FvH4_2g29170
ERF Family

Probably involved in the RNA silencing pathway and required for the generation of small interfering RNAs (siRNAs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008702
Physical Location & Seq
Reverse (-)
22685620 .. 22691773
6154 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29170.t2

Sequence Viewer

Length: 3603 bp
ATGGGGAAGATGGAATTGGAAGAAAGGGAGAATGACACCGTGGTAACTCAAGTCAGCTTTGGTGGATTTGGAAGTCATGTTACAGCAAAAGAACTTGTAAGCTACTTGGAAGATGAAATTGGGCTTGTATTTAGGTGTCGTCTGAAAACTTCTTGGACCCCTCCAGAGTCTGTTCCAAATTTTGAGATCAGTGATGCTGCAGATATTGAAACTACAGATGATTATAGAAAGGTTGAGCCACATGCATTTGTGCATTTTGCTTCGCCTGAGTCAGTGACTGCTGTTATGGATGCTGTTGGGCGTTGTCAACTCTTTTTCAACAACCAAGCACTGAAAGCAAGTTTGGGCCCTGAGAATTTATTCAACATGAACAAGAGGAGGAGGACTAAAATTCCATTCAAGTTAGCTGATGTGCGTCTTGAGATTGGAAGTTTAGTTCGCCTAGATGAGTTCTTTGTTGCTTGGAAGGGACCAGATCATGGGGTTGATTTTGTTGTGGATCCTTTTGATGGGACATGCAAGTTTTGTTTCAGAAGAGATACTGCCTTTTCATTTAAAGGGTTGAATAAGCATGCTGTGATAACATGTGATTTTAAGGCGGAGTTCCAGGTAAGAGAAATTGGTGAATTCAAACACTACACAGAGCCTTTATATCATGTGCTTCTGTTGAGGCTGGTTTCATCACCTTGGGTTTCATATCGAACTGCTGATGATGATATTGATCAATCAGTTCTATTTGATTTATTGGATGACGATGATCCTTGGATCAGAACCACAGATTTCACTCCTACTGGGGCAATTGGGCGTTGCAATTATTACAGAGTTTTAATCCCACCACGGCATGGTGCTAAGTTGAAGAAGGCCATGATTTATCTTAAAGAAAGAAGGGTGCAAGAGTTGTTTATCAAAAAGCCACCTAGGATTCAGGATGAACCTGGTTTTGGAAATCCCATGTCAGATGCTCTCTTCTCTATTCATTACCAGAAAAATATTTCCTTTGATATATTGTTCTTGGTCAACGCAGTAATGCACAAGGGCATTCTAAATCAGCACCAGTTGTCAGAGAGCTTTTTTGATTTGCTGAGAAACCAACCAGAGGAGATCAATGTGGCTGCATTGAAGCACTTGTGTTCATACAAACACCCAGTTTTTGATGCCTCTAAAAGGCTGAAACTTGTCCAAGAATGGTTGCTGAGAAACCCCAAGCTGATCAAGAATCCCAAACGCTTGGATGATATTGCTGAAGTTAGGAGGTTGGTGATTACCCCAACTAAAGCCTATTGTCTTCCACCAGAGGTTGAACTGTCCAATAGGGTTCTTAGAAAATATAAAGAAGTTGCTGATAGGTTTTTAAGGGTTACCTTTATGGATGAAGGAATGCAAACAATGAATTCCAATGTTATGAATTCTTATGTTGCTCCTATTGTGAAGGAAATTACAGAAAACACTTTCCCGCAGAAAACAAAAGTGTATGTAAGGATTCGGGCCATTTTGATGAATGGATTTCATTTATGCGGTCGTAAGTACTCATTCTTGGCCTTCTCATCCAATCAACTGAGGGACCGTTCTGCCTGGTTTTTCGCTGAAGACAAGACGATCAGTATACAAGGAATCAAAAATTGGATGGGAAGGTTTACAAACAGAAACGTTGCAAAGTGTGCTGCTAGGATGGGTCAGTGCTTTTCGTCTACATATGCAACAGTCGAAGTTCCATCAACCCAGGTCAATCATGAACTTCCAGATATTGAGAGAAATGGATACGTCTTTTCTGATGGTATTGGTAGGATCACCCCAGACCTTGCAATGGAAGTTGCAGAGAAACTGAAATTGGACAGAGATCCACCTTCTGCATATCAAATTAGATACGGAGGCTGTAAAGGGGTTGTTGCTTGTTGGCCATCGACAGGTGATGGCTTCCGGCTTTCTTTGAGGCGTAGTATGAACAAATTTGCATCAGACCATACTACATTGGAAATATGTTCATGGACTAGGTTGCAGCCAGGTTTCCTAAACAGGCAGATTATAACATTGCTTTCAGCACTTAATGTTCCAGATGAAATATTCTGGACAATGCAGGAGACAATGGTTTCCAAATTAAAGCAGATGCTGGTGGACACGGATGTGGCATTTGACGTTCTCACTGCGTCCTGTGCTGAGCAAGGAAATTCTGCAGCTATTATGTTGAGTGCAGGTTTCAAGCCCCAGACAGAACCACATCTGAGAGGCATGTTAACCTGTATACAAGCCGCGCAGCTTTGGGGACTTAGGGAAAAGACCAGGATTTTTGTTCCTTCTGGGAGATGGCTTATGGGCTGTTTAGATGAGTTAGGGGTACTTGAGCAAGGCCAGTGCTTTGTCCAAGTGTCTACGCCATCGCTGGAAAACTGTTTTGCAAAGCATGGCTCTAGGTTTGATCAGACAGATAAAAATCTTCAAGTAATAAAAGGATTCGTAGTGGTCGCAAAGAATCCTTGCCTTCACCCAGGAGATATCAGAATTCTTGAAGCAGTTGATGCACCTGGTTTACACCACTTGCATGATTGCCTTGTTTTCCCTCAGAAAGGTCATAGGCCGCACACAGATGAAGCTTCTGGAAGTGACCTAGATGGGGACCTGTACTTTGTCACATGGGATGAAAATCTTATTCCCCCACGTAAGAAGAGCTGGCAACCTATGGAGTATGAACCTGCAGAAGCGAAACTTTCAGGGCGTAGTGTCACTAGCCAAGATTTGATTGAGTTTTTTGTCAGAAACATGGTGAATGAGAACCTGGGAGCGATATGCAATGCCCATGTGGTTCATTCTGACCGCAGTGAATATGGAGCTTTCGATGAGAATTGCCTCAAACTAGCAGAGTTAGCAGCTGTAGCAGTTGATTTTCCTAAAACTGGGAAGATTGTTACAATGCCACCACACCTAAAGCCAAAGCTGTACCCAGATTTCATGGGAAAACAGGACTACCAGTCGTACAAGTCCAACAAAATTCTAGGGAGACTCTATCGCAAGATTTGCGATGCCTATGATGAAGATGTGGCAAACTCTTCGGAGCAAAATCATGTTCCCAGTGACGTCAAATATGACATGGATCTGGAGGTTCCAAAAGCCGCAGATTTCATAGCTGATGCTTGGGAGAGAAAATGCTCCTATGATAGTCAGCTACGGGGTCTAATGACACAGTATAAAATTATGAGGGAAGAAGAGATTGTCACAGGGCATGTTTGGTCAATGCCCAAGTATACCAGCAGGAAGCAAGGAGAGCTGCAGGAGCGGTTGAAGCATTCTTACAGTGCTCTGAAGAAAGAGTTCAGGCAGTTTTTTGAGAAGATGGACACGGGTTTTGAGTCGCTGATTGATGATCAGAAAAATAGTTTTTATGAGCAGAAGGCATCAGCTTGGTACCAGGTGACTTACCACCCTGATTGGGTGAAAAAATCACTCGATTTGCATGGGCCGGATGGTCCAGGAGATGTGGTGATGTTGAGCTTTGCCTGGATTGCAGCTGACTACCTTGCTCGGATCAAGATCAAGCGCCGTGAAGTGAGTAATATCGACCCTAAGAAGCCGATCAACATTTTGACGAAGTACCTTGCTGATAGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001172 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0003968 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009791 GO:0009856 GO:0009875 GO:0009888 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010073 GO:0010074 GO:0010267 GO:0010467 GO:0010468 GO:0010492 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0019827 GO:0022414 GO:0030422 GO:0031047 GO:0031050 GO:0032501 GO:0032502 GO:0032774 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0044706 GO:0045087 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048507 GO:0048519 GO:0048544 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090567 GO:0097659 GO:0097747 GO:0098542 GO:0098586 GO:0098727 GO:0099402 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RdRP

Protein Analysis

1201

Amino Acids

137.31

Weight (kDa)

7.17

Isoelectric Point (pI)

34.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_RDR6 PF24572 9 - 110 1.8e-31 RNA-dependent RNA polymerase 6, RNA-binding domain
RDR6_2nd PF24577 131 - 294 5.1e-79 RNA-dependent RNA polymerase 6 second domain
RdRP_helical PF26252 319 - 400 2.6e-22 RNA dependent RNA polymerase, helical domain
RdRP PF05183 418 - 1000 4e-194 RNA dependent RNA polymerase
RdRP_head PF26253 1023 - 1177 1.1e-45 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2024
AatII GACGTC 1 cut(s) 3072
Acc36I ACCTGC 2 cut(s) 2180, 2695
Acc65I GGTACC 1 cut(s) 3396
AccB1I GGYRCC 1 cut(s) 3396
AccB7I CCANNNNNTGG 2 cut(s) 479, 842
AccBSI CCGCTC 1 cut(s) 3268
AccI GTMKAC 5 cut(s) 1603, 1688, 2239, 2366, 3236
AccII CGCG 1 cut(s) 2249
AciI CCGC 8 cut(s) 599, 1454, 1515, 2247, 2573, 2809, 3105, 3268
AclI AACGTT 1 cut(s) 1647
AclWI GGATC 8 cut(s) 494, 507, 752, 773, 1793, 1832, 3093, 3524
AcoI YGGCCR 1 cut(s) 1895
AcuI CTGAAG 3 cut(s) 1263, 1605, 3314
AcyI GRCGYC 1 cut(s) 3069
AfaI GTAC 7 cut(s) 1526, 2334, 2618, 2933, 2969, 3398, 3584
AflIII ACRYGT 1 cut(s) 584
AhdI GACNNNNNGTC 1 cut(s) 2962
AleI CACNNNNGTG 1 cut(s) 2120
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
Alw21I GWGCWC 1 cut(s) 3292
Alw26I GTCTC 2 cut(s) 2072, 2986
AlwI GGATC 8 cut(s) 494, 507, 752, 773, 1793, 1832, 3093, 3524
AlwNI CAGNNNCTG 3 cut(s) 170, 278, 2107
AoxI GGCC 8 cut(s) 346, 861, 1485, 1536, 1895, 2344, 2570, 3449
ApaI GGGCCC 1 cut(s) 350
ApeKI GCWGC 9 cut(s) 197, 1112, 1661, 1996, 2171, 2251, 2861, 3259, 3497
ArsI GACNNNNNNTTYG 4 cut(s) 2603, 2635, 3320, 3352
Asp700I GAANNNNTTC 2 cut(s) 359, 3302
Asp718I GGTACC 1 cut(s) 3396
AspA2I CCTAGG 1 cut(s) 917
AspLEI GCGC 2 cut(s) 2251, 3531
AspS9I GGNCC 9 cut(s) 156, 346, 347, 470, 1485, 1561, 2611, 3449, 3458
AvaII GGWCC 5 cut(s) 156, 470, 1561, 2611, 3458
AvrII CCTAGG 1 cut(s) 917
BaeGI GKGCMC 1 cut(s) 350
BalI TGGCCA 1 cut(s) 1897
BamHI GGATCC 1 cut(s) 499
BanI GGYRCC 1 cut(s) 3396
BanII GRGCYC 1 cut(s) 350
BarI GAAGNNNNNNTAC 4 cut(s) 64, 96, 3266, 3298
BbsI GAAGAC 2 cut(s) 1277, 1593
Bbv12I GWGCWC 1 cut(s) 3292
BbvI GCAGC 9 cut(s) 184, 1099, 1648, 2008, 2183, 2263, 2873, 3246, 3509
BceAI ACGGC 2 cut(s) 854, 3516
BcgI CGANNNNNNTGC 2 cut(s) 3024, 3058
BciVI GTATCC 1 cut(s) 1751
BclI TGATCA 4 cut(s) 721, 1209, 2413, 3355
BcoDI GTCTC 2 cut(s) 2072, 2986
BfaI CTAG 9 cut(s) 443, 918, 1665, 1989, 2406, 2603, 2721, 2849, 2987
BfmI CTRYAG 6 cut(s) 198, 213, 2169, 2688, 2865, 3260
BfoI RGCGCY 1 cut(s) 3532
BfuAI ACCTGC 2 cut(s) 2180, 2695
BfuI GTATCC 1 cut(s) 1751
BlnI CCTAGG 1 cut(s) 917
BlpI GCTNAGC 1 cut(s) 2154
BmcAI AGTACT 1 cut(s) 1526
Bme18I GGWCC 5 cut(s) 156, 470, 1561, 2611, 3458
BmeRI GACNNNNNGTC 1 cut(s) 2962
BmgT120I GGNCC 9 cut(s) 156, 346, 347, 470, 1485, 1561, 2611, 3449, 3458
BmiI GGNNCC 8 cut(s) 158, 348, 471, 501, 1562, 2612, 3096, 3398
BmrI ACTGGG 4 cut(s) 801, 1139, 2898, 3057
BmuI ACTGGG 4 cut(s) 801, 1139, 2898, 3057
BpiI GAAGAC 2 cut(s) 1277, 1593
BpmI CTGGAG 2 cut(s) 147, 3110
Bpu1102I GCTNAGC 1 cut(s) 2154
BpuEI CTTGAG 3 cut(s) 33, 440, 2357
BsaAI YACGTR 1 cut(s) 2654
BsaBI GATNNNNATC 4 cut(s) 720, 2427, 2637, 3521
BsaHI GRCGYC 1 cut(s) 3069
BsaJI CCNNGG 8 cut(s) 39, 686, 761, 836, 917, 1719, 2482, 2770
BsaXI ACNNNNNCTCC 2 cut(s) 3456, 3486
Bse1I ACTGG 7 cut(s) 796, 1054, 1145, 2347, 2893, 2962, 3063
Bse3DI GCAATG 3 cut(s) 1809, 2027, 2791
Bse8I GATNNNNATC 4 cut(s) 720, 2427, 2637, 3521
BseDI CCNNGG 8 cut(s) 39, 686, 761, 836, 917, 1719, 2482, 2770
BseJI GATNNNNATC 4 cut(s) 720, 2427, 2637, 3521
BseMI GCAATG 3 cut(s) 1809, 2027, 2791
BseMII CTCAG 8 cut(s) 258, 342, 1073, 1184, 1547, 2145, 2210, 2570
BseNI ACTGG 7 cut(s) 796, 1054, 1145, 2347, 2893, 2962, 3063
BseRI GAGGAG 3 cut(s) 391, 394, 1112
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 9 cut(s) 184, 1099, 1648, 2008, 2183, 2263, 2873, 3246, 3509
BsgI GTGCAG 1 cut(s) 2208
Bsh1236I CGCG 1 cut(s) 2249
Bsh1285I CGRYCG 1 cut(s) 1519
BshFI GGCC 8 cut(s) 348, 863, 1487, 1538, 1897, 2346, 2572, 3451
BshNI GGYRCC 1 cut(s) 3396
BsiEI CGRYCG 1 cut(s) 1519
BsiHKAI GWGCWC 1 cut(s) 3292
BsiSI CCGG 2 cut(s) 1918, 3452
BslFI GGGAC 5 cut(s) 483, 526, 1574, 2274, 2624
BsmAI GTCTC 2 cut(s) 2072, 2986
BsmFI GGGAC 5 cut(s) 483, 526, 1574, 2274, 2624
BsmI GAATGC 3 cut(s) 1038, 1383, 3277
BsnI GGCC 8 cut(s) 348, 863, 1487, 1538, 1897, 2346, 2572, 3451
Bsp120I GGGCCC 1 cut(s) 346
Bsp1286I GDGCHC 2 cut(s) 350, 3292
Bsp1720I GCTNAGC 1 cut(s) 2154
BspACI CCGC 8 cut(s) 599, 1454, 1515, 2247, 2573, 2809, 3105, 3268
BspANI GGCC 8 cut(s) 348, 863, 1487, 1538, 1897, 2346, 2572, 3451
BspCNI CTCAG 8 cut(s) 259, 343, 1074, 1185, 1548, 2146, 2211, 2569
BspFNI CGCG 1 cut(s) 2249
BspHI TCATGA 1 cut(s) 1729
BspLI GGNNCC 8 cut(s) 158, 348, 471, 501, 1562, 2612, 3096, 3398
BspMAI CTGCAG 4 cut(s) 202, 2173, 2692, 3264
BspMI ACCTGC 2 cut(s) 2180, 2695
BspPI GGATC 8 cut(s) 494, 507, 752, 773, 1793, 1832, 3093, 3524
BspQI GCTCTTC 1 cut(s) 2654
BspT107I GGYRCC 1 cut(s) 3396
BsrBI CCGCTC 1 cut(s) 3268
BsrDI GCAATG 3 cut(s) 1809, 2027, 2791
BsrI ACTGG 7 cut(s) 796, 1054, 1145, 2347, 2893, 2962, 3063
BssECI CCNNGG 8 cut(s) 39, 686, 761, 836, 917, 1719, 2482, 2770
BssNAI GTATAC 3 cut(s) 1604, 2240, 3237
BssNI GRCGYC 1 cut(s) 3069
BssT1I CCWWGG 3 cut(s) 686, 761, 917
Bst1107I GTATAC 3 cut(s) 1604, 2240, 3237
Bst4CI ACNGT 7 cut(s) 40, 1305, 1565, 1702, 2387, 3177, 3287
Bst6I CTCTTC 5 cut(s) 529, 971, 2654, 3046, 3192
BstACI GRCGYC 1 cut(s) 3069
BstAPI GCANNNNNTGC 3 cut(s) 1658, 2513, 3009
BstBAI YACGTR 1 cut(s) 2654
BstC8I GCNNGC 2 cut(s) 573, 2666
BstDSI CCRYGG 2 cut(s) 39, 836
BstEII GGTNACC 1 cut(s) 1357
BstENI CCTNNNNNAGG 2 cut(s) 1162, 2559
BstFNI CGCG 1 cut(s) 2249
BstH2I RGCGCY 1 cut(s) 3532
BstHHI GCGC 2 cut(s) 2251, 3531
BstMAI GTCTC 2 cut(s) 2072, 2986
BstMCI CGRYCG 1 cut(s) 1519
BstNSI RCATGY 6 cut(s) 245, 519, 575, 588, 2230, 3218
BstPI GGTNACC 1 cut(s) 1357
BstSFI CTRYAG 6 cut(s) 198, 213, 2169, 2688, 2865, 3260
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 2249
BstV1I GCAGC 9 cut(s) 184, 1099, 1648, 2008, 2183, 2263, 2873, 3246, 3509
BstV2I GAAGAC 2 cut(s) 1277, 1593
BstX2I RGATCY 3 cut(s) 499, 1837, 3085
BstXI CCANNNNNNTGG 1 cut(s) 1228
BstYI RGATCY 3 cut(s) 499, 1837, 3085
BstZ17I GTATAC 3 cut(s) 1604, 2240, 3237
BsuI GTATCC 1 cut(s) 1751
BsuRI GGCC 8 cut(s) 348, 863, 1487, 1538, 1897, 2346, 2572, 3451
BtgI CCRYGG 2 cut(s) 39, 836
BtgZI GCGATG 2 cut(s) 2358, 3027
BtsI GCAGTG 2 cut(s) 2139, 2818
BtsIMutI CAGTG 9 cut(s) 196, 279, 329, 1682, 2139, 2354, 2818, 3070, 3292
BveI ACCTGC 2 cut(s) 2180, 2695
Cac8I GCNNGC 2 cut(s) 573, 2666
CaiI CAGNNNCTG 3 cut(s) 170, 278, 2107
CciI TCATGA 1 cut(s) 1729
CfoI GCGC 2 cut(s) 2251, 3531
Cfr13I GGNCC 9 cut(s) 156, 346, 347, 470, 1485, 1561, 2611, 3449, 3458
CseI GACGC 2 cut(s) 404, 2133
CsiI ACCWGGT 3 cut(s) 934, 2518, 3399
Csp6I GTAC 7 cut(s) 1525, 2333, 2617, 2932, 2968, 3397, 3583
CspCI CAANNNNNGTGG 2 cut(s) 3401, 3436
CviQI GTAC 7 cut(s) 1525, 2333, 2617, 2932, 2968, 3397, 3583
DraI TTTAAA 1 cut(s) 556
DriI GACNNNNNGTC 1 cut(s) 2962
EaeI YGGCCR 1 cut(s) 1895
Eam1104I CTCTTC 5 cut(s) 529, 971, 2654, 3046, 3192
Eam1105I GACNNNNNGTC 1 cut(s) 2962
EarI CTCTTC 5 cut(s) 529, 971, 2654, 3046, 3192
EciI GGCGGA 1 cut(s) 614
Eco130I CCWWGG 3 cut(s) 686, 761, 917
Eco24I GRGCYC 1 cut(s) 350
Eco32I GATATC 1 cut(s) 2491
Eco47I GGWCC 5 cut(s) 156, 470, 1561, 2611, 3458
Eco57I CTGAAG 3 cut(s) 1263, 1605, 3314
Eco91I GGTNACC 1 cut(s) 1357
EcoNI CCTNNNNNAGG 2 cut(s) 1162, 2559
EcoO109I RGGNCCY 2 cut(s) 347, 2611
EcoO65I GGTNACC 1 cut(s) 1357
EcoRI GAATTC 4 cut(s) 626, 1390, 1405, 2496
EcoRV GATATC 1 cut(s) 2491
EcoT14I CCWWGG 3 cut(s) 686, 761, 917
EcoT22I ATGCAT 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 3 cut(s) 686, 761, 917
FalI AAGNNNNNCTT 2 cut(s) 2685, 2717
FaqI GGGAC 5 cut(s) 483, 526, 1574, 2274, 2624
FauI CCCGC 1 cut(s) 1461
FauNDI CATATG 1 cut(s) 1693
FbaI TGATCA 4 cut(s) 721, 1209, 2413, 3355
FblI GTMKAC 5 cut(s) 1603, 1688, 2239, 2366, 3236
FriOI GRGCYC 1 cut(s) 350
FspBI CTAG 9 cut(s) 443, 918, 1665, 1989, 2406, 2603, 2721, 2849, 2987
GlaI GCGC 2 cut(s) 2250, 3530
GsuI CTGGAG 2 cut(s) 147, 3110
HaeII RGCGCY 1 cut(s) 3532
HaeIII GGCC 8 cut(s) 348, 863, 1487, 1538, 1897, 2346, 2572, 3451
HapII CCGG 2 cut(s) 1918, 3452
HgaI GACGC 2 cut(s) 404, 2133
HhaI GCGC 2 cut(s) 2251, 3531
Hin1I GRCGYC 1 cut(s) 3069
Hin6I GCGC 2 cut(s) 2249, 3529
HinP1I GCGC 2 cut(s) 2249, 3529
HincII GTYRAC 3 cut(s) 308, 1018, 2232
HindII GTYRAC 3 cut(s) 308, 1018, 2232
HindIII AAGCTT 1 cut(s) 2586
HpaI GTTAAC 1 cut(s) 2232
HpaII CCGG 2 cut(s) 1918, 3452
HpyCH4III ACNGT 7 cut(s) 40, 1305, 1565, 1702, 2387, 3177, 3287
HpyCH4IV ACGT 5 cut(s) 1647, 1761, 2133, 2653, 3069
HpySE526I ACGT 5 cut(s) 1647, 1761, 2133, 2653, 3069
Hsp92I GRCGYC 1 cut(s) 3069
HspAI GCGC 2 cut(s) 2249, 3529
KpnI GGTACC 1 cut(s) 3400
Ksp22I TGATCA 4 cut(s) 721, 1209, 2413, 3355
KspAI GTTAAC 1 cut(s) 2232
LguI GCTCTTC 1 cut(s) 2654
LmnI GCTCC 6 cut(s) 1423, 2774, 2822, 3046, 3146, 3265
Lsp1109I GCAGC 9 cut(s) 184, 1099, 1648, 2008, 2183, 2263, 2873, 3246, 3509
MabI ACCWGGT 3 cut(s) 934, 2518, 3399
MaeI CTAG 9 cut(s) 443, 918, 1665, 1989, 2406, 2603, 2721, 2849, 2987
MaeII ACGT 5 cut(s) 1647, 1761, 2133, 2653, 3069
MbiI CCGCTC 1 cut(s) 3268
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 3 cut(s) 499, 1837, 3085
MhlI GDGCHC 2 cut(s) 350, 3292
MlsI TGGCCA 1 cut(s) 1897
MluNI TGGCCA 1 cut(s) 1897
MlyI GAGTC 4 cut(s) 176, 278, 2988, 3350
MmeI TCCRAC 1 cut(s) 3000
Mox20I TGGCCA 1 cut(s) 1897
Mph1103I ATGCAT 1 cut(s) 247
MroXI GAANNNNTTC 2 cut(s) 359, 3302
MscI TGGCCA 1 cut(s) 1897
MseI TTAA 8 cut(s) 555, 594, 827, 876, 1352, 2043, 2096, 2231
MslI CAYNNNNRTG 4 cut(s) 1493, 2120, 2535, 2580
Msp20I TGGCCA 1 cut(s) 1897
MspA1I CMGCKG 2 cut(s) 2864, 3500
MspI CCGG 2 cut(s) 1918, 3452
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 3 cut(s) 1038, 1383, 3277
MvnI CGCG 1 cut(s) 2249
NdeI CATATG 1 cut(s) 1693
NlaIV GGNNCC 8 cut(s) 158, 348, 471, 501, 1562, 2612, 3096, 3398
NmuCI GTSAC 7 cut(s) 274, 2597, 2623, 2716, 3065, 3205, 3403
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 6 cut(s) 245, 519, 575, 588, 2230, 3218
OliI CACNNNNGTG 1 cut(s) 2120
PaeI GCATGC 1 cut(s) 575
PagI TCATGA 1 cut(s) 1729
PciI ACATGT 1 cut(s) 584
PciSI GCTCTTC 1 cut(s) 2654
PctI GAATGC 3 cut(s) 1038, 1383, 3277
PdmI GAANNNNTTC 2 cut(s) 359, 3302
PfeI GAWTC 6 cut(s) 922, 1216, 1480, 1611, 2448, 2467
PflMI CCANNNNNTGG 2 cut(s) 479, 842
PfoI TCCNGGA 1 cut(s) 3460
PleI GAGTC 4 cut(s) 175, 277, 2988, 3349
PpsI GAGTC 4 cut(s) 175, 277, 2988, 3349
Ppu21I YACGTR 1 cut(s) 2654
PpuMI RGGWCCY 1 cut(s) 2611
PscI ACATGT 1 cut(s) 584
PsiI TTATAA 1 cut(s) 2024
Psp1406I AACGTT 1 cut(s) 1647
Psp5II RGGWCCY 1 cut(s) 2611
PspEI GGTNACC 1 cut(s) 1357
PspN4I GGNNCC 8 cut(s) 158, 348, 471, 501, 1562, 2612, 3096, 3398
PspOMI GGGCCC 1 cut(s) 346
PspPI GGNCC 9 cut(s) 156, 346, 347, 470, 1485, 1561, 2611, 3449, 3458
PspPPI RGGWCCY 1 cut(s) 2611
PstI CTGCAG 4 cut(s) 202, 2173, 2692, 3264
PstNI CAGNNNCTG 3 cut(s) 170, 278, 2107
PsuI RGATCY 3 cut(s) 499, 1837, 3085
PvuII CAGCTG 2 cut(s) 2864, 3500
RsaI GTAC 7 cut(s) 1526, 2334, 2618, 2933, 2969, 3398, 3584
RsaNI GTAC 7 cut(s) 1525, 2333, 2617, 2932, 2968, 3397, 3583
RseI CAYNNNNRTG 4 cut(s) 1493, 2120, 2535, 2580
SapI GCTCTTC 1 cut(s) 2654
SaqAI TTAA 8 cut(s) 555, 594, 827, 876, 1352, 2043, 2096, 2231
Sau96I GGNCC 9 cut(s) 156, 346, 347, 470, 1485, 1561, 2611, 3449, 3458
ScaI AGTACT 1 cut(s) 1526
SchI GAGTC 4 cut(s) 176, 278, 2988, 3350
SduI GDGCHC 2 cut(s) 350, 3292
SexAI ACCWGGT 3 cut(s) 934, 2518, 3399
SfcI CTRYAG 6 cut(s) 198, 213, 2169, 2688, 2865, 3260
SinI GGWCC 5 cut(s) 156, 470, 1561, 2611, 3458
SmiMI CAYNNNNRTG 4 cut(s) 1493, 2120, 2535, 2580
SmlI CTYRAG 3 cut(s) 48, 419, 2336
SmoI CTYRAG 3 cut(s) 48, 419, 2336
SphI GCATGC 1 cut(s) 575
SsiI CCGC 8 cut(s) 599, 1454, 1515, 2247, 2573, 2809, 3105, 3268
SspI AATATT 2 cut(s) 991, 2061
SspMI CTAG 9 cut(s) 443, 918, 1665, 1989, 2406, 2603, 2721, 2849, 2987
StyI CCWWGG 3 cut(s) 686, 761, 917
TaaI ACNGT 7 cut(s) 40, 1305, 1565, 1702, 2387, 3177, 3287
TaiI ACGT 5 cut(s) 1650, 1764, 2136, 2656, 3072
TaqI TCGA 6 cut(s) 700, 1704, 1901, 2829, 3438, 3549
TatI WGTACW 2 cut(s) 1524, 2616
TauI GCSGC 3 cut(s) 2249, 2575, 3107
TfiI GAWTC 6 cut(s) 922, 1216, 1480, 1611, 2448, 2467
Tru1I TTAA 8 cut(s) 555, 594, 827, 876, 1352, 2043, 2096, 2231
Tru9I TTAA 8 cut(s) 555, 594, 827, 876, 1352, 2043, 2096, 2231
TscAI CASTG 9 cut(s) 196, 279, 336, 1682, 2146, 2354, 2818, 3070, 3292
TseFI GTSAC 7 cut(s) 274, 2597, 2623, 2716, 3065, 3205, 3403
TseI GCWGC 9 cut(s) 197, 1112, 1661, 1996, 2171, 2251, 2861, 3259, 3497
Tsp45I GTSAC 7 cut(s) 274, 2597, 2623, 2716, 3065, 3205, 3403
TspGWI ACGGA 2 cut(s) 1881, 2132
TspRI CASTG 9 cut(s) 196, 279, 336, 1682, 2146, 2354, 2818, 3070, 3292
Van91I CCANNNNNTGG 2 cut(s) 479, 842
VpaK11BI GGWCC 5 cut(s) 156, 470, 1561, 2611, 3458
XagI CCTNNNNNAGG 2 cut(s) 1162, 2559
XceI RCATGY 6 cut(s) 245, 519, 575, 588, 2230, 3218
XmaJI CCTAGG 1 cut(s) 917
XmiI GTMKAC 5 cut(s) 1603, 1688, 2239, 2366, 3236
XmnI GAANNNNTTC 2 cut(s) 359, 3302
XspI CTAG 9 cut(s) 443, 918, 1665, 1989, 2406, 2603, 2721, 2849, 2987
ZraI GACGTC 1 cut(s) 3070
ZrmI AGTACT 1 cut(s) 1526
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.