MD15G1225500.v1.1

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007281
Physical Location & Seq
Reverse (-)
18314737 .. 18327449
12713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1225500.v1.1.491

Sequence Viewer

Length: 5256 bp
ATGGCGGGCGGCGCAGCTGGCGGTTTTGTGACGAGAGCATTCGAGTCTATGCTCAAGGAGTGCTCTCCTAAAAAGCATGCAGATCTTCAGAAGGCTATTCAGGCTTATTTAGATAGTACGAAAGAGGTCAACCAGACTCAACAAACCGAACCAAGTGAGAAGAATCAAGCTACAACTTCAGCTGGCAATGGAAGTTCTCCTGAAACAGAAGATGGAGCTGCAAAAACTGATACAGAGCCAGGTCAGTCTCAAACTGAGGAGGCAGAGTCCGTTGCCAAGCCAGTGATCACAACCACAACTATTTCAACTGTCCTAGCAAAGGCCGGGAACACCTTAGAAGGAGCTCAGGCAGAGCTTGTTTTGAATCCCCTTCGACTTGCATTTGAGACAAAGAACTTGAAAGTCCTAGAACCTGCTCTGGATTGCATTCATAAACTCATTGCATATGATCATTTAGAGGGAGATCCTGGTCTAGATGATGGTAAAAGTGTACCACTGTTCACAGACCTTCTGAACATGGTTTGCAGTTGCGTTGACAACTCATCACCTGATAGTACCGTTCTGCAAGTGTTGAAGGTTCTTCTTACTGCCGTGGCATCCACGAAGTTCAGAGTACACGGGGAACCTTTGCTGGGACTTATCAGAGTGTGCTACAATATTGCTCTGCACAGCAAGAGTTCCATAAACCAAGCAACGTCAAAGGCAATGCTTACGCAGATGATCAGCATCATATTCAGGAGAATGGAGACTGATCCGGGACTTGAGGTTGCATCAACTGGTTCTGTTGGACATATAGAAGCCATTTCAGGACAAAATTCAAATACAGAAGCCGAAGAAACTTCCTCAGAAGGACAAAGTGAGAAAGAAATGACTTTAGGAGATCAACCTAACCAGGTCAAAGATACACCCATTGCATCTGTTGAGGAACTTCATAATCTAGCTGGCGGTGCTGACATCAAGGGCTTAGAGGCTGTCCTGGACAAAGCTGTGCATCATGAGGATGGTAAAAAGATAACCAGAGGGATTGACCTCGAGAGCATGACCATTGTTCAACATGATGCTTTACTGGTTTTCCGCACTCTTTGCAAGATGGGGATGAAGGAAGACAACAATGAAGTTACTTTGAAGACGAGGATTTTGTCTCTTGAGCTCTTGCAGGGTTTATTGGAAGGGGTTGGCCATCCATTTACTAGGAACTTCCATTTTATTGACTCGGTGAAAGCATACCTATCATATGCTTTGTTGCGGGCTTCAGTTTCACAGTCTCCTGTCATATTTCAGTATGCAACTGGAATTTTTTTAGTGCTTTTGTTGCGCTTCAGAGAAAGCCTCAAGGGTGAAATAGGCATCTTCTTTCCCTTGATAGTTCTACGATCACTGGATGGCTTGGATTTTCCAATTAACCAGAAATTAAGCGTTCTTAGGATGGTTGAAAAAGTTTGCAAGGATCCTCAGATGCTTGTTGACATATTTGTGAACTATGATTGTGATCTTGAGGCACCAAACCTGTTTGAGCGTATGGTTACAACTCTATCCAGAATTTCTCAAGGGACTCAAAACGCAGATCCAAATGTGGTTGCTGTATCACCGGCAACCTCAATTAAAGGTTCATCTCTTCAGTGTCTCGTGAATGTGCTTAAATCACTAGTTGATTGGGAAAAGTCGCGTGGAGAATCAGAAAACCAGAGTAATAATACTCGGTCTCTAGATGGTGAAGCTAAAGAGTCTGTTGATGTAACCAGTAACTTTGAGAAGGCTAAAGCTCATAAATCTACATTGGAAGCTGCCATCTCTGAGTTCAATAGGCAACCAGTAAAGGGTGTAGAGTATCTGAAATCAAATAAATTGGTGGAAAATACACCCAGTTCAGTTGCCCAATTTTTGAGAAGTACTCCCTGTCTGGACAAGGCTATGATTGGGGAATATTTGGGTCACCATGAAGAATTTCCCCTTGCTGTAATGCATGCTTATGTAGATTCCATGAAGTTTTCAGGAATGAAGTTTGACACTGCAATTCGTGAACTTCTTAAAGGATTCAGACTTCCAGGGGAAGCTCAAAAGATAGATCGCATCATGGAGAAATTTGCAGAAAGATATTGTGCAGATAATCCAGGTCTTTTCAAAAATGCAGACACTGCATACATTTTAGCATATGCGGTTATATTGTTGAATACTGATGCGCATAATCCAATGGTGTGGCCAAAAATGTCCAAGTTGGATTTTATACGCATGAATGCTGTGGATGATGCAGAAGAGTGTGCCCCAACTGAACTTCTGGAAGAAATCTATGATTCAATTGTTAAAGAAGAGATAAGGATGAAAGATGAGACTGCTAGTCTTGAGAAAAGTGGAAAATATAAGCCAGAAGGTGAAGAGAGAGGCCGCCTTGTCAGTATCCTTAATCTGGCTCTTCCCACAAGTGCGCTGTCCGTTGATACTAAGTCTGAAAGTGAAGCCATCATTAAGAAAACACAAGCTATATTTCGGAACCAAGGAGCAAAAAGAGGGGTCTTCTATACTACACAGCAGTTAGAGCTAGTAAGGCCTATGGTTGAAGCTGTAGGTTGGCCTTTGCTCGCTACTTTTTCTGTTACAATGGAGGAGGGTGAGAATAAGTCGAGGGTTGTTCTCTGTATGGAGGGATTTAAAGCTGGAATACACATTACACATGTTCTCGGAATGAATACCATGCGATATGCCTTTTTAACATCTCTGGTCAGATTTACTTTCTTGCATGCTCCGAAGGAAATGCGTAGTAAAAATGTGGAAGCACTGCGAACTCTACTATCTCTTTGTGACTCAGGGACTGGCTCCCTTCGAGATACATGGAATGCAGTTTTAGAATGTGTTTCTCGGCTTGAGTTCATTACTTCAAATCCCTCTATAGCAGCAACAGTCATGCAAGGATCAAACCAAATCTCCAAAGACGCTCTTCTTCAATCTTTAAGGGAGTTGGCTGGAAAACCTTCTGAACAAGTATTTGTGAATAGCGTTCAGCTTCCTAGTGATTCTGTAGTGGAGTTCTTCACTGCACTCTGTGGTGTATCAGCTGAAGAACTAAAACAAACTCCAGCTCGTGTGTTTAGCTTGCAAAAACTTGTTGAGATCAGCTACTACAACATGGCTCGTATACGCATGGTCTGGGCTAGAATATGGTCTGTTTTGGCAAACCACTTTATTTCAGCTGGGAGTCAGCATGATGAAAAAATTGCTATGTATGCAATAGATTCCCTGAGGCAGCTTGGTATGAAGTATCTGGAGCGGGCGGAACTTGCCAATTTCACATTCCAAAATGACATTCTTAAACCTTTTGTTGTTCTTATGCGCAATAGTCAAAGTGAAACCATAAGGAGCTTAATAGTTGACTGCATCGTTCAAATGATAAAATCCAAAGTAGGGAGCATCAAGTCTGGATGGCGTAACGTTTTCATGATTTTTACAGCTGCTGCAGATGATGAATTGGAATCAATTGTTGAGAGTGCATTTGAAAATGTAGAACAGGTTATCTTGGAACACTTCGATCAAGTAGTTGGAGATTGTTTCATGGACTGTGTCAACTGTCTTATCAGGTTTGCTAATAATAGAACTTCACACCGTATAAGTTTGAAGGCTATTGCACTATTGCGCATATGCGAGGATCGTCTGGCAGAGGGTCTTATACCTGGTGGTGCTTTGAGGCCTATTGACGTAAATGTGGATACAACTTTTGATGTGACTGAACATTACTGGTTCCCAATGCTGGCTGGTCTGTCTGATCTGACATCAGATCCAAGACCAGAGGTCAGAAGCTGTGCGCTTGAAGTTTTGTTTGATCTACTGAATGAGAGAGGTAGCAAATTCTCATCAGCATTTTGGGAGAGCATTTTTCATCGGGTCTTGTTTCCCATATTTGATCACGTGAGACATGTTGGAAAGGAAAGCTCGGTTTCCTCTGACGATGTATGGTTCCGTGAAACAAGCATTCACTCGCTGCAGTTGCTTTGCAACCTTTTCAACACGTTTTATAAGGAAGTATGTTTCATGTTGCCACCCCTACTCAGTCTACTTTTGGATTGTGCCAAAAAGACTGATCAAGCAGTTGTTTCATTATCTCTGGGTGCACTTGTGCATCTCATTGAAGTTGGAGGACATCAATTTAGTGAGAGCGACTGGGATACATTGTTGAAAAGCCTAAGAGATGCTTTGTACACAACACAACCACTTGAACTGCTAAACGCTTTGGGATTTGAGAATCTGAAGAACAATAATAGGGTGCTAACCGGAGATTTAGGGGTCAATTCAGGTGATAGCCCTTCCATAAAGTCTGATTATGAGGGAGTGGATAGCCGTCATTTTGAAGTCGATAATGGAAGAAATCCAGTATTGGTTAAACAAAACTCGGGGGTGCAAATGAACACGGATGGATCTGAAGGGCTTCCATCACCATCAGGAAGTGCTTCAAAATCTGCTGAAGGTCGAAGCCTCCAACGGAGTCAAACAATAGGTCAAAGGATTATGGACAATCTCTTTCTTAGAAATCTTACTTCTAAGCCTAAGGCTATTCCATCGGAGGCTTCAGTACCATCTTCTCCGATTAGGGTGCCTGAGGCTGTCGAACCTGATATCAGAGATAAGGAGGAAAGTTCACTGTTGGGAACTTGTCGGGGCAAATGCATCACTCAACTTTTACTTCTTGGTGCCATTGATAGCATTCAGAAGAAATACTGGAGCAACTTGAAAACACCACAAAAGATTGCTATTATGGATATTTTGCTGTCTGCATTAGAGTTCGCAGCTTCGTACAATTCATATACCAATCTCAGAACACGCATGCACCAAGTTCATGATGAGAGGCCACCTCTGAATCTTCTTCGCCAAGAATTAGCAGGAACTTGTATATATCTGGAGATCTTACAGAAAGCAACCTCCGGGTTTAGTGCTGACAAAGAGGGAGAAACTAATGGTGAAGAGAAAGTCGAAGGATTAGCCGAAGAGAAACTGGTGTCTTTCTGTGAACAGGTACTCAGGGAGGCATCTGATTTGCAGTCCAGTCCTGGGGAGACTACTAATATGGATATTCATCGGGTTCTGGAATTACGATCTCCAATAATTATCAAGGTGATTAAGGGCATGTGCTTTATGAACCAACAGATTTTCAGACGGCATCTTAGAGACTTCTATCCGTTGCTTACAAAACTCGTATGCTGTGACCAGATGGATGTTCGCGGAGCTCTTGGTGATCTCTTCAGGGCACAATTGAAAGCACTTCTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1752

Amino Acids

194.11

Weight (kDa)

5.46

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 110 - 251 5.7e-26 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 351 - 503 8.2e-40 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 590 - 771 8e-70 Sec7 domain
Sec7-like_HDS PF09324 1084 - 1166 2.2e-27 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1240 - 1305 2.5e-07 C-terminal region of Mon2 protein
BIG2_C PF20252 1553 - 1742 1.1e-26 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4005
Acc16I TGCGCA 3 cut(s) 2181, 3326, 3626
Acc36I ACCTGC 1 cut(s) 421
AccB1I GGYRCC 3 cut(s) 1498, 4579, 4676
AccBSI CCGCTC 1 cut(s) 3262
AccI GTMKAC 2 cut(s) 3130, 4042
AccII CGCG 2 cut(s) 1666, 5205
AclI AACGTT 1 cut(s) 3423
AclWI GGATC 9 cut(s) 458, 746, 1442, 1455, 1559, 2914, 3645, 3761, 4411
AcoI YGGCCR 2 cut(s) 1177, 2198
AcsI RAATTY 6 cut(s) 814, 1293, 1539, 1943, 2081, 3836
AcvI CACGTG 1 cut(s) 3898
AdeI CACNNNGTG 1 cut(s) 3038
AfaI GTAC 9 cut(s) 118, 492, 556, 615, 1891, 4187, 4560, 4781, 5001
AfiI CCNNNNNNNGG 7 cut(s) 319, 632, 1817, 2404, 3396, 3739, 5034
AflIII ACRYGT 3 cut(s) 2668, 3904, 3996
AhdI GACNNNNNGTC 2 cut(s) 2334, 3779
AhlI ACTAGT 1 cut(s) 1645
AjnI CCWGG 8 cut(s) 238, 466, 891, 975, 2044, 2110, 3661, 5032
AjuI GAANNNNNNNTTGG 4 cut(s) 3269, 3282, 3301, 3314
Alw21I GWGCWC 5 cut(s) 65, 346, 1152, 4102, 5212
Alw44I GTGCAC 1 cut(s) 4098
AlwI GGATC 9 cut(s) 458, 746, 1442, 1455, 1559, 2914, 3645, 3761, 4411
AlwNI CAGNNNCTG 4 cut(s) 2135, 2807, 3446, 3789
Ama87I CYCGRG 2 cut(s) 1031, 4378
AoxI GGCC 8 cut(s) 321, 1177, 2198, 2380, 2543, 2567, 3677, 4832
ApaLI GTGCAC 1 cut(s) 4098
ApeKI GCWGC 9 cut(s) 14, 218, 1784, 2888, 3238, 3443, 3446, 3970, 4772
ApoI RAATTY 6 cut(s) 814, 1293, 1539, 1943, 2081, 3836
ArsI GACNNNNNNTTYG 4 cut(s) 1120, 1152, 4468, 4500
Asp700I GAANNNNTTC 4 cut(s) 1944, 2965, 4413, 4435
AspLEI GCGC 7 cut(s) 14, 1317, 2182, 2425, 3327, 3627, 3796
AsuC2I CCSGG 3 cut(s) 325, 756, 4909
AvaI CYCGRG 2 cut(s) 1031, 4378
AxyI CCTNAGG 3 cut(s) 3233, 4533, 4584
BaeGI GKGCMC 3 cut(s) 2263, 4102, 5233
BalI TGGCCA 2 cut(s) 1179, 2200
BamHI GGATCC 1 cut(s) 1447
BanI GGYRCC 3 cut(s) 1498, 4579, 4676
BanII GRGCYC 3 cut(s) 346, 1152, 5212
BauI CACGAG 2 cut(s) 1625, 3075
BbrPI CACGTG 1 cut(s) 3898
BbsI GAAGAC 3 cut(s) 1110, 1133, 2503
Bbv12I GWGCWC 5 cut(s) 65, 346, 1152, 4102, 5212
BbvI GCAGC 9 cut(s) 26, 205, 1771, 2900, 3250, 3430, 3433, 3957, 4784
BceAI ACGGC 3 cut(s) 575, 4311, 5156
BcgI CGANNNNNNTGC 4 cut(s) 2731, 2765, 4561, 4595
BciT130I CCWGG 8 cut(s) 240, 468, 893, 977, 2046, 2112, 3663, 5034
BciVI GTATCC 3 cut(s) 2405, 3691, 4147
BclI TGATCA 5 cut(s) 285, 448, 720, 3892, 4069
BcnI CCSGG 3 cut(s) 325, 756, 4909
BcuI ACTAGT 1 cut(s) 1645
BfmI CTRYAG 5 cut(s) 2559, 2883, 3012, 3447, 3971
BfuAI ACCTGC 1 cut(s) 421
BfuI GTATCC 3 cut(s) 2405, 3691, 4147
BglII AGATCT 2 cut(s) 82, 4887
BmcAI AGTACT 1 cut(s) 1891
BmeRI GACNNNNNGTC 2 cut(s) 2334, 3779
BmeT110I CYCGRG 2 cut(s) 1031, 4378
BmiI GGNNCC 9 cut(s) 624, 1449, 1500, 2489, 2812, 3731, 3947, 4581, 4678
BmrI ACTGGG 2 cut(s) 1857, 4159
BmuI ACTGGG 2 cut(s) 1857, 4159
BpiI GAAGAC 3 cut(s) 1110, 1133, 2503
BplI GAGNNNNNCTC 6 cut(s) 1314, 1346, 1876, 1908, 4822, 4854
BpmI CTGGAG 4 cut(s) 3054, 3278, 4726, 4904
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 8 cut(s) 38, 782, 1166, 1316, 1514, 1530, 2360, 2879
BpuMI CCSGG 3 cut(s) 325, 756, 4909
BsaAI YACGTR 1 cut(s) 3898
BsaBI GATNNNNATC 3 cut(s) 725, 1488, 5058
BsaI GGTCTC 1 cut(s) 1707
BsaJI CCNNGG 4 cut(s) 591, 2045, 2491, 5033
BsaWI WCCGGW 1 cut(s) 4259
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 2903, 2933
Bsc4I CCNNNNNNNGG 7 cut(s) 319, 632, 1817, 2404, 3396, 3739, 5034
Bse118I RCCGGY 1 cut(s) 1588
Bse21I CCTNAGG 3 cut(s) 3233, 4533, 4584
Bse3DI GCAATG 4 cut(s) 193, 438, 711, 909
Bse8I GATNNNNATC 3 cut(s) 725, 1488, 5058
BseBI CCWGG 8 cut(s) 240, 468, 893, 977, 2046, 2112, 3663, 5034
BseDI CCNNGG 4 cut(s) 591, 2045, 2491, 5033
BseJI GATNNNNATC 3 cut(s) 725, 1488, 5058
BseLI CCNNNNNNNGG 7 cut(s) 319, 632, 1817, 2404, 3396, 3739, 5034
BseMI GCAATG 4 cut(s) 193, 438, 711, 909
BseRI GAGGAG 2 cut(s) 272, 2615
BseSI GKGCMC 3 cut(s) 2263, 4102, 5233
BseXI GCAGC 9 cut(s) 26, 205, 1771, 2900, 3250, 3430, 3433, 3957, 4784
BseYI CCCAGC 2 cut(s) 631, 3185
BsgI GTGCAG 3 cut(s) 650, 2121, 3015
Bsh1236I CGCG 2 cut(s) 1666, 5205
BshFI GGCC 8 cut(s) 323, 1179, 2200, 2382, 2545, 2569, 3679, 4834
BshNI GGYRCC 3 cut(s) 1498, 4579, 4676
BsiHKAI GWGCWC 5 cut(s) 65, 346, 1152, 4102, 5212
BsiHKCI CYCGRG 2 cut(s) 1031, 4378
BsiSI CCGG 5 cut(s) 324, 755, 1589, 4260, 4908
BslFI GGGAC 4 cut(s) 648, 771, 1564, 2818
BslI CCNNNNNNNGG 7 cut(s) 319, 632, 1817, 2404, 3396, 3739, 5034
BsmFI GGGAC 4 cut(s) 648, 771, 1564, 2818
BsmI GAATGC 6 cut(s) 38, 426, 2239, 2836, 3960, 4689
BsnI GGCC 8 cut(s) 323, 1179, 2200, 2382, 2545, 2569, 3679, 4834
Bso31I GGTCTC 1 cut(s) 1707
BsoBI CYCGRG 2 cut(s) 1031, 4378
Bsp1286I GDGCHC 7 cut(s) 65, 346, 1152, 2263, 4102, 5212, 5233
Bsp1407I TGTACA 1 cut(s) 4185
BspANI GGCC 8 cut(s) 323, 1179, 2200, 2382, 2545, 2569, 3679, 4834
BspFNI CGCG 2 cut(s) 1666, 5205
BspHI TCATGA 3 cut(s) 994, 3429, 4822
BspLI GGNNCC 9 cut(s) 624, 1449, 1500, 2489, 2812, 3731, 3947, 4581, 4678
BspMAI CTGCAG 2 cut(s) 3451, 3975
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 9 cut(s) 458, 746, 1442, 1455, 1559, 2914, 3645, 3761, 4411
BspQI GCTCTTC 2 cut(s) 2415, 2937
BspT107I GGYRCC 3 cut(s) 1498, 4579, 4676
BspTNI GGTCTC 1 cut(s) 1707
BsrBI CCGCTC 1 cut(s) 3262
BsrDI GCAATG 4 cut(s) 193, 438, 711, 909
BsrFI RCCGGY 1 cut(s) 1588
BsrGI TGTACA 1 cut(s) 4185
BssAI RCCGGY 1 cut(s) 1588
BssECI CCNNGG 4 cut(s) 591, 2045, 2491, 5033
BssNAI GTATAC 1 cut(s) 3131
BssSI CACGAG 2 cut(s) 1625, 3075
BssT1I CCWWGG 1 cut(s) 2491
Bst1107I GTATAC 1 cut(s) 3131
Bst2BI CACGAG 2 cut(s) 1625, 3075
Bst2UI CCWGG 8 cut(s) 240, 468, 893, 977, 2046, 2112, 3663, 5034
Bst4CI ACNGT 9 cut(s) 310, 498, 559, 1263, 2896, 3551, 3560, 3596, 4629
Bst6I CTCTTC 9 cut(s) 1620, 2247, 2301, 2367, 2415, 2937, 4941, 4965, 5228
BstAPI GCANNNNNTGC 2 cut(s) 1083, 2135
BstAUI TGTACA 1 cut(s) 4185
BstBAI YACGTR 1 cut(s) 3898
BstDSI CCRYGG 1 cut(s) 591
BstEII GGTNACC 1 cut(s) 1931
BstENI CCTNNNNNAGG 1 cut(s) 317
BstFNI CGCG 2 cut(s) 1666, 5205
BstHHI GCGC 7 cut(s) 14, 1317, 2182, 2425, 3327, 3627, 3796
BstNI CCWGG 8 cut(s) 240, 468, 893, 977, 2046, 2112, 3663, 5034
BstNSI RCATGY 7 cut(s) 80, 1967, 2672, 2738, 3908, 4813, 5113
BstPI GGTNACC 1 cut(s) 1931
BstSFI CTRYAG 5 cut(s) 2559, 2883, 3012, 3447, 3971
BstSLI GKGCMC 3 cut(s) 2263, 4102, 5233
BstUI CGCG 2 cut(s) 1666, 5205
BstV1I GCAGC 9 cut(s) 26, 205, 1771, 2900, 3250, 3430, 3433, 3957, 4784
BstV2I GAAGAC 3 cut(s) 1110, 1133, 2503
BstX2I RGATCY 7 cut(s) 82, 463, 1447, 1564, 3766, 4403, 4887
BstXI CCANNNNNNTGG 1 cut(s) 2196
BstYI RGATCY 7 cut(s) 82, 463, 1447, 1564, 3766, 4403, 4887
BstZ17I GTATAC 1 cut(s) 3131
Bsu36I CCTNAGG 3 cut(s) 3233, 4533, 4584
BsuI GTATCC 3 cut(s) 2405, 3691, 4147
BsuRI GGCC 8 cut(s) 323, 1179, 2200, 2382, 2545, 2569, 3679, 4834
BtgI CCRYGG 1 cut(s) 591
BtsI GCAGTG 4 cut(s) 2007, 2133, 2771, 3027
BtsIMutI CAGTG 9 cut(s) 288, 494, 1376, 1625, 2007, 2133, 2771, 3027, 4625
BveI ACCTGC 1 cut(s) 421
CaiI CAGNNNCTG 4 cut(s) 2135, 2807, 3446, 3789
CciI TCATGA 3 cut(s) 994, 3429, 4822
CfoI GCGC 7 cut(s) 14, 1317, 2182, 2425, 3327, 3627, 3796
Cfr10I RCCGGY 1 cut(s) 1588
CseI GACGC 1 cut(s) 2936
CsiI ACCWGGT 2 cut(s) 891, 3661
Csp6I GTAC 9 cut(s) 117, 491, 555, 614, 1890, 4186, 4559, 4780, 5000
CviQI GTAC 9 cut(s) 117, 491, 555, 614, 1890, 4186, 4559, 4780, 5000
DraI TTTAAA 1 cut(s) 2647
DraIII CACNNNGTG 1 cut(s) 3038
DriI GACNNNNNGTC 2 cut(s) 2334, 3779
EaeI YGGCCR 2 cut(s) 1177, 2198
Eam1104I CTCTTC 9 cut(s) 1620, 2247, 2301, 2367, 2415, 2937, 4941, 4965, 5228
Eam1105I GACNNNNNGTC 2 cut(s) 2334, 3779
EarI CTCTTC 9 cut(s) 1620, 2247, 2301, 2367, 2415, 2937, 4941, 4965, 5228
EciI GGCGGA 1 cut(s) 3281
Ecl136II GAGCTC 3 cut(s) 344, 1150, 5210
Eco130I CCWWGG 1 cut(s) 2491
Eco147I AGGCCT 2 cut(s) 2545, 3679
Eco24I GRGCYC 3 cut(s) 346, 1152, 5212
Eco31I GGTCTC 1 cut(s) 1707
Eco32I GATATC 1 cut(s) 4603
Eco53kI GAGCTC 3 cut(s) 344, 1150, 5210
Eco72I CACGTG 1 cut(s) 3898
Eco81I CCTNAGG 3 cut(s) 3233, 4533, 4584
Eco88I CYCGRG 2 cut(s) 1031, 4378
Eco91I GGTNACC 1 cut(s) 1931
EcoICRI GAGCTC 3 cut(s) 344, 1150, 5210
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoO65I GGTNACC 1 cut(s) 1931
EcoRII CCWGG 8 cut(s) 238, 466, 891, 975, 2044, 2110, 3661, 5032
EcoRV GATATC 1 cut(s) 4603
EcoT14I CCWWGG 1 cut(s) 2491
EcoT22I ATGCAT 2 cut(s) 1965, 4655
EcoT38I GRGCYC 3 cut(s) 346, 1152, 5212
ErhI CCWWGG 1 cut(s) 2491
FalI AAGNNNNNCTT 4 cut(s) 2916, 2948, 4165, 4197
FaqI GGGAC 4 cut(s) 648, 771, 1564, 2818
FauI CCCGC 2 cut(s) 1239, 3255
FauNDI CATATG 4 cut(s) 445, 1234, 2152, 3629
FbaI TGATCA 5 cut(s) 285, 448, 720, 3892, 4069
FblI GTMKAC 2 cut(s) 3130, 4042
FriOI GRGCYC 3 cut(s) 346, 1152, 5212
FspAI RTGCGCAY 1 cut(s) 2181
FspI TGCGCA 3 cut(s) 2181, 3326, 3626
GlaI GCGC 7 cut(s) 13, 1316, 2181, 2424, 3326, 3626, 3795
GsaI CCCAGC 2 cut(s) 635, 3189
GsuI CTGGAG 4 cut(s) 3054, 3278, 4726, 4904
HaeIII GGCC 8 cut(s) 323, 1179, 2200, 2382, 2545, 2569, 3679, 4834
HapII CCGG 5 cut(s) 324, 755, 1589, 4260, 4908
HgaI GACGC 1 cut(s) 2936
HhaI GCGC 7 cut(s) 14, 1317, 2182, 2425, 3327, 3627, 3796
Hin6I GCGC 7 cut(s) 12, 1315, 2180, 2423, 3325, 3625, 3794
HinP1I GCGC 7 cut(s) 12, 1315, 2180, 2423, 3325, 3625, 3794
HincII GTYRAC 5 cut(s) 130, 535, 1465, 3364, 3556
HindII GTYRAC 5 cut(s) 130, 535, 1465, 3364, 3556
HpaII CCGG 5 cut(s) 324, 755, 1589, 4260, 4908
HpyCH4III ACNGT 9 cut(s) 310, 498, 559, 1263, 2896, 3551, 3560, 3596, 4629
HpyCH4IV ACGT 5 cut(s) 695, 3423, 3687, 3897, 3998
HpySE526I ACGT 5 cut(s) 695, 3423, 3687, 3897, 3998
HspAI GCGC 7 cut(s) 12, 1315, 2180, 2423, 3325, 3625, 3794
Ksp22I TGATCA 5 cut(s) 285, 448, 720, 3892, 4069
LguI GCTCTTC 2 cut(s) 2415, 2937
Lsp1109I GCAGC 9 cut(s) 26, 205, 1771, 2900, 3250, 3430, 3433, 3957, 4784
MabI ACCWGGT 2 cut(s) 891, 3661
MaeII ACGT 5 cut(s) 695, 3423, 3687, 3897, 3998
MbiI CCGCTC 1 cut(s) 3262
MfeI CAATTG 3 cut(s) 2295, 3468, 5234
MflI RGATCY 7 cut(s) 82, 463, 1447, 1564, 3766, 4403, 4887
MhlI GDGCHC 7 cut(s) 65, 346, 1152, 2263, 4102, 5212, 5233
MlsI TGGCCA 2 cut(s) 1179, 2200
MluNI TGGCCA 2 cut(s) 1179, 2200
MlyI GAGTC 9 cut(s) 53, 130, 275, 1205, 1546, 1733, 2792, 3199, 4480
MmeI TCCRAC 6 cut(s) 766, 2196, 3511, 3889, 4102, 4489
Mox20I TGGCCA 2 cut(s) 1179, 2200
Mph1103I ATGCAT 2 cut(s) 1965, 4655
MroXI GAANNNNTTC 4 cut(s) 1944, 2965, 4413, 4435
MscI TGGCCA 2 cut(s) 1179, 2200
MslI CAYNNNNRTG 3 cut(s) 999, 1472, 1968
Msp20I TGGCCA 2 cut(s) 1179, 2200
MspA1I CMGCKG 5 cut(s) 17, 182, 3050, 3185, 3443
MspI CCGG 5 cut(s) 324, 755, 1589, 4260, 4908
MunI CAATTG 3 cut(s) 2295, 3468, 5234
Mva1269I GAATGC 6 cut(s) 38, 426, 2239, 2836, 3960, 4689
MvaI CCWGG 8 cut(s) 240, 468, 893, 977, 2046, 2112, 3663, 5034
MvnI CGCG 2 cut(s) 1666, 5205
NciI CCSGG 3 cut(s) 325, 756, 4909
NdeI CATATG 4 cut(s) 445, 1234, 2152, 3629
NlaIV GGNNCC 9 cut(s) 624, 1449, 1500, 2489, 2812, 3731, 3947, 4581, 4678
NmeAIII GCCGAG 1 cut(s) 2833
NmuCI GTSAC 5 cut(s) 28, 1931, 2795, 3712, 5186
NsbI TGCGCA 3 cut(s) 2181, 3326, 3626
NsiI ATGCAT 2 cut(s) 1965, 4655
NspI RCATGY 7 cut(s) 80, 1967, 2672, 2738, 3908, 4813, 5113
PaeI GCATGC 4 cut(s) 80, 1967, 2738, 4813
PaeR7I CTCGAG 1 cut(s) 1031
PagI TCATGA 3 cut(s) 994, 3429, 4822
PceI AGGCCT 2 cut(s) 2545, 3679
PciI ACATGT 2 cut(s) 2668, 3904
PciSI GCTCTTC 2 cut(s) 2415, 2937
PctI GAATGC 6 cut(s) 38, 426, 2239, 2836, 3960, 4689
PdmI GAANNNNTTC 4 cut(s) 1944, 2965, 4413, 4435
PflFI GACNNNGTC 1 cut(s) 3551
PfoI TCCNGGA 2 cut(s) 754, 975
PleI GAGTC 9 cut(s) 52, 130, 274, 1205, 1546, 1732, 2792, 3198, 4479
PmaCI CACGTG 1 cut(s) 3898
PmlI CACGTG 1 cut(s) 3898
PpsI GAGTC 9 cut(s) 52, 130, 274, 1205, 1546, 1732, 2792, 3198, 4479
Ppu21I YACGTR 1 cut(s) 3898
PscI ACATGT 2 cut(s) 2668, 3904
PsiI TTATAA 1 cut(s) 4005
Psp124BI GAGCTC 3 cut(s) 346, 1152, 5212
Psp1406I AACGTT 1 cut(s) 3423
Psp6I CCWGG 8 cut(s) 238, 466, 891, 975, 2044, 2110, 3661, 5032
PspCI CACGTG 1 cut(s) 3898
PspEI GGTNACC 1 cut(s) 1931
PspFI CCCAGC 2 cut(s) 631, 3185
PspGI CCWGG 8 cut(s) 238, 466, 891, 975, 2044, 2110, 3661, 5032
PspN4I GGNNCC 9 cut(s) 624, 1449, 1500, 2489, 2812, 3731, 3947, 4581, 4678
PstI CTGCAG 2 cut(s) 3451, 3975
PstNI CAGNNNCTG 4 cut(s) 2135, 2807, 3446, 3789
PsuI RGATCY 7 cut(s) 82, 463, 1447, 1564, 3766, 4403, 4887
PsyI GACNNNGTC 1 cut(s) 3551
PvuII CAGCTG 5 cut(s) 17, 182, 3050, 3185, 3443
RsaI GTAC 9 cut(s) 118, 492, 556, 615, 1891, 4187, 4560, 4781, 5001
RsaNI GTAC 9 cut(s) 117, 491, 555, 614, 1890, 4186, 4559, 4780, 5000
RseI CAYNNNNRTG 3 cut(s) 999, 1472, 1968
SacI GAGCTC 3 cut(s) 346, 1152, 5212
SapI GCTCTTC 2 cut(s) 2415, 2937
ScaI AGTACT 1 cut(s) 1891
SchI GAGTC 9 cut(s) 53, 130, 275, 1205, 1546, 1733, 2792, 3199, 4480
SduI GDGCHC 7 cut(s) 65, 346, 1152, 2263, 4102, 5212, 5233
SexAI ACCWGGT 2 cut(s) 891, 3661
SfcI CTRYAG 5 cut(s) 2559, 2883, 3012, 3447, 3971
Sfr274I CTCGAG 1 cut(s) 1031
SlaI CTCGAG 1 cut(s) 1031
SmiMI CAYNNNNRTG 3 cut(s) 999, 1472, 1968
SmlI CTYRAG 9 cut(s) 53, 761, 1031, 1145, 1331, 1493, 1545, 2339, 2858
SmoI CTYRAG 9 cut(s) 53, 761, 1031, 1145, 1331, 1493, 1545, 2339, 2858
SpeI ACTAGT 1 cut(s) 1645
SphI GCATGC 4 cut(s) 80, 1967, 2738, 4813
SseBI AGGCCT 2 cut(s) 2545, 3679
SspI AATATT 2 cut(s) 658, 1925
SstI GAGCTC 3 cut(s) 346, 1152, 5212
StuI AGGCCT 2 cut(s) 2545, 3679
StyI CCWWGG 1 cut(s) 2491
TaaI ACNGT 9 cut(s) 310, 498, 559, 1263, 2896, 3551, 3560, 3596, 4629
TaiI ACGT 5 cut(s) 698, 3426, 3690, 3900, 4001
TaqII GACCGA 1 cut(s) 1689
TatI WGTACW 3 cut(s) 613, 1889, 4185
TauI GCSGC 2 cut(s) 12, 2385
TscAI CASTG 9 cut(s) 288, 501, 1383, 1625, 2014, 2140, 2778, 3034, 4632
TseFI GTSAC 5 cut(s) 28, 1931, 2795, 3712, 5186
TseI GCWGC 9 cut(s) 14, 218, 1784, 2888, 3238, 3443, 3446, 3970, 4772
Tsp45I GTSAC 5 cut(s) 28, 1931, 2795, 3712, 5186
TspGWI ACGGA 6 cut(s) 259, 2419, 3938, 4412, 4483, 5151
TspRI CASTG 9 cut(s) 288, 501, 1383, 1625, 2014, 2140, 2778, 3034, 4632
Tth111I GACNNNGTC 1 cut(s) 3551
VneI GTGCAC 1 cut(s) 4098
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 6 cut(s) 814, 1293, 1539, 1943, 2081, 3836
XbaI TCTAGA 2 cut(s) 472, 1705
XceI RCATGY 7 cut(s) 80, 1967, 2672, 2738, 3908, 4813, 5113
XhoI CTCGAG 1 cut(s) 1031
XmiI GTMKAC 2 cut(s) 3130, 4042
XmnI GAANNNNTTC 4 cut(s) 1944, 2965, 4413, 4435
ZrmI AGTACT 1 cut(s) 1891
Zsp2I ATGCAT 2 cut(s) 1965, 4655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.