Rmu_sc0003512.1_g000009
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003512.1
OG0013116
Physical Location & Seq
Forward (+)
39142 .. 42650
3509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003512.1_g000009.1.cds

Sequence Viewer

Length: 1578 bp
atgcctctctgcttcctctccccccgccttcctcctccgccactcatcacctgccgcgtgtcccaatcctcactccgcaccctacttccttctccttctccttcaccgtcaccgccaccgccaccgtcaccgtcgtctctctcactctcactaagccctaactcaaccccaccgccgtcgctaacctgcgccctccactgcccacacttcgaactgtgctccggctgtactcacgagttcaatctccaccgtccggccatcgtcgacgaggctaccaagttcttcaagtccctaggcctctccgatttcacattcgacagttgcaaactgtggggatggcggtgccgtgcgaaattggccgttcgcggctcctcggagcaccctctgattgggctgtatcaggagggcactcataatatagtcgatattcctgactgcaaagctcatcatccgaatatcaatgccgcggtagagctgcttaagaaagggattactgcgctaaggattgagccatatgatgaagatgaggggactggtgatttgcgatatgtgcagatggccgtgacaacatacagtacatctcttcctgctgccgaaagatatagaaatggtaaagtgcaggtatctttagtatggaattcgagaaatgaaacctccccaaattcagagaaactgcaagccttggccaattttatatggaggaatggtgggccaaggaacaatgtccatctgattcactctgtgtgggctaactttcagacatcaagtaacaatataatctttgggaatagatggagacatcttttaggagaaagagacttttgggagcatgtcggaggaattgatatttccttggctccatccagttttggccaagcaaacacacgggcatttgatacccttctccggaagctacaaaggtatgtccctcttggagcatctgttgctgatttgtatgcaggagcaggtgttatcggattatcattggctgctacaagaaaatgcaggtctgtgagatgcattgagattaacaaagaatcaaagctatcctttgagaagacggttgaccgcctgccaaacatagtagatagcagcatcagctggcatcatgcagacacttctaaggaacctctttcctggatcatgggatcagatgtacttgttgttgatcctcctagaaagggtctggatgcatctcttgttgatgcattgcggagtatatcttctataaaggataaagctaggtcatcatctgagagttcaagctcaaaggagaaggatgagaagaggccatggatgctacgtgcaagagaagattcaattcagattggaagcaaaatgacttcagaggatagcaaaatgttgcctaaaaccctcatttatataagttgtggatgggaaagcttcaaagaggattgtgaagacttgttgtcaagtaagggatggcgcttggaaaaagcccttggctttaattttttccctggcacacagagcatcgaggttttagctgtgttcaagaggggccaatcagtaaacccaaagaaaaagaaatcagggaagaagaagaaacgcttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

525

Amino Acids

58.3

Weight (kDa)

9.25

Isoelectric Point (pI)

55.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 59, 956
Acc36I ACCTGC 5 cut(s) 59, 194, 610, 956, 996
AccB1I GGYRCC 1 cut(s) 342
AccI GTMKAC 1 cut(s) 264
AccII CGCG 3 cut(s) 57, 366, 467
AccIII TCCGGA 1 cut(s) 906
AclWI GGATC 3 cut(s) 1148, 1156, 1163
AcoI YGGCCR 5 cut(s) 255, 357, 558, 684, 871
AcsI RAATTY 2 cut(s) 637, 661
AcuI CTGAAG 1 cut(s) 1329
AdeI CACNNNGTG 1 cut(s) 742
AfaI GTAC 3 cut(s) 229, 577, 1158
AfiI CCNNNNNNNGG 4 cut(s) 253, 389, 906, 1181
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 6 cut(s) 241, 286, 1263, 1320, 1408, 1516
AhdI GACNNNNNGTC 1 cut(s) 1429
AjnI CCWGG 2 cut(s) 1136, 1480
AjuI GAANNNNNNNTTGG 2 cut(s) 1446, 1478
AluBI AGCT 9 cut(s) 443, 475, 913, 1045, 1101, 1241, 1266, 1404, 1508
AluI AGCT 9 cut(s) 443, 475, 913, 1045, 1101, 1241, 1266, 1404, 1508
Alw21I GWGCWC 2 cut(s) 221, 381
Alw26I GTCTC 3 cut(s) 141, 790, 810
AlwI GGATC 3 cut(s) 1148, 1156, 1163
Aor13HI TCCGGA 1 cut(s) 906
AoxI GGCC 9 cut(s) 255, 295, 357, 558, 684, 710, 871, 1289, 1522
ApeKI GCWGC 4 cut(s) 475, 590, 989, 1092
ApoI RAATTY 2 cut(s) 637, 661
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
AspA2I CCTAGG 1 cut(s) 292
AspLEI GCGC 3 cut(s) 191, 499, 1449
AspS9I GGNCC 2 cut(s) 710, 1522
AsuHPI GGTGA 5 cut(s) 40, 96, 102, 120, 548
AsuII TTCGAA 1 cut(s) 210
AvrII CCTAGG 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 410
BalI TGGCCA 2 cut(s) 686, 873
BanI GGYRCC 1 cut(s) 342
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 2 cut(s) 1064, 1428
Bbv12I GWGCWC 2 cut(s) 221, 381
BbvI GCAGC 4 cut(s) 462, 577, 976, 1104
BccI CCATC 8 cut(s) 266, 330, 550, 735, 786, 868, 1389, 1437
BceAI ACGGC 4 cut(s) 160, 330, 344, 545
BciT130I CCWGG 2 cut(s) 1138, 1482
BcoDI GTCTC 3 cut(s) 141, 790, 810
BfaI CTAG 3 cut(s) 293, 1176, 1242
BfoI RGCGCY 1 cut(s) 1450
BfrI CTTAAG 1 cut(s) 479
BfuAI ACCTGC 5 cut(s) 59, 194, 610, 956, 996
BisI GCNGC 7 cut(s) 55, 367, 465, 476, 591, 990, 1093
BlnI CCTAGG 1 cut(s) 292
BlsI GCNGC 7 cut(s) 56, 368, 466, 477, 592, 991, 1094
Bme1390I CCNGG 2 cut(s) 1138, 1482
BmeRI GACNNNNNGTC 1 cut(s) 1429
BmgT120I GGNCC 2 cut(s) 710, 1522
BmiI GGNNCC 5 cut(s) 344, 370, 858, 1128, 1523
BmrFI CCNGG 2 cut(s) 1138, 1482
BmsI GCATC 9 cut(s) 947, 1007, 1104, 1114, 1180, 1195, 1202, 1287, 1503
BpiI GAAGAC 2 cut(s) 1064, 1428
Bpu10I CCTNAGC 1 cut(s) 500
Bpu14I TTCGAA 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 1304
BsaJI CCNNGG 9 cut(s) 292, 372, 465, 681, 713, 852, 1292, 1462, 1480
BsaWI WCCGGW 1 cut(s) 906
BsaXI ACNNNNNCTCC 6 cut(s) 691, 721, 927, 954, 957, 984
Bsc4I CCNNNNNNNGG 4 cut(s) 253, 389, 906, 1181
Bse1I ACTGG 2 cut(s) 538, 864
Bse3DI GCAATG 1 cut(s) 1208
BseAI TCCGGA 1 cut(s) 906
BseBI CCWGG 2 cut(s) 1138, 1482
BseDI CCNNGG 9 cut(s) 292, 372, 465, 681, 713, 852, 1292, 1462, 1480
BseGI GGATG 8 cut(s) 341, 448, 860, 1195, 1285, 1302, 1400, 1448
BseLI CCNNNNNNNGG 4 cut(s) 253, 389, 906, 1181
BseMI GCAATG 1 cut(s) 1208
BseMII CTCAG 1 cut(s) 1245
BseNI ACTGG 2 cut(s) 538, 864
BseRI GAGGAG 2 cut(s) 24, 361
BseSI GKGCMC 1 cut(s) 410
BseXI GCAGC 4 cut(s) 462, 577, 976, 1104
BsgI GTGCAG 2 cut(s) 572, 638
Bsh1236I CGCG 3 cut(s) 57, 366, 467
BshFI GGCC 9 cut(s) 257, 297, 359, 560, 686, 712, 873, 1291, 1524
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 221, 381
BsiSI CCGG 3 cut(s) 222, 254, 907
BslFI GGGAC 4 cut(s) 46, 274, 544, 911
BslI CCNNNNNNNGG 4 cut(s) 253, 389, 906, 1181
BsmAI GTCTC 3 cut(s) 141, 790, 810
BsmBI CGTCTC 1 cut(s) 141
BsmFI GGGAC 4 cut(s) 46, 274, 544, 911
BsnI GGCC 9 cut(s) 257, 297, 359, 560, 686, 712, 873, 1291, 1524
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 3 cut(s) 221, 381, 410
Bsp13I TCCGGA 1 cut(s) 906
Bsp143I GATC 3 cut(s) 1140, 1148, 1168
Bsp19I CCATGG 1 cut(s) 1292
BspANI GGCC 9 cut(s) 257, 297, 359, 560, 686, 712, 873, 1291, 1524
BspCNI CTCAG 1 cut(s) 1246
BspEI TCCGGA 1 cut(s) 906
BspFNI CGCG 3 cut(s) 57, 366, 467
BspLI GGNNCC 5 cut(s) 344, 370, 858, 1128, 1523
BspMI ACCTGC 5 cut(s) 59, 194, 610, 956, 996
BspPI GGATC 3 cut(s) 1148, 1156, 1163
BspT104I TTCGAA 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 1208
BsrI ACTGG 2 cut(s) 538, 864
BssECI CCNNGG 9 cut(s) 292, 372, 465, 681, 713, 852, 1292, 1462, 1480
BssMI GATC 3 cut(s) 1140, 1148, 1168
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 6 cut(s) 292, 681, 713, 852, 1292, 1462
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 2 cut(s) 1138, 1482
Bst4CI ACNGT 9 cut(s) 108, 126, 132, 216, 251, 320, 330, 575, 1063
Bst6I CTCTTC 2 cut(s) 588, 1280
BstAFI CTTAAG 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 944
BstBAI YACGTR 1 cut(s) 1304
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 678, 1073, 1103
BstDEI CTNAG 4 cut(s) 152, 500, 1122, 1254
BstDSI CCRYGG 2 cut(s) 465, 1292
BstF5I GGATG 8 cut(s) 341, 448, 860, 1195, 1285, 1302, 1400, 1448
BstFNI CGCG 3 cut(s) 57, 366, 467
BstH2I RGCGCY 1 cut(s) 1450
BstHHI GCGC 3 cut(s) 191, 499, 1449
BstKTI GATC 3 cut(s) 1143, 1151, 1171
BstMAI GTCTC 3 cut(s) 141, 790, 810
BstMBI GATC 3 cut(s) 1140, 1148, 1168
BstMWI GCNNNNNNNGC 6 cut(s) 356, 550, 944, 1098, 1297, 1491
BstNI CCWGG 2 cut(s) 1138, 1482
BstNSI RCATGY 1 cut(s) 833
BstSCI CCNGG 2 cut(s) 1136, 1480
BstSLI GKGCMC 1 cut(s) 410
BstUI CGCG 3 cut(s) 57, 366, 467
BstV1I GCAGC 4 cut(s) 462, 577, 976, 1104
BstV2I GAAGAC 2 cut(s) 1064, 1428
BsuRI GGCC 9 cut(s) 257, 297, 359, 560, 686, 712, 873, 1291, 1524
BtgI CCRYGG 2 cut(s) 465, 1292
BtsCI GGATG 8 cut(s) 341, 448, 860, 1195, 1285, 1302, 1400, 1448
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 196
BveI ACCTGC 5 cut(s) 59, 194, 610, 956, 996
Cac8I GCNNGC 3 cut(s) 678, 1073, 1103
CfoI GCGC 3 cut(s) 191, 499, 1449
Cfr13I GGNCC 2 cut(s) 710, 1522
Cfr42I CCGCGG 1 cut(s) 468
Csp6I GTAC 3 cut(s) 228, 576, 1157
CviAII CATG 4 cut(s) 830, 1109, 1144, 1293
CviQI GTAC 3 cut(s) 228, 576, 1157
DdeI CTNAG 4 cut(s) 152, 500, 1122, 1254
DpnI GATC 3 cut(s) 1142, 1150, 1170
DpnII GATC 3 cut(s) 1140, 1148, 1168
DraIII CACNNNGTG 1 cut(s) 742
DriI GACNNNNNGTC 1 cut(s) 1429
EaeI YGGCCR 5 cut(s) 255, 357, 558, 684, 871
Eam1104I CTCTTC 2 cut(s) 588, 1280
Eam1105I GACNNNNNGTC 1 cut(s) 1429
EarI CTCTTC 2 cut(s) 588, 1280
EciI GGCGGA 1 cut(s) 27
Eco130I CCWWGG 6 cut(s) 292, 681, 713, 852, 1292, 1462
Eco147I AGGCCT 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 1329
EcoRI GAATTC 1 cut(s) 637
EcoRII CCWGG 2 cut(s) 1136, 1480
EcoT14I CCWWGG 6 cut(s) 292, 681, 713, 852, 1292, 1462
EcoT22I ATGCAT 3 cut(s) 1022, 1195, 1210
ErhI CCWWGG 6 cut(s) 292, 681, 713, 852, 1292, 1462
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 4 cut(s) 833, 1112, 1147, 1296
FalI AAGNNNNNCTT 3 cut(s) 1034, 1066, 1556
FaqI GGGAC 4 cut(s) 46, 274, 544, 911
FatI CATG 4 cut(s) 829, 1108, 1143, 1292
FauI CCCGC 1 cut(s) 32
FauNDI CATATG 1 cut(s) 514
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 7 cut(s) 55, 367, 465, 476, 591, 990, 1093
FokI GGATG 8 cut(s) 348, 435, 847, 1202, 1292, 1309, 1407, 1455
Fsp4HI GCNGC 7 cut(s) 55, 367, 465, 476, 591, 990, 1093
FspBI CTAG 3 cut(s) 293, 1176, 1242
GlaI GCGC 3 cut(s) 190, 498, 1448
GluI GCNGC 7 cut(s) 55, 367, 465, 476, 591, 990, 1093
HaeII RGCGCY 1 cut(s) 1450
HaeIII GGCC 9 cut(s) 257, 297, 359, 560, 686, 712, 873, 1291, 1524
HapII CCGG 3 cut(s) 222, 254, 907
HhaI GCGC 3 cut(s) 191, 499, 1449
Hin1II CATG 4 cut(s) 833, 1112, 1147, 1296
Hin6I GCGC 3 cut(s) 189, 497, 1447
HinP1I GCGC 3 cut(s) 189, 497, 1447
HincII GTYRAC 2 cut(s) 265, 1066
HindII GTYRAC 2 cut(s) 265, 1066
HindIII AAGCTT 1 cut(s) 1402
HinfI GANTC 3 cut(s) 733, 1037, 1316
HpaII CCGG 3 cut(s) 222, 254, 907
HphI GGTGA 5 cut(s) 40, 96, 102, 120, 548
Hpy166II GTNNAC 3 cut(s) 265, 1066, 1534
Hpy188III TCNNGA 7 cut(s) 233, 401, 431, 642, 907, 1187, 1516
Hpy8I GTNNAC 3 cut(s) 265, 1066, 1534
Hpy99I CGWCG 4 cut(s) 136, 181, 266, 269
HpyAV CCTTC 6 cut(s) 38, 99, 105, 111, 911, 1270
HpyCH4III ACNGT 9 cut(s) 108, 126, 132, 216, 251, 320, 330, 575, 1063
HpyCH4IV ACGT 1 cut(s) 1303
HpyF10VI GCNNNNNNNGC 6 cut(s) 356, 550, 944, 1098, 1297, 1491
HpyF3I CTNAG 4 cut(s) 152, 500, 1122, 1254
HpySE526I ACGT 1 cut(s) 1303
Hsp92II CATG 4 cut(s) 833, 1112, 1147, 1296
HspAI GCGC 3 cut(s) 189, 497, 1447
Kpn2I TCCGGA 1 cut(s) 906
KspI CCGCGG 1 cut(s) 468
Kzo9I GATC 3 cut(s) 1140, 1148, 1168
LmnI GCTCC 7 cut(s) 224, 374, 376, 826, 862, 935, 962
Lsp1109I GCAGC 4 cut(s) 462, 577, 976, 1104
LweI GCATC 9 cut(s) 947, 1007, 1104, 1114, 1180, 1195, 1202, 1287, 1503
MaeI CTAG 3 cut(s) 293, 1176, 1242
MaeII ACGT 1 cut(s) 1303
MaeIII GTNAC 4 cut(s) 108, 126, 562, 767
MalI GATC 3 cut(s) 1142, 1150, 1170
MboI GATC 3 cut(s) 1140, 1148, 1168
MhlI GDGCHC 3 cut(s) 221, 381, 410
MlsI TGGCCA 2 cut(s) 686, 873
MluCI AATT 7 cut(s) 353, 637, 661, 688, 840, 1320, 1471
MluNI TGGCCA 2 cut(s) 686, 873
MmeI TCCRAC 1 cut(s) 814
Mox20I TGGCCA 2 cut(s) 686, 873
Mph1103I ATGCAT 3 cut(s) 1022, 1195, 1210
MroI TCCGGA 1 cut(s) 906
MscI TGGCCA 2 cut(s) 686, 873
MseI TTAA 3 cut(s) 480, 1029, 1470
Msp20I TGGCCA 2 cut(s) 686, 873
MspA1I CMGCKG 2 cut(s) 467, 1101
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 3 cut(s) 222, 254, 907
MspR9I CCNGG 2 cut(s) 1138, 1482
MvaI CCWGG 2 cut(s) 1138, 1482
MvnI CGCG 3 cut(s) 57, 366, 467
MwoI GCNNNNNNNGC 6 cut(s) 356, 550, 944, 1098, 1297, 1491
NcoI CCATGG 1 cut(s) 1292
NdeI CATATG 1 cut(s) 514
NdeII GATC 3 cut(s) 1140, 1148, 1168
NlaIII CATG 4 cut(s) 833, 1112, 1147, 1296
NlaIV GGNNCC 5 cut(s) 344, 370, 858, 1128, 1523
NmuCI GTSAC 3 cut(s) 108, 126, 562
NsiI ATGCAT 3 cut(s) 1022, 1195, 1210
NspI RCATGY 1 cut(s) 833
NspV TTCGAA 1 cut(s) 210
PaqCI CACCTGC 2 cut(s) 59, 956
PceI AGGCCT 1 cut(s) 297
PfeI GAWTC 3 cut(s) 733, 1037, 1316
PfoI TCCNGGA 1 cut(s) 1136
PkrI GCNGC 7 cut(s) 56, 368, 466, 477, 592, 991, 1094
Ppu21I YACGTR 1 cut(s) 1304
Psp6I CCWGG 2 cut(s) 1136, 1480
PspGI CCWGG 2 cut(s) 1136, 1480
PspN4I GGNNCC 5 cut(s) 344, 370, 858, 1128, 1523
PspPI GGNCC 2 cut(s) 710, 1522
PvuII CAGCTG 1 cut(s) 1101
RsaI GTAC 3 cut(s) 229, 577, 1158
RsaNI GTAC 3 cut(s) 228, 576, 1157
SacII CCGCGG 1 cut(s) 468
SalI GTCGAC 1 cut(s) 263
SaqAI TTAA 3 cut(s) 480, 1029, 1470
SatI GCNGC 7 cut(s) 55, 367, 465, 476, 591, 990, 1093
Sau3AI GATC 3 cut(s) 1140, 1148, 1168
Sau96I GGNCC 2 cut(s) 710, 1522
ScrFI CCNGG 2 cut(s) 1138, 1482
SduI GDGCHC 3 cut(s) 221, 381, 410
SfaNI GCATC 9 cut(s) 947, 1007, 1104, 1114, 1180, 1195, 1202, 1287, 1503
Sfr303I CCGCGG 1 cut(s) 468
SfuI TTCGAA 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 468
SgrDI CGTCGACG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 7 cut(s) 353, 637, 661, 688, 840, 1320, 1471
SseBI AGGCCT 1 cut(s) 297
SspMI CTAG 3 cut(s) 293, 1176, 1242
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 1136, 1480
StyI CCWWGG 6 cut(s) 292, 681, 713, 852, 1292, 1462
TaaI ACNGT 9 cut(s) 108, 126, 132, 216, 251, 320, 330, 575, 1063
TaiI ACGT 1 cut(s) 1306
TaqI TCGA 6 cut(s) 210, 264, 315, 423, 641, 1497
TasI AATT 7 cut(s) 353, 637, 661, 688, 840, 1320, 1471
TatI WGTACW 3 cut(s) 227, 575, 1156
TauI GCSGC 3 cut(s) 57, 369, 467
TfiI GAWTC 3 cut(s) 733, 1037, 1316
Tru1I TTAA 3 cut(s) 480, 1029, 1470
Tru9I TTAA 3 cut(s) 480, 1029, 1470
TscAI CASTG 1 cut(s) 203
TseFI GTSAC 3 cut(s) 108, 126, 562
TseI GCWGC 4 cut(s) 475, 590, 989, 1092
Tsp45I GTSAC 3 cut(s) 108, 126, 562
TspDTI ATGAA 2 cut(s) 534, 663
TspRI CASTG 1 cut(s) 203
Vha464I CTTAAG 1 cut(s) 479
XapI RAATTY 2 cut(s) 637, 661
XceI RCATGY 1 cut(s) 833
XmaJI CCTAGG 1 cut(s) 292
XmiI GTMKAC 1 cut(s) 264
XspI CTAG 3 cut(s) 293, 1176, 1242
Zsp2I ATGCAT 3 cut(s) 1022, 1195, 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.