Rroxscaffold_2G00081880

rRNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009381
Physical Location & Seq
Reverse (-)
4838885 .. 4844126
5242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081880.1

Sequence Viewer

Length: 1146 bp
ATGGCGCTTTCATTGTCTACAACTCCATCTCCGTTCACTGTGAACGTAACTACAGACTTCCTCGGCTCATCTTCAACCTCTCTTCCTCGCTTCAATTTCATCTCCAGACCATGCACCAATCGCCGACCTTCACCTCTCATCGTCCTTTCCTCAAAGTCCGGCGATCGAGCTTGGAACGAGGCCAAGCGACAGTTGATGGAGCAGTACGGCTTCGACCCCGACGTCGCCTTGACTGAGCGGACTCGGAAGGGAAAGAAGAAGAAGAGCAGTACTGAGCTACAGGAGGCCTCACAGGCTGAGCCTAAGCCCAAGAGAACTACGCATAAGTTGCTTCAGGTGCTTGGAGGAAAGGCTCGAAGAATGAAGTTGCTGTCTCCAAAGGGAATGGATGTACGACCAATGATGGAGGTCGTGAAAGGTGCAGCCTTTGATATCCTGCAGGCTGCAGGTGGCTGTCCTGCATCTTTAAGGCCTGGCCGTTGGTTAGACTTGTACAGTGGTACTGGATCTGTTGGAATTGAAGCTATTAGCCGAGGATGTTCTGAGGAAACTTACGTATGTAAGAAATTCCAGGTGCATTTTGTTGAGATGGATCCGTGGGTGGTTTCAGAGGTTCTACGTCCAAACTTGGAATGGACTGGATTTCTTGATGTTTCAGTTATACATCCTGTGCGTGTTGAAAAGTTCATAGAACGTGCAGAGAAACTTGCTGGTAAAGAACCATTTGATTACATTAGTGTTACCCCTCCGTATACACAAGTTGACTATGCAGTACTGATGGATCAAATATCAAACTCGGCATTAGTTGGAGAAAATACCTTTATAGTGGTTGAGTATGCAACAAGGACCGACATGCTTGATTCATGTGGATGTCTTCTGAAGATAACCGATCGGCGGTTTGGCAGGACACACTTGGCTATTTATGGACCCAAGTGGGCCCAGAAGAAGAAGAAGTTAGAAAAGTCAATACGGGGAGTAGCAGCTCGCAGCAGCTCTACTGCATGGATTGGTCTTACTTCCTTTAAGGTTGGAGTGGAGCACAGAATTAACACTGGACCTCTTAAAAGCACAATTTCTTTTGTGTTTGGGATTGGAGATTATATGTTTGAAGTTCTAGATAGAAATAGTGACTGGACTATGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95

Protein Analysis

381

Amino Acids

42.54

Weight (kDa)

9.41

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cons_hypoth95 PF03602 111 - 308 1.3e-40 Conserved hypothetical protein 95
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 437
AasI GACNNNNNNGTC 1 cut(s) 221
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 238
AccI GTMKAC 2 cut(s) 17, 752
AciI CCGC 2 cut(s) 238, 895
AclWI GGATC 4 cut(s) 514, 587, 600, 789
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 1 cut(s) 566
AcuI CTGAAG 2 cut(s) 317, 899
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 6 cut(s) 206, 271, 393, 494, 502, 774
AfiI CCNNNNNNNGG 1 cut(s) 894
AgsI TTSAA 5 cut(s) 75, 94, 521, 680, 1109
AjnI CCWGG 2 cut(s) 472, 570
AluBI AGCT 5 cut(s) 170, 277, 524, 983, 993
AluI AGCT 5 cut(s) 170, 277, 524, 983, 993
Alw21I GWGCWC 1 cut(s) 1041
Alw26I GTCTC 1 cut(s) 378
AlwI GGATC 4 cut(s) 514, 587, 600, 789
AoxI GGCC 5 cut(s) 180, 285, 470, 475, 936
ApaI GGGCCC 1 cut(s) 940
ApeKI GCWGC 5 cut(s) 422, 443, 980, 987, 990
ApoI RAATTY 1 cut(s) 566
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 5 cut(s) 846, 926, 936, 937, 1055
AsuHPI GGTGA 1 cut(s) 123
AvaII GGWCC 3 cut(s) 846, 926, 1055
BaeGI GKGCMC 1 cut(s) 940
BamHI GGATCC 1 cut(s) 592
BanII GRGCYC 1 cut(s) 940
BbsI GAAGAC 1 cut(s) 866
Bbv12I GWGCWC 1 cut(s) 1041
BbvI GCAGC 5 cut(s) 430, 434, 992, 999, 1002
BccI CCATC 5 cut(s) 34, 190, 397, 583, 772
BceAI ACGGC 2 cut(s) 223, 462
BciT130I CCWGG 2 cut(s) 474, 572
BcoDI GTCTC 1 cut(s) 378
BfaI CTAG 1 cut(s) 1115
BfmI CTRYAG 4 cut(s) 51, 278, 437, 444
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 437
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 5 cut(s) 423, 444, 981, 988, 991
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 5 cut(s) 424, 445, 982, 989, 992
BmcAI AGTACT 2 cut(s) 271, 774
Bme1390I CCNGG 2 cut(s) 474, 572
Bme18I GGWCC 3 cut(s) 846, 926, 1055
BmgT120I GGNCC 5 cut(s) 846, 926, 936, 937, 1055
BmiI GGNNCC 3 cut(s) 594, 928, 938
BmrFI CCNGG 2 cut(s) 474, 572
BmsI GCATC 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 866
BpmI CTGGAG 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 303
Bpu1102I GCTNAGC 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 556
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 61, 532, 596
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 894
Bse1I ACTGG 4 cut(s) 508, 643, 1057, 1136
BseBI CCWGG 2 cut(s) 474, 572
BseDI CCNNGG 3 cut(s) 61, 532, 596
BseGI GGATG 4 cut(s) 394, 542, 664, 875
BseLI CCNNNNNNNGG 1 cut(s) 894
BseMII CTCAG 4 cut(s) 225, 264, 288, 534
BseNI ACTGG 4 cut(s) 508, 643, 1057, 1136
BseSI GKGCMC 1 cut(s) 940
BseXI GCAGC 5 cut(s) 430, 434, 992, 999, 1002
BsgI GTGCAG 2 cut(s) 441, 717
Bsh1285I CGRYCG 2 cut(s) 166, 892
BshFI GGCC 5 cut(s) 182, 287, 472, 477, 938
BsiEI CGRYCG 2 cut(s) 166, 892
BsiHKAI GWGCWC 1 cut(s) 1041
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 894
BsmAI GTCTC 1 cut(s) 378
BsnI GGCC 5 cut(s) 182, 287, 472, 477, 938
Bsp120I GGGCCC 1 cut(s) 936
Bsp1286I GDGCHC 2 cut(s) 940, 1041
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 5 cut(s) 163, 506, 592, 781, 889
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 2 cut(s) 238, 895
BspANI GGCC 5 cut(s) 182, 287, 472, 477, 938
BspCNI CTCAG 4 cut(s) 226, 265, 289, 535
BspLI GGNNCC 3 cut(s) 594, 928, 938
BspMAI CTGCAG 2 cut(s) 441, 448
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 4 cut(s) 514, 587, 600, 789
BspQI GCTCTTC 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 4 cut(s) 508, 643, 1057, 1136
BssECI CCNNGG 3 cut(s) 61, 532, 596
BssMI GATC 5 cut(s) 163, 506, 592, 781, 889
BssNAI GTATAC 1 cut(s) 753
BssNI GRCGYC 1 cut(s) 222
Bst1107I GTATAC 1 cut(s) 753
Bst2UI CCWGG 2 cut(s) 474, 572
Bst4CI ACNGT 3 cut(s) 40, 192, 497
Bst6I CTCTTC 2 cut(s) 87, 257
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 328
BstAUI TGTACA 1 cut(s) 492
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 2 cut(s) 441, 985
BstDEI CTNAG 5 cut(s) 234, 273, 297, 303, 543
BstDSI CCRYGG 1 cut(s) 596
BstF5I GGATG 4 cut(s) 394, 542, 664, 875
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 5 cut(s) 166, 509, 595, 784, 892
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 5 cut(s) 163, 506, 592, 781, 889
BstMCI CGRYCG 2 cut(s) 166, 892
BstMWI GCNNNNNNNGC 4 cut(s) 120, 293, 328, 337
BstNI CCWGG 2 cut(s) 474, 572
BstNSI RCATGY 1 cut(s) 856
BstSCI CCNGG 2 cut(s) 472, 570
BstSFI CTRYAG 4 cut(s) 51, 278, 437, 444
BstSLI GKGCMC 1 cut(s) 940
BstSNI TACGTA 1 cut(s) 556
BstV1I GCAGC 5 cut(s) 430, 434, 992, 999, 1002
BstV2I GAAGAC 1 cut(s) 866
BstX2I RGATCY 2 cut(s) 506, 592
BstYI RGATCY 2 cut(s) 506, 592
BstZ17I GTATAC 1 cut(s) 753
BsuRI GGCC 5 cut(s) 182, 287, 472, 477, 938
BtgI CCRYGG 1 cut(s) 596
BtsCI GGATG 4 cut(s) 394, 542, 664, 875
BtsIMutI CAGTG 3 cut(s) 36, 502, 1050
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 2 cut(s) 441, 985
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 5 cut(s) 846, 926, 936, 937, 1055
Csp6I GTAC 6 cut(s) 205, 270, 392, 493, 501, 773
CviAII CATG 4 cut(s) 111, 853, 864, 1002
CviQI GTAC 6 cut(s) 205, 270, 392, 493, 501, 773
DdeI CTNAG 5 cut(s) 234, 273, 297, 303, 543
DpnI GATC 5 cut(s) 165, 508, 594, 783, 891
DpnII GATC 5 cut(s) 163, 506, 592, 781, 889
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 2 cut(s) 87, 257
EarI CTCTTC 2 cut(s) 87, 257
Eco105I TACGTA 1 cut(s) 556
Eco147I AGGCCT 2 cut(s) 287, 472
Eco24I GRGCYC 1 cut(s) 940
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 3 cut(s) 846, 926, 1055
Eco57I CTGAAG 2 cut(s) 317, 899
EcoRII CCWGG 2 cut(s) 472, 570
EcoRV GATATC 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 940
FaeI CATG 4 cut(s) 114, 856, 867, 1005
FatI CATG 4 cut(s) 110, 852, 863, 1001
FblI GTMKAC 2 cut(s) 17, 752
Fnu4HI GCNGC 5 cut(s) 423, 444, 981, 988, 991
FokI GGATG 4 cut(s) 401, 549, 651, 882
FriOI GRGCYC 1 cut(s) 940
Fsp4HI GCNGC 5 cut(s) 423, 444, 981, 988, 991
FspBI CTAG 1 cut(s) 1115
GlaI GCGC 1 cut(s) 6
GluI GCNGC 5 cut(s) 423, 444, 981, 988, 991
GsuI CTGGAG 1 cut(s) 88
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 182, 287, 472, 477, 938
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 114, 856, 867, 1005
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HinfI GANTC 2 cut(s) 241, 860
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 123
Hpy166II GTNNAC 5 cut(s) 18, 36, 43, 753, 763
Hpy188I TCNGA 4 cut(s) 246, 544, 610, 879
Hpy188III TCNNGA 4 cut(s) 105, 412, 647, 1115
Hpy8I GTNNAC 5 cut(s) 18, 36, 43, 753, 763
Hpy99I CGWCG 2 cut(s) 224, 227
HpyAV CCTTC 2 cut(s) 138, 241
HpyCH4III ACNGT 3 cut(s) 40, 192, 497
HpyCH4IV ACGT 5 cut(s) 45, 222, 555, 619, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 293, 328, 337
HpyF3I CTNAG 5 cut(s) 234, 273, 297, 303, 543
HpySE526I ACGT 5 cut(s) 45, 222, 555, 619, 694
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 114, 856, 867, 1005
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 5 cut(s) 163, 506, 592, 781, 889
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 2 cut(s) 199, 1036
Lsp1109I GCAGC 5 cut(s) 430, 434, 992, 999, 1002
LweI GCATC 1 cut(s) 470
MaeI CTAG 1 cut(s) 1115
MaeII ACGT 5 cut(s) 45, 222, 555, 619, 694
MaeIII GTNAC 3 cut(s) 46, 739, 1127
MalI GATC 5 cut(s) 165, 508, 594, 783, 891
MbiI CCGCTC 1 cut(s) 238
MboI GATC 5 cut(s) 163, 506, 592, 781, 889
MflI RGATCY 2 cut(s) 506, 592
MhlI GDGCHC 2 cut(s) 940, 1041
MluCI AATT 5 cut(s) 94, 516, 566, 1044, 1071
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 3 cut(s) 493, 787, 1009
MseI TTAA 4 cut(s) 467, 1023, 1047, 1062
MslI CAYNNNNRTG 1 cut(s) 868
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 474, 572
MvaI CCWGG 2 cut(s) 474, 572
MwoI GCNNNNNNNGC 4 cut(s) 120, 293, 328, 337
NdeII GATC 5 cut(s) 163, 506, 592, 781, 889
NlaIII CATG 4 cut(s) 114, 856, 867, 1005
NlaIV GGNNCC 3 cut(s) 594, 928, 938
NmeAIII GCCGAG 3 cut(s) 42, 557, 776
NmuCI GTSAC 1 cut(s) 1127
NspI RCATGY 1 cut(s) 856
PaqCI CACCTGC 1 cut(s) 437
PceI AGGCCT 2 cut(s) 287, 472
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PfeI GAWTC 1 cut(s) 860
PkrI GCNGC 5 cut(s) 424, 445, 982, 989, 992
Ple19I CGATCG 2 cut(s) 166, 892
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 556
Psp6I CCWGG 2 cut(s) 472, 570
PspGI CCWGG 2 cut(s) 472, 570
PspN4I GGNNCC 3 cut(s) 594, 928, 938
PspOMI GGGCCC 1 cut(s) 936
PspPI GGNCC 5 cut(s) 846, 926, 936, 937, 1055
PstI CTGCAG 2 cut(s) 441, 448
PsuI RGATCY 2 cut(s) 506, 592
PvuI CGATCG 2 cut(s) 166, 892
RsaI GTAC 6 cut(s) 206, 271, 393, 494, 502, 774
RsaNI GTAC 6 cut(s) 205, 270, 392, 493, 501, 773
RseI CAYNNNNRTG 1 cut(s) 868
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 4 cut(s) 467, 1023, 1047, 1062
SatI GCNGC 5 cut(s) 423, 444, 981, 988, 991
Sau3AI GATC 5 cut(s) 163, 506, 592, 781, 889
Sau96I GGNCC 5 cut(s) 846, 926, 936, 937, 1055
SbfI CCTGCAGG 1 cut(s) 441
ScaI AGTACT 2 cut(s) 271, 774
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 474, 572
SdaI CCTGCAGG 1 cut(s) 441
SduI GDGCHC 2 cut(s) 940, 1041
SfaNI GCATC 1 cut(s) 470
SfcI CTRYAG 4 cut(s) 51, 278, 437, 444
SinI GGWCC 3 cut(s) 846, 926, 1055
SmiMI CAYNNNNRTG 1 cut(s) 868
SnaBI TACGTA 1 cut(s) 556
Sse8387I CCTGCAGG 1 cut(s) 441
Sse9I AATT 5 cut(s) 94, 516, 566, 1044, 1071
SseBI AGGCCT 2 cut(s) 287, 472
SsiI CCGC 2 cut(s) 238, 895
SspMI CTAG 1 cut(s) 1115
StuI AGGCCT 2 cut(s) 287, 472
StyD4I CCNGG 2 cut(s) 472, 570
TaaI ACNGT 3 cut(s) 40, 192, 497
TaiI ACGT 5 cut(s) 48, 225, 558, 622, 697
TaqI TCGA 3 cut(s) 166, 213, 355
TaqII GACCGA 1 cut(s) 863
TasI AATT 5 cut(s) 94, 516, 566, 1044, 1071
TatI WGTACW 3 cut(s) 269, 492, 772
TfiI GAWTC 1 cut(s) 860
Tru1I TTAA 4 cut(s) 467, 1023, 1047, 1062
Tru9I TTAA 4 cut(s) 467, 1023, 1047, 1062
TscAI CASTG 3 cut(s) 43, 502, 1057
TseFI GTSAC 1 cut(s) 1127
TseI GCWGC 5 cut(s) 422, 443, 980, 987, 990
Tsp45I GTSAC 1 cut(s) 1127
TspDTI ATGAA 4 cut(s) 88, 377, 676, 852
TspGWI ACGGA 3 cut(s) 21, 585, 738
TspRI CASTG 3 cut(s) 43, 502, 1057
VpaK11BI GGWCC 3 cut(s) 846, 926, 1055
XapI RAATTY 1 cut(s) 566
XbaI TCTAGA 1 cut(s) 1114
XceI RCATGY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 630
XmiI GTMKAC 2 cut(s) 17, 752
XspI CTAG 1 cut(s) 1115
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 2 cut(s) 271, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.