pycom111g03330

rRNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0009381
Physical Location & Seq
Reverse (-)
2024133 .. 2026718
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03330.3

Sequence Viewer

Length: 1080 bp
ATGAAATTGACGGCTGAGGGAATGCCGCGTGGCGGGAAAGAAAAAGATAACAAAGTGAGAAGCTTTCACTCTACAGTCTACAGCTTGAGTTGGAGCTCTGAGCTCAGGGCGGCAATGGCGGTTTCATCTTCTCCGATCCTCTCTCCATTCCGAATGAAGATAACAGCTACGGAATTTGTCTCACCTTCTTCCACTCTTCCTCGCATCAATTTCTGCAACAATCGACGGCAATCACCTGCCGTCGTCCTCGCTTCCAAGTCGGAGAAAGAGTCGTGGAGTGAGGCCAAGAGAGTGTTGATGGAGCAGTACGGTTTCAACCCCGACGAAGCCTTATCTCCTGAGCCCTCTCCAAACGGAAAGAGGAAGAAAAAGCTGAAAGAGTCTGGACGGGGCGTTGAAATTACACCAAAGGAGCCTAAGGCCGCTCGAACAACCCATAAGTTGCTTACTGTGCTTGGAGGAAAGGCGCGGAGAATGAAGTTGCTAAGTCCAAAAGGTATGGATGTGCGCCCGATGATGGAGGTTGTGAAAGGTGCAGCCTTTGATATCCTGCAGGCTGCTGGTGGTTGTCCTGCATCTTTGAGGCCTGGCCGTTGGTTAGACTTGTACAGTGGTACTGGATCAGTTGGAATTGAAGCTATTAGCCGAGGATGCTCCGAGGTACATTTTGTTGAGATGGATCGATCGGTCGTTTCAGATGTTTTACGTCCAAATTTGGAATGGACTGGGTTTCTTAATATGTCAGTTATACATACTGTTCGTGTTGAAAAATTCATGGAACGTGCACAGAAACTTGCAGGTAAAGAACCATTTGATTACATTAGCGTTACCCCACCGTATATGGAAGTTGACTATGCAGTACTGATGGATCAAATATCAAGTTCAGATCTAGTTGGAGAAGATACCTTTATAGTAGTTGAGTACCCACTAAGGACTGACATGCTGGATTCATGTGGATGCCTTGTGAAGATAACCGATCGACGGTTTGGCAGGACACACTTGGCTATTTATGGACCCAAGTGGGCCCAGAAGAAGAAGAAGAAAGAAAAGTCACTTCGAGGAGCAGCAGTAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95

Protein Analysis

360

Amino Acids

39.92

Weight (kDa)

9.59

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
Acc36I ACCTGC 2 cut(s) 244, 788
AccBSI CCGCTC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 78
AccII CGCG 2 cut(s) 28, 469
AciI CCGC 6 cut(s) 26, 33, 110, 119, 423, 469
AclWI GGATC 4 cut(s) 130, 628, 687, 876
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 4 cut(s) 173, 712, 770, 1074
AfaI GTAC 6 cut(s) 308, 608, 616, 663, 861, 923
AfiI CCNNNNNNNGG 3 cut(s) 32, 517, 981
AgsI TTSAA 4 cut(s) 316, 398, 635, 767
AjnI CCWGG 1 cut(s) 586
AluBI AGCT 7 cut(s) 63, 84, 96, 103, 167, 373, 638
AluI AGCT 7 cut(s) 63, 84, 96, 103, 167, 373, 638
Alw21I GWGCWC 3 cut(s) 98, 105, 787
Alw26I GTCTC 1 cut(s) 184
Alw44I GTGCAC 1 cut(s) 783
AlwI GGATC 4 cut(s) 130, 628, 687, 876
AoxI GGCC 5 cut(s) 282, 420, 584, 589, 1023
ApaI GGGCCC 1 cut(s) 1027
ApaLI GTGCAC 1 cut(s) 783
ApeKI GCWGC 3 cut(s) 536, 557, 1064
ApoI RAATTY 4 cut(s) 173, 712, 770, 1074
AspLEI GCGC 2 cut(s) 469, 510
AspS9I GGNCC 3 cut(s) 1013, 1023, 1024
AsuHPI GGTGA 2 cut(s) 174, 225
AvaII GGWCC 1 cut(s) 1013
AxyI CCTNAGG 1 cut(s) 417
BaeGI GKGCMC 2 cut(s) 787, 1027
BanII GRGCYC 4 cut(s) 98, 105, 345, 1027
Bbv12I GWGCWC 3 cut(s) 98, 105, 787
BbvCI CCTCAGC 1 cut(s) 15
BbvI GCAGC 3 cut(s) 544, 548, 1076
BccI CCATC 4 cut(s) 292, 511, 670, 859
BceAI ACGGC 4 cut(s) 27, 224, 242, 576
BciT130I CCWGG 1 cut(s) 588
BcoDI GTCTC 1 cut(s) 184
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 3 cut(s) 72, 79, 551
BfuAI ACCTGC 2 cut(s) 244, 788
BglII AGATCT 1 cut(s) 886
BisI GCNGC 6 cut(s) 26, 111, 423, 537, 558, 1065
BlsI GCNGC 6 cut(s) 27, 112, 424, 538, 559, 1066
BmcAI AGTACT 1 cut(s) 861
Bme1390I CCNGG 1 cut(s) 588
Bme18I GGWCC 1 cut(s) 1013
BmgT120I GGNCC 3 cut(s) 1013, 1023, 1024
BmiI GGNNCC 3 cut(s) 414, 1015, 1025
BmrFI CCNGG 1 cut(s) 588
BmrI ACTGGG 1 cut(s) 735
BmsI GCATC 4 cut(s) 213, 584, 641, 947
BmuI ACTGGG 1 cut(s) 735
Bpu10I CCTNAGC 3 cut(s) 15, 104, 339
BpuEI CTTGAG 1 cut(s) 106
Bsa29I ATCGAT 1 cut(s) 682
BsaJI CCNNGG 2 cut(s) 646, 657
BsaXI ACNNNNNCTCC 3 cut(s) 254, 284, 1053
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 517, 981
Bse1I ACTGG 2 cut(s) 622, 730
Bse21I CCTNAGG 1 cut(s) 417
Bse3DI GCAATG 1 cut(s) 120
BseBI CCWGG 1 cut(s) 588
BseCI ATCGAT 1 cut(s) 682
BseDI CCNNGG 2 cut(s) 646, 657
BseGI GGATG 3 cut(s) 508, 656, 962
BseLI CCNNNNNNNGG 3 cut(s) 32, 517, 981
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 4 cut(s) 6, 90, 118, 330
BseNI ACTGG 2 cut(s) 622, 730
BseRI GAGGAG 1 cut(s) 1074
BseSI GKGCMC 2 cut(s) 787, 1027
BseXI GCAGC 3 cut(s) 544, 548, 1076
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 2 cut(s) 28, 469
Bsh1285I CGRYCG 3 cut(s) 686, 690, 979
BshFI GGCC 5 cut(s) 284, 422, 586, 591, 1025
BshVI ATCGAT 1 cut(s) 682
BsiEI CGRYCG 3 cut(s) 686, 690, 979
BsiHKAI GWGCWC 3 cut(s) 98, 105, 787
BslI CCNNNNNNNGG 3 cut(s) 32, 517, 981
BsmAI GTCTC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 27
BsnI GGCC 5 cut(s) 284, 422, 586, 591, 1025
Bsp120I GGGCCC 1 cut(s) 1023
Bsp1286I GDGCHC 5 cut(s) 98, 105, 345, 787, 1027
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
BspACI CCGC 6 cut(s) 26, 33, 110, 119, 423, 469
BspANI GGCC 5 cut(s) 284, 422, 586, 591, 1025
BspCNI CTCAG 4 cut(s) 7, 91, 117, 331
BspDI ATCGAT 1 cut(s) 682
BspFNI CGCG 2 cut(s) 28, 469
BspLI GGNNCC 3 cut(s) 414, 1015, 1025
BspMAI CTGCAG 1 cut(s) 555
BspMI ACCTGC 2 cut(s) 244, 788
BspPI GGATC 4 cut(s) 130, 628, 687, 876
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 120
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 2 cut(s) 622, 730
BssECI CCNNGG 2 cut(s) 646, 657
BssMI GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
Bst2UI CCWGG 1 cut(s) 588
Bst4CI ACNGT 7 cut(s) 76, 311, 451, 611, 757, 837, 984
Bst6I CTCTTC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 7 cut(s) 15, 99, 104, 339, 417, 485, 929
BstF5I GGATG 3 cut(s) 508, 656, 962
BstFNI CGCG 2 cut(s) 28, 469
BstHHI GCGC 2 cut(s) 469, 510
BstKTI GATC 7 cut(s) 138, 623, 682, 686, 871, 889, 979
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
BstMCI CGRYCG 3 cut(s) 686, 690, 979
BstMWI GCNNNNNNNGC 3 cut(s) 116, 451, 651
BstNI CCWGG 1 cut(s) 588
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 1 cut(s) 586
BstSFI CTRYAG 3 cut(s) 72, 79, 551
BstSLI GKGCMC 2 cut(s) 787, 1027
BstUI CGCG 2 cut(s) 28, 469
BstV1I GCAGC 3 cut(s) 544, 548, 1076
BstX2I RGATCY 1 cut(s) 886
BstYI RGATCY 1 cut(s) 886
Bsu15I ATCGAT 1 cut(s) 682
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 5 cut(s) 284, 422, 586, 591, 1025
BsuTUI ATCGAT 1 cut(s) 682
BtsCI GGATG 3 cut(s) 508, 656, 962
BtsIMutI CAGTG 1 cut(s) 616
BveI ACCTGC 2 cut(s) 244, 788
Cac8I GCNNGC 1 cut(s) 555
CfoI GCGC 2 cut(s) 469, 510
Cfr13I GGNCC 3 cut(s) 1013, 1023, 1024
ClaI ATCGAT 1 cut(s) 682
Csp6I GTAC 6 cut(s) 307, 607, 615, 662, 860, 922
CviAII CATG 3 cut(s) 775, 940, 951
CviQI GTAC 6 cut(s) 307, 607, 615, 662, 860, 922
DdeI CTNAG 7 cut(s) 15, 99, 104, 339, 417, 485, 929
DpnI GATC 7 cut(s) 137, 622, 681, 685, 870, 888, 978
DpnII GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Ecl136II GAGCTC 2 cut(s) 96, 103
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 4 cut(s) 98, 105, 345, 1027
Eco32I GATATC 1 cut(s) 547
Eco47I GGWCC 1 cut(s) 1013
Eco53kI GAGCTC 2 cut(s) 96, 103
Eco81I CCTNAGG 1 cut(s) 417
EcoICRI GAGCTC 2 cut(s) 96, 103
EcoRII CCWGG 1 cut(s) 586
EcoRV GATATC 1 cut(s) 547
EcoT38I GRGCYC 4 cut(s) 98, 105, 345, 1027
FaeI CATG 3 cut(s) 778, 943, 954
FatI CATG 3 cut(s) 774, 939, 950
FauI CCCGC 1 cut(s) 26
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 6 cut(s) 26, 111, 423, 537, 558, 1065
FokI GGATG 3 cut(s) 515, 663, 969
FriOI GRGCYC 4 cut(s) 98, 105, 345, 1027
Fsp4HI GCNGC 6 cut(s) 26, 111, 423, 537, 558, 1065
FspBI CTAG 1 cut(s) 890
GlaI GCGC 2 cut(s) 468, 509
GluI GCNGC 6 cut(s) 26, 111, 423, 537, 558, 1065
HaeIII GGCC 5 cut(s) 284, 422, 586, 591, 1025
HhaI GCGC 2 cut(s) 469, 510
Hin1II CATG 3 cut(s) 778, 943, 954
Hin6I GCGC 2 cut(s) 467, 508
HinP1I GCGC 2 cut(s) 467, 508
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 3 cut(s) 269, 380, 947
HphI GGTGA 2 cut(s) 174, 225
Hpy166II GTNNAC 3 cut(s) 79, 785, 850
Hpy188I TCNGA 7 cut(s) 100, 135, 152, 262, 658, 697, 886
Hpy188III TCNNGA 2 cut(s) 338, 384
Hpy8I GTNNAC 3 cut(s) 79, 785, 850
Hpy99I CGWCG 4 cut(s) 228, 245, 326, 984
HpyAV CCTTC 1 cut(s) 195
HpyCH4III ACNGT 7 cut(s) 76, 311, 451, 611, 757, 837, 984
HpyCH4IV ACGT 2 cut(s) 706, 781
HpyCH4V TGCA 7 cut(s) 216, 536, 553, 575, 785, 797, 857
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 451, 651
HpyF3I CTNAG 7 cut(s) 15, 99, 104, 339, 417, 485, 929
HpySE526I ACGT 2 cut(s) 706, 781
Hsp92II CATG 3 cut(s) 778, 943, 954
HspAI GCGC 2 cut(s) 467, 508
Kzo9I GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
LmnI GCTCC 5 cut(s) 93, 301, 412, 659, 1061
Lsp1109I GCAGC 3 cut(s) 544, 548, 1076
LweI GCATC 4 cut(s) 213, 584, 641, 947
MaeI CTAG 1 cut(s) 890
MaeII ACGT 2 cut(s) 706, 781
MaeIII GTNAC 2 cut(s) 826, 1050
MalI GATC 7 cut(s) 137, 622, 681, 685, 870, 888, 978
MbiI CCGCTC 1 cut(s) 425
MboI GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
MflI RGATCY 1 cut(s) 886
MhlI GDGCHC 5 cut(s) 98, 105, 345, 787, 1027
MluCI AATT 8 cut(s) 5, 173, 208, 399, 630, 712, 770, 1074
MlyI GAGTC 2 cut(s) 278, 389
MmeI TCCRAC 4 cut(s) 71, 240, 607, 874
MseI TTAA 1 cut(s) 735
MslI CAYNNNNRTG 1 cut(s) 955
MspR9I CCNGG 1 cut(s) 588
Mva1269I GAATGC 1 cut(s) 27
MvaI CCWGG 1 cut(s) 588
MvnI CGCG 2 cut(s) 28, 469
MwoI GCNNNNNNNGC 3 cut(s) 116, 451, 651
NdeII GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
NlaIII CATG 3 cut(s) 778, 943, 954
NlaIV GGNNCC 3 cut(s) 414, 1015, 1025
NmeAIII GCCGAG 1 cut(s) 671
NmuCI GTSAC 1 cut(s) 1050
NspI RCATGY 1 cut(s) 943
PaqCI CACCTGC 1 cut(s) 244
PceI AGGCCT 1 cut(s) 586
PctI GAATGC 1 cut(s) 27
PfeI GAWTC 1 cut(s) 947
PkrI GCNGC 6 cut(s) 27, 112, 424, 538, 559, 1066
Ple19I CGATCG 2 cut(s) 686, 979
PleI GAGTC 2 cut(s) 277, 388
PpsI GAGTC 2 cut(s) 277, 388
Psp124BI GAGCTC 2 cut(s) 98, 105
Psp6I CCWGG 1 cut(s) 586
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 3 cut(s) 414, 1015, 1025
PspOMI GGGCCC 1 cut(s) 1023
PspPI GGNCC 3 cut(s) 1013, 1023, 1024
PstI CTGCAG 1 cut(s) 555
PsuI RGATCY 1 cut(s) 886
PvuI CGATCG 2 cut(s) 686, 979
RsaI GTAC 6 cut(s) 308, 608, 616, 663, 861, 923
RsaNI GTAC 6 cut(s) 307, 607, 615, 662, 860, 922
RseI CAYNNNNRTG 1 cut(s) 955
SacI GAGCTC 2 cut(s) 98, 105
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 6 cut(s) 26, 111, 423, 537, 558, 1065
Sau3AI GATC 7 cut(s) 135, 620, 679, 683, 868, 886, 976
Sau96I GGNCC 3 cut(s) 1013, 1023, 1024
SbfI CCTGCAGG 1 cut(s) 555
ScaI AGTACT 1 cut(s) 861
SchI GAGTC 2 cut(s) 278, 389
ScrFI CCNGG 1 cut(s) 588
SdaI CCTGCAGG 1 cut(s) 555
SduI GDGCHC 5 cut(s) 98, 105, 345, 787, 1027
SfaNI GCATC 4 cut(s) 213, 584, 641, 947
SfcI CTRYAG 3 cut(s) 72, 79, 551
SinI GGWCC 1 cut(s) 1013
SmiMI CAYNNNNRTG 1 cut(s) 955
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
Sse8387I CCTGCAGG 1 cut(s) 555
Sse9I AATT 8 cut(s) 5, 173, 208, 399, 630, 712, 770, 1074
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 6 cut(s) 26, 33, 110, 119, 423, 469
SspMI CTAG 1 cut(s) 890
SstI GAGCTC 2 cut(s) 98, 105
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 1 cut(s) 586
TaaI ACNGT 7 cut(s) 76, 311, 451, 611, 757, 837, 984
TaiI ACGT 2 cut(s) 709, 784
TaqI TCGA 5 cut(s) 223, 427, 682, 979, 1057
TaqII GACCGA 1 cut(s) 676
TasI AATT 8 cut(s) 5, 173, 208, 399, 630, 712, 770, 1074
TatI WGTACW 2 cut(s) 606, 859
TauI GCSGC 3 cut(s) 28, 113, 425
TfiI GAWTC 1 cut(s) 947
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 1 cut(s) 616
TseFI GTSAC 1 cut(s) 1050
TseI GCWGC 3 cut(s) 536, 557, 1064
Tsp45I GTSAC 1 cut(s) 1050
TspDTI ATGAA 6 cut(s) 17, 114, 170, 491, 763, 939
TspGWI ACGGA 2 cut(s) 185, 369
TspRI CASTG 1 cut(s) 616
VneI GTGCAC 1 cut(s) 783
VpaK11BI GGWCC 1 cut(s) 1013
XapI RAATTY 4 cut(s) 173, 712, 770, 1074
XceI RCATGY 1 cut(s) 943
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XmiI GTMKAC 1 cut(s) 78
XspI CTAG 1 cut(s) 890
ZrmI AGTACT 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.