RLG00000016646

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007281
Physical Location & Seq
Reverse (-)
8539105 .. 8552241
13137 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016646

Sequence Viewer

Length: 5976 bp
ATGTCTATGGGTTTAGTTGTCGATACATTCTCGTCTTCGGACCTGTTAGTGAGCGACGTCCTCTACAAGGCGGCCTTCGTAATCGCGCTGCTGAGATGGGCCTTCTACTGGGCCCTGAGACTCCGCCGGAGAATCTTGTCCGACGAAGATTCCGATCTGTTAGATACGGAGCTTGACTATGAAGTAGTACAGTCCTCTTCCTGTAACACGTCTTCTCCTTCGTCGTCGACCCAGCTGATCAGAGACAGTCTGATACGGACGACGTTCGAGGAGGTGACGAGACGACAGGGAAACAGCTCTCGTTGGGACACCTGCGCCGTCTGCCTGAGCCAGTTGGAGATGGGGGACGAGGTCCGGGAGCTGAGGAACTGCTGCCACGTCTTCCACACCGAGTGCATCGACAGGTGGCTGGACTACAACGGCCATGATCATCACGATCACGGCGCCCATGACGACAACCACAAGACGTGCCCCCTCTGCAGGACGCCTCTGCTGACGTCATCGCAGATCCAGAGCCTGAGCTGGGAGCAGAGCTTCGTTTCTAGTTCCGCTGAGTCTCAACGCCCCAAACCCCGCGGAGTCTCTGGAGCCAAAGTTCCCGATCTCAACTCACCTTCTCTCAGCTCTCCGCTCACTCCACCCCGTCGCGCTCACTCACATTCTTATCGTCTTCAACGATCAGCCTCATCCAGGCGGTTCGGGTCGGAGTTGAAGATGGCGGGAGGCGCAGCCGGCGGCTTCGTGACGAGAGCCTATGAGTCCATGCTCAAAGAGTGCTCACCGAAAAAGTATTCCGATCTTCAGAAGGCAATTCAGGCTTATTTAGACAATGTGAAAGCGGTTAATCAGGCTCAACAAACCACACCACAAAGTGCTCCAAGTGAGAAGAATCAAGCTACAGATGGTGATGGAAGTTCTCTTGAAACAGAAGGTGAAGCTCCAAAAACTGGTGCAGAACCAGGTCAGTCTCAAACATCCTCAGACACTGCCGAGGAGGCAGATTCTGTTGGCAGGCCAGTGAGCATAAGTGGAACTGTTTCAACTGTCCTAGCAACTGCTGGGAACACCTTAGAAGGCGCTCAGGCGGAGCTTGTTTTGAATCCCCTGCGACTTGCATTCGATACAAAGAACTTGAAAGTTCTAGAACCTGCTCTGGATTGTTTTCATAAACTCATTGCGTACGATCATTTAGAGGGAGATCCTGGTTTAGACGATAAAAGTGTTCCGGTGTTTACCGACATTTTGAACAGGGTTTGCAGTTGTGTTGACAATAATTCACCTGATAGTACCGTTCTTCAAGTGTTGAAGGTCCTTCTTACTGCAGTGGCATCCACAAAGTTTAGAGTACATGGGGAACCTTTGCTGGGAGTTATCAGAGTGTGCTATAATATTGCTCTGCACAGCAAGAGTCCTGTCAACCAAGCTACATCAAAGGCAATGCTTACGCAGATGATCAGCATCATATTCAGGAGAATGGAGACCGATCCGGTTTCATCATCTGGTTCTGTTGGACAAAATATAAATACAGCACAAAATTCAAATACAGAAGCTGAAGAAACTTCTGTGGCAGACCAAAATGAGAAAGGAATGACTTTAGGAGATCAACTTAACCAGGTTAAAGAGACACCCGTTGCATCTGTTGAGGAACTTCATAATCTTGCAGGTGGTGCTGACATCAAGGGTTTAGAGGCCGTCCTGGACAAAGCCGTGCATCATGAAGATGGCAAAAAGATAACCAGAGGGATTGACCTGGAGAGTATGAGCATAGTTCAACGTGATGCTTTACTGGTTTTCCGAACCCTGTGCAAGATGGGCATGAAGGAAGATAACAACGAAGTTACCTTGAAGACGAGGATTTTATCTCTTGAGCTTCTGCAGGGTTTGTTGGAAGGAGTTGGTCATCCATTTACTAGGAACTTCCATTTTATTGATTCCGTGAAAGCATACCTATCATATGCTTTGTTGCGGGCTTCAGTTTCGCCGTCCCCTATCATATTTCAGTATGCAACTGGAATTTTTTTAGTGCTTTTGTTGCGGTTTAGAGAGAGTCTCAAGGGTGAAATAGGCATCTTCTTTCCCTTGATAGTTCTCCGGTCCCTGGATGGCTCGGATTTACCCATGAACCAGAAAATGAGTGTTCTTAGGATGTTAGAAAAAGTTTGCAAGGATCCTCAGATGCTTGTTGACATATTTGTGAACTATGACTGTGATCTTGAGGCACCGAACTTGTTTGAGCGGATGGTTACAACTCTATCCAGAATATCTCAAGGGACGCAAAATGCAGATCCGAATATGGCTGCTGCATCCCAGACTACTTCAATTAAGGGTTCTTCTCTTCAGTGTCTTGTGAATGTGCTTAAATCACTTGTAGATTGGGAAAAGTCACGTGGAGAATCAGATAGCCAGAGTAAAAACACTCGGTCTCTGGAAGGAGATGCTTCAGCTAAAGAGTCTATTGATGTGAAAAGCAGGCAAGATACAACCACTAACTTTGAGAAGGCAAAAGCTCATAAGTCTACACTGGAAGCTGCCATATCTGAGTTCAACAGGCGACCAGTGACGGGTGTAGAGTATCTGAGATCAAATAAATTAGTGGAAAACACACCTAGTTCAGTTGCCCAATTTTTGAAAAGTACTCCCAGTTTGGACAAGGCTATGATTGGGGAATATTTGGGCCATCATGAAGAATTTCCGCTTTCTGTTATGCATGCTTATGTAGATTCCATGAAATTTTCGGGAATGAAGTTTGATACTGCAATTCGTGAACTTCTTAAAGGATTCCGACTTCCAGGGGAAGCTCAAAAGATAGATCGCATCATGGAAAAGTTTGCAGAAAGATACTGTGCAGATAATCCAGGCCTCTTCAAAAATGCAGATACTGCTTACGTTCTTGCATATGCAGTTATTATGTTGAATACTGATGCGCATAATCCAATGGTGTGGCCTAAGATGTCCAAGTCAGATTTCGTGCGCATGAATGCCACTGAAGATCCAGAAGATTGTGCTCCAACAGAACTTCTGGAAGAAATCTATGACTCAATTATTAAAGAAGAGATAAAGATGAAAGATGAGAGTACTAGTCTCGAGAAAAGTGGCAAATATAAGCCAGAAGGTGAAGAGAGAGGACGACTTGTCAGTATTCTTAATCTGGCTCTTCCCAGAAGGACACTGTCTTCTGATACCAAGTCTGAAAGTGAAGCTATCATAAAGAGAGCACAAGATATATTTAGGAATCAAGGAGCACAAAGAGGGGTCTTCCATACTACACAGCAGATAGAGCTTGTAAGGCCTATGGTAGAAGCTGTTGGATGGCCTTTGCTTGCTACCTTCTCTGTTACAATGGAGGAGGGTGAGAATAAGTCGAGGGTTGTTCTCTGCATGGAAGGATTTAAAGCAGGTATACACATTACACATGTTCTGGGGATGGATACCATGCGCTATGCCTTCTTAACATCTCTGGTCAGATTTACATTCTTGCATGCTCCAAAGGAAATGCGTAGTAAAAATGTGGAAGCATTGCGAACTCTACTAGCTCTTTGTGACTCAGAGACTGGGTCCCTTCAAGACACATGGAATGCAGTTTTGGAATGTGTTTCACGGCTTGAATACATCACTTCAACCCCTGGTATTGCTGCAACAGTCATGCAGGGATCAAACCAAATCTCTAAAGATGCTGTTCTTCAATCTCTAAGAGAATTGGCTGGAAAACCTTCTGAGCAAGTATTTGTGAACAGTGTTCAGCTTCCCAGTGATTCTGTAGTGGAGTTCTTCACTGCACTCTGTGGTGTATCAGCGGAAGAACTAAAACAAAGTCCAGCTCGGGTTTTTAGCTTGCAGAAACTTGTTGAGATCAGCTACTACAACATGGCTCGTATACGCATGGTCTGGGCTAGGATATGGTCTGTTTTGGCAAACCACTTTATTTCAGCTGGGAGTCATCATGATGAGAAAATTGCTATGTATGCCATAGATTCTCTAAGGCAACTTGGTATGAAGTATCTGGAGCGAGCTGAACTTGCCAATTTCACATTCCAAAATGACATTCTTAAGCCTTTTGTTGTTCTTATGCGCAATAGTCGAAGTGAAACCATAAGGGGCCTAATTGTTGACTGCATTGTTCAAATGATAAAATCTAAAGTGGGGAGCATCAAGTCTGGTTGGCGTAGTGTTTTCATGATTTTTACAGCTTCTGCAGATGATGAATTGGAATCTATTGTTGAAAGTGCATTTGAAAATGTCGAACAGGTGATCTTGGAACACTTTGATCAAGTTGTTGGAGATTGTTTTATGGACTGTGTCAACTGTCTTATTAGATTTGCAAATAATAGAACTTCACACCGTATAAGTTTGAAGGCTATTGCACTCCTGCGCATATGTGAGGATCGTTTGGCTGAGGGTCTTATACCTGGTGGTGCTTTGAAGCCTATAGAAGAAAATGAGACTACAACTTTTGATGTGACCGAGCATTATTGGTTCCCAATGCTGGCTGGTCTGTCTGATCTGACATCAGATACAAGACCAGAAGTCAGAAGCTGTGCGCTTGAAGTTTTGTTTGATCTACTGAATGAGAGAGGTACCAAGTTCTCGTCATCCTTTTGGGAGAGCATCTTCCATCGTGTCTTGTTTCCCATATTTGATCATGTAAGAAATGCTGGAAAGGAAAGCTCGACTTCTTCTGATGAGGAATGGTTCCGTGAAACAAGCATTCACTCACTTCAGTTGCTGTGCAACCTTTTCAACACTTTTTATAAGGAGGTGTGTTTTATGTTGCCACCACTACTCAGTCTACTGTTAGATTGTGCCAAACAGACTGATCAAGCAGTGGTTTCGTTATCTCTGGGTGCACTGGTTCATCTCATTGAAGTTGGAGGACATCAATTTAGTGAGAGTGATTGGGATACATTATTGAAAAGCATAAGAGATGCTTTGTACACAACACAACCACTTGAACTGCTAAATGCTTTGGGTTTTGAGAACCTGAAGAATGATAGGACATTAAATTTAGAGGTCAATACCGGTGATGGTGCTTCCTTAATGCCTGTTGACTATGATGGGGGGGACAACCGTGAGTTTGATGCCAATGATAGAAATCCAAATGCATCAGTCGAATCAGGGGCGCAAATGAACTTGGATGGATCTGAAGGCCTCAACTCACCATCAGGATCTGCTTCAAAATCTGCTGAAGATGGAAACCTCCAACGTAGTCAAACAATAGGTCAAAGAATTATGGATAACCTCTTTCTCAGAAATCTTTCATCTAAGCCTAAGAGTTCTGATGCTTCAGTACCATCTACTCCAGTTAAGGTTCCTGATGCTGTCGAGCTTGATACCAAAGATGAGGAGGAAAGCTCTGTATTGGGAACTTGTAGGGGCAAATGCATTACTCAATTATTACTTCTGGGGGCCATTGATAGTATCCAGAAGAAATACTGGAGCAAGTTGAAGGCACCACAGAAGATTGCTATAATGGACATTTTGCTGTCCGCGTTAGAATTTGCAGCTTCCTACAATTCATATACCAATCTCAGGACACGCATGCACCAAATTTCTGATGAGAGGCCACCTCTGAATCTTCTTCGCCAAGAGTTAACAGGAACTTGCATATATCTGGACATCTTACAGAAAGCAACTTCCGGGTTTAGTGCCAATCAAGAGGGAAATGATGAAGAGAAAGTTGAAGGATTGGCAGAAGACAAACTGGTGTCTTTCTGCGAACAGGTACTCAGGGAGGCATCCGAGCTGCAGTCCAGTTCTGGAGACGTGACTAATATGGATATTCATCGTGTTCTGGAGTTACGATCTGCAATAATTGTTAAGGTGCTCAAAGGCATGTGCTTTATGAACCAACAGGTTTTCAGAAGACATCTGAGAGACTTCTATCCTTTGCTTACAAAACTTGTATGCTGCGACCAGATGGATGTTCGTGGAGCTCTTGGTGATCTATTTAGGGCACAATTGAAAGCACTTCTGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1992

Amino Acids

221.2

Weight (kDa)

5.4

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 103 - 161 3.8e-08 Ring finger domain
zf-RING_11 PF17123 105 - 133 1.2e-06 RING-like zinc finger
DCB PF16213 362 - 495 1e-25 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 591 - 743 4.1e-41 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 838 - 1019 9.6e-71 Sec7 domain
Sec7-like_HDS PF09324 1332 - 1414 2.5e-26 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1488 - 1562 4.3e-08 C-terminal region of Mon2 protein
BIG2_C PF20252 1795 - 1982 1.4e-26 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4749
AarI CACCTGC 2 cut(s) 320, 1652
AatII GACGTC 2 cut(s) 60, 500
Acc16I TGCGCA 4 cut(s) 2925, 2972, 4070, 4370
Acc36I ACCTGC 4 cut(s) 320, 1156, 1652, 3386
Acc65I GGTACC 1 cut(s) 4574
AccB1I GGYRCC 4 cut(s) 443, 2218, 4574, 5446
AccB7I CCANNNNNTGG 1 cut(s) 947
AccBSI CCGCTC 2 cut(s) 631, 2236
AccI GTMKAC 5 cut(s) 227, 2516, 3400, 3874, 4786
AccII CGCG 4 cut(s) 86, 576, 648, 5486
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 7 cut(s) 1534, 2013, 2687, 2729, 4999, 5492, 5544
AcvI CACGTG 1 cut(s) 2387
AcyI GRCGYC 4 cut(s) 57, 444, 485, 497
AdeI CACNNNGTG 3 cut(s) 393, 872, 3782
AflII CTTAAG 1 cut(s) 4046
AflIII ACRYGT 2 cut(s) 207, 3412
AgeI ACCGGT 1 cut(s) 5015
AhdI GACNNNNNGTC 2 cut(s) 3131, 4523
AhlI ACTAGT 1 cut(s) 3077
AjiI CACGTC 4 cut(s) 210, 379, 468, 5761
AjuI GAANNNNNNNTTGG 8 cut(s) 2948, 2980, 3566, 3598, 4013, 4026, 4045, 4058
AloI GAACNNNNNNTCC 2 cut(s) 579, 611
Alw21I GWGCWC 8 cut(s) 779, 877, 3007, 3217, 3244, 4846, 5823, 5932
Alw44I GTGCAC 1 cut(s) 4842
Ama87I CYCGRG 2 cut(s) 3083, 3819
ApaI GGGCCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 4842
ApoI RAATTY 7 cut(s) 1534, 2013, 2687, 2729, 4999, 5492, 5544
ArsI GACNNNNNNTTYG 2 cut(s) 5200, 5232
AsiGI ACCGGT 1 cut(s) 5015
Asp700I GAANNNNTTC 4 cut(s) 1036, 2688, 3709, 5251
Asp718I GGTACC 1 cut(s) 4574
AsuC2I CCSGG 2 cut(s) 356, 5635
AvaI CYCGRG 2 cut(s) 3083, 3819
AvaII GGWCC 5 cut(s) 40, 352, 1309, 2094, 3555
BaeGI GKGCMC 4 cut(s) 115, 473, 4846, 5953
BamHI GGATCC 1 cut(s) 2167
BanI GGYRCC 4 cut(s) 443, 2218, 4574, 5446
BanII GRGCYC 2 cut(s) 115, 5932
BarI GAAGNNNNNNTAC 2 cut(s) 196, 228
BbrPI CACGTG 1 cut(s) 2387
BbsI GAAGAC 9 cut(s) 27, 204, 373, 662, 1853, 3165, 3247, 5697, 5866
Bbv12I GWGCWC 8 cut(s) 779, 877, 3007, 3217, 3244, 4846, 5823, 5932
BbvCI CCTCAGC 2 cut(s) 362, 4392
BceAI ACGGC 8 cut(s) 302, 436, 457, 1676, 1691, 1966, 3614, 5956
BcgI CGANNNNNNTGC 4 cut(s) 1785, 1819, 4809, 4843
BciVI GTATCC 3 cut(s) 3421, 4891, 5426
BclI TGATCA 6 cut(s) 237, 427, 1452, 4264, 4636, 4813
BcnI CCSGG 2 cut(s) 356, 5635
BcuI ACTAGT 1 cut(s) 3077
BfaI CTAG 8 cut(s) 543, 1049, 1142, 1911, 2607, 3078, 3530, 3891
BfmI CTRYAG 8 cut(s) 478, 897, 1320, 1874, 3756, 4191, 4425, 5741
BfoI RGCGCY 2 cut(s) 447, 1080
BfrI CTTAAG 1 cut(s) 4046
BfuAI ACCTGC 4 cut(s) 320, 1156, 1652, 3386
BfuI GTATCC 3 cut(s) 3421, 4891, 5426
BglI GCCNNNNNGGC 1 cut(s) 995
BmcAI AGTACT 2 cut(s) 2635, 3076
Bme18I GGWCC 5 cut(s) 40, 352, 1309, 2094, 3555
BmeRI GACNNNNNGTC 2 cut(s) 3131, 4523
BmeT110I CYCGRG 2 cut(s) 3083, 3819
BmgBI CACGTC 4 cut(s) 210, 379, 468, 5761
BmrI ACTGGG 4 cut(s) 118, 2634, 3561, 3741
BmuI ACTGGG 4 cut(s) 118, 2634, 3561, 3741
BpiI GAAGAC 9 cut(s) 27, 204, 373, 662, 1853, 3165, 3247, 5697, 5866
BplI GAGNNNNNCTC 6 cut(s) 2034, 2066, 5333, 5365, 5548, 5580
BpmI CTGGAG 7 cut(s) 606, 1772, 4022, 5280, 5452, 5775, 5810
Bpu10I CCTNAGC 5 cut(s) 326, 362, 518, 1080, 4392
BpuEI CTTGAG 4 cut(s) 1886, 2036, 2234, 2250
BpuMI CCSGG 2 cut(s) 356, 5635
BsaAI YACGTR 1 cut(s) 2387
BsaBI GATNNNNATC 4 cut(s) 153, 1457, 5088, 5778
BsaHI GRCGYC 4 cut(s) 57, 444, 485, 497
BsaI GGTCTC 2 cut(s) 1472, 2427
BsaJI CCNNGG 5 cut(s) 574, 990, 2097, 2789, 3622
BsaWI WCCGGW 4 cut(s) 1225, 1486, 2091, 5015
BsaXI ACNNNNNCTCC 4 cut(s) 199, 229, 579, 609
Bse118I RCCGGY 2 cut(s) 731, 5015
Bse3DI GCAATG 3 cut(s) 1173, 1443, 3515
Bse8I GATNNNNATC 4 cut(s) 153, 1457, 5088, 5778
BseDI CCNNGG 5 cut(s) 574, 990, 2097, 2789, 3622
BseJI GATNNNNATC 4 cut(s) 153, 1457, 5088, 5778
BseMI GCAATG 3 cut(s) 1173, 1443, 3515
BseRI GAGGAG 4 cut(s) 284, 1007, 3359, 5354
BseSI GKGCMC 4 cut(s) 115, 473, 4846, 5953
BseYI CCCAGC 5 cut(s) 231, 522, 1058, 1363, 3929
BsgI GTGCAG 4 cut(s) 972, 1382, 2865, 3759
Bsh1236I CGCG 4 cut(s) 86, 576, 648, 5486
BshNI GGYRCC 4 cut(s) 443, 2218, 4574, 5446
BshTI ACCGGT 1 cut(s) 5015
BsiHKAI GWGCWC 8 cut(s) 779, 877, 3007, 3217, 3244, 4846, 5823, 5932
BsiHKCI CYCGRG 2 cut(s) 3083, 3819
BsiSI CCGG 8 cut(s) 127, 355, 732, 1226, 1487, 2092, 5016, 5634
BsiWI CGTACG 1 cut(s) 1179
BslFI GGGAC 7 cut(s) 320, 359, 1969, 2080, 2284, 3541, 5072
BsmBI CGTCTC 2 cut(s) 274, 5751
BsmFI GGGAC 7 cut(s) 320, 359, 1969, 2080, 2284, 3541, 5072
BsmI GAATGC 4 cut(s) 1115, 2983, 3580, 4704
Bso31I GGTCTC 2 cut(s) 1472, 2427
BsoBI CYCGRG 2 cut(s) 3083, 3819
Bsp120I GGGCCC 1 cut(s) 111
Bsp1407I TGTACA 1 cut(s) 4929
BspFNI CGCG 4 cut(s) 86, 576, 648, 5486
BspHI TCATGA 4 cut(s) 1714, 2680, 3940, 4173
BspMAI CTGCAG 5 cut(s) 482, 1324, 1878, 4195, 5745
BspMI ACCTGC 4 cut(s) 320, 1156, 1652, 3386
BspQI GCTCTTC 1 cut(s) 3159
BspT107I GGYRCC 4 cut(s) 443, 2218, 4574, 5446
BspTI CTTAAG 1 cut(s) 4046
BspTNI GGTCTC 2 cut(s) 1472, 2427
BsrBI CCGCTC 2 cut(s) 631, 2236
BsrDI GCAATG 3 cut(s) 1173, 1443, 3515
BsrFI RCCGGY 2 cut(s) 731, 5015
BsrGI TGTACA 1 cut(s) 4929
BssAI RCCGGY 2 cut(s) 731, 5015
BssECI CCNNGG 5 cut(s) 574, 990, 2097, 2789, 3622
BssNAI GTATAC 2 cut(s) 3401, 3875
BssNI GRCGYC 4 cut(s) 57, 444, 485, 497
Bst1107I GTATAC 2 cut(s) 3401, 3875
Bst6I CTCTTC 7 cut(s) 202, 2340, 2867, 3045, 3111, 3159, 5661
BstACI GRCGYC 4 cut(s) 57, 444, 485, 497
BstAFI CTTAAG 1 cut(s) 4046
BstAPI GCANNNNNTGC 2 cut(s) 1667, 2879
BstAUI TGTACA 1 cut(s) 4929
BstBAI YACGTR 1 cut(s) 2387
BstDSI CCRYGG 1 cut(s) 574
BstENI CCTNNNNNAGG 2 cut(s) 65, 688
BstFNI CGCG 4 cut(s) 86, 576, 648, 5486
BstH2I RGCGCY 2 cut(s) 447, 1080
BstNSI RCATGY 5 cut(s) 2711, 3416, 3482, 5539, 5833
BstSFI CTRYAG 8 cut(s) 478, 897, 1320, 1874, 3756, 4191, 4425, 5741
BstSLI GKGCMC 4 cut(s) 115, 473, 4846, 5953
BstUI CGCG 4 cut(s) 86, 576, 648, 5486
BstV2I GAAGAC 9 cut(s) 27, 204, 373, 662, 1853, 3165, 3247, 5697, 5866
BstX2I RGATCY 7 cut(s) 507, 1198, 2167, 2284, 2989, 5135, 5162
BstXI CCANNNNNNTGG 1 cut(s) 2940
BstYI RGATCY 7 cut(s) 507, 1198, 2167, 2284, 2989, 5135, 5162
BstZ17I GTATAC 2 cut(s) 3401, 3875
BsuI GTATCC 3 cut(s) 3421, 4891, 5426
BtgI CCRYGG 1 cut(s) 574
BtgZI GCGATG 1 cut(s) 486
BtrI CACGTC 4 cut(s) 210, 379, 468, 5761
BtsI GCAGTG 4 cut(s) 984, 1329, 3771, 4827
BveI ACCTGC 4 cut(s) 320, 1156, 1652, 3386
CciI TCATGA 4 cut(s) 1714, 2680, 3940, 4173
Cfr10I RCCGGY 2 cut(s) 731, 5015
Cfr42I CCGCGG 1 cut(s) 577
CseI GACGC 2 cut(s) 493, 2281
CsiI ACCWGGT 3 cut(s) 958, 1611, 4405
CspAI ACCGGT 1 cut(s) 5015
CspCI CAANNNNNGTGG 2 cut(s) 2473, 2508
DinI GGCGCC 1 cut(s) 445
DraI TTTAAA 1 cut(s) 3391
DraIII CACNNNGTG 3 cut(s) 393, 872, 3782
DriI GACNNNNNGTC 2 cut(s) 3131, 4523
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 7 cut(s) 202, 2340, 2867, 3045, 3111, 3159, 5661
Eam1105I GACNNNNNGTC 2 cut(s) 3131, 4523
EarI CTCTTC 7 cut(s) 202, 2340, 2867, 3045, 3111, 3159, 5661
EciI GGCGGA 2 cut(s) 113, 1100
Ecl136II GAGCTC 1 cut(s) 5930
Eco147I AGGCCT 3 cut(s) 2859, 3289, 5145
Eco24I GRGCYC 2 cut(s) 115, 5932
Eco31I GGTCTC 2 cut(s) 1472, 2427
Eco47I GGWCC 5 cut(s) 40, 352, 1309, 2094, 3555
Eco53kI GAGCTC 1 cut(s) 5930
Eco72I CACGTG 1 cut(s) 2387
Eco88I CYCGRG 2 cut(s) 3083, 3819
EcoICRI GAGCTC 1 cut(s) 5930
EcoNI CCTNNNNNAGG 2 cut(s) 65, 688
EcoO109I RGGNCCY 4 cut(s) 112, 1309, 3555, 4096
EcoT22I ATGCAT 3 cut(s) 2709, 5101, 5381
EcoT38I GRGCYC 2 cut(s) 115, 5932
EgeI GGCGCC 1 cut(s) 445
EheI GGCGCC 1 cut(s) 445
Esp3I CGTCTC 2 cut(s) 274, 5751
FalI AAGNNNNNCTT 6 cut(s) 59, 91, 4654, 4686, 4909, 4941
FaqI GGGAC 7 cut(s) 320, 359, 1969, 2080, 2284, 3541, 5072
FauI CCCGC 3 cut(s) 581, 712, 1959
FauNDI CATATG 3 cut(s) 1954, 2896, 4373
FbaI TGATCA 6 cut(s) 237, 427, 1452, 4264, 4636, 4813
FblI GTMKAC 5 cut(s) 227, 2516, 3400, 3874, 4786
FriOI GRGCYC 2 cut(s) 115, 5932
FspAI RTGCGCAY 2 cut(s) 2925, 2972
FspBI CTAG 8 cut(s) 543, 1049, 1142, 1911, 2607, 3078, 3530, 3891
FspI TGCGCA 4 cut(s) 2925, 2972, 4070, 4370
GsaI CCCAGC 5 cut(s) 235, 526, 1062, 1367, 3933
GsuI CTGGAG 7 cut(s) 606, 1772, 4022, 5280, 5452, 5775, 5810
HaeII RGCGCY 2 cut(s) 447, 1080
HapII CCGG 8 cut(s) 127, 355, 732, 1226, 1487, 2092, 5016, 5634
HgaI GACGC 2 cut(s) 493, 2281
Hin1I GRCGYC 4 cut(s) 57, 444, 485, 497
HincII GTYRAC 8 cut(s) 228, 1267, 1417, 2185, 4108, 4300, 5044, 5589
HindII GTYRAC 8 cut(s) 228, 1267, 1417, 2185, 4108, 4300, 5044, 5589
HpaI GTTAAC 1 cut(s) 5589
HpaII CCGG 8 cut(s) 127, 355, 732, 1226, 1487, 2092, 5016, 5634
Hpy99I CGWCG 6 cut(s) 59, 146, 226, 229, 265, 648
Hsp92I GRCGYC 4 cut(s) 57, 444, 485, 497
KasI GGCGCC 1 cut(s) 443
KflI GGGWCCC 1 cut(s) 3555
KpnI GGTACC 1 cut(s) 4578
KroI GCCGGC 1 cut(s) 731
KroNI GCCGGC 1 cut(s) 733
Ksp22I TGATCA 6 cut(s) 237, 427, 1452, 4264, 4636, 4813
KspAI GTTAAC 1 cut(s) 5589
KspI CCGCGG 1 cut(s) 577
LguI GCTCTTC 1 cut(s) 3159
MabI ACCWGGT 3 cut(s) 958, 1611, 4405
MaeI CTAG 8 cut(s) 543, 1049, 1142, 1911, 2607, 3078, 3530, 3891
MbiI CCGCTC 2 cut(s) 631, 2236
MfeI CAATTG 1 cut(s) 5954
MflI RGATCY 7 cut(s) 507, 1198, 2167, 2284, 2989, 5135, 5162
Mly113I GGCGCC 1 cut(s) 444
Mph1103I ATGCAT 3 cut(s) 2709, 5101, 5381
MroNI GCCGGC 1 cut(s) 731
MroXI GAANNNNTTC 4 cut(s) 1036, 2688, 3709, 5251
MslI CAYNNNNRTG 4 cut(s) 1719, 2192, 2712, 3942
MspA1I CMGCKG 5 cut(s) 235, 551, 576, 3794, 3929
MspCI CTTAAG 1 cut(s) 4046
MspI CCGG 8 cut(s) 127, 355, 732, 1226, 1487, 2092, 5016, 5634
MunI CAATTG 1 cut(s) 5954
Mva1269I GAATGC 4 cut(s) 1115, 2983, 3580, 4704
MvnI CGCG 4 cut(s) 86, 576, 648, 5486
NaeI GCCGGC 1 cut(s) 733
NarI GGCGCC 1 cut(s) 444
NciI CCSGG 2 cut(s) 356, 5635
NdeI CATATG 3 cut(s) 1954, 2896, 4373
NgoMIV GCCGGC 1 cut(s) 731
NmeAIII GCCGAG 1 cut(s) 1015
NmuCI GTSAC 7 cut(s) 274, 742, 2382, 2557, 3539, 4456, 5761
NsbI TGCGCA 4 cut(s) 2925, 2972, 4070, 4370
NsiI ATGCAT 3 cut(s) 2709, 5101, 5381
NspI RCATGY 5 cut(s) 2711, 3416, 3482, 5539, 5833
PaeI GCATGC 3 cut(s) 2711, 3482, 5539
PaeR7I CTCGAG 1 cut(s) 3083
PagI TCATGA 4 cut(s) 1714, 2680, 3940, 4173
PaqCI CACCTGC 2 cut(s) 320, 1652
PceI AGGCCT 3 cut(s) 2859, 3289, 5145
PciI ACATGT 1 cut(s) 3412
PciSI GCTCTTC 1 cut(s) 3159
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 673
PctI GAATGC 4 cut(s) 1115, 2983, 3580, 4704
PdiI GCCGGC 1 cut(s) 733
PdmI GAANNNNTTC 4 cut(s) 1036, 2688, 3709, 5251
Pfl23II CGTACG 1 cut(s) 1179
PflFI GACNNNGTC 4 cut(s) 350, 3169, 3553, 4295
PflMI CCANNNNNTGG 1 cut(s) 947
PfoI TCCNGGA 2 cut(s) 354, 1695
PinAI ACCGGT 1 cut(s) 5015
PluTI GGCGCC 1 cut(s) 447
PmaCI CACGTG 1 cut(s) 2387
PmlI CACGTG 1 cut(s) 2387
Ppu21I YACGTR 1 cut(s) 2387
PpuMI RGGWCCY 2 cut(s) 1309, 3555
PscI ACATGT 1 cut(s) 3412
PsiI TTATAA 1 cut(s) 4749
Psp124BI GAGCTC 1 cut(s) 5932
Psp5II RGGWCCY 2 cut(s) 1309, 3555
PspCI CACGTG 1 cut(s) 2387
PspFI CCCAGC 5 cut(s) 231, 522, 1058, 1363, 3929
PspLI CGTACG 1 cut(s) 1179
PspOMI GGGCCC 1 cut(s) 111
PspPPI RGGWCCY 2 cut(s) 1309, 3555
PstI CTGCAG 5 cut(s) 482, 1324, 1878, 4195, 5745
PsuI RGATCY 7 cut(s) 507, 1198, 2167, 2284, 2989, 5135, 5162
PsyI GACNNNGTC 4 cut(s) 350, 3169, 3553, 4295
PvuII CAGCTG 2 cut(s) 235, 3929
RseI CAYNNNNRTG 4 cut(s) 1719, 2192, 2712, 3942
SacI GAGCTC 1 cut(s) 5932
SacII CCGCGG 1 cut(s) 577
SalI GTCGAC 1 cut(s) 226
SapI GCTCTTC 1 cut(s) 3159
ScaI AGTACT 2 cut(s) 2635, 3076
SexAI ACCWGGT 3 cut(s) 958, 1611, 4405
SfcI CTRYAG 8 cut(s) 478, 897, 1320, 1874, 3756, 4191, 4425, 5741
SfoI GGCGCC 1 cut(s) 445
Sfr274I CTCGAG 1 cut(s) 3083
Sfr303I CCGCGG 1 cut(s) 577
SgrBI CCGCGG 1 cut(s) 577
SinI GGWCC 5 cut(s) 40, 352, 1309, 2094, 3555
SlaI CTCGAG 1 cut(s) 3083
SmiMI CAYNNNNRTG 4 cut(s) 1719, 2192, 2712, 3942
SmlI CTYRAG 6 cut(s) 1865, 2051, 2213, 2265, 3083, 4046
SmoI CTYRAG 6 cut(s) 1865, 2051, 2213, 2265, 3083, 4046
SpeI ACTAGT 1 cut(s) 3077
SphI GCATGC 3 cut(s) 2711, 3482, 5539
SseBI AGGCCT 3 cut(s) 2859, 3289, 5145
SspDI GGCGCC 1 cut(s) 443
SspI AATATT 2 cut(s) 1390, 2669
SspMI CTAG 8 cut(s) 543, 1049, 1142, 1911, 2607, 3078, 3530, 3891
SstI GAGCTC 1 cut(s) 5932
StuI AGGCCT 3 cut(s) 2859, 3289, 5145
TaqII GACCGA 3 cut(s) 1496, 2409, 4475
TatI WGTACW 5 cut(s) 187, 1345, 2633, 3074, 4929
TauI GCSGC 2 cut(s) 74, 738
TseFI GTSAC 7 cut(s) 274, 742, 2382, 2557, 3539, 4456, 5761
Tsp45I GTSAC 7 cut(s) 274, 742, 2382, 2557, 3539, 4456, 5761
TspGWI ACGGA 4 cut(s) 182, 271, 1924, 4682
Tth111I GACNNNGTC 4 cut(s) 350, 3169, 3553, 4295
Van91I CCANNNNNTGG 1 cut(s) 947
Vha464I CTTAAG 1 cut(s) 4046
VneI GTGCAC 1 cut(s) 4842
VpaK11BI GGWCC 5 cut(s) 40, 352, 1309, 2094, 3555
XagI CCTNNNNNAGG 2 cut(s) 65, 688
XapI RAATTY 7 cut(s) 1534, 2013, 2687, 2729, 4999, 5492, 5544
XbaI TCTAGA 1 cut(s) 1141
XceI RCATGY 5 cut(s) 2711, 3416, 3482, 5539, 5833
XhoI CTCGAG 1 cut(s) 3083
XmiI GTMKAC 5 cut(s) 227, 2516, 3400, 3874, 4786
XmnI GAANNNNTTC 4 cut(s) 1036, 2688, 3709, 5251
XspI CTAG 8 cut(s) 543, 1049, 1142, 1911, 2607, 3078, 3530, 3891
ZraI GACGTC 2 cut(s) 58, 498
ZrmI AGTACT 2 cut(s) 2635, 3076
Zsp2I ATGCAT 3 cut(s) 2709, 5101, 5381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.