AT3G43300

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007281
Physical Location & Seq
Reverse (-)
15233845 .. 15245281
11437 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G43300.1

Sequence Viewer

Length: 5277 bp
ATGGCGGCTGGTGGATTTTTGACTCGAGCATTTGATACGATGCTTAAGGAGTCTGGAGGAAAGAAGTTTCCTGATCTCCAGAAAGCTATTCAAGCTTATCAAGATGGTTCAAAGGTTGTTACGCAGGCTGCACCCTCGAGCATAGTGGAGAGTTCACAAGCTGAAGGTGGAGGTGAAAAAACTGGGGTAGAAGCAGATGAACCGCAAAAAGTCACGAGTGCTGAAGTAGCGCAGCAGGCTAGCCAGTCAAAAAGTGAGACTATAAACGTTTCCTTAGCAAATGCTGGACACACATTAGGGGGAGCGGAAGTGGAGCTTGTGCTGAAACCTCTACGCCTTGCATTTGAGACAAAGAACTTAAAAATATTTGATGCTGCTTTGGATTGTCTTCATAAACTCATTGCCTATGATCATTTGGAAGGGGATCCGGGGTTGGATGGTGGAAAAAATTCTGCACCTTTCACCGACATTCTGAACATGGTTTGCAGCTGTGTTGATAATTCATCACCAGACAGCACTGTACTCCAAGTACTGAAGGTTCTTCTTACAGCTGTTGCTTCAGGAAAGTTCAAAGTGCATGGGGAGCCATTGCTGGGAGTTATTAGAGTTTGCTATAACATTGCTCTAAACAGCAAGAGCCCAATTAACCAAGCAACTTCTAAAGCAATGCTGACTCAGATGATAAGCATTGTATTCAGGAGAATGGAGACTGACATTGTTTCCGCATCATCCACAGTGTCTCAAGAAGAACATGTTTCAGGTGACACTTCAAGCCCTAAAAATGAAGAAATAACTGCAGCTGACGAAAATGAGAAAGAAATGACCTTAGGAGATGCACTCACTCAGGCTAAAGACACAACTCTTGCATCTGTTGAAGAGCTGCATACCCTTGTGGGCGGTGCTGATATTAAGGGTTTAGAAGCCGCCCTTGACAAAGCTGTGCATCTTGAAGATGGCAAGAAGATAAAACGGGGCATCGAGCTGGAGAGCATGAGTATTGGACAGCGTGATGCATTGCTTGTTTTCCGTACCCTTTGCAAGATGGGTATGAAAGAAGATAGTGATGAAGTCACAACCAAGACCCGTATATTGTCTCTTGAGCTTCTTCAGGGTATGTTAGAAGGAGTTAGTCACTCATTTACAAAGAACTTTCACTTTATAGATTCAGTGAAAGCCTACCTCTCATATGCATTGTTGCGGGCGTCGGTTTCTCAGTCTTCTGTCATATTTCAGTATGCATCTGGTATCTTCTCCGTGCTTTTGCTGCGGTTCAGAGACAGTTTAAAAGTAAGCATGGATTGTTACCTTTCACCATATTTTTCTGATCCTAAATCTCACTCTCAGGGTGAAATTGGTATATTTTTCCCCATCATCGTCTTAAGATCATTAGATAACTCCGAGTGTCCCAATGACCAAAAGATGGGTGTTCTTAGGATGCTTGAGAAAGTCTGCAAAGATCCTCAGATGCTTGTTGATGTGTATGTAAACTATGATTGTGATCTAGAGGCCCCAAACTTGTTTGAGCGCATGGTAACAACTTTGTCTAAAATTGCTCAAGGGTCTCAGAGTGCTGATCCAAATCCTGCCATGGCTTCGCAGACAGCTTCGGTTAAAGGTTCATCCCTTCAGTGCCTGGTCAACGTTCTTAAATCACTAGTTGATTGGGAGAAAATAAGGAGAGAGGCAGAAAATAGTACAAGAAATGCAAACGAGGACTCTGCTTCTACTGGAGAGCCAATTGAAACCAAAAGCAGGGAAGATGTCCCAAGCAACTTTGAGAAGGCTAAAGCTCATAAATCCACAATGGAGGCTGCCATCTCCGAGTTCAACAGGAATTCAGTGAAGGGTGTCGAATATCTAATTGCAAACAAGTTGGTTGAAAGGAATCCTGCTTCAGTTGCACAGTTTCTGAGAAGTACTTCGAGTCTGAGCAAGGTTATGATTGGCGATTACCTGGGCCAACACGAGGAGTTTCCTCTTGCTGTCATGCATGCATATGTTGATTCAATGAAATTTTCAGAAATGAAGTTTCATTCGGCTATTCGTGAATTTCTCAAAGGTTTTAGACTTCCTGGAGAGGCCCAAAAAATTGATCGTATTATGGAAAAGTTCGCAGAAAGATATTGCGCAGACAATCCGGGTCTTTTCAAGAATGCAGATACAGCCTACGTTCTAGCCTATGCAGTTATCATGTTAAATACAGATGCGCATAATCCTATGGTTTGGCCTAAGATGTCAAAATCAGATTTCACACGTATGAATGCCACTAATGATCCTGAAGATTGTGCTCCAACTGAACTTCTGGAAGAGATCTATGATTCTATTGTACAAGAAGAAATTAAACTAAAAGACGATGACACCATGAAGAAGCTCAGTAGTCAAAGGCCAGGAGGAGAAGAAAGAGGTGGTCTTGTCAGCATTCTTAATCTGGGTTTGCCAAAGAGAATATCAGCAGCTGATGCTAAATCTGAGACTGAGGACATTGTTAGGAAAACACAGGAAATTTTCCGAAAGCATGGAGTGAAAAGAGGAGTCTTTCACACGGTTGAGCAAGTGGACATTATAAGGCCCATGGTGGAAGCTGTTGGGTGGCCTCTGCTTGCTGCTTTCTCCGTTACAATGGAAGTAGGTGATAACAAACCAAGGATTCTTCTCTGCATGGAGGGATTTAAAGCTGGAATACATATTGCTTATGTTCTTGGAATGGATACAATGCGATATGCATTTCTAACATCGCTTGTCAGGTTCACTTTCTTGCATGCTCCAAAAGAAATGCGGAGCAAAAATGTTGAAGCATTGAGGATATTACTGGGGTTGTGTGACTCAGAACCTGACACCCTTCAAGATACTTGGAATGCAGTTTTAGAATGTGTTTCTAGGCTGGAATTCATTATTTCTACTCCCGGAATTGCTGCAACAGTAATGCATGGATCAAACCAGATCTCCAGGGATGGGGTTGTTCAATCATTGAAGGAGTTAGCCGGGAGACCTGCTGAACAAGTTTTTGTAAACAGTGTCAAGCTGCCCAGTGAATCTGTTGTGGAGTTTTTTACTGCGCTATGTGGTGTTTCAGCTGAAGAATTGAAGCAGTCTCCTGCCCGTGTTTTCAGCTTGCAGAAGCTAGTTGAGATCAGTTATTACAATATAGCACGTATCCGAATGGTCTGGGCAAGAATATGGTCTGTCCTTGCCGAACATTTCGTATCTGCTGGTAGCCATCATGATGAAAAGATTGCAATGTATGCCATAGATTCTCTGAGACAGCTCGGGATGAAGTATTTAGAACGTGCTGAGCTCACCAATTTCACTTTCCAAAATGATATTCTCAAACCGTTCGTTATTATCATGCGGAATACTCAAAGTCAGACCATAAGGAGCCTAATTGTTGACTGCATCGTTCAGATGATAAAATCTAAAGTTGGAAGTATCAAATCGGGATGGAGGAGTGTTTTTATGATATTTACAGCAGCTGCAGATGACGAAGTTGAATCGATAGTTGAAAAATCATTTGAGAATGTTGAGCAAGTTATTCTGGAACACTTTGACCAGGTGATAGGTGACTGCTTCATGGATTGTGTCAATTGTCTCATCAGGTTTGCCAATAACAAAGCTTCAGACCGGATAAGCCTGAAAGCTATTGCCCTTCTCAGAATATGTGAGGATCGGCTTGCAGAGGGACTTATACCCGGTGGTGTTCTTAAGCCCGTTGATGGCAATGAGGATGAAACTTTTGATGTGACAGAGCATTACTGGTTTCCGATGCTTGCCGGTCTATCTGATCTCACATCTGATTATAGGCCCGAAGTTAGAAACTGTGCTCTGGAGGTGTTGTTTGATTTGCTAAATGAAAGGGGAAACAAGTTCTCCACACCTTTCTGGGAGAGTATCTTCCATCGCATCTTGTTTCCAATTTTTGATCATGTGAGTCATGCTGGAAAGGAAAGCTTAATATCTTCCGGGGATGTAAAATTTCGTGAAACAAGCATTCATTCCCTTCAGCTCCTCTGCAATCTCTTCAATACGTTCTACAAGGAAGTATGTTTTATGCTGCCTCCACTTTTAAGTTTGCTCCTAGACTGTGCGAAGAAATCAGATCAGACAGTTGTTTCAATTTCATTAGGAGCATTGGTTCACCTCATCGAGGTTGGAGGCCACCAATTTAGTGAGGGAGACTGGGATATGCTCTTGAAAAGCATAAGAGATGCATCATACACAACTCAACCGCTGGAGCTGTTGAATGCTTTGAGTTTTGACAATCCGAAAAAGAACCTAGTTTTGGCAGGAGACATAGAGGCCGATGCCTCTGATTCTCCACGAGTTGATCGTAATCCGGACGATATTAAAGATAATGGGAAAGTGTCCGCCCAGGCATCTCCAAGGATTGGTACTCATGGTACTTCCCTAGAATCTGGGATACCGCCTAAGGCTGATGGTTCGGAAGGTCGTCCATCGTCATCTGGAAGGGCTCAAAAGGATGTGGATGATGTGAATCTGCAGCGGAGTCAGACTTTTGGCCAAAGATTCATGGACAATCTCTTCCTCCGGAATCTCACATCTCAACCAAAAAGCTCTGTTGCAGAAGTGACTGTACCCTCCTCTCCATATAAGCATGAAGATCCTACAGAGCCTGACAGCAGAGAAGAAGAGAGTCCAGCATTGGGAGCTATTAGAGGAAAATGCATCACACAATTACTACTACTTGGTGCTATCAACAGCATCCAGCAAAAATACTGGAGTAATTTGAAAACCCCACAGAAGATTGCGATTATGGACATCTTATTCTCTTTCATCGAATTTGCTTCTTCCTACAATTCATATTCTAACCTTAGAACACGTATGAATCACATTCCCACAGAGAGGCCACCTCTAAACCTTCTCCGGCAAGAGCTGGAAGGAACCACCATATATTTGGACGTCTTGCAAAAGACAACTTCTGGGCTTGCGGATGATGCATCTAACTCGGAAGATAGACTAGAAGGTGCAGCAGAAGAAAAATTGGTATCGTTCTGTGAACAGGTTCTGAAAGAAACATCTGATCTCCAGTCCACTTTGGGGGAGACTACTAACATGGATGTTCATCGGGTACTGGAGCTACGTTCTCCCGTGATTGTGAAGGTTTTGGAAGGCATGTGCTTCATGAACAACACAATATTCAGGAAGCACATGAGAGAGTTCTACCCTCTGCTCACGAGGCTCGTTTGCTGTGAACAGATGGAGATAAGAGGTGCACTAGCCAACCTTTTCAAAGCACAATTGAAGCCACTTTTGCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1758

Amino Acids

195.05

Weight (kDa)

5.39

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 81 - 238 1.1e-26 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 335 - 438 4.3e-18 Mon2/Sec7/BIG1-like, HUS domain
Sec7-like_HUS PF12783 444 - 506 1.1e-14 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 599 - 780 8.7e-70 Sec7 domain
Sec7-like_HDS PF09324 1092 - 1174 5.3e-28 Mon2/Sec7/BIG1-like, HDS
BIG2_C PF20252 1562 - 1749 7.7e-29 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2564
AatII GACGTC 1 cut(s) 4917
Acc16I TGCGCA 2 cut(s) 2128, 2208
Acc36I ACCTGC 1 cut(s) 2998
AccB7I CCANNNNNTGG 2 cut(s) 1420, 4382
AccBSI CCGCTC 1 cut(s) 305
AccIII TCCGGA 2 cut(s) 4330, 4542
AclI AACGTT 2 cut(s) 267, 1641
AclWI GGATC 9 cut(s) 419, 432, 1319, 1451, 1568, 2267, 2938, 3669, 4610
AcoI YGGCCR 1 cut(s) 4513
AcsI RAATTY 8 cut(s) 448, 1834, 2012, 2048, 2502, 2885, 3968, 4793
AcyI GRCGYC 2 cut(s) 1202, 4914
AflII CTTAAG 3 cut(s) 44, 1378, 3698
AflIII ACRYGT 3 cut(s) 751, 2252, 4832
AhlI ACTAGT 1 cut(s) 1654
AjnI CCWGG 7 cut(s) 1632, 1953, 2071, 2386, 2945, 3546, 4365
AjuI GAANNNNNNNTTGG 6 cut(s) 3293, 3306, 3325, 3338, 4259, 4291
AloI GAACNNNNNNTCC 2 cut(s) 4113, 4145
Alw21I GWGCWC 4 cut(s) 2290, 3297, 3820, 5230
Alw44I GTGCAC 1 cut(s) 5226
AlwI GGATC 9 cut(s) 419, 432, 1319, 1451, 1568, 2267, 2938, 3669, 4610
AlwNI CAGNNNCTG 7 cut(s) 1633, 1909, 2456, 2831, 3470, 4628, 5020
Ama87I CYCGRG 3 cut(s) 24, 136, 3266
Aor13HI TCCGGA 2 cut(s) 4330, 4542
ApaLI GTGCAC 1 cut(s) 5226
ApoI RAATTY 8 cut(s) 448, 1834, 2012, 2048, 2502, 2885, 3968, 4793
Asp700I GAANNNNTTC 3 cut(s) 448, 1918, 5016
AspLEI GCGC 5 cut(s) 232, 1527, 2129, 2209, 3058
AspS9I GGNCC 5 cut(s) 1507, 1957, 2080, 2568, 3798
AsuC2I CCSGG 6 cut(s) 429, 2139, 2904, 2983, 3687, 3958
AsuNHI GCTAGC 1 cut(s) 239
AvaI CYCGRG 3 cut(s) 24, 136, 3266
AxyI CCTNAGG 2 cut(s) 826, 4422
BaeGI GKGCMC 1 cut(s) 5230
BalI TGGCCA 1 cut(s) 4515
BamHI GGATCC 1 cut(s) 424
BanII GRGCYC 3 cut(s) 641, 3297, 4468
BarI GAAGNNNNNNTAC 2 cut(s) 4572, 4604
BauI CACGAG 4 cut(s) 214, 1964, 4314, 5188
BbsI GAAGAC 2 cut(s) 380, 1209
Bbv12I GWGCWC 4 cut(s) 2290, 3297, 3820, 5230
BcgI CGANNNNNNTGC 4 cut(s) 1700, 1734, 4941, 4975
BciT130I CCWGG 7 cut(s) 1634, 1955, 2073, 2388, 2947, 3548, 4367
BciVI GTATCC 3 cut(s) 2701, 3164, 4407
BclI TGATCA 2 cut(s) 409, 3916
BcnI CCSGG 6 cut(s) 429, 2139, 2904, 2983, 3687, 3958
BcuI ACTAGT 1 cut(s) 1654
BfmI CTRYAG 4 cut(s) 795, 3471, 4493, 4620
BfrI CTTAAG 3 cut(s) 44, 1378, 3698
BfuAI ACCTGC 1 cut(s) 2998
BfuI GTATCC 3 cut(s) 2701, 3164, 4407
BglII AGATCT 2 cut(s) 2310, 2940
BlpI GCTNAGC 1 cut(s) 3291
BmcAI AGTACT 2 cut(s) 531, 1918
BmeT110I CYCGRG 3 cut(s) 24, 136, 3266
BmgT120I GGNCC 5 cut(s) 1507, 1957, 2080, 2568, 3798
BmiI GGNNCC 5 cut(s) 426, 585, 1509, 3377, 4897
BmrI ACTGGG 4 cut(s) 192, 2819, 3021, 4183
BmtI GCTAGC 1 cut(s) 243
BmuI ACTGGG 4 cut(s) 192, 2819, 3021, 4183
BpiI GAAGAC 2 cut(s) 380, 1209
BplI GAGNNNNNCTC 4 cut(s) 822, 854, 4849, 4881
Bpu10I CCTNAGC 1 cut(s) 274
Bpu1102I GCTNAGC 1 cut(s) 3291
BpuEI CTTGAG 4 cut(s) 726, 1118, 1460, 1539
BpuMI CCSGG 6 cut(s) 429, 2139, 2904, 2983, 3687, 3958
Bsa29I ATCGAT 1 cut(s) 3491
BsaAI YACGTR 3 cut(s) 2255, 3152, 4835
BsaBI GATNNNNATC 5 cut(s) 1497, 1578, 4326, 4488, 5076
BsaHI GRCGYC 2 cut(s) 1202, 4914
BsaI GGTCTC 2 cut(s) 1566, 2980
BsaJI CCNNGG 9 cut(s) 428, 1587, 1954, 2571, 2642, 2946, 3957, 4365, 4376
BsaWI WCCGGW 3 cut(s) 3618, 4330, 4542
Bse118I RCCGGY 1 cut(s) 3767
Bse21I CCTNAGG 2 cut(s) 826, 4422
Bse3DI GCAATG 7 cut(s) 399, 587, 618, 672, 1013, 3243, 3721
Bse8I GATNNNNATC 5 cut(s) 1497, 1578, 4326, 4488, 5076
BseAI TCCGGA 2 cut(s) 4330, 4542
BseBI CCWGG 7 cut(s) 1634, 1955, 2073, 2388, 2947, 3548, 4367
BseCI ATCGAT 1 cut(s) 3491
BseDI CCNNGG 9 cut(s) 428, 1587, 1954, 2571, 2642, 2946, 3957, 4365, 4376
BseJI GATNNNNATC 5 cut(s) 1497, 1578, 4326, 4488, 5076
BseMI GCAATG 7 cut(s) 399, 587, 618, 672, 1013, 3243, 3721
BseRI GAGGAG 6 cut(s) 1982, 2406, 2544, 3457, 3992, 4585
BseSI GKGCMC 1 cut(s) 5230
BseYI CCCAGC 1 cut(s) 592
BsgI GTGCAG 3 cut(s) 114, 438, 5001
BshVI ATCGAT 1 cut(s) 3491
BsiHKAI GWGCWC 4 cut(s) 2290, 3297, 3820, 5230
BsiHKCI CYCGRG 3 cut(s) 24, 136, 3266
BslFI GGGAC 3 cut(s) 1389, 1748, 3690
BsmFI GGGAC 3 cut(s) 1389, 1748, 3690
BsmI GAATGC 6 cut(s) 2158, 2266, 2418, 2860, 3984, 4243
Bso31I GGTCTC 2 cut(s) 1566, 2980
BsoBI CYCGRG 3 cut(s) 24, 136, 3266
Bsp1286I GDGCHC 6 cut(s) 641, 2290, 3297, 3820, 4468, 5230
Bsp13I TCCGGA 2 cut(s) 4330, 4542
Bsp1407I TGTACA 1 cut(s) 2326
Bsp1720I GCTNAGC 1 cut(s) 3291
Bsp19I CCATGG 2 cut(s) 1587, 2571
BspDI ATCGAT 1 cut(s) 3491
BspEI TCCGGA 2 cut(s) 4330, 4542
BspHI TCATGA 2 cut(s) 3220, 5136
BspLI GGNNCC 5 cut(s) 426, 585, 1509, 3377, 4897
BspMAI CTGCAG 3 cut(s) 799, 3475, 4497
BspMI ACCTGC 1 cut(s) 2998
BspOI GCTAGC 1 cut(s) 243
BspPI GGATC 9 cut(s) 419, 432, 1319, 1451, 1568, 2267, 2938, 3669, 4610
BspQI GCTCTTC 1 cut(s) 870
BspTI CTTAAG 3 cut(s) 44, 1378, 3698
BspTNI GGTCTC 2 cut(s) 1566, 2980
BsrBI CCGCTC 1 cut(s) 305
BsrDI GCAATG 7 cut(s) 399, 587, 618, 672, 1013, 3243, 3721
BsrFI RCCGGY 1 cut(s) 3767
BsrGI TGTACA 1 cut(s) 2326
BssAI RCCGGY 1 cut(s) 3767
BssECI CCNNGG 9 cut(s) 428, 1587, 1954, 2571, 2642, 2946, 3957, 4365, 4376
BssNI GRCGYC 2 cut(s) 1202, 4914
BssSI CACGAG 4 cut(s) 214, 1964, 4314, 5188
BssT1I CCWWGG 4 cut(s) 1587, 2571, 2642, 4376
Bst2BI CACGAG 4 cut(s) 214, 1964, 4314, 5188
Bst2UI CCWGG 7 cut(s) 1634, 1955, 2073, 2388, 2947, 3548, 4367
Bst6I CTCTTC 5 cut(s) 870, 2301, 4019, 4541, 4638
BstACI GRCGYC 2 cut(s) 1202, 4914
BstAFI CTTAAG 3 cut(s) 44, 1378, 3698
BstAPI GCANNNNNTGC 2 cut(s) 2459, 3242
BstAUI TGTACA 1 cut(s) 2326
BstBAI YACGTR 3 cut(s) 2255, 3152, 4835
BstDSI CCRYGG 2 cut(s) 1587, 2571
BstENI CCTNNNNNAGG 1 cut(s) 4139
BstHHI GCGC 5 cut(s) 232, 1527, 2129, 2209, 3058
BstNI CCWGG 7 cut(s) 1634, 1955, 2073, 2388, 2947, 3548, 4367
BstNSI RCATGY 4 cut(s) 755, 1994, 2762, 5131
BstSFI CTRYAG 4 cut(s) 795, 3471, 4493, 4620
BstSLI GKGCMC 1 cut(s) 5230
BstV2I GAAGAC 2 cut(s) 380, 1209
BstX2I RGATCY 5 cut(s) 424, 1456, 2310, 2940, 4615
BstXI CCANNNNNNTGG 1 cut(s) 4909
BstYI RGATCY 5 cut(s) 424, 1456, 2310, 2940, 4615
Bsu15I ATCGAT 1 cut(s) 3491
Bsu36I CCTNAGG 2 cut(s) 826, 4422
BsuI GTATCC 3 cut(s) 2701, 3164, 4407
BsuTUI ATCGAT 1 cut(s) 3491
BtgI CCRYGG 2 cut(s) 1587, 2571
BtgZI GCGATG 2 cut(s) 2718, 3878
BtsIMutI CAGTG 7 cut(s) 516, 741, 1173, 1634, 1845, 3019, 3034
BveI ACCTGC 1 cut(s) 2998
CaiI CAGNNNCTG 7 cut(s) 1633, 1909, 2456, 2831, 3470, 4628, 5020
CciI TCATGA 2 cut(s) 3220, 5136
CfoI GCGC 5 cut(s) 232, 1527, 2129, 2209, 3058
Cfr10I RCCGGY 1 cut(s) 3767
Cfr13I GGNCC 5 cut(s) 1507, 1957, 2080, 2568, 3798
ClaI ATCGAT 1 cut(s) 3491
CseI GACGC 1 cut(s) 1191
CsiI ACCWGGT 1 cut(s) 3546
DraI TTTAAA 2 cut(s) 1284, 2671
EaeI YGGCCR 1 cut(s) 4513
Eam1104I CTCTTC 5 cut(s) 870, 2301, 4019, 4541, 4638
EarI CTCTTC 5 cut(s) 870, 2301, 4019, 4541, 4638
EciI GGCGGA 1 cut(s) 4351
Ecl136II GAGCTC 1 cut(s) 3295
Eco130I CCWWGG 4 cut(s) 1587, 2571, 2642, 4376
Eco24I GRGCYC 3 cut(s) 641, 3297, 4468
Eco31I GGTCTC 2 cut(s) 1566, 2980
Eco53kI GAGCTC 1 cut(s) 3295
Eco81I CCTNAGG 2 cut(s) 826, 4422
Eco88I CYCGRG 3 cut(s) 24, 136, 3266
EcoICRI GAGCTC 1 cut(s) 3295
EcoNI CCTNNNNNAGG 1 cut(s) 4139
EcoO109I RGGNCCY 1 cut(s) 1507
EcoRI GAATTC 2 cut(s) 1834, 2885
EcoRII CCWGG 7 cut(s) 1632, 1953, 2071, 2386, 2945, 3546, 4365
EcoT14I CCWWGG 4 cut(s) 1587, 2571, 2642, 4376
EcoT38I GRGCYC 3 cut(s) 641, 3297, 4468
ErhI CCWWGG 4 cut(s) 1587, 2571, 2642, 4376
FalI AAGNNNNNCTT 6 cut(s) 300, 332, 912, 944, 3929, 3961
FaqI GGGAC 3 cut(s) 1389, 1748, 3690
FauI CCCGC 1 cut(s) 1191
FauNDI CATATG 2 cut(s) 1186, 1996
FbaI TGATCA 2 cut(s) 409, 3916
FriOI GRGCYC 3 cut(s) 641, 3297, 4468
FspAI RTGCGCAY 1 cut(s) 2208
FspI TGCGCA 2 cut(s) 2128, 2208
GlaI GCGC 5 cut(s) 231, 1526, 2128, 2208, 3057
GsaI CCCAGC 1 cut(s) 596
HgaI GACGC 1 cut(s) 1191
HhaI GCGC 5 cut(s) 232, 1527, 2129, 2209, 3058
Hin1I GRCGYC 2 cut(s) 1202, 4914
Hin6I GCGC 5 cut(s) 230, 1525, 2127, 2207, 3056
HinP1I GCGC 5 cut(s) 230, 1525, 2127, 2207, 3056
HincII GTYRAC 2 cut(s) 1639, 3388
HindII GTYRAC 2 cut(s) 1639, 3388
HindIII AAGCTT 3 cut(s) 93, 3609, 3943
Hpy99I CGWCG 1 cut(s) 1207
Hsp92I GRCGYC 2 cut(s) 1202, 4914
HspAI GCGC 5 cut(s) 230, 1525, 2127, 2207, 3056
Kpn2I TCCGGA 2 cut(s) 4330, 4542
Ksp22I TGATCA 2 cut(s) 409, 3916
LguI GCTCTTC 1 cut(s) 870
MabI ACCWGGT 1 cut(s) 3546
MbiI CCGCTC 1 cut(s) 305
MfeI CAATTG 3 cut(s) 1737, 3580, 5252
MflI RGATCY 5 cut(s) 424, 1456, 2310, 2940, 4615
MhlI GDGCHC 6 cut(s) 641, 2290, 3297, 3820, 4468, 5230
MlsI TGGCCA 1 cut(s) 4515
MluNI TGGCCA 1 cut(s) 4515
MmeI TCCRAC 4 cut(s) 414, 2315, 3400, 4126
Mox20I TGGCCA 1 cut(s) 4515
MroI TCCGGA 2 cut(s) 4330, 4542
MroXI GAANNNNTTC 3 cut(s) 448, 1918, 5016
MscI TGGCCA 1 cut(s) 4515
MslI CAYNNNNRTG 3 cut(s) 1995, 2255, 3222
Msp20I TGGCCA 1 cut(s) 4515
MspA1I CMGCKG 8 cut(s) 489, 551, 800, 2456, 3074, 3470, 4225, 4498
MspCI CTTAAG 3 cut(s) 44, 1378, 3698
MunI CAATTG 3 cut(s) 1737, 3580, 5252
Mva1269I GAATGC 6 cut(s) 2158, 2266, 2418, 2860, 3984, 4243
MvaI CCWGG 7 cut(s) 1634, 1955, 2073, 2388, 2947, 3548, 4367
NciI CCSGG 6 cut(s) 429, 2139, 2904, 2983, 3687, 3958
NcoI CCATGG 2 cut(s) 1587, 2571
NdeI CATATG 2 cut(s) 1186, 1996
NheI GCTAGC 1 cut(s) 239
NlaIV GGNNCC 5 cut(s) 426, 585, 1509, 3377, 4897
NmuCI GTSAC 8 cut(s) 211, 761, 1069, 1130, 2819, 3557, 3736, 4582
NsbI TGCGCA 2 cut(s) 2128, 2208
NspI RCATGY 4 cut(s) 755, 1994, 2762, 5131
PaeI GCATGC 2 cut(s) 1994, 2762
PaeR7I CTCGAG 2 cut(s) 24, 136
PagI TCATGA 2 cut(s) 3220, 5136
PciI ACATGT 1 cut(s) 751
PciSI GCTCTTC 1 cut(s) 870
PcsI WCGNNNNNNNCGW 1 cut(s) 4321
PctI GAATGC 6 cut(s) 2158, 2266, 2418, 2860, 3984, 4243
PdmI GAANNNNTTC 3 cut(s) 448, 1918, 5016
PflMI CCANNNNNTGG 2 cut(s) 1420, 4382
PfoI TCCNGGA 2 cut(s) 2071, 2902
Ppu21I YACGTR 3 cut(s) 2255, 3152, 4835
PscI ACATGT 1 cut(s) 751
PsiI TTATAA 1 cut(s) 2564
Psp124BI GAGCTC 1 cut(s) 3297
Psp1406I AACGTT 2 cut(s) 267, 1641
Psp6I CCWGG 7 cut(s) 1632, 1953, 2071, 2386, 2945, 3546, 4365
PspFI CCCAGC 1 cut(s) 592
PspGI CCWGG 7 cut(s) 1632, 1953, 2071, 2386, 2945, 3546, 4365
PspN4I GGNNCC 5 cut(s) 426, 585, 1509, 3377, 4897
PspPI GGNCC 5 cut(s) 1507, 1957, 2080, 2568, 3798
PspXI VCTCGAGB 2 cut(s) 24, 136
PsrI GAACNNNNNNTAC 2 cut(s) 522, 554
PstI CTGCAG 3 cut(s) 799, 3475, 4497
PstNI CAGNNNCTG 7 cut(s) 1633, 1909, 2456, 2831, 3470, 4628, 5020
PsuI RGATCY 5 cut(s) 424, 1456, 2310, 2940, 4615
PvuII CAGCTG 6 cut(s) 489, 551, 800, 2456, 3074, 3470
RseI CAYNNNNRTG 3 cut(s) 1995, 2255, 3222
SacI GAGCTC 1 cut(s) 3297
SapI GCTCTTC 1 cut(s) 870
Sau96I GGNCC 5 cut(s) 1507, 1957, 2080, 2568, 3798
ScaI AGTACT 2 cut(s) 531, 1918
SduI GDGCHC 6 cut(s) 641, 2290, 3297, 3820, 4468, 5230
SexAI ACCWGGT 1 cut(s) 3546
SfcI CTRYAG 4 cut(s) 795, 3471, 4493, 4620
Sfr274I CTCGAG 2 cut(s) 24, 136
SlaI CTCGAG 2 cut(s) 24, 136
SmiMI CAYNNNNRTG 3 cut(s) 1995, 2255, 3222
SmlI CTYRAG 9 cut(s) 24, 44, 136, 741, 1097, 1378, 1439, 1554, 3698
SmoI CTYRAG 9 cut(s) 24, 44, 136, 741, 1097, 1378, 1439, 1554, 3698
SpeI ACTAGT 1 cut(s) 1654
SphI GCATGC 2 cut(s) 1994, 2762
SspI AATATT 2 cut(s) 366, 5151
SstI GAGCTC 1 cut(s) 3297
StyI CCWWGG 4 cut(s) 1587, 2571, 2642, 4376
TaqI TCGA 8 cut(s) 25, 137, 978, 1851, 1922, 3491, 4140, 4791
TatI WGTACW 5 cut(s) 520, 529, 1694, 1916, 2326
TauI GCSGC 2 cut(s) 8, 926
TscAI CASTG 7 cut(s) 523, 741, 1173, 1634, 1845, 3019, 3034
TseFI GTSAC 8 cut(s) 211, 761, 1069, 1130, 2819, 3557, 3736, 4582
Tsp45I GTSAC 8 cut(s) 211, 761, 1069, 1130, 2819, 3557, 3736, 4582
TspGWI ACGGA 3 cut(s) 1016, 1243, 2602
TspRI CASTG 7 cut(s) 523, 741, 1173, 1634, 1845, 3019, 3034
Van91I CCANNNNNTGG 2 cut(s) 1420, 4382
Vha464I CTTAAG 3 cut(s) 44, 1378, 3698
VneI GTGCAC 1 cut(s) 5226
XagI CCTNNNNNAGG 1 cut(s) 4139
XapI RAATTY 8 cut(s) 448, 1834, 2012, 2048, 2502, 2885, 3968, 4793
XbaI TCTAGA 1 cut(s) 1501
XceI RCATGY 4 cut(s) 755, 1994, 2762, 5131
XcmI CCANNNNNNNNNTGG 1 cut(s) 4906
XhoI CTCGAG 2 cut(s) 24, 136
XmnI GAANNNNTTC 3 cut(s) 448, 1918, 5016
ZraI GACGTC 1 cut(s) 4915
ZrmI AGTACT 2 cut(s) 531, 1918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.