RchiOBHm_Chr2g0169691

rRNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009381
Physical Location & Seq
Forward (+)
83873924 .. 83876637
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53726

Sequence Viewer

Length: 966 bp
ATGGCGCTTTCATCGTCTACAACTCTATCTCCGTTCACTGTGAACATAACCACGGAATTCCTCGGCTCATCTTCAACCTCTCTTCCTCGCTCCAATTTCATCTCCAGACCATGCACCAATCGCCGACCTTCACCTCTCATCGTCCTTTCCTCAAAGTCCGGCGATCGAGCTTGGAACGAGGCCAAGCGACAGTTGATGGAGCAGTACGGCTTCGACCCCGACGTCGCCTTGACTGAGCCGACTCGGAAGGGAAAGAAGAAGAAGAGCAGTACTGAGCTACAGGAGGCCTCACAGGCTGAGCCTAAGCCCAAGAGAACTACGCATAAGTTGCTTCAGGTGCTTGGAGGAAAGGCTCGAAGAATGAAGTTGCTGTCTCCAAAGGGTATGGATGTACGACCAATGATGGAGGTCGTGAAAGGTGCAGCCTTTGATATCCTGCAGGCTGCAGGTGGCTGTCCTGCATCGTTAAGGCCTGGCCGTTGGTTAGACTTGTACAGTGGTACTGGATCTGTTGGAATTGAAGCTATTAGCCGAGGATGTTCTGAGGTGCATTTTGTTGAGATGGATCCGTGGGTGGTTTCAGAGGTTCTACGTCCAAACTTGGAATGGACTGGATTTCTTGATGTTTCAGTTATACATGCTGTGCGTGTTGAAAAGTTCATAGAACGTGCAGAGAAACTTGCTGGTAAAGAACCATTTGATTACATTAGTGTTACCCCTCCGTATACACAAGTTGACTATTCAGTACTGATGGATCAAATATCAAACTCGGCATTAGTTGGAGAAAATACCTTTATAGTGGTTGAGTATGCAACAAGGACCGACATGCTTGATTCATGTGGATGTCTTCTGAAGATAACGGATCGGCGGTTTGGCAGGACACACTTGGCTATTTATGGACCCAAGTGGGCCCAGAAGAAGAAGAAGTTAGAAAAGTCACTACGGGGAGTAGCAGTAGATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95

Protein Analysis

321

Amino Acids

35.6

Weight (kDa)

9.41

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cons_hypoth95 PF03602 111 - 299 7.9e-46 Conserved hypothetical protein 95
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 437
AasI GACNNNNNNGTC 1 cut(s) 221
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 437
AccI GTMKAC 2 cut(s) 17, 725
AciI CCGC 1 cut(s) 868
AclWI GGATC 5 cut(s) 514, 560, 573, 762, 870
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 1 cut(s) 56
AcuI CTGAAG 2 cut(s) 317, 872
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 6 cut(s) 206, 271, 393, 494, 502, 747
AgsI TTSAA 3 cut(s) 75, 521, 653
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 3 cut(s) 170, 277, 524
AluI AGCT 3 cut(s) 170, 277, 524
Alw26I GTCTC 1 cut(s) 378
AlwI GGATC 5 cut(s) 514, 560, 573, 762, 870
AoxI GGCC 5 cut(s) 180, 285, 470, 475, 909
ApaI GGGCCC 1 cut(s) 913
ApeKI GCWGC 2 cut(s) 422, 443
ApoI RAATTY 1 cut(s) 56
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 4 cut(s) 819, 899, 909, 910
AsuHPI GGTGA 1 cut(s) 123
AvaII GGWCC 2 cut(s) 819, 899
BaeGI GKGCMC 1 cut(s) 913
BamHI GGATCC 1 cut(s) 565
BanII GRGCYC 1 cut(s) 913
BbsI GAAGAC 1 cut(s) 839
BbvI GCAGC 2 cut(s) 430, 434
BccI CCATC 4 cut(s) 190, 397, 556, 745
BceAI ACGGC 2 cut(s) 223, 462
BciT130I CCWGG 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 378
BfmI CTRYAG 4 cut(s) 278, 437, 444, 962
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 437
BglI GCCNNNNNGGC 1 cut(s) 293
BglII AGATCT 1 cut(s) 958
BisI GCNGC 2 cut(s) 423, 444
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 2 cut(s) 424, 445
BmcAI AGTACT 2 cut(s) 271, 747
Bme1390I CCNGG 1 cut(s) 474
Bme18I GGWCC 2 cut(s) 819, 899
BmgT120I GGNCC 4 cut(s) 819, 899, 909, 910
BmiI GGNNCC 3 cut(s) 567, 901, 911
BmrFI CCNGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 839
BpmI CTGGAG 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 303
Bpu1102I GCTNAGC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 4 cut(s) 51, 61, 532, 569
BsaXI ACNNNNNCTCC 3 cut(s) 13, 43, 939
Bse1I ACTGG 2 cut(s) 508, 616
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 4 cut(s) 51, 61, 532, 569
BseGI GGATG 3 cut(s) 394, 542, 848
BseMII CTCAG 4 cut(s) 225, 264, 288, 534
BseNI ACTGG 2 cut(s) 508, 616
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 2 cut(s) 430, 434
BsgI GTGCAG 2 cut(s) 441, 690
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 5 cut(s) 182, 287, 472, 477, 911
BsiEI CGRYCG 1 cut(s) 166
BsiSI CCGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 378
BsnI GGCC 5 cut(s) 182, 287, 472, 477, 911
Bsp120I GGGCCC 1 cut(s) 909
Bsp1286I GDGCHC 1 cut(s) 913
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 6 cut(s) 163, 506, 565, 754, 862, 958
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 1 cut(s) 868
BspANI GGCC 5 cut(s) 182, 287, 472, 477, 911
BspCNI CTCAG 4 cut(s) 226, 265, 289, 535
BspLI GGNNCC 3 cut(s) 567, 901, 911
BspMAI CTGCAG 2 cut(s) 441, 448
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 5 cut(s) 514, 560, 573, 762, 870
BspQI GCTCTTC 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 508, 616
BssECI CCNNGG 4 cut(s) 51, 61, 532, 569
BssMI GATC 6 cut(s) 163, 506, 565, 754, 862, 958
BssNAI GTATAC 1 cut(s) 726
BssNI GRCGYC 1 cut(s) 222
Bst1107I GTATAC 1 cut(s) 726
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 3 cut(s) 40, 192, 497
Bst6I CTCTTC 2 cut(s) 87, 257
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 5 cut(s) 234, 273, 297, 303, 543
BstDSI CCRYGG 2 cut(s) 51, 569
BstF5I GGATG 3 cut(s) 394, 542, 848
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 6 cut(s) 166, 509, 568, 757, 865, 961
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 6 cut(s) 163, 506, 565, 754, 862, 958
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 4 cut(s) 120, 293, 328, 337
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 2 cut(s) 641, 829
BstSCI CCNGG 1 cut(s) 472
BstSFI CTRYAG 4 cut(s) 278, 437, 444, 962
BstSLI GKGCMC 1 cut(s) 913
BstV1I GCAGC 2 cut(s) 430, 434
BstV2I GAAGAC 1 cut(s) 839
BstX2I RGATCY 3 cut(s) 506, 565, 958
BstYI RGATCY 3 cut(s) 506, 565, 958
BstZ17I GTATAC 1 cut(s) 726
BsuRI GGCC 5 cut(s) 182, 287, 472, 477, 911
BtgI CCRYGG 2 cut(s) 51, 569
BtsCI GGATG 3 cut(s) 394, 542, 848
BtsIMutI CAGTG 2 cut(s) 36, 502
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 1 cut(s) 441
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 819, 899, 909, 910
Csp6I GTAC 6 cut(s) 205, 270, 392, 493, 501, 746
CviAII CATG 4 cut(s) 111, 638, 826, 837
CviQI GTAC 6 cut(s) 205, 270, 392, 493, 501, 746
DdeI CTNAG 5 cut(s) 234, 273, 297, 303, 543
DpnI GATC 6 cut(s) 165, 508, 567, 756, 864, 960
DpnII GATC 6 cut(s) 163, 506, 565, 754, 862, 958
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 2 cut(s) 87, 257
EarI CTCTTC 2 cut(s) 87, 257
Eco147I AGGCCT 2 cut(s) 287, 472
Eco24I GRGCYC 1 cut(s) 913
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 2 cut(s) 819, 899
Eco57I CTGAAG 2 cut(s) 317, 872
EcoRI GAATTC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 472
EcoRV GATATC 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 913
FaeI CATG 4 cut(s) 114, 641, 829, 840
FatI CATG 4 cut(s) 110, 637, 825, 836
FblI GTMKAC 2 cut(s) 17, 725
Fnu4HI GCNGC 2 cut(s) 423, 444
FokI GGATG 3 cut(s) 401, 549, 855
FriOI GRGCYC 1 cut(s) 913
Fsp4HI GCNGC 2 cut(s) 423, 444
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 423, 444
GsuI CTGGAG 1 cut(s) 88
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 182, 287, 472, 477, 911
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 114, 641, 829, 840
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 2 cut(s) 241, 833
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 123
Hpy166II GTNNAC 5 cut(s) 18, 36, 43, 726, 736
Hpy188I TCNGA 4 cut(s) 246, 544, 583, 852
Hpy188III TCNNGA 3 cut(s) 105, 412, 620
Hpy8I GTNNAC 5 cut(s) 18, 36, 43, 726, 736
Hpy99I CGWCG 2 cut(s) 224, 227
HpyAV CCTTC 2 cut(s) 138, 241
HpyCH4III ACNGT 3 cut(s) 40, 192, 497
HpyCH4IV ACGT 3 cut(s) 222, 592, 667
HpyCH4V TGCA 8 cut(s) 114, 422, 439, 446, 461, 550, 671, 812
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 293, 328, 337
HpyF3I CTNAG 5 cut(s) 234, 273, 297, 303, 543
HpySE526I ACGT 3 cut(s) 222, 592, 667
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 114, 641, 829, 840
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 6 cut(s) 163, 506, 565, 754, 862, 958
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 2 cut(s) 95, 199
Lsp1109I GCAGC 2 cut(s) 430, 434
LweI GCATC 1 cut(s) 470
MaeII ACGT 3 cut(s) 222, 592, 667
MaeIII GTNAC 2 cut(s) 712, 936
MalI GATC 6 cut(s) 165, 508, 567, 756, 864, 960
MboI GATC 6 cut(s) 163, 506, 565, 754, 862, 958
MflI RGATCY 3 cut(s) 506, 565, 958
MhlI GDGCHC 1 cut(s) 913
MluCI AATT 3 cut(s) 56, 94, 516
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 2 cut(s) 493, 760
MseI TTAA 1 cut(s) 467
MslI CAYNNNNRTG 1 cut(s) 841
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 474
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 4 cut(s) 120, 293, 328, 337
NdeII GATC 6 cut(s) 163, 506, 565, 754, 862, 958
NlaIII CATG 4 cut(s) 114, 641, 829, 840
NlaIV GGNNCC 3 cut(s) 567, 901, 911
NmeAIII GCCGAG 3 cut(s) 42, 557, 749
NmuCI GTSAC 1 cut(s) 936
NspI RCATGY 2 cut(s) 641, 829
PaqCI CACCTGC 1 cut(s) 437
PceI AGGCCT 2 cut(s) 287, 472
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 1 cut(s) 833
PkrI GCNGC 2 cut(s) 424, 445
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 3 cut(s) 567, 901, 911
PspOMI GGGCCC 1 cut(s) 909
PspPI GGNCC 4 cut(s) 819, 899, 909, 910
PstI CTGCAG 2 cut(s) 441, 448
PsuI RGATCY 3 cut(s) 506, 565, 958
PvuI CGATCG 1 cut(s) 166
RsaI GTAC 6 cut(s) 206, 271, 393, 494, 502, 747
RsaNI GTAC 6 cut(s) 205, 270, 392, 493, 501, 746
RseI CAYNNNNRTG 1 cut(s) 841
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 1 cut(s) 467
SatI GCNGC 2 cut(s) 423, 444
Sau3AI GATC 6 cut(s) 163, 506, 565, 754, 862, 958
Sau96I GGNCC 4 cut(s) 819, 899, 909, 910
SbfI CCTGCAGG 1 cut(s) 441
ScaI AGTACT 2 cut(s) 271, 747
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 474
SdaI CCTGCAGG 1 cut(s) 441
SduI GDGCHC 1 cut(s) 913
SfaNI GCATC 1 cut(s) 470
SfcI CTRYAG 4 cut(s) 278, 437, 444, 962
SinI GGWCC 2 cut(s) 819, 899
SmiMI CAYNNNNRTG 1 cut(s) 841
Sse8387I CCTGCAGG 1 cut(s) 441
Sse9I AATT 3 cut(s) 56, 94, 516
SseBI AGGCCT 2 cut(s) 287, 472
SsiI CCGC 1 cut(s) 868
StuI AGGCCT 2 cut(s) 287, 472
StyD4I CCNGG 1 cut(s) 472
TaaI ACNGT 3 cut(s) 40, 192, 497
TaiI ACGT 3 cut(s) 225, 595, 670
TaqI TCGA 3 cut(s) 166, 213, 355
TaqII GACCGA 1 cut(s) 836
TasI AATT 3 cut(s) 56, 94, 516
TatI WGTACW 3 cut(s) 269, 492, 745
TfiI GAWTC 1 cut(s) 833
Tru1I TTAA 1 cut(s) 467
Tru9I TTAA 1 cut(s) 467
TscAI CASTG 2 cut(s) 43, 502
TseFI GTSAC 1 cut(s) 936
TseI GCWGC 2 cut(s) 422, 443
Tsp45I GTSAC 1 cut(s) 936
TspDTI ATGAA 4 cut(s) 88, 377, 649, 825
TspGWI ACGGA 5 cut(s) 21, 68, 558, 711, 875
TspRI CASTG 2 cut(s) 43, 502
VpaK11BI GGWCC 2 cut(s) 819, 899
XapI RAATTY 1 cut(s) 56
XceI RCATGY 2 cut(s) 641, 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 603
XmiI GTMKAC 2 cut(s) 17, 725
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 2 cut(s) 271, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.