MD02G1105600.v1.1

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007281
Physical Location & Seq
Reverse (-)
8554391 .. 8572699
18309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1105600.v1.1.491

Sequence Viewer

Length: 5241 bp
ATGCTCAAGGAGTGCTCTCCTAAGAAGCATGCAGATCTTCAGAAGACTATTCAGGCTTATTTAGATAGTACGAAAGAGGTCAACCAGACTCCACAAACCGAACCAAGTGAGAAGAATCAAGCTACAACTTCAGCTGACGATGGAAGTTCTCCTGAAACAGAAGGTGAAGCTGCAAAAACTGATACAGAACCAGGTCAGTCTCAAACTGAGGAGGCAGAGTCCGTTGCCAAGCCGGCGATCACCACCACAACTATTTCAACTGTCCTAGCAAAGGCCGGGAACACCTTAGAAGGAGCTCAGGCAGAGCTTGTTTTGAATCCCCTACGACTTGCGTTGGAGACAAAGAACTTGAAAGTCCTAGAACCTGCTCTGGATTGCCTTCATAAACTCATTGCATATGATCATTTAGAGGGAGATCCTGGTCTAGAGGATGGTAAAAGTGTACCGCTGTTCTCAGACCTTCTGAACATGGTTTGCAGTTGCGTTGACAACTCATCACCTGATAGTACCGTTCTGCAAGTGTTGAAGGTTCTTCTTACTGCCGTGGCATCCACGAAGTTTAGGGTACATGGGGAACCTTTGCTGGGAGTTATCAGAGTGTGCTACAATATTGCTCTGCACAGCAAGAGTCCCATAAACCAAGCAACGTCAAAGGCAATGCTTACGCAGATGGTCAGCATCATATTCAGGAGAATGGAGACTGATCCGGGACTTGAGGTTGCATCATCTGGTTCTGGTGGACATATAGAAGCCATTTCAGGACAAACTTCAAATACTGAAGCCGAAGAAACTTCAAATACTGAAGCCGAAGAAACTTCCTCAGAAGGACAAAGTGAGAAAGAAATGACTTTAGGAGATCAACTTAACCAGGTCAAAGATACACCCATTGCATCTGTTGAGGAACTTCATAATCTTGCTGGCGGTGCTGACATCAAGGGCTTAGAGGCTGTCCTGGACAAAGCTGTGCATCATGAAGATGGTAAAAAGATAACCAGAGGGATTGACCTCGAGAGCATGACCATAGTTCAACGTGATGCCTTACTGGTTTTCCGCACTCTTTGCAAGATGGGGATGAAGGAAGACAACAATGAAGTTACATTGAAGACGAGGATTTTGTCTCTTGAGCTGTTGCAGGGTTTATTGGAAGGGGTTGGCCATCCATTTACTAGGAACTTCCATTTTATTGACTCGGTGAAAGCATACCTTTCATATGCTTTGTTGCGGGCTTCAGTTTCCCAGTCTCCTGTCATATTTCAGTATGCAACAGGAATTTTTTTAGTGCTATTGTTGCGATTCAGAGAAAGCCTCAAGGGTGAAATAGGCATCTTCTTTCCCTTGATAGTTCTACGATCACTGGATGGCTTGGATTTTCCAATTAGCCAGAAATTAAGTGTTCTTAGGATGGTTGAAAAAGTTTGCAAGGATCCTCAGATGCTTGTTGACATATTTGTGAACTATGATTGTGATCTTGAGGCACCAAACTTGTTTGAGCGTATGGTTACAAGTCTATCCAGAATTTCTCAAGGGACTCAAAACACAGATCCAAATACGGTTGCTGTATCACAGGCAACCTCAATTAAAGGTTCATCTCTTCAGTGTCTCGTGAATGTGCTTAAATCACTAGTTGATTGGGAAAAGTCACGTGGAGAATCAGAAAACCAGAGTAATAAGACTCGGTCTCTAGATGGAGAAGCTAAAGAACCTGTTGATGTAACCAGTAACTTTGAGAAGGCAAAAGCTCATAAATCTACATTGGAAGCTGCCATCTCTGAGTTCAATAGGCAACCAGTAAAGGGTGTAGAATATCTGAAATCCAATAAATTGGTGGAAAATACACCCAGTTCGGTTGCCCAATTTTTGAGAAGTACTCCCAGTCTGGACAAGGCTATGATTGGGGAATATTTGGGTCACCATGAAGAATTTCCCCTTGCTGTGATGCATGCTTATGTAGATTCCATGAAGTTTTCAGGAATGAAGTTTGACACTGCAATTCGTGAACTTCTTAAAGGATTCAGACTTCCAGGGGAAGCTCAAAAGATAGATCGCATCATGGAAAAATTTGCAGAGAGATATTGTGCAGATAATCCAGGTCTTTTCAAAAATGCAGACACCGCATACATTTTAGCATATGCGGTTATAATGTTGAATACTGATGCGCATAATCCAATGGTGTGGCCTAAAATGTCCAAGTCAGATTTTGTACGCATGAATGCTGTAGATGACGCAGAAGAGTGTGCTCCAACTGAACTTCTGGAAGAAATCTACGATTCAATTGTTAAAGAAGAGATAAAGATGAAAGATGAGACTGCTGGTCTTGAGAAAAGTGGAAAATATAAGCCAGAAGGTGAAGAGAGAGGCCGCCTTGTCAGTATCCTTAATCTGGCTCTTCCCAGAAGTGCTTTGTCCGTTGATACTAAGTCTGAAAGTGAAGCTATCATTAAGAAAACACAAGCTATATTTCGGAATCAAGGAGCAAAAAGAGGGGTCTTCTATACTACACAACAATTAGAGCTAGTAAGGCCTATGGTTGAAGCTGTAGGATGGCCTTTGCTCGCTACATTTTCTGTTTCAATGGAGGAGGGTGAGAATAAGTCGAGGGTTGTTCTCTGTATGGAGGGATTTAAAGCTGGAATACACATTACACATGTTCTTGGAATGAATACCATGCGCTATGCCTTTTTAACATCTTTGGTCAGATTTACTTTCTTGCATGCTCCGAAGGAAATGCGTAGTAAAAATGCAGAAGCACTGCGAACTCTACTCTCTCTTTGTGATTCAGAGACTGGCTCCCTTAGAGATACATGGAATGCAGTTTTAGAATGTGTTTCTCGGCTTGAGTTCATTACTTCAACTCCTTCTATAGCAGCAACAGTCATGCAAGGATCAAACCAAATCTCCAAAGACGCTCTTCTTCAATCTTTAAGAGAGTTGGCTGGAAAACCTTCTGAACAAGTATTTGTGAATAGCGTTCAGCTTCCTAGTGATTCTGTAGTGGAGTTCTTCACTGCACTCTGTGGCGTATCAGCTGAAGAACTAAAACAAACTCCAGCTCGTGTGTTTAGCTTGCAAAAACTTGTAGAGATCAGCTACTACAACATGGCTCGTATACGCATGGTCTGGGCTAGAATATGGTCTGTTTTGGCATACCACTTTATTTCAGCTGGGAGTCATCGTGATGAAAAAATTGCTATGTATGCAATAGATTCTCTGAGGCAGCTTGGTATGAAGTATCTGGAGCGAGCGGAACTTGCCAATTTCACATTCCAAAATGACATTCTTAAACCTTTTGTTGTTCTTATGCGCAATAGTCGAAGTGAAACCATAAGGAGCTTAATAGTTGATTGCATTGTTCAAATGATAAAATCCAAAGTAGGGAGCATCAAGTCTGGATGGCGTAGTGTTTTCATGATTTTTACAGCTGCTGCAGATGATGAATTGGAATCAATCGTTGAGAGTGCATTTGAAAATGTTGAACAGGTTATCTTGGAACACTTCGATCAAGTAGTTGGAGATTGTTTCATGGACTGTGTCAACTGTCTTATCAGGTTTGCTAATAATAGAACTTCACACCGTATAAGTTTGAAGGCTATTGCACTCTTGCGGATATGTGAGGATCGTCTGGCAGAGGGTCTTATACCTGGTGGTGCCTTGAGGCCTCTTGATGTAAATATGGATACAACTTTTGATGTGACCGAACATTATTGGTTCCCAATGCTGGCTGGTCTGTCTGATCTGACATCGGATCCAAGACCAGAAGTCAGAAGCTGTGCACTTGAAGTTTTGTTTGATCTACTGAATGAGAGAGGTACCAAGTTCTCATCAACATTTTGGGAGAGCATTTTTCATCGGGTCTTGTTTCCCATATTTGATCACGTGAGACATGCTGGAAAGGAAAGCTTGGTTTCTTCTGACGATGAATGGTTCCGTGAAACAAGCATTCACTCGCTGCAGTTGCTTTGCAACCTTTTCAACACGTTTTATAAGGAGGTATGTTTCATGTTGCCACCCCTACTCAGTCTACTTTTGGATTGTGCCAAAAAGACTGATCAAACAGTAGTTTCATTATCTCTGGGTGCACTGGTGCATCTCATTGAAGTTGGAGGACATCAATTTAGTGAGAGCGACTGGGACACATTGTTGAAAAGCATAAGAGATGCTTTGTACACAACGCAACCACTTGAACTGCTAAACGCTTTGGGATTTGAGAATCTGAAGAACAATAATAGGGTGCTAATCGGAGATTTAGAGGTCAATTCAGGTGATAGCCCTTCCATAAAGTGTGATTATGACGGAGTGGATAGCCGTCAATTTGATGTCAGTGATAATGGAAGAAATCCAAATGCACCGGTGTTGGCCAAACAAAACTCGGGGGTGCAAATGAAGACGGATGGATCTGAAGGCCTTCCATCACCATCGGGAAGTGCTTCAAAATCTGCTGAAGCTGGAAGCCTCCAACGGACTCAAACAATAGGTCAAAGAATTATGGACAATCTGTTTCTCAGAAATCTTTCTAAGCCTAAGGCTATTCCATCCGATGCTTCAGTACCATCTTCTCCAATTAGGGCGCCTGAGGCTGTCGAACCTGATATCAGAGATGAGGAGGAGAGTTCACTGTTGGGAACTTGTAGGAGCAAATGCATCACTCAACTTTTACTGCTTGGCGCCATTGACAGTATTCAGAAGAAATACTGGAGCAAGTTGAAAGCACCACAAAAGATTGCTATTATGGACATATTGCTGTCTGCATTAGAGTTCGCCGCTTCCTATAATTCATATACCAATCTCAGAACACGCATGCACCAAATTCCTGATGAGAGGCCACCTCTGAATCTTCTTCGCCAAGAATTAGCTGGAACTGTCATATATCTGGAGATCTTACAGAAGGCAACCTCTGGGGTTAGTGCCGACAAAGAGGGAGAAACTGATGGTGAAGAGAAAGTCGAAGGATTAGCAGAAGAGAAACTGGTGTCTTTCTGTGAACAGGTACTCAGGGAGGCATCTGATCTGCAGTCCGGTTCTGGCGAGACTACTAATATGGATATTCATCGGGTTCTGGAGTTACGATCTCCGATAATTATCAAGGTGCTTAAAGGCATGTGCTTTATGAACCAACAAATTTTCAGAAGGCATCTGAGAGACTTCTATCCGTTGCTTACAAAACTCGTATGCTGTGACCAGATGGATGTTCGCGGAGCTCTTGGTGATTTATTCAGGGCACAATTGAAAGCACTTCTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1747

Amino Acids

193.75

Weight (kDa)

5.33

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 94 - 235 2.5e-26 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 343 - 495 8.5e-40 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 582 - 763 1e-71 Sec7 domain
Sec7-like_HDS PF09324 1076 - 1158 3.6e-27 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1232 - 1295 3.6e-07 C-terminal region of Mon2 protein
BIG2_C PF20252 1548 - 1737 1.3e-26 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2138, 3981
Acc16I TGCGCA 2 cut(s) 2157, 3302
Acc36I ACCTGC 1 cut(s) 373
Acc65I GGTACC 1 cut(s) 3806
AccB1I GGYRCC 5 cut(s) 1474, 3644, 3806, 4564, 4661
AccBSI CCGCTC 1 cut(s) 3242
AccI GTMKAC 2 cut(s) 3106, 4018
AccII CGCG 1 cut(s) 5190
AcoI YGGCCR 2 cut(s) 1153, 4353
AcsI RAATTY 6 cut(s) 1269, 1515, 1919, 2057, 4803, 5115
AcvI CACGTG 2 cut(s) 1643, 3874
AcyI GRCGYC 2 cut(s) 4565, 4662
AdeI CACNNNGTG 1 cut(s) 3014
AfiI CCNNNNNNNGG 6 cut(s) 271, 584, 1793, 2380, 3372, 3715
AflIII ACRYGT 2 cut(s) 2644, 3972
AgeI ACCGGT 1 cut(s) 4345
AhdI GACNNNNNGTC 2 cut(s) 2310, 3755
AhlI ACTAGT 1 cut(s) 1621
AjnI CCWGG 7 cut(s) 190, 418, 867, 951, 2020, 2086, 3637
AjuI GAANNNNNNNTTGG 4 cut(s) 3245, 3258, 3277, 3290
Alw21I GWGCWC 6 cut(s) 17, 298, 2239, 3772, 4078, 5197
Alw44I GTGCAC 2 cut(s) 3768, 4074
AlwNI CAGNNNCTG 3 cut(s) 2783, 3422, 3765
Ama87I CYCGRG 2 cut(s) 1007, 4366
ApaLI GTGCAC 2 cut(s) 3768, 4074
ApeKI GCWGC 7 cut(s) 170, 1760, 2864, 3214, 3419, 3422, 3946
ApoI RAATTY 6 cut(s) 1269, 1515, 1919, 2057, 4803, 5115
ArsI GACNNNNNNTTYG 4 cut(s) 1096, 1128, 4456, 4488
AsiGI ACCGGT 1 cut(s) 4345
Asp700I GAANNNNTTC 5 cut(s) 1920, 2941, 4401, 4423, 4507
Asp718I GGTACC 1 cut(s) 3806
AspLEI GCGC 5 cut(s) 2158, 2670, 3303, 4567, 4664
AsuC2I CCSGG 2 cut(s) 277, 708
AvaI CYCGRG 2 cut(s) 1007, 4366
AxyI CCTNAGG 2 cut(s) 4518, 4569
BaeGI GKGCMC 3 cut(s) 3772, 4078, 5218
BalI TGGCCA 2 cut(s) 1155, 4355
BamHI GGATCC 2 cut(s) 1423, 3742
BanI GGYRCC 5 cut(s) 1474, 3644, 3806, 4564, 4661
BanII GRGCYC 2 cut(s) 298, 5197
BauI CACGAG 2 cut(s) 1601, 3051
BbrPI CACGTG 2 cut(s) 1643, 3874
BbsI GAAGAC 5 cut(s) 50, 1086, 1109, 2479, 4388
Bbv12I GWGCWC 6 cut(s) 17, 298, 2239, 3772, 4078, 5197
BbvI GCAGC 7 cut(s) 157, 1747, 2876, 3226, 3406, 3409, 3933
BceAI ACGGC 2 cut(s) 527, 4287
BcgI CGANNNNNNTGC 2 cut(s) 2707, 2741
BciT130I CCWGG 7 cut(s) 192, 420, 869, 953, 2022, 2088, 3639
BciVI GTATCC 2 cut(s) 2381, 3667
BclI TGATCA 3 cut(s) 400, 3868, 4045
BcnI CCSGG 2 cut(s) 277, 708
BcuI ACTAGT 1 cut(s) 1621
BfaI CTAG 9 cut(s) 266, 359, 425, 1167, 1622, 1682, 2513, 2979, 3123
BfmI CTRYAG 7 cut(s) 2214, 2535, 2859, 2988, 3423, 3947, 5006
BfoI RGCGCY 2 cut(s) 4568, 4665
BfuAI ACCTGC 1 cut(s) 373
BfuI GTATCC 2 cut(s) 2381, 3667
BglI GCCNNNNNGGC 1 cut(s) 233
BglII AGATCT 2 cut(s) 34, 4872
BisI GCNGC 9 cut(s) 171, 1761, 2359, 2865, 3215, 3420, 3423, 3947, 4758
BlsI GCNGC 9 cut(s) 172, 1762, 2360, 2866, 3216, 3421, 3424, 3948, 4759
BmcAI AGTACT 1 cut(s) 1867
Bme1390I CCNGG 9 cut(s) 192, 277, 420, 708, 869, 953, 2022, 2088, 3639
BmeRI GACNNNNNGTC 2 cut(s) 2310, 3755
BmeT110I CYCGRG 2 cut(s) 1007, 4366
BmrFI CCNGG 9 cut(s) 192, 277, 420, 708, 869, 953, 2022, 2088, 3639
BmrI ACTGGG 4 cut(s) 1231, 1833, 1866, 4135
BmuI ACTGGG 4 cut(s) 1231, 1833, 1866, 4135
BpiI GAAGAC 5 cut(s) 50, 1086, 1109, 2479, 4388
BplI GAGNNNNNCTC 8 cut(s) 1290, 1322, 1852, 1884, 2771, 2803, 4807, 4839
BpmI CTGGAG 5 cut(s) 3030, 3254, 4711, 4889, 5075
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 8 cut(s) 734, 1142, 1292, 1490, 1506, 2336, 2855, 3670
BpuMI CCSGG 2 cut(s) 277, 708
BsaAI YACGTR 2 cut(s) 1643, 3874
BsaBI GATNNNNATC 3 cut(s) 1464, 5043, 5075
BsaHI GRCGYC 2 cut(s) 4565, 4662
BsaI GGTCTC 1 cut(s) 1683
BsaJI CCNNGG 2 cut(s) 543, 2021
BsaWI WCCGGW 2 cut(s) 4345, 5012
BsaXI ACNNNNNCTCC 8 cut(s) 203, 233, 2836, 2866, 2879, 2909, 3157, 3187
Bsc4I CCNNNNNNNGG 6 cut(s) 271, 584, 1793, 2380, 3372, 3715
Bse118I RCCGGY 2 cut(s) 232, 4345
Bse21I CCTNAGG 2 cut(s) 4518, 4569
Bse3DI GCAATG 3 cut(s) 390, 663, 885
Bse8I GATNNNNATC 3 cut(s) 1464, 5043, 5075
BseBI CCWGG 7 cut(s) 192, 420, 869, 953, 2022, 2088, 3639
BseDI CCNNGG 2 cut(s) 543, 2021
BseJI GATNNNNATC 3 cut(s) 1464, 5043, 5075
BseLI CCNNNNNNNGG 6 cut(s) 271, 584, 1793, 2380, 3372, 3715
BseMI GCAATG 3 cut(s) 390, 663, 885
BseRI GAGGAG 4 cut(s) 224, 2591, 4613, 4616
BseSI GKGCMC 3 cut(s) 3772, 4078, 5218
BseXI GCAGC 7 cut(s) 157, 1747, 2876, 3226, 3406, 3409, 3933
BseYI CCCAGC 2 cut(s) 583, 3161
BsgI GTGCAG 3 cut(s) 602, 2097, 2991
Bsh1236I CGCG 1 cut(s) 5190
BshNI GGYRCC 5 cut(s) 1474, 3644, 3806, 4564, 4661
BshTI ACCGGT 1 cut(s) 4345
BsiHKAI GWGCWC 6 cut(s) 17, 298, 2239, 3772, 4078, 5197
BsiHKCI CYCGRG 2 cut(s) 1007, 4366
BsiSI CCGG 5 cut(s) 233, 276, 707, 4346, 5013
BslFI GGGAC 4 cut(s) 615, 723, 1540, 4142
BslI CCNNNNNNNGG 6 cut(s) 271, 584, 1793, 2380, 3372, 3715
BsmFI GGGAC 4 cut(s) 615, 723, 1540, 4142
BsmI GAATGC 3 cut(s) 2215, 2812, 3936
Bso31I GGTCTC 1 cut(s) 1683
BsoBI CYCGRG 2 cut(s) 1007, 4366
Bsp1286I GDGCHC 7 cut(s) 17, 298, 2239, 3772, 4078, 5197, 5218
Bsp1407I TGTACA 1 cut(s) 4161
BspFNI CGCG 1 cut(s) 5190
BspHI TCATGA 2 cut(s) 970, 3405
BspMAI CTGCAG 3 cut(s) 3427, 3951, 5010
BspMI ACCTGC 1 cut(s) 373
BspQI GCTCTTC 2 cut(s) 2391, 2913
BspT107I GGYRCC 5 cut(s) 1474, 3644, 3806, 4564, 4661
BspTNI GGTCTC 1 cut(s) 1683
BsrBI CCGCTC 1 cut(s) 3242
BsrDI GCAATG 3 cut(s) 390, 663, 885
BsrFI RCCGGY 2 cut(s) 232, 4345
BsrGI TGTACA 1 cut(s) 4161
BssAI RCCGGY 2 cut(s) 232, 4345
BssECI CCNNGG 2 cut(s) 543, 2021
BssNAI GTATAC 1 cut(s) 3107
BssNI GRCGYC 2 cut(s) 4565, 4662
BssSI CACGAG 2 cut(s) 1601, 3051
Bst1107I GTATAC 1 cut(s) 3107
Bst2BI CACGAG 2 cut(s) 1601, 3051
Bst2UI CCWGG 7 cut(s) 192, 420, 869, 953, 2022, 2088, 3639
Bst6I CTCTTC 8 cut(s) 1596, 2223, 2277, 2343, 2391, 2913, 4926, 4950
BstACI GRCGYC 2 cut(s) 4565, 4662
BstAPI GCANNNNNTGC 1 cut(s) 1059
BstAUI TGTACA 1 cut(s) 4161
BstBAI YACGTR 2 cut(s) 1643, 3874
BstDSI CCRYGG 1 cut(s) 543
BstEII GGTNACC 1 cut(s) 1907
BstENI CCTNNNNNAGG 1 cut(s) 269
BstFNI CGCG 1 cut(s) 5190
BstH2I RGCGCY 2 cut(s) 4568, 4665
BstHHI GCGC 5 cut(s) 2158, 2670, 3303, 4567, 4664
BstNI CCWGG 7 cut(s) 192, 420, 869, 953, 2022, 2088, 3639
BstNSI RCATGY 7 cut(s) 32, 1943, 2648, 2714, 3884, 4798, 5098
BstPI GGTNACC 1 cut(s) 1907
BstSCI CCNGG 9 cut(s) 190, 275, 418, 706, 867, 951, 2020, 2086, 3637
BstSFI CTRYAG 7 cut(s) 2214, 2535, 2859, 2988, 3423, 3947, 5006
BstSLI GKGCMC 3 cut(s) 3772, 4078, 5218
BstUI CGCG 1 cut(s) 5190
BstV1I GCAGC 7 cut(s) 157, 1747, 2876, 3226, 3406, 3409, 3933
BstV2I GAAGAC 5 cut(s) 50, 1086, 1109, 2479, 4388
BstX2I RGATCY 7 cut(s) 34, 415, 1423, 1540, 3742, 4391, 4872
BstXI CCANNNNNNTGG 2 cut(s) 1822, 2172
BstYI RGATCY 7 cut(s) 34, 415, 1423, 1540, 3742, 4391, 4872
BstZ17I GTATAC 1 cut(s) 3107
Bsu36I CCTNAGG 2 cut(s) 4518, 4569
BsuI GTATCC 2 cut(s) 2381, 3667
BtgI CCRYGG 1 cut(s) 543
BtsI GCAGTG 3 cut(s) 1983, 2747, 3003
BtsIMutI CAGTG 8 cut(s) 1352, 1601, 1983, 2747, 3003, 4076, 4324, 4610
BveI ACCTGC 1 cut(s) 373
CaiI CAGNNNCTG 3 cut(s) 2783, 3422, 3765
CciI TCATGA 2 cut(s) 970, 3405
CfoI GCGC 5 cut(s) 2158, 2670, 3303, 4567, 4664
Cfr10I RCCGGY 2 cut(s) 232, 4345
CseI GACGC 2 cut(s) 2231, 2912
CsiI ACCWGGT 3 cut(s) 190, 867, 3637
CspAI ACCGGT 1 cut(s) 4345
DinI GGCGCC 2 cut(s) 4566, 4663
DraI TTTAAA 1 cut(s) 2623
DraIII CACNNNGTG 1 cut(s) 3014
DriI GACNNNNNGTC 2 cut(s) 2310, 3755
EaeI YGGCCR 2 cut(s) 1153, 4353
Eam1104I CTCTTC 8 cut(s) 1596, 2223, 2277, 2343, 2391, 2913, 4926, 4950
Eam1105I GACNNNNNGTC 2 cut(s) 2310, 3755
EarI CTCTTC 8 cut(s) 1596, 2223, 2277, 2343, 2391, 2913, 4926, 4950
Ecl136II GAGCTC 2 cut(s) 296, 5195
Eco147I AGGCCT 3 cut(s) 2521, 3655, 4401
Eco24I GRGCYC 2 cut(s) 298, 5197
Eco31I GGTCTC 1 cut(s) 1683
Eco32I GATATC 1 cut(s) 4588
Eco53kI GAGCTC 2 cut(s) 296, 5195
Eco72I CACGTG 2 cut(s) 1643, 3874
Eco81I CCTNAGG 2 cut(s) 4518, 4569
Eco88I CYCGRG 2 cut(s) 1007, 4366
Eco91I GGTNACC 1 cut(s) 1907
EcoICRI GAGCTC 2 cut(s) 296, 5195
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoO65I GGTNACC 1 cut(s) 1907
EcoRII CCWGG 7 cut(s) 190, 418, 867, 951, 2020, 2086, 3637
EcoRV GATATC 1 cut(s) 4588
EcoT22I ATGCAT 2 cut(s) 1941, 4640
EcoT38I GRGCYC 2 cut(s) 298, 5197
EgeI GGCGCC 2 cut(s) 4566, 4663
EheI GGCGCC 2 cut(s) 4566, 4663
FalI AAGNNNNNCTT 8 cut(s) 1188, 1220, 2892, 2924, 3881, 3913, 4141, 4173
FaqI GGGAC 4 cut(s) 615, 723, 1540, 4142
FauI CCCGC 1 cut(s) 1215
FauNDI CATATG 3 cut(s) 397, 1210, 2128
FbaI TGATCA 3 cut(s) 400, 3868, 4045
FblI GTMKAC 2 cut(s) 3106, 4018
Fnu4HI GCNGC 9 cut(s) 171, 1761, 2359, 2865, 3215, 3420, 3423, 3947, 4758
FriOI GRGCYC 2 cut(s) 298, 5197
Fsp4HI GCNGC 9 cut(s) 171, 1761, 2359, 2865, 3215, 3420, 3423, 3947, 4758
FspAI RTGCGCAY 1 cut(s) 2157
FspBI CTAG 9 cut(s) 266, 359, 425, 1167, 1622, 1682, 2513, 2979, 3123
FspI TGCGCA 2 cut(s) 2157, 3302
GlaI GCGC 5 cut(s) 2157, 2669, 3302, 4566, 4663
GluI GCNGC 9 cut(s) 171, 1761, 2359, 2865, 3215, 3420, 3423, 3947, 4758
GsaI CCCAGC 2 cut(s) 587, 3165
GsuI CTGGAG 5 cut(s) 3030, 3254, 4711, 4889, 5075
HaeII RGCGCY 2 cut(s) 4568, 4665
HapII CCGG 5 cut(s) 233, 276, 707, 4346, 5013
HgaI GACGC 2 cut(s) 2231, 2912
HhaI GCGC 5 cut(s) 2158, 2670, 3303, 4567, 4664
Hin1I GRCGYC 2 cut(s) 4565, 4662
Hin6I GCGC 5 cut(s) 2156, 2668, 3301, 4565, 4662
HinP1I GCGC 5 cut(s) 2156, 2668, 3301, 4565, 4662
HincII GTYRAC 4 cut(s) 82, 487, 1441, 3532
HindII GTYRAC 4 cut(s) 82, 487, 1441, 3532
HindIII AAGCTT 1 cut(s) 3895
HpaII CCGG 5 cut(s) 233, 276, 707, 4346, 5013
HpyCH4IV ACGT 5 cut(s) 647, 1030, 1642, 3873, 3974
HpySE526I ACGT 5 cut(s) 647, 1030, 1642, 3873, 3974
Hsp92I GRCGYC 2 cut(s) 4565, 4662
HspAI GCGC 5 cut(s) 2156, 2668, 3301, 4565, 4662
KasI GGCGCC 2 cut(s) 4564, 4661
KpnI GGTACC 1 cut(s) 3810
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Ksp22I TGATCA 3 cut(s) 400, 3868, 4045
LguI GCTCTTC 2 cut(s) 2391, 2913
Lsp1109I GCAGC 7 cut(s) 157, 1747, 2876, 3226, 3406, 3409, 3933
MabI ACCWGGT 3 cut(s) 190, 867, 3637
MaeI CTAG 9 cut(s) 266, 359, 425, 1167, 1622, 1682, 2513, 2979, 3123
MaeII ACGT 5 cut(s) 647, 1030, 1642, 3873, 3974
MaeIII GTNAC 9 cut(s) 1093, 1498, 1638, 1711, 1718, 1907, 3688, 5058, 5171
MbiI CCGCTC 1 cut(s) 3242
MfeI CAATTG 2 cut(s) 2271, 5219
MflI RGATCY 7 cut(s) 34, 415, 1423, 1540, 3742, 4391, 4872
MhlI GDGCHC 7 cut(s) 17, 298, 2239, 3772, 4078, 5197, 5218
MlsI TGGCCA 2 cut(s) 1155, 4355
MluNI TGGCCA 2 cut(s) 1155, 4355
Mly113I GGCGCC 2 cut(s) 4565, 4662
MlyI GAGTC 8 cut(s) 82, 227, 637, 1181, 1522, 1666, 3175, 4453
MmeI TCCRAC 5 cut(s) 315, 2264, 3487, 4078, 4477
Mox20I TGGCCA 2 cut(s) 1155, 4355
Mph1103I ATGCAT 2 cut(s) 1941, 4640
MroNI GCCGGC 1 cut(s) 232
MroXI GAANNNNTTC 5 cut(s) 1920, 2941, 4401, 4423, 4507
MscI TGGCCA 2 cut(s) 1155, 4355
MslI CAYNNNNRTG 4 cut(s) 975, 1448, 1944, 3174
Msp20I TGGCCA 2 cut(s) 1155, 4355
MspA1I CMGCKG 5 cut(s) 134, 448, 3026, 3161, 3419
MspI CCGG 5 cut(s) 233, 276, 707, 4346, 5013
MspR9I CCNGG 9 cut(s) 192, 277, 420, 708, 869, 953, 2022, 2088, 3639
MunI CAATTG 2 cut(s) 2271, 5219
Mva1269I GAATGC 3 cut(s) 2215, 2812, 3936
MvaI CCWGG 7 cut(s) 192, 420, 869, 953, 2022, 2088, 3639
MvnI CGCG 1 cut(s) 5190
NaeI GCCGGC 1 cut(s) 234
NarI GGCGCC 2 cut(s) 4565, 4662
NciI CCSGG 2 cut(s) 277, 708
NdeI CATATG 3 cut(s) 397, 1210, 2128
NgoMIV GCCGGC 1 cut(s) 232
NmeAIII GCCGAG 1 cut(s) 2809
NmuCI GTSAC 4 cut(s) 1638, 1907, 3688, 5171
NsbI TGCGCA 2 cut(s) 2157, 3302
NsiI ATGCAT 2 cut(s) 1941, 4640
NspI RCATGY 7 cut(s) 32, 1943, 2648, 2714, 3884, 4798, 5098
PaeI GCATGC 4 cut(s) 32, 1943, 2714, 4798
PaeR7I CTCGAG 1 cut(s) 1007
PagI TCATGA 2 cut(s) 970, 3405
PceI AGGCCT 3 cut(s) 2521, 3655, 4401
PciI ACATGT 1 cut(s) 2644
PciSI GCTCTTC 2 cut(s) 2391, 2913
PctI GAATGC 3 cut(s) 2215, 2812, 3936
PdiI GCCGGC 1 cut(s) 234
PdmI GAANNNNTTC 5 cut(s) 1920, 2941, 4401, 4423, 4507
PflFI GACNNNGTC 2 cut(s) 1675, 3527
PfoI TCCNGGA 2 cut(s) 706, 951
PinAI ACCGGT 1 cut(s) 4345
PkrI GCNGC 9 cut(s) 172, 1762, 2360, 2866, 3216, 3421, 3424, 3948, 4759
PleI GAGTC 8 cut(s) 82, 226, 636, 1181, 1522, 1666, 3174, 4453
PluTI GGCGCC 2 cut(s) 4568, 4665
PmaCI CACGTG 2 cut(s) 1643, 3874
PmlI CACGTG 2 cut(s) 1643, 3874
PpsI GAGTC 8 cut(s) 82, 226, 636, 1181, 1522, 1666, 3174, 4453
Ppu21I YACGTR 2 cut(s) 1643, 3874
PscI ACATGT 1 cut(s) 2644
PsiI TTATAA 2 cut(s) 2138, 3981
Psp124BI GAGCTC 2 cut(s) 298, 5197
Psp6I CCWGG 7 cut(s) 190, 418, 867, 951, 2020, 2086, 3637
PspCI CACGTG 2 cut(s) 1643, 3874
PspEI GGTNACC 1 cut(s) 1907
PspFI CCCAGC 2 cut(s) 583, 3161
PspGI CCWGG 7 cut(s) 190, 418, 867, 951, 2020, 2086, 3637
PstI CTGCAG 3 cut(s) 3427, 3951, 5010
PstNI CAGNNNCTG 3 cut(s) 2783, 3422, 3765
PsuI RGATCY 7 cut(s) 34, 415, 1423, 1540, 3742, 4391, 4872
PsyI GACNNNGTC 2 cut(s) 1675, 3527
PvuII CAGCTG 4 cut(s) 134, 3026, 3161, 3419
RseI CAYNNNNRTG 4 cut(s) 975, 1448, 1944, 3174
SacI GAGCTC 2 cut(s) 298, 5197
SapI GCTCTTC 2 cut(s) 2391, 2913
SatI GCNGC 9 cut(s) 171, 1761, 2359, 2865, 3215, 3420, 3423, 3947, 4758
ScaI AGTACT 1 cut(s) 1867
SchI GAGTC 8 cut(s) 82, 227, 637, 1181, 1522, 1666, 3175, 4453
ScrFI CCNGG 9 cut(s) 192, 277, 420, 708, 869, 953, 2022, 2088, 3639
SduI GDGCHC 7 cut(s) 17, 298, 2239, 3772, 4078, 5197, 5218
SexAI ACCWGGT 3 cut(s) 190, 867, 3637
SfcI CTRYAG 7 cut(s) 2214, 2535, 2859, 2988, 3423, 3947, 5006
SfoI GGCGCC 2 cut(s) 4566, 4663
Sfr274I CTCGAG 1 cut(s) 1007
SgrAI CRCCGGYG 1 cut(s) 4345
SlaI CTCGAG 1 cut(s) 1007
SmiMI CAYNNNNRTG 4 cut(s) 975, 1448, 1944, 3174
SpeI ACTAGT 1 cut(s) 1621
SphI GCATGC 4 cut(s) 32, 1943, 2714, 4798
SseBI AGGCCT 3 cut(s) 2521, 3655, 4401
SspDI GGCGCC 2 cut(s) 4564, 4661
SspI AATATT 2 cut(s) 610, 1901
SspMI CTAG 9 cut(s) 266, 359, 425, 1167, 1622, 1682, 2513, 2979, 3123
SstI GAGCTC 2 cut(s) 298, 5197
StuI AGGCCT 3 cut(s) 2521, 3655, 4401
StyD4I CCNGG 9 cut(s) 190, 275, 418, 706, 867, 951, 2020, 2086, 3637
TaiI ACGT 5 cut(s) 650, 1033, 1645, 3876, 3977
TaqI TCGA 6 cut(s) 1008, 2594, 3310, 3495, 4578, 4941
TaqII GACCGA 2 cut(s) 1665, 3707
TatI WGTACW 2 cut(s) 1865, 4161
TauI GCSGC 2 cut(s) 2361, 4760
TscAI CASTG 8 cut(s) 1359, 1601, 1990, 2754, 3010, 4083, 4324, 4617
TseFI GTSAC 4 cut(s) 1638, 1907, 3688, 5171
TseI GCWGC 7 cut(s) 170, 1760, 2864, 3214, 3419, 3422, 3946
Tsp45I GTSAC 4 cut(s) 1638, 1907, 3688, 5171
TspGWI ACGGA 7 cut(s) 211, 2395, 3914, 4305, 4400, 4471, 5136
TspRI CASTG 8 cut(s) 1359, 1601, 1990, 2754, 3010, 4083, 4324, 4617
Tth111I GACNNNGTC 2 cut(s) 1675, 3527
VneI GTGCAC 2 cut(s) 3768, 4074
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 6 cut(s) 1269, 1515, 1919, 2057, 4803, 5115
XbaI TCTAGA 2 cut(s) 424, 1681
XceI RCATGY 7 cut(s) 32, 1943, 2648, 2714, 3884, 4798, 5098
XcmI CCANNNNNNNNNTGG 2 cut(s) 1822, 4847
XhoI CTCGAG 1 cut(s) 1007
XmiI GTMKAC 2 cut(s) 3106, 4018
XmnI GAANNNNTTC 5 cut(s) 1920, 2941, 4401, 4423, 4507
XspI CTAG 9 cut(s) 266, 359, 425, 1167, 1622, 1682, 2513, 2979, 3123
ZrmI AGTACT 1 cut(s) 1867
Zsp2I ATGCAT 2 cut(s) 1941, 4640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.