AT3G49500
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008702
Physical Location & Seq
Reverse (-)
18348974 .. 18353673
4700 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G49500.1

Sequence Viewer

Length: 3591 bp
ATGGGGTCAGAGGGAAATATGAAGAAGTCGGTTGTGACACAAGTAAGCATTGGTGGATTTGGGGAATCCACTACTGCAAAACAACTCACAGATTACCTTGAAGATGAAGTAGGAATTGTTTGGCGTTGCAGATTAAAAACTTCGTGGACTCCTCCTGGGTCTTATCCTAATTTTGAAATCGCAGACACTTCAAATATCCCCAGTATTGATGAATATAAGAAAGTGGAGCCTCATGCCTTTGTCCATTTCGCAGTTTTTGAATCAGCTGGTCGTGCCATGGATGCTGCAGGGCAATGCAACCTCATCTTGGATGGCCAGCCGTTAAAAGTCAGTTTGGGTCCTAAGAATCCATATTCCCTTAATCAAAGAAGAAGAACGACTGTACCTTATAAGTTGGCTGGTATTACACTTGAGATTGGGACATTGGTTTCTCGGGATGATTTTTTTGTTTCTTGGAGAGCTGAAGGGGTTGATTTCCTGGTGGATCCTTTTGACAACACGTGCAAGTTTTGTTTCAGAAAGAGCACTGCCTTCTCTTTCAAGGATGCAGTGATGCATGCTGTGATCAATTGTGATTATAAGTTGGAGTTATTGGTGAGAGATATACAAACAGTCAGGCAGTATAAAACCTTGCATGGCTTTGTGCTTATTTTGCAGCTGGCTTCTTCACCCCGTGTCTGGTACAGAACTGCTGATGATGATATTTACGATACTGTCCCTGGCGATCTCTTGGATGATGATGATCCTTGGATCCGTACCACTGATTTTACTCAAGTTGGGGCAATTGGTCGATGTCATTCATATCGAGTGCTCATATCTCCGCGGTATGAAAATAAACTGAGAACAGCCTTAGATTATTTTAGGATGCGGAGGGTGCAAGAGGAACGTGTGAGGTGGCCTCCCCGTATCCGTAATGAGCCTTGTTTTGGGGAGCCTGTGTCAGATCATTTCTTCTGCATTCATCACAAGGAAGGAATCTCTTTTGAGATCATGTTTCTAGTAAATTCGGTATTGCACAGGGGTGTCTTTAACCAGTTTCAGTTGACTGAGCGATTCTTTGATCTTCTAAGAAACCAACCCAAGGACGTCAATATAGCTTCTCTCAAGCATCTCTGTACCTATAAACGACCAGTTTTTGATGCGTACAAGAGGTTGAAGCTTGTTCAGGAATGGATTCAGAAAAATCCAAAGCTTTTAGGGAGTCATGAACAATCTGAGGATATCTCTGAGATCAGAAGACTAGTAATTACCCCAACCAGAGCCTATTGCCTACCCCCAGAAGTTGAGCTCTCCAACAGGGTACTCAGGAGATACAAAGCTGTTGCTGAAAGATTTTTGCGGGTAACTTTCATGGATGAAAGTATGCAGACCATAAATTCGAATGTTCTCTCTTACTTTGTTGCTCCTATTGTGAAGGATTTGACATCAAGTTCTTTCTCCCAGAAGACCTACGTTTTTAAAAGAGTGAAGAGCATATTAACCGATGGGTTTAAACTATGTGGTAGAAAATACAGTTTTCTAGCATTCTCAGCCAATCAACTGAGAGACCGCTCTGCATGGTTCTTTGCTGAAGACGGGAAAACACGTGTGTCAGATATAAAAACATGGATGGGGAAGTTCAAAGACAAGAATGTGGCAAAATGTGCTGCTAGGATGGGCCTGTGCTTCTCCTCCACATATGCCACTGTAGATGTCATGCCTCACGAGGTTGACACTGAGGTTCCAGATATTGAGAGAAATGGGTATGTTTTCTCTGACGGAATTGGTACAATCACACCTGACCTCGCTGACGAAGTAATGGAGAAACTTAAGTTGGATGTGCACTACAGCCCTTGTGCTTATCAGATACGTTACGCAGGTTTCAAAGGGGTTGTTGCTCGTTGGCCATCAAAAAGTGATGGAATCAGGCTAGCCCTTCGAGACAGTATGAAGAAGTTCTTTTCCAAACATACGATCTTGGAGATCTGTTCCTGGACGAGGTTTCAACCTGGGTTCTTAAATCGGCAGATAATTACCCTTCTATCCGTACTAGGTGTTCCGGATGAAATATTCTGGGATATGCAGGAATCCATGCTCTATAAACTGAACCGCATCCTTGATGATACAGATGTGGCATTTGAAGTTCTCACGGCATCATGTGCTGAACAGGGAAACACTGCAGCTATCATGCTTAGTGCAGGTTTCAAACCAAAAACCGAGCCGCATCTACGCGGGATGTTGTCTTCAGTCAGAATTGCACAACTCTGGGGTCTCAGAGAAAAATCTCGTATTTTTGTTACTTCAGGAAGGTGGCTAATGGGTTGCCTAGACGAAGCAGGGATACTTGAACATGGCCAATGCTTTATTCAAGTCTCTAAACCGTCTATAGAAAATTGTTTCTCCAAACATGGGTCTCGTTTTAAGGAGACAAAGACAGATCTGGAAGTAGTTAAAGGCTATGTAGCCATTGCTAAGAATCCTTGTCTTCACCCAGGGGATGTAAGGATTTTAGAAGCTGTTGATGTACCCCAGCTGCATCACATGTATGACTGCCTTATTTTCCCTCAGAAAGGTGATAGGCCGCATACAAACGAAGCTTCTGGCAGTGACCTTGACGGGGACCTGTACTTTGTGGCTTGGGATCAGAAACTCATCCCTCCCAACAGGAAAAGCTATCCGGCCATGCATTATGATGCAGCTGAAGAGAAGAGTTTAGGCCGTGCTGTCAACCACCAGGACATAATCGATTTCTTTGCAAGAAACTTGGCGAATGAGCAGTTGGGCACAATTTGCAATGCACACGTCGTTCATGCTGATAGAAGTGAGTATGGAGCCATGGACGAAGAATGTTTGCTACTGGCAGAACTAGCTGCCACTGCAGTTGATTTCCCAAAGACAGGGAAAATTGTGTCAATGCCCTTCCACCTAAAACCAAAACTCTACCCAGATTTCATGGGAAAAGAAGACTACCAAACTTACAAGTCGAACAAAATCTTGGGTCGGCTTTACAGACGGGTAAAAGAGGTTTATGATGAAGATGCAGAAGCTTCCTCAGAAGAAAGCACAGACCCAAGTGCCATCCCTTATGACGCTGTTCTTGAAATACCGGGATTTGAAGATTTGATCCCTGAGGCATGGGGTCACAAATGTTTGTACGACGGGCAACTCATTGGTCTTCTTGGGCAATACAAGGTGCAGAAAGAGGAAGAGATTGTGACGGGTCACATCTGGTCCATGCCCAAATACACAAGCAAGAAACAAGGCGAACTGAAAGAAAGACTGAAGCATTCTTATAATTCCCTTAAAAAGGAGTTCAGGAAAGTATTTGAGGAAACAATCCCTGACCATGAAAATCTCAGCGAGGAGGAAAAAAACATCTTGTATGAGAAGAAGGCTTCAGCTTGGTATCATGTAACTTACCATCCCGAGTGGGTGAAGAAGTCTTTAGAGCTGCAAGATCCAGATGAGTCCAGTCATGCGGCGATGCTGAGTTTTGCTTGGATTGCAGCTGATTATCTTGCAAGAATCAAAATCCGGTCACGGGAAATGGGAAGTATCGACTCAGCCAAGCCTGTTGATTCTTTGGCTAAGTTTCTTGCTCAGCGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1196

Amino Acids

136.93

Weight (kDa)

6.82

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_RDR6 PF24572 8 - 111 1.8e-35 RNA-dependent RNA polymerase 6, RNA-binding domain
RDR6_2nd PF24577 131 - 292 7.3e-76 RNA-dependent RNA polymerase 6 second domain
PH_RDR2 PF24823 137 - 274 3.2e-09 RDR2 PH-like domain
RdRP_helical PF26252 315 - 400 7.7e-30 RNA dependent RNA polymerase, helical domain
RdRP PF05183 416 - 999 3.7e-190 RNA dependent RNA polymerase
RdRP_head PF26253 1021 - 1190 1.3e-50 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 390, 579, 3273
AatII GACGTC 1 cut(s) 1089
Acc36I ACCTGC 2 cut(s) 1847, 2168
AccBSI CCGCTC 1 cut(s) 1551
AccII CGCG 2 cut(s) 823, 2211
AccIII TCCGGA 1 cut(s) 2038
AclWI GGATC 8 cut(s) 479, 492, 737, 745, 758, 2628, 3097, 3431
AcoI YGGCCR 4 cut(s) 313, 1883, 2332, 2658
AcsI RAATTY 2 cut(s) 1003, 1375
AcuI CTGAAG 7 cut(s) 483, 1590, 2208, 2265, 2700, 3281, 3360
AcvI CACGTG 2 cut(s) 501, 1586
AcyI GRCGYC 1 cut(s) 1086
AdeI CACNNNGTG 1 cut(s) 674
AflII CTTAAG 1 cut(s) 1808
AflIII ACRYGT 6 cut(s) 498, 886, 1583, 1585, 2520, 2779
AhlI ACTAGT 1 cut(s) 1240
AjiI CACGTC 1 cut(s) 2782
AjnI CCWGG 7 cut(s) 154, 477, 718, 1970, 1987, 2470, 2712
AjuI GAANNNNNNNTTGG 8 cut(s) 1796, 1828, 2328, 2360, 2741, 2773, 2957, 2989
AleI CACNNNNGTG 1 cut(s) 1020
Alw21I GWGCWC 4 cut(s) 527, 813, 1290, 1824
Alw26I GTCTC 6 cut(s) 1539, 1914, 2255, 2356, 2397, 2399
Alw44I GTGCAC 1 cut(s) 1820
AlwI GGATC 8 cut(s) 479, 492, 737, 745, 758, 2628, 3097, 3431
Ama87I CYCGRG 2 cut(s) 432, 3404
Aor13HI TCCGGA 1 cut(s) 2038
AoxI GGCC 8 cut(s) 313, 896, 1657, 1883, 2332, 2558, 2658, 2695
ApaLI GTGCAC 1 cut(s) 1820
ApeKI GCWGC 9 cut(s) 284, 655, 1646, 2159, 2512, 2675, 2849, 3430, 3485
ApoI RAATTY 2 cut(s) 1003, 1375
ArsI GACNNNNNNTTYG 4 cut(s) 1360, 1392, 2591, 2623
Asp700I GAANNNNTTC 2 cut(s) 1173, 1934
AspS9I GGNCC 4 cut(s) 338, 1657, 2599, 3210
AsuC2I CCSGG 1 cut(s) 3087
AsuHPI GGTGA 5 cut(s) 607, 660, 2459, 2564, 3424
AsuII TTCGAA 1 cut(s) 1379
AsuNHI GCTAGC 1 cut(s) 1909
AvaI CYCGRG 2 cut(s) 432, 3404
AvaII GGWCC 3 cut(s) 338, 2599, 3210
AxyI CCTNAGG 1 cut(s) 3108
BaeGI GKGCMC 2 cut(s) 1824, 2765
BaeI ACNNNNGTAYC 4 cut(s) 366, 399, 2094, 2127
BalI TGGCCA 3 cut(s) 315, 1885, 2334
BamHI GGATCC 2 cut(s) 484, 750
BanII GRGCYC 1 cut(s) 1290
BarI GAAGNNNNNNTAC 2 cut(s) 2304, 2336
BauI CACGAG 1 cut(s) 1703
BbrPI CACGTG 2 cut(s) 501, 1586
BbsI GAAGAC 7 cut(s) 1243, 1451, 1578, 2214, 2456, 2949, 3146
Bbv12I GWGCWC 4 cut(s) 527, 813, 1290, 1824
BbvI GCAGC 9 cut(s) 271, 667, 1633, 2171, 2499, 2687, 2836, 3417, 3497
BccI CCATC 8 cut(s) 305, 1478, 1603, 1648, 1892, 1894, 3065, 3408
BceAI ACGGC 3 cut(s) 304, 2145, 2682
BcgI CGANNNNNNTGC 2 cut(s) 2714, 2748
BciT130I CCWGG 7 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714
BciVI GTATCC 2 cut(s) 917, 2313
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 1 cut(s) 3087
BcoDI GTCTC 6 cut(s) 1539, 1914, 2255, 2356, 2397, 2399
BcuI ACTAGT 1 cut(s) 1240
BfaI CTAG 8 cut(s) 998, 1241, 1520, 1650, 1910, 2030, 2306, 2846
BfmI CTRYAG 6 cut(s) 285, 1686, 1825, 2157, 2364, 2856
BfrI CTTAAG 1 cut(s) 1808
BfuAI ACCTGC 2 cut(s) 1847, 2168
BfuI GTATCC 2 cut(s) 917, 2313
BglII AGATCT 2 cut(s) 1962, 2416
BlpI GCTNAGC 1 cut(s) 3579
Bme1390I CCNGG 8 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714, 3087
Bme18I GGWCC 3 cut(s) 338, 2599, 3210
BmeT110I CYCGRG 2 cut(s) 432, 3404
BmgBI CACGTC 1 cut(s) 2782
BmgT120I GGNCC 4 cut(s) 338, 1657, 2599, 3210
BmiI GGNNCC 8 cut(s) 228, 339, 486, 752, 933, 1722, 2600, 2812
BmrFI CCNGG 8 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714, 3087
BmrI ACTGGG 1 cut(s) 195
BmtI GCTAGC 1 cut(s) 1913
BmuI ACTGGG 1 cut(s) 195
BpiI GAAGAC 7 cut(s) 1243, 1451, 1578, 2214, 2456, 2949, 3146
BplI GAGNNNNNCTC 4 cut(s) 883, 915, 1208, 1240
Bpu1102I GCTNAGC 1 cut(s) 3579
Bpu14I TTCGAA 1 cut(s) 1379
BpuEI CTTGAG 3 cut(s) 431, 756, 1088
BpuMI CCSGG 1 cut(s) 3087
Bsa29I ATCGAT 1 cut(s) 2724
BsaAI YACGTR 2 cut(s) 501, 1586
BsaBI GATNNNNATC 1 cut(s) 741
BsaHI GRCGYC 1 cut(s) 1086
BsaI GGTCTC 3 cut(s) 1539, 2255, 2397
BsaWI WCCGGW 2 cut(s) 2038, 3513
BsaXI ACNNNNNCTCC 2 cut(s) 923, 953
Bse1I ACTGG 5 cut(s) 201, 1033, 1130, 2841, 3450
Bse21I CCTNAGG 1 cut(s) 3108
Bse3DI GCAATG 3 cut(s) 299, 2445, 2779
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 2038
BseBI CCWGG 7 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714
BseCI ATCGAT 1 cut(s) 2724
BseJI GATNNNNATC 1 cut(s) 741
BseMI GCAATG 3 cut(s) 299, 2445, 2779
BseNI ACTGG 5 cut(s) 201, 1033, 1130, 2841, 3450
BseRI GAGGAG 3 cut(s) 141, 1660, 3356
BseSI GKGCMC 2 cut(s) 1824, 2765
BseXI GCAGC 9 cut(s) 271, 667, 1633, 2171, 2499, 2687, 2836, 3417, 3497
BseYI CCCAGC 1 cut(s) 2508
BsgI GTGCAG 2 cut(s) 2196, 3194
Bsh1236I CGCG 2 cut(s) 823, 2211
BshFI GGCC 8 cut(s) 315, 898, 1659, 1885, 2334, 2560, 2660, 2697
BshVI ATCGAT 1 cut(s) 2724
BsiHKAI GWGCWC 4 cut(s) 527, 813, 1290, 1824
BsiHKCI CYCGRG 2 cut(s) 432, 3404
BsiSI CCGG 4 cut(s) 2039, 2657, 3086, 3514
BslFI GGGAC 3 cut(s) 433, 701, 2612
BsmAI GTCTC 6 cut(s) 1539, 1914, 2255, 2356, 2397, 2399
BsmFI GGGAC 3 cut(s) 433, 701, 2612
BsmI GAATGC 3 cut(s) 957, 1523, 3265
BsnI GGCC 8 cut(s) 315, 898, 1659, 1885, 2334, 2560, 2660, 2697
Bso31I GGTCTC 3 cut(s) 1539, 2255, 2397
BsoBI CYCGRG 2 cut(s) 432, 3404
Bsp119I TTCGAA 1 cut(s) 1379
Bsp1286I GDGCHC 5 cut(s) 527, 813, 1290, 1824, 2765
Bsp13I TCCGGA 1 cut(s) 2038
Bsp1720I GCTNAGC 1 cut(s) 3579
Bsp19I CCATGG 2 cut(s) 276, 2814
BspANI GGCC 8 cut(s) 315, 898, 1659, 1885, 2334, 2560, 2660, 2697
BspDI ATCGAT 1 cut(s) 2724
BspEI TCCGGA 1 cut(s) 2038
BspFNI CGCG 2 cut(s) 823, 2211
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 8 cut(s) 228, 339, 486, 752, 933, 1722, 2600, 2812
BspMAI CTGCAG 3 cut(s) 289, 2161, 2860
BspMI ACCTGC 2 cut(s) 1847, 2168
BspOI GCTAGC 1 cut(s) 1913
BspPI GGATC 8 cut(s) 479, 492, 737, 745, 758, 2628, 3097, 3431
BspQI GCTCTTC 1 cut(s) 1463
BspT104I TTCGAA 1 cut(s) 1379
BspTI CTTAAG 1 cut(s) 1808
BspTNI GGTCTC 3 cut(s) 1539, 2255, 2397
BsrBI CCGCTC 1 cut(s) 1551
BsrDI GCAATG 3 cut(s) 299, 2445, 2779
BsrI ACTGG 5 cut(s) 201, 1033, 1130, 2841, 3450
BssNI GRCGYC 1 cut(s) 1086
BssSI CACGAG 1 cut(s) 1703
BssT1I CCWWGG 4 cut(s) 276, 746, 1080, 2814
Bst2BI CACGAG 1 cut(s) 1703
Bst2UI CCWGG 7 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714
Bst4CI ACNGT 7 cut(s) 382, 613, 715, 1514, 1687, 1925, 2361
Bst6I CTCTTC 4 cut(s) 1463, 2676, 2681, 3180
BstACI GRCGYC 1 cut(s) 1086
BstAFI CTTAAG 1 cut(s) 1808
BstAPI GCANNNNNTGC 3 cut(s) 1643, 2138, 2769
BstBAI YACGTR 2 cut(s) 501, 1586
BstBI TTCGAA 1 cut(s) 1379
BstC8I GCNNGC 4 cut(s) 317, 558, 660, 1911
BstDSI CCRYGG 3 cut(s) 276, 821, 2814
BstENI CCTNNNNNAGG 3 cut(s) 1975, 2547, 3284
BstFNI CGCG 2 cut(s) 823, 2211
BstMAI GTCTC 6 cut(s) 1539, 1914, 2255, 2356, 2397, 2399
BstNI CCWGG 7 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714
BstNSI RCATGY 2 cut(s) 560, 2524
BstSCI CCNGG 8 cut(s) 154, 477, 718, 1970, 1987, 2470, 2712, 3085
BstSFI CTRYAG 6 cut(s) 285, 1686, 1825, 2157, 2364, 2856
BstSLI GKGCMC 2 cut(s) 1824, 2765
BstUI CGCG 2 cut(s) 823, 2211
BstV1I GCAGC 9 cut(s) 271, 667, 1633, 2171, 2499, 2687, 2836, 3417, 3497
BstV2I GAAGAC 7 cut(s) 1243, 1451, 1578, 2214, 2456, 2949, 3146
BstX2I RGATCY 5 cut(s) 484, 750, 1962, 2416, 3436
BstYI RGATCY 5 cut(s) 484, 750, 1962, 2416, 3436
Bsu15I ATCGAT 1 cut(s) 2724
Bsu36I CCTNAGG 1 cut(s) 3108
BsuI GTATCC 2 cut(s) 917, 2313
BsuRI GGCC 8 cut(s) 315, 898, 1659, 1885, 2334, 2560, 2660, 2697
BsuTUI ATCGAT 1 cut(s) 2724
BtgI CCRYGG 3 cut(s) 276, 821, 2814
BtgZI GCGATG 1 cut(s) 3476
BtrI CACGTC 1 cut(s) 2782
BtsI GCAGTG 5 cut(s) 525, 555, 2154, 2590, 2853
BtsIMutI CAGTG 8 cut(s) 525, 555, 759, 1683, 1713, 2154, 2590, 2853
BveI ACCTGC 2 cut(s) 1847, 2168
Cac8I GCNNGC 4 cut(s) 317, 558, 660, 1911
CciI TCATGA 1 cut(s) 1204
Cfr13I GGNCC 4 cut(s) 338, 1657, 2599, 3210
Cfr42I CCGCGG 1 cut(s) 824
ClaI ATCGAT 1 cut(s) 2724
CseI GACGC 2 cut(s) 3077, 3572
CspCI CAANNNNNGTGG 2 cut(s) 2842, 2877
DraI TTTAAA 2 cut(s) 1459, 1492
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 4 cut(s) 313, 1883, 2332, 2658
Eam1104I CTCTTC 4 cut(s) 1463, 2676, 2681, 3180
EarI CTCTTC 4 cut(s) 1463, 2676, 2681, 3180
Ecl136II GAGCTC 1 cut(s) 1288
Eco130I CCWWGG 4 cut(s) 276, 746, 1080, 2814
Eco24I GRGCYC 1 cut(s) 1290
Eco31I GGTCTC 3 cut(s) 1539, 2255, 2397
Eco32I GATATC 1 cut(s) 1222
Eco47I GGWCC 3 cut(s) 338, 2599, 3210
Eco53kI GAGCTC 1 cut(s) 1288
Eco57I CTGAAG 7 cut(s) 483, 1590, 2208, 2265, 2700, 3281, 3360
Eco72I CACGTG 2 cut(s) 501, 1586
Eco81I CCTNAGG 1 cut(s) 3108
Eco88I CYCGRG 2 cut(s) 432, 3404
EcoICRI GAGCTC 1 cut(s) 1288
EcoNI CCTNNNNNAGG 3 cut(s) 1975, 2547, 3284
EcoO109I RGGNCCY 2 cut(s) 338, 2599
EcoRII CCWGG 7 cut(s) 154, 477, 718, 1970, 1987, 2470, 2712
EcoRV GATATC 1 cut(s) 1222
EcoT14I CCWWGG 4 cut(s) 276, 746, 1080, 2814
EcoT22I ATGCAT 2 cut(s) 558, 2667
EcoT38I GRGCYC 1 cut(s) 1290
ErhI CCWWGG 4 cut(s) 276, 746, 1080, 2814
FalI AAGNNNNNCTT 2 cut(s) 1922, 1954
FaqI GGGAC 3 cut(s) 433, 701, 2612
FauI CCCGC 2 cut(s) 1332, 2204
FauNDI CATATG 1 cut(s) 1678
FbaI TGATCA 1 cut(s) 564
FriOI GRGCYC 1 cut(s) 1290
FspBI CTAG 8 cut(s) 998, 1241, 1520, 1650, 1910, 2030, 2306, 2846
GsaI CCCAGC 1 cut(s) 2512
HaeIII GGCC 8 cut(s) 315, 898, 1659, 1885, 2334, 2560, 2660, 2697
HapII CCGG 4 cut(s) 2039, 2657, 3086, 3514
HgaI GACGC 2 cut(s) 3077, 3572
Hin1I GRCGYC 1 cut(s) 1086
HincII GTYRAC 3 cut(s) 1044, 1711, 2707
HindII GTYRAC 3 cut(s) 1044, 1711, 2707
HindIII AAGCTT 4 cut(s) 1157, 1190, 2574, 3024
HpaII CCGG 4 cut(s) 2039, 2657, 3086, 3514
HphI GGTGA 5 cut(s) 607, 660, 2459, 2564, 3424
Hpy166II GTNNAC 5 cut(s) 147, 1044, 1711, 1822, 2707
Hpy8I GTNNAC 5 cut(s) 147, 1044, 1711, 1822, 2707
Hpy99I CGWCG 2 cut(s) 2786, 3140
HpyAV CCTTC 9 cut(s) 458, 541, 965, 1408, 1925, 2027, 2280, 2908, 3364
HpyCH4III ACNGT 7 cut(s) 382, 613, 715, 1514, 1687, 1925, 2361
HpyCH4IV ACGT 7 cut(s) 500, 886, 1086, 1452, 1585, 1849, 2781
HpySE526I ACGT 7 cut(s) 500, 886, 1086, 1452, 1585, 1849, 2781
Hsp92I GRCGYC 1 cut(s) 1086
Kpn2I TCCGGA 1 cut(s) 2038
Ksp22I TGATCA 1 cut(s) 564
KspI CCGCGG 1 cut(s) 824
LguI GCTCTTC 1 cut(s) 1463
LmnI GCTCC 4 cut(s) 226, 931, 1408, 2810
Lsp1109I GCAGC 9 cut(s) 271, 667, 1633, 2171, 2499, 2687, 2836, 3417, 3497
MaeI CTAG 8 cut(s) 998, 1241, 1520, 1650, 1910, 2030, 2306, 2846
MaeII ACGT 7 cut(s) 500, 886, 1086, 1452, 1585, 1849, 2781
MbiI CCGCTC 1 cut(s) 1551
MfeI CAATTG 2 cut(s) 568, 783
MflI RGATCY 5 cut(s) 484, 750, 1962, 2416, 3436
MhlI GDGCHC 5 cut(s) 527, 813, 1290, 1824, 2765
MlsI TGGCCA 3 cut(s) 315, 1885, 2334
MluNI TGGCCA 3 cut(s) 315, 1885, 2334
MlyI GAGTC 4 cut(s) 142, 1210, 3455, 3533
MmeI TCCRAC 3 cut(s) 564, 1317, 1794
Mox20I TGGCCA 3 cut(s) 315, 1885, 2334
Mph1103I ATGCAT 2 cut(s) 558, 2667
MroI TCCGGA 1 cut(s) 2038
MroXI GAANNNNTTC 2 cut(s) 1173, 1934
MscI TGGCCA 3 cut(s) 315, 1885, 2334
MslI CAYNNNNRTG 3 cut(s) 1020, 2523, 2670
Msp20I TGGCCA 3 cut(s) 315, 1885, 2334
MspA1I CMGCKG 6 cut(s) 266, 658, 823, 2512, 2678, 3488
MspCI CTTAAG 1 cut(s) 1808
MspI CCGG 4 cut(s) 2039, 2657, 3086, 3514
MspR9I CCNGG 8 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714, 3087
MssI GTTTAAAC 1 cut(s) 1492
MunI CAATTG 2 cut(s) 568, 783
Mva1269I GAATGC 3 cut(s) 957, 1523, 3265
MvaI CCWGG 7 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714
MvnI CGCG 2 cut(s) 823, 2211
NciI CCSGG 1 cut(s) 3087
NcoI CCATGG 2 cut(s) 276, 2814
NdeI CATATG 1 cut(s) 1678
NheI GCTAGC 1 cut(s) 1909
NlaIV GGNNCC 8 cut(s) 228, 339, 486, 752, 933, 1722, 2600, 2812
NmuCI GTSAC 6 cut(s) 34, 2585, 3119, 3193, 3200, 3516
NsiI ATGCAT 2 cut(s) 558, 2667
NspI RCATGY 2 cut(s) 560, 2524
NspV TTCGAA 1 cut(s) 1379
OliI CACNNNNGTG 1 cut(s) 1020
PaeI GCATGC 1 cut(s) 560
PagI TCATGA 1 cut(s) 1204
PasI CCCWGGG 1 cut(s) 2471
PciI ACATGT 1 cut(s) 2520
PciSI GCTCTTC 1 cut(s) 1463
PctI GAATGC 3 cut(s) 957, 1523, 3265
PdmI GAANNNNTTC 2 cut(s) 1173, 1934
PfoI TCCNGGA 1 cut(s) 1970
PleI GAGTC 4 cut(s) 142, 1209, 3454, 3533
PmaCI CACGTG 2 cut(s) 501, 1586
PmeI GTTTAAAC 1 cut(s) 1492
PmlI CACGTG 2 cut(s) 501, 1586
PpsI GAGTC 4 cut(s) 142, 1209, 3454, 3533
Ppu21I YACGTR 2 cut(s) 501, 1586
PpuMI RGGWCCY 2 cut(s) 338, 2599
PscI ACATGT 1 cut(s) 2520
PsiI TTATAA 3 cut(s) 390, 579, 3273
Psp124BI GAGCTC 1 cut(s) 1290
Psp5II RGGWCCY 2 cut(s) 338, 2599
Psp6I CCWGG 7 cut(s) 154, 477, 718, 1970, 1987, 2470, 2712
PspCI CACGTG 2 cut(s) 501, 1586
PspFI CCCAGC 1 cut(s) 2508
PspGI CCWGG 7 cut(s) 154, 477, 718, 1970, 1987, 2470, 2712
PspN4I GGNNCC 8 cut(s) 228, 339, 486, 752, 933, 1722, 2600, 2812
PspPI GGNCC 4 cut(s) 338, 1657, 2599, 3210
PspPPI RGGWCCY 2 cut(s) 338, 2599
PsrI GAACNNNNNNTAC 2 cut(s) 2019, 2051
PstI CTGCAG 3 cut(s) 289, 2161, 2860
PsuI RGATCY 5 cut(s) 484, 750, 1962, 2416, 3436
PvuII CAGCTG 5 cut(s) 266, 658, 2512, 2678, 3488
RseI CAYNNNNRTG 3 cut(s) 1020, 2523, 2670
SacI GAGCTC 1 cut(s) 1290
SacII CCGCGG 1 cut(s) 824
SapI GCTCTTC 1 cut(s) 1463
Sau96I GGNCC 4 cut(s) 338, 1657, 2599, 3210
SchI GAGTC 4 cut(s) 142, 1210, 3455, 3533
ScrFI CCNGG 8 cut(s) 156, 479, 720, 1972, 1989, 2472, 2714, 3087
SduI GDGCHC 5 cut(s) 527, 813, 1290, 1824, 2765
SfcI CTRYAG 6 cut(s) 285, 1686, 1825, 2157, 2364, 2856
Sfr303I CCGCGG 1 cut(s) 824
SfuI TTCGAA 1 cut(s) 1379
SgrBI CCGCGG 1 cut(s) 824
SinI GGWCC 3 cut(s) 338, 2599, 3210
SmiMI CAYNNNNRTG 3 cut(s) 1020, 2523, 2670
SmlI CTYRAG 4 cut(s) 410, 771, 1103, 1808
SmoI CTYRAG 4 cut(s) 410, 771, 1103, 1808
SpeI ACTAGT 1 cut(s) 1240
SphI GCATGC 1 cut(s) 560
SspI AATATT 1 cut(s) 2049
SspMI CTAG 8 cut(s) 998, 1241, 1520, 1650, 1910, 2030, 2306, 2846
SstI GAGCTC 1 cut(s) 1290
StyD4I CCNGG 8 cut(s) 154, 477, 718, 1970, 1987, 2470, 2712, 3085
StyI CCWWGG 4 cut(s) 276, 746, 1080, 2814
TaaI ACNGT 7 cut(s) 382, 613, 715, 1514, 1687, 1925, 2361
TaiI ACGT 7 cut(s) 503, 889, 1089, 1455, 1588, 1852, 2784
TaqI TCGA 7 cut(s) 790, 805, 1379, 1918, 2724, 2963, 3537
TatI WGTACW 1 cut(s) 2604
TauI GCSGC 3 cut(s) 2203, 2563, 3461
TscAI CASTG 8 cut(s) 532, 555, 766, 1690, 1720, 2161, 2590, 2860
TseFI GTSAC 6 cut(s) 34, 2585, 3119, 3193, 3200, 3516
TseI GCWGC 9 cut(s) 284, 655, 1646, 2159, 2512, 2675, 2849, 3430, 3485
Tsp45I GTSAC 6 cut(s) 34, 2585, 3119, 3193, 3200, 3516
TspGWI ACGGA 4 cut(s) 743, 899, 1773, 2014
TspRI CASTG 8 cut(s) 532, 555, 766, 1690, 1720, 2161, 2590, 2860
Vha464I CTTAAG 1 cut(s) 1808
VneI GTGCAC 1 cut(s) 1820
VpaK11BI GGWCC 3 cut(s) 338, 2599, 3210
XagI CCTNNNNNAGG 3 cut(s) 1975, 2547, 3284
XapI RAATTY 2 cut(s) 1003, 1375
XceI RCATGY 2 cut(s) 560, 2524
XmnI GAANNNNTTC 2 cut(s) 1173, 1934
XspI CTAG 8 cut(s) 998, 1241, 1520, 1650, 1910, 2030, 2306, 2846
ZraI GACGTC 1 cut(s) 1087
Zsp2I ATGCAT 2 cut(s) 558, 2667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.