Rw2G050940

rRNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009381
Physical Location & Seq
Forward (+)
78639037 .. 78641205
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050940.1

Sequence Viewer

Length: 951 bp
ATGGCGCTTCTATCTCCGTTCACTGTGAACGTAACCACGGAATTCCTCGGCTCATCTTCAACCTCTCTTCCTCGCTCCAATTTCATCTCCAGACTATGCACCAATCGCCGACCTTCACCTCTCATCGTCCTTTCCTCAAAGTCCGGCGATCGAGCTTGGAACGAGGCCAAGCGACAGTTGATGGAGCAGTACGGCTTCGACCCCGACGTCGCCTTGACTGAGCCGACTCGGAAGGGAAAGAAGAAGAAGAGCAGTACTGAGCTACAGGAGGCCTCACAGGCTGAGCCTAAGCCCAAGAGAACTACGCATAAGTTGCTTCAGGTGCTTGGAGGAAAGGCTCGAAGAATGAAGTTGCTGTCTCCAAAGGGTATGGATGTACGACCAATGATGGAGGTCGTGAAAGGTGCAGCCTTTGATATCCTGCAGGCTGCAGGTGGCTGTCCTGCATCGTTAAGGCCTGGCCGTTGGTTGGACTTGTACAGTGGTACTGGATCTGTTGGAATTGAAGCTATTAGCCGAGGATGTTCTGAGGTGCATTTTGTTGAGATGGATCCGTGGGTGGTTTCAGAGGTTCTACGTCCAAACTTGGAATGGACTGGATTTCTTGATGTTTCAGTTATACATGCTGTGCGTGTTGAAAAGTTCATAGAACGTGCAGAGAAACTTGCTGGTAAAGAACCATTTGATTACATTAGTGTTACCCCTCCGTATACACAAGTTGACTATTCAGTACTGATGGATCAAATATCAAACTCGGCATTAGTTGGAGAAAATACCTTTATAGTGGTTGAGTATGCAACAAGGACCGACATGCTTGATTCATGTGGATGTCTTCTGAAGATAACCGATCGGCGGTTTGGCAGGACACACTTGGCTATTTATGGACCCAAGTGGGCCCAGAAGAAGAAGAAGTTAGAAAAGTCACTACGGGGAGTAGCAGTAGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95

Protein Analysis

316

Amino Acids

35.15

Weight (kDa)

9.41

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cons_hypoth95 PF03602 106 - 294 7.6e-46 Conserved hypothetical protein 95
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 422
AasI GACNNNNNNGTC 1 cut(s) 206
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 422
AccI GTMKAC 1 cut(s) 710
AciI CCGC 1 cut(s) 853
AclWI GGATC 4 cut(s) 499, 545, 558, 747
AcoI YGGCCR 1 cut(s) 460
AcsI RAATTY 1 cut(s) 41
AcuI CTGAAG 2 cut(s) 302, 857
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 6 cut(s) 191, 256, 378, 479, 487, 732
AfiI CCNNNNNNNGG 2 cut(s) 469, 852
AgsI TTSAA 3 cut(s) 60, 506, 638
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 3 cut(s) 155, 262, 509
AluI AGCT 3 cut(s) 155, 262, 509
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 4 cut(s) 499, 545, 558, 747
AoxI GGCC 5 cut(s) 165, 270, 455, 460, 894
ApaI GGGCCC 1 cut(s) 898
ApeKI GCWGC 2 cut(s) 407, 428
ApoI RAATTY 1 cut(s) 41
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 4 cut(s) 804, 884, 894, 895
AsuHPI GGTGA 1 cut(s) 108
AvaII GGWCC 2 cut(s) 804, 884
BaeGI GKGCMC 1 cut(s) 898
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 898
BbsI GAAGAC 1 cut(s) 824
BbvI GCAGC 2 cut(s) 415, 419
BccI CCATC 4 cut(s) 175, 382, 541, 730
BceAI ACGGC 2 cut(s) 208, 447
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 363
BfmI CTRYAG 4 cut(s) 263, 422, 429, 947
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 278
BisI GCNGC 2 cut(s) 408, 429
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 2 cut(s) 409, 430
BmcAI AGTACT 2 cut(s) 256, 732
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 2 cut(s) 804, 884
BmgT120I GGNCC 4 cut(s) 804, 884, 894, 895
BmiI GGNNCC 3 cut(s) 552, 886, 896
BmrFI CCNGG 1 cut(s) 459
BmsI GCATC 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 824
BpmI CTGGAG 1 cut(s) 73
Bpu10I CCTNAGC 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 4 cut(s) 36, 46, 517, 554
BsaXI ACNNNNNCTCC 1 cut(s) 924
Bsc4I CCNNNNNNNGG 2 cut(s) 469, 852
Bse1I ACTGG 2 cut(s) 493, 601
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 4 cut(s) 36, 46, 517, 554
BseGI GGATG 3 cut(s) 379, 527, 833
BseLI CCNNNNNNNGG 2 cut(s) 469, 852
BseMII CTCAG 4 cut(s) 210, 249, 273, 519
BseNI ACTGG 2 cut(s) 493, 601
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 2 cut(s) 415, 419
BsgI GTGCAG 2 cut(s) 426, 675
Bsh1285I CGRYCG 2 cut(s) 151, 850
BshFI GGCC 5 cut(s) 167, 272, 457, 462, 896
BsiEI CGRYCG 2 cut(s) 151, 850
BsiSI CCGG 1 cut(s) 144
BslI CCNNNNNNNGG 2 cut(s) 469, 852
BsmAI GTCTC 1 cut(s) 363
BsnI GGCC 5 cut(s) 167, 272, 457, 462, 896
Bsp120I GGGCCC 1 cut(s) 894
Bsp1286I GDGCHC 1 cut(s) 898
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 5 cut(s) 148, 491, 550, 739, 847
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 1 cut(s) 853
BspANI GGCC 5 cut(s) 167, 272, 457, 462, 896
BspCNI CTCAG 4 cut(s) 211, 250, 274, 520
BspLI GGNNCC 3 cut(s) 552, 886, 896
BspMAI CTGCAG 2 cut(s) 426, 433
BspMI ACCTGC 1 cut(s) 422
BspPI GGATC 4 cut(s) 499, 545, 558, 747
BspQI GCTCTTC 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 2 cut(s) 493, 601
BssECI CCNNGG 4 cut(s) 36, 46, 517, 554
BssMI GATC 5 cut(s) 148, 491, 550, 739, 847
BssNAI GTATAC 1 cut(s) 711
BssNI GRCGYC 1 cut(s) 207
Bst1107I GTATAC 1 cut(s) 711
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 4 cut(s) 25, 177, 482, 948
Bst6I CTCTTC 2 cut(s) 72, 242
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 313
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 5 cut(s) 219, 258, 282, 288, 528
BstDSI CCRYGG 2 cut(s) 36, 554
BstF5I GGATG 3 cut(s) 379, 527, 833
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 151, 494, 553, 742, 850
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 5 cut(s) 148, 491, 550, 739, 847
BstMCI CGRYCG 2 cut(s) 151, 850
BstMWI GCNNNNNNNGC 4 cut(s) 105, 278, 313, 322
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 2 cut(s) 626, 814
BstSCI CCNGG 1 cut(s) 457
BstSFI CTRYAG 4 cut(s) 263, 422, 429, 947
BstSLI GKGCMC 1 cut(s) 898
BstV1I GCAGC 2 cut(s) 415, 419
BstV2I GAAGAC 1 cut(s) 824
BstX2I RGATCY 2 cut(s) 491, 550
BstYI RGATCY 2 cut(s) 491, 550
BstZ17I GTATAC 1 cut(s) 711
BsuRI GGCC 5 cut(s) 167, 272, 457, 462, 896
BtgI CCRYGG 2 cut(s) 36, 554
BtsCI GGATG 3 cut(s) 379, 527, 833
BtsIMutI CAGTG 2 cut(s) 21, 487
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 804, 884, 894, 895
Csp6I GTAC 6 cut(s) 190, 255, 377, 478, 486, 731
CviAII CATG 3 cut(s) 623, 811, 822
CviQI GTAC 6 cut(s) 190, 255, 377, 478, 486, 731
DdeI CTNAG 5 cut(s) 219, 258, 282, 288, 528
DpnI GATC 5 cut(s) 150, 493, 552, 741, 849
DpnII GATC 5 cut(s) 148, 491, 550, 739, 847
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 460
Eam1104I CTCTTC 2 cut(s) 72, 242
EarI CTCTTC 2 cut(s) 72, 242
Eco147I AGGCCT 2 cut(s) 272, 457
Eco24I GRGCYC 1 cut(s) 898
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 804, 884
Eco57I CTGAAG 2 cut(s) 302, 857
EcoRI GAATTC 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 457
EcoRV GATATC 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 898
FaeI CATG 3 cut(s) 626, 814, 825
FatI CATG 3 cut(s) 622, 810, 821
FblI GTMKAC 1 cut(s) 710
Fnu4HI GCNGC 2 cut(s) 408, 429
FokI GGATG 3 cut(s) 386, 534, 840
FriOI GRGCYC 1 cut(s) 898
Fsp4HI GCNGC 2 cut(s) 408, 429
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 408, 429
GsuI CTGGAG 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 167, 272, 457, 462, 896
HapII CCGG 1 cut(s) 144
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 3 cut(s) 626, 814, 825
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HinfI GANTC 2 cut(s) 226, 818
HpaII CCGG 1 cut(s) 144
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 4 cut(s) 21, 28, 711, 721
Hpy188I TCNGA 4 cut(s) 231, 529, 568, 837
Hpy188III TCNNGA 3 cut(s) 90, 397, 605
Hpy8I GTNNAC 4 cut(s) 21, 28, 711, 721
Hpy99I CGWCG 2 cut(s) 209, 212
HpyAV CCTTC 2 cut(s) 123, 226
HpyCH4III ACNGT 4 cut(s) 25, 177, 482, 948
HpyCH4IV ACGT 4 cut(s) 30, 207, 577, 652
HpyCH4V TGCA 8 cut(s) 99, 407, 424, 431, 446, 535, 656, 797
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 278, 313, 322
HpyF3I CTNAG 5 cut(s) 219, 258, 282, 288, 528
HpySE526I ACGT 4 cut(s) 30, 207, 577, 652
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 3 cut(s) 626, 814, 825
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 5 cut(s) 148, 491, 550, 739, 847
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 2 cut(s) 80, 184
Lsp1109I GCAGC 2 cut(s) 415, 419
LweI GCATC 1 cut(s) 455
MaeII ACGT 4 cut(s) 30, 207, 577, 652
MaeIII GTNAC 3 cut(s) 31, 697, 921
MalI GATC 5 cut(s) 150, 493, 552, 741, 849
MboI GATC 5 cut(s) 148, 491, 550, 739, 847
MflI RGATCY 2 cut(s) 491, 550
MhlI GDGCHC 1 cut(s) 898
MluCI AATT 3 cut(s) 41, 79, 501
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 3 cut(s) 450, 478, 745
MseI TTAA 1 cut(s) 452
MslI CAYNNNNRTG 1 cut(s) 826
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 4 cut(s) 105, 278, 313, 322
NdeII GATC 5 cut(s) 148, 491, 550, 739, 847
NlaIII CATG 3 cut(s) 626, 814, 825
NlaIV GGNNCC 3 cut(s) 552, 886, 896
NmeAIII GCCGAG 3 cut(s) 27, 542, 734
NmuCI GTSAC 1 cut(s) 921
NspI RCATGY 2 cut(s) 626, 814
PaqCI CACCTGC 1 cut(s) 422
PceI AGGCCT 2 cut(s) 272, 457
PciSI GCTCTTC 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 1 cut(s) 818
PkrI GCNGC 2 cut(s) 409, 430
Ple19I CGATCG 2 cut(s) 151, 850
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 3 cut(s) 552, 886, 896
PspOMI GGGCCC 1 cut(s) 894
PspPI GGNCC 4 cut(s) 804, 884, 894, 895
PstI CTGCAG 2 cut(s) 426, 433
PsuI RGATCY 2 cut(s) 491, 550
PvuI CGATCG 2 cut(s) 151, 850
RsaI GTAC 6 cut(s) 191, 256, 378, 479, 487, 732
RsaNI GTAC 6 cut(s) 190, 255, 377, 478, 486, 731
RseI CAYNNNNRTG 1 cut(s) 826
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 2 cut(s) 408, 429
Sau3AI GATC 5 cut(s) 148, 491, 550, 739, 847
Sau96I GGNCC 4 cut(s) 804, 884, 894, 895
SbfI CCTGCAGG 1 cut(s) 426
ScaI AGTACT 2 cut(s) 256, 732
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 459
SdaI CCTGCAGG 1 cut(s) 426
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 1 cut(s) 455
SfcI CTRYAG 4 cut(s) 263, 422, 429, 947
SinI GGWCC 2 cut(s) 804, 884
SmiMI CAYNNNNRTG 1 cut(s) 826
Sse8387I CCTGCAGG 1 cut(s) 426
Sse9I AATT 3 cut(s) 41, 79, 501
SseBI AGGCCT 2 cut(s) 272, 457
SsiI CCGC 1 cut(s) 853
StuI AGGCCT 2 cut(s) 272, 457
StyD4I CCNGG 1 cut(s) 457
TaaI ACNGT 4 cut(s) 25, 177, 482, 948
TaiI ACGT 4 cut(s) 33, 210, 580, 655
TaqI TCGA 3 cut(s) 151, 198, 340
TaqII GACCGA 1 cut(s) 821
TasI AATT 3 cut(s) 41, 79, 501
TatI WGTACW 3 cut(s) 254, 477, 730
TfiI GAWTC 1 cut(s) 818
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TscAI CASTG 2 cut(s) 28, 487
TseFI GTSAC 1 cut(s) 921
TseI GCWGC 2 cut(s) 407, 428
Tsp45I GTSAC 1 cut(s) 921
TspDTI ATGAA 4 cut(s) 73, 362, 634, 810
TspGWI ACGGA 4 cut(s) 6, 53, 543, 696
TspRI CASTG 2 cut(s) 28, 487
VpaK11BI GGWCC 2 cut(s) 804, 884
XapI RAATTY 1 cut(s) 41
XceI RCATGY 2 cut(s) 626, 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 588
XmiI GTMKAC 1 cut(s) 710
ZraI GACGTC 1 cut(s) 208
ZrmI AGTACT 2 cut(s) 256, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.