MD09G1042700.v1.1

rRNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009381
Physical Location & Seq
Reverse (-)
2756745 .. 2760836
4092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1042700.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGCGGTTTCATCTTCTCCGATCCTCTCTCCATTCCGAATGAAGATAACAGCTACGGAATTTGTCTCGCCTTCTTCCACTCTTCCTCGCATCAATTTCTGCAACAATCGACGGCAATCACCTGCCGTCGTCCTCGCTTCCAAGTCGGAGAAGGAATCGTGGAGTGAGGCCAAGAGAGTGTTGATGGAGCAGTACGGCTTCAACCCCGATGAATCCTTATCTCCTGAGCCCTCTCCAAACGGAAAGAGGAAGAAAAAGCTGAAAGAGTCTGGACGGGGCGTTGAAATTACACCAAAGGAGCCGAAGGCCGCTCGAACAACCCATAAGTTGCTTACTGTGCTTGGAGGAAAGGCGCGGAGAATGAAGTTGCTAAGTCCAAAAGGTATGGATGTGCGCCCGATGATGGAGGTTGTGAAAGGTGCAGCCTTTGATATCCTGCAGGCTGCTGGTGGTTGTCCTGCATCTTTGAGGCCTGGCCGTTGGTTAGACTTGTACAGTGGTACTGGATCGGTTGGAATTGAAGCTATTAGCCGAGGATGCTCCGAGGTACATTTTGTTGAGATGGATCCATGGGTTGTTTCAGATGTTTTACGCCCAAATTTGGAATGGACTGGGTTTCTTGATATGTCAGTTATACATACTGTTCGTGTTGAAAAATTCATGGAACGTGCACAGAAACTTGCAGGTAAAGAACCATTTGATTACATTAGCGTTACCCCACCGTATATGGAAGTTGACTATGCAGTACTGATGGATCAAATATCAAGTTCAGATCTAGTTGGAGAAGATAGCTTTATAGTAGTTGAGTACCCACTAAGGACTGACATGCTGGATTCATGTGGATGCCTTGTGAAGATAACCGATCGACGGTTTGGCAGGACACACTTGGCTATTTATGGACCCAAGTGGGCCCAGAAGAAGAAGAAGAAGAAAGAAAAGTCACTTCGAGGAGCAGCAGTAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95

Protein Analysis

323

Amino Acids

35.86

Weight (kDa)

9.42

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cons_hypoth95 PF03602 112 - 299 4.1e-45 Conserved hypothetical protein 95
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 130
Acc36I ACCTGC 2 cut(s) 130, 674
AccBSI CCGCTC 1 cut(s) 311
AccII CGCG 1 cut(s) 355
AciI CCGC 3 cut(s) 5, 309, 355
AclWI GGATC 5 cut(s) 16, 514, 560, 573, 762
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 4 cut(s) 59, 598, 656, 963
AfaI GTAC 6 cut(s) 194, 494, 502, 549, 747, 809
AfiI CCNNNNNNNGG 3 cut(s) 403, 601, 867
AgsI TTSAA 4 cut(s) 202, 284, 521, 653
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 4 cut(s) 53, 259, 524, 792
AluI AGCT 4 cut(s) 53, 259, 524, 792
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 1 cut(s) 70
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 5 cut(s) 16, 514, 560, 573, 762
AoxI GGCC 5 cut(s) 168, 306, 470, 475, 909
ApaI GGGCCC 1 cut(s) 913
ApaLI GTGCAC 1 cut(s) 669
ApeKI GCWGC 3 cut(s) 422, 443, 953
ApoI RAATTY 4 cut(s) 59, 598, 656, 963
AspLEI GCGC 2 cut(s) 355, 396
AspS9I GGNCC 3 cut(s) 899, 909, 910
AsuHPI GGTGA 1 cut(s) 111
AvaII GGWCC 1 cut(s) 899
BaeGI GKGCMC 2 cut(s) 673, 913
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 2 cut(s) 231, 913
Bbv12I GWGCWC 1 cut(s) 673
BbvI GCAGC 3 cut(s) 430, 434, 965
BccI CCATC 4 cut(s) 178, 397, 556, 745
BceAI ACGGC 4 cut(s) 110, 128, 211, 462
BciT130I CCWGG 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 1 cut(s) 776
BfmI CTRYAG 1 cut(s) 437
BfuAI ACCTGC 2 cut(s) 130, 674
BglII AGATCT 1 cut(s) 772
BisI GCNGC 4 cut(s) 309, 423, 444, 954
BlsI GCNGC 4 cut(s) 310, 424, 445, 955
BmcAI AGTACT 1 cut(s) 747
Bme1390I CCNGG 1 cut(s) 474
Bme18I GGWCC 1 cut(s) 899
BmgT120I GGNCC 3 cut(s) 899, 909, 910
BmiI GGNNCC 4 cut(s) 300, 567, 901, 911
BmrFI CCNGG 1 cut(s) 474
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 4 cut(s) 99, 470, 527, 833
BmuI ACTGGG 1 cut(s) 621
Bpu10I CCTNAGC 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 532, 543, 569
BsaXI ACNNNNNCTCC 1 cut(s) 942
Bsc4I CCNNNNNNNGG 3 cut(s) 403, 601, 867
Bse1I ACTGG 2 cut(s) 508, 616
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 3 cut(s) 532, 543, 569
BseGI GGATG 3 cut(s) 394, 542, 848
BseLI CCNNNNNNNGG 3 cut(s) 403, 601, 867
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 2 cut(s) 508, 616
BseRI GAGGAG 1 cut(s) 963
BseSI GKGCMC 2 cut(s) 673, 913
BseXI GCAGC 3 cut(s) 430, 434, 965
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 355
Bsh1285I CGRYCG 1 cut(s) 865
BshFI GGCC 5 cut(s) 170, 308, 472, 477, 911
BsiEI CGRYCG 1 cut(s) 865
BsiHKAI GWGCWC 1 cut(s) 673
BslI CCNNNNNNNGG 3 cut(s) 403, 601, 867
BsmAI GTCTC 1 cut(s) 70
BsnI GGCC 5 cut(s) 170, 308, 472, 477, 911
Bsp120I GGGCCC 1 cut(s) 909
Bsp1286I GDGCHC 3 cut(s) 231, 673, 913
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 6 cut(s) 21, 506, 565, 754, 772, 862
Bsp19I CCATGG 1 cut(s) 569
BspACI CCGC 3 cut(s) 5, 309, 355
BspANI GGCC 5 cut(s) 170, 308, 472, 477, 911
BspCNI CTCAG 1 cut(s) 217
BspFNI CGCG 1 cut(s) 355
BspLI GGNNCC 4 cut(s) 300, 567, 901, 911
BspMAI CTGCAG 1 cut(s) 441
BspMI ACCTGC 2 cut(s) 130, 674
BspPI GGATC 5 cut(s) 16, 514, 560, 573, 762
BsrBI CCGCTC 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 508, 616
BssECI CCNNGG 3 cut(s) 532, 543, 569
BssMI GATC 6 cut(s) 21, 506, 565, 754, 772, 862
BssT1I CCWWGG 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 5 cut(s) 337, 497, 643, 723, 870
Bst6I CTCTTC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 3 cut(s) 225, 371, 815
BstDSI CCRYGG 1 cut(s) 569
BstF5I GGATG 3 cut(s) 394, 542, 848
BstFNI CGCG 1 cut(s) 355
BstHHI GCGC 2 cut(s) 355, 396
BstKTI GATC 6 cut(s) 24, 509, 568, 757, 775, 865
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 6 cut(s) 21, 506, 565, 754, 772, 862
BstMCI CGRYCG 1 cut(s) 865
BstMWI GCNNNNNNNGC 2 cut(s) 337, 537
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 1 cut(s) 472
BstSFI CTRYAG 1 cut(s) 437
BstSLI GKGCMC 2 cut(s) 673, 913
BstUI CGCG 1 cut(s) 355
BstV1I GCAGC 3 cut(s) 430, 434, 965
BstX2I RGATCY 2 cut(s) 565, 772
BstYI RGATCY 2 cut(s) 565, 772
BsuRI GGCC 5 cut(s) 170, 308, 472, 477, 911
BtgI CCRYGG 1 cut(s) 569
BtsCI GGATG 3 cut(s) 394, 542, 848
BtsIMutI CAGTG 1 cut(s) 502
BveI ACCTGC 2 cut(s) 130, 674
Cac8I GCNNGC 1 cut(s) 441
CfoI GCGC 2 cut(s) 355, 396
Cfr13I GGNCC 3 cut(s) 899, 909, 910
Csp6I GTAC 6 cut(s) 193, 493, 501, 548, 746, 808
CviAII CATG 4 cut(s) 570, 661, 826, 837
CviQI GTAC 6 cut(s) 193, 493, 501, 548, 746, 808
DdeI CTNAG 3 cut(s) 225, 371, 815
DpnI GATC 6 cut(s) 23, 508, 567, 756, 774, 864
DpnII GATC 6 cut(s) 21, 506, 565, 754, 772, 862
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 569
Eco147I AGGCCT 1 cut(s) 472
Eco24I GRGCYC 2 cut(s) 231, 913
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 472
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 569
EcoT38I GRGCYC 2 cut(s) 231, 913
ErhI CCWWGG 1 cut(s) 569
FaeI CATG 4 cut(s) 573, 664, 829, 840
FatI CATG 4 cut(s) 569, 660, 825, 836
Fnu4HI GCNGC 4 cut(s) 309, 423, 444, 954
FokI GGATG 3 cut(s) 401, 549, 855
FriOI GRGCYC 2 cut(s) 231, 913
Fsp4HI GCNGC 4 cut(s) 309, 423, 444, 954
FspBI CTAG 1 cut(s) 776
GlaI GCGC 2 cut(s) 354, 395
GluI GCNGC 4 cut(s) 309, 423, 444, 954
HaeIII GGCC 5 cut(s) 170, 308, 472, 477, 911
HhaI GCGC 2 cut(s) 355, 396
Hin1II CATG 4 cut(s) 573, 664, 829, 840
Hin6I GCGC 2 cut(s) 353, 394
HinP1I GCGC 2 cut(s) 353, 394
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 4 cut(s) 155, 212, 266, 833
HphI GGTGA 1 cut(s) 111
Hpy166II GTNNAC 2 cut(s) 671, 736
Hpy188I TCNGA 6 cut(s) 21, 38, 148, 544, 583, 772
Hpy188III TCNNGA 3 cut(s) 224, 270, 620
Hpy8I GTNNAC 2 cut(s) 671, 736
Hpy99I CGWCG 3 cut(s) 114, 131, 870
HpyAV CCTTC 3 cut(s) 81, 145, 298
HpyCH4III ACNGT 5 cut(s) 337, 497, 643, 723, 870
HpyCH4IV ACGT 1 cut(s) 667
HpyCH4V TGCA 7 cut(s) 102, 422, 439, 461, 671, 683, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 337, 537
HpyF3I CTNAG 3 cut(s) 225, 371, 815
HpySE526I ACGT 1 cut(s) 667
Hsp92II CATG 4 cut(s) 573, 664, 829, 840
HspAI GCGC 2 cut(s) 353, 394
Kzo9I GATC 6 cut(s) 21, 506, 565, 754, 772, 862
LmnI GCTCC 4 cut(s) 187, 298, 545, 950
Lsp1109I GCAGC 3 cut(s) 430, 434, 965
LweI GCATC 4 cut(s) 99, 470, 527, 833
MaeI CTAG 1 cut(s) 776
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 2 cut(s) 712, 939
MalI GATC 6 cut(s) 23, 508, 567, 756, 774, 864
MbiI CCGCTC 1 cut(s) 311
MboI GATC 6 cut(s) 21, 506, 565, 754, 772, 862
MflI RGATCY 2 cut(s) 565, 772
MhlI GDGCHC 3 cut(s) 231, 673, 913
MluCI AATT 7 cut(s) 59, 94, 285, 516, 598, 656, 963
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 3 cut(s) 126, 493, 760
MslI CAYNNNNRTG 1 cut(s) 841
MspR9I CCNGG 1 cut(s) 474
MvaI CCWGG 1 cut(s) 474
MvnI CGCG 1 cut(s) 355
MwoI GCNNNNNNNGC 2 cut(s) 337, 537
NcoI CCATGG 1 cut(s) 569
NdeII GATC 6 cut(s) 21, 506, 565, 754, 772, 862
NlaIII CATG 4 cut(s) 573, 664, 829, 840
NlaIV GGNNCC 4 cut(s) 300, 567, 901, 911
NmeAIII GCCGAG 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 939
NspI RCATGY 1 cut(s) 829
PaqCI CACCTGC 1 cut(s) 130
PceI AGGCCT 1 cut(s) 472
PfeI GAWTC 3 cut(s) 155, 212, 833
PkrI GCNGC 4 cut(s) 310, 424, 445, 955
Ple19I CGATCG 1 cut(s) 865
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 4 cut(s) 300, 567, 901, 911
PspOMI GGGCCC 1 cut(s) 909
PspPI GGNCC 3 cut(s) 899, 909, 910
PstI CTGCAG 1 cut(s) 441
PsuI RGATCY 2 cut(s) 565, 772
PvuI CGATCG 1 cut(s) 865
RsaI GTAC 6 cut(s) 194, 494, 502, 549, 747, 809
RsaNI GTAC 6 cut(s) 193, 493, 501, 548, 746, 808
RseI CAYNNNNRTG 1 cut(s) 841
SatI GCNGC 4 cut(s) 309, 423, 444, 954
Sau3AI GATC 6 cut(s) 21, 506, 565, 754, 772, 862
Sau96I GGNCC 3 cut(s) 899, 909, 910
SbfI CCTGCAGG 1 cut(s) 441
ScaI AGTACT 1 cut(s) 747
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 474
SdaI CCTGCAGG 1 cut(s) 441
SduI GDGCHC 3 cut(s) 231, 673, 913
SfaNI GCATC 4 cut(s) 99, 470, 527, 833
SfcI CTRYAG 1 cut(s) 437
SinI GGWCC 1 cut(s) 899
SmiMI CAYNNNNRTG 1 cut(s) 841
Sse8387I CCTGCAGG 1 cut(s) 441
Sse9I AATT 7 cut(s) 59, 94, 285, 516, 598, 656, 963
SseBI AGGCCT 1 cut(s) 472
SsiI CCGC 3 cut(s) 5, 309, 355
SspMI CTAG 1 cut(s) 776
StuI AGGCCT 1 cut(s) 472
StyD4I CCNGG 1 cut(s) 472
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 5 cut(s) 337, 497, 643, 723, 870
TaiI ACGT 1 cut(s) 670
TaqI TCGA 4 cut(s) 109, 313, 865, 946
TasI AATT 7 cut(s) 59, 94, 285, 516, 598, 656, 963
TatI WGTACW 2 cut(s) 492, 745
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 3 cut(s) 155, 212, 833
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 1 cut(s) 939
TseI GCWGC 3 cut(s) 422, 443, 953
Tsp45I GTSAC 1 cut(s) 939
TspDTI ATGAA 5 cut(s) 56, 225, 377, 649, 825
TspGWI ACGGA 2 cut(s) 71, 255
TspRI CASTG 1 cut(s) 502
VneI GTGCAC 1 cut(s) 669
VpaK11BI GGWCC 1 cut(s) 899
XapI RAATTY 4 cut(s) 59, 598, 656, 963
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 603
XspI CTAG 1 cut(s) 776
ZrmI AGTACT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.