Rh2DG108400

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007281
Physical Location & Seq
Reverse (-)
8918720 .. 8931147
12428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG108400.1

Sequence Viewer

Length: 5130 bp
ATGGCGGGAGGCGCAGCCGGCGGCTTCGTGACGAGAGCCTATGAGTCCATGCTCAAAGAGTGCTCACCGAAAAAGTATTCCGATCTTCAGAAGGCAATTCAGGCTTATTTAGACAATGTGAAAGCGGTTAATCAGGCTGAACAAACCACACCACAAACCGCTCCAAGTGAGAAGAATCCAGCTACAGATGGTGATGGAAGTTCTCTTGAAACAGAAGGTGAAGCTCCAAAAACTGATGCAGAACCAGGTCAGTCTCAAACATCCTCACACACTGCCGAGGAGGCAGATTCTGTTGGCAGGCCAGTGAGCATAAGTGGAACTGTTTCAACTGTCCTAGCAACTGCTGGGAACACCTTAGAAGGCGCTCAGGCGGAGCTTGTTTTGAATCCCCTGCGACTTGCATTTGATACAAAGAACTTGAAAGTTCTAGAACCTGCTCTGGATTGTTTTCATAAACTCATTGCGTACGATCATTTAGAGGGAGATCCTGGTTTAGACGATAAAAGTGTTCCGGTGTTTACCGACATTTTGAACAGGGTTTGCAGTTGTGTTGACAATAATTCACCTGATAGTACTGTTCTGCAAGTGTTGAAGGTCCTTCTTACTGCAGTGGCATCCACAAAGTTTAGAGTACATGGGGAACCTTTGCTGGGAGTTATCAGAGTGTGCTATAATATTGCTCTGCACAGCAAGAGTCCTGTCAACCAAGCTACATCAAAGGCAATGCTTACGCAGATGATCAGCATCATATTCAGGAGAATGGAGACCGATCCGGTTTCATCATCTGGTTCTGTTGGACAAAATACAAATACAGCACAAAATTCAAATACAGAAGCTGAAGAAACTTCTGTGGCAGACCAAAATGAGAAAGGAATGACTTTAGGAGATCAACTTAACCAGGTTAAAGAGACACCCATTGCATCTGTTGAGGAACTTCATAATCTTGCAGGTGGTGCTGACATCAAGGGTTTAGAGGCCGTCCTGGACAAAGCCGTGCATCATGAAGATGGCAAAAAGATAACCAGAGGGATTGACCTGGAGAGTATGAGCATAGTTCAACGTGATGCTTTACTGGTTTTCCGAACCCTGTGCAAGATGGGCATGAAGGAAGATAACAATGAAGTTACGTTGAAGACGAGGATTTTATCTCTTGAGCTTCTGCAGGGTTTGTTGGAAGGAGTTGGTCATCCATTTACTAGGAACTTCCATTTTATTGATTCCGTGAAAGCATACCTATCATATGCTTTGTTGCGGGCTTCAGTTTCACCGTCCCCTATCATATTTCAGTATGCAAGTGGAATTTTTTTAGTGCTTTTGTTGCGGTTTAGAGAGAGTCTCAAGGGTGAAATAGGCATCTTCTTTCCCTTGATAGTTCTCCGGTCCCTGGATGGCTCGGATTTACCCATGAACCAGAAAATGAGTGTTCTTAGGATGTTAGAAAAAGTTTGCAAGGATCCTCAGATGCTTGTTGACATATTTGTGAACTATGACTGTGATCTTGAGGCACCGAACTTGTTTGAGCGGATGGTTACAACTCTATCCAGAATATCTCAAGGGACACAAAATGCAGATCCGAATATGGCTGGTGCATCCCAGACTACTTCAATAAAGGGTTCATCTCTTCAGTGTCTTGTGAATGTGCTTAAATCACTCGTAGATTGGGAGAAGTCACGTGGAGAATCAGATAGCCATAGTAAAAACACTCGGTCTCTGGAAGGAGATGCTTCAGCTAAAGAGTCTGTTGATGTGAAAAGCCGGCAAGATACAACCACTAACTTTGAGAAGGCAAAAGCTCATAAGTCTACACTGGAAGCTGCCATATCTGAGTTCAACAGGCGACCAGTGAAGGGTGTAGAGTATCTGAGATCAAATAAATTAGTGGAAAACACACCTAGTTCAGTTGCCCAATTTTTGAAAAGTACTCCCAGTTTGGACAAGGCTATGATTGGGGAATATTTGGGCCATCATGAAGAATTTCCTCTTTCTGTTATGCATGCTTATGTAGATTCCATGAAATTTTCGGGAATGAAGTTTGATACTGCAATTCGTGAACTTCTTAAAGGATTCCGACTTCCAGGGGAAGCTCAAAAGATAGATCGCATCATGGAAAAGTTTGCAGAAAGATACTGTGCAGATAATCCAGGCCTCTTCAAAAATGCAGATACTGCTTACGTTCTTGCATATGCAGTTATTATGTTGAATACTGATGCGCATAATCCAATGGTGTGGCCTAAGATGTCCAAGTCAGATTTCGTGCGCATGAATGCCACTGAAGATCCAGAAGATTGTGCTCCAACTGAACTTCTGGAAGAAATCTATGACTCAATTATTAAAGAAGAGATAAAGATGAAAGATGAGAGTACTAGTCTCGAGAAAAGTGGCAAATATAAGCCAGAAGGTGAAGAGAGAGGACGACTTGTCAGTATTCTTAATCTGGCTCTTCCCAGAAGGACACTGTCTTCTGATACCAAGTCTGAAAGTGAAGCTATCATAAAGAGAGCACAAGATATATTTAGGAATCAAGGAGCACAAAGAGGGGTCTTCCATACTACACAGCAGATAGAACTTGTAAGGCCTATGGTAGAAGCTGTTGGATGGCCTTTGCTTGCTACCTTCTCTGTTACAATGGAGGAGGGTGAGAATAAGTCGAGGGTTGTTCTCTGCATGGAAGGATTTAAAGCAGGAATACACATTACACATGTTCTGGGGATGGATACCATGCGCTATGCCTTCTTAACATCTCTGGTCAGATTTACATTCTTGCATGCTCCAAAGGAAATGCGTAGTAAAAATGTGGAAGCATTGCGAACTCTACTAGCTCTTTGTGACTCAGAGACTGGGTCCCTTCAAGACACATGGAATGCAGTTTTGGAATGTGTTTCACGGCTTGAATACATCACTTCAACCCCTGGTATTGCTGCAACAGTCATGCAGGGATCAAACCAAATCTCTAAAGATGCTGTTCTTCAATCTCTAAGAGAATTGGCTGGAAAACCTTCTGAGCAAGTATTTGTGAATAGTGTTCAGCTTCCCAGTGATTCTGTAGTGGAGTTTTTCACTGCACTCTGTGGTGTATCAGCGGAAGAACTAAAACAAAGTCCAGCTCGGGTTTTTAGCTTGCAGAAACTTGTTGAGATCAGCTATTACAACATGGCTCGTATACGCATGGTCTGGGCTAGGATATGGTCTGTTTTGGCAAACCACTTTATTTCAGCTGGGAGTCATCATGATGAGAAAATTGCTATGTATGCCATAGATTCTCTAAGGCAACTTGGTATGAAGTATCTGGAGCGAGCTGAACTTGCCAATTTCACATTCCAAAATGACATTCTTAAGCCTTTTGTTGTTCTTATGCGCAATAGTCGAAGTGAAACCATAAGGGGCCTAATTGTTGACTGCATTGTTCAAATGATAAAATCTAAAGTGGGGAGCATCAAGTCTGGTTGGCGTAGTGTTTTCATGATTTTTACAGCTTCTGCTGATGATGAATTGGAATCTATTGTTGAAAGTGCATTTGAAAATGTCGAACAGGTGATCTTGGAACACTTTGATCAAGTTGTTGGAGATTGTTTTATGGACTGTGTCAACTGCCTTATTAGATTTGCAAATAATAGAACTTCACACCGTATAAGTTTGAAGGCTATTGCACTCCTGCGCATATGTGAGGATCGTTTGGCTGAGGGTCTTATACCTGGTGGTGCTTTGAAGCCTATAGAAGAAAATGAGACTACAACTTTTGATGTGACCGAGCATTATTGGTTCCCAATGCTGGCTGGTCTGTCTGATCTGACATCAGATACAAGACCAGAAGTCAGAAGCTGTGCGCTTGAAGTTTTGTTTGATCTACTGAATGAGAGAGGTACCAAGTTCTCGTCATCCTTTTGGGAGAGCATCTTCCATCGTGTCTTGTTTCCCATATTTGATCATGTAAGAAATGCTGGAAAGGAAAGCTCGACTTCTTCTGATGAGGAATGGTTCCGTGAAACAAGCATTCACTCACTTCAGTTGCTGTGCAACCTTTTCAACACTTTTTATAAGGAGGTATGTTTTATGTTGCCACCACTACTCAGTCTACTGTTAGATTGTGCTAAACAGACTGATCAAGCAGTGGTTTCGTTATCTCTGGGTGCACTGGTTCATCTCATTGAAGTTGGAGGACATCAATTTAGTGAGAGTGATTGGGATACGTTATTGAAAAGCATAAGAGATGCTTTGTACACAACACAACCACTTGAACTGCTAAATGCTTTGGGTTTTGAGAACCTGAAGAATGATAGGACATTAAATGTAGAGGTCAATACCGGTGATGGTGCTTCCTTAATGCCTGTTGACTATGATGGGGGGGACAACCGTCAGTTTGATGCCAATGATAGAAATCCAAATGCATCAGTCGAATCAGGGGCGCAAATGAACTTGGATGGATCTGAAGGCCTCAACTCACCATCAGGATCTGCTTCAAAATCTGCTGAAGATGGAAACCTCCAACGTAGTCAAACAATAGGTCAAAGAATTATGGATAACCTCTTTCTCAGAAATCTTTCATCTAAGCCTAAGAGTTCTGATGCTTCAGTACCATCTACTCCAGTTAAGGTTCCTGATGCTGTCGAGCCTGATACCAAAGATGAGGAGGAAAGCTCTGTATTGGGAACTTGTAGGGGCAAATGCATTACTCAATTATTACTTCTGGGGGCCATTGATAGTATCCAGAAGAAATACTGGAGCAAGTTGAAGGCACCACAGAAGATTGCTATAATGGACATTTTGCTGTCCGCGTTAGAATTTGCAGCTTCCTACAATTCATATACAAATCTCAGGACACGCATGCACCAAATTTCTGATGAGAGGCCACCTCTGAATCTTCTTCGCCAAGAGTTAACAGGAACTTGCATATATCTGGACATCTTACAGAAAGCAACTTCCGGGTTTAGTGCCAATCAAGAGGGAAATGATGAAGAGAAAGTTGAAGGATTGGCAGAAGACAAACTGGTGTCTTTCTGTGAACAGGTACTCAGGGAGGCATCCGAGCTGCAGTCCAGTTCTGGAGATGTGACTAATATGGATATTCACCGTGTTCTGGAGTTACGATCTCCAATAATTGTTAAGTTCCAGCAGCTGCCTTTTATTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1709

Amino Acids

189.22

Weight (kDa)

5.23

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 124 - 257 7.9e-26 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 353 - 505 6.8e-41 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 600 - 781 2.1e-71 Sec7 domain
Sec7-like_HDS PF09324 1094 - 1176 2.1e-26 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1250 - 1324 3.5e-08 C-terminal region of Mon2 protein
BIG2_C PF20252 1557 - 1702 1.3e-13 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4035
AarI CACCTGC 1 cut(s) 938
Acc16I TGCGCA 4 cut(s) 2211, 2258, 3356, 3656
Acc36I ACCTGC 2 cut(s) 442, 938
Acc65I GGTACC 1 cut(s) 3860
AccB1I GGYRCC 3 cut(s) 1504, 3860, 4732
AccBSI CCGCTC 2 cut(s) 161, 1522
AccI GTMKAC 3 cut(s) 1802, 3160, 4072
AccII CGCG 1 cut(s) 4772
AcsI RAATTY 6 cut(s) 820, 1299, 1973, 2015, 4778, 4830
AcvI CACGTG 1 cut(s) 1673
AdeI CACNNNGTG 1 cut(s) 3068
AfaI GTAC 9 cut(s) 467, 574, 633, 1921, 2362, 3862, 4217, 4572, 5007
AfiI CCNNNNNNNGG 5 cut(s) 650, 1384, 1847, 3769, 5074
AflII CTTAAG 1 cut(s) 3332
AflIII ACRYGT 1 cut(s) 2698
AgeI ACCGGT 1 cut(s) 4301
AhdI GACNNNNNGTC 2 cut(s) 2417, 3809
AhlI ACTAGT 1 cut(s) 2363
AjuI GAANNNNNNNTTGG 8 cut(s) 2234, 2266, 2852, 2884, 3299, 3312, 3331, 3344
Alw21I GWGCWC 5 cut(s) 65, 2293, 2503, 2530, 4132
Alw26I GTCTC 8 cut(s) 258, 758, 902, 1340, 1713, 2372, 2828, 3719
Alw44I GTGCAC 1 cut(s) 4128
AlwNI CAGNNNCTG 9 cut(s) 290, 836, 2165, 2837, 3476, 3819, 4009, 4451, 5113
Ama87I CYCGRG 2 cut(s) 2369, 3105
ApaLI GTGCAC 1 cut(s) 4128
ApeKI GCWGC 7 cut(s) 14, 1814, 2918, 4784, 5026, 5110, 5113
ApoI RAATTY 6 cut(s) 820, 1299, 1973, 2015, 4778, 4830
ArsI GACNNNNNNTTYG 2 cut(s) 4486, 4518
AsiGI ACCGGT 1 cut(s) 4301
Asp700I GAANNNNTTC 4 cut(s) 322, 1974, 2995, 4537
Asp718I GGTACC 1 cut(s) 3860
AspLEI GCGC 9 cut(s) 14, 365, 2212, 2259, 2724, 3357, 3657, 3826, 4405
AspS9I GGNCC 6 cut(s) 595, 1380, 1960, 2841, 3382, 4689
AsuC2I CCSGG 1 cut(s) 4921
AvaI CYCGRG 2 cut(s) 2369, 3105
AvaII GGWCC 3 cut(s) 595, 1380, 2841
BaeGI GKGCMC 1 cut(s) 4132
BamHI GGATCC 1 cut(s) 1453
BanI GGYRCC 3 cut(s) 1504, 3860, 4732
BbrPI CACGTG 1 cut(s) 1673
BbsI GAAGAC 4 cut(s) 1139, 2451, 2533, 4983
Bbv12I GWGCWC 5 cut(s) 65, 2293, 2503, 2530, 4132
BbvCI CCTCAGC 1 cut(s) 3678
BbvI GCAGC 7 cut(s) 26, 1801, 2905, 4796, 5013, 5100, 5122
BceAI ACGGC 3 cut(s) 962, 977, 2900
BcgI CGANNNNNNTGC 4 cut(s) 1071, 1105, 4095, 4129
BciVI GTATCC 3 cut(s) 2707, 4177, 4712
BclI TGATCA 4 cut(s) 738, 3550, 3922, 4099
BcnI CCSGG 1 cut(s) 4921
BcoDI GTCTC 8 cut(s) 258, 758, 902, 1340, 1713, 2372, 2828, 3719
BcuI ACTAGT 1 cut(s) 2363
BfaI CTAG 7 cut(s) 335, 428, 1197, 1893, 2364, 2816, 3177
BfmI CTRYAG 6 cut(s) 183, 606, 1160, 3042, 3711, 5027
BfoI RGCGCY 1 cut(s) 366
BfrI CTTAAG 1 cut(s) 3332
BfuAI ACCTGC 2 cut(s) 442, 938
BfuI GTATCC 3 cut(s) 2707, 4177, 4712
BglI GCCNNNNNGGC 1 cut(s) 281
BisI GCNGC 8 cut(s) 15, 22, 1815, 2919, 4785, 5027, 5111, 5114
BlsI GCNGC 8 cut(s) 16, 23, 1816, 2920, 4786, 5028, 5112, 5115
BmcAI AGTACT 3 cut(s) 574, 1921, 2362
Bme18I GGWCC 3 cut(s) 595, 1380, 2841
BmeRI GACNNNNNGTC 2 cut(s) 2417, 3809
BmeT110I CYCGRG 2 cut(s) 2369, 3105
BmgT120I GGNCC 6 cut(s) 595, 1380, 1960, 2841, 3382, 4689
BmrI ACTGGG 3 cut(s) 1920, 2847, 3027
BmuI ACTGGG 3 cut(s) 1920, 2847, 3027
BoxI GACNNNNGTC 1 cut(s) 4350
BpiI GAAGAC 4 cut(s) 1139, 2451, 2533, 4983
BplI GAGNNNNNCTC 6 cut(s) 1320, 1352, 4619, 4651, 4834, 4866
BpmI CTGGAG 6 cut(s) 1058, 3308, 4566, 4738, 5061, 5096
Bpu10I CCTNAGC 2 cut(s) 366, 3678
BpuEI CTTGAG 4 cut(s) 1172, 1322, 1520, 1536
BpuMI CCSGG 1 cut(s) 4921
BsaAI YACGTR 1 cut(s) 1673
BsaBI GATNNNNATC 2 cut(s) 743, 4374
BsaI GGTCTC 2 cut(s) 758, 1713
BsaJI CCNNGG 4 cut(s) 276, 1383, 2075, 2908
BsaWI WCCGGW 4 cut(s) 511, 772, 1377, 4301
Bsc4I CCNNNNNNNGG 5 cut(s) 650, 1384, 1847, 3769, 5074
Bse118I RCCGGY 3 cut(s) 17, 1755, 4301
Bse3DI GCAATG 4 cut(s) 459, 729, 915, 2801
Bse8I GATNNNNATC 2 cut(s) 743, 4374
BseDI CCNNGG 4 cut(s) 276, 1383, 2075, 2908
BseJI GATNNNNATC 2 cut(s) 743, 4374
BseLI CCNNNNNNNGG 5 cut(s) 650, 1384, 1847, 3769, 5074
BseMI GCAATG 4 cut(s) 459, 729, 915, 2801
BseRI GAGGAG 3 cut(s) 293, 2645, 4640
BseSI GKGCMC 1 cut(s) 4132
BseXI GCAGC 7 cut(s) 26, 1801, 2905, 4796, 5013, 5100, 5122
BseYI CCCAGC 3 cut(s) 344, 649, 3215
BsgI GTGCAG 3 cut(s) 668, 2151, 3045
Bsh1236I CGCG 1 cut(s) 4772
BshNI GGYRCC 3 cut(s) 1504, 3860, 4732
BshTI ACCGGT 1 cut(s) 4301
BsiHKAI GWGCWC 5 cut(s) 65, 2293, 2503, 2530, 4132
BsiHKCI CYCGRG 2 cut(s) 2369, 3105
BsiSI CCGG 7 cut(s) 18, 512, 773, 1378, 1756, 4302, 4920
BsiWI CGTACG 1 cut(s) 465
BslFI GGGAC 5 cut(s) 1255, 1366, 1570, 2827, 4358
BslI CCNNNNNNNGG 5 cut(s) 650, 1384, 1847, 3769, 5074
BsmAI GTCTC 8 cut(s) 258, 758, 902, 1340, 1713, 2372, 2828, 3719
BsmFI GGGAC 5 cut(s) 1255, 1366, 1570, 2827, 4358
BsmI GAATGC 3 cut(s) 2269, 2866, 3990
Bso31I GGTCTC 2 cut(s) 758, 1713
BsoBI CYCGRG 2 cut(s) 2369, 3105
Bsp1286I GDGCHC 5 cut(s) 65, 2293, 2503, 2530, 4132
Bsp1407I TGTACA 1 cut(s) 4215
BspFNI CGCG 1 cut(s) 4772
BspHI TCATGA 4 cut(s) 1000, 1966, 3226, 3459
BspMAI CTGCAG 3 cut(s) 610, 1164, 5031
BspMI ACCTGC 2 cut(s) 442, 938
BspQI GCTCTTC 1 cut(s) 2445
BspT107I GGYRCC 3 cut(s) 1504, 3860, 4732
BspTI CTTAAG 1 cut(s) 3332
BspTNI GGTCTC 2 cut(s) 758, 1713
BsrBI CCGCTC 2 cut(s) 161, 1522
BsrDI GCAATG 4 cut(s) 459, 729, 915, 2801
BsrFI RCCGGY 3 cut(s) 17, 1755, 4301
BsrGI TGTACA 1 cut(s) 4215
BssAI RCCGGY 3 cut(s) 17, 1755, 4301
BssECI CCNNGG 4 cut(s) 276, 1383, 2075, 2908
BssNAI GTATAC 1 cut(s) 3161
Bst1107I GTATAC 1 cut(s) 3161
Bst6I CTCTTC 6 cut(s) 1626, 2153, 2331, 2397, 2445, 4947
BstAFI CTTAAG 1 cut(s) 3332
BstAPI GCANNNNNTGC 2 cut(s) 953, 2165
BstAUI TGTACA 1 cut(s) 4215
BstBAI YACGTR 1 cut(s) 1673
BstFNI CGCG 1 cut(s) 4772
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 9 cut(s) 14, 365, 2212, 2259, 2724, 3357, 3657, 3826, 4405
BstMAI GTCTC 8 cut(s) 258, 758, 902, 1340, 1713, 2372, 2828, 3719
BstNSI RCATGY 4 cut(s) 1997, 2702, 2768, 4825
BstPAI GACNNNNGTC 1 cut(s) 4350
BstSFI CTRYAG 6 cut(s) 183, 606, 1160, 3042, 3711, 5027
BstSLI GKGCMC 1 cut(s) 4132
BstUI CGCG 1 cut(s) 4772
BstV1I GCAGC 7 cut(s) 26, 1801, 2905, 4796, 5013, 5100, 5122
BstV2I GAAGAC 4 cut(s) 1139, 2451, 2533, 4983
BstX2I RGATCY 6 cut(s) 484, 1453, 1570, 2275, 4421, 4448
BstXI CCANNNNNNTGG 1 cut(s) 2226
BstYI RGATCY 6 cut(s) 484, 1453, 1570, 2275, 4421, 4448
BstZ17I GTATAC 1 cut(s) 3161
BsuI GTATCC 3 cut(s) 2707, 4177, 4712
BtsI GCAGTG 4 cut(s) 270, 615, 3057, 4113
BveI ACCTGC 2 cut(s) 442, 938
CaiI CAGNNNCTG 9 cut(s) 290, 836, 2165, 2837, 3476, 3819, 4009, 4451, 5113
CciI TCATGA 4 cut(s) 1000, 1966, 3226, 3459
CfoI GCGC 9 cut(s) 14, 365, 2212, 2259, 2724, 3357, 3657, 3826, 4405
Cfr10I RCCGGY 3 cut(s) 17, 1755, 4301
Cfr13I GGNCC 6 cut(s) 595, 1380, 1960, 2841, 3382, 4689
CsiI ACCWGGT 3 cut(s) 244, 897, 3691
Csp6I GTAC 9 cut(s) 466, 573, 632, 1920, 2361, 3861, 4216, 4571, 5006
CspAI ACCGGT 1 cut(s) 4301
CspCI CAANNNNNGTGG 2 cut(s) 1759, 1794
CviQI GTAC 9 cut(s) 466, 573, 632, 1920, 2361, 3861, 4216, 4571, 5006
DraI TTTAAA 1 cut(s) 2677
DraIII CACNNNGTG 1 cut(s) 3068
DriI GACNNNNNGTC 2 cut(s) 2417, 3809
Eam1104I CTCTTC 6 cut(s) 1626, 2153, 2331, 2397, 2445, 4947
Eam1105I GACNNNNNGTC 2 cut(s) 2417, 3809
EarI CTCTTC 6 cut(s) 1626, 2153, 2331, 2397, 2445, 4947
EciI GGCGGA 1 cut(s) 386
Eco147I AGGCCT 3 cut(s) 2145, 2575, 4431
Eco31I GGTCTC 2 cut(s) 758, 1713
Eco47I GGWCC 3 cut(s) 595, 1380, 2841
Eco72I CACGTG 1 cut(s) 1673
Eco88I CYCGRG 2 cut(s) 2369, 3105
EcoO109I RGGNCCY 3 cut(s) 595, 2841, 3382
EcoT22I ATGCAT 3 cut(s) 1995, 4387, 4667
FalI AAGNNNNNCTT 4 cut(s) 3940, 3972, 4195, 4227
FaqI GGGAC 5 cut(s) 1255, 1366, 1570, 2827, 4358
FauI CCCGC 1 cut(s) 1245
FauNDI CATATG 3 cut(s) 1240, 2182, 3659
FbaI TGATCA 4 cut(s) 738, 3550, 3922, 4099
FblI GTMKAC 3 cut(s) 1802, 3160, 4072
Fnu4HI GCNGC 8 cut(s) 15, 22, 1815, 2919, 4785, 5027, 5111, 5114
Fsp4HI GCNGC 8 cut(s) 15, 22, 1815, 2919, 4785, 5027, 5111, 5114
FspAI RTGCGCAY 2 cut(s) 2211, 2258
FspBI CTAG 7 cut(s) 335, 428, 1197, 1893, 2364, 2816, 3177
FspI TGCGCA 4 cut(s) 2211, 2258, 3356, 3656
GlaI GCGC 9 cut(s) 13, 364, 2211, 2258, 2723, 3356, 3656, 3825, 4404
GluI GCNGC 8 cut(s) 15, 22, 1815, 2919, 4785, 5027, 5111, 5114
GsaI CCCAGC 3 cut(s) 348, 653, 3219
GsuI CTGGAG 6 cut(s) 1058, 3308, 4566, 4738, 5061, 5096
HaeII RGCGCY 1 cut(s) 366
HapII CCGG 7 cut(s) 18, 512, 773, 1378, 1756, 4302, 4920
HhaI GCGC 9 cut(s) 14, 365, 2212, 2259, 2724, 3357, 3657, 3826, 4405
Hin6I GCGC 9 cut(s) 12, 363, 2210, 2257, 2722, 3355, 3655, 3824, 4403
HinP1I GCGC 9 cut(s) 12, 363, 2210, 2257, 2722, 3355, 3655, 3824, 4403
HincII GTYRAC 7 cut(s) 553, 703, 1471, 3394, 3586, 4330, 4875
HindII GTYRAC 7 cut(s) 553, 703, 1471, 3394, 3586, 4330, 4875
HpaI GTTAAC 1 cut(s) 4875
HpaII CCGG 7 cut(s) 18, 512, 773, 1378, 1756, 4302, 4920
HpyCH4IV ACGT 6 cut(s) 1060, 1127, 1672, 2172, 4187, 4486
HpySE526I ACGT 6 cut(s) 1060, 1127, 1672, 2172, 4187, 4486
HspAI GCGC 9 cut(s) 12, 363, 2210, 2257, 2722, 3355, 3655, 3824, 4403
KflI GGGWCCC 1 cut(s) 2841
KpnI GGTACC 1 cut(s) 3864
KroI GCCGGC 2 cut(s) 17, 1755
KroNI GCCGGC 2 cut(s) 19, 1757
Ksp22I TGATCA 4 cut(s) 738, 3550, 3922, 4099
KspAI GTTAAC 1 cut(s) 4875
LguI GCTCTTC 1 cut(s) 2445
LmnI GCTCC 9 cut(s) 166, 229, 373, 2296, 2525, 2773, 3289, 3429, 4719
Lsp1109I GCAGC 7 cut(s) 26, 1801, 2905, 4796, 5013, 5100, 5122
MabI ACCWGGT 3 cut(s) 244, 897, 3691
MaeI CTAG 7 cut(s) 335, 428, 1197, 1893, 2364, 2816, 3177
MaeII ACGT 6 cut(s) 1060, 1127, 1672, 2172, 4187, 4486
MaeIII GTNAC 9 cut(s) 28, 1123, 1528, 1668, 2620, 2825, 3742, 5047, 5079
MbiI CCGCTC 2 cut(s) 161, 1522
MflI RGATCY 6 cut(s) 484, 1453, 1570, 2275, 4421, 4448
MhlI GDGCHC 5 cut(s) 65, 2293, 2503, 2530, 4132
MlyI GAGTC 7 cut(s) 53, 703, 1342, 1745, 2315, 2822, 3229
MmeI TCCRAC 8 cut(s) 775, 1152, 2092, 2318, 2572, 3541, 4132, 4507
Mph1103I ATGCAT 3 cut(s) 1995, 4387, 4667
MroNI GCCGGC 2 cut(s) 17, 1755
MroXI GAANNNNTTC 4 cut(s) 322, 1974, 2995, 4537
MslI CAYNNNNRTG 4 cut(s) 1005, 1478, 1998, 3228
MspA1I CMGCKG 3 cut(s) 3080, 3215, 5113
MspCI CTTAAG 1 cut(s) 3332
MspI CCGG 7 cut(s) 18, 512, 773, 1378, 1756, 4302, 4920
Mva1269I GAATGC 3 cut(s) 2269, 2866, 3990
MvnI CGCG 1 cut(s) 4772
NaeI GCCGGC 2 cut(s) 19, 1757
NciI CCSGG 1 cut(s) 4921
NdeI CATATG 3 cut(s) 1240, 2182, 3659
NgoMIV GCCGGC 2 cut(s) 17, 1755
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 5 cut(s) 28, 1668, 2825, 3742, 5047
NsbI TGCGCA 4 cut(s) 2211, 2258, 3356, 3656
NsiI ATGCAT 3 cut(s) 1995, 4387, 4667
NspI RCATGY 4 cut(s) 1997, 2702, 2768, 4825
PaeI GCATGC 3 cut(s) 1997, 2768, 4825
PaeR7I CTCGAG 1 cut(s) 2369
PagI TCATGA 4 cut(s) 1000, 1966, 3226, 3459
PaqCI CACCTGC 1 cut(s) 938
PceI AGGCCT 3 cut(s) 2145, 2575, 4431
PciI ACATGT 1 cut(s) 2698
PciSI GCTCTTC 1 cut(s) 2445
PcsI WCGNNNNNNNCGW 1 cut(s) 1133
PctI GAATGC 3 cut(s) 2269, 2866, 3990
PdiI GCCGGC 2 cut(s) 19, 1757
PdmI GAANNNNTTC 4 cut(s) 322, 1974, 2995, 4537
Pfl23II CGTACG 1 cut(s) 465
PflFI GACNNNGTC 3 cut(s) 2455, 2839, 3581
PfoI TCCNGGA 1 cut(s) 981
PinAI ACCGGT 1 cut(s) 4301
PkrI GCNGC 8 cut(s) 16, 23, 1816, 2920, 4786, 5028, 5112, 5115
PleI GAGTC 7 cut(s) 52, 702, 1341, 1744, 2315, 2822, 3228
PmaCI CACGTG 1 cut(s) 1673
PmlI CACGTG 1 cut(s) 1673
PpsI GAGTC 7 cut(s) 52, 702, 1341, 1744, 2315, 2822, 3228
Ppu21I YACGTR 1 cut(s) 1673
PpuMI RGGWCCY 2 cut(s) 595, 2841
PscI ACATGT 1 cut(s) 2698
PshAI GACNNNNGTC 1 cut(s) 4350
PsiI TTATAA 1 cut(s) 4035
Psp5II RGGWCCY 2 cut(s) 595, 2841
PspCI CACGTG 1 cut(s) 1673
PspFI CCCAGC 3 cut(s) 344, 649, 3215
PspLI CGTACG 1 cut(s) 465
PspPI GGNCC 6 cut(s) 595, 1380, 1960, 2841, 3382, 4689
PspPPI RGGWCCY 2 cut(s) 595, 2841
PstI CTGCAG 3 cut(s) 610, 1164, 5031
PstNI CAGNNNCTG 9 cut(s) 290, 836, 2165, 2837, 3476, 3819, 4009, 4451, 5113
PsuI RGATCY 6 cut(s) 484, 1453, 1570, 2275, 4421, 4448
PsyI GACNNNGTC 3 cut(s) 2455, 2839, 3581
PvuII CAGCTG 2 cut(s) 3215, 5113
RsaI GTAC 9 cut(s) 467, 574, 633, 1921, 2362, 3862, 4217, 4572, 5007
RsaNI GTAC 9 cut(s) 466, 573, 632, 1920, 2361, 3861, 4216, 4571, 5006
RseI CAYNNNNRTG 4 cut(s) 1005, 1478, 1998, 3228
SapI GCTCTTC 1 cut(s) 2445
SatI GCNGC 8 cut(s) 15, 22, 1815, 2919, 4785, 5027, 5111, 5114
Sau96I GGNCC 6 cut(s) 595, 1380, 1960, 2841, 3382, 4689
ScaI AGTACT 3 cut(s) 574, 1921, 2362
SchI GAGTC 7 cut(s) 53, 703, 1342, 1745, 2315, 2822, 3229
SduI GDGCHC 5 cut(s) 65, 2293, 2503, 2530, 4132
SexAI ACCWGGT 3 cut(s) 244, 897, 3691
SfcI CTRYAG 6 cut(s) 183, 606, 1160, 3042, 3711, 5027
Sfr274I CTCGAG 1 cut(s) 2369
SinI GGWCC 3 cut(s) 595, 1380, 2841
SlaI CTCGAG 1 cut(s) 2369
SmiMI CAYNNNNRTG 4 cut(s) 1005, 1478, 1998, 3228
SmlI CTYRAG 6 cut(s) 1151, 1337, 1499, 1551, 2369, 3332
SmoI CTYRAG 6 cut(s) 1151, 1337, 1499, 1551, 2369, 3332
SpeI ACTAGT 1 cut(s) 2363
SphI GCATGC 3 cut(s) 1997, 2768, 4825
SseBI AGGCCT 3 cut(s) 2145, 2575, 4431
SspI AATATT 2 cut(s) 676, 1955
SspMI CTAG 7 cut(s) 335, 428, 1197, 1893, 2364, 2816, 3177
StuI AGGCCT 3 cut(s) 2145, 2575, 4431
TaiI ACGT 6 cut(s) 1063, 1130, 1675, 2175, 4190, 4489
TaqI TCGA 7 cut(s) 2370, 2648, 3364, 3525, 3953, 4392, 4605
TaqII GACCGA 3 cut(s) 782, 1695, 3761
TatI WGTACW 5 cut(s) 572, 631, 1919, 2360, 4215
TauI GCSGC 1 cut(s) 24
TseFI GTSAC 5 cut(s) 28, 1668, 2825, 3742, 5047
TseI GCWGC 7 cut(s) 14, 1814, 2918, 4784, 5026, 5110, 5113
Tsp45I GTSAC 5 cut(s) 28, 1668, 2825, 3742, 5047
TspGWI ACGGA 2 cut(s) 1210, 3968
Tth111I GACNNNGTC 3 cut(s) 2455, 2839, 3581
Vha464I CTTAAG 1 cut(s) 3332
VneI GTGCAC 1 cut(s) 4128
VpaK11BI GGWCC 3 cut(s) 595, 1380, 2841
XapI RAATTY 6 cut(s) 820, 1299, 1973, 2015, 4778, 4830
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 4 cut(s) 1997, 2702, 2768, 4825
XhoI CTCGAG 1 cut(s) 2369
XmiI GTMKAC 3 cut(s) 1802, 3160, 4072
XmnI GAANNNNTTC 4 cut(s) 322, 1974, 2995, 4537
XspI CTAG 7 cut(s) 335, 428, 1197, 1893, 2364, 2816, 3177
ZrmI AGTACT 3 cut(s) 574, 1921, 2362
Zsp2I ATGCAT 3 cut(s) 1995, 4387, 4667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.