FvH4_6g49670

rRNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009381
Physical Location & Seq
Forward (+)
37162454 .. 37165149
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49670.t1

Sequence Viewer

Length: 972 bp
ATGGCGCTTTCATCGTCAACAATTCTATCTCCGTTCAGACTGAGCGTAACTCCAGACAACTCCGCCTCATCTTCAACCTCTCTCCCTCGCTTCAATTTCTCCTCCACCGCACCATGCACCAGTCGCCGACCTTCTTCACCTTTCGTCGTCCTCTCCTCAAAGTCCGGCGATCGAGCTTGGAACGATGCCAAGCGGCAGTTGATGGAGCAGTACGGCTTCGACCCCGACGTCGCCTTGACGGAGCCGACTCGCAAGGGAAAGAAGAAGAAGAGCAGTACTGAGCTACAGGAGGCCTCAGAGGCTGAGCCTAAGGCCAAGAGAACCACGCATAAGTTGCTTCAGGTGCTTGGAGGGAAGGCTCGGAGGATGAAGTTGCTTTCTCCAAAGGGAATGGATGTACGGCCGATGATGGAGGTTGTGAAAGGTGCAGCCTTTGACATCCTGCAGGCTGCAGGTGGCTGTCCTGCATCGTTAAGGCCTGGCCGTTGGTTAGACCTGTACAGTGGTACTGGATCTGTTGGAATAGAAGCTATTAGTCGAGGATGTTCTGAGGTACATTTTGTTGAGATGGATCCATGGGTCGTCTCAGAGGTTCTACGTCCAAACTTGGAATGGACAGGGTTCCTCGATGTTTCAGTTATACATGCTGTGCGTGTTGAAAAATTCATAGAACGTGCTGAGAAACTTGCAGGTAAAGAACCATTCGATTACATTAGTGTAACCCCTCCGTATACACAAGTTGACTATGCAGTACTGATGGATCAAATATCAAACTCGGCGTTAGTTGGGGAAAATACCTTTATAGTGGTTGAGTATGCAACGAGGACCGACATGCTTGATTCATGTGGATGTCTTGTGAAGATAACCGATCGGCGGTTTGGCAGGACACACTTGGTTATTTATGGACCTAAGTGGGCCCAGAAGAAGAAGAAATTAGAAAAGTCACCACGGGGAGTAGCAGTAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95

Protein Analysis

324

Amino Acids

35.66

Weight (kDa)

9.34

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cons_hypoth95 PF03602 113 - 301 5.4e-46 Conserved hypothetical protein 95
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 443
AasI GACNNNNNNGTC 1 cut(s) 227
AatII GACGTC 1 cut(s) 231
Acc36I ACCTGC 2 cut(s) 443, 680
AccI GTMKAC 1 cut(s) 731
AciI CCGC 4 cut(s) 63, 108, 193, 874
AclWI GGATC 4 cut(s) 520, 566, 579, 768
AcoI YGGCCR 2 cut(s) 401, 481
AcsI RAATTY 1 cut(s) 662
AcuI CTGAAG 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 7 cut(s) 212, 277, 399, 500, 508, 555, 753
AfiI CCNNNNNNNGG 1 cut(s) 873
AgsI TTSAA 3 cut(s) 75, 94, 659
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 3 cut(s) 176, 283, 530
AluI AGCT 3 cut(s) 176, 283, 530
Alw26I GTCTC 1 cut(s) 589
AlwI GGATC 4 cut(s) 520, 566, 579, 768
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 6 cut(s) 291, 312, 401, 476, 481, 915
ApaI GGGCCC 1 cut(s) 919
ApeKI GCWGC 2 cut(s) 428, 449
ApoI RAATTY 1 cut(s) 662
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 4 cut(s) 825, 905, 915, 916
AsuHPI GGTGA 2 cut(s) 129, 936
AvaII GGWCC 2 cut(s) 825, 905
AxyI CCTNAGG 1 cut(s) 309
BaeGI GKGCMC 1 cut(s) 919
BamHI GGATCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 919
BbvI GCAGC 2 cut(s) 436, 440
BccI CCATC 4 cut(s) 196, 403, 562, 751
BceAI ACGGC 3 cut(s) 229, 416, 468
BciT130I CCWGG 1 cut(s) 480
BcoDI GTCTC 1 cut(s) 589
BfmI CTRYAG 3 cut(s) 284, 443, 450
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 2 cut(s) 443, 680
BglI GCCNNNNNGGC 1 cut(s) 299
BisI GCNGC 3 cut(s) 194, 429, 450
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 3 cut(s) 195, 430, 451
BmcAI AGTACT 2 cut(s) 277, 753
Bme1390I CCNGG 1 cut(s) 480
Bme18I GGWCC 2 cut(s) 825, 905
BmgT120I GGNCC 4 cut(s) 825, 905, 915, 916
BmiI GGNNCC 4 cut(s) 243, 573, 623, 917
BmrFI CCNGG 1 cut(s) 480
BmsI GCATC 2 cut(s) 175, 476
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpmI CTGGAG 1 cut(s) 36
Bpu1102I GCTNAGC 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 575, 947
BsaXI ACNNNNNCTCC 1 cut(s) 945
Bsc4I CCNNNNNNNGG 1 cut(s) 873
Bse1I ACTGG 2 cut(s) 120, 514
Bse21I CCTNAGG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 2 cut(s) 575, 947
BseGI GGATG 5 cut(s) 372, 400, 438, 548, 854
BseLI CCNNNNNNNGG 1 cut(s) 873
BseMII CTCAG 7 cut(s) 32, 270, 294, 309, 540, 600, 669
BseNI ACTGG 2 cut(s) 120, 514
BseRI GAGGAG 2 cut(s) 91, 145
BseSI GKGCMC 1 cut(s) 919
BseX3I CGGCCG 1 cut(s) 401
BseXI GCAGC 2 cut(s) 436, 440
BsgI GTGCAG 1 cut(s) 447
Bsh1285I CGRYCG 3 cut(s) 172, 404, 871
BshFI GGCC 6 cut(s) 293, 314, 403, 478, 483, 917
BsiEI CGRYCG 3 cut(s) 172, 404, 871
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 873
BsmAI GTCTC 1 cut(s) 589
BsmBI CGTCTC 1 cut(s) 589
BsnI GGCC 6 cut(s) 293, 314, 403, 478, 483, 917
Bsp120I GGGCCC 1 cut(s) 915
Bsp1286I GDGCHC 1 cut(s) 919
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 5 cut(s) 169, 512, 571, 760, 868
Bsp1720I GCTNAGC 1 cut(s) 303
Bsp19I CCATGG 1 cut(s) 575
BspACI CCGC 4 cut(s) 63, 108, 193, 874
BspANI GGCC 6 cut(s) 293, 314, 403, 478, 483, 917
BspCNI CTCAG 7 cut(s) 33, 271, 295, 308, 541, 599, 670
BspLI GGNNCC 4 cut(s) 243, 573, 623, 917
BspMAI CTGCAG 2 cut(s) 447, 454
BspMI ACCTGC 2 cut(s) 443, 680
BspPI GGATC 4 cut(s) 520, 566, 579, 768
BspQI GCTCTTC 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 120, 514
BssECI CCNNGG 2 cut(s) 575, 947
BssMI GATC 5 cut(s) 169, 512, 571, 760, 868
BssNAI GTATAC 1 cut(s) 732
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 575
Bst1107I GTATAC 1 cut(s) 732
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 1 cut(s) 503
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 334
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 9 cut(s) 41, 279, 295, 303, 309, 549, 586, 678, 909
BstDSI CCRYGG 2 cut(s) 575, 947
BstF5I GGATG 5 cut(s) 372, 400, 438, 548, 854
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 172, 515, 574, 763, 871
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 5 cut(s) 169, 512, 571, 760, 868
BstMCI CGRYCG 3 cut(s) 172, 404, 871
BstMWI GCNNNNNNNGC 4 cut(s) 123, 299, 334, 343
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 2 cut(s) 647, 835
BstSCI CCNGG 1 cut(s) 478
BstSFI CTRYAG 3 cut(s) 284, 443, 450
BstSLI GKGCMC 1 cut(s) 919
BstV1I GCAGC 2 cut(s) 436, 440
BstX2I RGATCY 2 cut(s) 512, 571
BstYI RGATCY 2 cut(s) 512, 571
BstZ17I GTATAC 1 cut(s) 732
BstZI CGGCCG 1 cut(s) 401
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 6 cut(s) 293, 314, 403, 478, 483, 917
BtgI CCRYGG 2 cut(s) 575, 947
BtsCI GGATG 5 cut(s) 372, 400, 438, 548, 854
BtsIMutI CAGTG 1 cut(s) 508
BveI ACCTGC 2 cut(s) 443, 680
Cac8I GCNNGC 1 cut(s) 447
CaiI CAGNNNCTG 1 cut(s) 302
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 825, 905, 915, 916
Csp6I GTAC 7 cut(s) 211, 276, 398, 499, 507, 554, 752
CviAII CATG 5 cut(s) 114, 576, 644, 832, 843
CviQI GTAC 7 cut(s) 211, 276, 398, 499, 507, 554, 752
DdeI CTNAG 9 cut(s) 41, 279, 295, 303, 309, 549, 586, 678, 909
DpnI GATC 5 cut(s) 171, 514, 573, 762, 870
DpnII GATC 5 cut(s) 169, 512, 571, 760, 868
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 2 cut(s) 401, 481
EagI CGGCCG 1 cut(s) 401
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 1 cut(s) 52
EclXI CGGCCG 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 575
Eco147I AGGCCT 2 cut(s) 293, 478
Eco24I GRGCYC 1 cut(s) 919
Eco47I GGWCC 2 cut(s) 825, 905
Eco52I CGGCCG 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 323
Eco81I CCTNAGG 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 919
ErhI CCWWGG 1 cut(s) 575
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 5 cut(s) 117, 579, 647, 835, 846
FatI CATG 5 cut(s) 113, 575, 643, 831, 842
FblI GTMKAC 1 cut(s) 731
Fnu4HI GCNGC 3 cut(s) 194, 429, 450
FokI GGATG 5 cut(s) 379, 407, 425, 555, 861
FriOI GRGCYC 1 cut(s) 919
Fsp4HI GCNGC 3 cut(s) 194, 429, 450
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 194, 429, 450
GsuI CTGGAG 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 293, 314, 403, 478, 483, 917
HapII CCGG 1 cut(s) 165
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 5 cut(s) 117, 579, 647, 835, 846
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 18, 742
HindII GTYRAC 2 cut(s) 18, 742
HinfI GANTC 2 cut(s) 247, 839
HpaII CCGG 1 cut(s) 165
HphI GGTGA 2 cut(s) 129, 936
Hpy166II GTNNAC 3 cut(s) 18, 732, 742
Hpy188I TCNGA 5 cut(s) 38, 298, 363, 550, 589
Hpy188III TCNNGA 1 cut(s) 53
Hpy8I GTNNAC 3 cut(s) 18, 732, 742
Hpy99I CGWCG 3 cut(s) 149, 230, 233
HpyAV CCTTC 2 cut(s) 141, 349
HpyCH4III ACNGT 1 cut(s) 503
HpyCH4IV ACGT 3 cut(s) 228, 598, 673
HpyCH4V TGCA 8 cut(s) 117, 428, 445, 452, 467, 689, 749, 818
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 299, 334, 343
HpyF3I CTNAG 9 cut(s) 41, 279, 295, 303, 309, 549, 586, 678, 909
HpySE526I ACGT 3 cut(s) 228, 598, 673
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 5 cut(s) 117, 579, 647, 835, 846
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 5 cut(s) 169, 512, 571, 760, 868
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 2 cut(s) 205, 241
Lsp1109I GCAGC 2 cut(s) 436, 440
LweI GCATC 2 cut(s) 175, 476
MaeII ACGT 3 cut(s) 228, 598, 673
MaeIII GTNAC 3 cut(s) 46, 718, 942
MalI GATC 5 cut(s) 171, 514, 573, 762, 870
MboI GATC 5 cut(s) 169, 512, 571, 760, 868
MboII GAAGA 9 cut(s) 63, 126, 274, 277, 280, 871, 934, 937, 940
MflI RGATCY 2 cut(s) 512, 571
MhlI GDGCHC 1 cut(s) 919
MluCI AATT 5 cut(s) 21, 94, 662, 932, 965
MlyI GAGTC 1 cut(s) 241
MmeI TCCRAC 1 cut(s) 499
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 847
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 4 cut(s) 123, 299, 334, 343
NcoI CCATGG 1 cut(s) 575
NdeII GATC 5 cut(s) 169, 512, 571, 760, 868
NlaIII CATG 5 cut(s) 117, 579, 647, 835, 846
NlaIV GGNNCC 4 cut(s) 243, 573, 623, 917
NmeAIII GCCGAG 1 cut(s) 755
NmuCI GTSAC 1 cut(s) 942
NspI RCATGY 2 cut(s) 647, 835
PaqCI CACCTGC 1 cut(s) 443
PceI AGGCCT 2 cut(s) 293, 478
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 1 cut(s) 839
PkrI GCNGC 3 cut(s) 195, 430, 451
Ple19I CGATCG 2 cut(s) 172, 871
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 4 cut(s) 243, 573, 623, 917
PspOMI GGGCCC 1 cut(s) 915
PspPI GGNCC 4 cut(s) 825, 905, 915, 916
PstI CTGCAG 2 cut(s) 447, 454
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 2 cut(s) 512, 571
PvuI CGATCG 2 cut(s) 172, 871
RsaI GTAC 7 cut(s) 212, 277, 399, 500, 508, 555, 753
RsaNI GTAC 7 cut(s) 211, 276, 398, 499, 507, 554, 752
RseI CAYNNNNRTG 1 cut(s) 847
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 3 cut(s) 194, 429, 450
Sau3AI GATC 5 cut(s) 169, 512, 571, 760, 868
Sau96I GGNCC 4 cut(s) 825, 905, 915, 916
SbfI CCTGCAGG 1 cut(s) 447
ScaI AGTACT 2 cut(s) 277, 753
SchI GAGTC 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 480
SdaI CCTGCAGG 1 cut(s) 447
SduI GDGCHC 1 cut(s) 919
SfaNI GCATC 2 cut(s) 175, 476
SfcI CTRYAG 3 cut(s) 284, 443, 450
SinI GGWCC 2 cut(s) 825, 905
SmiMI CAYNNNNRTG 1 cut(s) 847
Sse8387I CCTGCAGG 1 cut(s) 447
Sse9I AATT 5 cut(s) 21, 94, 662, 932, 965
SseBI AGGCCT 2 cut(s) 293, 478
SsiI CCGC 4 cut(s) 63, 108, 193, 874
StuI AGGCCT 2 cut(s) 293, 478
StyD4I CCNGG 1 cut(s) 478
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 1 cut(s) 503
TaiI ACGT 3 cut(s) 231, 601, 676
TaqI TCGA 5 cut(s) 172, 219, 538, 627, 705
TaqII GACCGA 1 cut(s) 842
TasI AATT 5 cut(s) 21, 94, 662, 932, 965
TatI WGTACW 3 cut(s) 275, 498, 751
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 1 cut(s) 839
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 1 cut(s) 942
TseI GCWGC 2 cut(s) 428, 449
Tsp45I GTSAC 1 cut(s) 942
TspDTI ATGAA 3 cut(s) 383, 655, 831
TspGWI ACGGA 3 cut(s) 21, 254, 717
TspRI CASTG 1 cut(s) 508
VpaK11BI GGWCC 2 cut(s) 825, 905
XapI RAATTY 1 cut(s) 662
XceI RCATGY 2 cut(s) 647, 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 609
XmiI GTMKAC 1 cut(s) 731
ZraI GACGTC 1 cut(s) 229
ZrmI AGTACT 2 cut(s) 277, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.