MD15G1124800.v1.1
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008702
Physical Location & Seq
Forward (+)
9057097 .. 9062894
5798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1124800.v1.1.491

Sequence Viewer

Length: 3594 bp
ATGGAATCTGAAGCAAGTGAAACTAGCACAGTGGTAACTCAACTTAGTTTTGGTGGATTTGGTAGACATGTGAAGGCAAGCGATCTTGTAACGTACTTGGAAGATGAAATTGGGCTTGTATATAGGTGCAGGTTGAAAACATCTTGGACCCCTCAAGAGTCCTTTCCAAATTTTGAGCTCATTGATCCTTCAAATATTGCAAGAACAAATGATTATATCATGGTGGAGCCTCATGCATTTGTGCATTTTGCCATGCCTGAGTCAGTAACTTTTGCTATGGATGCAGCAGGGCGTTCTCAACTCTTTTACAACAATGAACCATTGAAAGTAAGTTTGGGTCCTGAGAATCCATATTTCTTGAATAGGAGGAGGAGGGATAAAACTCCATTCAAGTTATCTGATGTATGTGTCGACATTGGAAGTTTAGTTCGTCAAGATGAGTTTTTTGTCGCTTGGAGAGGACCCACTTATGGTGTTGACTTTATTGTGGATCCTTTTGATGGAACATGCAAATTTTGTTTTACCAGAGACACTGCATTTTCATTTAAGGGGATAAAGAAGCATGCAGTCATTAAATGCGATTTTATGGTAGAGTTCTTGGTAAGAGACGTTTCTGAAATCAAGCAATACACAGATACTGCATATCATGTAATTCTGTTGCGGCTGGCTTCATCACCCTGGGTTTCGTATAGAACTGCTGATGATGACATTGACCAATCAGTCCCATTTAATCTGTTGGATGATGATGATCCTTGGATTAGAACCACGGATTTTACTCCTAGTGGGGCTATTGGGCGTTGTAATTGCTACAGAGTTTCAATACCCCCCCGACATGGTGCAAAGTTGAAGAGGACCATGGATTATCTTCGAGAACACAGGGTGCGGGAGGTTTGCCTCAAACGGCCTCCTAAGGTTCAAAACGAACCTGATTTTGGAATGCCTGCTTCGGATCCTTTCTTCTGTATTCATTATAAGGATGATATTTCGTTTGAGATAATGTTCTTGGTTAATGCAGTAATGCACAAGGGCATTTTTAATCAGCACCAGTTGTCGGACAGTTTCTTTGGTTTACTGAGGAGCCAACCAAAGGAGGTCAATGTGGCTGCATTGAAGCACTTGTGCACTTACAAGCACCCAGAATTTGATGCCTGTAAGAGACTGAAAGTTGTTCAAGATTGGTTGCTGAAGAACCCCAAGCTTTTCAAGGATCCTAAGCGATTGGATGACATTTCTGAAGTTAGGAGGTTGATTATCACCCCAACTAAAGCCTACTGTCTTCCGCCAGAGGTTGAACTTTCCAATAGGGTTCTTAGAAAGTATAAGGAAGTTGCTGATCGGTTCTTAAGGGTTACCTTTATGGATGAAGGTATGCAGACAATCAACTCTCATGTTCTGAACTCTTATGTTGCTCCTATTGTGAAGGAAATTATGACGAACGCCTCTCCTCAGAAAACAAATGTATTCAAAAGGGTGAAGACAATTTTGACTGACGGCTTTTACTTGTGTAGTCGTAAGTACTCTTTCTTGGCCTTCTCATCCAATCAATTGAAGGATCGTTCTGCCTGGTTTTTTGCTGAAGACATGAACATTGCTATCCTTGACATCAAAAAATGGATGGGAAAGTTCGCCAACAAAAATATTGCTAAATGTGCTGCTAGGATGGGCCTATGCTTTTCTTCTACATATGCAACGGTAGACGTTCCATTAAGTGAAGTTAATTTTGACCTTCCAGATATCAAGAGGAATGAGTATGATTTTTCTGATGGGATTGGTAAGATTACTCCTGACCTCGCAATGAAAGTCGCAGAGAAGTTGAAACTCGACAGAGATCCACCTTGTGCATATCAAATAAGATATGCTGGCTGTAAAGGGGTTGTGGCTTGTTGGCCATCCAATGGTGATGGGTACCGGCTTTCTTTAAGGCCTAGTATGAACAAGTTTAAGTCATGCCATACTATACTGGAAATTTGTTCTTGGACTAGGTTGCAGCCGGGTTTTCTAAACAGGCAGATCATCACATTACTTTCTGCACTAGATGTTCCAGATGAGATATTCTGGAGAAAGCAGGAGAGAATGGTTTTAAAGTTAAACCGGATGCTTGTGGACACAGATGTGGCATTTGATGTTCTCACTGGCTCTTGCGCTGAGCAAGGAAATGCAGCAGCTATAATGCTGAGTGCTGGTTTCAAGCCCCAGACAGAACCTCATCTACGGGGCATGTTAACCTGTATACGAGCCGCTCAGCTTTGGGGCCTTAGGGAAAAGGCTAGGATTTTTGTTCCTTCTGGAAGATGGCTTATGGGATGTCTAGATGAGCTAGGGGTACTTGAGCAAGGCCAGTGCTTTGTCCAAGTGTCTACCCCGTCACTACAGAATTGTTTTGCCAATCATGGCTCTAGGTTTGATAAGATTGAGAAGAATCTTCAAGTGATTAAGGGGCTTGTTGTGATAGCAAAGAATCCTTGCCTTCATCCTGGAGATATAAGGATTCTTGAAGCAGTTGATGCCCCTGAGTTACGCCACTTGCATGATTGCATTCTTTTCCCTCAGAAGGGTGATAGGCCCCACACAAATGAAGCTTCTGGAAGTGACCTCGATGGGGACCTTTACTTTGTTACTTGGGATGAAGATCTTATTCCCCCAAGTAAGAAGAGCTGGACTCCTATGATGTATGATCCTCAAGAAGCCAAACCTCATGGGCGTCCTATAACTCAGATGGATATTATAAATTTTTTTGCGAAAAACATGGTGAATGAGAACTTAGGGGCTATATGCAATGCACATGTGGTTCATGCTGACCGTAGTGATTATGGGGCTATGGATGAGAACTGCCTTCTATTAGCTGAGTATGCAGCATTAGCAGTTGATTTTCCGAAAACTGGAAAGACTGTTGCCTTGCCAGCACATCTAAAGCCGAAGATGTACCCTGATTTCATGGGGAAGGAGGAGCACCAGACGTACAAGTCAACTAAAATTTTGGGGCGACTATATCGCCACATTAGAGATGCTTATGACGAAGAAATGGCAACCTCTTTGGAGCTGAATTATACTCCTGGTGACATCCCATATGATATGGATCTGGAGATTCCTGGAGCCACTGATTTTATAGCTGACGCGTGGGAGAAAAAGTGCTCTTATGATGGCCAGGTGAAGGGTCTAATGGGGCAATACAAAGTTAAGAGAGAAGAAGAGATTGTTACTGGGCATGTTTGGTCCATCCCCAAATCCAACAGCAAGAAGCAAGGGGAGCTGAAGGAGCGGTTGAAGCAATCCTACGGTGCTCTTAAAAAAGAATTCAGGCTAATTTTTGAGAACAGGGATCCAAATATTGAATCTCTTACTGATGATGAGAAGAGTATACTGTATGAACAGAAGGCATCGGCATGGTACCAGGTGACTTACCACCCGAAATGGGTGAAAAGATCAATCCATTTGCAAGAGCCGGATGTTGCTGGAAATGTGGCGATGTTGAGCTTTGCCTGGATTACAGCGGACTACCTTGCACGAATCAAGATCAAGCGCCGCGGAGTGGAACATATTGATTCTCCGAAGCCTATCAACTCTTTGTCGAAGTACCTAGCTGATCGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1198

Amino Acids

136.86

Weight (kDa)

7.81

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_RDR6 PF24572 7 - 111 5.9e-30 RNA-dependent RNA polymerase 6, RNA-binding domain
RDR6_2nd PF24577 131 - 294 2e-86 RNA-dependent RNA polymerase 6 second domain
PH_RDR2 PF24823 195 - 286 2.2e-06 RDR2 PH-like domain
RdRP_helical PF26252 318 - 400 3.2e-23 RNA dependent RNA polymerase, helical domain
RdRP PF05183 418 - 1000 1e-187 RNA dependent RNA polymerase
RdRP_head PF26253 1022 - 1180 4.1e-43 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 972, 2726
AasI GACNNNNNNGTC 2 cut(s) 719, 2962
Acc36I ACCTGC 1 cut(s) 120
Acc65I GGTACC 2 cut(s) 1905, 3387
AccB1I GGYRCC 2 cut(s) 1905, 3387
AccB7I CCANNNNNTGG 1 cut(s) 1895
AccBSI CCGCTC 2 cut(s) 2240, 3259
AccI GTMKAC 6 cut(s) 64, 411, 1695, 2230, 2357, 3358
AccII CGCG 2 cut(s) 3116, 3525
AciI CCGC 8 cut(s) 661, 883, 1280, 2238, 3259, 3491, 3523, 3525
AcoI YGGCCR 2 cut(s) 1886, 3142
AcsI RAATTY 7 cut(s) 169, 512, 1139, 1965, 2728, 2973, 3293
AcuI CTGAAG 5 cut(s) 30, 1205, 1254, 1596, 3272
AcyI GRCGYC 1 cut(s) 2701
AdeI CACNNNGTG 2 cut(s) 880, 1838
AfaI GTAC 8 cut(s) 95, 1517, 1907, 2325, 2924, 2960, 3389, 3575
AflII CTTAAG 1 cut(s) 1342
AflIII ACRYGT 3 cut(s) 67, 2782, 3114
AjnI CCWGG 8 cut(s) 677, 1562, 2473, 3052, 3088, 3144, 3390, 3479
AjuI GAANNNNNNNTTGG 6 cut(s) 93, 125, 317, 349, 915, 947
AleI CACNNNNGTG 1 cut(s) 2111
AloI GAACNNNNNNTCC 2 cut(s) 411, 443
Alw21I GWGCWC 5 cut(s) 180, 1124, 2952, 3134, 3283
Alw26I GTCTC 3 cut(s) 522, 600, 1150
Alw44I GTGCAC 1 cut(s) 1120
AlwNI CAGNNNCTG 1 cut(s) 638
AoxI GGCC 9 cut(s) 902, 1527, 1663, 1886, 1922, 2251, 2335, 2561, 3142
ApaLI GTGCAC 1 cut(s) 1120
ApeKI GCWGC 7 cut(s) 284, 1103, 1652, 1987, 2159, 2162, 2852
ApoI RAATTY 7 cut(s) 169, 512, 1139, 1965, 2728, 2973, 3293
ArsI GACNNNNNNTTYG 4 cut(s) 1046, 1078, 2594, 2626
Asp718I GGTACC 2 cut(s) 1905, 3387
AspLEI GCGC 2 cut(s) 2144, 3522
AspS9I GGNCC 9 cut(s) 147, 338, 461, 852, 1663, 2251, 2562, 2602, 3213
AsuC2I CCSGG 1 cut(s) 1992
AvaII GGWCC 6 cut(s) 147, 338, 461, 852, 2602, 3213
AxyI CCTNAGG 2 cut(s) 909, 2255
BaeGI GKGCMC 1 cut(s) 1124
BalI TGGCCA 2 cut(s) 1888, 3144
BamHI GGATCC 4 cut(s) 490, 949, 1207, 3319
BanI GGYRCC 2 cut(s) 1905, 3387
BanII GRGCYC 1 cut(s) 180
BarI GAAGNNNNNNTAC 2 cut(s) 3257, 3289
BbsI GAAGAC 3 cut(s) 1268, 1481, 1584
Bbv12I GWGCWC 5 cut(s) 180, 1124, 2952, 3134, 3283
BbvI GCAGC 7 cut(s) 296, 1090, 1639, 1999, 2171, 2174, 2864
BceAI ACGGC 2 cut(s) 917, 1507
BciT130I CCWGG 8 cut(s) 679, 1564, 2475, 3054, 3090, 3146, 3392, 3481
BcnI CCSGG 1 cut(s) 1992
BcoDI GTCTC 3 cut(s) 522, 600, 1150
BfmI CTRYAG 2 cut(s) 808, 2369
BfoI RGCGCY 1 cut(s) 3523
BfrI CTTAAG 1 cut(s) 1342
BfuAI ACCTGC 1 cut(s) 120
BglII AGATCT 1 cut(s) 2629
BlpI GCTNAGC 2 cut(s) 2145, 2241
BmcAI AGTACT 1 cut(s) 1517
Bme1390I CCNGG 9 cut(s) 679, 1564, 1992, 2475, 3054, 3090, 3146, 3392, 3481
Bme18I GGWCC 6 cut(s) 147, 338, 461, 852, 2602, 3213
BmgT120I GGNCC 9 cut(s) 147, 338, 461, 852, 1663, 2251, 2562, 2602, 3213
BmrFI CCNGG 9 cut(s) 679, 1564, 1992, 2475, 3054, 3090, 3146, 3392, 3481
BmrI ACTGGG 1 cut(s) 3210
BmsI GCATC 6 cut(s) 271, 1135, 2085, 2494, 2995, 3386
BmuI ACTGGG 1 cut(s) 3210
BpiI GAAGAC 3 cut(s) 1268, 1481, 1584
BplI GAGNNNNNCTC 2 cut(s) 2644, 2676
BpmI CTGGAG 4 cut(s) 2077, 2496, 3101, 3111
Bpu10I CCTNAGC 1 cut(s) 1212
Bpu1102I GCTNAGC 2 cut(s) 2145, 2241
BpuEI CTTGAG 3 cut(s) 138, 2348, 2664
BpuMI CCSGG 1 cut(s) 1992
BsaBI GATNNNNATC 3 cut(s) 747, 2628, 3075
BsaHI GRCGYC 1 cut(s) 2701
BsaJI CCNNGG 6 cut(s) 677, 678, 752, 765, 855, 3523
BsaWI WCCGGW 1 cut(s) 2091
BsaXI ACNNNNNCTCC 2 cut(s) 3113, 3143
Bse118I RCCGGY 1 cut(s) 1908
Bse1I ACTGG 6 cut(s) 1045, 1965, 2137, 2338, 2884, 3205
Bse21I CCTNAGG 2 cut(s) 909, 2255
Bse3DI GCAATG 3 cut(s) 1587, 1800, 2782
Bse8I GATNNNNATC 3 cut(s) 747, 2628, 3075
BseBI CCWGG 8 cut(s) 679, 1564, 2475, 3054, 3090, 3146, 3392, 3481
BseDI CCNNGG 6 cut(s) 677, 678, 752, 765, 855, 3523
BseJI GATNNNNATC 3 cut(s) 747, 2628, 3075
BseMI GCAATG 3 cut(s) 1587, 1800, 2782
BseNI ACTGG 6 cut(s) 1045, 1965, 2137, 2338, 2884, 3205
BseRI GAGGAG 5 cut(s) 382, 385, 1090, 1434, 2960
BseSI GKGCMC 1 cut(s) 1124
BseXI GCAGC 7 cut(s) 296, 1090, 1639, 1999, 2171, 2174, 2864
BsgI GTGCAG 2 cut(s) 148, 2013
Bsh1236I CGCG 2 cut(s) 3116, 3525
BshFI GGCC 9 cut(s) 904, 1529, 1665, 1888, 1924, 2253, 2337, 2563, 3144
BshNI GGYRCC 2 cut(s) 1905, 3387
BsiHKAI GWGCWC 5 cut(s) 180, 1124, 2952, 3134, 3283
BsiSI CCGG 4 cut(s) 1909, 1991, 2092, 3443
BslFI GGGAC 2 cut(s) 707, 2615
BsmAI GTCTC 3 cut(s) 522, 600, 1150
BsmBI CGTCTC 1 cut(s) 600
BsmFI GGGAC 2 cut(s) 707, 2615
BsmI GAATGC 2 cut(s) 942, 2535
BsnI GGCC 9 cut(s) 904, 1529, 1665, 1888, 1924, 2253, 2337, 2563, 3144
Bsp1286I GDGCHC 5 cut(s) 180, 1124, 2952, 3134, 3283
Bsp1720I GCTNAGC 2 cut(s) 2145, 2241
Bsp19I CCATGG 1 cut(s) 855
BspACI CCGC 8 cut(s) 661, 883, 1280, 2238, 3259, 3491, 3523, 3525
BspANI GGCC 9 cut(s) 904, 1529, 1665, 1888, 1924, 2253, 2337, 2563, 3144
BspFNI CGCG 2 cut(s) 3116, 3525
BspMI ACCTGC 1 cut(s) 120
BspQI GCTCTTC 1 cut(s) 2645
BspT107I GGYRCC 2 cut(s) 1905, 3387
BspTI CTTAAG 1 cut(s) 1342
BsrBI CCGCTC 2 cut(s) 2240, 3259
BsrDI GCAATG 3 cut(s) 1587, 1800, 2782
BsrFI RCCGGY 1 cut(s) 1908
BsrI ACTGG 6 cut(s) 1045, 1965, 2137, 2338, 2884, 3205
BssAI RCCGGY 1 cut(s) 1908
BssECI CCNNGG 6 cut(s) 677, 678, 752, 765, 855, 3523
BssNAI GTATAC 2 cut(s) 2231, 3359
BssNI GRCGYC 1 cut(s) 2701
BssT1I CCWWGG 2 cut(s) 752, 855
Bst1107I GTATAC 2 cut(s) 2231, 3359
Bst2UI CCWGG 8 cut(s) 679, 1564, 2475, 3054, 3090, 3146, 3392, 3481
Bst4CI ACNGT 8 cut(s) 31, 1058, 1274, 1693, 2801, 2890, 3278, 3363
Bst6I CTCTTC 4 cut(s) 842, 2645, 3183, 3347
BstACI GRCGYC 1 cut(s) 2701
BstAFI CTTAAG 1 cut(s) 1342
BstAPI GCANNNNNTGC 1 cut(s) 2504
BstC8I GCNNGC 6 cut(s) 79, 564, 666, 942, 1861, 2901
BstDSI CCRYGG 3 cut(s) 765, 855, 3523
BstEII GGTNACC 1 cut(s) 1348
BstFNI CGCG 2 cut(s) 3116, 3525
BstH2I RGCGCY 1 cut(s) 3523
BstHHI GCGC 2 cut(s) 2144, 3522
BstMAI GTCTC 3 cut(s) 522, 600, 1150
BstMWI GCNNNNNNNGC 9 cut(s) 281, 1649, 2504, 2849, 2858, 2900, 3247, 3256, 3265
BstNI CCWGG 8 cut(s) 679, 1564, 2475, 3054, 3090, 3146, 3392, 3481
BstNSI RCATGY 6 cut(s) 71, 510, 566, 2221, 2786, 3209
BstPI GGTNACC 1 cut(s) 1348
BstSCI CCNGG 9 cut(s) 677, 1562, 1990, 2473, 3052, 3088, 3144, 3390, 3479
BstSFI CTRYAG 2 cut(s) 808, 2369
BstSLI GKGCMC 1 cut(s) 1124
BstUI CGCG 2 cut(s) 3116, 3525
BstV1I GCAGC 7 cut(s) 296, 1090, 1639, 1999, 2171, 2174, 2864
BstV2I GAAGAC 3 cut(s) 1268, 1481, 1584
BstX2I RGATCY 7 cut(s) 490, 949, 1207, 1828, 2629, 3076, 3319
BstYI RGATCY 7 cut(s) 490, 949, 1207, 1828, 2629, 3076, 3319
BstZ17I GTATAC 2 cut(s) 2231, 3359
Bsu36I CCTNAGG 2 cut(s) 909, 2255
BsuRI GGCC 9 cut(s) 904, 1529, 1665, 1888, 1924, 2253, 2337, 2563, 3144
BtgI CCRYGG 3 cut(s) 765, 855, 3523
BtgZI GCGATG 1 cut(s) 3479
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 5 cut(s) 36, 531, 2130, 2345, 3096
BveI ACCTGC 1 cut(s) 120
Cac8I GCNNGC 6 cut(s) 79, 564, 666, 942, 1861, 2901
CaiI CAGNNNCTG 1 cut(s) 638
CfoI GCGC 2 cut(s) 2144, 3522
Cfr10I RCCGGY 1 cut(s) 1908
Cfr13I GGNCC 9 cut(s) 147, 338, 461, 852, 1663, 2251, 2562, 2602, 3213
Cfr42I CCGCGG 1 cut(s) 3526
CseI GACGC 2 cut(s) 2690, 3122
CsiI ACCWGGT 1 cut(s) 3390
Csp6I GTAC 8 cut(s) 94, 1516, 1906, 2324, 2923, 2959, 3388, 3574
CviQI GTAC 8 cut(s) 94, 1516, 1906, 2324, 2923, 2959, 3388, 3574
DraI TTTAAA 1 cut(s) 2082
DraIII CACNNNGTG 2 cut(s) 880, 1838
DrdI GACNNNNNNGTC 2 cut(s) 719, 2962
DseDI GACNNNNNNGTC 2 cut(s) 719, 2962
EaeI YGGCCR 2 cut(s) 1886, 3142
Eam1104I CTCTTC 4 cut(s) 842, 2645, 3183, 3347
EarI CTCTTC 4 cut(s) 842, 2645, 3183, 3347
EciI GGCGGA 1 cut(s) 1269
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 752, 855
Eco147I AGGCCT 1 cut(s) 1924
Eco24I GRGCYC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 1735
Eco47I GGWCC 6 cut(s) 147, 338, 461, 852, 2602, 3213
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 5 cut(s) 30, 1205, 1254, 1596, 3272
Eco81I CCTNAGG 2 cut(s) 909, 2255
Eco91I GGTNACC 1 cut(s) 1348
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 5 cut(s) 338, 461, 2251, 2562, 2602
EcoO65I GGTNACC 1 cut(s) 1348
EcoRI GAATTC 1 cut(s) 3293
EcoRII CCWGG 8 cut(s) 677, 1562, 2473, 3052, 3088, 3144, 3390, 3479
EcoRV GATATC 1 cut(s) 1735
EcoT14I CCWWGG 2 cut(s) 752, 855
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 752, 855
Esp3I CGTCTC 1 cut(s) 600
FaqI GGGAC 2 cut(s) 707, 2615
FauI CCCGC 1 cut(s) 876
FauNDI CATATG 2 cut(s) 1684, 3067
FblI GTMKAC 6 cut(s) 64, 411, 1695, 2230, 2357, 3358
FriOI GRGCYC 1 cut(s) 180
GlaI GCGC 2 cut(s) 2143, 3521
GsuI CTGGAG 4 cut(s) 2077, 2496, 3101, 3111
HaeII RGCGCY 1 cut(s) 3523
HaeIII GGCC 9 cut(s) 904, 1529, 1665, 1888, 1924, 2253, 2337, 2563, 3144
HapII CCGG 4 cut(s) 1909, 1991, 2092, 3443
HgaI GACGC 2 cut(s) 2690, 3122
HhaI GCGC 2 cut(s) 2144, 3522
Hin1I GRCGYC 1 cut(s) 2701
Hin6I GCGC 2 cut(s) 2142, 3520
HinP1I GCGC 2 cut(s) 2142, 3520
HincII GTYRAC 4 cut(s) 412, 478, 2223, 2967
HindII GTYRAC 4 cut(s) 412, 478, 2223, 2967
HindIII AAGCTT 2 cut(s) 1196, 2577
HpaI GTTAAC 1 cut(s) 2223
HpaII CCGG 4 cut(s) 1909, 1991, 2092, 3443
HpyCH4III ACNGT 8 cut(s) 31, 1058, 1274, 1693, 2801, 2890, 3278, 3363
HpyCH4IV ACGT 4 cut(s) 92, 609, 1698, 2957
HpyF10VI GCNNNNNNNGC 9 cut(s) 281, 1649, 2504, 2849, 2858, 2900, 3247, 3256, 3265
HpySE526I ACGT 4 cut(s) 92, 609, 1698, 2957
Hsp92I GRCGYC 1 cut(s) 2701
HspAI GCGC 2 cut(s) 2142, 3520
KpnI GGTACC 2 cut(s) 1909, 3391
KspAI GTTAAC 1 cut(s) 2223
KspI CCGCGG 1 cut(s) 3526
LguI GCTCTTC 1 cut(s) 2645
LmnI GCTCC 8 cut(s) 226, 1077, 1414, 2947, 3037, 3092, 3247, 3256
Lsp1109I GCAGC 7 cut(s) 296, 1090, 1639, 1999, 2171, 2174, 2864
LweI GCATC 6 cut(s) 271, 1135, 2085, 2494, 2995, 3386
MabI ACCWGGT 1 cut(s) 3390
MaeII ACGT 4 cut(s) 92, 609, 1698, 2957
MbiI CCGCTC 2 cut(s) 2240, 3259
MfeI CAATTG 1 cut(s) 1544
MflI RGATCY 7 cut(s) 490, 949, 1207, 1828, 2629, 3076, 3319
MhlI GDGCHC 5 cut(s) 180, 1124, 2952, 3134, 3283
MlsI TGGCCA 2 cut(s) 1888, 3144
MluI ACGCGT 1 cut(s) 3114
MluNI TGGCCA 2 cut(s) 1888, 3144
MlyI GAGTC 3 cut(s) 167, 269, 2653
MmeI TCCRAC 3 cut(s) 717, 1032, 3252
Mox20I TGGCCA 2 cut(s) 1888, 3144
Mph1103I ATGCAT 1 cut(s) 238
MscI TGGCCA 2 cut(s) 1888, 3144
MslI CAYNNNNRTG 4 cut(s) 2111, 2526, 2571, 3382
Msp20I TGGCCA 2 cut(s) 1888, 3144
MspA1I CMGCKG 2 cut(s) 3491, 3525
MspCI CTTAAG 1 cut(s) 1342
MspI CCGG 4 cut(s) 1909, 1991, 2092, 3443
MspR9I CCNGG 9 cut(s) 679, 1564, 1992, 2475, 3054, 3090, 3146, 3392, 3481
MunI CAATTG 1 cut(s) 1544
Mva1269I GAATGC 2 cut(s) 942, 2535
MvaI CCWGG 8 cut(s) 679, 1564, 2475, 3054, 3090, 3146, 3392, 3481
MvnI CGCG 2 cut(s) 3116, 3525
MwoI GCNNNNNNNGC 9 cut(s) 281, 1649, 2504, 2849, 2858, 2900, 3247, 3256, 3265
NciI CCSGG 1 cut(s) 1992
NcoI CCATGG 1 cut(s) 855
NdeI CATATG 2 cut(s) 1684, 3067
NmuCI GTSAC 4 cut(s) 2364, 2588, 3056, 3394
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 6 cut(s) 71, 510, 566, 2221, 2786, 3209
OliI CACNNNNGTG 1 cut(s) 2111
PaeI GCATGC 1 cut(s) 566
PasI CCCWGGG 1 cut(s) 678
PceI AGGCCT 1 cut(s) 1924
PciI ACATGT 2 cut(s) 67, 2782
PciSI GCTCTTC 1 cut(s) 2645
PctI GAATGC 2 cut(s) 942, 2535
PfeI GAWTC 9 cut(s) 5, 346, 2419, 2458, 2488, 3085, 3332, 3507, 3542
PflMI CCANNNNNTGG 1 cut(s) 1895
PfoI TCCNGGA 2 cut(s) 2473, 3088
PleI GAGTC 3 cut(s) 166, 268, 2653
PpsI GAGTC 3 cut(s) 166, 268, 2653
PpuMI RGGWCCY 3 cut(s) 338, 461, 2602
PscI ACATGT 2 cut(s) 67, 2782
PsiI TTATAA 2 cut(s) 972, 2726
Psp124BI GAGCTC 1 cut(s) 180
Psp5II RGGWCCY 3 cut(s) 338, 461, 2602
Psp6I CCWGG 8 cut(s) 677, 1562, 2473, 3052, 3088, 3144, 3390, 3479
PspEI GGTNACC 1 cut(s) 1348
PspGI CCWGG 8 cut(s) 677, 1562, 2473, 3052, 3088, 3144, 3390, 3479
PspPI GGNCC 9 cut(s) 147, 338, 461, 852, 1663, 2251, 2562, 2602, 3213
PspPPI RGGWCCY 3 cut(s) 338, 461, 2602
PsrI GAACNNNNNNTAC 2 cut(s) 2193, 2225
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 7 cut(s) 490, 949, 1207, 1828, 2629, 3076, 3319
RsaI GTAC 8 cut(s) 95, 1517, 1907, 2325, 2924, 2960, 3389, 3575
RsaNI GTAC 8 cut(s) 94, 1516, 1906, 2324, 2923, 2959, 3388, 3574
RseI CAYNNNNRTG 4 cut(s) 2111, 2526, 2571, 3382
SacI GAGCTC 1 cut(s) 180
SacII CCGCGG 1 cut(s) 3526
SalI GTCGAC 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 2645
Sau96I GGNCC 9 cut(s) 147, 338, 461, 852, 1663, 2251, 2562, 2602, 3213
ScaI AGTACT 1 cut(s) 1517
SchI GAGTC 3 cut(s) 167, 269, 2653
ScrFI CCNGG 9 cut(s) 679, 1564, 1992, 2475, 3054, 3090, 3146, 3392, 3481
SduI GDGCHC 5 cut(s) 180, 1124, 2952, 3134, 3283
SexAI ACCWGGT 1 cut(s) 3390
SfaNI GCATC 6 cut(s) 271, 1135, 2085, 2494, 2995, 3386
SfcI CTRYAG 2 cut(s) 808, 2369
Sfr303I CCGCGG 1 cut(s) 3526
SgrBI CCGCGG 1 cut(s) 3526
SinI GGWCC 6 cut(s) 147, 338, 461, 852, 2602, 3213
SmiMI CAYNNNNRTG 4 cut(s) 2111, 2526, 2571, 3382
SmlI CTYRAG 4 cut(s) 153, 1342, 2327, 2679
SmoI CTYRAG 4 cut(s) 153, 1342, 2327, 2679
SphI GCATGC 1 cut(s) 566
SseBI AGGCCT 1 cut(s) 1924
SsiI CCGC 8 cut(s) 661, 883, 1280, 2238, 3259, 3491, 3523, 3525
SspI AATATT 3 cut(s) 196, 1639, 3328
SstI GAGCTC 1 cut(s) 180
StuI AGGCCT 1 cut(s) 1924
StyD4I CCNGG 9 cut(s) 677, 1562, 1990, 2473, 3052, 3088, 3144, 3390, 3479
StyI CCWWGG 2 cut(s) 752, 855
TaaI ACNGT 8 cut(s) 31, 1058, 1274, 1693, 2801, 2890, 3278, 3363
TaiI ACGT 4 cut(s) 95, 612, 1701, 2960
TaqI TCGA 6 cut(s) 411, 868, 1821, 2595, 3569, 3586
TatI WGTACW 1 cut(s) 1515
TauI GCSGC 3 cut(s) 664, 2240, 3525
TfiI GAWTC 9 cut(s) 5, 346, 2419, 2458, 2488, 3085, 3332, 3507, 3542
TscAI CASTG 5 cut(s) 36, 538, 2137, 2345, 3103
TseFI GTSAC 4 cut(s) 2364, 2588, 3056, 3394
TseI GCWGC 7 cut(s) 284, 1103, 1652, 1987, 2159, 2162, 2852
Tsp45I GTSAC 4 cut(s) 2364, 2588, 3056, 3394
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 5 cut(s) 36, 538, 2137, 2345, 3103
Van91I CCANNNNNTGG 1 cut(s) 1895
Vha464I CTTAAG 1 cut(s) 1342
VneI GTGCAC 1 cut(s) 1120
VpaK11BI GGWCC 6 cut(s) 147, 338, 461, 852, 2602, 3213
XapI RAATTY 7 cut(s) 169, 512, 1139, 1965, 2728, 2973, 3293
XbaI TCTAGA 1 cut(s) 2308
XceI RCATGY 6 cut(s) 71, 510, 566, 2221, 2786, 3209
XmiI GTMKAC 6 cut(s) 64, 411, 1695, 2230, 2357, 3358
ZrmI AGTACT 1 cut(s) 1517
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.