RchiOBHm_Chr2g0096511

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007281
Physical Location & Seq
Reverse (-)
9262658 .. 9274540
11883 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47143

Sequence Viewer

Length: 5271 bp
ATGGCGGGAGGCGCAGCCGGCGGCTTCGTGACGAGAGCCTATGAGTCCATGCTCAAAGAGTGCTCACCGAAAAAGTATTCCGATCTTCAGAAGGCAATTCAGGCTTATTTAGACAATGTGAAAGCGGTTAATCAGGCTGAACAAACCACACCACAAACCGCTCCAAGTGAGAAGAATCCAGCTACAGATGGTGATGGAAGTTCTCTTGAAACAGAAGGTGAAGCTCCAAAAACTGATGCAGAACCAGGTCAGTCTCAAACATCCTCACACACTGCCGAGGAGGCAGATTCTGTTGGCAGGCCAGTGAGCATAAGTGGAACTGTTTCAACTGTCCTAGCAACTGCTGGGAACACCTTAGAAGGCGCTCAGGCGGAGCTTGTTTTGAATCCCCTGCGACTTGCATTTGATACAAAGAACTTGAAAGTTCTAGAACCTGCTCTGGATTGTTTTCATAAACTCATTGCGTACGATCATTTAGAGGGAGATCCTGGTTTAGACGATAAAAGTGTTCCGGTGTTTACCGACATTTTGAACAGGGTTTGCAGTTGTGTTGACAATAATTCACCTGATAGTACTGTTCTGCAAGTGTTGAAGGTCCTTCTTACTGCAGTGGCATCCACAAAGTTTAGAGTACATGGGGAACCTTTGCTGGGAGTTATCAGAGTGTGCTATAATATTGCTCTGCACAGCAAGAGTCCTGTCAACCAAGCTACATCAAAGGCAATGCTTACGCAGATGATCAGCATCATATTCAGGAGAATGGAGACCGATCCGGAACTTCAGGTTTCATCATCTGGTTCTGTTGGACAAAATACAAATACAGCACAAAATTCAAATACAGAAGCTGAAGAAACTTCTGTGGCAGACCAAAATGAGAAAGGAATGACTTTAGGAGATCAACTTAACCAGGTTAAAGAGACACCCATTGCATCTGTTGAGGAACTTCATAATCTTGCAGGTGGTGCTGACATCAAGGGTTTAGAGGCCGTCCTGGACAAAGCCGTGCATCATGAAGATGGCAAAAAGATAACCAGAGGGATTGACCTGGAGAGTATGAGCATAGTTCAACGTGATGCTTTACTGGTTTTCCGAACCCTGTGCAAGATGGGCATGAAGGAAGATAACAATGAAGTTACGTTGAAGACGAGGATTTTATCTCTTGAGCTTCTGCAGGGTTTGTTGGAAGGAGTTGGTCATCCATTTACTAGGAACTTCCATTTTATTGATTCCGTGAAAGCATACCTATCATATGCTTTGTTGCGGGCTTCAGTTTCACCGTCCCCTATCATATTTCAGTATGCAAGTGGAATTTTTTTAGTGCTTTTGTTGCGGTTTAGAGAGAGTCTCAAGGGTGAAATAGGCATCTTCTTTCCCTTGATAGTTCTCCGGTCCCTGGATGGCTCGGATTTACCCATGAACCAGAAAATGAGTGTTCTTAGGATGTTAGAAAAAGTTTGCAAGGATCCTCAGATGCTTGTTGACATATTTGTGAACTATGACTGTGATCTTGAGGCACCGAACTTGTTTGAGCGGATGGTTACAACTCTATCCAGAATATCTCAAGGGACACAAAATGCAGATCCGAATATGGCTGGTGCATCCCAGACTACTTCAATAAAGGGTTCATCTCTTCAGTGTCTTGTGAATGTGCTTAAATCACTCGTAGATTGGGAGAAGTCACGTGGAGAATCAGATAGCCATAGTAAAAACACTCGGTCTCTGGAAGGAGATGCTTCAGCTAAAGAGTCTGTTGATGTGAAAAGCCGGCAAGATACAACCACTAACTTTGAGAAGGCAAAAGCTCATAAGTCTACACTGGAAGCTGCCATATCTGAGTTCAACAGGCGACCAGTGAAGGGTGTAGAGTATCTGAGATCAAATAAATTAGTGGAAAACACACCTAGTTCAGTTGCCCAATTTTTGAAAAGTACTCCCAGTTTGGACAAGGCTATGATTGGGGAATATTTGGGCCATCATGAAGAATTTCCTCTTTCTGTTATGCATGCTTATGTAGATTCCATGAAATTTTCGGGAATGAAGTTTGATACTGCAATTCGTGAACTTCTTAAAGGATTCCGACTTCCAGGGGAAGCTCAAAAGATAGATCGCATCATGGAAAAGTTTGCAGAAAGATACTGTGCAGATAATCCAGGCCTCTTCAAAAATGCAGATACTGCTTACGTTCTTGCATATGCAGTTATTATGTTGAATACTGATGCGCATAATCCAATGGTGTGGCCTAAGATGTCCAAGTCAGATTTCGTGCGCATGAATGCCACTGAAGATCCAGAAGATTGTGCTCCAACTGAACTTCTGGAAGAAATCTATGACTCAATTATTAAAGAAGAGATAAAGATGAAAGATGAGAGTACTAGTCTCGAGAAAAGTGGCAAATATAAGCCAGAAGGTGAAGAGAGAGGACGACTTGTCAGTATTCTTAATCTGGCTCTTCCCAGAAGGACACTGTCTTCTGATACCAAGTCTGAAAGTGAAGCTATCATAAAGAGAGCACAAGATATATTTAGGAATCAAGGAGCACAAAGAGGGGTCTTCCATACTACACAGCAGATAGAACTTGTAAGGCCTATGGTAGAAGCTGTTGGATGGCCTTTGCTTGCTACCTTCTCTGTTACAATGGAGGAGGGTGAGAATAAGTCGAGGGTTGTTCTCTGCATGGAAGGATTTAAAGCAGGAATACACATTACACATGTTCTGGGGATGGATACCATGCGCTATGCCTTCTTAACATCTCTGGTCAGATTTACATTCTTGCATGCTCCAAAGGAAATGCGTAGTAAAAATGTGGAAGCATTGCGAACTCTACTAGCTCTTTGTGACTCAGAGACTGGGTCCCTTCAAGACACATGGAATGCAGTTTTGGAATGTGTTTCACGGCTTGAATACATCACTTCAACCCCTGGTATTGCTGCAACAGTCATGCAGGGATCAAACCAAATCTCTAAAGATGCTGTTCTTCAATCTCTAAGAGAATTGGCTGGAAAACCTTCTGAGCAAGTATTTGTGAATAGTGTTCAGCTTCCCAGTGATTCTGTAGTGGAGTTTTTCACTGCACTCTGTGGTGTATCAGCGGAAGAACTAAAACAAAGTCCAGCTCGGGTTTTTAGCTTGCAGAAACTTGTTGAGATCAGCTATTACAACATGGCTCGTATACGCATGGTCTGGGCTAGGATATGGTCTGTTTTGGCAAACCACTTTATTTCAGCTGGGAGTCATCATGATGAGAAAATTGCTATGTATGCCATAGATTCTCTAAGGCAACTTGGTATGAAGTATCTGGAGCGAGCTGAACTTGCCAATTTCACATTCCAAAATGACATTCTTAAGCCTTTTGTTGTTCTTATGCGCAATAGTCGAAGTGAAACCATAAGGGGCCTAATTGTTGACTGCATTGTTCAAATGATAAAATCTAAAGTGGGGAGCATCAAGTCTGGTTGGCGTAGTGTTTTCATGATTTTTACAGCTTCTGCTGATGATGAATTGGAATCTATTGTTGAAAGTGCATTTGAAAATGTCGAACAGGTGATCTTGGAACACTTTGATCAAGTTGTTGGAGATTGTTTTATGGACTGTGTCAACTGCCTTATTAGATTTGCAAATAATAGAACTTCACACCGTATAAGTTTGAAGGCTATTGCACTCCTGCGCATATGTGAGGATCGTTTGGCTGAGGGTCTTATACCTGGTGGTGCTTTGAAGCCTATAGAAGAAAATGAGACTACAACTTTTGATGTGACCGAGCATTATTGGTTCCCAATGCTGGCTGGTCTGTCTGATCTGACATCAGATACAAGACCAGAAGTCAGAAGCTGTGCGCTTGAAGTTTTGTTTGATCTACTGAATGAGAGAGGTACCAAGTTCTCGTCATCCTTTTGGGAGAGCATCTTCCATCGTGTCTTGTTTCCCATATTTGATCATGTAAGAAATGCTGGAAAGGAAAGCTCGACTTCTTCTGATGAGGAATGGTTCCGTGAAACAAGCATTCACTCACTTCAGTTGCTGTGCAACCTTTTCAACACTTTTTATAAGGAGGTATGTTTTATGTTGCCACCACTACTCAGTCTACTGTTAGATTGTGCTAAACAGACTGATCAAGCAGTGGTTTCGTTATCTCTGGGTGCACTGGTTCATCTCATTGAAGTTGGAGGACATCAATTTAGTGAGAGTGATTGGGATACGTTATTGAAAAGCATAAGAGATGCTTTGTACACAACACAACCACTTGAACTGCTAAATGCTTTGGGTTTTGAGAACCTGAAGAATGATAGGACATTAAATGTAGAGGTCAATACCGGTGATGGTGCTTCCTTAATGCCTGTTGACTATGATGGGGGGGACAACCGTCAGTTTGATGCCAATGATAGAAATCCAAATGCATCAGTCGAATCAGGGGCGCAAATGAACTTGGATGGATCTGAAGGCCTCAACTCACCATCAGGATCTGCTTCAAAATCTGCTGAAGATGGAAACCTCCAACGTAGTCAAACAATAGGTCAAAGAATTATGGATAACCTCTTTCTCAGAAATCTTTCATCTAAGCCTAAGAGTTCTGATGCTTCAGTACCATCTACTCCAGTTAAGGTTCCTGATGCTGTCGAGCCTGATACCAAAGATGAGGAGGAAAGCTCTGTATTGGGAACTTGTAGGGGCAAATGCATTACTCAATTATTACTTCTGGGGGCCATTGATAGTATCCAGAAGAAATACTGGAGCAAGTTGAAGGCACCACAGAAGATTGCTATAATGGACATTTTGCTGTCCGCGTTAGAATTTGCAGCTTCCTACAATTCATATACAAATCTCAGGACACGCATGCACCAAATTTCTGATGAGAGGCCACCTCTGAATCTTCTTCGCCAAGAGTTAACAGGAACTTGCATATATCTGGACATCTTACAGAAAGCAACTTCCGGGTTTAGTGCCAATCAAGAGGGAAATGATGAAGAGAAAGTTGAAGGATTGGCAGAAGACAAACTGGTGTCTTTCTGTGAACAGGTACTCAGGGAGGCATCCGAGCTGCAGTCCAGTTCTGGAGATGTGACTAATATGGATATTCACCGTGTTCTGGAGTTACGATCTCCAATAATTGTTAAGGTGCTCAAAGGCATGTGCTTTATGAACCAACAGGTTTTCAGAAGACATCTGAGAGACTTCTATCCTTTGCTTACAAAACTTGTATGCTGCGACCAGATGGATGTTCGTGGAGCTCTTGGTGATCTATTTAGGGCACAATTGAAAGCACTTCTGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

1756

Amino Acids

194.68

Weight (kDa)

5.3

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCB PF16213 124 - 257 8.2e-26 Mon2/Sec7/BIG1-like, dimerisation and cyclophilin-binding domain
Sec7-like_HUS PF12783 356 - 508 7e-41 Mon2/Sec7/BIG1-like, HUS domain
Sec7 PF01369 603 - 784 2.2e-71 Sec7 domain
Sec7-like_HDS PF09324 1097 - 1179 2.1e-26 Mon2/Sec7/BIG1-like, HDS
Mon2_C PF16206 1253 - 1327 3.8e-08 C-terminal region of Mon2 protein
BIG2_C PF20252 1560 - 1747 4.2e-28 BIG2 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4044
AarI CACCTGC 1 cut(s) 947
Acc16I TGCGCA 4 cut(s) 2220, 2267, 3365, 3665
Acc36I ACCTGC 2 cut(s) 442, 947
Acc65I GGTACC 1 cut(s) 3869
AccB1I GGYRCC 3 cut(s) 1513, 3869, 4741
AccBSI CCGCTC 2 cut(s) 161, 1531
AccI GTMKAC 3 cut(s) 1811, 3169, 4081
AccII CGCG 1 cut(s) 4781
AccIII TCCGGA 1 cut(s) 772
AcsI RAATTY 6 cut(s) 829, 1308, 1982, 2024, 4787, 4839
AcvI CACGTG 1 cut(s) 1682
AdeI CACNNNGTG 1 cut(s) 3077
AfaI GTAC 9 cut(s) 467, 574, 633, 1930, 2371, 3871, 4226, 4581, 5016
AfiI CCNNNNNNNGG 5 cut(s) 650, 1393, 1856, 3778, 5083
AflII CTTAAG 1 cut(s) 3341
AflIII ACRYGT 1 cut(s) 2707
AgeI ACCGGT 1 cut(s) 4310
AhdI GACNNNNNGTC 2 cut(s) 2426, 3818
AhlI ACTAGT 1 cut(s) 2372
AjuI GAANNNNNNNTTGG 8 cut(s) 2243, 2275, 2861, 2893, 3308, 3321, 3340, 3353
Alw21I GWGCWC 7 cut(s) 65, 2302, 2512, 2539, 4141, 5118, 5227
Alw26I GTCTC 9 cut(s) 258, 758, 911, 1349, 1722, 2381, 2837, 3728, 5160
Alw44I GTGCAC 1 cut(s) 4137
AlwNI CAGNNNCTG 8 cut(s) 290, 845, 2174, 2846, 3485, 3828, 4018, 4460
Ama87I CYCGRG 2 cut(s) 2378, 3114
Aor13HI TCCGGA 1 cut(s) 772
ApaLI GTGCAC 1 cut(s) 4137
ApeKI GCWGC 6 cut(s) 14, 1823, 2927, 4793, 5035, 5199
ApoI RAATTY 6 cut(s) 829, 1308, 1982, 2024, 4787, 4839
ArsI GACNNNNNNTTYG 2 cut(s) 4495, 4527
AsiGI ACCGGT 1 cut(s) 4310
Asp700I GAANNNNTTC 4 cut(s) 322, 1983, 3004, 4546
Asp718I GGTACC 1 cut(s) 3869
AspLEI GCGC 9 cut(s) 14, 365, 2221, 2268, 2733, 3366, 3666, 3835, 4414
AspS9I GGNCC 6 cut(s) 595, 1389, 1969, 2850, 3391, 4698
AsuC2I CCSGG 1 cut(s) 4930
AvaI CYCGRG 2 cut(s) 2378, 3114
AvaII GGWCC 3 cut(s) 595, 1389, 2850
BaeGI GKGCMC 2 cut(s) 4141, 5248
BamHI GGATCC 1 cut(s) 1462
BanI GGYRCC 3 cut(s) 1513, 3869, 4741
BanII GRGCYC 1 cut(s) 5227
BbrPI CACGTG 1 cut(s) 1682
BbsI GAAGAC 5 cut(s) 1148, 2460, 2542, 4992, 5161
Bbv12I GWGCWC 7 cut(s) 65, 2302, 2512, 2539, 4141, 5118, 5227
BbvCI CCTCAGC 1 cut(s) 3687
BbvI GCAGC 6 cut(s) 26, 1810, 2914, 4805, 5022, 5186
BceAI ACGGC 4 cut(s) 971, 986, 2909, 5251
BcgI CGANNNNNNTGC 4 cut(s) 1080, 1114, 4104, 4138
BciVI GTATCC 3 cut(s) 2716, 4186, 4721
BclI TGATCA 4 cut(s) 738, 3559, 3931, 4108
BcnI CCSGG 1 cut(s) 4930
BcoDI GTCTC 9 cut(s) 258, 758, 911, 1349, 1722, 2381, 2837, 3728, 5160
BcuI ACTAGT 1 cut(s) 2372
BfaI CTAG 7 cut(s) 335, 428, 1206, 1902, 2373, 2825, 3186
BfmI CTRYAG 6 cut(s) 183, 606, 1169, 3051, 3720, 5036
BfoI RGCGCY 1 cut(s) 366
BfrI CTTAAG 1 cut(s) 3341
BfuAI ACCTGC 2 cut(s) 442, 947
BfuI GTATCC 3 cut(s) 2716, 4186, 4721
BglI GCCNNNNNGGC 1 cut(s) 281
BisI GCNGC 7 cut(s) 15, 22, 1824, 2928, 4794, 5036, 5200
BlsI GCNGC 7 cut(s) 16, 23, 1825, 2929, 4795, 5037, 5201
BmcAI AGTACT 3 cut(s) 574, 1930, 2371
Bme18I GGWCC 3 cut(s) 595, 1389, 2850
BmeRI GACNNNNNGTC 2 cut(s) 2426, 3818
BmeT110I CYCGRG 2 cut(s) 2378, 3114
BmgT120I GGNCC 6 cut(s) 595, 1389, 1969, 2850, 3391, 4698
BmrI ACTGGG 3 cut(s) 1929, 2856, 3036
BmuI ACTGGG 3 cut(s) 1929, 2856, 3036
BoxI GACNNNNGTC 1 cut(s) 4359
BpiI GAAGAC 5 cut(s) 1148, 2460, 2542, 4992, 5161
BplI GAGNNNNNCTC 6 cut(s) 1329, 1361, 4628, 4660, 4843, 4875
BpmI CTGGAG 6 cut(s) 1067, 3317, 4575, 4747, 5070, 5105
Bpu10I CCTNAGC 2 cut(s) 366, 3687
BpuEI CTTGAG 4 cut(s) 1181, 1331, 1529, 1545
BpuMI CCSGG 1 cut(s) 4930
BsaAI YACGTR 1 cut(s) 1682
BsaBI GATNNNNATC 2 cut(s) 743, 4383
BsaI GGTCTC 2 cut(s) 758, 1722
BsaJI CCNNGG 4 cut(s) 276, 1392, 2084, 2917
BsaWI WCCGGW 4 cut(s) 511, 772, 1386, 4310
Bsc4I CCNNNNNNNGG 5 cut(s) 650, 1393, 1856, 3778, 5083
Bse118I RCCGGY 3 cut(s) 17, 1764, 4310
Bse3DI GCAATG 4 cut(s) 459, 729, 924, 2810
Bse8I GATNNNNATC 2 cut(s) 743, 4383
BseAI TCCGGA 1 cut(s) 772
BseDI CCNNGG 4 cut(s) 276, 1392, 2084, 2917
BseJI GATNNNNATC 2 cut(s) 743, 4383
BseLI CCNNNNNNNGG 5 cut(s) 650, 1393, 1856, 3778, 5083
BseMI GCAATG 4 cut(s) 459, 729, 924, 2810
BseRI GAGGAG 3 cut(s) 293, 2654, 4649
BseSI GKGCMC 2 cut(s) 4141, 5248
BseXI GCAGC 6 cut(s) 26, 1810, 2914, 4805, 5022, 5186
BseYI CCCAGC 3 cut(s) 344, 649, 3224
BsgI GTGCAG 3 cut(s) 668, 2160, 3054
Bsh1236I CGCG 1 cut(s) 4781
BshNI GGYRCC 3 cut(s) 1513, 3869, 4741
BshTI ACCGGT 1 cut(s) 4310
BsiHKAI GWGCWC 7 cut(s) 65, 2302, 2512, 2539, 4141, 5118, 5227
BsiHKCI CYCGRG 2 cut(s) 2378, 3114
BsiSI CCGG 7 cut(s) 18, 512, 773, 1387, 1765, 4311, 4929
BsiWI CGTACG 1 cut(s) 465
BslFI GGGAC 5 cut(s) 1264, 1375, 1579, 2836, 4367
BslI CCNNNNNNNGG 5 cut(s) 650, 1393, 1856, 3778, 5083
BsmAI GTCTC 9 cut(s) 258, 758, 911, 1349, 1722, 2381, 2837, 3728, 5160
BsmFI GGGAC 5 cut(s) 1264, 1375, 1579, 2836, 4367
BsmI GAATGC 3 cut(s) 2278, 2875, 3999
Bso31I GGTCTC 2 cut(s) 758, 1722
BsoBI CYCGRG 2 cut(s) 2378, 3114
Bsp1286I GDGCHC 8 cut(s) 65, 2302, 2512, 2539, 4141, 5118, 5227, 5248
Bsp13I TCCGGA 1 cut(s) 772
Bsp1407I TGTACA 1 cut(s) 4224
BspEI TCCGGA 1 cut(s) 772
BspFNI CGCG 1 cut(s) 4781
BspHI TCATGA 4 cut(s) 1009, 1975, 3235, 3468
BspMAI CTGCAG 3 cut(s) 610, 1173, 5040
BspMI ACCTGC 2 cut(s) 442, 947
BspQI GCTCTTC 1 cut(s) 2454
BspT107I GGYRCC 3 cut(s) 1513, 3869, 4741
BspTI CTTAAG 1 cut(s) 3341
BspTNI GGTCTC 2 cut(s) 758, 1722
BsrBI CCGCTC 2 cut(s) 161, 1531
BsrDI GCAATG 4 cut(s) 459, 729, 924, 2810
BsrFI RCCGGY 3 cut(s) 17, 1764, 4310
BsrGI TGTACA 1 cut(s) 4224
BssAI RCCGGY 3 cut(s) 17, 1764, 4310
BssECI CCNNGG 4 cut(s) 276, 1392, 2084, 2917
BssNAI GTATAC 1 cut(s) 3170
Bst1107I GTATAC 1 cut(s) 3170
Bst6I CTCTTC 6 cut(s) 1635, 2162, 2340, 2406, 2454, 4956
BstAFI CTTAAG 1 cut(s) 3341
BstAPI GCANNNNNTGC 2 cut(s) 962, 2174
BstAUI TGTACA 1 cut(s) 4224
BstBAI YACGTR 1 cut(s) 1682
BstFNI CGCG 1 cut(s) 4781
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 9 cut(s) 14, 365, 2221, 2268, 2733, 3366, 3666, 3835, 4414
BstMAI GTCTC 9 cut(s) 258, 758, 911, 1349, 1722, 2381, 2837, 3728, 5160
BstNSI RCATGY 5 cut(s) 2006, 2711, 2777, 4834, 5128
BstPAI GACNNNNGTC 1 cut(s) 4359
BstSFI CTRYAG 6 cut(s) 183, 606, 1169, 3051, 3720, 5036
BstSLI GKGCMC 2 cut(s) 4141, 5248
BstUI CGCG 1 cut(s) 4781
BstV1I GCAGC 6 cut(s) 26, 1810, 2914, 4805, 5022, 5186
BstV2I GAAGAC 5 cut(s) 1148, 2460, 2542, 4992, 5161
BstX2I RGATCY 6 cut(s) 484, 1462, 1579, 2284, 4430, 4457
BstXI CCANNNNNNTGG 1 cut(s) 2235
BstYI RGATCY 6 cut(s) 484, 1462, 1579, 2284, 4430, 4457
BstZ17I GTATAC 1 cut(s) 3170
BsuI GTATCC 3 cut(s) 2716, 4186, 4721
BtsI GCAGTG 4 cut(s) 270, 615, 3066, 4122
BveI ACCTGC 2 cut(s) 442, 947
CaiI CAGNNNCTG 8 cut(s) 290, 845, 2174, 2846, 3485, 3828, 4018, 4460
CciI TCATGA 4 cut(s) 1009, 1975, 3235, 3468
CfoI GCGC 9 cut(s) 14, 365, 2221, 2268, 2733, 3366, 3666, 3835, 4414
Cfr10I RCCGGY 3 cut(s) 17, 1764, 4310
Cfr13I GGNCC 6 cut(s) 595, 1389, 1969, 2850, 3391, 4698
CsiI ACCWGGT 3 cut(s) 244, 906, 3700
Csp6I GTAC 9 cut(s) 466, 573, 632, 1929, 2370, 3870, 4225, 4580, 5015
CspAI ACCGGT 1 cut(s) 4310
CspCI CAANNNNNGTGG 2 cut(s) 1768, 1803
CviQI GTAC 9 cut(s) 466, 573, 632, 1929, 2370, 3870, 4225, 4580, 5015
DraI TTTAAA 1 cut(s) 2686
DraIII CACNNNGTG 1 cut(s) 3077
DriI GACNNNNNGTC 2 cut(s) 2426, 3818
Eam1104I CTCTTC 6 cut(s) 1635, 2162, 2340, 2406, 2454, 4956
Eam1105I GACNNNNNGTC 2 cut(s) 2426, 3818
EarI CTCTTC 6 cut(s) 1635, 2162, 2340, 2406, 2454, 4956
EciI GGCGGA 1 cut(s) 386
Ecl136II GAGCTC 1 cut(s) 5225
Eco147I AGGCCT 3 cut(s) 2154, 2584, 4440
Eco24I GRGCYC 1 cut(s) 5227
Eco31I GGTCTC 2 cut(s) 758, 1722
Eco47I GGWCC 3 cut(s) 595, 1389, 2850
Eco53kI GAGCTC 1 cut(s) 5225
Eco72I CACGTG 1 cut(s) 1682
Eco88I CYCGRG 2 cut(s) 2378, 3114
EcoICRI GAGCTC 1 cut(s) 5225
EcoO109I RGGNCCY 3 cut(s) 595, 2850, 3391
EcoT22I ATGCAT 3 cut(s) 2004, 4396, 4676
EcoT38I GRGCYC 1 cut(s) 5227
FalI AAGNNNNNCTT 4 cut(s) 3949, 3981, 4204, 4236
FaqI GGGAC 5 cut(s) 1264, 1375, 1579, 2836, 4367
FauI CCCGC 1 cut(s) 1254
FauNDI CATATG 3 cut(s) 1249, 2191, 3668
FbaI TGATCA 4 cut(s) 738, 3559, 3931, 4108
FblI GTMKAC 3 cut(s) 1811, 3169, 4081
Fnu4HI GCNGC 7 cut(s) 15, 22, 1824, 2928, 4794, 5036, 5200
FriOI GRGCYC 1 cut(s) 5227
Fsp4HI GCNGC 7 cut(s) 15, 22, 1824, 2928, 4794, 5036, 5200
FspAI RTGCGCAY 2 cut(s) 2220, 2267
FspBI CTAG 7 cut(s) 335, 428, 1206, 1902, 2373, 2825, 3186
FspI TGCGCA 4 cut(s) 2220, 2267, 3365, 3665
GlaI GCGC 9 cut(s) 13, 364, 2220, 2267, 2732, 3365, 3665, 3834, 4413
GluI GCNGC 7 cut(s) 15, 22, 1824, 2928, 4794, 5036, 5200
GsaI CCCAGC 3 cut(s) 348, 653, 3228
GsuI CTGGAG 6 cut(s) 1067, 3317, 4575, 4747, 5070, 5105
HaeII RGCGCY 1 cut(s) 366
HapII CCGG 7 cut(s) 18, 512, 773, 1387, 1765, 4311, 4929
HhaI GCGC 9 cut(s) 14, 365, 2221, 2268, 2733, 3366, 3666, 3835, 4414
Hin6I GCGC 9 cut(s) 12, 363, 2219, 2266, 2731, 3364, 3664, 3833, 4412
HinP1I GCGC 9 cut(s) 12, 363, 2219, 2266, 2731, 3364, 3664, 3833, 4412
HincII GTYRAC 7 cut(s) 553, 703, 1480, 3403, 3595, 4339, 4884
HindII GTYRAC 7 cut(s) 553, 703, 1480, 3403, 3595, 4339, 4884
HpaI GTTAAC 1 cut(s) 4884
HpaII CCGG 7 cut(s) 18, 512, 773, 1387, 1765, 4311, 4929
HpyCH4IV ACGT 6 cut(s) 1069, 1136, 1681, 2181, 4196, 4495
HpySE526I ACGT 6 cut(s) 1069, 1136, 1681, 2181, 4196, 4495
HspAI GCGC 9 cut(s) 12, 363, 2219, 2266, 2731, 3364, 3664, 3833, 4412
KflI GGGWCCC 1 cut(s) 2850
Kpn2I TCCGGA 1 cut(s) 772
KpnI GGTACC 1 cut(s) 3873
KroI GCCGGC 2 cut(s) 17, 1764
KroNI GCCGGC 2 cut(s) 19, 1766
Ksp22I TGATCA 4 cut(s) 738, 3559, 3931, 4108
KspAI GTTAAC 1 cut(s) 4884
LguI GCTCTTC 1 cut(s) 2454
Lsp1109I GCAGC 6 cut(s) 26, 1810, 2914, 4805, 5022, 5186
MabI ACCWGGT 3 cut(s) 244, 906, 3700
MaeI CTAG 7 cut(s) 335, 428, 1206, 1902, 2373, 2825, 3186
MaeII ACGT 6 cut(s) 1069, 1136, 1681, 2181, 4196, 4495
MaeIII GTNAC 9 cut(s) 28, 1132, 1537, 1677, 2629, 2834, 3751, 5056, 5088
MbiI CCGCTC 2 cut(s) 161, 1531
MfeI CAATTG 1 cut(s) 5249
MflI RGATCY 6 cut(s) 484, 1462, 1579, 2284, 4430, 4457
MhlI GDGCHC 8 cut(s) 65, 2302, 2512, 2539, 4141, 5118, 5227, 5248
MlyI GAGTC 7 cut(s) 53, 703, 1351, 1754, 2324, 2831, 3238
MmeI TCCRAC 8 cut(s) 784, 1161, 2101, 2327, 2581, 3550, 4141, 4516
Mph1103I ATGCAT 3 cut(s) 2004, 4396, 4676
MroI TCCGGA 1 cut(s) 772
MroNI GCCGGC 2 cut(s) 17, 1764
MroXI GAANNNNTTC 4 cut(s) 322, 1983, 3004, 4546
MslI CAYNNNNRTG 4 cut(s) 1014, 1487, 2007, 3237
MspA1I CMGCKG 2 cut(s) 3089, 3224
MspCI CTTAAG 1 cut(s) 3341
MspI CCGG 7 cut(s) 18, 512, 773, 1387, 1765, 4311, 4929
MunI CAATTG 1 cut(s) 5249
Mva1269I GAATGC 3 cut(s) 2278, 2875, 3999
MvnI CGCG 1 cut(s) 4781
NaeI GCCGGC 2 cut(s) 19, 1766
NciI CCSGG 1 cut(s) 4930
NdeI CATATG 3 cut(s) 1249, 2191, 3668
NgoMIV GCCGGC 2 cut(s) 17, 1764
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 5 cut(s) 28, 1677, 2834, 3751, 5056
NsbI TGCGCA 4 cut(s) 2220, 2267, 3365, 3665
NsiI ATGCAT 3 cut(s) 2004, 4396, 4676
NspI RCATGY 5 cut(s) 2006, 2711, 2777, 4834, 5128
PaeI GCATGC 3 cut(s) 2006, 2777, 4834
PaeR7I CTCGAG 1 cut(s) 2378
PagI TCATGA 4 cut(s) 1009, 1975, 3235, 3468
PaqCI CACCTGC 1 cut(s) 947
PceI AGGCCT 3 cut(s) 2154, 2584, 4440
PciI ACATGT 1 cut(s) 2707
PciSI GCTCTTC 1 cut(s) 2454
PcsI WCGNNNNNNNCGW 1 cut(s) 1142
PctI GAATGC 3 cut(s) 2278, 2875, 3999
PdiI GCCGGC 2 cut(s) 19, 1766
PdmI GAANNNNTTC 4 cut(s) 322, 1983, 3004, 4546
Pfl23II CGTACG 1 cut(s) 465
PflFI GACNNNGTC 3 cut(s) 2464, 2848, 3590
PfoI TCCNGGA 1 cut(s) 990
PinAI ACCGGT 1 cut(s) 4310
PkrI GCNGC 7 cut(s) 16, 23, 1825, 2929, 4795, 5037, 5201
PleI GAGTC 7 cut(s) 52, 702, 1350, 1753, 2324, 2831, 3237
PmaCI CACGTG 1 cut(s) 1682
PmlI CACGTG 1 cut(s) 1682
PpsI GAGTC 7 cut(s) 52, 702, 1350, 1753, 2324, 2831, 3237
Ppu21I YACGTR 1 cut(s) 1682
PpuMI RGGWCCY 2 cut(s) 595, 2850
PscI ACATGT 1 cut(s) 2707
PshAI GACNNNNGTC 1 cut(s) 4359
PsiI TTATAA 1 cut(s) 4044
Psp124BI GAGCTC 1 cut(s) 5227
Psp5II RGGWCCY 2 cut(s) 595, 2850
PspCI CACGTG 1 cut(s) 1682
PspFI CCCAGC 3 cut(s) 344, 649, 3224
PspLI CGTACG 1 cut(s) 465
PspPI GGNCC 6 cut(s) 595, 1389, 1969, 2850, 3391, 4698
PspPPI RGGWCCY 2 cut(s) 595, 2850
PstI CTGCAG 3 cut(s) 610, 1173, 5040
PstNI CAGNNNCTG 8 cut(s) 290, 845, 2174, 2846, 3485, 3828, 4018, 4460
PsuI RGATCY 6 cut(s) 484, 1462, 1579, 2284, 4430, 4457
PsyI GACNNNGTC 3 cut(s) 2464, 2848, 3590
PvuII CAGCTG 1 cut(s) 3224
RsaI GTAC 9 cut(s) 467, 574, 633, 1930, 2371, 3871, 4226, 4581, 5016
RsaNI GTAC 9 cut(s) 466, 573, 632, 1929, 2370, 3870, 4225, 4580, 5015
RseI CAYNNNNRTG 4 cut(s) 1014, 1487, 2007, 3237
SacI GAGCTC 1 cut(s) 5227
SapI GCTCTTC 1 cut(s) 2454
SatI GCNGC 7 cut(s) 15, 22, 1824, 2928, 4794, 5036, 5200
Sau96I GGNCC 6 cut(s) 595, 1389, 1969, 2850, 3391, 4698
ScaI AGTACT 3 cut(s) 574, 1930, 2371
SchI GAGTC 7 cut(s) 53, 703, 1351, 1754, 2324, 2831, 3238
SduI GDGCHC 8 cut(s) 65, 2302, 2512, 2539, 4141, 5118, 5227, 5248
SexAI ACCWGGT 3 cut(s) 244, 906, 3700
SfcI CTRYAG 6 cut(s) 183, 606, 1169, 3051, 3720, 5036
Sfr274I CTCGAG 1 cut(s) 2378
SinI GGWCC 3 cut(s) 595, 1389, 2850
SlaI CTCGAG 1 cut(s) 2378
SmiMI CAYNNNNRTG 4 cut(s) 1014, 1487, 2007, 3237
SmlI CTYRAG 6 cut(s) 1160, 1346, 1508, 1560, 2378, 3341
SmoI CTYRAG 6 cut(s) 1160, 1346, 1508, 1560, 2378, 3341
SpeI ACTAGT 1 cut(s) 2372
SphI GCATGC 3 cut(s) 2006, 2777, 4834
SseBI AGGCCT 3 cut(s) 2154, 2584, 4440
SspI AATATT 2 cut(s) 676, 1964
SspMI CTAG 7 cut(s) 335, 428, 1206, 1902, 2373, 2825, 3186
SstI GAGCTC 1 cut(s) 5227
StuI AGGCCT 3 cut(s) 2154, 2584, 4440
TaiI ACGT 6 cut(s) 1072, 1139, 1684, 2184, 4199, 4498
TaqI TCGA 7 cut(s) 2379, 2657, 3373, 3534, 3962, 4401, 4614
TaqII GACCGA 3 cut(s) 782, 1704, 3770
TatI WGTACW 5 cut(s) 572, 631, 1928, 2369, 4224
TauI GCSGC 1 cut(s) 24
TseFI GTSAC 5 cut(s) 28, 1677, 2834, 3751, 5056
TseI GCWGC 6 cut(s) 14, 1823, 2927, 4793, 5035, 5199
Tsp45I GTSAC 5 cut(s) 28, 1677, 2834, 3751, 5056
TspGWI ACGGA 2 cut(s) 1219, 3977
Tth111I GACNNNGTC 3 cut(s) 2464, 2848, 3590
Vha464I CTTAAG 1 cut(s) 3341
VneI GTGCAC 1 cut(s) 4137
VpaK11BI GGWCC 3 cut(s) 595, 1389, 2850
XapI RAATTY 6 cut(s) 829, 1308, 1982, 2024, 4787, 4839
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 5 cut(s) 2006, 2711, 2777, 4834, 5128
XhoI CTCGAG 1 cut(s) 2378
XmiI GTMKAC 3 cut(s) 1811, 3169, 4081
XmnI GAANNNNTTC 4 cut(s) 322, 1983, 3004, 4546
XspI CTAG 7 cut(s) 335, 428, 1206, 1902, 2373, 2825, 3186
ZrmI AGTACT 3 cut(s) 574, 1930, 2371
Zsp2I ATGCAT 3 cut(s) 2004, 4396, 4676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.