AT3G28460

Conserved hypothetical protein 95

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009381
Physical Location & Seq
Reverse (-)
10672041 .. 10674456
2416 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28460.2

Sequence Viewer

Length: 945 bp
ATGGCGGTTTTGTTGTCATCTTTCTCTCCACTAGGAATCAACATTGACATGAATAAGAATCTGGGTGTTTCGTATTCTTCTTTCCCTCAAATTCATCTCTTTAAGGGTTCTACTCCACTGCGTGTTCGAACATTCGTCGTCTCGAGTCGTAAAAATTCAGGAACTGGTTTGGCAAGTGAGGATAAGAAATTGTTGCTGGAACGATATGGTTATGATGCTAATGATGACTTTGGGTCTCAATCTAAGAAGGCGAGAAGGAAAGAAGAGAAAATGAGTGGAAGGAATAGTCAACAAGTAGAAGAAGTTGTTGTTGTACAGCCAAGAACAACTCATAGGTTACTGCAGGTTCTTGCAGGAACGGCGAAACGAAAGAAATTGCTTTCTCTTAAGGGAATGGATGTGAGACCTATGATGGAAGTTGTTAAAGGAGCAGCTTTTGGAATTCTCCAGGCTGCTGGTGGTTGTCCAACGTCTCTGCGTCCTGGTAGGTGGTTGGATTTGTATAGTGGTACAGGGTCTGTTGGGATTGAAGCAATCAGTCGAGGATGTTCTGAGGCACATTTTGTTGAGATGGATCCTTGGGTTGTTTCAAATGTTCTACAACCAAACTTGGAACACACTGGGTTTGTTGATGCTTCGGTTATACACACTGCTCGAGTTGAAAACTTCCTGGAACGTGCTGATAAATTAGTAGGTAAGGATGGAGTGTTTGATTATATCAGCGTTACGCCACCTTACATGGAGGTTGATTATGAGGTTCTGATGGATCAGATTGCTAAGTCACCAGCGATTGGGGAAAATACTTTTATTTTGGTCGAGTACCCATCACGAACAACAATGCTTGATTCATGTGGATGCCTTGAAAAGATGACTGATAGAAGGTTTGGCCGGACACATCTAGCTATATATGGACCAAAATGGGCTCAGAAGCCAAGAAAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95

Protein Analysis

314

Amino Acids

34.81

Weight (kDa)

9.24

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cons_hypoth95 PF03602 114 - 271 1.6e-39 Conserved hypothetical protein 95
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 334
AccB7I CCANNNNNTGG 1 cut(s) 791
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 569, 582, 774
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 3 cut(s) 90, 154, 441
AdeI CACNNNGTG 1 cut(s) 122
AfaI GTAC 3 cut(s) 315, 511, 821
AfiI CCNNNNNNNGG 1 cut(s) 791
AflII CTTAAG 1 cut(s) 386
AgsI TTSAA 4 cut(s) 530, 591, 662, 863
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 3 cut(s) 447, 481, 669
AluBI AGCT 2 cut(s) 434, 902
AluI AGCT 2 cut(s) 434, 902
Alw26I GTCTC 4 cut(s) 145, 240, 397, 477
AlwI GGATC 3 cut(s) 569, 582, 774
AlwNI CAGNNNCTG 2 cut(s) 164, 518
Ama87I CYCGRG 2 cut(s) 142, 654
AoxI GGCC 1 cut(s) 886
ApeKI GCWGC 2 cut(s) 431, 452
ApoI RAATTY 3 cut(s) 90, 154, 441
AspS9I GGNCC 1 cut(s) 911
AsuHPI GGTGA 1 cut(s) 774
AsuII TTCGAA 1 cut(s) 127
AvaI CYCGRG 2 cut(s) 142, 654
AvaII GGWCC 1 cut(s) 911
BamHI GGATCC 1 cut(s) 574
BanII GRGCYC 1 cut(s) 925
BbvI GCAGC 2 cut(s) 439, 443
BccI CCATC 5 cut(s) 406, 565, 695, 757, 832
BceAI ACGGC 1 cut(s) 375
BciT130I CCWGG 3 cut(s) 449, 483, 671
BcoDI GTCTC 4 cut(s) 145, 240, 397, 477
BfaI CTAG 2 cut(s) 32, 899
BfmI CTRYAG 1 cut(s) 341
BfrI CTTAAG 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 2 cut(s) 432, 453
BlsI GCNGC 2 cut(s) 433, 454
Bme1390I CCNGG 3 cut(s) 449, 483, 671
Bme18I GGWCC 1 cut(s) 911
BmeRI GACNNNNNGTC 1 cut(s) 232
BmeT110I CYCGRG 2 cut(s) 142, 654
BmgT120I GGNCC 1 cut(s) 911
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 3 cut(s) 449, 483, 671
BmrI ACTGGG 1 cut(s) 630
BmsI GCATC 3 cut(s) 205, 622, 845
BmuI ACTGGG 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 431
Bpu14I TTCGAA 1 cut(s) 127
BsaI GGTCTC 2 cut(s) 240, 397
BsaJI CCNNGG 1 cut(s) 578
Bsc4I CCNNNNNNNGG 1 cut(s) 791
Bse1I ACTGG 2 cut(s) 169, 625
BseBI CCWGG 3 cut(s) 449, 483, 671
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 4 cut(s) 403, 551, 706, 860
BseLI CCNNNNNNNGG 1 cut(s) 791
BseMII CTCAG 2 cut(s) 543, 938
BseNI ACTGG 2 cut(s) 169, 625
BseXI GCAGC 2 cut(s) 439, 443
BshFI GGCC 1 cut(s) 888
BsiHKCI CYCGRG 2 cut(s) 142, 654
BsiSI CCGG 1 cut(s) 889
BslI CCNNNNNNNGG 1 cut(s) 791
BsmAI GTCTC 4 cut(s) 145, 240, 397, 477
BsmBI CGTCTC 2 cut(s) 145, 477
BsnI GGCC 1 cut(s) 888
Bso31I GGTCTC 2 cut(s) 240, 397
BsoBI CYCGRG 2 cut(s) 142, 654
Bsp119I TTCGAA 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 925
Bsp1407I TGTACA 1 cut(s) 313
Bsp143I GATC 2 cut(s) 574, 766
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 888
BspCNI CTCAG 2 cut(s) 544, 937
BspLI GGNNCC 1 cut(s) 576
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 3 cut(s) 569, 582, 774
BspT104I TTCGAA 1 cut(s) 127
BspTI CTTAAG 1 cut(s) 386
BspTNI GGTCTC 2 cut(s) 240, 397
BsrGI TGTACA 1 cut(s) 313
BsrI ACTGG 2 cut(s) 169, 625
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 2 cut(s) 574, 766
BssT1I CCWWGG 1 cut(s) 578
Bst2UI CCWGG 3 cut(s) 449, 483, 671
Bst6I CTCTTC 1 cut(s) 258
BstAFI CTTAAG 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 127
BstDEI CTNAG 4 cut(s) 243, 552, 777, 924
BstF5I GGATG 4 cut(s) 403, 551, 706, 860
BstKTI GATC 2 cut(s) 577, 769
BstMAI GTCTC 4 cut(s) 145, 240, 397, 477
BstMBI GATC 2 cut(s) 574, 766
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 3 cut(s) 449, 483, 671
BstSCI CCNGG 3 cut(s) 447, 481, 669
BstSFI CTRYAG 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 439, 443
BstX2I RGATCY 1 cut(s) 574
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 1 cut(s) 574
BsuRI GGCC 1 cut(s) 888
BtsCI GGATG 4 cut(s) 403, 551, 706, 860
BtsI GCAGTG 2 cut(s) 116, 648
BtsIMutI CAGTG 3 cut(s) 116, 618, 648
BveI ACCTGC 1 cut(s) 334
CaiI CAGNNNCTG 2 cut(s) 164, 518
Cfr13I GGNCC 1 cut(s) 911
CseI GACGC 1 cut(s) 467
Csp6I GTAC 3 cut(s) 314, 510, 820
CviAII CATG 3 cut(s) 49, 739, 849
CviJI RGCY 7 cut(s) 319, 434, 452, 888, 902, 923, 931
CviKI_1 RGCY 7 cut(s) 319, 434, 452, 888, 902, 923, 931
CviQI GTAC 3 cut(s) 314, 510, 820
DdeI CTNAG 4 cut(s) 243, 552, 777, 924
DpnI GATC 2 cut(s) 576, 768
DpnII GATC 2 cut(s) 574, 766
DraIII CACNNNGTG 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 1 cut(s) 258
Eam1105I GACNNNNNGTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 925
Eco31I GGTCTC 2 cut(s) 240, 397
Eco47I GGWCC 1 cut(s) 911
Eco88I CYCGRG 2 cut(s) 142, 654
EcoRI GAATTC 1 cut(s) 441
EcoRII CCWGG 3 cut(s) 447, 481, 669
EcoT14I CCWWGG 1 cut(s) 578
EcoT38I GRGCYC 1 cut(s) 925
ErhI CCWWGG 1 cut(s) 578
Esp3I CGTCTC 2 cut(s) 145, 477
FaeI CATG 3 cut(s) 52, 742, 852
FalI AAGNNNNNCTT 1 cut(s) 925
FatI CATG 3 cut(s) 48, 738, 848
Fnu4HI GCNGC 2 cut(s) 432, 453
FokI GGATG 4 cut(s) 410, 558, 713, 867
FriOI GRGCYC 1 cut(s) 925
Fsp4HI GCNGC 2 cut(s) 432, 453
FspBI CTAG 2 cut(s) 32, 899
GluI GCNGC 2 cut(s) 432, 453
GsuI CTGGAG 1 cut(s) 431
HaeIII GGCC 1 cut(s) 888
HapII CCGG 1 cut(s) 889
HgaI GACGC 1 cut(s) 467
Hin1II CATG 3 cut(s) 52, 742, 852
HincII GTYRAC 1 cut(s) 290
HindII GTYRAC 1 cut(s) 290
HinfI GANTC 4 cut(s) 36, 58, 145, 845
HpaII CCGG 1 cut(s) 889
HphI GGTGA 1 cut(s) 774
Hpy166II GTNNAC 1 cut(s) 290
Hpy188I TCNGA 4 cut(s) 553, 762, 771, 927
Hpy188III TCNNGA 3 cut(s) 142, 159, 828
Hpy8I GTNNAC 1 cut(s) 290
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 4 cut(s) 241, 249, 273, 873
HpyCH4IV ACGT 2 cut(s) 470, 676
HpyCH4V TGCA 2 cut(s) 343, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 4 cut(s) 243, 552, 777, 924
HpySE526I ACGT 2 cut(s) 470, 676
Hsp92II CATG 3 cut(s) 52, 742, 852
Kzo9I GATC 2 cut(s) 574, 766
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 2 cut(s) 439, 443
LweI GCATC 3 cut(s) 205, 622, 845
MaeI CTAG 2 cut(s) 32, 899
MaeII ACGT 2 cut(s) 470, 676
MaeIII GTNAC 3 cut(s) 336, 724, 780
MalI GATC 2 cut(s) 576, 768
MboI GATC 2 cut(s) 574, 766
MboII GAAGA 3 cut(s) 69, 275, 311
MflI RGATCY 1 cut(s) 574
MhlI GDGCHC 1 cut(s) 925
MluCI AATT 6 cut(s) 90, 154, 188, 374, 441, 686
MlyI GAGTC 1 cut(s) 154
MmeI TCCRAC 2 cut(s) 474, 491
MnlI CCTC 6 cut(s) 96, 172, 536, 547, 736, 748
MseI TTAA 4 cut(s) 102, 387, 423, 943
MslI CAYNNNNRTG 2 cut(s) 47, 853
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 1 cut(s) 889
MspR9I CCNGG 3 cut(s) 449, 483, 671
MvaI CCWGG 3 cut(s) 449, 483, 671
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeII GATC 2 cut(s) 574, 766
NlaIII CATG 3 cut(s) 52, 742, 852
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 1 cut(s) 780
NspV TTCGAA 1 cut(s) 127
PaeR7I CTCGAG 2 cut(s) 142, 654
PfeI GAWTC 3 cut(s) 36, 58, 845
PflMI CCANNNNNTGG 1 cut(s) 791
PfoI TCCNGGA 1 cut(s) 669
PkrI GCNGC 2 cut(s) 433, 454
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
Psp6I CCWGG 3 cut(s) 447, 481, 669
PspGI CCWGG 3 cut(s) 447, 481, 669
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 1 cut(s) 911
PspXI VCTCGAGB 1 cut(s) 654
PstI CTGCAG 1 cut(s) 345
PstNI CAGNNNCTG 2 cut(s) 164, 518
PsuI RGATCY 1 cut(s) 574
RsaI GTAC 3 cut(s) 315, 511, 821
RsaNI GTAC 3 cut(s) 314, 510, 820
RseI CAYNNNNRTG 2 cut(s) 47, 853
SaqAI TTAA 4 cut(s) 102, 387, 423, 943
SatI GCNGC 2 cut(s) 432, 453
Sau3AI GATC 2 cut(s) 574, 766
Sau96I GGNCC 1 cut(s) 911
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 3 cut(s) 449, 483, 671
SduI GDGCHC 1 cut(s) 925
SfaNI GCATC 3 cut(s) 205, 622, 845
SfcI CTRYAG 1 cut(s) 341
Sfr274I CTCGAG 2 cut(s) 142, 654
SfuI TTCGAA 1 cut(s) 127
SinI GGWCC 1 cut(s) 911
SlaI CTCGAG 2 cut(s) 142, 654
SmiMI CAYNNNNRTG 2 cut(s) 47, 853
SmlI CTYRAG 3 cut(s) 142, 386, 654
SmoI CTYRAG 3 cut(s) 142, 386, 654
Sse9I AATT 6 cut(s) 90, 154, 188, 374, 441, 686
SsiI CCGC 1 cut(s) 5
SspMI CTAG 2 cut(s) 32, 899
StyD4I CCNGG 3 cut(s) 447, 481, 669
StyI CCWWGG 1 cut(s) 578
TaiI ACGT 2 cut(s) 473, 679
TaqI TCGA 5 cut(s) 127, 143, 541, 655, 816
TasI AATT 6 cut(s) 90, 154, 188, 374, 441, 686
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 3 cut(s) 36, 58, 845
Tru1I TTAA 4 cut(s) 102, 387, 423, 943
Tru9I TTAA 4 cut(s) 102, 387, 423, 943
TscAI CASTG 3 cut(s) 123, 625, 655
TseFI GTSAC 1 cut(s) 780
TseI GCWGC 2 cut(s) 431, 452
Tsp45I GTSAC 1 cut(s) 780
TspDTI ATGAA 3 cut(s) 65, 83, 837
TspRI CASTG 3 cut(s) 123, 625, 655
Van91I CCANNNNNTGG 1 cut(s) 791
Vha464I CTTAAG 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 3 cut(s) 90, 154, 441
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XhoI CTCGAG 2 cut(s) 142, 654
XspI CTAG 2 cut(s) 32, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.