1164, 1422
StyD4I CCNGG 2 cut(s) 209, 1468
StyI CCWWGG 6 cut(s) 669, 701, 840, 1280, 1450, 1513
TaaI ACNGT 4 cut(s) 239, 280, 308, 1051
TaiI ACGT 1 cut(s) 60
TaqI TCGA 5 cut(s) 198, 252, 1003, 1155, 1485
TasI AATT 9 cut(s) 314, 625, 640, 676, 765, 828, 960, 1326, 1459
TatI WGTACW 1 cut(s) 1144
TauI GCSGC 2 cut(s) 166, 169
TfiI GAWTC 3 cut(s) 200, 721, 1025
Tru1I TTAA 2 cut(s) 468, 1017
Tru9I TTAA 2 cut(s) 468, 1017
TscAI CASTG 3 cut(s) 78, 141, 191
TseFI GTSAC 2 cut(s) 550, 979
TseI GCWGC 5 cut(s) 116, 452, 463, 1080, 1288
Tsp45I GTSAC 2 cut(s) 550, 979
TspDTI ATGAA 1 cut(s) 522
TspGWI ACGGA 4 cut(s) 10, 14, 278, 837
TspRI CASTG 3 cut(s) 78, 141, 191
VspI ATTAAT 1 cut(s) 1017
XapI RAATTY 2 cut(s) 625, 640
XceI RCATGY 1 cut(s) 821
XspI CTAG 3 cut(s) 144, 1164, 1422
Zsp2I ATGCAT 3 cut(s) 1010, 1183, 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.