FvH4_1g09670

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007281
Physical Location & Seq
Reverse (-)
5177519 .. 5190309
12791 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09670.t1

Sequence Viewer

Length: 3855 bp
ATGAACCAGAAAATGAGTGTTCTTAGGATGGTTGAAAAAGTTTGCAAGGATCCTCAGATGCTTGTAGACATATTTGTGAACTATGACTGTGATCTTGAGGCACCAAACTTGTTTGAGCGGATGGTCACAACTCTATCCAGAATATCTCAAGGGACGCAAAATGCAGATCCAAATATGGCTACTGCATCCCCAACTACTTCAATAAAAGGATCATCTCTTCAGTGTCTTGTGAATGTGCTTAAATCACTCGTAGATTGGGAAATGTCACGTGGAGAATCATATAACCAGAGTAAAAATGCTCAGTCTCTGGAAGGAGATGCTTCAGACAGAGAGTCTGTTGATGTGAAAAGCAGGCAAGATATGACCACTAACTTTGAGAAGGCAAAAGCTCATAAGTCTACACTGGAAGCTGCGATCTCTGAGTTCAACAGGCGACCGGTAAAGGGTGTAGAGTATCTAAGATCAAATAAGTTAGTGGAAAACACACCTAGTTCAGTTGCCCAATTTTTGAGAAGTACTCCCAGTTTGGACAAGGCTATGATTGGGGAATATTTGGGCCATCATGAAGAATTCCCGCTTTCTGTCATGCATGCTTATGTAGATTCCATGAAATTTTCGGGAATGAAGTTTGATACTGCAATTCGTGAACTTCTCAAAGGATTCCGACTTCCAGGGGAAGCTCAAAAGATAGATCGCATTATGGAAAAGTTTGCAGAAAGATACTGTGCAGATAATCCAGGCCTCTTCAAAAATGCAGATACGGCATATGTTCTTGCATATGCAGTTATTTTGTTGAATACTGATGCGCATAATCCAATGGTATGGCCTAAGATGTCCAAGTCAGATTTCATGCGCATGAATGCTACTGAAGATCCAGAAGATTGTGCTCCAAAAGAACTTCTGGAAGAAATCTATGACTCAATTGTTAAAGAAGAGATAAAGATGAAAGATGAGAGTACTAGTCTCGATAAAAGTGGCAAATATAAGCCAGAAGGTGAAGAGAGAGGACGCCTTGTCAGTATTCTTAATCTGGCTCTTCCCAGAAGAACGGTGTCTTCTGATACCAAGTCTGAAAGTGAAGCTATCATAAAGAGAGCACAAGATATATTTAGGAATCAAGGAGCAAAAAGAGGGGTCTTCCATACTACACAGCAAATAGAACTTGTAAGGCCTATGGTAGAAGCTGTTGGATGGCCTTTGCTTGCTACCTTTTCTGTTACAATGGAAGAGGGTGATAATAAGTCGAGGATTGTTCTCTGCATGGAAGGATTTAAAGCAGGAATACACATTACACACGTTCTGGGAATGGACACCATGCGCTATGCATTCTTAACATCTCTCGTCAGATTTACATTCTTGCATGCTCCAAAGGAAATGCGTAGTAAAAATGTGGAAGCATTGCGGACTCTACTAGCTCTTTGTGACTCAGAGACTGGGTCCCTTCAAGACACTTGGAATGCAGTTTTGGAATGTGTTTCGCGGCTTGAATTCATTAGTTCAACACCTGCTATTGCTGCAACAGTCATGCAGGGATCAAACCAGATCTCAAAAGATGCTGTTCTTCAATCTCTAAGAGAATTGGCAGGAAAACCTTCTGAGCAAGTATTTGTGAATAGTGTTCAGCTTCCCAGCGATTCTGTAGTGGAGTTCTTCACTGCACTCTGTGGTGTATCAGCTGAAGAACTAAAACAAAGTCCAGCTCGGGTTTTTAGCTTGCAGAAACTTGTTGAGATCAGCTACTACAACATGGCTCGTATACGCATGGTCTGGGCTAGAATATGGTCTGTTTTGGCAAACCACTTTATTTCAGCTGGGAGTCATCATGATGAGAAAATTGCTATGTATGCGATAGATTCTCTGAGGCAGCTTGGTATGAAGTATCTGGAGCGAGCTGAACTTGCAAATTTCACATTCCAAAATGACATTCTTAAGCCTTTCGTTGTACTTATGCGCAATAGTCGAAGTGAAACCATAAGGGGCCTAATTGTTGACTGCATTGTTCAAATGATAAAATCTAAAGTGGGGAGCATCAAGTCTGGTTGGCGTAGTGTTTTCATGATTTTTACAGCTTCTGCAGATGATGAATTGGAATCTATTGTTGAGAGTGCATTTGAAAATGTCGAACAAGTGATCTTGGAACACTTTGATCAAGTTGTTGGAGATTGTTTCATGGACTGTGTCAACTGTCTTATCCGATTCGCCAATAATAGAACTTCACACCGTATAAGTTTGAAGGCTATTGCACTCCTGCGCATATGTGAGGATCGTTTGGCTGAGGGTCTTATACCTGGTGGTGCTTTGAAGCCTATAGAAGATAATGATACAACAAACTTTGATGTGACTGAGCATTATTGGTTCCCAATGCTGGCTGGTCTGTCTGACCTGACATCAGATACAAGGCCAGAGGTCAGAAGCTGTGCGCTTGAAGTTTTATTTGATCTACTGAATGAGAGAGGTAACAAGTTCTCCTCATCCTTTTGGGAGAGCATCTTCCATCGTGTCTTGTTTCCCATATTTGACCATGTAAGACATGCTGGAAAGGAAAGCTCGGCTTCTTCTGATGAGGAATGGTTCCGTGAAACAAGCATTCACTCACTTCAGTTGCTGTGCAACCTTTTCAACACTTTTTACAAGGAGGTATGTTTCATGTTGCCACCACTACTCAGTCTACTGTTAGATTGTGCCAAAAAGACTGATCAAGCTGTGGTTTCGTTATCTCTGGGTGCACTGGTGCATCTAATTGAAGTTGGAGGACATCAATTTAGTGAGAGTGATTGGGATACATTATTGAAAAGCATAAGAGATGCTTTGTACACAACGCAACCACTTGAACTGTTAAATGCCTTGGGTTTTGAGAACCTGAAGAATGAGAGGACGTTAAATTTAGAGGTCAATTCCGGTGGTCCTTCTTTAATGTCTGACTATGATGGGGGAGACTATGATAGAAATCCAAATGCATCAGTTGAATCAGGGGTGCAAATGAACTTGGATGGATCTGAAGGCCTTAACTCACCGTCAGGAAGTGCTTCAAAATCTGCTGATGATGAAAACCTCCAACGTAGTCAAACAATTGGTCAAAGAATTATGGGTAACCTCTTTCTCAGAAATCTTTCATCTAAGCCCAAGAGTTCTGATGCTTCAGTACCATCTTCTCCAGTTAAGGTTGCTGATGTTGCTGAACCTGATATCAAAGATGAGGAGGAAAGCTCTGTATTGGGAACTTGTAGGGGCAAATGCATTACTCAATTATTACTTCTGGGAGCCATTGATAGTATCCAGAAGAAATACTGGAGCAAGTTGAAAGCACCACAGAAGATTGCTATATTGGACATTTTGTTGTCTGCATTAGAATTTGCAGCTTCCTACAATTCATATACAAATCTCAGAACACGCATGCACCAAATTTCTGATGAGAGGCCACCTTTGAATCTTCTTCGCCAAGAGTTAACAGGAACTTGCATATATCTGGACATCTTACAGAAAGCAACTTCCCAGTTTCCTGCCAATCAAGAGGGACTTGCAGAAACTAATGATTCAAGCGCTGAAGAGAATGTTGAAGGATTAGCGGAAGACAAACTTGTGTCTTTCTGCGAACAAGTACTCAGGGAGGCATCTGAGCTGCAGTCCAGTTCTGGAGATGTGACTAATATGGATATTCACCGTGTTCTGGAGTTACGATCTCCAATAATTGTTAAGGTGCTCAAAGGCATGTGCTTTATGAACCCACAGATTTTCAGAAGACATCTGAGAGACTTCTATCCTTTGCTTACAAAACTTGTATGCTGCGACCAGATGGATATTCGTGGAGCTCTCGGTGATCTGTTTAGGGCACAACTGAAAGCACTACTGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1285

Amino Acids

143.85

Weight (kDa)

5.37

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 2 - 37 2.1e-09 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 132 - 313 6.1e-71 Sec7 domain
Sec7-like_HDS PF09324 626 - 708 1.5e-26 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 782 - 855 1.5e-07 C-terminal region of Mon2 protein
BIG2_C PF20252 1081 - 1275 5.4e-27 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1513
Acc16I TGCGCA 4 cut(s) 807, 854, 1952, 2252
Acc36I ACCTGC 1 cut(s) 1513
AccB1I GGYRCC 1 cut(s) 100
AccBSI CCGCTC 1 cut(s) 118
AccI GTMKAC 4 cut(s) 66, 398, 1756, 2668
AccII CGCG 1 cut(s) 1480
AciI CCGC 5 cut(s) 118, 575, 1402, 1480, 3566
AclWI GGATC 8 cut(s) 44, 57, 161, 217, 866, 1540, 2271, 3001
AcsI RAATTY 7 cut(s) 569, 611, 1487, 1903, 2881, 3350, 3402
AcuI CTGAAG 9 cut(s) 203, 306, 888, 1698, 2582, 2882, 3018, 3123, 3564
AcvI CACGTG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 1009
AdeI CACNNNGTG 1 cut(s) 1664
AfaI GTAC 6 cut(s) 517, 958, 1944, 2813, 3144, 3600
AfeI AGCGCT 1 cut(s) 3541
AfiI CCNNNNNNNGG 3 cut(s) 443, 2365, 3667
AflII CTTAAG 1 cut(s) 1928
AflIII ACRYGT 1 cut(s) 1294
AgeI ACCGGT 1 cut(s) 436
AhdI GACNNNNNGTC 2 cut(s) 331, 1013
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 3 cut(s) 670, 736, 2287
AjuI GAANNNNNNNTTGG 6 cut(s) 830, 862, 1448, 1480, 1908, 1940
Alw21I GWGCWC 5 cut(s) 889, 1099, 2728, 3702, 3811
Alw26I GTCTC 5 cut(s) 309, 968, 1424, 2928, 3744
Alw44I GTGCAC 1 cut(s) 2724
AlwI GGATC 8 cut(s) 44, 57, 161, 217, 866, 1540, 2271, 3001
AlwNI CAGNNNCTG 5 cut(s) 307, 1433, 2072, 2415, 2605
Ama87I CYCGRG 1 cut(s) 1701
Aor51HI AGCGCT 1 cut(s) 3541
AoxI GGCC 9 cut(s) 556, 739, 824, 1169, 1193, 1978, 2399, 3001, 3416
ApaLI GTGCAC 1 cut(s) 2724
ApeKI GCWGC 6 cut(s) 410, 1514, 1864, 3356, 3619, 3783
ApoI RAATTY 7 cut(s) 569, 611, 1487, 1903, 2881, 3350, 3402
ArsI GACNNNNNNTTYG 2 cut(s) 3058, 3090
AsiGI ACCGGT 1 cut(s) 436
Asp700I GAANNNNTTC 3 cut(s) 1591, 3025, 3109
AspLEI GCGC 7 cut(s) 808, 855, 1320, 1953, 2253, 2422, 3542
AspS9I GGNCC 4 cut(s) 556, 1437, 1978, 2903
AsuHPI GGTGA 5 cut(s) 1007, 1244, 3003, 3650, 3827
AvaI CYCGRG 1 cut(s) 1701
AvaII GGWCC 2 cut(s) 1437, 2903
BaeGI GKGCMC 2 cut(s) 2728, 3832
BamHI GGATCC 1 cut(s) 49
BanI GGYRCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 3811
BbrPI CACGTG 1 cut(s) 269
BbsI GAAGAC 4 cut(s) 1047, 1129, 3576, 3745
Bbv12I GWGCWC 5 cut(s) 889, 1099, 2728, 3702, 3811
BbvCI CCTCAGC 1 cut(s) 2274
BbvI GCAGC 6 cut(s) 397, 1501, 1876, 3368, 3606, 3770
BccI CCATC 9 cut(s) 22, 115, 567, 1185, 2502, 2921, 2984, 3154, 3787
BceAI ACGGC 1 cut(s) 777
BciT130I CCWGG 3 cut(s) 672, 738, 2289
BciVI GTATCC 2 cut(s) 2773, 3284
BclI TGATCA 2 cut(s) 2146, 2695
BcoDI GTCTC 5 cut(s) 309, 968, 1424, 2928, 3744
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 5 cut(s) 489, 960, 1412, 1773, 3853
BfmI CTRYAG 4 cut(s) 1638, 2073, 2307, 3620
BfoI RGCGCY 1 cut(s) 3543
BfrI CTTAAG 1 cut(s) 1928
BfuAI ACCTGC 1 cut(s) 1513
BfuI GTATCC 2 cut(s) 2773, 3284
BglII AGATCT 1 cut(s) 1542
BisI GCNGC 7 cut(s) 411, 1481, 1515, 1865, 3357, 3620, 3784
BlsI GCNGC 7 cut(s) 412, 1482, 1516, 1866, 3358, 3621, 3785
BmcAI AGTACT 3 cut(s) 517, 958, 3600
Bme1390I CCNGG 3 cut(s) 672, 738, 2289
Bme18I GGWCC 2 cut(s) 1437, 2903
BmeRI GACNNNNNGTC 2 cut(s) 331, 1013
BmeT110I CYCGRG 1 cut(s) 1701
BmgT120I GGNCC 4 cut(s) 556, 1437, 1978, 2903
BmiI GGNNCC 8 cut(s) 51, 102, 1438, 1439, 1979, 2357, 2573, 3262
BmrFI CCNGG 3 cut(s) 672, 738, 2289
BmrI ACTGGG 3 cut(s) 516, 1443, 3487
BmuI ACTGGG 3 cut(s) 516, 1443, 3487
BpiI GAAGAC 4 cut(s) 1047, 1129, 3576, 3745
BplI GAGNNNNNCTC 4 cut(s) 502, 534, 3191, 3223
BpmI CTGGAG 5 cut(s) 1904, 3138, 3310, 3654, 3689
Bpu10I CCTNAGC 1 cut(s) 2274
BpuEI CTTGAG 2 cut(s) 116, 132
BsaAI YACGTR 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 2946
BsaHI GRCGYC 1 cut(s) 1009
BsaJI CCNNGG 2 cut(s) 671, 2844
BsaWI WCCGGW 2 cut(s) 436, 2897
BsaXI ACNNNNNCTCC 2 cut(s) 2450, 2480
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 2365, 3667
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 8 cut(s) 408, 522, 1438, 2733, 3155, 3293, 3493, 3627
Bse3DI GCAATG 1 cut(s) 1397
Bse8I GATNNNNATC 1 cut(s) 2946
BseBI CCWGG 3 cut(s) 672, 738, 2289
BseDI CCNNGG 2 cut(s) 671, 2844
BseGI GGATG 6 cut(s) 33, 126, 185, 1196, 2471, 2995
BseJI GATNNNNATC 1 cut(s) 2946
BseLI CCNNNNNNNGG 3 cut(s) 443, 2365, 3667
BseMI GCAATG 1 cut(s) 1397
BseNI ACTGG 8 cut(s) 408, 522, 1438, 2733, 3155, 3293, 3493, 3627
BseRI GAGGAG 2 cut(s) 2458, 3212
BseSI GKGCMC 2 cut(s) 2728, 3832
BseXI GCAGC 6 cut(s) 397, 1501, 1876, 3368, 3606, 3770
BseYI CCCAGC 2 cut(s) 1628, 1811
BsgI GTGCAG 2 cut(s) 747, 1641
Bsh1236I CGCG 1 cut(s) 1480
Bsh1285I CGRYCG 1 cut(s) 437
BshFI GGCC 9 cut(s) 558, 741, 826, 1171, 1195, 1980, 2401, 3003, 3418
BshNI GGYRCC 1 cut(s) 100
BshTI ACCGGT 1 cut(s) 436
BsiEI CGRYCG 1 cut(s) 437
BsiHKAI GWGCWC 5 cut(s) 889, 1099, 2728, 3702, 3811
BsiHKCI CYCGRG 1 cut(s) 1701
BsiSI CCGG 2 cut(s) 437, 2898
BslFI GGGAC 3 cut(s) 166, 1423, 3528
BslI CCNNNNNNNGG 3 cut(s) 443, 2365, 3667
BsmAI GTCTC 5 cut(s) 309, 968, 1424, 2928, 3744
BsmFI GGGAC 3 cut(s) 166, 1423, 3528
BsmI GAATGC 4 cut(s) 865, 1325, 1462, 2586
BsnI GGCC 9 cut(s) 558, 741, 826, 1171, 1195, 1980, 2401, 3003, 3418
BsoBI CYCGRG 1 cut(s) 1701
Bsp1286I GDGCHC 6 cut(s) 889, 1099, 2728, 3702, 3811, 3832
Bsp1407I TGTACA 1 cut(s) 2811
BspACI CCGC 5 cut(s) 118, 575, 1402, 1480, 3566
BspANI GGCC 9 cut(s) 558, 741, 826, 1171, 1195, 1980, 2401, 3003, 3418
BspFNI CGCG 1 cut(s) 1480
BspHI TCATGA 3 cut(s) 562, 1822, 2055
BspLI GGNNCC 8 cut(s) 51, 102, 1438, 1439, 1979, 2357, 2573, 3262
BspMAI CTGCAG 2 cut(s) 2077, 3624
BspMI ACCTGC 1 cut(s) 1513
BspPI GGATC 8 cut(s) 44, 57, 161, 217, 866, 1540, 2271, 3001
BspQI GCTCTTC 1 cut(s) 1041
BspT107I GGYRCC 1 cut(s) 100
BspTI CTTAAG 1 cut(s) 1928
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 1397
BsrFI RCCGGY 1 cut(s) 436
BsrGI TGTACA 1 cut(s) 2811
BsrI ACTGG 8 cut(s) 408, 522, 1438, 2733, 3155, 3293, 3493, 3627
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 2 cut(s) 671, 2844
BssNAI GTATAC 1 cut(s) 1757
BssNI GRCGYC 1 cut(s) 1009
BssT1I CCWWGG 1 cut(s) 2844
Bst1107I GTATAC 1 cut(s) 1757
Bst2UI CCWGG 3 cut(s) 672, 738, 2289
Bst6I CTCTTC 7 cut(s) 222, 749, 927, 993, 1041, 1221, 3540
BstACI GRCGYC 1 cut(s) 1009
BstAFI CTTAAG 1 cut(s) 1928
BstAUI TGTACA 1 cut(s) 2811
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 8 cut(s) 353, 591, 1203, 1362, 1715, 1890, 2367, 3395
BstEII GGTNACC 1 cut(s) 3089
BstF5I GGATG 6 cut(s) 33, 126, 185, 1196, 2471, 2995
BstFNI CGCG 1 cut(s) 1480
BstH2I RGCGCY 1 cut(s) 3543
BstHHI GCGC 7 cut(s) 808, 855, 1320, 1953, 2253, 2422, 3542
BstMAI GTCTC 5 cut(s) 309, 968, 1424, 2928, 3744
BstMCI CGRYCG 1 cut(s) 437
BstMWI GCNNNNNNNGC 5 cut(s) 761, 1514, 1844, 1898, 3173
BstNI CCWGG 3 cut(s) 672, 738, 2289
BstNSI RCATGY 5 cut(s) 593, 1364, 2534, 3397, 3712
BstPI GGTNACC 1 cut(s) 3089
BstSCI CCNGG 3 cut(s) 670, 736, 2287
BstSFI CTRYAG 4 cut(s) 1638, 2073, 2307, 3620
BstSLI GKGCMC 2 cut(s) 2728, 3832
BstUI CGCG 1 cut(s) 1480
BstV1I GCAGC 6 cut(s) 397, 1501, 1876, 3368, 3606, 3770
BstV2I GAAGAC 4 cut(s) 1047, 1129, 3576, 3745
BstX2I RGATCY 5 cut(s) 49, 166, 871, 1542, 2993
BstXI CCANNNNNNTGG 1 cut(s) 822
BstYI RGATCY 5 cut(s) 49, 166, 871, 1542, 2993
BstZ17I GTATAC 1 cut(s) 1757
BsuI GTATCC 2 cut(s) 2773, 3284
BsuRI GGCC 9 cut(s) 558, 741, 826, 1171, 1195, 1980, 2401, 3003, 3418
BtsCI GGATG 6 cut(s) 33, 126, 185, 1196, 2471, 2995
BtsI GCAGTG 1 cut(s) 1653
BtsIMutI CAGTG 4 cut(s) 227, 401, 1653, 2726
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 8 cut(s) 353, 591, 1203, 1362, 1715, 1890, 2367, 3395
CaiI CAGNNNCTG 5 cut(s) 307, 1433, 2072, 2415, 2605
CciI TCATGA 3 cut(s) 562, 1822, 2055
CfoI GCGC 7 cut(s) 808, 855, 1320, 1953, 2253, 2422, 3542
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 4 cut(s) 556, 1437, 1978, 2903
CseI GACGC 2 cut(s) 163, 1017
CsiI ACCWGGT 1 cut(s) 2287
Csp6I GTAC 6 cut(s) 516, 957, 1943, 2812, 3143, 3599
CspAI ACCGGT 1 cut(s) 436
CspCI CAANNNNNGTGG 4 cut(s) 355, 390, 2881, 2916
CviQI GTAC 6 cut(s) 516, 957, 1943, 2812, 3143, 3599
DraI TTTAAA 1 cut(s) 1273
DraIII CACNNNGTG 1 cut(s) 1664
DriI GACNNNNNGTC 2 cut(s) 331, 1013
Eam1104I CTCTTC 7 cut(s) 222, 749, 927, 993, 1041, 1221, 3540
Eam1105I GACNNNNNGTC 2 cut(s) 331, 1013
EarI CTCTTC 7 cut(s) 222, 749, 927, 993, 1041, 1221, 3540
Ecl136II GAGCTC 1 cut(s) 3809
Eco130I CCWWGG 1 cut(s) 2844
Eco147I AGGCCT 3 cut(s) 741, 1171, 3003
Eco24I GRGCYC 1 cut(s) 3811
Eco32I GATATC 1 cut(s) 3187
Eco47I GGWCC 2 cut(s) 1437, 2903
Eco47III AGCGCT 1 cut(s) 3541
Eco53kI GAGCTC 1 cut(s) 3809
Eco57I CTGAAG 9 cut(s) 203, 306, 888, 1698, 2582, 2882, 3018, 3123, 3564
Eco72I CACGTG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 1701
Eco91I GGTNACC 1 cut(s) 3089
EcoICRI GAGCTC 1 cut(s) 3809
EcoO109I RGGNCCY 2 cut(s) 1437, 1978
EcoO65I GGTNACC 1 cut(s) 3089
EcoRI GAATTC 2 cut(s) 569, 1487
EcoRII CCWGG 3 cut(s) 670, 736, 2287
EcoRV GATATC 1 cut(s) 3187
EcoT14I CCWWGG 1 cut(s) 2844
EcoT22I ATGCAT 4 cut(s) 591, 1327, 2959, 3239
EcoT38I GRGCYC 1 cut(s) 3811
ErhI CCWWGG 1 cut(s) 2844
FalI AAGNNNNNCTT 8 cut(s) 2536, 2568, 2791, 2823, 3501, 3533, 3561, 3593
FaqI GGGAC 3 cut(s) 166, 1423, 3528
FauI CCCGC 1 cut(s) 582
FauNDI CATATG 3 cut(s) 766, 778, 2255
FbaI TGATCA 2 cut(s) 2146, 2695
FblI GTMKAC 4 cut(s) 66, 398, 1756, 2668
Fnu4HI GCNGC 7 cut(s) 411, 1481, 1515, 1865, 3357, 3620, 3784
FokI GGATG 6 cut(s) 40, 133, 172, 1203, 2458, 3002
FriOI GRGCYC 1 cut(s) 3811
Fsp4HI GCNGC 7 cut(s) 411, 1481, 1515, 1865, 3357, 3620, 3784
FspAI RTGCGCAY 2 cut(s) 807, 854
FspBI CTAG 5 cut(s) 489, 960, 1412, 1773, 3853
FspI TGCGCA 4 cut(s) 807, 854, 1952, 2252
GlaI GCGC 7 cut(s) 807, 854, 1319, 1952, 2252, 2421, 3541
GluI GCNGC 7 cut(s) 411, 1481, 1515, 1865, 3357, 3620, 3784
GsaI CCCAGC 2 cut(s) 1632, 1815
GsuI CTGGAG 5 cut(s) 1904, 3138, 3310, 3654, 3689
HaeII RGCGCY 1 cut(s) 3543
HaeIII GGCC 9 cut(s) 558, 741, 826, 1171, 1195, 1980, 2401, 3003, 3418
HapII CCGG 2 cut(s) 437, 2898
HgaI GACGC 2 cut(s) 163, 1017
HhaI GCGC 7 cut(s) 808, 855, 1320, 1953, 2253, 2422, 3542
Hin1I GRCGYC 1 cut(s) 1009
Hin6I GCGC 7 cut(s) 806, 853, 1318, 1951, 2251, 2420, 3540
HinP1I GCGC 7 cut(s) 806, 853, 1318, 1951, 2251, 2420, 3540
HincII GTYRAC 3 cut(s) 1990, 2182, 3447
HindII GTYRAC 3 cut(s) 1990, 2182, 3447
HpaI GTTAAC 1 cut(s) 3447
HpaII CCGG 2 cut(s) 437, 2898
HphI GGTGA 5 cut(s) 1007, 1244, 3003, 3650, 3827
HpyCH4IV ACGT 4 cut(s) 268, 1296, 2876, 3058
HpyF10VI GCNNNNNNNGC 5 cut(s) 761, 1514, 1844, 1898, 3173
HpySE526I ACGT 4 cut(s) 268, 1296, 2876, 3058
Hsp92I GRCGYC 1 cut(s) 1009
HspAI GCGC 7 cut(s) 806, 853, 1318, 1951, 2251, 2420, 3540
KflI GGGWCCC 1 cut(s) 1437
Ksp22I TGATCA 2 cut(s) 2146, 2695
KspAI GTTAAC 1 cut(s) 3447
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 8 cut(s) 892, 1121, 1369, 1885, 2025, 3260, 3291, 3806
Lsp1109I GCAGC 6 cut(s) 397, 1501, 1876, 3368, 3606, 3770
MabI ACCWGGT 1 cut(s) 2287
MaeI CTAG 5 cut(s) 489, 960, 1412, 1773, 3853
MaeII ACGT 4 cut(s) 268, 1296, 2876, 3058
MaeIII GTNAC 9 cut(s) 124, 264, 1216, 1421, 2338, 2456, 3089, 3640, 3672
MbiI CCGCTC 1 cut(s) 118
MfeI CAATTG 2 cut(s) 921, 3069
MflI RGATCY 5 cut(s) 49, 166, 871, 1542, 2993
MhlI GDGCHC 6 cut(s) 889, 1099, 2728, 3702, 3811, 3832
MlyI GAGTC 5 cut(s) 341, 911, 1399, 1418, 1825
MmeI TCCRAC 5 cut(s) 688, 1168, 2137, 2728, 3079
Mph1103I ATGCAT 4 cut(s) 591, 1327, 2959, 3239
MroXI GAANNNNTTC 3 cut(s) 1591, 3025, 3109
MslI CAYNNNNRTG 4 cut(s) 74, 594, 854, 1824
MspA1I CMGCKG 2 cut(s) 1676, 1811
MspCI CTTAAG 1 cut(s) 1928
MspI CCGG 2 cut(s) 437, 2898
MspR9I CCNGG 3 cut(s) 672, 738, 2289
MunI CAATTG 2 cut(s) 921, 3069
Mva1269I GAATGC 4 cut(s) 865, 1325, 1462, 2586
MvaI CCWGG 3 cut(s) 672, 738, 2289
MvnI CGCG 1 cut(s) 1480
MwoI GCNNNNNNNGC 5 cut(s) 761, 1514, 1844, 1898, 3173
NdeI CATATG 3 cut(s) 766, 778, 2255
NlaIV GGNNCC 8 cut(s) 51, 102, 1438, 1439, 1979, 2357, 2573, 3262
NmeAIII GCCGAG 1 cut(s) 2528
NmuCI GTSAC 5 cut(s) 124, 264, 1421, 2338, 3640
NsbI TGCGCA 4 cut(s) 807, 854, 1952, 2252
NsiI ATGCAT 4 cut(s) 591, 1327, 2959, 3239
NspI RCATGY 5 cut(s) 593, 1364, 2534, 3397, 3712
PaeI GCATGC 3 cut(s) 593, 1364, 3397
PagI TCATGA 3 cut(s) 562, 1822, 2055
PaqCI CACCTGC 1 cut(s) 1513
PceI AGGCCT 3 cut(s) 741, 1171, 3003
PciSI GCTCTTC 1 cut(s) 1041
PctI GAATGC 4 cut(s) 865, 1325, 1462, 2586
PdmI GAANNNNTTC 3 cut(s) 1591, 3025, 3109
PflFI GACNNNGTC 2 cut(s) 1435, 2177
PinAI ACCGGT 1 cut(s) 436
PkrI GCNGC 7 cut(s) 412, 1482, 1516, 1866, 3358, 3621, 3785
PleI GAGTC 5 cut(s) 340, 911, 1399, 1418, 1824
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 5 cut(s) 340, 911, 1399, 1418, 1824
Ppu21I YACGTR 1 cut(s) 269
PpuMI RGGWCCY 1 cut(s) 1437
Psp124BI GAGCTC 1 cut(s) 3811
Psp5II RGGWCCY 1 cut(s) 1437
Psp6I CCWGG 3 cut(s) 670, 736, 2287
PspCI CACGTG 1 cut(s) 269
PspEI GGTNACC 1 cut(s) 3089
PspFI CCCAGC 2 cut(s) 1628, 1811
PspGI CCWGG 3 cut(s) 670, 736, 2287
PspN4I GGNNCC 8 cut(s) 51, 102, 1438, 1439, 1979, 2357, 2573, 3262
PspPI GGNCC 4 cut(s) 556, 1437, 1978, 2903
PspPPI RGGWCCY 1 cut(s) 1437
PstI CTGCAG 2 cut(s) 2077, 3624
PstNI CAGNNNCTG 5 cut(s) 307, 1433, 2072, 2415, 2605
PsuI RGATCY 5 cut(s) 49, 166, 871, 1542, 2993
PsyI GACNNNGTC 2 cut(s) 1435, 2177
PvuII CAGCTG 2 cut(s) 1676, 1811
RsaI GTAC 6 cut(s) 517, 958, 1944, 2813, 3144, 3600
RsaNI GTAC 6 cut(s) 516, 957, 1943, 2812, 3143, 3599
RseI CAYNNNNRTG 4 cut(s) 74, 594, 854, 1824
SacI GAGCTC 1 cut(s) 3811
SapI GCTCTTC 1 cut(s) 1041
SatI GCNGC 7 cut(s) 411, 1481, 1515, 1865, 3357, 3620, 3784
Sau96I GGNCC 4 cut(s) 556, 1437, 1978, 2903
ScaI AGTACT 3 cut(s) 517, 958, 3600
SchI GAGTC 5 cut(s) 341, 911, 1399, 1418, 1825
ScrFI CCNGG 3 cut(s) 672, 738, 2289
SduI GDGCHC 6 cut(s) 889, 1099, 2728, 3702, 3811, 3832
SexAI ACCWGGT 1 cut(s) 2287
SfcI CTRYAG 4 cut(s) 1638, 2073, 2307, 3620
SinI GGWCC 2 cut(s) 1437, 2903
SmiMI CAYNNNNRTG 4 cut(s) 74, 594, 854, 1824
SmlI CTYRAG 3 cut(s) 95, 147, 1928
SmoI CTYRAG 3 cut(s) 95, 147, 1928
SpeI ACTAGT 1 cut(s) 959
SphI GCATGC 3 cut(s) 593, 1364, 3397
SseBI AGGCCT 3 cut(s) 741, 1171, 3003
SsiI CCGC 5 cut(s) 118, 575, 1402, 1480, 3566
SspI AATATT 1 cut(s) 551
SspMI CTAG 5 cut(s) 489, 960, 1412, 1773, 3853
SstI GAGCTC 1 cut(s) 3811
StuI AGGCCT 3 cut(s) 741, 1171, 3003
StyD4I CCNGG 3 cut(s) 670, 736, 2287
StyI CCWWGG 1 cut(s) 2844
TaiI ACGT 4 cut(s) 271, 1299, 2879, 3061
TaqI TCGA 4 cut(s) 966, 1244, 1960, 2121
TatI WGTACW 5 cut(s) 515, 956, 1942, 2811, 3598
TauI GCSGC 1 cut(s) 1483
TscAI CASTG 4 cut(s) 227, 408, 1660, 2733
TseFI GTSAC 5 cut(s) 124, 264, 1421, 2338, 3640
TseI GCWGC 6 cut(s) 410, 1514, 1864, 3356, 3619, 3783
Tsp45I GTSAC 5 cut(s) 124, 264, 1421, 2338, 3640
TspGWI ACGGA 1 cut(s) 2564
TspRI CASTG 4 cut(s) 227, 408, 1660, 2733
Tth111I GACNNNGTC 2 cut(s) 1435, 2177
Vha464I CTTAAG 1 cut(s) 1928
VneI GTGCAC 1 cut(s) 2724
VpaK11BI GGWCC 2 cut(s) 1437, 2903
XapI RAATTY 7 cut(s) 569, 611, 1487, 1903, 2881, 3350, 3402
XceI RCATGY 5 cut(s) 593, 1364, 2534, 3397, 3712
XmiI GTMKAC 4 cut(s) 66, 398, 1756, 2668
XmnI GAANNNNTTC 3 cut(s) 1591, 3025, 3109
XspI CTAG 5 cut(s) 489, 960, 1412, 1773, 3853
ZrmI AGTACT 3 cut(s) 517, 958, 3600
Zsp2I ATGCAT 4 cut(s) 591, 1327, 2959, 3239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.